##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-LC3.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81619 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.52814908293412 40.0 40.0 40.0 40.0 40.0 2 39.269704358053886 40.0 40.0 40.0 40.0 40.0 3 39.26835663264681 40.0 40.0 40.0 40.0 40.0 4 39.14421887060611 40.0 40.0 40.0 40.0 40.0 5 39.25056665727343 40.0 40.0 40.0 40.0 40.0 6 39.313052107965056 40.0 40.0 40.0 40.0 40.0 7 39.378821107830284 40.0 40.0 40.0 40.0 40.0 8 39.36750021441086 40.0 40.0 40.0 40.0 40.0 9 39.362403361962286 40.0 40.0 40.0 40.0 40.0 10-11 39.3661769931021 40.0 40.0 40.0 40.0 40.0 12-13 39.32018280057339 40.0 40.0 40.0 40.0 40.0 14-15 39.346892267731775 40.0 40.0 40.0 40.0 40.0 16-17 39.44155159950502 40.0 40.0 40.0 40.0 40.0 18-19 39.4814197674561 40.0 40.0 40.0 40.0 40.0 20-21 39.458434923240915 40.0 40.0 40.0 40.0 40.0 22-23 39.48311055023953 40.0 40.0 40.0 40.0 40.0 24-25 39.46532057486614 40.0 40.0 40.0 40.0 40.0 26-27 39.43620970607334 40.0 40.0 40.0 40.0 40.0 28-29 39.448584275720115 40.0 40.0 40.0 40.0 40.0 30-31 39.42996764747145 40.0 40.0 40.0 40.0 40.0 32-33 39.43896959578903 40.0 40.0 40.0 40.0 40.0 34-35 39.443422699528696 40.0 40.0 40.0 40.0 40.0 36-37 39.421356640293894 40.0 40.0 40.0 40.0 40.0 38-39 39.328374226684986 40.0 40.0 40.0 40.0 40.0 40-41 39.39238802889418 40.0 40.0 40.0 40.0 40.0 42-43 39.38632299546062 40.0 40.0 40.0 40.0 40.0 44-45 39.292646537227824 40.0 40.0 40.0 40.0 40.0 46-47 39.26816841739899 40.0 40.0 40.0 40.0 40.0 48-49 39.351418444517876 40.0 40.0 40.0 40.0 40.0 50-51 39.30442248946903 40.0 40.0 40.0 40.0 40.0 52-53 39.23717114610439 40.0 40.0 40.0 40.0 40.0 54-55 39.208571857852206 40.0 40.0 40.0 40.0 40.0 56-57 39.188414370129735 40.0 40.0 40.0 40.0 40.0 58-59 39.13476950807552 40.0 40.0 40.0 40.0 40.0 60-61 39.04466333247726 40.0 40.0 40.0 40.0 40.0 62-63 39.32566063629528 40.0 40.0 40.0 40.0 40.0 64-65 39.379897392845194 40.0 40.0 40.0 40.0 40.0 66-67 39.33578670212778 40.0 40.0 40.0 40.0 40.0 68-69 39.30510496218757 40.0 40.0 40.0 40.0 40.0 70-71 39.32597951168112 40.0 40.0 40.0 40.0 40.0 72-73 39.32725144696579 40.0 40.0 40.0 40.0 40.0 74-75 39.313335751748966 40.0 40.0 40.0 40.0 40.0 76-77 39.3372049304715 40.0 40.0 40.0 40.0 40.0 78-79 39.332253799593246 40.0 40.0 40.0 40.0 40.0 80-81 39.32933647886746 40.0 40.0 40.0 40.0 40.0 82-83 39.357046698096426 40.0 40.0 40.0 40.0 40.0 84-85 39.32172923583595 40.0 40.0 40.0 40.0 40.0 86-87 39.302862655922226 40.0 40.0 40.0 40.0 40.0 88-89 39.42441419592841 40.0 40.0 40.0 40.0 40.0 90-91 39.414577455268564 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.05560199578908254 1101 2 -0.38119893333796284 1101 3 -0.7100177711764815 1101 4 0.13347421965377038 1101 5 0.3377979683345913 1101 6 0.15361900327652478 1101 7 0.13088917882453188 1101 8 -0.1197316514980713 1101 9 0.17865717490277433 1101 10-11 0.03671737382303775 1101 12-13 0.38685849308435394 1101 14-15 0.11136572403044198 1101 16-17 0.08745513149565909 1101 18-19 0.1326151953017245 1101 20-21 -0.024680407566343376 1101 22-23 0.08144813471133716 1101 24-25 0.2157858220061044 1101 26-27 0.26438027109553985 1101 28-29 -0.002434822524548963 1101 30-31 0.012610483508652237 1101 32-33 0.004974965074723059 1101 34-35 0.24459678376072702 1101 36-37 -0.13251648491588952 1101 38-39 0.13704041624886543 1101 40-41 0.07149085141890055 1101 42-43 -0.04836609511877299 1101 44-45 0.21011859204587324 1101 46-47 0.4941961701140656 1101 48-49 0.02649081758949734 1101 50-51 0.4022720798356687 1101 52-53 0.033621301448128804 1101 54-55 0.2655150152083081 1101 56-57 0.11318195110393958 1101 58-59 0.5662478479706223 1101 60-61 -0.3422646361963899 1101 62-63 0.16529786638357535 1101 64-65 0.28801750141548865 1101 66-67 -0.05697230603121284 1101 68-69 0.6691270165805747 1101 70-71 0.41321395458854937 1101 72-73 0.6696119156814007 1101 74-75 0.21280022230961748 1101 76-77 0.07571596609042075 1101 78-79 0.3753488076214495 1101 80-81 0.3153472458433342 1101 82-83 0.4703176471244461 1101 84-85 0.7569023573908638 1101 86-87 0.751862629329743 1101 88-89 0.7766341754006163 1101 90-91 1.8839285714285765 1102 1 -0.09184429836890473 1102 2 0.1072040723859331 1102 3 -0.031613951628429504 1102 4 -0.0668350335269281 1102 5 -0.5913722022972081 1102 6 -0.38090070483188043 1102 7 -0.44529832626187016 1102 8 -0.258081758651592 1102 9 -0.21013375367059695 1102 10-11 -0.009670603281378476 1102 12-13 0.15155480103541663 1102 14-15 -0.1733642219858922 1102 16-17 0.09212105667808856 1102 18-19 -0.11229162183199293 1102 20-21 -0.1741527782361274 1102 22-23 0.13760200173246773 1102 24-25 -0.07100581188153399 1102 26-27 -0.02273689245598831 1102 28-29 0.07542102208928725 1102 30-31 -0.25128556401867996 1102 32-33 -0.1748588654776171 1102 34-35 0.04887395145986062 1102 36-37 0.0068658156001646375 1102 38-39 -0.210079954055864 1102 40-41 -0.06643088719635415 1102 42-43 0.08309160470285093 1102 44-45 -0.2930122719079975 1102 46-47 -0.23155393524369572 1102 48-49 -0.16883754218995506 1102 50-51 -0.006890491778008823 1102 52-53 0.055915527432183865 1102 54-55 -0.3433671310294244 1102 56-57 -0.10136744969333478 1102 58-59 -0.14486326314049336 1102 60-61 0.15758926297684184 1102 62-63 -0.3441245763966876 1102 64-65 -0.12032538202559095 1102 66-67 -0.22633048408504663 1102 68-69 -0.023180675727118683 1102 70-71 -0.030578513520026718 1102 72-73 -0.0749768289073387 1102 74-75 -0.9542129447035492 1102 76-77 -0.213721099058219 1102 78-79 0.07978230515839613 1102 80-81 0.6789836094796939 1102 82-83 0.8703176471244447 1102 84-85 0.7569023573908638 1102 86-87 0.751862629329743 1102 88-89 0.7766341754006163 1102 90-91 1.8839285714285765 1103 1 -0.10743964028651476 1103 2 0.1556317130774687 1103 3 0.04307952808223803 1103 4 -0.16666574198637107 1103 5 0.4352527399897639 1103 6 0.25563548764747424 1103 7 -0.3820961511220702 1103 8 -0.02497243735676591 1103 9 0.34350088440461235 1103 10-11 -0.08328677318852584 1103 12-13 -0.06252204706390074 1103 14-15 0.10027246810798829 1103 16-17 -0.22465932602339223 1103 18-19 0.04366179734412157 1103 20-21 -0.10222952373451477 1103 22-23 -0.26207993537315843 1103 24-25 0.1542545379876472 1103 26-27 0.02292052302649239 1103 28-29 -0.09330621332855316 1103 30-31 -0.35434820188868343 1103 32-33 0.3578365401544801 1103 34-35 0.051987286443171854 1103 36-37 -0.14404858593286463 1103 38-39 0.1596935958120369 1103 40-41 0.003575923209858445 1103 42-43 0.20064202112921947 1103 44-45 -0.43207977489720406 1103 46-47 -0.029112080005987195 1103 48-49 0.013480157976125895 1103 50-51 0.03950327040522694 1103 52-53 -0.18986354703671537 1103 54-55 -0.7395439043618524 1103 56-57 0.18720325963473528 1103 58-59 0.06122192955727712 1103 60-61 -0.6915170098390178 1103 62-63 0.3271351802858291 1103 64-65 -0.3329644062537298 1103 66-67 0.32585530826468556 1103 68-69 -0.008817030263926995 1103 70-71 -0.11265127715903134 1103 72-73 0.16530352565872874 1103 74-75 0.22418002316310748 1103 76-77 0.010409483136875508 1103 78-79 0.4087866341627233 1103 80-81 0.6789836094796939 1103 82-83 0.8703176471244447 1103 84-85 -0.29071669022818725 1103 86-87 0.751862629329743 1103 88-89 0.7766341754006163 1103 90-91 1.8839285714285765 1104 1 0.21400609652017266 1104 2 0.14774937144524358 1104 3 0.44527120356046623 1104 4 -0.07015512005039426 1104 5 -0.3174147597699175 1104 6 0.12586522906821784 1104 7 -0.24790409113937528 1104 8 0.21880339653730374 1104 9 0.11664324718457664 1104 10-11 -0.04166416432568809 1104 12-13 -0.18402545954106841 1104 14-15 -0.005850765574599848 1104 16-17 0.18820234849357576 1104 18-19 0.02337918655877047 1104 20-21 -0.2060457305979142 1104 22-23 0.014859409046721339 1104 24-25 -0.2071796636849399 1104 26-27 -0.21912504075318395 1104 28-29 -0.12041377845399381 1104 30-31 -0.1620959669564499 1104 32-33 0.16408722362363903 1104 34-35 0.2888253193163095 1104 36-37 -0.14736722456259344 1104 38-39 0.03976128025254155 1104 40-41 0.16675106587838684 1104 42-43 0.2290473261302708 1104 44-45 -0.39478325910944534 1104 46-47 -0.059414740687749656 1104 48-49 0.016868072920281918 1104 50-51 -0.22575966793642976 1104 52-53 0.2415360957031325 1104 54-55 0.3431791466883354 1104 56-57 0.03336878181708158 1104 58-59 -0.19396236223958851 1104 60-61 -0.5719153507786174 1104 62-63 -0.11059422645767114 1104 64-65 0.19451257923542897 1104 66-67 0.4051160567954568 1104 68-69 0.5058617104581273 1104 70-71 0.27317089321941523 1104 72-73 0.6696119156814007 1104 74-75 0.4028084123178104 1104 76-77 0.21610039572434658 1104 78-79 0.33121087658696524 1104 80-81 -0.262192861108538 1104 82-83 -0.2011109243041247 1104 84-85 0.02962963011813713 1104 86-87 0.751862629329743 1104 88-89 0.7766341754006163 1104 90-91 1.8839285714285765 1201 1 0.20939204288180235 1201 2 0.1952356734735048 1201 3 -0.13513829369993857 1201 4 0.24917584217204336 1201 5 0.4352527399897639 1201 6 0.037813705469254444 1201 7 0.07334939343238034 1201 8 0.21265132501947193 1201 9 0.04647118143431328 1201 10-11 -0.12289073358456193 1201 12-13 -0.022918086667864657 1201 14-15 0.021064547315916116 1201 16-17 0.07237037694690684 1201 18-19 -0.2533679056261775 1201 20-21 -0.013120612843415813 1201 22-23 -0.034357163095933174 1201 24-25 -0.21208209567571856 1201 26-27 0.11202943391758424 1201 28-29 -0.07350423313053511 1201 30-31 -0.007813548423335703 1201 32-33 -0.21753419849709132 1201 34-35 -0.03628042528900011 1201 36-37 0.17433929785963898 1201 38-39 -0.1571864913816583 1201 40-41 0.18593640586446014 1201 42-43 0.3034237433638296 1201 44-45 0.19346195141238098 1201 46-47 0.06336154562312402 1201 48-49 0.20869604908903483 1201 50-51 0.018540441408191555 1201 52-53 0.39417685700368565 1201 54-55 -0.20234056657331223 1201 56-57 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0.1871649891192959 1202 24-25 -0.005061393605508613 1202 26-27 0.12323055402959682 1202 28-29 0.19752286957974263 1202 30-31 -0.00891365843433789 1202 32-33 0.1262472325377928 1202 34-35 0.15646878695977762 1202 36-37 -0.23702425188540843 1202 38-39 -0.42074351450521164 1202 40-41 -0.04270179919758732 1202 42-43 -0.10967149473140836 1202 44-45 0.06275323424793555 1202 46-47 -0.32309662261481265 1202 48-49 -0.24905274171092628 1202 50-51 0.12629465765879644 1202 52-53 -0.14704530176273778 1202 54-55 -0.160365108635105 1202 56-57 -0.2499759436328972 1202 58-59 -0.21450654213883524 1202 60-61 -0.3773776713094321 1202 62-63 0.05823793073065531 1202 64-65 -0.794057561464534 1202 66-67 -0.1890835205222956 1202 68-69 0.3135714610250204 1202 70-71 -1.0846723932977937 1202 72-73 -0.17935469799109427 1202 74-75 -0.8164608936450435 1202 76-77 -0.6518104290830351 1202 78-79 -2.257880032503941 1202 80-81 -0.4789111273624158 1202 82-83 -1.412510635703839 1202 84-85 0.7569023573908638 1202 86-87 0.751862629329743 1202 88-89 0.7766341754006163 1202 90-91 -38.11607142857142 1203 1 -0.07725603940215109 1203 2 -0.08526033729261684 1203 3 -0.35084530129390146 1203 4 -0.1284075472348576 1203 5 0.4398667936281342 1203 6 -0.5731638971453705 1203 7 0.1404454234237278 1203 8 -0.20838106948211532 1203 9 -0.679473257669791 1203 10-11 -0.26496513519948195 1203 12-13 -0.2228604109973773 1203 14-15 -0.005850765574599848 1203 16-17 -0.3069432825743874 1203 18-19 -0.09312566781015619 1203 20-21 -0.11866708982121565 1203 22-23 -0.2181502996911462 1203 24-25 -0.29455830446163844 1203 26-27 -0.18999882716094874 1203 28-29 -0.20779241923069947 1203 30-31 0.022370052461027967 1203 32-33 -0.3132321109822982 1203 34-35 -0.4980625050197105 1203 36-37 -0.02098060560920345 1203 38-39 -0.1929371271678022 1203 40-41 -0.15596037457157763 1203 42-43 -0.6682554546311579 1203 44-45 -0.2792431100817936 1203 46-47 -0.4003823756560507 1203 48-49 0.052844833704433825 1203 50-51 -0.34055301917796044 1203 52-53 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0.22446423635650348 2401 74-75 -0.13723995871647077 2401 76-77 0.1559421631523179 2401 78-79 -0.15976682495676897 2401 80-81 0.5050705660014287 2401 82-83 0.4541014309082314 2401 84-85 -0.6177874689118639 2401 86-87 -1.4995993589743506 2401 88-89 0.7766341754006163 2401 90-91 1.8839285714285765 2402 1 0.12529998756367178 2402 2 0.0204685989108242 2402 3 0.3377202522082712 2402 4 0.42829616707316376 2402 5 0.029444866977264894 2402 6 0.2400274925316097 2402 7 0.024773490265658893 2402 8 0.025461560466609967 2402 9 -0.047442231354686726 2402 10-11 0.04017034464121849 2402 12-13 0.1921165535253877 2402 14-15 0.03975167071993724 2402 16-17 -0.025285769364778332 2402 18-19 0.2042011756787403 2402 20-21 0.23488193540067925 2402 22-23 0.12496118160717629 2402 24-25 0.20259154326995343 2402 26-27 0.04819061035694716 2402 28-29 -0.05771042854900088 2402 30-31 0.047982379694943234 2402 32-33 0.028839812443237633 2402 34-35 0.15751775027845127 2402 36-37 -0.10809077191335348 2402 38-39 -0.3688276578594909 2402 40-41 -0.013339663789736278 2402 42-43 0.07517409908631123 2402 44-45 0.29561643870903254 2402 46-47 0.14863477506433753 2402 48-49 0.07248036105587374 2402 50-51 0.2597994631979219 2402 52-53 0.29726106228520166 2402 54-55 0.09043665238696974 2402 56-57 0.5554294617725049 2402 58-59 0.18037600757104144 2402 60-61 0.2953053993280079 2402 62-63 0.036001158107644926 2402 64-65 0.026805525554316034 2402 66-67 0.032331179454672565 2402 68-69 0.012302124461569974 2402 70-71 -0.010517694949548684 2402 72-73 -0.6456590202791901 2402 74-75 0.4283828378922365 2402 76-77 -0.24672643261972382 2402 78-79 -0.4148268592620923 2402 80-81 -0.631671076014527 2402 82-83 -0.046963270156474834 2402 84-85 0.47118807167657906 2402 86-87 -0.010042132575016183 2402 88-89 0.7766341754006163 2402 90-91 1.8839285714285765 2403 1 -0.3163429733679166 2403 2 0.01686669153714604 2403 3 0.11472944116515293 2403 4 -0.193824183021313 2403 5 0.16397064994870192 2403 6 0.17552060593029495 2403 7 -0.06153585081723634 2403 8 -0.30512258977658746 2403 9 0.33463282202585276 2403 10-11 -0.03005320555694624 2403 12-13 -0.24106738251347082 2403 14-15 0.008971734981244595 2403 16-17 0.07970164442480154 2403 18-19 -0.01997662756707541 2403 20-21 0.0881314971003988 2403 22-23 -0.22235000818196227 2403 24-25 -0.009225168761652469 2403 26-27 -0.037845858955215306 2403 28-29 0.06790609110800006 2403 30-31 0.20058591747745425 2403 32-33 -0.2014481055140891 2403 34-35 -0.030921731128806584 2403 36-37 0.11172514021594537 2403 38-39 0.12692707151274618 2403 40-41 0.09848620330876656 2403 42-43 0.010314408090501104 2403 44-45 -0.034057246770530014 2403 46-47 0.039406967270082305 2403 48-49 0.0013454315352774415 2403 50-51 0.2041694299712873 2403 52-53 -0.14126251191944306 2403 54-55 0.1261347955104526 2403 56-57 0.25768388316574686 2403 58-59 0.22078243867542824 2403 60-61 -0.20487604439604468 2403 62-63 -0.018238614589854762 2403 64-65 0.036263219330059826 2403 66-67 0.005543318067402936 2403 68-69 0.27218879897532133 2403 70-71 0.03792690283091105 2403 72-73 0.3539189030811727 2403 74-75 0.43218150835757285 2403 76-77 -0.1444314585510753 2403 78-79 0.20343904057983764 2403 80-81 0.08015320012297167 2403 82-83 0.6278934047002025 2403 84-85 0.37594997643848416 2403 86-87 0.30741818488530015 2403 88-89 0.7766341754006163 2403 90-91 -0.7827380952380949 2404 1 0.18655068897896854 2404 2 0.16364460739015385 2404 3 0.11409035009467061 2404 4 0.06874756678636373 2404 5 -0.04340256740144355 2404 6 -0.05078864313770026 2404 7 0.10088796124265542 2404 8 0.24111091516453342 2404 9 0.05568140478223427 2404 10-11 -0.007164277217995618 2404 12-13 9.824429199838391E-4 2404 14-15 0.3046933753149901 2404 16-17 0.20748435346083483 2404 18-19 0.1642236209683432 2404 20-21 0.22565442745131037 2404 22-23 0.3002963022506009 2404 24-25 0.2520210588257825 2404 26-27 0.3064111976753523 2404 28-29 0.13390999666454206 2404 30-31 0.10013026921424029 2404 32-33 0.004149395707472081 2404 34-35 0.2854449582753915 2404 36-37 0.3203877448582091 2404 38-39 0.35823426788653023 2404 40-41 0.3346355221906947 2404 42-43 0.08013300273523072 2404 44-45 0.37125346278934046 2404 46-47 0.30337033959115445 2404 48-49 0.2331988726039853 2404 50-51 0.07406188917437362 2404 52-53 0.29965066713783983 2404 54-55 0.3660373922331388 2404 56-57 0.15646759239162833 2404 58-59 0.03622714403687155 2404 60-61 -0.18415576975419867 2404 62-63 0.3512933744841291 2404 64-65 0.40166043899730397 2404 66-67 0.2605742778197211 2404 68-69 0.20915841375482813 2404 70-71 0.4788925363961596 2404 72-73 0.4744899644618883 2404 74-75 0.6452326547420526 2404 76-77 0.45114613815925253 2404 78-79 0.023656492935025142 2404 80-81 0.6789836094796939 2404 82-83 0.8703176471244447 2404 84-85 0.7569023573908638 2404 86-87 0.751862629329743 2404 88-89 -0.023365824599380858 2404 90-91 1.8839285714285765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 1.0 31 67.0 32 410.0 33 684.0 34 855.0 35 1143.0 36 1556.0 37 2219.0 38 5702.0 39 32536.0 40 36446.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 24.483269826878544 19.440326394589494 29.907251987894973 26.169151790636985 2 26.79976468858004 26.96031570948844 19.066352918106723 27.17356668382479 3 22.377142577096016 24.923118391550986 26.243889290483835 26.45584974086916 4 24.956198924270083 25.770960193092296 25.10444871904826 24.16839216358936 5 26.35783334762739 24.310515933789926 23.985836631176564 25.345814087406122 6 25.853048922432276 25.563900562369057 24.153689704603096 24.429360810595572 7 25.03338683394798 25.18531224347272 24.333794827184846 25.447506095394456 8 25.135078841936316 25.453632119972063 23.42346757495191 25.98782146313971 9 25.055440522427375 24.956198924270083 24.3533981058332 25.634962447469338 10-11 24.922505789093226 24.78528283855475 24.072826180178637 26.219385192173387 12-13 24.76751736727968 24.681140420735368 24.111420135017582 26.43992207696737 14-15 25.094034477266327 24.20331050368174 23.720579766966026 26.982075252085913 16-17 24.953748514439038 24.366262757446183 23.749372082480793 26.930616645633982 18-19 24.92005537926218 24.344821671424548 23.915387348534043 26.819735600779232 20-21 25.315183964518067 23.836361631482866 23.57723079184994 27.271223612149132 22-23 24.845317879415333 23.933152819809113 24.055673311361325 27.16585598941423 24-25 24.80917433440743 24.366875359903943 24.14388806527892 26.680062240409708 26-27 25.10016050184393 24.18003161028682 23.888432840392557 26.83137504747669 28-29 25.359291341476865 24.17390558570921 23.928864602604786 26.537938470209145 30-31 25.148101180521092 24.283692635680286 24.276953802233617 26.291252381565002 32-33 25.386961639223927 24.09350360067331 23.918848797033398 26.60068596306937 34-35 24.96570008285004 24.176304888152444 24.203479701739848 26.654515327257666 36-37 25.006616243923503 24.549050743108662 24.535121808532864 25.909211204434975 38-39 25.45032299789078 24.07602025442974 24.097332970772182 26.376323776907306 40-41 25.095284300407528 24.421502037647972 24.19018047739181 26.293033184552687 42-43 25.147133978469803 24.428361825945036 24.361595146639413 26.06290904894575 44-45 25.260693018075305 24.114433052980537 24.488918902326724 26.135955026617435 46-47 25.075763244537562 24.52951960490871 24.22178988326848 26.172927267285246 48-49 24.70190460947503 24.653889244558258 24.742917733674776 25.901288412291933 50-51 25.13047400184704 24.35192543115483 24.38843667447005 26.12916389252808 52-53 24.984138336774834 24.325446722115196 24.57692618270329 26.11348875840668 54-55 24.761679273261972 24.976012162145324 24.742987451557028 25.519321113035677 56-57 25.2636263113892 24.316998840313925 24.499042584751475 25.920332263545404 58-59 24.697315318476925 24.091945955430777 24.97660408258759 26.23413464350471 60-61 25.423404763801482 24.512068828168566 24.8578029156377 25.206723492392257 62-63 24.960824598669777 24.31660688790612 24.685726224884217 26.03684228853989 64-65 25.0187265917603 24.154422356669546 25.057140113319885 25.769710938250263 66-67 25.23334814908883 24.647067538679703 24.87988655364362 25.239697758587848 68-69 25.45544414630702 23.81725744951278 24.994115708704044 25.73318269547616 70-71 25.178335535006607 24.348745046235138 25.01981505944518 25.453104359313077 72-73 25.53229772686379 24.185809392430876 25.073472080609367 25.208420800095965 74-75 25.068119891008173 24.516262546131827 25.278515503742284 25.137102059117716 76-77 25.11327639440234 24.592004490957937 24.776454549099803 25.518264565539916 78-79 25.619873668332232 24.04072782125012 25.794286791741307 24.545111718676345 80-81 25.087206031281646 23.669404748509056 25.531675481039724 25.71171373916957 82-83 25.778522552716097 23.47437508727831 25.631894986733695 25.115207373271893 84-85 25.364352312845117 23.327600253462478 26.414411152349054 24.89363628134335 86-87 27.328957334006564 22.077758141883365 25.296642262055038 25.296642262055038 88-89 26.414708886619 22.288049029622066 26.06741573033708 25.229826353421856 90-91 24.951267056530213 22.904483430799218 24.269005847953213 27.87524366471735 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 1.5 3 1.5 4 1.5 5 1.0 6 0.5 7 0.0 8 0.0 9 0.0 10 2.5 11 7.5 12 10.5 13 13.0 14 15.5 15 20.5 16 34.0 17 50.0 18 63.0 19 84.5 20 115.5 21 156.0 22 189.5 23 242.5 24 347.5 25 455.5 26 553.5 27 671.5 28 828.0 29 1030.0 30 1278.5 31 1566.5 32 1874.0 33 2148.0 34 2425.5 35 2731.0 36 3022.5 37 3271.0 38 3491.5 39 3752.0 40 4055.0 41 4274.0 42 4361.0 43 4457.5 44 4536.5 45 4523.5 46 4460.5 47 4357.5 48 4233.5 49 4116.0 50 4033.0 51 3966.5 52 3897.5 53 3854.5 54 3799.5 55 3738.0 56 3669.5 57 3591.0 58 3526.0 59 3469.0 60 3382.5 61 3365.0 62 3399.5 63 3343.0 64 3321.0 65 3314.5 66 3148.5 67 2994.0 68 2893.0 69 2728.5 70 2556.0 71 2334.5 72 2092.0 73 1827.0 74 1579.0 75 1358.5 76 1122.5 77 882.0 78 683.5 79 541.5 80 411.5 81 305.0 82 228.5 83 161.5 84 103.0 85 73.5 86 54.0 87 36.0 88 21.5 89 12.0 90 8.0 91 7.5 92 6.5 93 4.0 94 1.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.03063012288805303 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.10718349667804573 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.002116491703352523 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0034489894460922946 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 1726.0 32-33 3552.0 34-35 3628.0 36-37 3668.0 38-39 3723.0 40-41 3714.0 42-43 3713.0 44-45 3556.0 46-47 3480.0 48-49 3498.0 50-51 3220.0 52-53 3217.0 54-55 3125.0 56-57 2904.0 58-59 2833.0 60-61 2680.0 62-63 2711.0 64-65 2464.0 66-67 2377.0 68-69 2335.0 70-71 2253.0 72-73 2222.0 74-75 2044.0 76-77 1921.0 78-79 1727.0 80-81 1767.0 82-83 1638.0 84-85 1602.0 86-87 1506.0 88-89 1421.0 90-91 1394.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.37146987833715 #Duplication Level Percentage of deduplicated Percentage of total 1 99.44763642640496 98.82257807618325 2 0.4845510813010135 0.9630110636003872 3 0.05794885705126625 0.17275389308861908 4 0.0073977264320765415 0.029404917972530906 5 0.0024659088106921806 0.012252049155221212 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.004900819662088485 0.0 0.0 0.0 0.0 2 0.006126024577610605 0.0 0.0 0.0 0.0 3 0.006126024577610605 0.0 0.0 0.0 0.0 4 0.006126024577610605 0.0 0.0 0.0 0.0 5 0.007351229493132726 0.0 0.0 0.0 0.0 6 0.01225204915522121 0.0 0.0 0.0 0.0 7 0.025729303225964544 0.0 0.0 0.0 0.0 8 0.24749139293546846 0.0 0.0 0.0 0.0 9 0.25729303225964545 0.0 0.0 0.0 0.0 10-11 0.25729303225964545 0.0 0.0 0.0 0.0 12-13 0.25729303225964545 0.0 0.0 0.0 0.0 14-15 0.25729303225964545 0.0 0.0 0.0 0.0 16-17 0.25729303225964545 0.0 0.0 0.0 0.0 18-19 0.25729303225964545 0.0 0.0 0.0 0.0 20-21 0.25729303225964545 0.0 0.0 0.0 0.0 22-23 0.25729303225964545 0.0 0.0 0.0 0.0 24-25 0.25729303225964545 0.0 0.0 0.0 0.0 26-27 0.25729303225964545 0.0 0.0 0.0 0.0 28-29 0.25851823717516753 0.0 0.0 0.0 0.0 30-31 0.2609686470062118 0.0 0.0 0.0 0.0 32-33 0.2609686470062118 0.0 0.0 0.0 0.0 34-35 0.26464426175277816 0.0 0.0 0.0 0.0 36-37 0.26464426175277816 0.0 0.0 0.0 0.0 38-39 0.26586946666830025 0.0 0.0 0.0 0.0 40-41 0.26770727404158345 0.0 0.0 0.0 6.126024577610606E-4 42-43 0.2719954912459109 0.0 0.0 0.0 0.0012252049155221211 44-45 0.2719954912459109 0.0 0.0 0.0 0.0012252049155221211 46-47 0.2719954912459109 0.0 0.0 0.0 0.0012252049155221211 48-49 0.2719954912459109 0.0 0.0 0.0 0.0012252049155221211 50-51 0.2744459010769551 0.0 0.0 0.0 0.0012252049155221211 52-53 0.2744459010769551 0.0 0.0 0.0 0.0012252049155221211 54-55 0.2744459010769551 0.0 0.0 0.0 0.0012252049155221211 56-57 0.27505850353471617 0.0 0.0 0.0 0.0012252049155221211 58-59 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 60-61 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 62-63 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 64-65 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 66-67 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 68-69 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 70-71 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 72-73 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 74-75 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 76-77 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 78-79 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 80 0.2768963109079994 0.0 0.0 0.0 0.0012252049155221211 >>END_MODULE