##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-LC3_S57_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	303992
Sequences flagged as poor quality	0
Sequence length	61
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.46710439748414	40.0	40.0	40.0	40.0	40.0
2	39.191386615437246	40.0	40.0	40.0	40.0	40.0
3	39.17743230085002	40.0	40.0	40.0	40.0	40.0
4	39.0684557488355	40.0	40.0	40.0	40.0	40.0
5	39.185886470696595	40.0	40.0	40.0	40.0	40.0
6	39.18158372588752	40.0	40.0	40.0	40.0	40.0
7	39.25965156977815	40.0	40.0	40.0	40.0	40.0
8	39.23209821311087	40.0	40.0	40.0	40.0	40.0
9	39.24784862759547	40.0	40.0	40.0	40.0	40.0
10	39.2435721992684	40.0	40.0	40.0	40.0	40.0
11	39.26133582462696	40.0	40.0	40.0	40.0	40.0
12	39.27829679728414	40.0	40.0	40.0	40.0	40.0
13	39.14008263375352	40.0	40.0	40.0	40.0	40.0
14	39.26746756493592	40.0	40.0	40.0	40.0	40.0
15	39.23811152925077	40.0	40.0	40.0	40.0	40.0
16	39.379575778309956	40.0	40.0	40.0	40.0	40.0
17	39.34300902655333	40.0	40.0	40.0	40.0	40.0
18	39.397497302560595	40.0	40.0	40.0	40.0	40.0
19	39.36582541645833	40.0	40.0	40.0	40.0	40.0
20	39.353719834732495	40.0	40.0	40.0	40.0	40.0
21	39.3705360667386	40.0	40.0	40.0	40.0	40.0
22	39.39698413116135	40.0	40.0	40.0	40.0	40.0
23	39.389628674438804	40.0	40.0	40.0	40.0	40.0
24	39.40211584515382	40.0	40.0	40.0	40.0	40.0
25	39.38285218032053	40.0	40.0	40.0	40.0	40.0
26	39.335969367614936	40.0	40.0	40.0	40.0	40.0
27	39.35708834443012	40.0	40.0	40.0	40.0	40.0
28	39.34261427932314	40.0	40.0	40.0	40.0	40.0
29	39.34574594068265	40.0	40.0	40.0	40.0	40.0
30	39.352206637016764	40.0	40.0	40.0	40.0	40.0
31	39.335719361035814	40.0	40.0	40.0	40.0	40.0
32	39.347706518592595	40.0	40.0	40.0	40.0	40.0
33	39.343022184794336	40.0	40.0	40.0	40.0	40.0
34	39.34670649227611	40.0	40.0	40.0	40.0	40.0
35	39.345509092344535	40.0	40.0	40.0	40.0	40.0
36	39.311916103055346	40.0	40.0	40.0	40.0	40.0
37	39.32686386483855	40.0	40.0	40.0	40.0	40.0
38	39.304389589199715	40.0	40.0	40.0	40.0	40.0
39	38.09349588147057	40.0	40.0	40.0	40.0	40.0
40	39.27320455801468	40.0	40.0	40.0	40.0	40.0
41	39.30765283296929	40.0	40.0	40.0	40.0	40.0
42	39.14912234532488	40.0	40.0	40.0	40.0	40.0
43	39.28656017263612	40.0	40.0	40.0	40.0	40.0
44	39.27105976473065	40.0	40.0	40.0	40.0	40.0
45	38.880615279349456	40.0	40.0	40.0	40.0	40.0
46	39.035606200163166	40.0	40.0	40.0	40.0	40.0
47	38.84218005736993	40.0	40.0	40.0	40.0	40.0
48	39.23121661096344	40.0	40.0	40.0	40.0	40.0
49	39.20662385852259	40.0	40.0	40.0	40.0	40.0
50	39.16775441458986	40.0	40.0	40.0	40.0	40.0
51	39.098410484486436	40.0	40.0	40.0	40.0	40.0
52	39.098410484486436	40.0	40.0	40.0	40.0	40.0
53	38.882931129766575	40.0	40.0	40.0	40.0	40.0
54	38.66337272033475	40.0	40.0	40.0	40.0	40.0
55	38.728637595726205	40.0	40.0	40.0	40.0	40.0
56	38.67567567567568	40.0	40.0	40.0	40.0	40.0
57	38.396273586147004	40.0	40.0	40.0	40.0	40.0
58	37.941551093449824	40.0	40.0	40.0	24.0	40.0
59	37.387431248190744	40.0	40.0	40.0	24.0	40.0
60	37.300981604779075	40.0	40.0	40.0	24.0	40.0
61	36.9159714729335	40.0	40.0	40.0	24.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.06749443981458825
1101	2	-0.013046370249149675
1101	3	-0.08702616825001286
1101	4	-0.07515661359503412
1101	5	-0.0356569617861382
1101	6	-0.032329740064263035
1101	7	-0.16511877970364708
1101	8	-0.02247628784386535
1101	9	-0.13054390278787054
1101	10	-0.03630744761291993
1101	11	0.053979412405126936
1101	12	-0.16527610279845817
1101	13	-0.00574738977647371
1101	14	-0.05530800819445858
1101	15	0.07973370390228496
1101	16	0.12821734517191175
1101	17	-0.29471705370050927
1101	18	-0.03739916551728584
1101	19	-0.03953283017839482
1101	20	-0.05045429033762616
1101	21	0.06990473452630397
1101	22	0.09688443949262648
1101	23	0.018890399902780075
1101	24	0.08220913080995729
1101	25	-0.022805043779570155
1101	26	-0.21290531052641626
1101	27	0.11746317054009836
1101	28	-0.016496964731622654
1101	29	0.04662467394136627
1101	30	0.12814946257017112
1101	31	-0.012454310813794223
1101	32	0.02462349619714388
1101	33	-0.10130112604192476
1101	34	-0.08930210265568661
1101	35	-0.054801007941144064
1101	36	-0.24238914418895519
1101	37	-0.07861797051962327
1101	38	0.14043747190267908
1101	39	1.3844896907342132
1101	40	-0.3576372514285211
1101	41	-0.028630234078050876
1101	42	-0.17581777524696207
1101	43	-0.06073604348412687
1101	44	-0.1671987414464553
1101	45	0.0583408035688322
1101	46	0.20023882203980747
1101	47	0.03457316950544964
1101	48	0.04092322861897202
1101	49	0.07136787145390144
1101	50	0.1282991370793951
1101	51	0.11898380959303267
1101	52	0.13630832969128903
1101	53	0.022348132051135394
1101	54	-0.020002658225806158
1101	55	0.30759084792141067
1101	56	0.0382203559842651
1101	57	-0.141820346214395
1101	58	-0.09128951823176124
1101	59	0.02539759551541465
1101	60	-0.1658277653079594
1101	61	-0.17383658294418325
1102	1	0.027050149623086384
1102	2	0.1283975823113792
1102	3	0.10857002398979887
1102	4	-0.0750858851888978
1102	5	0.02330480951034275
1102	6	0.08857725529659177
1102	7	0.12949718112567865
1102	8	0.04858647075686662
1102	9	-0.23634073865617466
1102	10	-0.08095636144964402
1102	11	0.09968282247878335
1102	12	-0.14741396532173212
1102	13	-0.2315639010013939
1102	14	-0.04644766786222476
1102	15	0.026057273500640576
1102	16	-0.20308736887636059
1102	17	-0.2102673367748764
1102	18	-0.09120419302099236
1102	19	0.10900968241607956
1102	20	-0.22789257176595612
1102	21	0.003175698265138749
1102	22	0.112328951450543
1102	23	-0.006378930652857662
1102	24	-0.03026189465548157
1102	25	-0.043689213154813444
1102	26	-0.11674039760273303
1102	27	-0.1434860689687838
1102	28	0.058025055914647794
1102	29	-0.20963077134217656
1102	30	-0.15754185937588971
1102	31	-0.36762664700520276
1102	32	-0.12009968246602654
1102	33	0.0849074777471941
1102	34	-0.1549252038390989
1102	35	-0.11637330040952776
1102	36	-0.07991956544091039
1102	37	-0.19780176471282118
1102	38	-0.29778287280185367
1102	39	1.0863373095981217
1102	40	-0.17329330561933887
1102	41	0.09976111262295007
1102	42	-0.09505879515057813
1102	43	-0.05579791476485951
1102	44	0.028191695430045627
1102	45	-0.1744199511675646
1102	46	-0.10852282001658864
1102	47	-0.1893401046089167
1102	48	-0.2023053302254496
1102	49	-0.22544709982071964
1102	50	-0.11085289362959116
1102	51	-0.04129961841991303
1102	52	-0.044949285668344885
1102	53	0.0786993821757207
1102	54	-0.46143793048194226
1102	55	-0.053599832646604284
1102	56	-0.33752751653742763
1102	57	-0.18862969136580432
1102	58	-0.2098303269146342
1102	59	-0.2800976801485504
1102	60	0.030089919687007693
1102	61	0.1523885455725491
1103	1	0.20935239460368393
1103	2	0.030975861170212227
1103	3	0.1677389677340244
1103	4	-0.11007795128738707
1103	5	-0.1027576052653103
1103	6	0.11893545527342297
1103	7	0.13077255712246227
1103	8	0.19428967588302726
1103	9	0.1529890406293859
1103	10	0.2256015156444917
1103	11	-0.05959888575982575
1103	12	0.023269604328149285
1103	13	0.034326486808559764
1103	14	0.21463455243984697
1103	15	0.10992599904929534
1103	16	-0.016010966414462757
1103	17	-0.035421790122157404
1103	18	0.05441398703871414
1103	19	0.12653334861192178
1103	20	0.09518567573988435
1103	21	0.1486408332052349
1103	22	0.05152327517711086
1103	23	0.0813881941926411
1103	24	-0.11962323949695985
1103	25	0.15949943939981637
1103	26	0.02078339613071023
1103	27	0.010549592669711672
1103	28	0.10875101056014813
1103	29	0.08464523490291498
1103	30	0.16133189859388608
1103	31	0.11639980004171946
1103	32	9.547558955844693E-4
1103	33	0.007776988714894628
1103	34	0.15568411767149826
1103	35	0.11181272168210654
1103	36	0.1811371473412251
1103	37	0.08823531259303508
1103	38	0.1409379675859057
1103	39	1.326941242645134
1103	40	-0.03545491914002241
1103	41	0.030980085116283362
1103	42	0.030506222986332432
1103	43	0.08177689400845622
1103	44	0.08729261912111497
1103	45	-0.1799635855002606
1103	46	0.11621543563040149
1103	47	0.1430909002066869
1103	48	0.1209939824531645
1103	49	0.0032405701629869554
1103	50	0.12179269319747732
1103	51	0.12953699634957871
1103	52	0.05578841306446236
1103	53	-0.07156966901893469
1103	54	0.10255034031013821
1103	55	-0.1990947263599523
1103	56	0.06554656474015985
1103	57	0.0746461756507415
1103	58	-0.11856867183794861
1103	59	-0.08860847673776817
1103	60	-0.11660807871664502
1103	61	-0.16269734568677308
1104	1	-0.1297601768466805
1104	2	-0.2640279259036049
1104	3	-0.18924641252633023
1104	4	-0.45209376436395843
1104	5	-0.28475946883257564
1104	6	0.010552127116667975
1104	7	-0.1571278782547978
1104	8	-0.12679315477755182
1104	9	-0.31302919181521105
1104	10	0.024850639713392297
1104	11	-0.02735372313361495
1104	12	0.10146968170433013
1104	13	0.15980668231965467
1104	14	0.22219780202163264
1104	15	-0.11916853612144962
1104	16	-0.11823471436426303
1104	17	-0.01270152682516823
1104	18	0.009596829142260788
1104	19	0.07071787446483313
1104	20	-0.1649377992136607
1104	21	-0.01688753170521551
1104	22	-0.005652276128202516
1104	23	0.04159721887128143
1104	24	-0.10937470937201255
1104	25	0.08799948571654426
1104	26	-0.0685152595943137
1104	27	-0.019489803796048477
1104	28	-0.05526470443393805
1104	29	-0.0793704800911712
1104	30	0.08423906351958976
1104	31	-0.028871261810188287
1104	32	0.22008411034934738
1104	33	0.04546245997399723
1104	34	-0.1667980231422561
1104	35	-0.07703311989288153
1104	36	-0.28080085362869767
1104	37	-0.11410232002265985
1104	38	-0.03108999682105207
1104	39	1.1461076504830174
1104	40	-0.310957728113479
1104	41	-0.07649652493499559
1104	42	-0.030596987035522716
1104	43	0.025660633566758406
1104	44	0.11639914215142255
1104	45	-0.038341192178585004
1104	46	0.16380505850324312
1104	47	-0.03853171107606812
1104	48	-0.18017156443843163
1104	49	0.09553837039075574
1104	50	0.05974353207519556
1104	51	-0.22604907375760774
1104	52	-0.021452635546310717
1104	53	0.11566761976315121
1104	54	0.08502626923302614
1104	55	-0.22787866698735826
1104	56	-0.008024019232067303
1104	57	-0.10364599028302734
1104	58	0.15061326572709532
1104	59	0.09320158107640708
1104	60	0.17964026614138362
1104	61	0.31681267779853783
1201	1	0.014498724540210617
1201	2	0.05179608898388466
1201	3	0.031762347088047704
1201	4	0.12461149746096822
1201	5	-0.0124637451320595
1201	6	0.22810769368368256
1201	7	-0.1337219243597687
1201	8	0.10487029103781254
1201	9	0.075670850578895
1201	10	-0.11287186054813247
1201	11	-0.08486436295434885
1201	12	0.007700514886259668
1201	13	-0.07830507296171874
1201	14	-0.04441160506644337
1201	15	0.007590957131277776
1201	16	0.16048729588740684
1201	17	0.04040893460995676
1201	18	0.05699454646713775
1201	19	0.009049308321756655
1201	20	-0.039376352528385894
1201	21	0.05625937765397282
1201	22	-0.14139711863683146
1201	23	-0.12384772659447663
1201	24	0.035377505307366164
1201	25	-0.17811660807904417
1201	26	0.19320506039863972
1201	27	0.08723764611558238
1201	28	-0.005470893974155899
1201	29	-0.008765015104856388
1201	30	0.12288927906886471
1201	31	0.044487067531491675
1201	32	-0.0016558100378176732
1201	33	0.03451707129708126
1201	34	0.12560902639278027
1201	35	-0.010519512484371774
1201	36	0.15947245074450223
1201	37	0.07382361691472283
1201	38	0.13094580142702483
1201	39	1.279359499031429
1201	40	0.022997752564165808
1201	41	0.0322746514740615
1201	42	0.1092132855531105
1201	43	-0.06655812004638051
1201	44	-0.023367084607514244
1201	45	-0.07709271313614607
1201	46	0.15278479191585603
1201	47	0.3110115887273821
1201	48	0.22673261936481026
1201	49	0.21552801962078405
1201	50	-0.03134764829798087
1201	51	0.11319631106530181
1201	52	0.10565922434390984
1201	53	0.22401155162425113
1201	54	0.1935772164936509
1201	55	0.09739097607624814
1201	56	-0.026954916831023468
1201	57	0.19996191374110595
1201	58	0.24962829086074834
1201	59	0.2987326361391993
1201	60	0.18178089346410786
1201	61	0.5142965597777334
1202	1	0.13771589752594338
1202	2	0.2324508188509995
1202	3	0.13707758276318316
1202	4	0.1379379153228939
1202	5	0.2599188413629818
1202	6	0.13782817163409078
1202	7	-0.020377782884011708
1202	8	0.035141462329477235
1202	9	0.022556550667083286
1202	10	0.03397969711831195
1202	11	-0.10932947407488314
1202	12	0.017264742326979388
1202	13	-0.32464373316524586
1202	14	-0.0015835252079696716
1202	15	0.050488626639157985
1202	16	-0.13820452743752298
1202	17	0.1756781650028003
1202	18	0.07559672962491959
1202	19	-0.05618663864657947
1202	20	0.1292089225192683
1202	21	0.02461783393808048
1202	22	0.07706653762509319
1202	23	-0.014039609179341994
1202	24	-0.012895964617406719
1202	25	-0.1939265065542699
1202	26	0.0951575937337168
1202	27	0.09328324690803669
1202	28	0.0877748868815118
1202	29	0.2141393306600179
1202	30	0.06632594215818699
1202	31	-0.0791606914099603
1202	32	0.10010600416434556
1202	33	0.08633091463556042
1202	34	0.06491815340150708
1202	35	0.07441900397303414
1202	36	-0.13103389006862898
1202	37	-0.1711058528882674
1202	38	-0.18491726788970198
1202	39	0.9423890191781794
1202	40	-0.10960097503259192
1202	41	-0.17117068857467643
1202	42	-0.14891647087224413
1202	43	-0.3541373462312265
1202	44	-0.13097442936008008
1202	45	-0.4416119954493922
1202	46	-0.059750696087000676
1202	47	0.031409076106101
1202	48	-0.04299686174648798
1202	49	0.004088568596131381
1202	50	-0.060062077385588
1202	51	0.05975146031878609
1202	52	0.0230780572602427
1202	53	-0.1475623813978828
1202	54	0.0884098588235176
1202	55	-0.03724324876573348
1202	56	-0.09542678249690084
1202	57	-0.3243091074978679
1202	58	-0.3725162229196286
1202	59	3.452675304416175E-4
1202	60	0.003613993563384099
1202	61	-0.06528083532946027
1203	1	-0.1551483106537077
1203	2	-0.01995070112823072
1203	3	0.026057304214759824
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2403	13	-0.0966193289450672
2403	14	0.0035939447081005937
2403	15	-0.1072893758551956
2403	16	0.046161940354970454
2403	17	0.057890724412246186
2403	18	-0.11615841919363135
2403	19	-0.024110594557186005
2403	20	0.13197293640675412
2403	21	-0.1089851592867106
2403	22	0.12235591639611698
2403	23	0.028216095812737763
2403	24	0.004160023517563616
2403	25	-0.014953236591011887
2403	26	0.023104470735098914
2403	27	-0.05164546175816298
2403	28	0.03171259544658511
2403	29	-0.03970500816763689
2403	30	-0.006697956449691844
2403	31	-0.1426790788438126
2403	32	-0.22558921274332988
2403	33	-0.10172989366996887
2403	34	-0.04227165345459838
2403	35	0.09589741432797894
2403	36	0.04806072684578311
2403	37	0.1570702350208606
2403	38	0.0732281462067732
2403	39	1.3791166963952861
2403	40	0.13649064191553606
2403	41	-0.1192341934791159
2403	42	0.04095105870607085
2403	43	0.057201852204748604
2403	44	-0.030998024600577878
2403	45	0.03307031149098094
2403	46	0.021255135308152262
2403	47	0.010213808637196564
2403	48	0.17456473868496403
2403	49	0.03682527941960245
2403	50	0.12029994101870045
2403	51	0.12138222851240243
2403	52	-0.06582880895474119
2403	53	0.2800642744823847
2403	54	-0.06018622436656074
2403	55	0.10432819425176376
2403	56	0.21945507276315368
2403	57	0.049008046241972636
2403	58	0.12350966442994604
2403	59	0.2664051994413157
2403	60	0.3092869635300133
2403	61	0.1545324251957041
2404	1	-0.07436859174263333
2404	2	-0.14961373992392168
2404	3	0.03424024163125239
2404	4	0.05021703571041769
2404	5	-0.01066849058823749
2404	6	-0.02022698609056306
2404	7	-0.03512033899486511
2404	8	0.19963313458803356
2404	9	-0.06444322565736371
2404	10	-0.08629766057228494
2404	11	0.014419862409937423
2404	12	-0.11547167772904032
2404	13	0.06588711236174305
2404	14	-0.08762183048170868
2404	15	0.12664514611395816
2404	16	-0.15704668927847365
2404	17	-0.017110134428662604
2404	18	-0.15609108606642508
2404	19	-0.1841023496844656
2404	20	-0.08905835975594556
2404	21	-0.38308595285570846
2404	22	-0.1415212350003614
2404	23	-0.021637901232850254
2404	24	-0.12233006545835678
2404	25	-0.15385185901019582
2404	26	0.0035641223892213247
2404	27	-0.1387250591335416
2404	28	-0.15371459202120263
2404	29	0.005353221245947282
2404	30	-0.34539732777814436
2404	31	-0.10548096142308339
2404	32	-0.07660436269695481
2404	33	-0.2266946811927255
2404	34	0.08785992097255502
2404	35	-0.1286516941951632
2404	36	-0.11643822536830584
2404	37	0.008662526064867393
2404	38	-0.25728131822003775
2404	39	1.166852448224617
2404	40	-0.03656923647259447
2404	41	-0.06020090480550522
2404	42	-0.0661498379238381
2404	43	0.080754705274245
2404	44	-0.09620122635949002
2404	45	0.0010252395201106879
2404	46	-0.17807840414699427
2404	47	-0.10186149453364379
2404	48	0.039596172262207574
2404	49	-0.04955350377459666
2404	50	0.02494798801447473
2404	51	-0.14378661961016803
2404	52	-0.06544404822781758
2404	53	0.07089185358163519
2404	54	0.2984245145801552
2404	55	-0.00766466019187817
2404	56	-0.14026872400073387
2404	57	0.3368090699803403
2404	58	0.16799075275314834
2404	59	0.3364728761048923
2404	60	0.11598592261483276
2404	61	0.2644503200048689
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	6.0
18	24.0
19	25.0
20	50.0
21	72.0
22	102.0
23	164.0
24	199.0
25	311.0
26	394.0
27	517.0
28	751.0
29	865.0
30	1103.0
31	1321.0
32	1871.0
33	2267.0
34	2984.0
35	3961.0
36	5980.0
37	11820.0
38	34248.0
39	120260.0
40	114696.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.248585489091816	27.405655411984526	28.925761204242217	22.41999789468144
2	32.02445750541343	26.664341141394132	18.53728848141006	22.773912871782382
3	19.346954350622223	33.08124253678562	25.046465191403694	22.525337921188456
4	21.471688306561706	24.990213526058337	32.78748383998211	20.75061432739785
5	22.572962446380167	23.826284902234267	23.474301955314615	30.12645069607095
6	30.85081186347009	24.844403800100004	23.537790468170215	20.766993868259693
7	30.449485512776654	24.810192373483513	23.352588226005945	21.387733887733887
8	21.518329429721835	34.3051790836601	22.320324219058396	21.856167267559673
9	22.22065054343535	32.833429827100716	23.590423432195585	21.35549619726835
10	29.80078423116398	25.582252164530644	23.257190978709964	21.35977262559541
11	21.454511960841074	32.30019211031869	23.938787862838495	22.306508066001737
12	29.54551435564094	24.258862075317772	23.46377536250954	22.73184820653175
13	22.145319613674044	24.232545593305087	23.15554356693597	30.466591226084898
14	22.36243059027869	32.63342456380431	22.507500197373613	22.49664464854338
15	21.502210584489063	24.775323034816708	23.282520592647174	30.43994578804705
16	21.469643937998367	33.51502671122925	23.30554751440827	21.709781836364115
17	22.372957183083766	24.41380036316745	22.58184425905945	30.631398194689336
18	30.182044264322744	24.97664412221374	23.16508329166557	21.676228321797943
19	22.169662359535778	24.049974999342087	31.674517750467118	22.105844890655018
20	21.80682386378589	24.35689097081502	22.700597384141687	31.135687781257403
21	22.1821626884918	24.297020974236165	31.700834232479803	21.81998210479223
22	30.01263191136609	24.764138529961315	23.451275033553515	21.77195452511908
23	22.289073396668332	32.999223663780626	22.30782389010237	22.40387904944867
24	22.261770046580175	33.163043764309585	22.82066633332456	21.754519855785677
25	22.026237532566647	24.946380167899154	22.808823916418852	30.218558383115347
26	22.046303850101317	24.748348640753704	30.93995894628806	22.265388562856916
27	22.020974236164108	24.781902155319877	22.68118897865733	30.51593462985868
28	21.668991289244456	25.466130687649674	22.855206715966208	30.009671307139662
29	22.147622305850152	33.348574962499015	22.43578778388905	22.06801494776178
30	30.5396194636701	25.013158241006344	22.714413537198347	21.732808758125213
31	22.072291376088845	25.591791889260247	31.100818442590594	21.235098292060318
32	22.297955209347613	25.167438616805704	22.051567146503857	30.483039027342823
33	22.310784494328797	33.85944366957025	22.236440432642965	21.593331403457984
34	21.731163977999422	25.70758441011606	22.614081949524987	29.947169662359535
35	22.34400905286981	33.91372141372141	22.121963735887785	21.620305797520984
36	30.61856890970815	25.976341482670595	21.935445669622887	21.469643937998367
37	30.368233374562486	26.415826732282426	22.34828548119687	20.86765441195821
38	22.249927629674467	26.21318982078476	29.90243164293797	21.634450906602805
39	22.67407711712201	26.182610791779698	21.51837277915658	29.624939311941713
40	22.438748388115474	34.635450932919284	21.41240558962078	21.513395089344456
41	22.649609200242114	34.53248769704466	21.00219742624806	21.815705676465168
42	22.649609200242114	26.39016816232006	21.007131766625438	29.95309087081239
43	22.800928971815047	34.90453696149899	21.08246269638675	21.212071370299217
44	22.850930287639148	26.774717755730414	28.603384299586832	21.770967657043606
45	22.561777941524777	26.788204952761912	20.85614095107766	29.793876154635647
46	22.44927498092055	27.287560198952605	28.54713281928472	21.716032000842127
47	31.225163820100526	26.677675728308643	20.019934735124608	22.077225716466224
48	31.130424484854863	27.131963999052605	19.93045869628148	21.807152819811048
49	30.82811389773415	27.17472828232322	20.165991210295008	21.831166609647624
50	31.2491776099371	27.369470249217088	19.52025053290876	21.86110160793705
51	30.973512460854234	27.06847548619701	19.98967078081002	21.96834127213874
52	31.028448117055714	27.645135398299953	19.48439432616648	21.842022158477857
53	31.091278717860998	27.124726966499118	19.340969499197346	22.443024816442538
54	30.686004894865654	27.687899681570567	19.463012184531173	22.163083239032606
55	30.73238769441301	27.699413142451117	19.53143503776415	22.03676412537172
56	31.067264928024425	27.40532645595937	19.29919208400221	22.228216532014
57	30.95805152767178	27.136898339429983	19.329127082291638	22.575923050606594
58	30.492578752072426	27.294139319455773	19.665978052054	22.5473038764178
59	30.9991710308166	26.72340061580568	19.379786310166057	22.897642043211665
60	31.473525619095238	27.036566751756624	19.066949130240268	22.422958498907867
61	31.4547751256612	26.713202979025763	19.31794257743625	22.514079317876785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	1.5
3	3.0
4	3.5
5	4.0
6	5.5
7	5.0
8	3.0
9	7.0
10	11.0
11	8.5
12	7.0
13	8.0
14	16.0
15	24.0
16	23.5
17	42.0
18	61.0
19	84.5
20	159.0
21	210.0
22	301.5
23	393.0
24	482.5
25	785.0
26	998.0
27	1313.0
28	1628.0
29	2145.0
30	3456.0
31	4250.0
32	4924.0
33	5598.0
34	6735.5
35	8805.5
36	9738.0
37	10653.0
38	11568.0
39	12643.5
40	15265.0
41	16811.0
42	17835.0
43	18904.5
44	18950.0
45	18334.0
46	17718.0
47	17797.0
48	17881.5
49	17887.0
50	17265.0
51	16643.0
52	16110.5
53	14347.5
54	13117.0
55	12382.0
56	11647.0
57	10789.5
58	9521.0
59	9110.0
60	9641.0
61	18513.5
62	26855.0
63	16198.0
64	5541.0
65	4793.5
66	3744.5
67	3443.0
68	3124.0
69	2805.0
70	2517.0
71	2022.0
72	1815.0
73	1515.5
74	1216.0
75	1067.0
76	738.0
77	558.0
78	445.0
79	332.0
80	280.0
81	163.5
82	99.0
83	87.0
84	53.5
85	32.0
86	25.5
87	19.0
88	13.0
89	5.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0388168109687097
3	3.289560251585568E-4
4	3.289560251585568E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1096213058238376
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	303992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.08885954666913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42905175513327	34.57690304742569
2	12.021701338205272	11.321764116535046
3	4.332958819838731	6.121022678666617
4	2.3909560335379956	4.503495713821272
5	1.7422123998477952	4.101939749844908
6	1.174212482332564	3.317539599510228
7	0.9302544032829023	3.0663233257201368
8	0.7592796613911242	2.860289066551132
9	0.588609176689	2.494524136408999
>10	2.596654882441021	19.055223966570384
>50	0.02830325201517473	0.7880417134674842
>100	0.004354346463873036	0.5112360812434329
>500	7.25724410645506E-4	0.2511754810921946
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	7.25724410645506E-4	7.030521323142476
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCAACATCTGGGGGGGGGGGGGGG	20573	6.76761230558699	TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCAACATCTGGGGGGGGGGGGGGT	735	0.24178267849153923	TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAGCTCCAGTCACAGGTCAACATCTGGGGGGGGGGGGGGG	463	0.15230663964841182	TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCAACATCTGGGGGGGGGGGGGTG	345	0.11348982867970209	TruSeq Adapter, Index 15 (97% over 39bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.421603200084213	0.0	0.0	0.0	0.0
2	8.460420011052923	0.0	0.0	0.0	0.0
3	8.48377588883918	0.0	0.0	0.0	0.0
4	8.506802810600279	0.0	0.0	3.289560251585568E-4	0.0
5	8.535421984789073	0.0	0.0	3.289560251585568E-4	0.0
6	8.600226321745309	0.0	0.0	3.289560251585568E-4	0.0
7	8.687728624437485	0.0	0.0	3.289560251585568E-4	0.0
8	9.379194189320772	0.0	0.0	3.289560251585568E-4	0.0
9	9.542027421774257	0.0	0.0	3.289560251585568E-4	0.0
10	9.732492960341062	0.0	0.0	3.289560251585568E-4	0.0
11	9.918353114555646	0.0	0.0	3.289560251585568E-4	0.0
12	10.170991341877418	0.0	0.0	3.289560251585568E-4	0.0
13	10.450603963262191	0.0	0.0	3.289560251585568E-4	0.0
14	10.759493670886076	0.0	0.0	3.289560251585568E-4	0.0
15	11.13220084739072	0.0	0.0	3.289560251585568E-4	0.0
16	11.521684781178452	0.0	0.0	3.289560251585568E-4	0.0
17	11.84800915813574	0.0	0.0	3.289560251585568E-4	0.0
18	12.152622437432564	0.0	0.0	3.289560251585568E-4	0.0
19	12.510526592805073	0.0	0.0	3.289560251585568E-4	0.0
20	12.820074212479275	0.0	0.0	3.289560251585568E-4	0.0
21	13.164162214795127	0.0	0.0	3.289560251585568E-4	0.0
22	13.542461643727467	0.0	0.0	3.289560251585568E-4	0.0
23	13.912537172030843	0.0	0.0	3.289560251585568E-4	0.0
24	14.333271928208637	0.0	0.0	3.289560251585568E-4	0.0
25	14.796113055606726	0.0	0.0	3.289560251585568E-4	0.0
26	15.321126871759784	0.0	0.0	3.289560251585568E-4	0.0
27	15.881010026579647	0.0	0.0	3.289560251585568E-4	0.0
28	16.47970999236822	0.0	0.0	3.289560251585568E-4	0.0
29	17.1267664938551	0.0	0.0	3.289560251585568E-4	0.0
30	17.81954788283902	0.0	0.0	3.289560251585568E-4	0.0
31	18.63831942945867	0.0	0.0	3.289560251585568E-4	0.0
32	19.461696360430537	0.0	0.0	3.289560251585568E-4	0.0
33	20.34560120003158	0.0	0.0	3.289560251585568E-4	0.0
34	21.254177741519513	0.0	0.0	3.289560251585568E-4	0.0
35	22.22394010368694	0.0	0.0	3.289560251585568E-4	0.0
36	23.234164056948867	0.0	0.0	3.289560251585568E-4	0.0
37	24.303929050764495	0.0	0.0	3.289560251585568E-4	0.0
38	25.443103765888576	0.0	0.0	3.289560251585568E-4	0.0
39	26.575699360509486	0.0	0.0	3.289560251585568E-4	0.0
40	27.74250638174689	0.0	0.0	3.289560251585568E-4	0.0
41	28.93595884102213	0.0	0.0	3.289560251585568E-4	9.868680754756703E-4
42	30.253427721782153	0.0	0.0	3.289560251585568E-4	9.868680754756703E-4
43	31.574186162793758	0.0	0.0	3.289560251585568E-4	9.868680754756703E-4
44	32.80349482881128	0.0	0.0	3.289560251585568E-4	9.868680754756703E-4
45	34.10155530408695	0.0	0.0	3.289560251585568E-4	9.868680754756703E-4
46	35.43876154635648	0.0	0.0	3.289560251585568E-4	9.868680754756703E-4
47	36.747019658412064	0.0	0.0	3.289560251585568E-4	9.868680754756703E-4
48	38.04639595778836	0.0	0.0	3.289560251585568E-4	9.868680754756703E-4
49	39.36912813495092	0.0	0.0	3.289560251585568E-4	9.868680754756703E-4
50	40.685610147635465	0.0	0.0	3.289560251585568E-4	9.868680754756703E-4
>>END_MODULE