FastQCFastQC Report
Mon 8 Jul 2024
AM-AL-LC2_S109_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-LC2_S109_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45307
Sequences flagged as poor quality0
Sequence length61
%GC59

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGGG2828362.42523230405897TruSeq Adapter, Index 22 (97% over 38bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC11472.531617630829673No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGGT3900.8607941377712054TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATTTGGGGGGGGGGGGGGG3280.7239499415101419TruSeq Adapter, Index 22 (97% over 38bp)
GGAGGAAAATCCGTTGGCTTTATAGACCGTGAGGGTTCAAGTCCCTCTATCCCCACCTAGG3080.6798066523936699No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGTG1940.42818990442977906TruSeq Adapter, Index 22 (97% over 38bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGGG1620.3575606418434238TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGTGG1220.2692740636104796TruSeq Adapter, Index 22 (97% over 38bp)
TGGTTGAGGAGTTACTCGGAATGCTGCCAAAATATCCGAGATCGGAAGAGCACACGTCTGA1140.2516167479638908Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGGG1130.24940958350806716TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATGTGGGGGGGGGGGGGGG1120.24720241905224358TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCGGGGGGGGGGGGGGGG1040.22954510340565476TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCT990.21850928112653675TruSeq Adapter, Index 22 (97% over 38bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGG830.1831946498333591TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATC820.18098748537753548TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCATCTGGGGGGGGGGGGGGGG720.15891584081929944TruSeq Adapter, Index 22 (97% over 38bp)
GATTAACTTATTATACTCCTGAATATCAGACCAAGGATACAGATATCTTGGCAGATCGGAA680.15008718299600504No Hit
AGATCGGAAGAGCACACGTCTGAACTCAGTCACCATACTCGATCTGGGGGGGGGGGGGGGG610.1346370318052398Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGCTCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGGGGGGGGGGG600.13242986734941622TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGG590.1302227028935926TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGG580.128015538437769TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGG570.1258083739819454TruSeq Adapter, Index 22 (97% over 38bp)
CCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAAGATCGGAAGAG540.11918688061447458No Hit
AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTCGATCTGGGGGG480.10594389387953297TruSeq Adapter, Index 22 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)