FastQCFastQC Report
Mon 8 Jul 2024
AM-AL-LC1_S46_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-LC1_S46_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162664
Sequences flagged as poor quality0
Sequence length61
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG29131.7908080460335416No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCAACCCCCGTTGTCTC4360.2680371809373924Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTGATCTCCAGTGTCGATCTCGGTGGTCGC3720.2286922736438302Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTTTCAACCCCCGTTGTCTC3530.21701175429105396Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCAACCCCCGTTGTCTC3390.20840505582058722Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCAACCCCCGTTGTCGC3140.1930359514090395Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTGATCTCCAGTGTCGATCTCGGTGGTCTC3020.18565878129149657Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGATCTCGGTGGTCTC2930.18012590370333942Illumina Single End PCR Primer 1 (96% over 33bp)
TCCATGTACCAGTAGAAGATTCGGCAGCTACCGCGGCCCCTGCTTCTTCAGGTGGAACTCC2750.16906014852702503No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCAACCCCCGTGGTCTC2680.16475679929179166Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGATCTCGGTGGTCGC2550.15676486499778686Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCAACCCCCGTGGTCGC2510.1543058082919392Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGACCCCCGTTGTCTC2360.14508434564501058Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTTTCAACCCCCGTTGTCTC2330.14324005311562485Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGATCTCCGTGGTCTC2290.14078099640977723Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGATCTCCGTTGTCTC2250.1383219397039296Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCAACCCCCGTGGTCGC2200.13524811882162002Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCAACCCCCGTGGTCTC2170.1334038262922343Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCAACCCCCGTTGTCGC2010.12356759946884376Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTGATCTCCAGTGTCGATCTCCGTGGTCTC1850.1137313726454532Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTTTCAACCCCCGTTGTCGC1810.11127231593960557Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCGACCCCCGTTGTCTC1800.11065755176314367Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGACCCCCGTGGTCGC1800.11065755176314367Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTGATCTCCAGTGTCGACCTCGGTGGTCGC1780.10942802341021983Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGACCCCCGTGGTCTC1760.10819849505729602Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGACCTCCGTTGTCTC1740.10696896670437221Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTATCTCCAGTGTCGACCTCCGTGGTCTC1660.10205085329267694Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCTCCAGTGTCGACCCCCGTGGTCTC1650.10143608911621502Illumina Single End PCR Primer 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)