FastQCFastQC Report
Sat 10 Aug 2024
AM-AL-LC3_S57_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-LC3_S57_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303992
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCAACATCTGGGGGGGGGGGGGGG205736.76761230558699TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCAACATCTGGGGGGGGGGGGGGT7350.24178267849153923TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAGCTCCAGTCACAGGTCAACATCTGGGGGGGGGGGGGGG4630.15230663964841182TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCAACATCTGGGGGGGGGGGGGTG3450.11348982867970209TruSeq Adapter, Index 15 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)