FASTQC-Report Basic StatisticsFilenameAM-AL-LC2_S109_R2_001.fastq.gzFile typeConventional base callsEncodingSanger / Illumina 1.9Total Sequences45307Sequences flagged as poor quality0Sequence length61%GC51Per base sequence qualityPer tile sequence qualityPer sequence quality scoresPer base sequence contentPer sequence GC contentPer base N contentSequence Length DistributionSequence Duplication LevelsOverrepresented sequencesATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG11132.4565740393316706No HitAGATCGGAAGAGCGTCGTTGTAGGGAAAGAGTGTTGCACTTGGTGTAGATCTCGGTGGTCG4000.8828657823294414No HitAGTGAGGTAAAATAATCGAGAAGTGAGAATGGATTCGAACTAATGGAATTGGAGAAAATAG3060.6753923234820227No HitAGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTTGTCTC2910.6422848566446686Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTTGTCGC1640.36197497075507096Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGACCCCCGTTGTCTC1590.350939148475953Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCAACCCCCGTTGTCTC1260.278102721433774Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTTGTCTC1020.22513077449400754Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTTGTCGC950.20968062330324233Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCGACCCCCGTTGTCTC880.1942304721124771Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTGGTCTC840.18540181428918268Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATTTTTCCCCTTGTTTCAACCCCCGTTGTCTC770.16995166309841747Illumina Single End PCR Primer 1 (96% over 29bp)CGGATATTTTGGCAGCATTCCGAGTAACTCCTCAACCAAGATCGGAAGAGCGTCGTGTAGG760.16774449864259386Illumina Single End PCR Primer 1 (100% over 23bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCTCCGTTGTCTC740.16333016973094663Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCGACCCCCGTTGTCTC700.15450151190765224Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGACCTCCGTTGTCTC690.15229434745182863Illumina Single End PCR Primer 1 (96% over 29bp)GCCAAGATATCTGTATCCTTGGTCTGATATTCAGGAGTATAATAAGTTAATCAGATCGGAA690.15229434745182863No HitAGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTGGTCGC670.14788001854018143Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGACCCCCGTTGTCGC660.14567285408435782Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCGATCTCCGTTGTCTC640.14125852517271062Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCATTGTCTC620.13684419626106342Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTGTCAACCCCCGTTGTCTC610.1346370318052398Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCAACCCCCGTTGTCGC600.13242986734941622Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCGACCCCCGTGGTCTC600.13242986734941622Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTGGTCTC590.1302227028935926Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTGGTCGC570.1258083739819454Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCGACCTCCGTTGTCTC570.1258083739819454Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAATCCCCGTTGTCTC560.12360120952612179Illumina Single End PCR Primer 1 (96% over 29bp)TGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGAGATCGGAAGAG530.11697971615865098No HitAGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCGACCCCCGTGGTCGC520.11477255170282738Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCAACCTCCGTTGTCTC480.10594389387953297Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATTTTTCCCCTTGTTTCGACCCCCGTTGTCTC470.10373672942370936Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGATCCCCGTTGTCTC470.10373672942370936Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTTAGGGAAAGAGTGTTGCACTTGGTGTAGATCTCGGTGGTCGCC460.10152956496788576Illumina Single End PCR Primer 1 (95% over 22bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGATCTCCGTTGTCTC460.10152956496788576Illumina Single End PCR Primer 1 (96% over 29bp)Adapter Content