FastQCFastQC Report
Mon 8 Jul 2024
AM-AL-LC1_S46_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-LC1_S46_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162664
Sequences flagged as poor quality0
Sequence length61
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGGG8415151.733020213446125TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGGT59993.6879702945949937TruSeq Adapter, Index 13 (97% over 39bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC29661.8233905473860228No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGTG21961.3500221315103527TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGTGG13120.8065705995180248TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGTT10860.6676338956376334TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATTTGGGGGGGGGGGGGGG10280.6319775734028427TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGTGT5890.3620960999360645TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATGTGGGGGGGGGGGGGGG4610.28340628534894013TruSeq Adapter, Index 13 (97% over 39bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGGG4520.27787340776078295TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGTGGG4190.25758618993753996TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATATCTGGGGGGGGGGGGGGGG3870.23791373629075885TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGG3740.22992180199675408TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCT3510.21578222593813012TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCGGGGGGGGGGGGGGGG3240.19918359317365858TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGG3170.1948802439384252TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGG2960.18197019623272512TruSeq Adapter, Index 13 (97% over 39bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGGG2930.18012590370333942TruSeq Adapter, Index 13 (97% over 39bp)
GGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGCCGAATCTTCTACTGGTACATGGA2760.16967491270348695No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGGGGG2510.1543058082919392TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGG2500.1536910441154773TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGAT2350.14446958146854866TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCTGGGGGGGGGGGGGGGGGG2340.14385481729208674TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGG2250.1383219397039296TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATC2230.13709241135100575TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGG2080.12787094870407711TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGGGGGGTGGGG2010.12356759946884376TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGGCAATGATCTGGGGGGGGGGGGGGGG1930.11864948605714846Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTGGGGGG1820.11188708011606747TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATGATCTG1720.10573943835144838TruSeq Adapter, Index 13 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)