##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-LC2_S109_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45307
Sequences flagged as poor quality	0
Sequence length	61
%GC	59
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.07109276712208	40.0	40.0	40.0	40.0	40.0
2	38.96250027589556	40.0	40.0	40.0	40.0	40.0
3	38.66629880592403	40.0	40.0	40.0	40.0	40.0
4	39.08808793343192	40.0	40.0	40.0	40.0	40.0
5	39.07961242192156	40.0	40.0	40.0	40.0	40.0
6	38.841680093583776	40.0	40.0	40.0	40.0	40.0
7	39.000066214933675	40.0	40.0	40.0	40.0	40.0
8	38.763458185269386	40.0	40.0	40.0	40.0	40.0
9	39.079788995078026	40.0	40.0	40.0	40.0	40.0
10	39.201271326726555	40.0	40.0	40.0	40.0	40.0
11	39.23720396406736	40.0	40.0	40.0	40.0	40.0
12	39.28072924713621	40.0	40.0	40.0	40.0	40.0
13	38.94709426799391	40.0	40.0	40.0	40.0	40.0
14	39.21963493499901	40.0	40.0	40.0	40.0	40.0
15	39.37616703820602	40.0	40.0	40.0	40.0	40.0
16	39.32504910940914	40.0	40.0	40.0	40.0	40.0
17	39.34526673582449	40.0	40.0	40.0	40.0	40.0
18	39.33281832829364	40.0	40.0	40.0	40.0	40.0
19	39.2815238263403	40.0	40.0	40.0	40.0	40.0
20	38.93535215308893	40.0	40.0	40.0	40.0	40.0
21	39.50577173505198	40.0	40.0	40.0	40.0	40.0
22	39.11219016928951	40.0	40.0	40.0	40.0	40.0
23	39.06292625863553	40.0	40.0	40.0	40.0	40.0
24	39.24409031716953	40.0	40.0	40.0	40.0	40.0
25	39.56757233981504	40.0	40.0	40.0	40.0	40.0
26	39.48873242545302	40.0	40.0	40.0	40.0	40.0
27	39.54850243891672	40.0	40.0	40.0	40.0	40.0
28	39.50744918003841	40.0	40.0	40.0	40.0	40.0
29	39.30951067164015	40.0	40.0	40.0	40.0	40.0
30	39.29344251440175	40.0	40.0	40.0	40.0	40.0
31	39.42551923543823	40.0	40.0	40.0	40.0	40.0
32	39.5473547133997	40.0	40.0	40.0	40.0	40.0
33	39.15350828790253	40.0	40.0	40.0	40.0	40.0
34	39.35780342993356	40.0	40.0	40.0	40.0	40.0
35	39.5002096806233	40.0	40.0	40.0	40.0	40.0
36	39.220164654468405	40.0	40.0	40.0	40.0	40.0
37	39.338468669300546	40.0	40.0	40.0	40.0	40.0
38	38.867194914693094	40.0	40.0	40.0	40.0	40.0
39	38.124439932019335	40.0	40.0	40.0	24.0	40.0
40	39.282318405544395	40.0	40.0	40.0	40.0	40.0
41	38.372436930275676	40.0	40.0	40.0	40.0	40.0
42	39.051537290043484	40.0	40.0	40.0	40.0	40.0
43	39.096563444942284	40.0	40.0	40.0	40.0	40.0
44	38.85624737899221	40.0	40.0	40.0	40.0	40.0
45	27.656300350939148	24.0	24.0	40.0	12.0	40.0
46	32.2044717151875	40.0	24.0	40.0	24.0	40.0
47	37.59233672500938	40.0	40.0	40.0	24.0	40.0
48	38.39080053854813	40.0	40.0	40.0	40.0	40.0
49	38.58211755357892	40.0	40.0	40.0	40.0	40.0
50	38.4571920453793	40.0	40.0	40.0	40.0	40.0
51	38.26119584170217	40.0	40.0	40.0	40.0	40.0
52	38.05690069967113	40.0	40.0	40.0	40.0	40.0
53	37.52506235239588	40.0	40.0	40.0	24.0	40.0
54	36.70037742512194	40.0	40.0	40.0	24.0	40.0
55	36.21515439115368	40.0	40.0	40.0	24.0	40.0
56	35.541969232127485	40.0	40.0	40.0	24.0	40.0
57	34.48835720749553	40.0	24.0	40.0	24.0	40.0
58	33.149049815701765	40.0	24.0	40.0	24.0	40.0
59	30.996181605491426	40.0	24.0	40.0	12.0	40.0
60	29.45028362063257	24.0	24.0	40.0	12.0	40.0
61	28.208621184364446	24.0	24.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.11609400455890295
1101	2	-0.13084732955730516
1101	3	0.6477194210472064
1101	4	-0.06010822115389658
1101	5	0.02335295344229138
1101	6	-0.6340389607255545
1101	7	-0.5399868846325973
1101	8	-0.13099048389185697
1101	9	-0.29274381517176806
1101	10	-0.3545906222803836
1101	11	0.47405220615911503
1101	12	-0.15876209212945014
1101	13	-0.6718145399448403
1101	14	0.5245148488886429
1101	15	-0.26876033940500577
1101	16	0.03122383556218722
1101	17	-0.12070895088022837
1101	18	-0.25043701097821014
1101	19	-0.028502677970394075
1101	20	-0.41856865327655157
1101	21	0.014156783721695376
1101	22	0.049232341674937175
1101	23	-0.6788177206510397
1101	24	0.07338905481648084
1101	25	-0.3390549811117083
1101	26	-0.2094265428553399
1101	27	-0.03643563010066231
1101	28	-0.10404883773980345
1101	29	-0.5887682298802588
1101	30	-0.004682485456775964
1101	31	0.20401006030858326
1101	32	-0.007267831267185443
1101	33	0.053692428460983876
1101	34	-0.2841114692434985
1101	35	-0.4837768673567453
1101	36	-0.17950109489528643
1101	37	-0.275395462518496
1101	38	-0.15149575605692434
1101	39	-0.27356310782239746
1101	40	-0.17216935427724422
1101	41	-0.04236864276296615
1101	42	-0.07090821295032157
1101	43	-0.4440895287981732
1101	44	-0.9135766948371398
1101	45	0.26041689449529315
1101	46	0.7304370583952888
1101	47	0.32657188392891356
1101	48	-0.06672532595896996
1101	49	-0.41656734682707963
1101	50	-0.19494622845651577
1101	51	-0.18503174455731397
1101	52	-0.47984380127378046
1101	53	0.021237635485150008
1101	54	-1.1003390049647805
1101	55	-0.4561700020690509
1101	56	-0.191769159465494
1101	57	-1.6156068213208599
1101	58	-1.3962897535325176
1101	59	-0.6590882426437652
1101	60	-0.13527812313386534
1101	61	1.6865058685262682
1102	1	0.0756899641548614
1102	2	0.19238499367502016
1102	3	-0.10985633652855142
1102	4	-0.3934415544872323
1102	5	-0.6306874505981099
1102	6	0.6033347766481825
1102	7	0.20748786284215015
1102	8	0.020524667623298853
1102	9	0.27038749795953976
1102	10	-0.08943910712886805
1102	11	-0.07140233929542461
1102	12	0.3664904331230687
1102	13	0.020104651974349963
1102	14	0.04976737414116883
1102	15	0.07972450907984552
1102	16	0.1221329264712736
1102	17	0.414644584473308
1102	18	0.03491652437532622
1102	19	0.05483065536294163
1102	20	-0.4640231987310983
1102	21	0.12526789483280254
1102	22	0.2765050689476638
1102	23	0.08633379450047585
1102	24	-0.2346917532643289
1102	25	0.04730865525192485
1102	26	-0.42659826002705614
1102	27	0.04437245070741369
1102	28	0.14342590973494396
1102	29	-0.18472782583985747
1102	30	0.2705700397957429
1102	31	-0.3389192326207109
1102	32	-0.1360557100550679
1102	33	0.3794500042185618
1102	34	0.41790873277670215
1102	35	-0.1453930289729115
1102	36	-0.40929907469326565
1102	37	-0.09105202817506353
1102	38	0.18941333485216916
1102	39	0.19360860934931878
1102	40	0.3278306457227558
1102	41	0.13187378147945594
1102	42	0.41899077694866804
1102	43	-0.17388750859615243
1102	44	0.2960192647588187
1102	45	-0.8279669438885477
1102	46	-1.0094619315037043
1102	47	0.05636986372689279
1102	48	0.3070120477784073
1102	49	0.12131144105170932
1102	50	0.4338416503313596
1102	51	0.6457763362507691
1102	52	0.2120753906454098
1102	53	0.04649016073766887
1102	54	-0.49427839890417147
1102	55	-0.7415235374225873
1102	56	-0.14631461401094725
1102	57	-0.5095462152602579
1102	58	0.621387014144247
1102	59	-1.5176741012296269
1102	60	-1.6327528706086127
1102	61	-0.8917769597565588
1103	1	0.09954554120192682
1103	2	-0.012644019864630707
1103	3	-0.018947245619465036
1103	4	0.25904071501631876
1103	5	-0.3070252971487193
1103	6	0.03853787142574561
1103	7	0.13850011300333165
1103	8	0.634966962916657
1103	9	0.39224436449725886
1103	10	0.3883171791380562
1103	11	0.3534848302725919
1103	12	0.2762260875395768
1103	13	0.1426062638376635
1103	14	0.2928363618910055
1103	15	0.22414746201343405
1103	16	0.2439897930089927
1103	17	-0.4209453574996118
1103	18	0.3837237455229783
1103	19	0.09738384685230272
1103	20	-0.16679560363115797
1103	21	0.11817569625124236
1103	22	0.08823943387351818
1103	23	0.28040213750499277
1103	24	0.3700793621450842
1103	25	0.18399466427836586
1103	26	0.3993205020619186
1103	27	0.06758328242888467
1103	28	-0.11705120180599238
1103	29	0.20792207744834457
1103	30	0.2086744885384917
1103	31	-0.22802303661812573
1103	32	-0.2714522284303058
1103	33	-0.2459529616099374
1103	34	-0.24746844323877326
1103	35	0.1486108394990424
1103	36	-0.16945381357140832
1103	37	0.15781966750514442
1103	38	0.21907161982959877
1103	39	-0.15004064919355642
1103	40	0.008681709552547545
1103	41	-0.363290628578568
1103	42	0.19268516766433663
1103	43	0.1510641355044271
1103	44	0.34824363613212483
1103	45	0.1475327337387924
1103	46	-0.27251802434703265
1103	47	0.22787212033553317
1103	48	-0.15655984132539658
1103	49	0.028468114165825398
1103	50	0.25008923253638926
1103	51	-0.39484261926180153
1103	52	0.4906053713030474
1103	53	-0.3700153195975986
1103	54	-0.96558723191513
1103	55	0.33520115632337877
1103	56	0.5635499894706797
1103	57	0.33120168931743876
1103	58	0.4730955892570776
1103	59	-0.1508140109652807
1103	60	-1.4644743406279552
1103	61	0.28165953282886846
1104	1	0.3304915006465379
1104	2	0.19153138718760943
1104	3	-0.09406461651148135
1104	4	0.3201734689869511
1104	5	-0.38314156924941045
1104	6	-0.3202674427286851
1104	7	-0.025119904976889984
1104	8	-0.628643066051481
1104	9	0.17008873568894955
1104	10	-0.05998968331324761
1104	11	-0.2912060098033251
1104	12	-0.22761968211380434
1104	13	0.4870274005872375
1104	14	0.32420185984325656
1104	15	-0.16782137226885396
1104	16	0.021834164200683404
1104	17	0.20636460154543812
1104	18	-0.12172026606427977
1104	19	-0.2925871850126498
1104	20	0.18002289601922428
1104	21	0.2332491154900822
1104	22	-0.21602587428750297
1104	23	-0.36309002112052013
1104	24	-0.556501399017634
1104	25	0.18559290621223568
1104	26	0.40011962302884996
1104	27	0.06998064532969295
1104	28	0.08452706210370309
1104	29	0.4081018796659066
1104	30	-0.32354007544113017
1104	31	0.0025233623430125363
1104	32	-0.12581243220615335
1104	33	-0.2667301067502805
1104	34	-0.12839941916525532
1104	35	-0.15905073277145476
1104	36	0.23090579086371577
1104	37	0.048939435760061656
1104	38	-0.5916366011273482
1104	39	-1.6284144380258425
1104	40	0.09778369736594783
1104	41	-0.574058783608038
1104	42	0.1411418652969374
1104	43	-0.15340095289832334
1104	44	-0.23791159311569743
1104	45	0.2044701026330067
1104	46	0.17918510221375072
1104	47	0.298794106151135
1104	48	-0.595676812656933
1104	49	-0.18769410739046322
1104	50	-0.41677721437201143
1104	51	0.04227654965239225
1104	52	0.3879183270517217
1104	53	0.35378849620501995
1104	54	-0.1472873617722925
1104	55	-0.42995716951819674
1104	56	-0.25514944115563765
1104	57	-0.7869683236682832
1104	58	0.23555688183524381
1104	59	-1.0737987277767864
1104	60	-0.9024925206299521
1104	61	-0.20120931144243315
1201	1	0.14904419612978614
1201	2	-0.06801208051515317
1201	3	0.34886884633456106
1201	4	-0.05321166942975708
1201	5	0.24998130593271384
1201	6	-0.18078225574470963
1201	7	0.2699748011911538
1201	8	0.26441181495872
1201	9	0.18407610819987497
1201	10	0.2873634007081236
1201	11	-0.4891661846454838
1201	12	-0.042286996344010674
1201	13	-0.5495923177226203
1201	14	-0.16360775647534354
1201	15	0.012848855997297903
1201	16	0.14616636429781948
1201	17	0.21568951655272173
1201	18	0.0589499622018721
1201	19	0.08701456340891411
1201	20	0.08832789844758793
1201	21	0.017221917821309773
1201	22	-0.08295156637103673
1201	23	0.1596880264754006
1201	24	-0.44576803330612336
1201	25	0.1074967430262248
1201	26	0.01624395522895128
1201	27	-0.4471635994493255
1201	28	-0.127420485249381
1201	29	0.1093160613074815
1201	30	-0.11062118277478561
1201	31	0.09577251241586282
1201	32	-0.14232530253155318
1201	33	0.4726579457023661
1201	34	-0.08449461100595101
1201	35	-0.035884147050232684
1201	36	0.24023070587099937
1201	37	0.3933784838416585
1201	38	-0.6945992043327891
1201	39	-0.7273945254469183
1201	40	0.10943984112505944
1201	41	-0.4992651944871014
1201	42	-0.06439480298863742
1201	43	0.08943537541638591
1201	44	0.5624769450096068
1201	45	-0.9259815729376584
1201	46	-0.21343267340548167
1201	47	-1.701205893848865
1201	48	-0.5801352876447936
1201	49	0.2851567911039581
1201	50	0.17574342671589704
1201	51	0.46228626310552556
1201	52	0.0055968117530369454
1201	53	-1.5898734756259643
1201	54	-0.16010911990730392
1201	55	-0.7586604235249936
1201	56	0.8047825646724363
1201	57	1.082094328104425
1201	58	0.2874267215632713
1201	59	1.3370803397317097
1201	60	0.18521996115732264
1201	61	1.7280767497523222
1202	1	-0.09923166593917898
1202	2	-0.09559828741171117
1202	3	-0.20285529159647098
1202	4	0.3054090202254116
1202	5	0.22239509903616295
1202	6	-0.2911270833309203
1202	7	0.04928514601873957
1202	8	-0.17696749538610845
1202	9	-0.39523423662770796
1202	10	-0.20918832342980664
1202	11	0.2556614015614187
1202	12	-0.014700789447459783
1202	13	-0.6047647315157221
1202	14	-0.5222284461305122
1202	15	-0.2905994198647761
1202	16	0.0634077436081597
1202	17	-0.3084484144817594
1202	18	-0.13415348607399125
1202	19	-0.29919233314280547
1202	20	-0.022016929138622743
1202	21	-0.3965711856269678
1202	22	-0.30364122154345097
1202	23	-0.11617404249011543
1202	24	0.18871472531456135
1202	25	-0.33388256731860366
1202	26	0.07141636902205306
1202	27	-0.6126808408286308
1202	28	-0.4308687611114479
1202	29	-0.11137359386493273
1202	30	-0.11062118277478561
1202	31	-0.12491714275655141
1202	32	-0.11473909563500229
1202	33	-0.21699722671142752
1202	34	-0.3879428868680179
1202	35	0.07446068053597088
1202	36	-0.09080377688761843
1202	37	-0.2686904816755842
1202	38	-0.0325302388155464
1202	39	-0.5894634909641638
1202	40	0.3301294962974737
1202	41	-0.1406445048319327
1202	42	0.04595002459756614
1202	43	0.31012503058880014
1202	44	-0.32028167568005017
1202	45	0.06712187533820568
1202	46	0.5038087059048664
1202	47	-1.0667231352281732
1202	48	-0.08358356350686336
1202	49	-0.3493259675167337
1202	50	-0.5690841594909983
1202	51	-0.6687482196530965
1202	52	0.1711140531323494
1202	53	-0.5967700273501038
1202	54	0.419201224920279
1202	55	-0.8965914580077481
1202	56	-1.2090105387758356
1202	57	-2.062733258102469
1202	58	0.2598405146667133
1202	59	0.5646665466282634
1202	60	-0.22857314229095493
1202	61	1.479800887683357
1203	1	-0.11293093875175231
1203	2	-0.29864778307204176
1203	3	0.17152894485672476
1203	4	-0.20296536401104248
1203	5	0.34024637747857867
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2403	36	-0.06505665045084186
2403	37	0.08349342637038859
2403	38	0.08517091060974025
2403	39	-0.18578533004462372
2403	40	-0.13883602094390568
2403	41	0.21429802390370156
2403	42	-0.13979710183921412
2403	43	0.49035491564627165
2403	44	-0.05913225039269321
2403	45	1.592639116717514
2403	46	-1.064007386049159
2403	47	0.43212743948446786
2403	48	-0.11116977040341425
2403	49	-0.5599006801604105
2403	50	-0.6049462284565124
2403	51	0.42274603322047
2403	52	0.415711754281773
2403	53	-0.2832068089592994
2403	54	-0.3136723382981117
2403	55	0.08271888681983341
2403	56	-0.17176915946549087
2403	57	0.8043931786791347
2403	58	0.3425991353563731
2403	59	0.09202286846734609
2403	60	-0.6763892342449758
2403	61	-1.6190496870292854
2404	1	-0.2531870411927315
2404	2	-0.13738327726971988
2404	3	-0.24443744169789738
2404	4	-0.29540233880095457
2404	5	-0.26259475897600737
2404	6	-0.3824049737974491
2404	7	-0.2426012637175674
2404	8	-0.7094218564408763
2404	9	-0.3891490439299403
2404	10	-0.41831611247646094
2404	11	0.1603267159630377
2404	12	-0.03457908559350642
2404	13	0.1386429763950261
2404	14	-0.45914528183030967
2404	15	0.44692593510480094
2404	16	0.34494932575826454
2404	17	0.010010003368137177
2404	18	0.16949762954467218
2404	19	0.25090908673548995
2404	20	0.1696666408410934
2404	21	0.01742475757790629
2404	22	-0.3527284426387851
2404	23	0.316280318564651
2404	24	-0.6161534288436528
2404	25	0.05800384241770473
2404	26	-0.22249843828017646
2404	27	0.0844794025790776
2404	28	0.18353286160660787
2404	29	-0.0430165958933344
2404	30	0.10479463872622574
2404	31	0.06838914527590845
2404	32	0.29175177657594986
2404	33	0.038986546108041864
2404	34	0.013274151671531342
2404	35	-0.09815595232407048
2404	36	-0.40662527790181713
2404	37	-0.09729088735510061
2404	38	0.21615130276660466
2404	39	0.45029310132792943
2404	40	0.21998750846785953
2404	41	0.11939606311938888
2404	42	-0.9499931802705817
2404	43	-0.1712137118047039
2404	44	0.5847893182347548
2404	45	1.438521469658692
2404	46	0.9869730061077036
2404	47	-0.17179412914298098
2404	48	0.2829478766554132
2404	49	-0.019508523297666613
2404	50	0.2903478891905422
2404	51	0.08784407243615533
2404	52	0.1737509699680473
2404	53	0.20424417143286178
2404	54	-0.4728880245726188
2404	55	0.44742476917277685
2404	56	0.33764260524039
2404	57	-0.8018813311247825
2404	58	-0.7868126293495123
2404	59	-0.9205261511404998
2404	60	-6.368938253852821
2404	61	-6.521010471343011
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	10.0
19	14.0
20	20.0
21	25.0
22	36.0
23	58.0
24	63.0
25	101.0
26	147.0
27	183.0
28	249.0
29	308.0
30	366.0
31	464.0
32	580.0
33	660.0
34	850.0
35	1183.0
36	2156.0
37	5328.0
38	16078.0
39	13567.0
40	2856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.224208714077605	77.93007548669051	9.387277623272857	5.458438175959034
2	80.61895722043177	5.472164584345062	6.079201801244978	7.8296763939781915
3	6.946099854323931	79.14845715799231	8.91272679115349	4.99271619653026
4	6.462577526651511	5.579711744322069	79.63449356611562	8.323217162910808
5	6.775994879378462	5.131657359789878	9.214911603063545	78.87743615776812
6	80.0737192928245	6.297040192464741	5.968172688547024	7.661067826163727
7	80.67406802480853	6.650186505396517	7.9678636855232075	4.707881784271746
8	4.944048381044872	87.73478711898824	2.308694020791489	5.012470479175404
9	12.931776546670493	79.23499679961154	6.016730306575143	1.8164963471428257
10	77.48030105723177	13.547575429845276	6.747301741452756	2.224821771470192
11	4.793961198048867	76.76738693800075	15.1301123446708	3.3085395192795817
12	77.77826825876795	4.209062617255611	6.343390646037037	11.669278477939391
13	14.403955238704835	4.774096717946454	1.8341536627894146	78.9877943805593
14	13.249608228309093	78.82887853974	2.472024190522436	5.449489041428477
15	5.186836471185468	14.185445957578299	5.559847264219657	75.06787030701658
16	6.453748868828216	85.52100116979716	2.004105325887832	6.02114463548679
17	12.761824883572077	6.961396693667646	2.443331052596729	77.83344737016355
18	78.58388328514357	11.523605623855033	5.85560730130002	4.036903789701371
19	11.271988875891143	5.694484296024896	75.575518131856	7.458008696227957
20	7.30130001986448	6.354426468316154	3.9530315403800738	82.39124197143929
21	10.32070099543117	6.583971571721809	79.24161829297901	3.8537091398680112
22	78.81563555300505	7.469044518507075	5.537775619661421	8.177544308826452
23	8.000971152360563	80.00529719469398	3.7279007658860666	8.265830887059394
24	11.74211490498157	79.9611539055775	3.365925795130995	4.93080539430993
25	9.360584457147903	8.221687597942923	6.806895181759994	75.61083276314918
26	7.638996181605491	9.292162359017372	78.49780387136646	4.571037588010682
27	11.042443772485488	7.8045335157922615	3.1474165140044583	78.0056061977178
28	6.822345332950758	9.053788597788422	5.765113558611252	78.35875251064957
29	9.39589908844108	79.46674906747302	5.306023351799943	5.8313284922859605
30	80.6519963802503	9.283333701194076	3.566777760610943	6.497892157944689
31	6.656807998763988	9.841746308517447	77.08301145518352	6.418434237535038
32	5.979208510826141	8.426953892334518	7.274814046394597	78.31902355044474
33	9.744631072461209	78.54636148939458	4.326042333414263	7.382965104729953
34	5.87988611031408	7.795704857968968	7.703003950824376	78.62140508089259
35	7.137969850133534	6.330147659302095	7.464630189595427	79.06725230096895
36	8.4534398658044	78.26605160350498	5.968172688547024	7.312335842143598
37	6.18668196967356	11.104244377248548	75.65497605226565	7.054097600812237
38	7.945792040964972	78.62802657426005	5.716555940583133	7.709625444191846
39	4.511444147703445	12.134990178118171	4.785132540225572	78.56843313395281
40	7.3410289800693045	8.409296576687929	75.00606970225351	9.243604740989252
41	5.780563709802018	10.35822279118017	5.142693182068996	78.71852031694881
42	77.51782285298077	7.923720396406736	4.7696823890348075	9.788774361577682
43	4.356942635795793	80.84401968790694	4.997020327984638	9.802017348312623
44	4.239962919637142	7.837640982629615	78.44262475997087	9.479771337762378
45	4.9374268876774	9.63427284967003	7.073962080914649	78.35433818173793
46	7.204184783808241	7.382965104729953	75.7807844262476	9.632065685214206
47	75.03476284017923	9.943275873485332	5.085306906217582	9.936654380117863
48	75.35700885072947	6.901803253360408	8.294524024985101	9.446663870925022
49	75.69911934138213	7.458008696227957	5.142693182068996	11.700178780320922
50	78.7074844946697	6.886353102169643	5.22435826693447	9.18180413622619
51	77.84669035689849	7.38075794027413	5.590747566601188	9.18180413622619
52	75.33714437062706	10.020526629439159	5.566468757587128	9.075860242346657
53	75.91762862250866	9.691659125521443	5.471560685986713	8.919151565983181
54	78.65451254772992	6.1624031606595	6.0674950890590855	9.115589202551481
55	76.74972962235415	9.729180921270444	5.7827708742578405	7.738318582117554
56	80.2127706535414	5.663583993643367	5.502460988368243	8.621184364446995
57	77.57300196437636	5.385481272209593	6.157988831747853	10.883527931666189
58	78.04974948683426	8.42253956342287	5.983622839737788	7.544088110005076
59	78.9326152691637	5.102964221864171	7.025404462886529	8.939016046085593
60	80.04944048381046	7.14238417904518	6.420641401990862	6.387533935153508
61	80.91906327940495	4.288520537665262	6.773787714922638	8.018628468007151
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	3.0
21	5.0
22	6.5
23	8.0
24	8.5
25	8.0
26	7.0
27	5.0
28	3.0
29	4.5
30	7.5
31	9.0
32	8.0
33	7.0
34	6.5
35	37.5
36	69.0
37	40.5
38	12.0
39	12.0
40	8.5
41	5.0
42	27.5
43	74.0
44	98.0
45	144.5
46	191.0
47	982.5
48	1705.0
49	1636.0
50	2129.5
51	2623.0
52	2677.5
53	2146.5
54	1561.0
55	1207.0
56	853.0
57	710.5
58	595.5
59	623.0
60	1460.5
61	15688.0
62	29078.0
63	14998.0
64	918.0
65	490.5
66	43.5
67	24.0
68	20.5
69	17.0
70	15.0
71	9.0
72	5.0
73	5.0
74	5.0
75	4.0
76	5.0
77	7.0
78	7.0
79	7.0
80	3.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0022071644558236034
2	0.011035822279118018
3	0.0022071644558236034
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	45307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.642483501445692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.72528717036079	7.875162778378616
2	19.689370651998058	5.372238285474651
3	10.483740495065524	4.290727702121085
4	5.419834978158874	2.9576003708036285
5	2.3135414981394598	1.5781225859138763
6	1.1325028312570782	0.9270090714459134
7	0.4853583562530335	0.4635045357229567
8	0.3235722375020223	0.35314631293177656
9	0.27503640187671896	0.33769616174101136
>10	1.7796473062611229	4.9285982298541064
>50	0.1779647306261123	1.6619948352351734
>100	0.16178611875101115	4.297349195488556
>500	0.0	0.0
>1k	0.016178611875101116	2.531617630829673
>5k	0.0	0.0
>10k+	0.016178611875101116	62.42523230405897
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGGG	28283	62.42523230405897	TruSeq Adapter, Index 22 (97% over 38bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC	1147	2.531617630829673	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGGT	390	0.8607941377712054	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATTTGGGGGGGGGGGGGGG	328	0.7239499415101419	TruSeq Adapter, Index 22 (97% over 38bp)
GGAGGAAAATCCGTTGGCTTTATAGACCGTGAGGGTTCAAGTCCCTCTATCCCCACCTAGG	308	0.6798066523936699	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGTG	194	0.42818990442977906	TruSeq Adapter, Index 22 (97% over 38bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGGG	162	0.3575606418434238	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGTGG	122	0.2692740636104796	TruSeq Adapter, Index 22 (97% over 38bp)
TGGTTGAGGAGTTACTCGGAATGCTGCCAAAATATCCGAGATCGGAAGAGCACACGTCTGA	114	0.2516167479638908	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGGG	113	0.24940958350806716	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATGTGGGGGGGGGGGGGGG	112	0.24720241905224358	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCGGGGGGGGGGGGGGGG	104	0.22954510340565476	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCT	99	0.21850928112653675	TruSeq Adapter, Index 22 (97% over 38bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGG	83	0.1831946498333591	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATC	82	0.18098748537753548	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCATCTGGGGGGGGGGGGGGGG	72	0.15891584081929944	TruSeq Adapter, Index 22 (97% over 38bp)
GATTAACTTATTATACTCCTGAATATCAGACCAAGGATACAGATATCTTGGCAGATCGGAA	68	0.15008718299600504	No Hit
AGATCGGAAGAGCACACGTCTGAACTCAGTCACCATACTCGATCTGGGGGGGGGGGGGGGG	61	0.1346370318052398	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGCTCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGGG	60	0.13242986734941622	TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGG	59	0.1302227028935926	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGG	58	0.128015538437769	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGG	57	0.1258083739819454	TruSeq Adapter, Index 22 (97% over 38bp)
CCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAAGATCGGAAGAG	54	0.11918688061447458	No Hit
AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGG	48	0.10594389387953297	TruSeq Adapter, Index 22 (97% over 38bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	71.18105370031121	0.0	0.0	0.0	0.0
2	71.54965016443376	0.0	0.0	0.0	0.0
3	71.73946630763459	0.0	0.0	0.0	0.0
4	71.83216721477918	0.0	0.0	0.0	0.0
5	72.06171231818483	0.0	0.0	0.0	0.0
6	72.56715297856843	0.0	0.0	0.0	0.0
7	73.28889575562275	0.0	0.0	0.0	0.0
8	78.76487077052111	0.0	0.0	0.0	0.0
9	79.5020636987662	0.0	0.0	0.0	0.0
10	80.06709779945703	0.0	0.0	0.0	0.0
11	80.4290727702121	0.0	0.0	0.0	0.0
12	81.16847286291302	0.0	0.0	0.0	0.0
13	81.9520162447304	0.0	0.0	0.0	0.0
14	82.88343964508796	0.0	0.0	0.0	0.0
15	84.14593771381905	0.0	0.0	0.0	0.0
16	85.62032357030922	0.0	0.0	0.0	0.0
17	86.39283112984748	0.0	0.0	0.0	0.0
18	86.83647118546803	0.0	0.0	0.0	0.0
19	87.66636502085771	0.0	0.0	0.0	0.0
20	87.96874655130554	0.0	0.0	0.0	0.0
21	88.20049881916701	0.0	0.0	0.0	0.0
22	88.45652989604255	0.0	0.0	0.0	0.0
23	88.62206723022932	0.0	0.0	0.0	0.0
24	88.89354845829563	0.0	0.0	0.0	0.0
25	89.1054362460547	0.0	0.0	0.0	0.0
26	89.29745955371135	0.0	0.0	0.0	0.0
27	89.50493301255877	0.0	0.0	0.0	0.0
28	89.66164168892224	0.0	0.0	0.0	0.0
29	89.88897962787208	0.0	0.0	0.0	0.0
30	90.1538393625709	0.0	0.0	0.0	0.0
31	90.38338446597656	0.0	0.0	0.0	0.0
32	90.63279404948463	0.0	0.0	0.0	0.0
33	90.81598869931798	0.0	0.0	0.0	0.0
34	90.97490454013729	0.0	0.0	0.0	0.0
35	91.12499172313329	0.0	0.0	0.0	0.0
36	91.22652128810118	0.0	0.0	0.0	0.0
37	91.33025801752488	0.0	0.0	0.0	0.0
38	91.44723773368354	0.0	0.0	0.0	0.0
39	91.7606550864105	0.0	0.0	0.0	0.0
40	91.81583419780608	0.0	0.0	0.0	0.0
41	92.00785750546274	0.0	0.0	0.0	0.0
42	92.09393691923985	0.0	0.0	0.0	0.0
43	92.2020879775752	0.0	0.0	0.0	0.0
44	92.4250115876134	0.0	0.0	0.0	0.0
45	95.16189551283466	0.0	0.0	0.0	0.0
46	95.19941730858366	0.0	0.0	0.0	0.0
47	95.23031761096519	0.0	0.0	0.0	0.0
48	95.26121791334673	0.0	0.0	0.0	0.0
49	95.2965325446399	0.0	0.0	0.0	0.0
50	95.42896241198932	0.0	0.0	0.0	0.0
>>END_MODULE