##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-LC1_S46_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 162664 Sequences flagged as poor quality 0 Sequence length 61 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.386170265086314 40.0 40.0 40.0 40.0 40.0 2 39.4567697831112 40.0 40.0 40.0 40.0 40.0 3 39.33416121575763 40.0 40.0 40.0 40.0 40.0 4 39.5287217823243 40.0 40.0 40.0 40.0 40.0 5 39.329587370284756 40.0 40.0 40.0 40.0 40.0 6 39.3479811144445 40.0 40.0 40.0 40.0 40.0 7 39.20363448581124 40.0 40.0 40.0 40.0 40.0 8 38.945384350563124 40.0 40.0 40.0 40.0 40.0 9 39.21799537697339 40.0 40.0 40.0 40.0 40.0 10 39.4102198396695 40.0 40.0 40.0 40.0 40.0 11 39.380760340333445 40.0 40.0 40.0 40.0 40.0 12 39.42962179707864 40.0 40.0 40.0 40.0 40.0 13 38.334112034623516 40.0 40.0 40.0 40.0 40.0 14 39.39612944474499 40.0 40.0 40.0 40.0 40.0 15 39.44395809767373 40.0 40.0 40.0 40.0 40.0 16 39.16856833718585 40.0 40.0 40.0 40.0 40.0 17 39.37085034180888 40.0 40.0 40.0 40.0 40.0 18 39.34424334825161 40.0 40.0 40.0 40.0 40.0 19 39.353710716569125 40.0 40.0 40.0 40.0 40.0 20 24.329611960851818 24.0 12.0 40.0 12.0 40.0 21 25.58198495057296 24.0 12.0 40.0 12.0 40.0 22 26.830472630698864 24.0 12.0 40.0 12.0 40.0 23 36.55825505336153 40.0 40.0 40.0 24.0 40.0 24 38.46468794570403 40.0 40.0 40.0 40.0 40.0 25 25.085452220528204 24.0 12.0 40.0 12.0 40.0 26 37.73538090788374 40.0 40.0 40.0 24.0 40.0 27 38.11501008213249 40.0 40.0 40.0 40.0 40.0 28 21.84923523336448 12.0 12.0 40.0 12.0 40.0 29 34.099763930556236 40.0 24.0 40.0 24.0 40.0 30 20.96963064968278 12.0 12.0 40.0 12.0 40.0 31 27.571312644469582 24.0 24.0 40.0 12.0 40.0 32 22.760758373088084 24.0 12.0 40.0 12.0 40.0 33 35.91511336251414 40.0 40.0 40.0 24.0 40.0 34 19.7737176019279 12.0 12.0 24.0 12.0 40.0 35 19.160773127428318 12.0 12.0 24.0 12.0 40.0 36 22.19881473466778 24.0 12.0 24.0 12.0 40.0 37 25.07940294103182 24.0 12.0 40.0 12.0 40.0 38 20.685093198249152 12.0 12.0 24.0 12.0 40.0 39 30.19869178183249 24.0 24.0 40.0 12.0 40.0 40 21.95121231495598 24.0 12.0 24.0 12.0 40.0 41 18.589632616928146 12.0 12.0 24.0 12.0 40.0 42 20.37040771160183 12.0 12.0 24.0 12.0 40.0 43 23.604977130772635 24.0 12.0 24.0 12.0 40.0 44 17.994368760143608 12.0 12.0 24.0 12.0 40.0 45 25.605296808144395 24.0 24.0 24.0 12.0 40.0 46 18.345300742635125 12.0 12.0 24.0 12.0 40.0 47 16.779250479516058 12.0 12.0 12.0 12.0 40.0 48 23.546402400039344 24.0 24.0 24.0 12.0 40.0 49 16.52793488417843 12.0 12.0 12.0 12.0 40.0 50 23.087345694191708 24.0 24.0 24.0 12.0 40.0 51 16.03304972212659 12.0 12.0 12.0 12.0 24.0 52 23.396928638174398 24.0 24.0 24.0 12.0 24.0 53 15.603108247676191 12.0 12.0 12.0 12.0 24.0 54 16.834530074263512 12.0 12.0 24.0 12.0 24.0 55 21.742610534598928 24.0 12.0 24.0 12.0 24.0 56 15.609956720601977 12.0 12.0 12.0 12.0 24.0 57 20.482811193626127 24.0 12.0 24.0 12.0 24.0 58 20.997713077263562 24.0 12.0 24.0 12.0 24.0 59 17.466384694831063 12.0 12.0 24.0 12.0 24.0 60 15.097218806865687 12.0 12.0 12.0 12.0 24.0 61 19.415986327644717 24.0 12.0 24.0 12.0 24.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.18942639223870827 1101 2 -0.052603164006846725 1101 3 0.004641646162667712 1101 4 -0.021332533370582496 1101 5 -0.010874744090536126 1101 6 -0.1574923283249916 1101 7 -0.12577490290945548 1101 8 0.35091809539939334 1101 9 0.30262433947894607 1101 10 0.06078877852463194 1101 11 -0.21479550183278917 1101 12 0.010150093814480954 1101 13 0.21359138151900225 1101 14 -0.04661408408179568 1101 15 0.18138245716996693 1101 16 0.13673761383812888 1101 17 -0.025864028201723954 1101 18 0.26408012145024173 1101 19 0.19078859055201036 1101 20 -1.485962819732972 1101 21 -2.496469890037684 1101 22 -0.12641977367709245 1101 23 -0.011280886409736013 1101 24 -0.16767372906004852 1101 25 -0.7944219019272438 1101 26 -0.14931469255743934 1101 27 -0.39989481666270876 1101 28 -0.8438716049001336 1101 29 0.0018253126946348175 1101 30 -0.6940902403317963 1101 31 -0.9470380919949157 1101 32 -0.08352964472308955 1101 33 -0.5080219373497812 1101 34 -1.157320404961247 1101 35 -0.8689203135449226 1101 36 -0.5285395886332864 1101 37 -0.45066544725535707 1101 38 0.011518535657614848 1101 39 0.17398885605095415 1101 40 -0.9758440420566608 1101 41 -0.8728706014871968 1101 42 -0.1381481496223067 1101 43 -0.45159730105809004 1101 44 -0.525195943712319 1101 45 -0.04613396818512072 1101 46 -0.45888629480016974 1101 47 -0.1750218921190836 1101 48 -0.3380497835784908 1101 49 -0.3729154003825599 1101 50 -0.1784603832763878 1101 51 -0.05648976220732038 1101 52 0.28908802618111196 1101 53 0.014559511029096583 1101 54 0.614030683364156 1101 55 -0.19752834253148066 1101 56 -0.0830061959583066 1101 57 0.5798955119260611 1101 58 -0.5628259713798016 1101 59 -0.9977713457038284 1101 60 -0.036154863930311976 1101 61 -1.8537369490388365 1102 1 -0.044242528563898986 1102 2 -0.14854139934620036 1102 3 0.14425807777706723 1102 4 0.0420623698145377 1102 5 -0.0210868294168165 1102 6 0.2880867922668173 1102 7 0.1363865526202943 1102 8 0.5768758628898922 1102 9 -0.005645296484821927 1102 10 0.04414800092970239 1102 11 0.18310671437428283 1102 12 0.06428644976102049 1102 13 0.17452707921667354 1102 14 0.09361753339562995 1102 15 0.08790496528273195 1102 16 -0.1117359562392366 1102 17 -0.028417049533295824 1102 18 0.25780522564734554 1102 19 0.048895971244398595 1102 20 -0.8479228018925831 1102 21 -1.5543435001021244 1102 22 0.3043641269088724 1102 23 -0.11309414674101248 1102 24 0.07477101955962695 1102 25 0.5232446980942882 1102 26 0.02536733324960494 1102 27 0.339004923421264 1102 28 -0.17138117873012604 1102 29 -0.5735272657031132 1102 30 -0.08047310125365215 1102 31 -0.6915206437858785 1102 32 0.10145674428968832 1102 33 -0.12162369678134866 1102 34 -0.1188251495822179 1102 35 -0.09744648201357009 1102 36 -0.3688065792978428 1102 37 0.33215856344887484 1102 38 -0.041510485735475555 1102 39 -0.3750645267023103 1102 40 0.13982627983934393 1102 41 0.07094754957169158 1102 42 0.7740186887837481 1102 43 0.4432212960105488 1102 44 -0.4640157096340971 1102 45 0.20489262322380952 1102 46 0.23516639586899757 1102 47 0.10273452503844993 1102 48 0.27169168456257253 1102 49 -0.007679553502320857 1102 50 0.02455095152313902 1102 51 -0.5333818432272999 1102 52 0.2988694814032691 1102 53 -0.07101284251821838 1102 54 0.9838804242171122 1102 55 -0.1426537635493048 1102 56 0.20865342170367995 1102 57 -0.0923488398987331 1102 58 0.4155348658881586 1102 59 -0.4682808733948818 1102 60 0.3599017946876728 1102 61 -0.9200386054267753 1103 1 0.13789831196203295 1103 2 0.13998001712552366 1103 3 0.200432958796668 1103 4 0.1587357585376168 1103 5 -0.07516442883603247 1103 6 0.08003645750840604 1103 7 0.3598599736092396 1103 8 0.3080688934618081 1103 9 0.22108697745242267 1103 10 0.21331796140400883 1103 11 0.11993013939645891 1103 12 0.2594949679509071 1103 13 -0.4625302733686354 1103 14 0.24904223291971306 1103 15 0.14485422720093766 1103 16 0.07718071714948849 1103 17 0.056022734285370746 1103 18 0.10586524016698462 1103 19 0.2001906393092554 1103 20 0.8171897253317475 1103 21 -0.3288211963189518 1103 22 1.1999679020157537 1103 23 0.08963231933949345 1103 24 0.26504285952091067 1103 25 -0.38581394512584666 1103 26 -0.20407527383274982 1103 27 0.28918641894437513 1103 28 0.3333538378061327 1103 29 -1.0377363485457352 1103 30 0.46401346807763844 1103 31 1.6078098393955393 1103 32 1.1017955359328298 1103 33 0.19106485690099362 1103 34 0.402752651498691 1103 35 0.8435231074032252 1103 36 1.517327162736521 1103 37 -0.22142698144101658 1103 38 0.810502097954128 1103 39 -1.339201011335689 1103 40 -0.02566537136659619 1103 41 0.19797828281360452 1103 42 0.1897483007866505 1103 43 -0.14479193299646909 1103 44 -0.13158044303733973 1103 45 0.127454533363359 1103 46 0.6218728571096221 1103 47 -0.09005405284329626 1103 48 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1104 23 -0.3585744189771347 1104 24 -0.3800161108127824 1104 25 -0.22953652291435844 1104 26 -0.29361357436006585 1104 27 -0.2654808917668916 1104 28 0.9218978944820577 1104 29 -0.8526862338252954 1104 30 0.3279590463089299 1104 31 0.7386233482310267 1104 32 0.6795920983447772 1104 33 -0.997020317997368 1104 34 0.16574423866610033 1104 35 0.01484702158428064 1104 36 -0.03303309971165547 1104 37 -0.8064931972764633 1104 38 0.430692896166903 1104 39 -0.6467081562054702 1104 40 0.8613611552203722 1104 41 0.23142029867320346 1104 42 -0.16702020543240437 1104 43 -0.4482818496731973 1104 44 0.519355434272164 1104 45 -0.8081812594152886 1104 46 0.024924018402540327 1104 47 0.3377525727655275 1104 48 -0.2957163811732535 1104 49 0.21230781698672274 1104 50 0.24734488000268584 1104 51 0.04381799149761889 1104 52 -0.4183464130701502 1104 53 0.3717840484392081 1104 54 -0.04523761493437206 1104 55 -0.7847162718325471 1104 56 0.24861568909069653 1104 57 -0.9371383870294174 1104 58 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-0.32985840709007874 2304 19 -0.160191311636261 2304 20 -0.1034618380458987 2304 21 0.12963227681342104 2304 22 0.9430122757285382 2304 23 0.015563792846322144 2304 24 -0.04358848292432782 2304 25 0.019128949463183886 2304 26 -0.5236408982476561 2304 27 -0.3279639274327124 2304 28 0.258723618746032 2304 29 -1.272261718326675 2304 30 1.0496715845352291 2304 31 -0.8470488013084498 2304 32 0.6472739107690018 2304 33 -0.9568849652296976 2304 34 1.0661115730489605 2304 35 0.4316901173264611 2304 36 -0.11330380524700345 2304 37 -0.8725410050321045 2304 38 1.2096408360181599 2304 39 -1.336916794896819 2304 40 -0.3355698836302139 2304 41 -0.05560718807199905 2304 42 -0.09416856871344237 2304 43 -1.5947451252825573 2304 44 0.022585443500762636 2304 45 -0.41439156287329837 2304 46 -0.045792374120484425 2304 47 0.00917830064855707 2304 48 -0.5876838820327812 2304 49 -0.10711023849343704 2304 50 -0.38589978641064704 2304 51 0.226200417352171 2304 52 -0.5956440624920063 2304 53 -0.3711542647178785 2304 54 0.18433732670832015 2304 55 -0.5949509677412017 2304 56 -0.1635045360147096 2304 57 -0.46486941864188935 2304 58 -1.0725892955507206 2304 59 0.15415811561770099 2304 60 0.13344496472067213 2304 61 0.16782446887427227 2401 1 0.09685310883405407 2401 2 0.17904162307631566 2401 3 0.09820141197233312 2401 4 0.04277161587120304 2401 5 0.15653497989942622 2401 6 0.0839273596636616 2401 7 0.11961583857205227 2401 8 0.43852855396151824 2401 9 0.16516479549552798 2401 10 0.15724319504887063 2401 11 0.2515341828830131 2401 12 0.1449040848686991 2401 13 0.3012309320481137 2401 14 0.008035175891293989 2401 15 -0.0641101061959759 2401 16 0.4115694259040197 2401 17 0.06739027195464331 2401 18 0.0030233557496686103 2401 19 0.16456218231898134 2401 20 -0.9401247892591371 2401 21 -0.46446872635900505 2401 22 -0.5632211119075734 2401 23 0.2807442781417038 2401 24 0.4808734908388601 2401 25 -3.857551582697738E-4 2401 26 0.5375611938384282 2401 27 -0.04932206856278043 2401 28 -1.1536574152932388 2401 29 1.212502064576519 2401 30 -0.9156546608561769 2401 31 0.4288664863533853 2401 32 -1.1016757591272501 2401 33 1.2485510289399002 2401 34 -0.544949046002742 2401 35 -0.6375373541645821 2401 36 -0.030717849660963026 2401 37 0.6431925112158581 2401 38 -1.3596295060893588 2401 39 0.9520826049145512 2401 40 -0.7410573225395858 2401 41 -0.7824527681114404 2401 42 -0.5644763797397658 2401 43 -0.13952777125700777 2401 44 -0.6462476183186681 2401 45 0.22129393828420518 2401 46 -0.7801488861671295 2401 47 -0.9973208848097279 2401 48 0.04253132846194774 2401 49 -0.5712789772398992 2401 50 0.8016334383171255 2401 51 -0.6855890021796842 2401 52 0.43863528244497374 2401 53 -0.8141106224303023 2401 54 -0.5322364536226409 2401 55 0.4624956583412789 2401 56 -0.3977667327050973 2401 57 0.13766852185369416 2401 58 0.25211275366474695 2401 59 0.15681609383942785 2401 60 -0.5671123532935614 2401 61 0.8141418829186868 2402 1 -0.06222825029077228 2402 2 -0.04264931367656288 2402 3 0.13901862562153866 2402 4 0.08134294094546846 2402 5 0.22418693961832759 2402 6 0.34955077486325337 2402 7 0.24987737591900583 2402 8 0.384670696973906 2402 9 0.1340121475204512 2402 10 0.23347300016369843 2402 11 0.12951723004667315 2402 12 0.1829248050020027 2402 13 0.7774317579555259 2402 14 0.18838727326142646 2402 15 0.0822059120609282 2402 16 0.4263343320838331 2402 17 0.3177851136557237 2402 18 0.1493393740065727 2402 19 0.3864269943163734 2402 20 1.2781610906220493 2402 21 2.2822530008017594 2402 22 0.6923899871288661 2402 23 0.8780202326443458 2402 24 0.4434323548539254 2402 25 1.4678168095015884 2402 26 0.6353805084801749 2402 27 0.539115227677172 2402 28 1.214132195660902 2402 29 1.6680696948018365 2402 30 -0.05188591493137906 2402 31 0.9651267196360074 2402 32 0.30135256804799937 2402 33 0.7887234552240443 2402 34 0.09583918843904726 2402 35 0.3314838731048546 2402 36 0.635789431364902 2402 37 1.8109096741514534 2402 38 -0.804938496888461 2402 39 1.3047160903887232 2402 40 -0.25129422756023345 2402 41 0.13605770062724076 2402 42 0.32621404987698455 2402 43 0.6526768219473702 2402 44 -0.2979465243535877 2402 45 0.9175193965179247 2402 46 0.9523585397547727 2402 47 -0.6810997734571238 2402 48 -0.4274534574541562 2402 49 -0.8151679868754869 2402 50 0.27564633406442596 2402 51 -0.5519803663758722 2402 52 0.7866900531469945 2402 53 -0.13112866198691897 2402 54 -1.2518336426395233 2402 55 0.15335996320254353 2402 56 0.2137090334326981 2402 57 -0.9277810181013884 2402 58 0.2806572729721424 2402 59 0.9693206218405876 2402 60 -0.3658082889597214 2402 61 0.7526335151725441 2403 1 0.14800370808566754 2403 2 0.18280163319942488 2403 3 0.16326404867943012 2403 4 0.34514873765576226 2403 5 0.3410357843246672 2403 6 0.12577916464928762 2403 7 0.2664008491470753 2403 8 0.5329771159384151 2403 9 0.12756469426448547 2403 10 0.14136438306744736 2403 11 -0.00880313340975647 2403 12 0.05217645060199061 2403 13 0.09833500117445482 2403 14 0.034413093062610756 2403 15 0.09093246507248409 2403 16 0.15636596958702143 2403 17 0.2642412634765421 2403 18 0.1890137026467542 2403 19 0.1858062395145197 2403 20 0.3852787791350849 2403 21 1.1142293701358703 2403 22 -0.39767943544921636 2403 23 0.42647359356678294 2403 24 0.2211001760645459 2403 25 0.4337652598636943 2403 26 -0.22793348249339118 2403 27 0.038777860937031505 2403 28 0.12497741180052557 2403 29 0.25264378803500875 2403 30 -0.6262640355737261 2403 31 -0.6143193991470746 2403 32 -0.13014698962671645 2403 33 0.7950359617839524 2403 34 0.026326645893352207 2403 35 -2.3009987581801283E-4 2403 36 -0.08136229370369463 2403 37 0.9467410207695366 2403 38 -0.340391992757592 2403 39 1.5534528393728735 2403 40 -0.038123430025262195 2403 41 -0.20186382037196537 2403 42 -0.39459623006244016 2403 43 0.7992889101725211 2403 44 -0.4712480702429609 2403 45 -0.30944595216934445 2403 46 0.07168140722212257 2403 47 -0.17058142820349786 2403 48 -0.22431369765927656 2403 49 -0.24197439834295764 2403 50 -0.016993073369636846 2403 51 -0.1870695061656562 2403 52 0.2294051806716233 2403 53 -0.30799879828308363 2403 54 -0.8950195841926085 2403 55 -0.19058917688712285 2403 56 -0.1726433573729338 2403 57 0.0016718480164961136 2403 58 0.24734043312388465 2403 59 0.7015114064806554 2403 60 -0.3865450979201821 2403 61 1.53374997417124 2404 1 0.1685502614577885 2404 2 0.09525675817214108 2404 3 0.1991941753455464 2404 4 0.24325890631813252 2404 5 0.24593459443086374 2404 6 0.20826642653882033 2404 7 0.28667645895824734 2404 8 0.31978969073013275 2404 9 -0.047570213050235566 2404 10 0.1386097901977834 2404 11 0.13539905069639246 2404 12 0.11131740898957787 2404 13 -0.16773157567943997 2404 14 0.147938445101083 2404 15 0.11912564783144575 2404 16 0.2067765243439652 2404 17 0.19203480448310017 2404 18 0.06952759226736305 2404 19 0.11296757887885889 2404 20 -0.36534025163958717 2404 21 -0.1307111351738719 2404 22 -1.1380929254280652 2404 23 0.1210148334346215 2404 24 0.0969177106286665 2404 25 -0.1251612015381376 2404 26 0.3176113774814695 2404 27 0.4794826152439029 2404 28 -0.5948443675460489 2404 29 1.1769225889592292 2404 30 -1.0053742870244022 2404 31 -0.8069602709832431 2404 32 -0.2391716680693925 2404 33 0.40248389922609107 2404 34 -0.20012196138148042 2404 35 -0.020671286307567982 2404 36 0.1640674152577617 2404 37 -0.11603509706080573 2404 38 -0.9067694020855761 2404 39 1.08449469262683 2404 40 -0.04199190197883951 2404 41 -0.5771507571292211 2404 42 0.4299452355917026 2404 43 0.8480135938835431 2404 44 -0.49406753904305845 2404 45 0.1639827633323918 2404 46 -0.9491586683410276 2404 47 -0.9687962111852322 2404 48 0.4946350413242335 2404 49 -0.4896469178922871 2404 50 -0.022818359930084853 2404 51 -0.48450532640965527 2404 52 0.16457440750406604 2404 53 -0.42123815434055345 2404 54 -0.8490043360910953 2404 55 0.3083082333440075 2404 56 -0.19195561230071867 2404 57 -0.4239955386575964 2404 58 1.1725825889314834 2404 59 1.048921261300066 2404 60 0.0416661477428395 2404 61 1.4925665529066876 >>END_MODULE >>Per sequence quality scores warn #Quality Count 14 6.0 15 61.0 16 158.0 17 229.0 18 313.0 19 436.0 20 702.0 21 1008.0 22 1607.0 23 2707.0 24 6706.0 25 24315.0 26 41350.0 27 25561.0 28 10490.0 29 5873.0 30 6515.0 31 7473.0 32 6778.0 33 3932.0 34 2841.0 35 2453.0 36 2738.0 37 2279.0 38 1853.0 39 3221.0 40 1059.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 4.836964540402302 75.86558796045837 9.823316775684846 9.474130723454483 2 71.2364137117002 4.048222102001672 11.339939999016377 13.375424187281759 3 5.243938425220086 72.28028328333251 9.402203314808439 13.07357497663896 4 4.933482516106821 5.218118329808686 78.32341513795308 11.524984016131413 5 3.193085132543157 4.963605960753455 10.130084099739339 81.71322480696405 6 73.50427875866818 4.401096739290808 10.731323464319086 11.36330103772193 7 72.67557664879753 5.937392416269119 7.733733339890818 13.65329759504254 8 5.423449564746964 73.75571730684109 12.054910736241577 8.765922392170364 9 5.598657355038608 74.98647518811784 7.87943244971229 11.535435007131264 10 72.57106673879899 9.56019770815915 7.401760684601387 10.466974868440467 11 6.158092755618944 76.47236020262628 7.722667584714503 9.64687945704028 12 74.63298578665224 7.421433138248168 6.656666502729553 11.28891457237004 13 6.660355087788325 9.713273988098166 7.894186789947375 75.73218413416613 14 75.15430580829194 10.017582255446811 6.727364383022673 8.100747553238579 15 7.163232184134166 8.311611665765012 74.62253479565238 9.902621354448433 16 7.187207987016181 11.095878620961 6.894580239020312 74.8223331530025 17 76.5024836472729 10.474966802734471 5.9742782668568335 7.048271283135789 18 8.490508041115428 9.52454138592436 73.77907834554665 8.205872227413565 19 77.18364235479271 10.339718683912851 6.470392957261595 6.006246004032853 20 19.54519746225348 11.161043623665961 62.526434859587866 6.767324054492696 21 12.378891457237003 73.92354792701519 5.617100280332465 8.080460335415335 22 78.13406777160282 11.073132346431908 5.067501106575518 5.72529877538976 23 79.85909605075493 10.172502827915212 4.658682929228348 5.30971819210151 24 79.77118477352087 11.35039099001623 4.190232626764373 4.688191609698519 25 18.931662814144495 71.5333448089313 4.570156887817833 4.964835489106379 26 13.31763635469434 77.92627747995869 4.4767127329956224 4.27937343235135 27 12.632789062115773 77.16704372202823 5.11668224069247 5.083484975163527 28 26.843677765209268 62.93156445187626 4.447818816701913 5.776938966212561 29 16.21256086165347 74.34343185953868 5.167707667338809 4.276299611469041 30 54.44597452417253 34.16367481434121 6.223872522500369 5.166478138985886 31 16.481827570943786 6.956056656666503 71.17432252987754 5.387793242512172 32 79.22158559976393 6.763020705257464 10.082747258151773 3.932646436826833 33 13.575837308808342 9.260192790045739 73.03767274873358 4.126297152412334 34 43.71833964491221 10.166969950327054 42.938203904982046 3.176486499778685 35 28.911129690650668 60.559804259086214 6.466704372202823 4.062361678060296 36 15.428121772488074 10.87763733831702 68.17673240544927 5.517508483745635 37 17.434097280283282 8.102591845767963 6.36280922638076 68.100501647568 38 13.870924113510059 10.717183888260463 63.87768651945114 11.534205478778341 39 16.665027295529438 9.657330448040131 3.7371514287119463 69.94049082771848 40 13.7621108542763 18.307062410859196 4.26953720552796 63.66128952933655 41 25.943663010869027 66.24575812718241 4.756430433285792 3.054148428662765 42 75.33873506123051 17.171592976934047 5.650297545861408 1.8393744159740326 43 15.68816701913146 10.478655387793243 71.40178527516845 2.431392317906851 44 63.27829144740078 12.271922490532631 21.772488073574976 2.6772979884916146 45 12.163109231298874 10.442384301381988 73.82764471548714 3.566861751831997 46 14.566222397088477 18.025500418039638 8.188658830472631 59.21961835439925 47 50.716200265578124 36.59445236807161 8.593788422761028 4.095558943589239 48 10.15897801603305 72.84156297644223 12.497540943294153 4.501918064230561 49 11.253258250135248 7.2579058673093 39.10576402891851 42.38307185363694 50 11.106944376137314 6.667117493729405 11.504696798308169 70.72124133182511 51 10.839521959376382 6.4249004082034125 42.59332120198692 40.142256430433285 52 9.394269133115705 8.823149702144924 9.341399088913889 72.44118207582548 53 31.090468696208134 12.951851669699504 14.576673388088329 41.381006246004034 54 69.57593567107658 12.236880932474303 10.800177052082821 7.387006344366301 55 9.522082329218511 9.491344120395416 74.06248463089558 6.924088919490483 56 49.42918795300714 7.907145447950671 36.09425622298863 6.5694103760535585 57 72.81725295397818 7.665588766890854 10.640469193788347 8.876689085342612 58 9.4028180789849 7.535779275070083 75.51578714405154 7.545615501893474 59 8.14009246053214 6.814660896080263 17.562582993163822 67.48266365022377 60 34.06859580851208 11.05352784591456 43.00978095817734 11.868095387396027 61 9.519067028150225 6.795644983800864 9.753293619323387 73.93199436872553 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.5 12 1.5 13 2.0 14 1.5 15 1.0 16 3.5 17 7.5 18 9.0 19 9.0 20 12.0 21 15.0 22 17.0 23 19.0 24 21.0 25 18.5 26 14.0 27 20.0 28 26.0 29 21.0 30 14.0 31 12.0 32 10.5 33 9.0 34 7.0 35 7.5 36 10.0 37 11.0 38 12.0 39 23.0 40 97.0 41 160.0 42 354.5 43 1272.0 44 1995.0 45 3679.5 46 5364.0 47 9060.5 48 18981.0 49 25205.0 50 27778.0 51 30351.0 52 31183.5 53 28795.0 54 25574.0 55 20682.0 56 15790.0 57 11869.0 58 5450.0 59 2952.0 60 2002.5 61 692.5 62 332.0 63 236.5 64 141.0 65 102.0 66 44.5 67 26.0 68 19.0 69 12.0 70 9.0 71 6.5 72 7.0 73 5.5 74 4.0 75 2.5 76 1.5 77 2.0 78 1.0 79 0.0 80 1.5 81 2.0 82 1.0 83 3.5 84 5.0 85 4.0 86 2.5 87 1.0 88 2.0 89 1.5 90 0.0 91 0.5 92 1.0 93 1.0 94 1.5 95 2.0 96 2.5 97 3.0 98 6.5 99 53.5 100 97.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 6.147641764619092E-4 53 0.0 54 0.0 55 0.0 56 6.147641764619092E-4 57 0.0012295283529238183 58 0.0 59 0.0 60 6.147641764619092E-4 61 6.147641764619092E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 61 162664.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.48743422023311 #Duplication Level Percentage of deduplicated Percentage of total 1 88.6827555761105 51.86826833226774 2 5.190355063171393 6.071411006737815 3 1.8709663856713405 3.282840702306595 4 1.0185204650087243 2.38282594796636 5 0.6254073030755324 1.82892342497418 6 0.40993083731001284 1.4385481729208676 7 0.29746263322752214 1.217847833571042 8 0.23439635056444322 1.096739290808046 9 0.1755344867455696 0.9239905572222494 >10 1.2728878050831423 14.447572911031328 >50 0.14084803128087622 5.822431515270742 >100 0.07988395803989994 7.827792258889491 >500 0.0 0.0 >1k 0.001051104711051315 1.7908080460335416 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG 2913 1.7908080460335416 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCAACCCCCGTTGTCTC 436 0.2680371809373924 Illumina Single End PCR Primer 1 (96% over 29bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTGATCTCCAGTGTCGATCTCGGTGGTCGC 372 0.2286922736438302 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTTTCAACCCCCGTTGTCTC 353 0.21701175429105396 Illumina Single End PCR Primer 1 (96% over 29bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCAACCCCCGTTGTCTC 339 0.20840505582058722 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCAACCCCCGTTGTCGC 314 0.1930359514090395 Illumina Single End PCR Primer 1 (96% over 29bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTGATCTCCAGTGTCGATCTCGGTGGTCTC 302 0.18565878129149657 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGATCTCGGTGGTCTC 293 0.18012590370333942 Illumina Single End PCR Primer 1 (96% over 33bp) TCCATGTACCAGTAGAAGATTCGGCAGCTACCGCGGCCCCTGCTTCTTCAGGTGGAACTCC 275 0.16906014852702503 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCAACCCCCGTGGTCTC 268 0.16475679929179166 Illumina Single End PCR Primer 1 (96% over 29bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGATCTCGGTGGTCGC 255 0.15676486499778686 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCAACCCCCGTGGTCGC 251 0.1543058082919392 Illumina Single End PCR Primer 1 (96% over 29bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGACCCCCGTTGTCTC 236 0.14508434564501058 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTTTCAACCCCCGTTGTCTC 233 0.14324005311562485 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGATCTCCGTGGTCTC 229 0.14078099640977723 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGATCTCCGTTGTCTC 225 0.1383219397039296 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCAACCCCCGTGGTCGC 220 0.13524811882162002 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCAACCCCCGTGGTCTC 217 0.1334038262922343 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCAACCCCCGTTGTCGC 201 0.12356759946884376 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTGATCTCCAGTGTCGATCTCCGTGGTCTC 185 0.1137313726454532 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTTTCAACCCCCGTTGTCGC 181 0.11127231593960557 Illumina Single End PCR Primer 1 (96% over 29bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCGACCCCCGTTGTCTC 180 0.11065755176314367 Illumina Single End PCR Primer 1 (96% over 29bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGACCCCCGTGGTCGC 180 0.11065755176314367 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTGATCTCCAGTGTCGACCTCGGTGGTCGC 178 0.10942802341021983 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGACCCCCGTGGTCTC 176 0.10819849505729602 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGACCTCCGTTGTCTC 174 0.10696896670437221 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGACCTCCGTGGTCTC 166 0.10205085329267694 Illumina Single End PCR Primer 1 (96% over 33bp) AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCGACCCCCGTGGTCTC 165 0.10143608911621502 Illumina Single End PCR Primer 1 (96% over 29bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 66.84453843505631 0.0012295283529238183 0.0 0.0 0.0 2 67.12610042787587 0.0012295283529238183 0.0 0.0 0.0 3 67.2465942064624 0.0012295283529238183 0.0 0.0 0.0 4 67.3806127969311 0.0012295283529238183 0.0 0.0 0.0 5 67.52385285004672 0.0012295283529238183 0.0 0.0 0.0 6 67.83676781586584 0.0012295283529238183 0.0 0.0 0.0 7 68.13861702650863 0.0012295283529238183 0.0 0.0 0.0 8 69.12715782225938 0.0012295283529238183 0.0 0.0 0.0 9 69.8531943146609 0.0012295283529238183 0.0 0.0 0.0 10 70.59828849653273 0.0012295283529238183 0.0 0.0 0.0 11 71.42084296463877 0.0012295283529238183 0.0 0.0 0.0 12 72.441966261742 0.0012295283529238183 0.0 0.0 0.0 13 73.81043131854621 0.0012295283529238183 0.0 0.0 0.0 14 75.23299562287906 0.0012295283529238183 0.0 0.0 0.0 15 77.12093640879358 0.0012295283529238183 0.0 0.0 0.0 16 79.41646584370235 0.0012295283529238183 0.0 0.0 0.0 17 80.882678404564 0.0012295283529238183 0.0 0.0 0.0 18 82.84008754241873 0.0012295283529238183 0.0 0.0 0.0 19 84.19502778734078 0.0012295283529238183 0.0 0.0 0.0 20 85.5259922293808 0.0012295283529238183 0.0 0.0 0.0 21 86.67744553189397 0.0012295283529238183 0.0 0.0 0.0 22 87.26331579206216 0.0012295283529238183 0.0 0.0 0.0 23 88.17562582993163 0.0012295283529238183 0.0 0.0 0.0 24 89.11621501991836 0.0012295283529238183 0.0 0.0 0.0 25 89.60802636108788 0.0012295283529238183 0.0 0.0 0.0 26 90.20311808390302 0.0012295283529238183 0.0 0.0 0.0 27 90.65496975360251 0.0024590567058476367 0.0 0.0 0.0 28 91.15907637830128 0.0024590567058476367 0.0 0.0 0.0 29 91.70314267447007 0.0024590567058476367 0.0 0.0 0.0 30 92.29946392563812 0.0024590567058476367 0.0 0.0 0.0 31 92.91607239462942 0.0024590567058476367 0.0 0.0 0.0 32 93.47058968179806 0.0036885850587714555 0.0 0.0 0.0 33 93.89908031279201 0.004918113411695273 0.0 0.0 0.0 34 94.25810259184577 0.004918113411695273 0.0 0.0 0.0 35 94.52675453695963 0.006147641764619092 0.0 0.0 0.0 36 94.71794619583927 0.006147641764619092 0.0 0.0 0.0 37 94.84704667289628 0.006762405941081001 0.0 0.0 0.0 38 94.88085870260169 0.00860669847046673 0.0 0.0 0.0 39 94.90729356218955 0.009836226823390547 0.0 0.0 0.0 40 94.92020360989524 0.009836226823390547 0.0 0.0 0.0 41 94.97368809324743 0.009836226823390547 0.0 0.0 0.0 42 95.01672158559977 0.010450990999852456 0.0 0.0 0.0 43 95.04438597354056 0.011065755176314366 0.0 0.0 0.0 44 95.20852800865588 0.011065755176314366 0.0 0.0 0.0 45 97.20343776127477 0.011065755176314366 0.0 0.0 0.0 46 97.25753700880342 0.011065755176314366 0.0 0.0 0.0 47 97.26061082968573 0.011065755176314366 0.0 0.0 0.0 48 97.26737323562682 0.011065755176314366 0.0 0.0 0.0 49 97.26860276397974 0.011065755176314366 0.0 0.0 0.0 50 97.27044705650913 0.011065755176314366 0.0 0.0 0.0 >>END_MODULE