##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-LC1_S46_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162664
Sequences flagged as poor quality	0
Sequence length	61
%GC	58
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.08369399498353	40.0	40.0	40.0	40.0	40.0
2	38.957642748241774	40.0	40.0	40.0	40.0	40.0
3	38.74342202331186	40.0	40.0	40.0	40.0	40.0
4	39.082673486450595	40.0	40.0	40.0	40.0	40.0
5	38.94476958638666	40.0	40.0	40.0	40.0	40.0
6	38.83809570648699	40.0	40.0	40.0	40.0	40.0
7	39.048836866178135	40.0	40.0	40.0	40.0	40.0
8	38.689052279545564	40.0	40.0	40.0	40.0	40.0
9	39.10394432695618	40.0	40.0	40.0	40.0	40.0
10	39.16057640289185	40.0	40.0	40.0	40.0	40.0
11	39.26904539418679	40.0	40.0	40.0	40.0	40.0
12	39.27637338317022	40.0	40.0	40.0	40.0	40.0
13	38.8650961491172	40.0	40.0	40.0	40.0	40.0
14	39.23661043623666	40.0	40.0	40.0	40.0	40.0
15	39.47236020262627	40.0	40.0	40.0	40.0	40.0
16	39.35211232971032	40.0	40.0	40.0	40.0	40.0
17	39.40886735848129	40.0	40.0	40.0	40.0	40.0
18	39.32373481532484	40.0	40.0	40.0	40.0	40.0
19	39.371317562582995	40.0	40.0	40.0	40.0	40.0
20	36.75977475040575	40.0	40.0	40.0	24.0	40.0
21	39.4973933998918	40.0	40.0	40.0	40.0	40.0
22	39.17525697142576	40.0	40.0	40.0	40.0	40.0
23	39.20815915015	40.0	40.0	40.0	40.0	40.0
24	39.35366153543501	40.0	40.0	40.0	40.0	40.0
25	39.58496040918703	40.0	40.0	40.0	40.0	40.0
26	39.48792603157429	40.0	40.0	40.0	40.0	40.0
27	39.37360448531943	40.0	40.0	40.0	40.0	40.0
28	39.28775881571829	40.0	40.0	40.0	40.0	40.0
29	39.268627354546794	40.0	40.0	40.0	40.0	40.0
30	39.2895293365465	40.0	40.0	40.0	40.0	40.0
31	39.37704716470762	40.0	40.0	40.0	40.0	40.0
32	39.46109772291349	40.0	40.0	40.0	40.0	40.0
33	39.194191708060785	40.0	40.0	40.0	40.0	40.0
34	39.337505532877586	40.0	40.0	40.0	40.0	40.0
35	39.19677371760193	40.0	40.0	40.0	40.0	40.0
36	39.246372891358874	40.0	40.0	40.0	40.0	40.0
37	39.18415875670093	40.0	40.0	40.0	40.0	40.0
38	39.38526041410515	40.0	40.0	40.0	40.0	40.0
39	39.23574976638961	40.0	40.0	40.0	40.0	40.0
40	39.168125706978806	40.0	40.0	40.0	40.0	40.0
41	39.30135739930163	40.0	40.0	40.0	40.0	40.0
42	39.209905080411154	40.0	40.0	40.0	40.0	40.0
43	38.72429056214036	40.0	40.0	40.0	40.0	40.0
44	38.8595878620961	40.0	40.0	40.0	40.0	40.0
45	27.443761373137264	24.0	24.0	40.0	12.0	40.0
46	31.891727733241527	40.0	24.0	40.0	24.0	40.0
47	38.26702896768799	40.0	40.0	40.0	40.0	40.0
48	38.68056853391039	40.0	40.0	40.0	40.0	40.0
49	38.533271037230115	40.0	40.0	40.0	40.0	40.0
50	38.134067771602815	40.0	40.0	40.0	40.0	40.0
51	37.463483007918164	40.0	40.0	40.0	24.0	40.0
52	37.10200167215856	40.0	40.0	40.0	24.0	40.0
53	35.517016672404466	40.0	40.0	40.0	24.0	40.0
54	32.60185412875621	40.0	24.0	40.0	24.0	40.0
55	32.20965917474057	40.0	24.0	40.0	24.0	40.0
56	31.481876752077902	40.0	24.0	40.0	12.0	40.0
57	29.359686224364335	24.0	24.0	40.0	12.0	40.0
58	27.109919834751388	24.0	24.0	40.0	12.0	40.0
59	24.605173855309104	24.0	12.0	40.0	12.0	40.0
60	23.57055033689077	24.0	12.0	40.0	12.0	40.0
61	22.85233364481385	24.0	12.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3057177821954369
1101	2	0.04150494544515482
1101	3	0.3403271194869859
1101	4	-0.04286119036139269
1101	5	0.11447112378978375
1101	6	-0.14529476389141394
1101	7	-0.12419398253945957
1101	8	0.16739784084730047
1101	9	-0.1956195613904015
1101	10	0.06354825757062343
1101	11	0.0613100610120938
1101	12	-0.10715375283690065
1101	13	-0.3403888001586495
1101	14	0.11018084407813689
1101	15	-0.041266819929674625
1101	16	-0.12262247750739164
1101	17	0.08112088889438951
1101	18	-0.1931553924791487
1101	19	-0.15867875945441767
1101	20	-1.0534542185965776
1101	21	-0.04929517417879481
1101	22	-0.21091720850706253
1101	23	0.2939488319942285
1101	24	0.13553079247952837
1101	25	0.028720360072519213
1101	26	0.07960585871730075
1101	27	-0.08951949746901988
1101	28	0.010558529633975411
1101	29	-0.11548527402795372
1101	30	0.09792817356881045
1101	31	-0.038959820968010206
1101	32	-0.006248247694777831
1101	33	-0.1868154001900777
1101	34	-0.34014357989180866
1101	35	-0.02154303934368329
1101	36	0.3461305940073842
1101	37	0.27166275647061155
1101	38	0.22878229610521572
1101	39	0.0706339974479917
1101	40	0.17803844385017698
1101	41	0.15790243134362925
1101	42	0.03133358888146631
1101	43	-0.0033380987916444838
1101	44	0.15032061619695725
1101	45	-0.7501422321193267
1101	46	-0.21728895512379864
1101	47	0.18687362910512206
1101	48	0.21635480523873696
1101	49	0.020319750704182127
1101	50	-0.06745126116170042
1101	51	-0.35572070073487083
1101	52	-0.4825297140302993
1101	53	-0.432551748148974
1101	54	-0.27408908290150436
1101	55	-0.9703414750330843
1101	56	0.24472437304642014
1101	57	-1.065806443328654
1101	58	-0.913779664200117
1101	59	-1.879697380478909
1101	60	-0.33228744852664605
1101	61	-0.26202955992721755
1102	1	0.1453080684106567
1102	2	0.04959463954398302
1102	3	0.056418843699162835
1102	4	-0.113576805316562
1102	5	-0.335691131481596
1102	6	-0.28429600964278734
1102	7	-0.014567861747714517
1102	8	-0.35686355162589933
1102	9	0.03147629994671064
1102	10	-0.2728661316267136
1102	11	0.027013449148235225
1102	12	-0.026072256089697987
1102	13	-0.4105815071302388
1102	14	-0.10618002394911485
1102	15	-0.0265331185101374
1102	16	-0.13541834345839732
1102	17	0.09634673900435331
1102	18	-0.3263738911180525
1102	19	0.2241514031084435
1102	20	-0.11827942891324739
1102	21	0.028556596787502997
1102	22	-0.15625794457694298
1102	23	0.07238964342437981
1102	24	0.0633079721599401
1102	25	0.05455816631972965
1102	26	0.009228595986797927
1102	27	-0.04525687462409422
1102	28	-0.36452029105473116
1102	29	-0.1600247305112461
1102	30	-0.14869983891946248
1102	31	-0.04954101781211051
1102	32	-0.1886508560828375
1102	33	-0.0444613914851999
1102	34	-0.015971389368750977
1102	35	-0.2554367044672645
1102	36	-0.21362702276258716
1102	37	-0.25868897604657803
1102	38	0.0329071173168245
1102	39	-0.16215233312213684
1102	40	0.10384702876889662
1102	41	0.05467424352737993
1102	42	-0.2339345552565817
1102	43	0.05565822547282551
1102	44	0.10295130474373337
1102	45	-0.7233816470775736
1102	46	-0.5054420133665225
1102	47	-0.1930959191946542
1102	48	-0.4179633381303276
1102	49	-0.2873962179828524
1102	50	-0.2249388420970888
1102	51	-0.42532898113658746
1102	52	-0.12449154174749566
1102	53	-0.6044346782792402
1102	54	-0.2146928878260681
1102	55	-0.7924297234451352
1102	56	-1.8168864911359215
1102	57	-0.9739284346852912
1102	58	-1.1471196620469648
1102	59	-0.5406838310102771
1102	60	0.2171658050375882
1102	61	-0.6716510668249889
1103	1	0.3802036076540247
1103	2	0.12370934471458384
1103	3	0.37507658347491457
1103	4	-0.18846589138577485
1103	5	-0.1943988833570458
1103	6	0.15061566253596936
1103	7	0.19909673452558252
1103	8	0.2657064249813459
1103	9	0.018118229445782674
1103	10	0.1315542926694846
1103	11	0.10199933282977014
1103	12	-0.01923189148536153
1103	13	0.04623869130486469
1103	14	-0.05397384503486791
1103	15	-0.077255067368732
1103	16	0.10712420715869087
1103	17	0.11196342045954566
1103	18	-0.009942496425786373
1103	19	0.15384407068930983
1103	20	-0.6379535436184156
1103	21	0.012294597916813643
1103	22	0.16249659281113082
1103	23	0.11368995801662152
1103	24	0.11586907565271787
1103	25	-0.1851127217983617
1103	26	-0.23637921235393122
1103	27	0.014951240242403685
1103	28	0.04041638717387741
1103	29	0.1383700424176908
1103	30	0.06857853488065047
1103	31	-0.21051544710952186
1103	32	1.0448795200801442E-4
1103	33	0.18888538596095827
1103	34	0.035557206259227314
1103	35	0.17631291237552205
1103	36	0.18753454858532592
1103	37	0.1062057565500183
1103	38	-0.2800718292024982
1103	39	0.22294083457973102
1103	40	-0.19057904205469356
1103	41	0.10749347398636644
1103	42	-0.014946090305386406
1103	43	0.08963418739909912
1103	44	0.32193976969492866
1103	45	-0.03050433805119468
1103	46	0.5961470807691569
1103	47	0.4546414366172371
1103	48	0.23493655700559657
1103	49	0.29151803546555755
1103	50	0.46506680443029325
1103	51	0.1470030346736877
1103	52	0.44706609316417456
1103	53	0.6186354193250168
1103	54	0.7344077010260719
1103	55	0.4216381962128466
1103	56	0.4130401040080649
1103	57	0.5412768569175164
1103	58	0.27354662818454045
1103	59	-0.23761050681893892
1103	60	-0.26275675687255173
1103	61	-0.21673455476562253
1104	1	0.5492653151772657
1104	2	0.14620260237268212
1104	3	0.287663630127895
1104	4	0.21984228395536576
1104	5	-0.07252954390528998
1104	6	0.1524511896462215
1104	7	0.003097861603627905
1104	8	-0.006402418556071154
1104	9	0.23050803743998216
1104	10	0.20270511986426953
1104	11	0.3360934438469556
1104	12	0.09383013806018425
1104	13	0.08154843650471832
1104	14	0.13228385331074577
1104	15	0.02978514516598807
1104	16	0.25279099458371945
1104	17	0.08948626391645575
1104	18	0.18064640185541947
1104	19	0.16971977008149253
1104	20	-0.9636146956531917
1104	21	-0.11247294223534254
1104	22	-0.1148612142170009
1104	23	0.2710783571632618
1104	24	-0.061661818361692156
1104	25	0.11045544629727999
1104	26	0.11282176981408298
1104	27	0.32684593895029224
1104	28	0.1583570545650943
1104	29	0.2124834747728812
1104	30	0.026538523609865194
1104	31	0.16481764470708526
1104	32	0.2624721569130841
1104	33	-0.07433564209523524
1104	34	0.21415077386377845
1104	35	0.21996303514652737
1104	36	0.07229886851447986
1104	37	0.09057157388286896
1104	38	0.28652640772541105
1104	39	0.14742660521934425
1104	40	0.12902809073925425
1104	41	0.19793343695380372
1104	42	0.1704904511751124
1104	43	-0.32065442508731223
1104	44	0.28646901334504804
1104	45	0.6598148665537238
1104	46	0.6711138722194647
1104	47	-0.2366599360028232
1104	48	0.22455133782916903
1104	49	0.13424234412393332
1104	50	0.11060075765655597
1104	51	0.006569109624379621
1104	52	-0.14225081703185793
1104	53	-0.5392601649441957
1104	54	0.03714891585520519
1104	55	0.4631307695735529
1104	56	0.28658194678067517
1104	57	0.26393515100438236
1104	58	-0.43313130926262744
1104	59	-1.0141189800307089
1104	60	-0.41360810332829345
1104	61	0.341797023546178
1201	1	0.26752755131599315
1201	2	0.6193690170342165
1201	3	-0.27189093592721747
1201	4	-0.03589331305867205
1201	5	0.1536321600462287
1201	6	-0.06070943088167979
1201	7	0.08078687537064866
1201	8	0.07139771558209418
1201	9	-0.15400019538893872
1201	10	-0.011360337971503043
1201	11	0.054514423373028364
1201	12	-0.12087029332496968
1201	13	-0.0033157783703288146
1201	14	-0.07420954494862997
1201	15	0.0036877322863446693
1201	16	0.0026114161267827285
1201	17	-0.06601875832746629
1201	18	-0.11215577610385452
1201	19	0.17660923309230014
1201	20	0.25448681280584395
1201	21	0.05977950737355542
1201	22	0.09144169197755758
1201	23	-0.2226135774820861
1201	24	-0.04486676798050837
1201	25	0.12083726382969218
1201	26	0.11245666002319155
1201	27	0.16821367197566417
1201	28	0.1306722078119904
1201	29	0.1221584873731345
1201	30	0.1257213920235074
1201	31	0.07496888746938168
1201	32	0.2892680406970527
1201	33	0.17347865989024314
1201	34	0.04429534940380364
1201	35	0.1203198109095851
1201	36	-0.062077408873719264
1201	37	0.05987060277019651
1201	38	0.24766602650316827
1201	39	-0.16337707286493952
1201	40	0.00947844572915102
1201	41	0.06736700086190694
1201	42	-0.027478427183801557
1201	43	-0.12720973326776175
1201	44	0.01966900223301593
1201	45	-0.6419444070365365
1201	46	-0.6596160695613982
1201	47	-0.23578684737241673
1201	48	0.08144417413695493
1201	49	0.13929038384152648
1201	50	0.09082133159327554
1201	51	-0.350381271161325
1201	52	0.13513737801614667
1201	53	0.07687054436094343
1201	54	-0.176773672931958
1201	55	-0.03814909898692065
1201	56	-0.05858967466396692
1201	57	0.5223058882483578
1201	58	-0.33007510219775327
1201	59	1.536347527098851
1201	60	0.4308908430124738
1201	61	1.1465307513568277
1202	1	-0.18118032091740588
1202	2	0.20951929369417854
1202	3	0.25065056306674904
1202	4	0.20333895555300074
1202	5	0.24348376970417718
1202	6	0.38872152988290765
1202	7	0.04351637738272274
1202	8	0.22536841477726455
1202	9	0.33900470903761715
1202	10	0.05269587430213818
1202	11	0.20713326140119648
1202	12	0.17850407790239586
1202	13	-0.5100750842053401
1202	14	0.09969324680186986
1202	15	0.10495156528433114
1202	16	0.2882785438933837
1202	17	-0.11628913056086532
1202	18	0.22510214643135384
1202	19	-0.20081154155558778
1202	20	0.21080283393260402
1202	21	-0.0749821877974739
1202	22	0.003759347913174338
1202	23	0.08100500903702823
1202	24	0.17237417769353414
1202	25	-0.04302088506366886
1202	26	-0.0549697735784207
1202	27	-0.1469428924106566
1202	28	-0.09383106180571588
1202	29	0.07663534854013676
1202	30	0.001837463452076804
1202	31	-0.13532412057890753
1202	32	-0.14115826715003266
1202	33	-0.19070645077373882
1202	34	-0.5002846304754698
1202	35	-0.25901872027753825
1202	36	0.016424854707771885
1202	37	-0.20702766181732812
1202	38	-0.2180501455290269
1202	39	-0.041568093991699584
1202	40	-0.1137295522587749
1202	41	-0.16447718927894073
1202	42	0.15329370561298106
1202	43	0.038581891480731656
1202	44	-0.04107043438670388
1202	45	-0.2083742360103784
1202	46	-0.5541080212716594
1202	47	0.07932255906621322
1202	48	-0.1708351266678747
1202	49	-0.024457729840563047
1202	50	0.04988823300172385
1202	51	0.3282530346736863
1202	52	0.25436838908254344
1202	53	-0.3031439174096775
1202	54	0.07066407857709578
1202	55	0.4486152370291734
1202	56	-0.9130759674205073
1202	57	-0.7442779900212599
1202	58	0.5012251996131134
1202	59	1.0605081156300393
1202	60	0.9147942635356117
1202	61	1.4746557513568277
1203	1	0.28630151775995216
1203	2	0.5889500301671262
1203	3	0.28750552298659215
1203	4	0.44893953170530665
1203	5	0.4219031083815352
1203	6	0.2968346310853107
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2403	26	0.08222497980649024
2403	27	0.022861870645435545
2403	28	0.061179818271654085
2403	29	-0.2577456959472926
2403	30	-0.14617692242799762
2403	31	0.003608422941404399
2403	32	0.20576508874938781
2403	33	0.11296859758216016
2403	34	0.02153596913768041
2403	35	0.1842235427785539
2403	36	0.3020571080926828
2403	37	0.14329571826004184
2403	38	-0.09391148498744428
2403	39	-0.08764219940756135
2403	40	-0.03457698939610765
2403	41	0.15323618112724802
2403	42	-0.0415201241742551
2403	43	0.3521850104362443
2403	44	-0.03732285218167419
2403	45	1.5475795841056765
2403	46	-0.48677243132968684
2403	47	0.11066322637762482
2403	48	0.26154217023734816
2403	49	0.27639152064299566
2403	50	0.12187807826284569
2403	51	0.5121867870914798
2403	52	0.4421555022353516
2403	53	-0.09480554700348875
2403	54	0.5213259499504019
2403	55	-0.16910115562073003
2403	56	0.285712112850284
2403	57	-0.9128738555918581
2403	58	-0.11440584487431238
2403	59	-1.229944616478278
2403	60	-0.9933295275669032
2403	61	-2.0101423627630943
2404	1	-0.20790089484736995
2404	2	-0.21530439307625215
2404	3	0.052389376296318346
2404	4	-0.00395068452481695
2404	5	0.15338162962635948
2404	6	0.0025651582875738654
2404	7	0.08592274897805652
2404	8	0.16739784084730047
2404	9	0.24796020514656902
2404	10	0.09467666223988402
2404	11	0.39594041120664514
2404	12	0.025141967007456856
2404	13	0.5623349352499147
2404	14	0.14130924874739748
2404	15	0.3711845419380282
2404	16	0.2976109855276263
2404	17	-0.06673903328459829
2404	18	0.20373176705392382
2404	19	0.07478427556503675
2404	20	-0.08847367384949933
2404	21	0.07521844449824755
2404	22	0.0381100288470293
2404	23	0.21612782032107702
2404	24	-0.12906064720918664
2404	25	0.12988767524761613
2404	26	0.0017848470441492736
2404	27	0.08168672821191336
2404	28	-0.02056987503528518
2404	29	-0.3411662078801001
2404	30	0.1368386794053862
2404	31	-0.015613517466064764
2404	32	0.08713696631300394
2404	33	0.1089044441678979
2404	34	0.04117937730663357
2404	35	-0.037107241678313585
2404	36	0.027064446147463173
2404	37	-0.11744230189514582
2404	38	-0.03580914358349929
2404	39	0.05506979511336141
2404	40	0.1157816345116558
2404	41	0.10342772317242321
2404	42	0.12471880288924808
2404	43	0.3390743525702291
2404	44	0.142538515029635
2404	45	-0.03418892472633317
2404	46	-0.18616055045453805
2404	47	-0.0699357094162778
2404	48	0.15409799590021578
2404	49	0.31603959506215773
2404	50	0.11153706568654798
2404	51	0.1578979763079289
2404	52	0.3034625038685377
2404	53	0.13554163706503175
2404	54	-0.9666960867925525
2404	55	-0.6357111248385294
2404	56	-1.288349556914671
2404	57	-1.2525768713442176
2404	58	-2.6414061233440833
2404	59	-1.8330047734750181
2404	60	-3.7408477598106913
2404	61	-3.5772046572034846
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	6.0
17	18.0
18	22.0
19	33.0
20	57.0
21	84.0
22	117.0
23	170.0
24	232.0
25	328.0
26	552.0
27	679.0
28	959.0
29	1091.0
30	1322.0
31	1762.0
32	2335.0
33	2966.0
34	4406.0
35	7977.0
36	27991.0
37	51844.0
38	21553.0
39	28701.0
40	7458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.003049249060942	76.04433706497483	9.57562568008705	6.376988005877182
2	78.30075567060386	6.363250674815695	7.69443607543179	7.641557579148656
3	8.349932374277634	76.57014631747204	8.993606295339973	6.086315012910365
4	7.584960409187036	6.729208675552058	77.58508336202233	8.100747553238579
5	8.125952884473515	6.246618797029459	9.030270988048985	76.59715733044804
6	78.20230659519008	7.008926375842226	7.5068853587763735	7.281881670191314
7	78.93449072935623	7.2357743569566715	8.590099837702256	5.239635075984852
8	5.527959474745487	87.5565583042345	2.235282545615502	4.680199675404515
9	16.45108936212069	76.24735651404121	5.533492352333645	1.7680617715044509
10	75.2791029361137	16.636133379235726	5.5095165494516305	2.575247135198938
11	5.5562386268627355	74.30285742389219	17.049869669994592	3.0910342792504797
12	75.6940687552255	4.852333644813849	5.740667879801308	13.712929720159348
13	17.911154281217726	5.647223724979098	1.6346579452122165	74.80696404859096
14	17.220774111051	76.22030689027689	1.7994147445040083	4.759504254168101
15	6.609944425318448	18.117100280332465	4.262774799586879	71.01018049476221
16	7.108518172429055	85.55427138149805	2.3188904736143217	5.018319972458564
17	15.202503319726555	8.755471401170512	2.6975852063148573	73.34444007278807
18	76.37584222692175	14.508434564501057	5.507057492745783	3.6086657158314073
19	13.988344071214282	7.14540402301677	71.87515369104412	6.991098214724832
20	9.623518418334728	7.514877293070378	3.6590763783012834	79.20252791029361
21	12.286676830767718	8.357718978999655	74.76638961294448	4.5892145772881525
22	77.57955048443417	8.41058869817538	5.865465007623076	8.144395809767373
23	9.819013426449615	78.4039492450696	3.6185019426547975	8.158535385825997
24	13.074189740815424	77.43508090296562	4.000270496237643	5.490458859981311
25	11.206536172724144	10.157748487680125	5.893129395563862	72.74258594403187
26	9.292160527221757	11.304898440958048	73.97272906113214	5.430211970688044
27	12.209216544533517	9.744012196921261	3.159887867014213	74.88688339153101
28	8.495426154527124	11.515762553484484	5.122215118280628	74.86659617370776
29	10.444843358087837	77.2322087247332	4.982663650223775	7.340284266955196
30	77.81070181478384	10.800791816259283	3.3572271676584866	8.031279201298382
31	7.405449269660158	11.950400826243053	73.03890227708652	7.60524762701028
32	7.469384744012197	9.428638174396301	6.384325972556927	76.71765110903458
33	9.490729356218955	76.67953573009395	5.312177248807357	8.517557664879751
34	6.717528156199282	9.41511336251414	7.686396498303251	76.18096198298333
35	7.977794717946196	74.68093739241627	7.300939359661633	10.040328529975902
36	76.34141543303988	9.399744258102592	6.281045590911326	7.977794717946196
37	75.59201790193282	10.594845817144543	4.538803914818276	9.274332366104362
38	8.424113510057541	10.052009049328678	6.144567943736782	75.379309496877
39	6.004401711503467	78.79555402547582	5.084099739339989	10.115944523680716
40	7.797054050066395	76.79265233856293	5.503983671863473	9.906309939507205
41	6.2478483253823835	11.609206708306694	72.96144199085231	9.181502975458613
42	73.87805537795703	10.295455663207594	4.819751143461368	11.006737815374022
43	5.740053115624846	77.40557222249545	6.757487827669307	10.096886834210398
44	6.298258987852259	9.177814390399842	73.57743569566713	10.946490926080756
45	7.154625485663699	9.911842817095362	7.725741405596813	75.20779029164413
46	8.80711159199331	9.471056902572174	71.42084296463877	10.300988540795752
47	73.81903801701667	11.493016278955393	4.276914375645503	10.411031328382432
48	74.02498401613141	9.22883981704618	7.524098755717307	9.2220774111051
49	75.3485712880539	8.611616583878424	4.790242462991197	11.249569665076477
50	77.4467614223184	9.495647469630649	4.267692912998574	8.789898195052379
51	77.73201200019673	8.788668666699454	5.318324890571977	8.160994442531845
52	76.0137461269857	11.745069591304775	4.216052722175774	8.025131559533763
53	75.95657305857473	11.02518074066788	5.54824669256873	7.469999508188659
54	78.00435253036935	9.841144936802243	5.64783848915556	6.5066640436728465
55	76.16866669945409	11.556951753307432	5.622633157920622	6.6517483893178575
56	78.67444548271283	8.439482614469089	6.553386121083952	6.332685781734126
57	76.9512614960901	8.747479466876506	5.930630010328039	8.370629026705355
58	76.35862882998083	10.199552451679535	7.74418433089067	5.697634387448974
59	77.59553435302217	6.534328431613632	8.698298332759553	7.171838882604632
60	76.95679437367826	8.040500663945311	9.303226282398072	5.699478679978361
61	75.61537894063837	4.970983130870998	12.900826243053165	6.512811685437467
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	3.0
18	5.0
19	4.0
20	10.0
21	17.0
22	20.0
23	23.0
24	20.5
25	21.5
26	25.0
27	23.0
28	21.0
29	19.5
30	19.0
31	20.0
32	18.0
33	16.0
34	12.0
35	6.0
36	4.0
37	7.0
38	10.0
39	9.5
40	11.0
41	13.0
42	21.0
43	103.0
44	177.0
45	423.5
46	670.0
47	2994.5
48	5872.0
49	6425.0
50	8655.5
51	10886.0
52	11056.0
53	9661.0
54	8096.0
55	6472.0
56	4848.0
57	4276.5
58	4359.5
59	5014.0
60	9963.5
61	51068.0
62	87223.0
63	45292.5
64	3362.0
65	1897.5
66	238.5
67	44.0
68	34.0
69	24.0
70	20.5
71	13.0
72	9.0
73	10.5
74	12.0
75	12.0
76	8.5
77	5.0
78	3.5
79	2.0
80	1.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.147641764619092E-4
2	0.01659863276447155
3	0.0024590567058476367
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	162664.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.991245758127182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.16546209441796	9.301381989868686
2	20.117638013224663	6.434121870850341
3	10.506689220359835	5.040451482811194
4	4.9900046132554206	3.191855604190233
5	2.3143164693218514	1.8504401711503466
6	1.1802245117638013	1.1323956130428368
7	0.5497462709518685	0.6153789406383712
8	0.35752729509457176	0.4573845472876604
9	0.22681839151161	0.3264397777012738
>10	1.264800861141012	3.9264987950622143
>50	0.1768414577887129	2.027492253971377
>100	0.11917576503152391	4.6334775979934095
>500	0.003844379517145933	0.3620960999360645
>1k	0.019221897585729664	5.279594747454876
>5k	0.003844379517145933	3.6879702945949937
>10k+	0.003844379517145933	51.733020213446125
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGGG	84151	51.733020213446125	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGGT	5999	3.6879702945949937	TruSeq Adapter, Index 13 (97% over 39bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC	2966	1.8233905473860228	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGTG	2196	1.3500221315103527	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGTGG	1312	0.8065705995180248	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGTT	1086	0.6676338956376334	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATTTGGGGGGGGGGGGGGG	1028	0.6319775734028427	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGTGT	589	0.3620960999360645	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATGTGGGGGGGGGGGGGGG	461	0.28340628534894013	TruSeq Adapter, Index 13 (97% over 39bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGGG	452	0.27787340776078295	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGTGGG	419	0.25758618993753996	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATATCTGGGGGGGGGGGGGGGG	387	0.23791373629075885	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGG	374	0.22992180199675408	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCT	351	0.21578222593813012	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCGGGGGGGGGGGGGGGG	324	0.19918359317365858	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGG	317	0.1948802439384252	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGG	296	0.18197019623272512	TruSeq Adapter, Index 13 (97% over 39bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGGG	293	0.18012590370333942	TruSeq Adapter, Index 13 (97% over 39bp)
GGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGCCGAATCTTCTACTGGTACATGGA	276	0.16967491270348695	No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGG	251	0.1543058082919392	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGG	250	0.1536910441154773	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGAT	235	0.14446958146854866	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCTGGGGGGGGGGGGGGGGGG	234	0.14385481729208674	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGG	225	0.1383219397039296	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATC	223	0.13709241135100575	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGG	208	0.12787094870407711	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGTGGGG	201	0.12356759946884376	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGGCAATGATCTGGGGGGGGGGGGGGGG	193	0.11864948605714846	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGG	182	0.11188708011606747	TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTG	172	0.10573943835144838	TruSeq Adapter, Index 13 (97% over 39bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	67.866276496336	0.0	0.0	0.0	0.0
2	68.19517533074313	0.0	0.0	0.0	0.0
3	68.38083411203462	0.0	0.0	0.0	0.0
4	68.63350218856047	0.0	0.0	0.0	0.0
5	68.95502385285005	0.0	0.0	0.0	0.0
6	69.54458269807702	0.0	0.0	0.0	0.0
7	70.31734126788963	0.0	0.0	0.0	0.0
8	77.4111051000836	0.0	0.0	0.0	0.0
9	78.47280283283332	0.0	0.0	0.0	0.0
10	79.22588894899917	0.0	0.0	0.0	0.0
11	79.80376727487337	0.0	0.0	0.0	0.0
12	80.80030000491811	0.0	0.0	0.0	0.0
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50	96.69133920228201	0.0	0.0	0.0	0.0
>>END_MODULE