FastQCFastQC Report
Sat 10 Aug 2024
AM-AL-LC3_S57_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-LC3_S57_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303992
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCCACCCCCCTTGTCTC36521.2013474038790495Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATGCTTTTTTCCACCCCCCTTGTCTC21980.7230453432985079Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCCACCCCCCTTGTCCC11210.3687597042027422Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCGACCCCCCTTGTCTC10010.32928498118371535Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCCACCCCCCTTTTCTC8120.2671122924287481Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCAGTGCTTTTTTCCACCCCCCTTGTCTC7120.23421668991289246Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATGCTTTTTTCCACCCCCCTTGTCCC6760.2223742730071844Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCCACCCCCCTTTTCCC6110.20099213137187819Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATGCTTTTTTCGACCCCCCTTGTCTC4810.15822784810126583Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATGCTTTTTTCCACCCCCCTTTTCTC4770.15691202400063162Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTTCTTTTTTCCACCCCCCTTGTCTC4390.14441169504460644Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCAATGCTTTTTTCCACCCCCCTTGTCTC3880.12763493776152005Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATGCTTTTTTCCACCCCCCTTTTCCC3520.115792520855812Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCGTGCTTTTTTCCACCCCCGTTGTCTC3370.11085818047843364Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCATTCTTTTTTCCACCCCCCTTGTCTC3280.10789757625200662Illumina Single End PCR Primer 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)