FastQCFastQC Report
Mon 8 Jul 2024
AM-AL-LC2_S109_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-LC2_S109_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45307
Sequences flagged as poor quality0
Sequence length61
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG11132.4565740393316706No Hit
AGATCGGAAGAGCGTCGTTGTAGGGAAAGAGTGTTGCACTTGGTGTAGATCTCGGTGGTCG4000.8828657823294414No Hit
AGTGAGGTAAAATAATCGAGAAGTGAGAATGGATTCGAACTAATGGAATTGGAGAAAATAG3060.6753923234820227No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTTGTCTC2910.6422848566446686Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTTGTCGC1640.36197497075507096Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGACCCCCGTTGTCTC1590.350939148475953Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCAACCCCCGTTGTCTC1260.278102721433774Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTTGTCTC1020.22513077449400754Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTTGTCGC950.20968062330324233Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCGACCCCCGTTGTCTC880.1942304721124771Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTGGTCTC840.18540181428918268Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATTTTTCCCCTTGTTTCAACCCCCGTTGTCTC770.16995166309841747Illumina Single End PCR Primer 1 (96% over 29bp)
CGGATATTTTGGCAGCATTCCGAGTAACTCCTCAACCAAGATCGGAAGAGCGTCGTGTAGG760.16774449864259386Illumina Single End PCR Primer 1 (100% over 23bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCTCCGTTGTCTC740.16333016973094663Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCGACCCCCGTTGTCTC700.15450151190765224Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGACCTCCGTTGTCTC690.15229434745182863Illumina Single End PCR Primer 1 (96% over 29bp)
GCCAAGATATCTGTATCCTTGGTCTGATATTCAGGAGTATAATAAGTTAATCAGATCGGAA690.15229434745182863No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTGGTCGC670.14788001854018143Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGACCCCCGTTGTCGC660.14567285408435782Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCGATCTCCGTTGTCTC640.14125852517271062Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCATTGTCTC620.13684419626106342Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTGTCAACCCCCGTTGTCTC610.1346370318052398Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCAACCCCCGTTGTCGC600.13242986734941622Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCGACCCCCGTGGTCTC600.13242986734941622Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCAACCCCCGTGGTCTC590.1302227028935926Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAACCCCCGTGGTCGC570.1258083739819454Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCGACCTCCGTTGTCTC570.1258083739819454Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCAATCCCCGTTGTCTC560.12360120952612179Illumina Single End PCR Primer 1 (96% over 29bp)
TGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGAGATCGGAAGAG530.11697971615865098No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTGTCGACCCCCGTGGTCGC520.11477255170282738Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCCCCTTGTTTCAACCTCCGTTGTCTC480.10594389387953297Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATTTTTCCCCTTGTTTCGACCCCCGTTGTCTC470.10373672942370936Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGATCCCCGTTGTCTC470.10373672942370936Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTTAGGGAAAGAGTGTTGCACTTGGTGTAGATCTCGGTGGTCGCC460.10152956496788576Illumina Single End PCR Primer 1 (95% over 22bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCCCCTTGTTTCGATCTCCGTTGTCTC460.10152956496788576Illumina Single End PCR Primer 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)