##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-LC3_S57_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	303992
Sequences flagged as poor quality	0
Sequence length	61
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.44226163846417	40.0	40.0	40.0	40.0	40.0
2	39.32441643201137	40.0	40.0	40.0	40.0	40.0
3	39.3188899707887	40.0	40.0	40.0	40.0	40.0
4	39.1271743993263	40.0	40.0	40.0	40.0	40.0
5	39.22134793020869	40.0	40.0	40.0	40.0	40.0
6	39.27071765046448	40.0	40.0	40.0	40.0	40.0
7	39.3168109687097	40.0	40.0	40.0	40.0	40.0
8	39.29492881391616	40.0	40.0	40.0	40.0	40.0
9	39.32300850022369	40.0	40.0	40.0	40.0	40.0
10	39.349324982236375	40.0	40.0	40.0	40.0	40.0
11	39.322495328824445	40.0	40.0	40.0	40.0	40.0
12	39.34196952551383	40.0	40.0	40.0	40.0	40.0
13	39.21893997210453	40.0	40.0	40.0	40.0	40.0
14	39.3535224611174	40.0	40.0	40.0	40.0	40.0
15	39.3367193873523	40.0	40.0	40.0	40.0	40.0
16	39.329534987762834	40.0	40.0	40.0	40.0	40.0
17	39.36219374194058	40.0	40.0	40.0	40.0	40.0
18	39.29995526198058	40.0	40.0	40.0	40.0	40.0
19	39.3495881470565	40.0	40.0	40.0	40.0	40.0
20	38.22046632806126	40.0	40.0	40.0	40.0	40.0
21	38.64500381588989	40.0	40.0	40.0	40.0	40.0
22	38.71516355693571	40.0	40.0	40.0	40.0	40.0
23	39.17649148661807	40.0	40.0	40.0	40.0	40.0
24	39.25987525987526	40.0	40.0	40.0	40.0	40.0
25	38.45859101555304	40.0	40.0	40.0	40.0	40.0
26	39.17497828890234	40.0	40.0	40.0	40.0	40.0
27	39.18101792152425	40.0	40.0	40.0	40.0	40.0
28	38.043698518382065	40.0	40.0	40.0	40.0	40.0
29	38.985236453590886	40.0	40.0	40.0	40.0	40.0
30	37.26525698044686	40.0	40.0	40.0	24.0	40.0
31	38.01331613989842	40.0	40.0	40.0	24.0	40.0
32	38.25254611963473	40.0	40.0	40.0	40.0	40.0
33	38.6528921813732	40.0	40.0	40.0	40.0	40.0
34	37.800007894944606	40.0	40.0	40.0	24.0	40.0
35	36.854009316034634	40.0	40.0	40.0	24.0	40.0
36	36.59194978815232	40.0	40.0	40.0	12.0	40.0
37	36.79717887312824	40.0	40.0	40.0	24.0	40.0
38	36.460985815416194	40.0	40.0	40.0	24.0	40.0
39	36.99119713676676	40.0	40.0	40.0	24.0	40.0
40	36.546922287428615	40.0	40.0	40.0	24.0	40.0
41	37.24763809573936	40.0	40.0	40.0	24.0	40.0
42	36.72030842916919	40.0	40.0	40.0	24.0	40.0
43	37.80644227479671	40.0	40.0	40.0	24.0	40.0
44	36.23126924392747	40.0	40.0	40.0	24.0	40.0
45	37.6309113397721	40.0	40.0	40.0	24.0	40.0
46	37.00623700623701	40.0	40.0	40.0	24.0	40.0
47	35.20651859259454	40.0	40.0	40.0	12.0	40.0
48	36.35350930287639	40.0	40.0	40.0	24.0	40.0
49	35.368930761335825	40.0	40.0	40.0	12.0	40.0
50	36.68014947761783	40.0	40.0	40.0	24.0	40.0
51	35.06396220953183	40.0	40.0	40.0	12.0	40.0
52	36.1649385510145	40.0	40.0	40.0	24.0	40.0
53	35.135740414221424	40.0	40.0	40.0	24.0	40.0
54	33.75188820758441	40.0	24.0	40.0	12.0	40.0
55	34.383957472565065	40.0	24.0	40.0	12.0	40.0
56	33.87383878523119	40.0	24.0	40.0	12.0	40.0
57	33.584989078659966	40.0	24.0	40.0	12.0	40.0
58	34.239453669833416	40.0	24.0	40.0	12.0	40.0
59	33.73208505486986	40.0	24.0	40.0	12.0	40.0
60	32.91305034343009	40.0	24.0	40.0	12.0	40.0
61	33.226492802442166	40.0	24.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.17762573019609817
1101	2	-0.18515628240081838
1101	3	-0.07852176900958341
1101	4	0.059908419277981295
1101	5	-0.1410624778725733
1101	6	0.06346250687838051
1101	7	-0.04058923423722405
1101	8	-0.07296251255726816
1101	9	0.05267302243478156
1101	10	0.017054678243496824
1101	11	-0.15599477043514298
1101	12	-0.0066579528692258805
1101	13	-0.004758459479347721
1101	14	0.20897106564409285
1101	15	-0.00658600979856061
1101	16	0.12699783775370577
1101	17	0.15259424037961367
1101	18	0.14197476079812787
1101	19	0.09002352365656208
1101	20	0.0044802872457267995
1101	21	0.16786809501270739
1101	22	0.11761879511142581
1101	23	0.1076170842452342
1101	24	0.2095579219747421
1101	25	-0.12875374490320013
1101	26	0.23420313034275608
1101	27	0.12413314009557297
1101	28	-0.42900249551354364
1101	29	0.2570572420695356
1101	30	-0.11515460382467069
1101	31	0.06594265646502606
1101	32	0.21461598482716937
1101	33	0.1705151049822149
1101	34	0.32313447020065666
1101	35	0.050022182410423
1101	36	-0.10461136677642457
1101	37	0.22059429470228764
1101	38	0.1684511685187502
1101	39	0.12055654751458889
1101	40	0.06763998546826144
1101	41	-0.07519743810923529
1101	42	0.4536601895892076
1101	43	0.04205585035513337
1101	44	-0.10251195240910249
1101	45	0.2406265498968665
1101	46	0.10289072052782444
1101	47	0.13903231700749785
1101	48	0.5203862414368601
1101	49	0.4757056274929994
1101	50	0.3972301789503021
1101	51	0.1589962089563315
1101	52	0.4620647451818485
1101	53	0.09554380516097183
1101	54	-0.11033129002595388
1101	55	0.17138124350370276
1101	56	0.16673189871408312
1101	57	-0.2306235568370738
1101	58	0.22984575646091088
1101	59	0.4178969992452579
1101	60	0.04797891751568528
1101	61	-0.07029419658508118
1102	1	-0.19789263348143038
1102	2	-0.02696898715151974
1102	3	0.14504429292921373
1102	4	-0.11678399895837543
1102	5	0.07930151622482384
1102	6	-0.03974952723843472
1102	7	0.017420918503987082
1102	8	-0.002028351058598332
1102	9	0.23003414560186997
1102	10	0.13639278895917073
1102	11	0.0351648217834466
1102	12	-0.11815164035898107
1102	13	-0.16081104283438208
1102	14	-0.0936436349336347
1102	15	0.059577199213521226
1102	16	0.06447392673004515
1102	17	-0.1167652496042848
1102	18	-0.10635910299824758
1102	19	-0.030471960977692447
1102	20	-0.17442400114613577
1102	21	-0.2002925484871909
1102	22	-0.2132872679204425
1102	23	-0.02815573611425748
1102	24	0.047654170475787794
1102	25	-0.0809799214863105
1102	26	0.0686858002757873
1102	27	-0.09835270618587799
1102	28	0.06052809291464456
1102	29	-0.01893785838087325
1102	30	0.010201851267616746
1102	31	0.012111909540905685
1102	32	-0.24222522025627313
1102	33	-0.0987286476098177
1102	34	-0.3583787318993288
1102	35	0.3053260091386889
1102	36	-0.02844330322373878
1102	37	0.23962666580780478
1102	38	0.2725891017704214
1102	39	0.015184082083095518
1102	40	0.2214164001167589
1102	41	-0.01178620708132172
1102	42	-0.15988944406979755
1102	43	-0.014616892524607294
1102	44	0.2854655045189034
1102	45	0.2129524535326297
1102	46	-0.3122850234867798
1102	47	0.015463361633912598
1102	48	0.2717437445955255
1102	49	-0.08023250444119867
1102	50	0.08525360934230264
1102	51	0.049148564373005854
1102	52	-0.011841295345533354
1102	53	0.0017450789152633206
1102	54	0.08283441698483784
1102	55	0.09826093126994095
1102	56	0.17990024196546983
1102	57	0.3601643897565552
1102	58	0.202724627635547
1102	59	0.19840746544189614
1102	60	0.2902558578048371
1102	61	0.12885127567169974
1103	1	-0.19635367977863893
1103	2	0.03219145408606039
1103	3	-0.10511220633372886
1103	4	0.3342442197983715
1103	5	0.07250801341527335
1103	6	0.060699086268435565
1103	7	0.04346409635835613
1103	8	-0.07469927535546361
1103	9	-0.21802669833568444
1103	10	0.15799340709140353
1103	11	0.12719595371152792
1103	12	0.04821690716843818
1103	13	0.08420143990818474
1103	14	0.11390255227668433
1103	15	0.04849418180145193
1103	16	-0.008375550206380922
1103	17	0.09902836905191492
1103	18	0.11363900519402392
1103	19	-0.008514237566359384
1103	20	-0.11684927739788975
1103	21	-0.04050494110318681
1103	22	0.04532497420118631
1103	23	-0.09638410068898651
1103	24	0.08613226429881848
1103	25	-0.032230799635819096
1103	26	-0.08215291886409659
1103	27	0.060159525201335384
1103	28	-0.26510940909141567
1103	29	0.16445270745031593
1103	30	-0.1735543223493181
1103	31	-0.2391201568126533
1103	32	-0.044469669447948945
1103	33	-0.09161630080107841
1103	34	-0.023430985116732472
1103	35	-0.48771979067617366
1103	36	-0.09526450294868027
1103	37	-0.20310310646318896
1103	38	-0.3507254091388248
1103	39	-0.05672586786901945
1103	40	-0.07652843774575047
1103	41	0.05586398704153339
1103	42	0.19752617652284954
1103	43	0.1588895093314946
1103	44	-0.25912004610874106
1103	45	-0.34271186325376135
1103	46	-0.012873503227964989
1103	47	-0.567783086709639
1103	48	0.5424465511666909
1103	49	0.2921092665671665
1103	50	-0.15931674305471688
1103	51	-0.17116838781846155
1103	52	-0.20558366301730047
1103	53	-0.1487280324388749
1103	54	-0.4355251518955612
1103	55	-0.03027146168615502
1103	56	0.17189513195897632
1103	57	0.3810805561038464
1103	58	0.33382052634518544
1103	59	0.32632767791954365
1103	60	0.28796018101062515
1103	61	0.38967416952109346
1104	1	0.07942811938112015
1104	2	0.022409360678842916
1104	3	-0.03821123150278538
1104	4	-0.2431128930891262
1104	5	-0.11629859516476415
1104	6	-0.1015772992829227
1104	7	-0.024330518913082244
1104	8	-0.14725416068068853
1104	9	-0.048479131053824176
1104	10	-0.03682456815911195
1104	11	-0.08795343136576861
1104	12	-0.13352340309916144
1104	13	-0.2315892956559793
1104	14	-0.1680649824199918
1104	15	-0.18895262207171726
1104	16	-0.09971432166873484
1104	17	-0.09816974122549027
1104	18	-0.10574318411607919
1104	19	-0.16153163630981027
1104	20	0.11461231513476378
1104	21	-0.08372662387173335
1104	22	0.018088557263432392
1104	23	-0.09270927682878494
1104	24	-0.19398260119194788
1104	25	-0.047143696938007906
1104	26	-0.3402319188586489
1104	27	-0.1523045793327995
1104	28	-0.021664316212749668
1104	29	-0.3246170891375044
1104	30	-0.07749451382812822
1104	31	-0.3060298500869223
1104	32	-0.09857196948227909
1104	33	-0.334573160853715
1104	34	-0.3909831186832875
1104	35	0.14931506675003448
1104	36	0.23674253719430283
1104	37	0.39485132877240403
1104	38	-0.020680018743284734
1104	39	0.26692454434987667
1104	40	0.017874866544268286
1104	41	-0.08825188107021376
1104	42	0.017246205511675328
1104	43	-0.41429582842645374
1104	44	0.1346833203001907
1104	45	-0.18641197059960035
1104	46	-0.15222474206426995
1104	47	-0.0903698488432596
1104	48	-0.8353769984385266
1104	49	0.10333753176041682
1104	50	0.04052741477090649
1104	51	-0.22266823252119394
1104	52	-0.7679406941693898
1104	53	-0.4328446634144143
1104	54	0.18434309062973853
1104	55	-0.01850156320153218
1104	56	0.329398169791574
1104	57	0.20808484448815534
1104	58	-0.09307369169218305
1104	59	0.03488755455357051
1104	60	-0.180908982400247
1104	61	-0.331580850395099
1201	1	0.1205493059757714
1201	2	0.06691326699336031
1201	3	-0.0010453604139186723
1201	4	0.19560559349616824
1201	5	0.02351167742386906
1201	6	-0.004517908987317298
1201	7	0.13276774182721596
1201	8	-0.04546865285980317
1201	9	-0.04023815631961014
1201	10	0.061578219941907264
1201	11	0.1959880097036617
1201	12	0.06688952655414226
1201	13	0.1642373619812929
1201	14	-0.025079005511024377
1201	15	0.0544226098571059
1201	16	-0.049004357951481836
1201	17	-0.1273192713115492
1201	18	-0.19634105119677514
1201	19	0.030627104860379006
1201	20	-0.07413287423477755
1201	21	-0.1717962603284633
1201	22	0.18166595955050724
1201	23	0.14770167076218144
1201	24	-0.02877550320648936
1201	25	-0.06572663248244481
1201	26	-0.1416601048862134
1201	27	-0.0022668650911299437
1201	28	0.13625760154267397
1201	29	0.03487175900899331
1201	30	0.12020078813355894
1201	31	0.0451871912445867
1201	32	0.08235068053506467
1201	33	-0.07336377248511639
1201	34	0.17342454749336866
1201	35	0.2680626235829209
1201	36	0.2874084550818594
1201	37	0.1725740492630763
1201	38	0.90625565782819
1201	39	-0.03965645317805411
1201	40	0.4890727479398578
1201	41	0.4057441217707378
1201	42	0.11461391661507747
1201	43	-0.12481342198254453
1201	44	-0.10249466339184465
1201	45	0.01961239775567236
1201	46	-0.02811680775862868
1201	47	1.219760141411598
1201	48	0.8092986866117684
1201	49	0.2837888914001212
1201	50	0.2620603197585467
1201	51	0.29858773431229935
1201	52	-0.08208546180010501
1201	53	0.20615461533512303
1201	54	0.2881503020332019
1201	55	-0.11066746181904108
1201	56	0.6534609571217871
1201	57	0.6460765748836295
1201	58	0.4429156051712013
1201	59	0.255803817925603
1201	60	0.47851434757458833
1201	61	0.4940582270649969
1202	1	-0.19890197963863443
1202	2	0.039207887822193754
1202	3	0.0629249248057846
1202	4	0.11760429521427085
1202	5	-0.13012487106000492
1202	6	-0.195667627856011
1202	7	-0.22471863654258328
1202	8	0.07255974189182979
1202	9	-0.14363097792980994
1202	10	-0.041083910349492214
1202	11	0.0015458305472861866
1202	12	-0.1111120979292366
1202	13	-0.08499361802581973
1202	14	0.06814937793229348
1202	15	0.06435218295722223
1202	16	-0.20258871419829916
1202	17	-0.0845955759271888
1202	18	-0.01201111797021781
1202	19	0.1451542703760964
1202	20	0.17308870464563597
1202	21	0.08923451460665177
1202	22	-0.002916236155158458
1202	23	0.21165455856952775
1202	24	-0.006863462348668747
1202	25	0.15307192396726776
1202	26	-0.1582876817419958
1202	27	0.14878181830532355
1202	28	-0.09708519163362439
1202	29	0.005687599787876252
1202	30	-0.0038384125858215157
1202	31	-0.16377312851984982
1202	32	-0.008289837820839807
1202	33	-0.18800402118112913
1202	34	-0.2908819427200413
1202	35	0.06737912274512325
1202	36	0.12338565142362512
1202	37	0.08847060859857692
1202	38	-0.15433844205687564
1202	39	-0.17400362071622055
1202	40	0.008069091221251767
1202	41	-0.23135781727960136
1202	42	0.02161169953234321
1202	43	0.03256357015708744
1202	44	-0.018865302213953328
1202	45	0.00950450117894519
1202	46	0.15545633803060355
1202	47	-0.23741667175215753
1202	48	0.20370346133158534
1202	49	-0.2696401929414449
1202	50	-0.0810296998896618
1202	51	-0.1770096765178053
1202	52	-0.07794052830715259
1202	53	-0.198196042558898
1202	54	0.003380757943268975
1202	55	-0.2352503316744361
1202	56	0.011287672370727364
1202	57	-0.06810894894702102
1202	58	-0.13558749972322914
1202	59	0.24306456273057364
1202	60	0.5508353907234351
1202	61	-0.004434178420844148
1203	1	-0.08728091878582234
1203	2	-0.06843513591514494
1203	3	-0.14782521627077472
1203	4	-0.2021320471801502
1203	5	-0.14707551464804425
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2403	17	0.13060650045397182
2403	18	0.005948230801145371
2403	19	0.040755443396626845
2403	20	0.027566744218226802
2403	21	0.11207470741346981
2403	22	0.2289777875074961
2403	23	0.05557541339138794
2403	24	0.19973646349485819
2403	25	0.06843916703195418
2403	26	0.06659518474264559
2403	27	0.18836789037192858
2403	28	0.20868215929501588
2403	29	-0.0021729860482366803
2403	30	0.46109568927820277
2403	31	0.24109423252103568
2403	32	-0.00824938883711468
2403	33	0.188724330185714
2403	34	0.2931609332026994
2403	35	-0.22378614506085626
2403	36	0.24814380020846727
2403	37	0.4255050239422289
2403	38	-0.019724224239098476
2403	39	0.04913993946849615
2403	40	0.10253095120035027
2403	41	-0.22998255183314598
2403	42	0.4578674719393945
2403	43	0.14532185377187545
2403	44	0.0784279866254991
2403	45	-0.005084098949147631
2403	46	0.024051072921217553
2403	47	-0.10982709410696145
2403	48	-0.09129097693315202
2403	49	0.11105215882988517
2403	50	0.5205410689781047
2403	51	0.40569838294219807
2403	52	0.8337660817309356
2403	53	0.3965118820711666
2403	54	-0.15277928520231399
2403	55	-0.028947031711517468
2403	56	-0.17539787048833944
2403	57	-0.2721336228270914
2403	58	-0.21043160259848293
2403	59	-0.3931035702117498
2403	60	0.13484455915974536
2403	61	0.08309742234769146
2404	1	-0.0726688429394855
2404	2	-0.033009271097618864
2404	3	0.07638551951569639
2404	4	-0.06271158808718269
2404	5	-0.04109532251739978
2404	6	-0.28153233419003243
2404	7	-0.14798790519027705
2404	8	-0.10165347099054856
2404	9	-0.3130902506170159
2404	10	0.027113765427785097
2404	11	-0.03771423735661017
2404	12	-0.14281393507340567
2404	13	-0.13589816865494697
2404	14	-0.12230201269536423
2404	15	-0.12207972452789306
2404	16	0.035775983789626764
2404	17	-0.043319089569727964
2404	18	-0.11942880035760339
2404	19	-0.0941718986625304
2404	20	0.210479245506491
2404	21	-0.09731757675980646
2404	22	0.07778056328131555
2404	23	-0.02203632181078774
2404	24	0.03867287491400617
2404	25	-0.057583712489844174
2404	26	-0.05808103223491656
2404	27	-0.11191037408236326
2404	28	0.19568274330683977
2404	29	-0.009442215496179074
2404	30	0.08731438452709739
2404	31	0.19016840202187524
2404	32	-0.1530980610690733
2404	33	0.07636045646967915
2404	34	0.12030717663257207
2404	35	-0.09221171790773752
2404	36	-0.018591573730866173
2404	37	-0.2072752158461526
2404	38	-0.04841638130392312
2404	39	0.02729102676108397
2404	40	0.14853734097670923
2404	41	0.067739802078016
2404	42	-0.2554789157757469
2404	43	-0.16703514921689333
2404	44	0.11921262367933139
2404	45	-0.07964523124499578
2404	46	0.01473436674787365
2404	47	-0.45846153706274606
2404	48	-0.23814590152692006
2404	49	-0.13897360639736434
2404	50	-0.07857260720451364
2404	51	-0.09045057442494198
2404	52	-0.40176931969130436
2404	53	0.11637858996219563
2404	54	-0.4220568282302466
2404	55	-0.25096771260059114
2404	56	-0.9142483978166567
2404	57	-0.5436097985802917
2404	58	-0.6763081356295046
2404	59	-0.6100673958326936
2404	60	-0.5388852151368653
2404	61	-0.6537743355278849
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	9.0
15	32.0
16	84.0
17	189.0
18	241.0
19	324.0
20	389.0
21	394.0
22	527.0
23	682.0
24	970.0
25	1238.0
26	1706.0
27	2533.0
28	4397.0
29	6424.0
30	10600.0
31	10472.0
32	7079.0
33	4771.0
34	5240.0
35	7656.0
36	10550.0
37	15041.0
38	30415.0
39	99714.0
40	82309.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.9196941984552	33.79837362001132	24.155887732410488	18.12604444912299
2	36.11180557383089	22.592370851864523	14.78591541882681	26.509908155477774
3	16.320824232216637	31.89491828732335	28.71588726019106	23.068370220268957
4	20.594620911076607	24.722690070791337	30.91693202452696	23.765756993605095
5	22.700597384141687	23.03086923340088	20.029474459854207	34.23905892260323
6	29.2616253059291	24.162149003921154	23.574304586962818	23.001921103186927
7	29.245506460696337	23.544040632648226	25.24704597489408	21.963406931761362
8	21.782152161898996	31.469907102818496	24.957235716729386	21.79070501855312
9	21.39858943656412	32.243611673991424	25.183228506013318	21.174570383431142
10	30.602450064475377	24.142082686386484	24.378273112450326	20.87719413668781
11	23.024619068922867	31.616621490039208	23.859509460775282	21.49924998026264
12	30.54619858417327	24.305902786915446	23.89931313981947	21.248585489091816
13	22.469341298455223	24.181228453380353	23.960827916524117	29.388602331640307
14	31.588989183925893	23.398313113502986	23.247980210005526	21.764717492565595
15	22.412431906102793	23.976617805731728	32.42782704808021	21.183123240085266
16	22.409471301876366	24.184518013631937	24.013460880549488	29.39254980394221
17	31.70182110055528	23.90786599647359	22.828890233953526	21.561422669017606
18	22.66605700150004	24.639135240401064	31.503131661359507	21.191676096739386
19	30.540606331745572	24.46873601936893	24.052935603568514	20.93772204531698
20	23.236466749124975	24.233532461380562	30.990618174162478	21.53938261533198
21	23.19962367430722	32.90941866891234	22.96738072054528	20.923576936235165
22	31.787020711071346	24.112476644122214	23.15817521513724	20.942327429669202
23	31.93143240611595	24.153925103292192	22.726913866154373	21.187728624437487
24	31.300823705886998	25.01677675728309	23.49765783310087	20.184741703729046
25	23.6180557383089	32.42124792757704	23.273967735993054	20.686728598121004
26	24.285507513355615	32.86303586936498	22.50848706544909	20.34296955183031
27	23.14008263375352	33.17620200531593	22.82790336587805	20.855811995052502
28	23.81740308955499	32.399865785941735	23.114095107765994	20.66863601673728
29	24.395378825758574	32.05511987157556	22.90027369141293	20.649227611252925
30	24.17728098107845	32.21795310402905	23.174951972420327	20.42981394247217
31	24.164451696097267	24.574988815495143	31.287336508855496	19.973222979552094
32	32.67191241874786	24.441103713255615	22.461117397826257	20.425866470170266
33	23.644121043057194	25.15008668019777	31.290400044738163	19.915392232006866
34	24.45755151451354	25.047040711597674	22.760138424695388	27.7352693491934
35	24.47794678807337	26.26384904865918	22.49236822021632	26.76583594305113
36	29.795849890786602	28.823455880417903	22.340719492618227	19.039974736177268
37	25.443103765888576	25.03355351456617	29.772165056975187	19.75117766257007
38	32.395589357614675	25.528303376404644	22.545001184241688	19.531106081738994
39	24.804600121055817	26.185886470696595	22.2607831785047	26.74873022974289
40	25.354943551146082	25.525342772178217	29.22774283533777	19.89197084133793
41	25.405931735045662	25.297047290718176	29.626108581804782	19.67091239243138
42	25.224019053132977	25.829298139424722	30.240927392826126	18.705755414616174
43	25.496394641964258	25.91087923366404	30.11460827916524	18.47811784520645
44	25.91647148609174	25.63126661227927	29.41689254980394	19.03536935182505
45	25.829298139424722	26.225361193715624	30.156714650385535	17.78862601647412
46	26.360562120055793	24.75196715703045	21.436419379457355	27.451051343456406
47	28.533645622253218	24.78519171557146	20.775217768888655	25.905944893286666
48	26.158254164583276	33.865693834048265	21.16174109844996	18.8143109029185
49	27.109266033316665	24.85690412905603	21.29858680491592	26.735243032711388
50	26.814192478749444	25.675346719650516	21.2844416958341	26.226019105765943
51	26.728663912208216	25.841469512355587	22.095647253875104	25.33421932156109
52	26.361220032106107	25.758572594015632	22.07360720018948	25.806600173688782
53	27.53296139372089	24.121358456801495	20.441985315403034	27.90369483407458
54	27.305323824311166	24.409194978815233	21.01798731545567	27.26749388141793
55	27.271112397694676	24.340114213531937	29.17182031106082	19.21695307771257
56	26.386549646043317	24.625319087344405	30.691926103318508	18.29620516329377
57	32.67454406694913	26.19674201952683	23.6966762283218	17.432037685202243
58	25.662846390694494	26.294113002973763	30.54981710045001	17.493223505881733
59	25.81581094239322	26.312534540382643	21.909129187610198	25.96252532961394
60	25.560212110845022	24.543409037079922	30.23270349219716	19.663675359877892
61	25.869430774494067	25.86844390641859	22.060777915208295	26.201347403879048
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.5
2	2.5
3	2.0
4	2.0
5	2.0
6	1.5
7	3.5
8	6.0
9	6.5
10	7.0
11	13.0
12	15.5
13	12.0
14	16.0
15	20.0
16	21.5
17	30.0
18	37.0
19	58.0
20	178.0
21	277.0
22	359.5
23	442.0
24	508.5
25	653.5
26	732.0
27	1028.0
28	1324.0
29	1849.0
30	3311.5
31	4249.0
32	4808.5
33	5368.0
34	6501.0
35	8510.0
36	9386.0
37	10582.5
38	11779.0
39	12885.5
40	14895.0
41	15798.0
42	17141.5
43	18541.5
44	18598.0
45	18730.0
46	18862.0
47	20152.0
48	23879.0
49	26316.0
50	26081.5
51	25847.0
52	22231.5
53	16424.5
54	14233.0
55	13160.0
56	12087.0
57	10904.5
58	8853.5
59	7985.0
60	7496.5
61	6581.0
62	6154.0
63	5600.5
64	5047.0
65	4685.0
66	4021.0
67	3719.0
68	3410.0
69	3101.0
70	2777.5
71	2140.0
72	1826.0
73	1617.0
74	1408.0
75	1167.5
76	748.0
77	569.0
78	471.0
79	373.0
80	309.5
81	194.5
82	143.0
83	106.5
84	51.5
85	33.0
86	30.5
87	28.0
88	20.5
89	12.5
90	12.0
91	11.5
92	11.0
93	12.5
94	16.0
95	18.0
96	15.0
97	12.0
98	19.5
99	74.5
100	122.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013158241006342272
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	3.289560251585568E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	303992.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.34003517505583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.43237028031324	41.57493054509654
2	8.78435295986441	9.195466858188182
3	3.3544620590656953	5.267179864946661
4	1.8586048336067862	3.8911776947003185
5	1.3576969105275267	3.5530952027037688
6	1.0042474864480386	3.153740925909104
7	0.7629666177932174	2.795358972888326
8	0.6588095252529309	2.7585690980320194
9	0.45319053077089655	2.134800748939585
>10	2.2700499082649315	18.900294412237933
>50	0.042804803052392065	1.4254994145845585
>100	0.01533306377996134	1.7441719320837574
>500	0.0025555106299935564	0.9399668905459053
>1k	0.0025555106299935564	2.66574743914335
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCCACCCCCCTTGTCTC	3652	1.2013474038790495	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATGCTTTTTTCCACCCCCCTTGTCTC	2198	0.7230453432985079	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCCACCCCCCTTGTCCC	1121	0.3687597042027422	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCGACCCCCCTTGTCTC	1001	0.32928498118371535	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCCACCCCCCTTTTCTC	812	0.2671122924287481	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCAGTGCTTTTTTCCACCCCCCTTGTCTC	712	0.23421668991289246	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATGCTTTTTTCCACCCCCCTTGTCCC	676	0.2223742730071844	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCCACCCCCCTTTTCCC	611	0.20099213137187819	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATGCTTTTTTCGACCCCCCTTGTCTC	481	0.15822784810126583	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATGCTTTTTTCCACCCCCCTTTTCTC	477	0.15691202400063162	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTTCTTTTTTCCACCCCCCTTGTCTC	439	0.14441169504460644	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCAATGCTTTTTTCCACCCCCCTTGTCTC	388	0.12763493776152005	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATGCTTTTTTCCACCCCCCTTTTCCC	352	0.115792520855812	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCCACCCCCGTTGTCTC	337	0.11085818047843364	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATTCTTTTTTCCACCCCCCTTGTCTC	328	0.10789757625200662	Illumina Single End PCR Primer 1 (96% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.300218426800706	0.0	0.0	0.0	0.0
2	8.33114029316561	0.0	0.0	0.0	0.0
3	8.345614358272586	0.0	0.0	0.0	0.0
4	8.359430511329245	0.0	0.0	0.0	0.0
5	8.370286060159478	0.0	0.0	0.0	0.0
6	8.405813310876603	0.0	0.0	0.0	0.0
7	8.443972209794994	0.0	0.0	0.0	3.289560251585568E-4
8	8.549567093870891	0.0	0.0	0.0	3.289560251585568E-4
9	8.681478459959473	0.0	0.0	0.0	3.289560251585568E-4
10	8.873917734677228	0.0	0.0	0.0	3.289560251585568E-4
11	9.079186294376168	0.0	0.0	0.0	3.289560251585568E-4
12	9.332811389773415	0.0	0.0	0.0	3.289560251585568E-4
13	9.633477196768336	0.0	0.0	0.0	3.289560251585568E-4
14	9.957827837574673	0.0	0.0	0.0	3.289560251585568E-4
15	10.346324903286929	0.0	0.0	0.0	3.289560251585568E-4
16	10.77002026369115	0.0	0.0	0.0	3.289560251585568E-4
17	11.128582331113977	0.0	0.0	0.0	3.289560251585568E-4
18	11.531553461933209	0.0	0.0	0.0	3.289560251585568E-4
19	11.899984210110793	0.0	0.0	0.0	3.289560251585568E-4
20	12.280257375194084	0.0	0.0	0.0	3.289560251585568E-4
21	12.695070922919024	0.0	0.0	0.0	3.289560251585568E-4
22	13.09014710913445	0.0	0.0	0.0	3.289560251585568E-4
23	13.529303402721125	0.0	0.0	0.0	3.289560251585568E-4
24	14.033592989289192	0.0	0.0	0.0	3.289560251585568E-4
25	14.514855654096161	0.0	0.0	0.0	3.289560251585568E-4
26	15.078357325192767	0.0	0.0	0.0	3.289560251585568E-4
27	15.664228006000158	0.0	0.0	0.0	3.289560251585568E-4
28	16.321482144266955	0.0	0.0	0.0	6.579120503171136E-4
29	17.03761941103713	0.0	0.0	0.0	6.579120503171136E-4
30	17.7975078291534	0.0	0.0	0.0	6.579120503171136E-4
31	18.676478328377062	0.0	0.0	0.0	6.579120503171136E-4
32	19.56827811258191	0.0	0.0	0.0	6.579120503171136E-4
33	20.492644543277454	0.0	0.0	0.0	6.579120503171136E-4
34	21.44595910418695	0.0	0.0	0.0	6.579120503171136E-4
35	22.46342009000237	0.0	0.0	0.0	6.579120503171136E-4
36	23.5180531066607	0.0	0.0	0.0	6.579120503171136E-4
37	24.61906892286639	0.0	0.0	0.0	6.579120503171136E-4
38	25.785546988078632	0.0	0.0	0.0	6.579120503171136E-4
39	26.950380273165084	0.0	0.0	0.0	6.579120503171136E-4
40	28.109950261848997	0.0	0.0	0.0	6.579120503171136E-4
41	29.299455248822337	0.0	0.0	0.0	6.579120503171136E-4
42	30.603436932550856	0.0	0.0	0.0	6.579120503171136E-4
43	31.921234769336035	0.0	0.0	0.0	6.579120503171136E-4
44	33.13409563409564	0.0	0.0	0.0	6.579120503171136E-4
45	34.41998473644043	0.0	0.0	0.0	6.579120503171136E-4
46	35.743374825653305	0.0	0.0	0.0	6.579120503171136E-4
47	37.03748782862707	0.0	0.0	0.0	6.579120503171136E-4
48	38.32995605147504	0.0	0.0	0.0	6.579120503171136E-4
49	39.65170136056212	0.0	0.0	0.0	6.579120503171136E-4
50	40.952722440064214	0.0	0.0	0.0	6.579120503171136E-4
>>END_MODULE