##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-LC1.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1900 Sequences flagged as poor quality 0 Sequence length 30-90 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.36842105263158 40.0 40.0 40.0 40.0 40.0 2 38.39684210526316 40.0 40.0 40.0 40.0 40.0 3 38.566315789473684 40.0 40.0 40.0 40.0 40.0 4 38.345263157894735 40.0 40.0 40.0 40.0 40.0 5 38.90526315789474 40.0 40.0 40.0 40.0 40.0 6 38.89052631578947 40.0 40.0 40.0 40.0 40.0 7 38.962105263157895 40.0 40.0 40.0 40.0 40.0 8 38.86105263157895 40.0 40.0 40.0 40.0 40.0 9 39.02315789473684 40.0 40.0 40.0 40.0 40.0 10-11 39.089473684210525 40.0 40.0 40.0 40.0 40.0 12-13 39.09157894736842 40.0 40.0 40.0 40.0 40.0 14-15 39.054736842105264 40.0 40.0 40.0 40.0 40.0 16-17 39.207368421052635 40.0 40.0 40.0 40.0 40.0 18-19 39.05157894736842 40.0 40.0 40.0 40.0 40.0 20-21 39.10947368421053 40.0 40.0 40.0 40.0 40.0 22-23 38.7 40.0 40.0 40.0 40.0 40.0 24-25 38.82526315789474 40.0 40.0 40.0 40.0 40.0 26-27 38.34105263157895 40.0 40.0 40.0 40.0 40.0 28-29 38.065263157894734 40.0 40.0 40.0 40.0 40.0 30-31 38.65016712397106 40.0 40.0 40.0 40.0 40.0 32-33 38.584236704180306 40.0 40.0 40.0 40.0 40.0 34-35 38.70180154609052 40.0 40.0 40.0 40.0 40.0 36-37 38.58646435452794 40.0 40.0 40.0 40.0 40.0 38-39 38.982731554160125 40.0 40.0 40.0 40.0 40.0 40-41 38.39136288318144 40.0 40.0 40.0 40.0 40.0 42-43 38.73209615145099 40.0 40.0 40.0 40.0 40.0 44-45 38.92427025923658 40.0 40.0 40.0 40.0 40.0 46-47 39.21816168327796 40.0 40.0 40.0 40.0 40.0 48-49 38.21774193548387 40.0 40.0 40.0 32.0 40.0 50-51 38.87485805132864 40.0 40.0 40.0 40.0 40.0 52-53 38.40571428571428 40.0 40.0 40.0 40.0 40.0 54-55 37.502717391304344 NaN NaN NaN NaN NaN 56-57 35.50457277240658 NaN NaN NaN NaN NaN 58-59 37.06485671191554 NaN NaN NaN NaN NaN 60-61 34.93808049535603 NaN NaN NaN NaN NaN 62-63 39.69230769230769 NaN NaN NaN NaN NaN 64-65 40.0 NaN NaN NaN NaN NaN 66-67 40.0 NaN NaN NaN NaN NaN 68-69 40.0 NaN NaN NaN NaN NaN 70-71 39.61904761904762 NaN NaN NaN NaN NaN 72-73 39.578947368421055 NaN NaN NaN NaN NaN 74-75 40.0 NaN NaN NaN NaN NaN 76-77 40.0 NaN NaN NaN NaN NaN 78-79 40.0 NaN NaN NaN NaN NaN 80-81 40.0 NaN NaN NaN NaN NaN 82-83 40.0 NaN NaN NaN NaN NaN 84-85 40.0 NaN NaN NaN NaN NaN 86-87 40.0 NaN NaN NaN NaN NaN 88-89 40.0 NaN NaN NaN NaN NaN 90 40.0 NaN NaN NaN NaN NaN >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1636655011655037 1101 2 1.64393488455989 1101 3 1.1202752109002105 1101 4 1.9414748445998455 1101 5 1.2454545454545496 1101 6 1.3446074758574795 1101 7 0.5801767676767753 1101 8 1.349522005772009 1101 9 1.0223079004329065 1101 10-11 0.7139475108225142 1101 12-13 0.9539214257964304 1101 14-15 1.2513731060606048 1101 16-17 0.841832126207116 1101 18-19 0.8942155067155042 1101 20-21 1.0453454531579567 1101 22-23 1.2154990842490818 1101 24-25 1.0973595848595892 1101 26-27 2.1414835164835253 1101 28-29 1.7798382173382095 1101 30-31 0.9962958916083977 1101 32-33 1.5611855158730137 1101 34-35 3.1510416666666643 1101 36-37 11.75 1101 38-39 17.020833333333332 1101 40-41 18.729166666666664 1101 42-43 20.9375 1101 44-45 23.0625 1101 46-47 30.5625 1101 48-49 31.5 1101 50-51 31.6875 1101 52-53 31.71875 1101 54-55 32.4375 1101 56-57 32.125 1101 58-59 32.5625 1101 60-61 31.9375 1101 62-63 32.75 1101 64-65 32.5 1101 66-67 32.5 1101 68-69 35.0 1101 70-71 35.0 1101 72-73 36.25 1101 74-75 37.5 1101 76-77 37.5 1101 78-79 37.5 1101 80-81 37.5 1102 1 -4.169667832167832 1102 2 1.64393488455989 1102 3 1.1202752109002105 1102 4 1.9414748445998455 1102 5 1.2454545454545496 1102 6 1.3446074758574795 1102 7 0.5801767676767753 1102 8 -3.9838113275613267 1102 9 1.0223079004329065 1102 10-11 0.7139475108225142 1102 12-13 0.9539214257964304 1102 14-15 1.2513731060606048 1102 16-17 0.841832126207116 1102 18-19 0.8942155067155042 1102 20-21 1.0453454531579567 1102 22-23 1.2154990842490818 1102 24-25 1.0973595848595892 1102 26-27 2.1414835164835253 1102 28-29 1.7798382173382095 1102 30-31 0.9962958916083977 1102 32-33 1.5611855158730137 1102 34-35 3.1510416666666643 1102 36-37 -28.25 1102 38-39 -22.979166666666668 1102 40-41 -21.270833333333336 1102 42-43 -19.0625 1102 44-45 -16.9375 1102 46-47 -9.4375 1102 48-49 -8.5 1102 50-51 -8.3125 1102 52-53 -8.28125 1102 54-55 -7.5625 1102 56-57 -7.875 1102 58-59 -7.4375 1102 60-61 -8.0625 1102 62-63 -7.25 1102 64-65 -7.5 1102 66-67 -7.5 1102 68-69 -5.0 1102 70-71 -5.0 1102 72-73 -3.75 1102 74-75 -2.5 1102 76-77 -2.5 1102 78-79 -2.5 1102 80-81 -2.5 1103 1 1.1636655011655037 1103 2 1.64393488455989 1103 3 1.1202752109002105 1103 4 1.9414748445998455 1103 5 1.2454545454545496 1103 6 1.3446074758574795 1103 7 0.5801767676767753 1103 8 1.349522005772009 1103 9 -2.9776920995670935 1103 10-11 0.7139475108225142 1103 12-13 0.9539214257964304 1103 14-15 -2.748626893939395 1103 16-17 0.841832126207116 1103 18-19 0.8942155067155042 1103 20-21 1.0453454531579567 1103 22-23 1.2154990842490818 1103 24-25 1.0973595848595892 1103 26-27 2.1414835164835253 1103 28-29 1.7798382173382095 1103 30-31 0.9962958916083977 1103 32-33 1.5611855158730137 1103 34-35 3.1510416666666643 1103 36-37 11.75 1103 38-39 17.020833333333332 1103 40-41 18.729166666666664 1103 42-43 20.9375 1103 44-45 23.0625 1103 46-47 -9.4375 1103 48-49 -8.5 1103 50-51 -8.3125 1103 52-53 -8.28125 1103 54-55 -7.5625 1103 56-57 -7.875 1103 58-59 -7.4375 1103 60-61 -8.0625 1103 62-63 -7.25 1103 64-65 -7.5 1103 66-67 -7.5 1103 68-69 -5.0 1103 70-71 -5.0 1103 72-73 -3.75 1103 74-75 -2.5 1103 76-77 -2.5 1103 78-79 -2.5 1103 80-81 -2.5 1104 1 1.1636655011655037 1104 2 1.64393488455989 1104 3 1.1202752109002105 1104 4 1.9414748445998455 1104 5 1.2454545454545496 1104 6 1.3446074758574795 1104 7 0.5801767676767753 1104 8 1.349522005772009 1104 9 1.0223079004329065 1104 10-11 0.7139475108225142 1104 12-13 0.9539214257964304 1104 14-15 -1.4152935606060666 1104 16-17 0.841832126207116 1104 18-19 0.8942155067155042 1104 20-21 1.0453454531579567 1104 22-23 1.2154990842490818 1104 24-25 1.0973595848595892 1104 26-27 -0.5251831501831461 1104 28-29 1.7798382173382095 1104 30-31 0.9962958916083977 1104 32-33 1.5611855158730137 1104 34-35 3.1510416666666643 1104 36-37 -28.25 1104 38-39 -22.979166666666668 1104 40-41 -21.270833333333336 1104 42-43 -19.0625 1104 44-45 -16.9375 1104 46-47 -9.4375 1104 48-49 -8.5 1104 50-51 -8.3125 1104 52-53 -8.28125 1104 54-55 -7.5625 1104 56-57 -7.875 1104 58-59 -7.4375 1104 60-61 -8.0625 1104 62-63 -7.25 1104 64-65 -7.5 1104 66-67 -7.5 1104 68-69 -5.0 1104 70-71 -5.0 1104 72-73 -3.75 1104 74-75 -2.5 1104 76-77 -2.5 1104 78-79 -2.5 1104 80-81 -2.5 1201 1 1.1636655011655037 1201 2 1.64393488455989 1201 3 1.1202752109002105 1201 4 1.9414748445998455 1201 5 1.2454545454545496 1201 6 1.3446074758574795 1201 7 0.5801767676767753 1201 8 1.349522005772009 1201 9 1.0223079004329065 1201 10-11 0.7139475108225142 1201 12-13 -3.0460785742035696 1201 14-15 1.2513731060606048 1201 16-17 0.841832126207116 1201 18-19 -3.105784493284496 1201 20-21 -2.9546545468420433 1201 22-23 1.2154990842490818 1201 24-25 1.0973595848595892 1201 26-27 -1.8585164835164747 1201 28-29 1.7798382173382095 1201 30-31 0.9962958916083977 1201 32-33 1.5611855158730137 1201 34-35 -36.848958333333336 1201 36-37 -28.25 1201 38-39 -22.979166666666668 1201 40-41 -21.270833333333336 1201 42-43 -19.0625 1201 44-45 -16.9375 1201 46-47 -9.4375 1201 48-49 -8.5 1201 50-51 -8.3125 1201 52-53 -8.28125 1201 54-55 -7.5625 1201 56-57 -7.875 1201 58-59 -7.4375 1201 60-61 -8.0625 1201 62-63 -7.25 1201 64-65 -7.5 1201 66-67 -7.5 1201 68-69 -5.0 1201 70-71 -5.0 1201 72-73 -3.75 1201 74-75 -2.5 1201 76-77 -2.5 1201 78-79 -2.5 1201 80-81 -2.5 1202 1 1.1636655011655037 1202 2 -2.35606511544011 1202 3 1.1202752109002105 1202 4 -2.0585251554001545 1202 5 -2.7545454545454504 1202 6 -2.6553925241425205 1202 7 0.5801767676767753 1202 8 1.349522005772009 1202 9 -2.9776920995670935 1202 10-11 -1.2860524891774858 1202 12-13 0.9539214257964304 1202 14-15 -2.248626893939395 1202 16-17 -1.158167873792884 1202 18-19 0.8942155067155042 1202 20-21 1.0453454531579567 1202 22-23 1.2154990842490818 1202 24-25 -0.9026404151404108 1202 26-27 -1.8585164835164747 1202 28-29 -1.7201617826617905 1202 30-31 0.9962958916083977 1202 32-33 1.5611855158730137 1202 34-35 3.1510416666666643 1202 36-37 11.75 1202 38-39 -22.979166666666668 1202 40-41 -21.270833333333336 1202 42-43 -19.0625 1202 44-45 -16.9375 1202 46-47 -9.4375 1202 48-49 -8.5 1202 50-51 -8.3125 1202 52-53 -8.28125 1202 54-55 -7.5625 1202 56-57 -7.875 1202 58-59 -7.4375 1202 60-61 -8.0625 1202 62-63 -7.25 1202 64-65 -7.5 1202 66-67 -7.5 1202 68-69 -5.0 1202 70-71 -5.0 1202 72-73 -3.75 1202 74-75 -2.5 1202 76-77 -2.5 1202 78-79 -2.5 1202 80-81 -2.5 1203 1 -2.8363344988344963 1203 2 -2.35606511544011 1203 3 1.1202752109002105 1203 4 -6.0585251554001545 1203 5 -6.75454545454545 1203 6 -5.6553925241425205 1203 7 -7.419823232323225 1203 8 -5.650477994227991 1203 9 1.0223079004329065 1203 10-11 -6.786052489177486 1203 12-13 0.9539214257964304 1203 14-15 -2.748626893939395 1203 16-17 0.841832126207116 1203 18-19 -1.1057844932844958 1203 20-21 -2.9546545468420433 1203 22-23 -0.7845009157509182 1203 24-25 1.0973595848595892 1203 26-27 2.1414835164835253 1203 28-29 1.7798382173382095 1203 30-31 0.9962958916083977 1203 32-33 1.5611855158730137 1203 34-35 3.1510416666666643 1203 36-37 11.75 1203 38-39 17.020833333333332 1203 40-41 18.729166666666664 1203 42-43 20.9375 1203 44-45 -16.9375 1203 46-47 -9.4375 1203 48-49 -8.5 1203 50-51 -8.3125 1203 52-53 -8.28125 1203 54-55 -7.5625 1203 56-57 -7.875 1203 58-59 -7.4375 1203 60-61 -8.0625 1203 62-63 -7.25 1203 64-65 -7.5 1203 66-67 -7.5 1203 68-69 -5.0 1203 70-71 -5.0 1203 72-73 -3.75 1203 74-75 -2.5 1203 76-77 -2.5 1203 78-79 -2.5 1203 80-81 -2.5 1204 1 1.1636655011655037 1204 2 1.64393488455989 1204 3 1.1202752109002105 1204 4 1.9414748445998455 1204 5 1.2454545454545496 1204 6 1.3446074758574795 1204 7 0.5801767676767753 1204 8 1.349522005772009 1204 9 1.0223079004329065 1204 10-11 0.7139475108225142 1204 12-13 0.9539214257964304 1204 14-15 1.2513731060606048 1204 16-17 0.841832126207116 1204 18-19 -1.1057844932844958 1204 20-21 -0.9546545468420433 1204 22-23 1.2154990842490818 1204 24-25 1.0973595848595892 1204 26-27 2.1414835164835253 1204 28-29 1.7798382173382095 1204 30-31 0.9962958916083977 1204 32-33 1.5611855158730137 1204 34-35 3.1510416666666643 1204 36-37 -28.25 1204 38-39 -22.979166666666668 1204 40-41 -21.270833333333336 1204 42-43 -19.0625 1204 44-45 -16.9375 1204 46-47 -9.4375 1204 48-49 -8.5 1204 50-51 -8.3125 1204 52-53 -8.28125 1204 54-55 -7.5625 1204 56-57 -7.875 1204 58-59 -7.4375 1204 60-61 -8.0625 1204 62-63 -7.25 1204 64-65 -7.5 1204 66-67 -7.5 1204 68-69 -5.0 1204 70-71 -5.0 1204 72-73 -3.75 1204 74-75 -2.5 1204 76-77 -2.5 1204 78-79 -2.5 1204 80-81 -2.5 1301 1 -2.036334498834499 1301 2 1.64393488455989 1301 3 1.1202752109002105 1301 4 1.9414748445998455 1301 5 1.2454545454545496 1301 6 1.3446074758574795 1301 7 0.5801767676767753 1301 8 1.349522005772009 1301 9 1.0223079004329065 1301 10-11 -0.8860524891774872 1301 12-13 -0.6460785742035711 1301 14-15 1.2513731060606048 1301 16-17 0.841832126207116 1301 18-19 -0.7057844932844972 1301 20-21 1.0453454531579567 1301 22-23 1.2154990842490818 1301 24-25 -1.702640415140408 1301 26-27 -0.6585164835164719 1301 28-29 1.7798382173382095 1301 30-31 -5.003704108391602 1301 32-33 1.5611855158730137 1301 34-35 -36.848958333333336 1301 36-37 -28.25 1301 38-39 -22.979166666666668 1301 40-41 -21.270833333333336 1301 42-43 -19.0625 1301 44-45 -16.9375 1301 46-47 -9.4375 1301 48-49 -8.5 1301 50-51 -8.3125 1301 52-53 -8.28125 1301 54-55 -7.5625 1301 56-57 -7.875 1301 58-59 -7.4375 1301 60-61 -8.0625 1301 62-63 -7.25 1301 64-65 -7.5 1301 66-67 -7.5 1301 68-69 -5.0 1301 70-71 -5.0 1301 72-73 -3.75 1301 74-75 -2.5 1301 76-77 -2.5 1301 78-79 -2.5 1301 80-81 -2.5 1302 1 1.1636655011655037 1302 2 -7.689398448773442 1302 3 -4.213058122433125 1302 4 -3.39185848873349 1302 5 1.2454545454545496 1302 6 1.3446074758574795 1302 7 0.5801767676767753 1302 8 1.349522005772009 1302 9 -4.311025432900429 1302 10-11 0.7139475108225142 1302 12-13 -1.712745240870241 1302 14-15 1.2513731060606048 1302 16-17 0.841832126207116 1302 18-19 0.8942155067155042 1302 20-21 1.0453454531579567 1302 22-23 1.2154990842490818 1302 24-25 -1.5693070818070822 1302 26-27 -7.858516483516475 1302 28-29 -0.8868284493284619 1302 30-31 -1.6703707750582737 1302 32-33 -13.772147817460322 1302 34-35 3.1510416666666643 1302 36-37 -28.25 1302 38-39 -22.979166666666668 1302 40-41 -21.270833333333336 1302 42-43 -19.0625 1302 44-45 -16.9375 1302 46-47 -9.4375 1302 48-49 -8.5 1302 50-51 -8.3125 1302 52-53 -8.28125 1302 54-55 -7.5625 1302 56-57 -7.875 1302 58-59 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52-53 -8.28125 1303 54-55 -7.5625 1303 56-57 -7.875 1303 58-59 -7.4375 1303 60-61 -8.0625 1303 62-63 -7.25 1303 64-65 -7.5 1303 66-67 -7.5 1303 68-69 -5.0 1303 70-71 -5.0 1303 72-73 -3.75 1303 74-75 -2.5 1303 76-77 -2.5 1303 78-79 -2.5 1303 80-81 -2.5 1304 1 1.1636655011655037 1304 2 1.64393488455989 1304 3 1.1202752109002105 1304 4 1.9414748445998455 1304 5 -4.354545454545452 1304 6 -4.255392524142522 1304 7 0.5801767676767753 1304 8 -1.8504779942279939 1304 9 -2.1776920995670963 1304 10-11 0.7139475108225142 1304 12-13 -3.4460785742035682 1304 14-15 1.2513731060606048 1304 16-17 -0.7581678737928854 1304 18-19 -0.7057844932844972 1304 20-21 -0.5546545468420447 1304 22-23 1.2154990842490818 1304 24-25 -0.5026404151404122 1304 26-27 -0.6585164835164719 1304 28-29 1.7798382173382095 1304 30-31 -1.8037041083915994 1304 32-33 -0.4388144841269863 1304 34-35 3.1510416666666643 1304 36-37 -28.25 1304 38-39 -22.979166666666668 1304 40-41 -21.270833333333336 1304 42-43 -19.0625 1304 44-45 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28.4375 2303 56-57 32.125 2303 58-59 18.5625 2303 60-61 23.9375 2303 62-63 32.75 2303 64-65 32.5 2303 66-67 32.5 2303 68-69 -5.0 2303 70-71 -5.0 2303 72-73 -3.75 2303 74-75 -2.5 2303 76-77 -2.5 2303 78-79 -2.5 2303 80-81 -2.5 2304 1 -0.8363344988344963 2304 2 -2.35606511544011 2304 3 -4.3797247890997895 2304 4 -5.5585251554001545 2304 5 -0.7545454545454504 2304 6 -6.6553925241425205 2304 7 -1.4198232323232247 2304 8 -0.650477994227991 2304 9 -4.4776920995670935 2304 10-11 0.7139475108225142 2304 12-13 -1.7960785742035696 2304 14-15 -0.4986268939393952 2304 16-17 -1.908167873792884 2304 18-19 -0.1057844932844958 2304 20-21 0.04534545315795668 2304 22-23 0.21549908424908182 2304 24-25 0.09735958485958918 2304 26-27 0.1414835164835253 2304 28-29 -0.9701617826617905 2304 30-31 -0.7537041083916023 2304 32-33 -0.18881448412698632 2304 34-35 3.1510416666666643 2304 36-37 11.75 2304 38-39 17.020833333333332 2304 40-41 18.729166666666664 2304 42-43 20.9375 2304 44-45 19.0625 2304 46-47 -9.4375 2304 48-49 -8.5 2304 50-51 -8.3125 2304 52-53 -8.28125 2304 54-55 -7.5625 2304 56-57 -7.875 2304 58-59 -7.4375 2304 60-61 -8.0625 2304 62-63 -7.25 2304 64-65 -7.5 2304 66-67 -7.5 2304 68-69 -5.0 2304 70-71 -5.0 2304 72-73 -3.75 2304 74-75 -2.5 2304 76-77 -2.5 2304 78-79 -2.5 2304 80-81 -2.5 2401 1 1.1636655011655037 2401 2 1.64393488455989 2401 3 1.1202752109002105 2401 4 -1.1696362665112687 2401 5 1.2454545454545496 2401 6 1.3446074758574795 2401 7 -1.1976010101010033 2401 8 1.349522005772009 2401 9 1.0223079004329065 2401 10-11 0.7139475108225142 2401 12-13 0.9539214257964304 2401 14-15 1.2513731060606048 2401 16-17 0.841832126207116 2401 18-19 0.005326617826618474 2401 20-21 -1.3990989912864862 2401 22-23 -0.5622786935286967 2401 24-25 -0.6804181929181894 2401 26-27 2.1414835164835253 2401 28-29 1.7798382173382095 2401 30-31 0.9962958916083977 2401 32-33 0.4183283730158749 2401 34-35 3.1510416666666643 2401 36-37 11.75 2401 38-39 17.020833333333332 2401 40-41 18.729166666666664 2401 42-43 20.9375 2401 44-45 23.0625 2401 46-47 30.5625 2401 48-49 31.5 2401 50-51 31.6875 2401 52-53 31.71875 2401 54-55 32.4375 2401 56-57 32.125 2401 58-59 32.5625 2401 60-61 31.9375 2401 62-63 -7.25 2401 64-65 -7.5 2401 66-67 -7.5 2401 68-69 -5.0 2401 70-71 -5.0 2401 72-73 -3.75 2401 74-75 -2.5 2401 76-77 -2.5 2401 78-79 -2.5 2401 80-81 -2.5 2402 1 -0.4363344988344977 2402 2 -1.5560651154401128 2402 3 -0.4797247890997909 2402 4 0.3414748445998441 2402 5 1.2454545454545496 2402 6 1.3446074758574795 2402 7 0.5801767676767753 2402 8 1.349522005772009 2402 9 1.0223079004329065 2402 10-11 0.7139475108225142 2402 12-13 0.9539214257964304 2402 14-15 1.2513731060606048 2402 16-17 0.841832126207116 2402 18-19 0.09421550671550705 2402 20-21 1.0453454531579567 2402 22-23 -1.7845009157509182 2402 24-25 1.0973595848595892 2402 26-27 0.7414835164835267 2402 28-29 -2.0201617826617877 2402 30-31 0.9962958916083977 2402 32-33 0.5611855158730137 2402 34-35 3.1510416666666643 2402 36-37 11.75 2402 38-39 17.020833333333332 2402 40-41 18.729166666666664 2402 42-43 -19.0625 2402 44-45 -16.9375 2402 46-47 -9.4375 2402 48-49 -8.5 2402 50-51 -8.3125 2402 52-53 -8.28125 2402 54-55 -7.5625 2402 56-57 -7.875 2402 58-59 -7.4375 2402 60-61 -8.0625 2402 62-63 -7.25 2402 64-65 -7.5 2402 66-67 -7.5 2402 68-69 -5.0 2402 70-71 -5.0 2402 72-73 -3.75 2402 74-75 -2.5 2402 76-77 -2.5 2402 78-79 -2.5 2402 80-81 -2.5 2403 1 -1.7454254079254028 2403 2 -1.2651560245310165 2403 3 -0.3342702436452427 2403 4 0.4869293900543923 2403 5 -0.20909090909090366 2403 6 -0.10993797868797373 2403 7 -0.874368686868678 2403 8 -0.10502344877344427 2403 9 -1.886783008658 2403 10-11 -0.013325216450212451 2403 12-13 -0.5006240287490229 2403 14-15 0.5241003787878782 2403 16-17 0.11455939893438938 2403 18-19 0.8942155067155042 2403 20-21 -0.2273818195693167 2403 22-23 1.2154990842490818 2403 24-25 -0.9026404151404108 2403 26-27 2.1414835164835253 2403 28-29 -0.22016178266179054 2403 30-31 -0.45824956293705554 2403 32-33 0.4183283730158749 2403 34-35 3.1510416666666643 2403 36-37 11.75 2403 38-39 17.020833333333332 2403 40-41 14.729166666666664 2403 42-43 20.9375 2403 44-45 23.0625 2403 46-47 30.5625 2403 48-49 31.5 2403 50-51 31.6875 2403 52-53 23.71875 2403 54-55 32.4375 2403 56-57 32.125 2403 58-59 32.5625 2403 60-61 31.9375 2403 62-63 24.75 2403 64-65 32.5 2403 66-67 32.5 2403 68-69 35.0 2403 70-71 35.0 2403 72-73 36.25 2403 74-75 37.5 2403 76-77 37.5 2403 78-79 37.5 2403 80-81 37.5 2404 1 -1.2978729603729562 2404 2 1.64393488455989 2404 3 -0.11049401986902296 2404 4 -0.5200636169386144 2404 5 1.2454545454545496 2404 6 -0.8092386779886738 2404 7 0.5801767676767753 2404 8 1.349522005772009 2404 9 1.0223079004329065 2404 10-11 0.7139475108225142 2404 12-13 0.33853681041181716 2404 14-15 1.2513731060606048 2404 16-17 -0.8504755661005774 2404 18-19 -0.3365537240537222 2404 20-21 -1.26234685453435 2404 22-23 -1.8614239926739984 2404 24-25 -1.3641788766788778 2404 26-27 -1.2431318681318615 2404 28-29 -1.1432387057387103 2404 30-31 -0.08062718531468249 2404 32-33 0.672296626984128 2404 34-35 3.1510416666666643 2404 36-37 11.75 2404 38-39 17.020833333333332 2404 40-41 18.729166666666664 2404 42-43 20.9375 2404 44-45 23.0625 2404 46-47 30.5625 2404 48-49 31.5 2404 50-51 31.6875 2404 52-53 31.71875 2404 54-55 28.4375 2404 56-57 32.125 2404 58-59 32.5625 2404 60-61 31.9375 2404 62-63 32.75 2404 64-65 32.5 2404 66-67 32.5 2404 68-69 35.0 2404 70-71 35.0 2404 72-73 -3.75 2404 74-75 -2.5 2404 76-77 -2.5 2404 78-79 -2.5 2404 80-81 -2.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 31 4.0 32 28.0 33 30.0 34 62.0 35 55.0 36 97.0 37 133.0 38 240.0 39 453.0 40 798.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.105263157894736 26.0 47.05263157894737 13.842105263157894 2 17.377567140600316 23.012111637704056 39.44181147972618 20.168509741969455 3 18.684210526315788 20.105263157894736 40.526315789473685 20.684210526315788 4 17.42105263157895 22.526315789473685 34.0 26.052631578947366 5 20.36842105263158 20.94736842105263 36.63157894736842 22.05263157894737 6 19.526315789473685 22.94736842105263 33.631578947368425 23.894736842105264 7 20.157894736842106 27.52631578947368 33.473684210526315 18.842105263157894 8 12.68421052631579 65.94736842105263 7.894736842105263 13.473684210526315 9 60.68421052631579 17.157894736842106 14.421052631578949 7.7368421052631575 10-11 11.552631578947368 34.78947368421053 42.0 11.657894736842104 12-13 43.684210526315795 8.605263157894736 11.473684210526315 36.23684210526316 14-15 39.78947368421053 40.86842105263158 8.342105263157896 11.0 16-17 34.5 41.973684210526315 8.263157894736842 15.263157894736842 18-19 37.0 25.57894736842105 13.921052631578949 23.5 20-21 35.3421052631579 5.7368421052631575 26.894736842105267 32.026315789473685 22-23 32.39473684210527 7.973684210526316 32.44736842105263 27.184210526315788 24-25 37.78947368421053 13.078947368421053 39.10526315789474 10.026315789473685 26-27 40.131578947368425 29.789473684210527 24.81578947368421 5.263157894736842 28-29 49.13157894736842 32.21052631578947 13.421052631578947 5.2368421052631575 30-31 32.113714135298764 51.69781521453014 11.10818636483285 5.080284285338247 32-33 33.34644121116791 43.96382225717657 17.18442784113252 5.505308690523004 34-35 27.123483226266952 31.834403997144893 31.977159172019988 9.064953604568165 36-37 30.719794344473005 37.40359897172236 20.69408740359897 11.182519280205655 38-39 34.98349834983499 37.95379537953795 13.861386138613863 13.2013201320132 40-41 34.172661870503596 37.589928057553955 16.546762589928058 11.690647482014388 42-43 33.13609467455622 38.06706114398422 18.54043392504931 10.256410256410255 44-45 19.25133689839572 31.016042780748666 32.62032085561498 17.11229946524064 46-47 29.00763358778626 29.389312977099237 21.755725190839694 19.84732824427481 48-49 37.295081967213115 32.37704918032787 17.21311475409836 13.114754098360656 50-51 30.869565217391305 30.0 17.82608695652174 21.304347826086957 52-53 32.6829268292683 27.80487804878049 16.585365853658537 22.926829268292686 54-55 29.78723404255319 23.404255319148938 27.127659574468083 19.680851063829788 56-57 32.57142857142858 26.857142857142858 25.142857142857146 15.428571428571427 58-59 49.693251533742334 16.56441717791411 18.404907975460123 15.337423312883436 60-61 38.19444444444444 35.41666666666667 19.444444444444446 6.944444444444445 62-63 43.11926605504588 31.19266055045872 17.431192660550458 8.256880733944955 64-65 35.714285714285715 26.190476190476193 14.285714285714285 23.809523809523807 66-67 28.985507246376812 37.68115942028986 23.18840579710145 10.144927536231885 68-69 17.857142857142858 28.57142857142857 25.0 28.57142857142857 70-71 27.27272727272727 31.818181818181817 9.090909090909092 31.818181818181817 72-73 24.324324324324326 21.62162162162162 32.432432432432435 21.62162162162162 74-75 30.0 20.0 16.666666666666664 33.33333333333333 76-77 21.73913043478261 30.434782608695656 26.08695652173913 21.73913043478261 78-79 28.57142857142857 19.047619047619047 33.33333333333333 19.047619047619047 80-81 23.52941176470588 17.647058823529413 29.411764705882355 29.411764705882355 82-83 30.0 10.0 30.0 30.0 84-85 16.666666666666664 16.666666666666664 33.33333333333333 33.33333333333333 86-87 50.0 0.0 25.0 25.0 88-89 0.0 66.66666666666666 0.0 33.33333333333333 90 0.0 100.0 0.0 0.0 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 2.5 17 2.5 18 1.0 19 0.5 20 2.0 21 3.0 22 3.0 23 3.0 24 2.5 25 2.5 26 2.5 27 2.0 28 1.5 29 0.5 30 1.0 31 1.5 32 1.0 33 1.0 34 3.0 35 5.0 36 8.5 37 20.5 38 35.0 39 59.5 40 101.0 41 140.5 42 195.0 43 272.0 44 368.5 45 432.5 46 455.5 47 499.0 48 500.5 49 446.0 50 376.5 51 323.5 52 286.0 53 211.0 54 150.0 55 137.0 56 104.5 57 69.0 58 55.0 59 50.5 60 42.0 61 23.0 62 14.5 63 10.5 64 7.5 65 6.5 66 7.5 67 8.0 68 7.0 69 5.0 70 3.5 71 2.5 72 1.5 73 1.5 74 1.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05263157894736842 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 453.0 32-33 639.0 34-35 376.0 36-37 120.0 38-39 28.0 40-41 25.0 42-43 46.0 44-45 80.0 46-47 9.0 48-49 5.0 50-51 14.0 52-53 9.0 54-55 7.0 56-57 4.0 58-59 9.0 60-61 19.0 62-63 13.0 64-65 8.0 66-67 7.0 68-69 6.0 70-71 4.0 72-73 3.0 74-75 4.0 76-77 1.0 78-79 2.0 80-81 4.0 82-83 2.0 84-85 1.0 86-87 0.0 88-89 1.0 90-91 1.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.10526315789473684 0.0 0.0 0.0 0.0 2 0.15789473684210525 0.0 0.0 0.0 0.0 3 0.15789473684210525 0.0 0.0 0.0 0.0 4 0.15789473684210525 0.0 0.0 0.0 0.0 5 0.3157894736842105 0.0 0.0 0.0 0.0 6 0.6842105263157895 0.0 0.0 0.0 0.0 7 2.8947368421052633 0.0 0.0 0.0 0.0 8 28.0 0.0 0.0 0.0 0.0 9 29.894736842105264 0.0 0.0 0.0 0.0 10-11 29.921052631578945 0.0 0.0 0.0 0.0 12-13 29.94736842105263 0.0 0.0 0.0 0.0 14-15 29.94736842105263 0.0 0.0 0.0 0.0 16-17 29.94736842105263 0.0 0.0 0.0 0.0 18-19 29.94736842105263 0.0 0.0 0.0 0.0 20-21 30.10526315789474 0.0 0.0 0.0 0.0 22-23 30.210526315789473 0.0 0.0 0.0 0.0 24-25 30.36842105263158 0.0 0.0 0.0 0.0 26-27 30.526315789473685 0.0 0.0 0.0 0.0 28-29 30.789473684210527 0.0 0.0 0.0 0.0 30-31 30.894736842105264 0.0 0.0 0.0 0.0 32-33 30.921052631578945 0.0 0.0 0.0 0.0 34-35 31.05263157894737 0.0 0.0 0.0 0.0 36-37 31.473684210526315 0.0 0.0 0.0 0.0 38-39 31.973684210526315 0.0 0.0 0.0 0.0 40-41 32.5 0.0 0.0 0.0 0.0 42-43 32.84210526315789 0.0 0.0 0.0 0.0 44-45 33.0 0.0 0.0 0.0 0.0 46-47 33.0 0.0 0.0 0.0 0.0 48-49 33.0 0.0 0.0 0.0 0.0 50-51 33.0 0.0 0.0 0.0 0.0 52-53 33.0 0.0 0.0 0.0 0.0 54-55 33.0 0.0 0.0 0.0 0.0 56-57 33.0 0.0 0.0 0.0 0.0 58-59 33.0 0.0 0.0 0.0 0.0 60-61 33.0 0.0 0.0 0.0 0.0 62-63 33.0 0.0 0.0 0.0 0.0 64-65 33.0 0.0 0.0 0.0 0.0 66-67 33.0 0.0 0.0 0.0 0.0 68-69 33.0 0.0 0.0 0.0 0.0 70-71 33.0 0.0 0.0 0.0 0.0 72-73 33.0 0.0 0.0 0.0 0.0 74-75 33.0 0.0 0.0 0.0 0.0 76-77 33.0 0.0 0.0 0.0 0.0 78-79 33.0 0.0 0.0 0.0 0.0 >>END_MODULE