##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-LC2_S109_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45307
Sequences flagged as poor quality	0
Sequence length	61
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.51866157547399	40.0	40.0	40.0	40.0	40.0
2	39.49915024168451	40.0	40.0	40.0	40.0	40.0
3	39.230935617012825	40.0	40.0	40.0	40.0	40.0
4	39.49005672412652	40.0	40.0	40.0	40.0	40.0
5	39.12402057077273	40.0	40.0	40.0	40.0	40.0
6	39.36654380117863	40.0	40.0	40.0	40.0	40.0
7	39.16798728673273	40.0	40.0	40.0	40.0	40.0
8	38.96934248570861	40.0	40.0	40.0	40.0	40.0
9	39.24188315271371	40.0	40.0	40.0	40.0	40.0
10	39.41510141920674	40.0	40.0	40.0	40.0	40.0
11	39.376785044253644	40.0	40.0	40.0	40.0	40.0
12	39.39576665857373	40.0	40.0	40.0	40.0	40.0
13	38.38011786258194	40.0	40.0	40.0	40.0	40.0
14	39.36133489306288	40.0	40.0	40.0	40.0	40.0
15	39.452711501533976	40.0	40.0	40.0	40.0	40.0
16	39.31357185423886	40.0	40.0	40.0	40.0	40.0
17	39.38084622685236	40.0	40.0	40.0	40.0	40.0
18	39.3110115434701	40.0	40.0	40.0	40.0	40.0
19	39.31118811662657	40.0	40.0	40.0	40.0	40.0
20	23.971571721808992	24.0	12.0	40.0	12.0	40.0
21	25.53300814443684	24.0	12.0	40.0	12.0	40.0
22	26.05478182179354	24.0	12.0	40.0	12.0	40.0
23	36.203412276248706	40.0	40.0	40.0	24.0	40.0
24	38.27258481029421	40.0	40.0	40.0	40.0	40.0
25	26.152426777319178	24.0	12.0	40.0	12.0	40.0
26	37.84174630851745	40.0	40.0	40.0	24.0	40.0
27	38.117730152073634	40.0	40.0	40.0	40.0	40.0
28	22.4955084203324	12.0	12.0	40.0	12.0	40.0
29	36.0235725163882	40.0	40.0	40.0	24.0	40.0
30	20.63063102831792	12.0	12.0	40.0	12.0	40.0
31	29.263734080826364	24.0	24.0	40.0	12.0	40.0
32	21.206524378131416	12.0	12.0	40.0	12.0	40.0
33	33.399695411305096	40.0	24.0	40.0	24.0	40.0
34	36.78698655836846	40.0	40.0	40.0	24.0	40.0
35	19.681373739157305	12.0	12.0	24.0	12.0	40.0
36	22.280707175491646	24.0	12.0	24.0	12.0	40.0
37	20.262299423930077	12.0	12.0	24.0	12.0	40.0
38	33.52938839472929	40.0	24.0	40.0	24.0	40.0
39	19.51000949080716	12.0	12.0	24.0	12.0	40.0
40	23.75500474540358	24.0	12.0	40.0	12.0	40.0
41	19.382082238947625	12.0	12.0	24.0	12.0	40.0
42	21.683713333480476	24.0	12.0	24.0	12.0	40.0
43	28.190345862670227	24.0	24.0	40.0	12.0	40.0
44	18.241154788443286	12.0	12.0	24.0	12.0	40.0
45	27.905091928399585	24.0	24.0	40.0	12.0	40.0
46	20.149998896417774	12.0	12.0	24.0	12.0	40.0
47	17.51614540799435	12.0	12.0	24.0	12.0	40.0
48	24.592049793630125	24.0	24.0	24.0	12.0	40.0
49	17.70304809411349	12.0	12.0	24.0	12.0	40.0
50	24.290462842386386	24.0	24.0	24.0	12.0	40.0
51	16.99322400512062	12.0	12.0	12.0	12.0	40.0
52	24.732248879864038	24.0	24.0	24.0	12.0	40.0
53	16.602997329331007	12.0	12.0	12.0	12.0	40.0
54	17.229302315315515	12.0	12.0	24.0	12.0	40.0
55	22.696536958968814	24.0	12.0	24.0	12.0	40.0
56	16.505793806696538	12.0	12.0	12.0	12.0	40.0
57	21.04019246474055	24.0	12.0	24.0	12.0	40.0
58	22.523671838788708	24.0	12.0	24.0	12.0	40.0
59	18.997417617586688	12.0	12.0	24.0	12.0	24.0
60	15.991789348224335	12.0	12.0	12.0	12.0	24.0
61	21.318118613017855	24.0	12.0	24.0	12.0	24.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.24798756620491957
1101	2	0.17282202953457215
1101	3	0.5229188609402371
1101	4	-0.22917981094005313
1101	5	-0.700197066273482
1101	6	-0.2421964689669167
1101	7	-0.2326544292732251
1101	8	0.3041552431836152
1101	9	0.165235256087648
1101	10	-0.8624249462261346
1101	11	-0.07507240984439534
1101	12	-0.4525130952313674
1101	13	0.06731702065056311
1101	14	-0.2341882410572822
1101	15	0.03014658020580896
1101	16	0.0891604191236226
1101	17	0.07276013660307257
1101	18	-0.2181019674828093
1101	19	-0.17664072959775012
1101	20	0.683492831134167
1101	21	-0.619560422130462
1101	22	1.2677200672021378
1101	23	0.15920115279072178
1101	24	-0.15190051935780957
1101	25	0.044097053919028184
1101	26	0.8821179566898323
1101	27	0.24862669906300283
1101	28	0.4390158106596118
1101	29	0.2161386632158866
1101	30	1.442429379643034
1101	31	0.307532814286656
1101	32	0.9092516341043542
1101	33	0.46141546429173275
1101	34	0.019139047202436643
1101	35	1.6219833530253034
1101	36	2.098825352360244
1101	37	0.7678195522923303
1101	38	0.9168311833178961
1101	39	1.481342541031701
1101	40	0.7542733151803205
1101	41	1.4706473050807887
1101	42	-0.31911041175686705
1101	43	1.007409998743963
1101	44	1.3938729382774149
1101	45	0.3410005733940551
1101	46	-0.3769525868250412
1101	47	0.9468510018601428
1101	48	0.31683263666284134
1101	49	1.228487506374119
1101	50	0.5065249277227117
1101	51	1.29937709598169
1101	52	0.05718634339477191
1101	53	1.1919412678009849
1101	54	0.6835272803648706
1101	55	2.1781927353130826
1101	56	0.9091743668368579
1101	57	1.1480787842628395
1101	58	-0.0032735651970661195
1101	59	0.396621349753584
1101	60	1.2852651574396852
1101	61	-0.3198461735431195
1102	1	-0.3767754449927949
1102	2	-0.3726325159199675
1102	3	0.16180774982912993
1102	4	0.3086989769387358
1102	5	0.12051000443358362
1102	6	-0.04775202452246674
1102	7	0.15623445961566773
1102	8	0.3647613037896704
1102	9	-0.18830009744770138
1102	10	0.09969626589507641
1102	11	0.2860387012667118
1102	12	-0.42978582250409403
1102	13	-0.08167287833934012
1102	14	0.22793297106392885
1102	15	0.04024759030681935
1102	16	0.13208971205291675
1102	17	0.07781064165357776
1102	18	0.5773525779717303
1102	19	0.19204613908911483
1102	20	-2.508426360785027
1102	21	-3.0084493110193513
1102	22	-0.8913708418887722
1102	23	-0.5428190492294789
1102	24	-0.22765809511538038
1102	25	-2.9836807238587504
1102	26	-0.4714173968455242
1102	27	-0.04177734134103872
1102	28	-1.3791660075222048
1102	29	-1.2409320438548193
1102	30	-1.94140900419535
1102	31	-1.5535782968244547
1102	32	-1.6968089719562514
1102	33	0.797274050150321
1102	34	0.6403511684145542
1102	35	-1.5648853338433852
1102	36	-1.9011746476397562
1102	37	-1.4417764073036317
1102	38	0.06077057725728707
1102	39	-1.0893645296753718
1102	40	-0.026029715122710684
1102	41	-0.953595119161637
1102	42	-0.42264576529222
1102	43	0.6058948472288108
1102	44	-1.7273391829347062
1102	45	-0.5327368003433186
1102	46	-1.5385687484412038
1102	47	-0.835977280968141
1102	48	0.12491344474365107
1102	49	-1.137674109787497
1102	50	0.13278755398533804
1102	51	-1.1046633080587132
1102	52	-0.08927830306987516
1102	53	-1.21209913623942
1102	54	0.5522141490517392
1102	55	-0.22584766872732231
1102	56	-0.47718926952677876
1102	57	0.1758565620406145
1102	58	-0.12701093893443982
1102	59	0.2754092285414629
1102	60	-1.0430176708431436
1102	61	-1.0218663755633202
1103	1	0.5020124337950804
1103	2	-0.45838364422429123
1103	3	0.2610985299709725
1103	4	0.20876345147129172
1103	5	0.059259198501926846
1103	6	0.16501393292433875
1103	7	-0.2834818524410707
1103	8	-0.07882348022064178
1103	9	-0.21537940112275322
1103	10	-0.18334693204173647
1103	11	-0.14658541220846644
1103	12	0.2903947061870724
1103	13	-0.16613451599246787
1103	14	0.2285777163895233
1103	15	0.16017022086774801
1103	16	0.3899878422914753
1103	17	-0.05371740476807929
1103	18	0.2446639899639962
1103	19	-0.991061533380261
1103	20	1.0357386940183275
1103	21	1.3532528166402251
1103	22	1.195025031741146
1103	23	0.10837372962286906
1103	24	-0.5313331434712794
1103	25	1.9879504818150089
1103	26	0.4873189023163107
1103	27	0.15938320024503838
1103	28	0.7297959525035829
1103	29	-0.06104809801342981
1103	30	0.8916019564751849
1103	31	1.3613153201968196
1103	32	1.5475495064447813
1103	33	0.7811554170104102
1103	34	0.09360713230881856
1103	35	0.9399502560985873
1103	36	1.3966976927857786
1103	37	0.6478431929542694
1103	38	-0.07784966774593727
1103	39	-0.14158890104867794
1103	40	1.103564095322163
1103	41	0.6692288653644773
1103	42	0.9048848601107444
1103	43	-0.41280276721348486
1103	44	0.9328800304759959
1103	45	1.4840265781221866
1103	46	-0.12399750408272325
1103	47	0.4870401271556517
1103	48	-0.7919144082544172
1103	49	-0.06997585059987443
1103	50	-0.3539951668399368
1103	51	0.9494952992913817
1103	52	0.2427655396122681
1103	53	-0.18630932321556415
1103	54	1.149248320553994
1103	55	-0.4979301961289977
1103	56	0.11662117534749328
1103	57	0.11911897338813304
1103	58	-1.2804603264263825
1103	59	0.8647064561365632
1103	60	0.3502769777706547
1103	61	-0.35648919954784475
1104	1	0.05130820844296835
1104	2	-0.2544080174137804
1104	3	0.039742022129594545
1104	4	0.0701159637078348
1104	5	-0.4697588816099483
1104	6	-0.4233388789825625
1104	7	-0.36019746526696395
1104	8	-0.0996006253609849
1104	9	0.016565459530525573
1104	10	0.0471994669194018
1104	11	-0.22569838793516084
1104	12	-0.12974314217971994
1104	13	-0.49097718905210286
1104	14	-0.2470990391793535
1104	15	0.13499791040925402
1104	16	-0.45348433830986323
1104	17	-0.04852311848299706
1104	18	-0.25918177968938494
1104	19	-0.1899427640265472
1104	20	0.32512037417015804
1104	21	-0.8065713767470513
1104	22	0.0740580953711536
1104	23	-0.5411118362546645
1104	24	-0.6565953550385615
1104	25	-0.6033990337179063
1104	26	-0.3745330605245627
1104	27	-0.7869758048493622
1104	28	-0.3919701048333444
1104	29	-0.3992760472692467
1104	30	0.5425858741657272
1104	31	1.1177832055229615
1104	32	1.303617831287454
1104	33	0.7106329916782741
1104	34	0.030876136404309307
1104	35	0.2033605048249889
1104	36	-0.7415502344942162
1104	37	0.4160981125427199
1104	38	-0.45523454438163924
1104	39	-0.2502693525520243
1104	40	-0.08492856275395155
1104	41	0.6114923755033246
1104	42	-0.2975924148867577
1104	43	-0.5524491561856131
1104	44	0.43143162372342303
1104	45	-1.0901418366215943
1104	46	0.1848627496381816
1104	47	1.1213423946613936
1104	48	0.8974273158490718
1104	49	0.5942934531659816
1104	50	-0.24151889074364163
1104	51	-0.3031268163813756
1104	52	0.12369651553874661
1104	53	0.7678411113064634
1104	54	0.8509764196449972
1104	55	-0.7952031958293269
1104	56	0.39391615087441423
1104	57	-0.9884626867856738
1104	58	-0.24779625690285556
1104	59	-1.077557054002284
1104	60	0.23440443756487994
1104	61	-0.44738920953685835
1201	1	0.17097795103646263
1201	2	0.06477605252307939
1201	3	-0.21980144557316805
1201	4	0.4104753614737433
1201	5	-0.9095840394535628
1201	6	0.06719050421316553
1201	7	0.07807354007543665
1201	8	0.3639253581261386
1201	9	0.5001011564707909
1201	10	0.22320723768191186
1201	11	-0.12545555160684785
1201	12	-0.11400734810492708
1201	13	-0.6110354697708971
1201	14	0.24109911526455363
1201	15	0.36501248058895186
1201	16	-0.1278893893054942
1201	17	0.35283676495556904
1201	18	0.3675302164252372
1201	19	0.2160795769156607
1201	20	0.017592447992402782
1201	21	1.3892518384059365
1201	22	0.3349614465124837
1201	23	-0.7254731767111977
1201	24	0.3848810898375916
1201	25	-0.6319565859277176
1201	26	-0.1301425797086324
1201	27	-0.45807828177990473
1201	28	-0.13339798244383516
1201	29	-0.7790720647534641
1201	30	0.9313182685319248
1201	31	0.8211343468537038
1201	32	0.09545853065607801
1201	33	1.2221434336403902
1201	34	-0.20729773440675814
1201	35	-0.6213116661317848
1201	36	-1.6827838430420528
1201	37	-0.20478581169234644
1201	38	-0.23201939139475058
1201	39	-0.48455784211006403
1201	40	-0.7106692135553132
1201	41	-0.2741802811261067
1201	42	-0.6882674998794727
1201	43	0.4194789642612058
1201	44	0.48582696126591784
1201	45	0.17280133967758005
1201	46	-0.9279104412311732
1201	47	0.35106556124711474
1201	48	1.3015069661647587
1201	49	0.43557562897948543
1201	50	-1.0444329266834202
1201	51	-0.012500298654323672
1201	52	0.21772274186220741
1201	53	0.02029375822244006
1201	54	0.7256728742346006
1201	55	-0.11759270529994481
1201	56	-0.12128540327808679
1201	57	-0.32989056439616604
1201	58	-0.35250728167216394
1201	59	-1.143608535303887
1201	60	0.31016937199907346
1201	61	-1.5677388938496328
1202	1	0.17097795103646263
1202	2	0.2854657076954936
1202	3	0.08364683028889885
1202	4	0.21737191319787996
1202	5	0.4145538915809226
1202	6	0.06719050421316553
1202	7	-0.11502990820042669
1202	8	-0.07745395221868279
1202	9	-0.024036774563690244
1202	10	-0.19058586576636571
1202	11	-0.09786934471029696
1202	12	-0.2795245894842395
1202	13	0.4096541854015143
1202	14	-0.2554526088733766
1202	15	-0.24188407113518906
1202	16	-0.32099283758135755
1202	17	0.1597333166797057
1202	18	0.06408194056316319
1202	19	-0.28047214722226954
1202	20	1.783109689371713
1202	21	-0.2107481615940614
1202	22	0.6659959292711051
1202	23	0.07452682328880655
1202	24	0.30212246914793894
1202	25	1.1059744485550418
1202	26	0.1457194892568836
1202	27	-0.5684231093661154
1202	28	1.7424640865216823
1202	29	-1.5790720647534613
1202	30	2.614076889221579
1202	31	-0.39265875659457095
1202	32	1.2816654272078019
1202	33	-0.3502703594630532
1202	34	-0.45557359647572326
1202	35	1.4476538511095924
1202	36	0.27583684661311736
1202	37	1.8365934986524834
1202	38	-0.28719180518785237
1202	39	1.4464766406485587
1202	40	0.7238135450653758
1202	41	1.3534059257704456
1202	42	1.1324221552929394
1202	43	-1.8425900012560383
1202	44	1.478930409541782
1202	45	-0.9306469361844911
1202	46	0.5617447311826211
1202	47	0.571755216419529
1202	48	-0.795044757973173
1202	49	1.7321273531174164
1202	50	-0.07891568530410709
1202	51	0.20818935651809056
1202	52	-0.9408979477929655
1202	53	1.040983413394855
1202	54	0.47739701216563546
1202	55	0.7651659153897121
1202	56	0.9821628725839844
1202	57	-0.5781664264651312
1202	58	0.006113407983011854
1202	59	-0.42636715599354247
1202	60	0.7515486823438984
1202	61	-1.3194630317806642
1203	1	0.012216515427731167
1203	2	-0.01085143985318382
1203	3	-0.015062998470192213
1203	4	0.08544603940008244
1203	5	-0.4490632794254097
1203	6	-0.22462277282178889
1203	7	-0.10226894174488166
1203	8	0.2089171479455203
1203	9	0.013307818445923658
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2403	36	0.0054920190269101
2403	37	-1.27329155881878
2403	38	2.3334978499845604
2403	39	-0.5875463478571881
2403	40	-1.9557266848196804
2403	41	-0.6493526949192123
2403	42	-0.40688818953464434
2403	43	1.0374099987439642
2403	44	-1.3661270617225867
2403	45	-0.2612216488281689
2403	46	0.7208251909527377
2403	47	-0.668704553695413
2403	48	-1.038722918892713
2403	49	-0.1970680491814356
2403	50	-0.2490306278328447
2403	51	-0.9495117929071988
2403	52	-0.07836921216078352
2403	53	-0.6436142877545699
2403	54	-0.7520282751906855
2403	55	-0.150696153575808
2403	56	-0.6941589664964773
2403	57	-0.3041434379593859
2403	58	0.21117087924737632
2403	59	0.5032880164202531
2403	60	-0.4769570647825372
2403	61	1.2690427153457726
2404	1	-0.17445815444021662
2404	2	-0.1703152253673892
2404	3	-0.28917264232773476
2404	4	0.2855260714128889
2404	5	0.5857506461448168
2404	6	0.14832640685008158
2404	7	0.23466583216468706
2404	8	-0.029178090149720504
2404	9	-0.0733268354156209
2404	10	0.14247701455817463
2404	11	-0.2629809065764235
2404	12	0.10140847339608428
2404	13	3.235565982748767E-4
2404	14	0.35894901384467914
2404	15	0.026878606349598044
2404	16	-0.5301206266280118
2404	17	0.09563595359654187
2404	18	0.05150587565444198
2404	19	0.12074489131727972
2404	20	0.4514666873433164
2404	21	1.246452649764965
2404	22	-0.4234564033860977
2404	23	-0.5597530955752887
2404	24	0.24025634338729418
2404	25	0.4869075114353656
2404	26	0.4866931200885247
2404	27	-0.19418375845333458
2404	28	0.6448981636007893
2404	29	0.4399948723662135
2404	30	0.03720062147310088
2404	31	-0.779068492902887
2404	32	0.40925163410435417
2404	33	0.5610886669061159
2404	34	0.715217478574985
2404	35	0.7265585164239958
2404	36	1.3831390778504407
2404	37	0.5341594215733743
2404	38	-0.019443326486026535
2404	39	1.5434340442996728
2404	40	-0.6329815867804633
2404	41	-0.20582328315450482
2404	42	0.6237000457594704
2404	43	-0.2131782365501529
2404	44	1.344853330434276
2404	45	-0.4302412566713052
2404	46	0.528276171344892
2404	47	-0.17733200467580446
2404	48	-0.19950723261820258
2404	49	-0.00844059820104448
2404	50	0.7646948623632355
2404	51	0.371272520818291
2404	52	-0.04738882000392053
2404	53	0.008934731853273092
2404	54	-0.23477337322990266
2404	55	-0.29893144769345525
2404	56	0.1983900531113676
2404	57	-0.6574767712927176
2404	58	0.4460728400316931
2404	59	1.2397586046555453
2404	60	0.7493174450213829
2404	61	1.5886505584830246
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
14	1.0
15	9.0
16	39.0
17	69.0
18	95.0
19	122.0
20	157.0
21	242.0
22	436.0
23	641.0
24	1193.0
25	2985.0
26	8852.0
27	10929.0
28	6071.0
29	1912.0
30	1286.0
31	1441.0
32	1613.0
33	963.0
34	638.0
35	577.0
36	791.0
37	630.0
38	731.0
39	1808.0
40	1076.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	4.367978458074911	80.23484229809964	7.963449356611561	7.433729887213897
2	75.87569249784801	3.4564195378197633	10.267729048491404	10.40015891584082
3	4.840311651621162	75.42763811331582	8.049528770388683	11.682521464674332
4	4.9241839009424595	4.707881784271746	81.3913964729512	8.976537841834595
5	2.8030988588959764	4.902112256384223	8.32983865627828	83.96495022844152
6	77.13156907321165	4.491579667601033	9.70269494780056	8.674156311386762
7	76.67027170194451	5.248637075948529	6.398569757432626	11.682521464674332
8	5.058820932747699	75.83154920873154	11.854680292228574	7.254949566292185
9	4.743196415564924	78.47131789789657	6.257311232259916	10.528174454278588
10	75.7609199461452	9.691659125521443	6.44933453991657	8.0980863884168
11	5.74524907850884	80.23484229809964	6.032180457765908	7.987728165625621
12	77.31917805195665	7.3035071843203045	5.038956452645286	10.338358311077759
13	6.0719094179707325	9.111174873639834	6.8355883196857	77.98132738870373
14	77.74736795638643	9.755666894740326	5.833535656741784	6.663429492131459
15	6.188889134129385	7.744940075485024	76.57757079479993	9.488599995585671
16	5.8070496832719005	9.998454984880924	7.058511929723883	77.13598340212329
17	78.52428984483633	9.411349239631845	5.138278853157349	6.926082062374468
18	7.6698964839870225	7.793497693513143	76.48486988765532	8.051735934844505
19	77.70543183172578	9.998454984880924	7.493323327521134	4.802789855872161
20	20.116096850376323	10.16840664797934	63.709802017348316	6.005694484296025
21	10.73785507758183	75.0612488136491	5.848985807932549	8.351910300836515
22	79.47778488975213	11.468426512459445	4.381221444809853	4.672567152978568
23	82.73997395545942	8.52627629284658	4.167126492594964	4.566623259099035
24	80.65420354470612	10.539210276557705	4.6438740150528615	4.162712163683316
25	18.818284150352042	73.15646588827333	4.014832145143134	4.010417816231487
26	12.08422539563423	80.31871454742094	3.8846094422495416	3.7124506146953005
27	11.146180501909198	80.38051515218399	4.153883505860022	4.319420840046792
28	21.38742357693072	69.94504160505	3.8250160019423043	4.842518816076986
29	15.520780453351579	76.49590570993445	4.579866245833977	3.403447590879997
30	44.880482044717155	43.96009446663871	6.588385900633456	4.571037588010682
31	15.982077824618713	6.1933034630410315	72.59584611649414	5.228772595846117
32	75.10097777385393	6.016730306575143	15.69293928090582	3.189352638665107
33	11.051272430308783	9.515085969055555	75.38349482419935	4.050146776436312
34	11.501533979296799	9.623237027390912	75.92645728033196	2.948771712980334
35	27.620456000176574	9.13324651819807	59.14759308716092	4.0987043944644315
36	12.647052331869247	10.910013905136072	7.541880945549252	68.90105281744543
37	15.57595956474717	30.73035071843203	3.7411437526210083	49.95254596419979
38	10.777584037786655	12.236519743086058	4.85134747390028	72.13454874522701
39	14.145716997373475	8.844107974485178	22.13123799854327	54.87893702959808
40	11.130730350718432	10.96960734544331	72.09261262056636	5.8070496832719005
41	25.483920806939324	9.170768313947072	62.720992341139336	2.6243185379742644
42	76.38334032268745	12.046703599885229	9.61661553402344	1.9533405434038893
43	14.27152537135542	9.159732491667954	73.92455911889995	2.644183018076677
44	46.36811088794226	11.470633676915266	39.46410047012603	2.6971549650164435
45	11.19032379102567	10.00286931379257	74.4344141081952	4.372392786986558
46	13.55419692321275	13.633654843622397	8.607941377712052	64.2042068554528
47	49.06305868850288	38.67393559494118	8.614562871079524	3.648442845476416
48	8.727128258326527	73.3396605381067	13.10172820976891	4.831482993797867
49	7.8332266537179684	6.789237866113404	36.8331604387843	48.544375041384335
50	9.515085969055555	4.650495508420333	12.371156774891297	73.46326174763281
51	9.508464475688083	5.010263314719579	39.33608493168826	46.14518727790408
52	8.378396274306398	7.76701172004326	9.636480014125851	74.2181119915245
53	24.883572074955303	12.002560310768756	13.057584920652438	50.0562826936235
54	69.9913920586223	11.678107135762685	12.35349945924471	5.977001346370319
55	8.978745006290419	8.142229677533273	76.8755379963361	6.003487319840201
56	44.41918467345002	5.314852009623237	44.83413159114485	5.431831725781889
57	73.86055134968107	5.473767850442536	12.878804599730726	7.786876200145673
58	9.766702717019445	4.948462709956519	78.2594301101375	7.025404462886529
59	7.277021210850421	4.5423444500849754	18.604189198137153	69.57644514092745
60	31.67060277661288	9.618822698479264	46.061315028582776	12.649259496325072
61	10.51713863199947	4.637252521685391	9.097931886904894	75.74767695941024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.5
17	2.0
18	1.0
19	1.0
20	3.5
21	6.0
22	6.0
23	6.0
24	7.5
25	9.0
26	9.0
27	6.5
28	4.0
29	6.5
30	7.0
31	5.0
32	6.0
33	7.0
34	161.5
35	198.5
36	81.0
37	46.0
38	11.0
39	14.0
40	58.5
41	100.0
42	188.5
43	611.5
44	946.0
45	1577.5
46	2209.0
47	3508.0
48	6654.5
49	8502.0
50	8657.5
51	8813.0
52	8594.0
53	6970.0
54	5565.0
55	4261.5
56	2958.0
57	2123.0
58	912.0
59	536.0
60	354.0
61	123.0
62	74.0
63	58.0
64	42.0
65	38.5
66	33.5
67	32.0
68	26.0
69	20.0
70	12.0
71	7.0
72	10.0
73	7.0
74	4.0
75	3.5
76	4.5
77	6.0
78	3.0
79	0.0
80	0.0
81	1.0
82	2.0
83	1.5
84	0.5
85	0.0
86	0.5
87	1.0
88	1.5
89	1.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	2.5
99	17.5
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	45307.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.42507780254707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.16592427616926	55.28505528946962
2	6.3369988864142535	8.038492948109564
3	2.404649220489978	4.575451916922329
4	1.0753062360801782	2.728055267397974
5	0.6611915367483295	2.096806233032423
6	0.4315144766146993	1.642130355132761
7	0.28535634743875277	1.2669123976427483
8	0.21923719376391984	1.112410885735096
9	0.22271714922048996	1.2713267265543955
>10	1.092706013363029	12.852318626260843
>50	0.07655902004454343	3.2577747367956387
>100	0.024359688195991092	3.4166905776149377
>500	0.0	0.0
>1k	0.0034799554565701557	2.4565740393316706
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	1113	2.4565740393316706	No Hit
AGATCGGAAGAGCGTCGTTGTAGGGAAAGAGTGTTGCACTTGGTGTAGATCTCGGTGGTCG	400	0.8828657823294414	No Hit
AGTGAGGTAAAATAATCGAGAAGTGAGAATGGATTCGAACTAATGGAATTGGAGAAAATAG	306	0.6753923234820227	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTTGTCTC	291	0.6422848566446686	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTTGTCGC	164	0.36197497075507096	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGACCCCCGTTGTCTC	159	0.350939148475953	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCAACCCCCGTTGTCTC	126	0.278102721433774	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTTGTCTC	102	0.22513077449400754	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTTGTCGC	95	0.20968062330324233	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCGACCCCCGTTGTCTC	88	0.1942304721124771	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTGGTCTC	84	0.18540181428918268	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATTTTTCCCCTTGTTTCAACCCCCGTTGTCTC	77	0.16995166309841747	Illumina Single End PCR Primer 1 (96% over 29bp)
CGGATATTTTGGCAGCATTCCGAGTAACTCCTCAACCAAGATCGGAAGAGCGTCGTGTAGG	76	0.16774449864259386	Illumina Single End PCR Primer 1 (100% over 23bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCTCCGTTGTCTC	74	0.16333016973094663	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCGACCCCCGTTGTCTC	70	0.15450151190765224	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGACCTCCGTTGTCTC	69	0.15229434745182863	Illumina Single End PCR Primer 1 (96% over 29bp)
GCCAAGATATCTGTATCCTTGGTCTGATATTCAGGAGTATAATAAGTTAATCAGATCGGAA	69	0.15229434745182863	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTGGTCGC	67	0.14788001854018143	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGACCCCCGTTGTCGC	66	0.14567285408435782	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCGATCTCCGTTGTCTC	64	0.14125852517271062	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCATTGTCTC	62	0.13684419626106342	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTGTCAACCCCCGTTGTCTC	61	0.1346370318052398	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCAACCCCCGTTGTCGC	60	0.13242986734941622	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCGACCCCCGTGGTCTC	60	0.13242986734941622	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTGGTCTC	59	0.1302227028935926	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTGGTCGC	57	0.1258083739819454	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCGACCTCCGTTGTCTC	57	0.1258083739819454	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAATCCCCGTTGTCTC	56	0.12360120952612179	Illumina Single End PCR Primer 1 (96% over 29bp)
TGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGAGATCGGAAGAG	53	0.11697971615865098	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCGACCCCCGTGGTCGC	52	0.11477255170282738	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCAACCTCCGTTGTCTC	48	0.10594389387953297	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATTTTTCCCCTTGTTTCGACCCCCGTTGTCTC	47	0.10373672942370936	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGATCCCCGTTGTCTC	47	0.10373672942370936	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTTAGGGAAAGAGTGTTGCACTTGGTGTAGATCTCGGTGGTCGCC	46	0.10152956496788576	Illumina Single End PCR Primer 1 (95% over 22bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGATCTCCGTTGTCTC	46	0.10152956496788576	Illumina Single End PCR Primer 1 (96% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	70.37985300284724	0.0	0.0	0.0	0.0
2	70.68002736883925	0.0	0.0	0.0	0.0
3	70.7970070849979	0.0	0.0	0.0	0.0
4	70.86984351204008	0.0	0.0	0.0	0.0
5	70.99785905047786	0.0	0.0	0.0	0.0
6	71.27816893636745	0.0	0.0	0.0	0.0
7	71.65117972940163	0.0	0.0	0.0	0.0
8	72.40823713774914	0.0	0.0	0.0	0.0
9	72.88719182466285	0.0	0.0	0.0	0.0
10	73.44781159644205	0.0	0.0	0.0	0.0
11	73.95325225682565	0.0	0.0	0.0	0.0
12	74.71030966517316	0.0	0.0	0.0	0.0
13	75.5843467896793	0.0	0.0	0.0	0.0
14	76.67247886640034	0.0	0.0	0.0	0.0
15	78.08947844703908	0.0	0.0	0.0	0.0
16	79.8861103140795	0.0	0.0	0.0	0.0
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50	95.85935948087491	0.01324298673494162	0.0	0.0	0.0
>>END_MODULE