##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-EC4_S108_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93877
Sequences flagged as poor quality	0
Sequence length	61
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.402068664316076	40.0	40.0	40.0	40.0	40.0
2	39.43935149184571	40.0	40.0	40.0	40.0	40.0
3	39.28274231174835	40.0	40.0	40.0	40.0	40.0
4	39.496063998636515	40.0	40.0	40.0	40.0	40.0
5	39.26001043919171	40.0	40.0	40.0	40.0	40.0
6	39.30104285394719	40.0	40.0	40.0	40.0	40.0
7	39.23631986535573	40.0	40.0	40.0	40.0	40.0
8	38.8816856098938	40.0	40.0	40.0	40.0	40.0
9	39.23056765767973	40.0	40.0	40.0	40.0	40.0
10	39.376034598463946	40.0	40.0	40.0	40.0	40.0
11	39.36444496522045	40.0	40.0	40.0	40.0	40.0
12	39.36832237928353	40.0	40.0	40.0	40.0	40.0
13	38.06014252692353	40.0	40.0	40.0	24.0	40.0
14	39.35669013709428	40.0	40.0	40.0	40.0	40.0
15	39.40083300488938	40.0	40.0	40.0	40.0	40.0
16	39.182760420550295	40.0	40.0	40.0	40.0	40.0
17	39.3123768335162	40.0	40.0	40.0	40.0	40.0
18	39.2891123491377	40.0	40.0	40.0	40.0	40.0
19	39.29171149482834	40.0	40.0	40.0	40.0	40.0
20	26.2958126058566	24.0	12.0	40.0	12.0	40.0
21	27.079646771839748	24.0	12.0	40.0	12.0	40.0
22	27.771871704464353	24.0	12.0	40.0	12.0	40.0
23	36.382841377547216	40.0	40.0	40.0	24.0	40.0
24	38.18788414627651	40.0	40.0	40.0	40.0	40.0
25	27.861478317372733	24.0	12.0	40.0	12.0	40.0
26	37.87159794198792	40.0	40.0	40.0	24.0	40.0
27	38.330112807183866	40.0	40.0	40.0	40.0	40.0
28	25.067098437316915	24.0	12.0	40.0	12.0	40.0
29	35.38089201827924	40.0	40.0	40.0	24.0	40.0
30	23.307988112104137	12.0	12.0	40.0	12.0	40.0
31	25.312994663229546	24.0	12.0	40.0	12.0	40.0
32	26.50621557996101	24.0	12.0	40.0	12.0	40.0
33	32.04746636556345	40.0	24.0	40.0	24.0	40.0
34	36.63777069995846	40.0	40.0	40.0	24.0	40.0
35	22.63527807663219	12.0	12.0	40.0	12.0	40.0
36	30.24229576999691	40.0	24.0	40.0	12.0	40.0
37	33.753400726482525	40.0	24.0	40.0	24.0	40.0
38	23.588568019855767	24.0	12.0	40.0	12.0	40.0
39	32.89768526902223	40.0	24.0	40.0	24.0	40.0
40	21.12969097862096	12.0	12.0	40.0	12.0	40.0
41	23.773192581782546	24.0	12.0	40.0	12.0	40.0
42	21.06462711846352	12.0	12.0	40.0	12.0	40.0
43	22.562203734674096	24.0	12.0	40.0	12.0	40.0
44	21.030497352919244	12.0	12.0	40.0	12.0	40.0
45	26.945854682190525	24.0	24.0	40.0	12.0	40.0
46	21.17604951159496	12.0	12.0	40.0	12.0	40.0
47	20.043248079934383	12.0	12.0	24.0	12.0	40.0
48	25.787445274135305	24.0	24.0	40.0	12.0	40.0
49	20.19597984596866	12.0	12.0	24.0	12.0	40.0
50	25.543594277618585	24.0	24.0	24.0	12.0	40.0
51	19.86731574294023	12.0	12.0	24.0	12.0	40.0
52	25.813308904204437	24.0	24.0	24.0	12.0	40.0
53	19.189450025032755	12.0	12.0	24.0	12.0	40.0
54	20.065191686994684	12.0	12.0	24.0	12.0	40.0
55	23.876050576818603	24.0	12.0	24.0	12.0	40.0
56	19.049969641126154	12.0	12.0	24.0	12.0	40.0
57	23.161093771637354	24.0	12.0	24.0	12.0	40.0
58	23.40701130202286	24.0	12.0	24.0	12.0	40.0
59	20.540963175218636	12.0	12.0	24.0	12.0	40.0
60	18.242189247632542	12.0	12.0	24.0	12.0	40.0
61	21.851635650904907	24.0	12.0	24.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.28679758720164017
1101	2	0.05252366864372249
1101	3	0.3236561650098224
1101	4	0.29221134720886255
1101	5	0.004486178894623549
1101	6	0.14272711541850214
1101	7	-0.05377550741847159
1101	8	0.5439688629902832
1101	9	0.4108595320146833
1101	10	-0.018312040796942597
1101	11	-0.07930288306813793
1101	12	0.07908079530959355
1101	13	0.49511674043720433
1101	14	0.20518150034135374
1101	15	-0.053135805807883685
1101	16	0.05181667804946244
1101	17	0.29068252999206123
1101	18	0.06348241721379821
1101	19	-0.027548570597311084
1101	20	-1.0003200516768729
1101	21	-0.8287112270082027
1101	22	-0.11469588811628384
1101	23	0.5190428625991359
1101	24	-0.08751739491989952
1101	25	-0.7438928429794345
1101	26	0.4804612544313116
1101	27	-0.0688443785398789
1101	28	-0.29295426279420766
1101	29	0.6707983654171059
1101	30	-0.261465497517424
1101	31	0.1343151763366066
1101	32	0.9118633768882844
1101	33	-0.11541097015083324
1101	34	0.3851091557835886
1101	35	0.041574903352984194
1101	36	-0.5972616599496732
1101	37	0.5808538261140725
1101	38	0.06478604603490012
1101	39	0.5387784281222352
1101	40	0.22934842649636167
1101	41	1.0984752122424304
1101	42	0.3367667003982824
1101	43	0.079922264075595
1101	44	0.5721578067380797
1101	45	0.28709986051055836
1101	46	-0.008802268792404533
1101	47	0.5811401029698153
1101	48	-0.08835640023514202
1101	49	0.8251804287197935
1101	50	0.5037540247110854
1101	51	0.5266402358290279
1101	52	0.07187340741841197
1101	53	-0.12966990197238104
1101	54	1.2727806757055262
1101	55	0.4338190364163381
1101	56	0.7237813269861206
1101	57	0.4023359824042245
1101	58	0.056485814256966194
1101	59	0.011814722520899323
1101	60	0.3057751773105579
1101	61	-0.4802151156118626
1102	1	0.015139502507672375
1102	2	-0.15287286606272943
1102	3	-0.019686166511981185
1102	4	0.07858563220963788
1102	5	-0.053879163413114384
1102	6	-0.04958835234855741
1102	7	-0.09877437768996344
1102	8	0.48902838184486086
1102	9	0.1511408938726717
1102	10	0.2746087236130421
1102	11	0.1982535736275821
1102	12	-0.19799998622470127
1102	13	-0.2749537784054823
1102	14	0.18239666010874345
1102	15	0.04494841247944947
1102	16	-0.019915136294521574
1102	17	-0.1362834949095344
1102	18	-0.033555315718380996
1102	19	-0.4198378762304955
1102	20	-1.2624646331421907
1102	21	-1.2674264283971723
1102	22	-0.16250124184022496
1102	23	-1.1206569863740015
1102	24	0.16463800815533602
1102	25	1.1361656650654197
1102	26	-0.2799530586342911
1102	27	-0.2996419668668082
1102	28	-0.3156439679945251
1102	29	-0.5275225012630358
1102	30	-0.029431153770769214
1102	31	-0.49134036349524024
1102	32	-0.5882733797206292
1102	33	-0.05029104055075706
1102	34	0.07287597981945737
1102	35	-0.6801194515720397
1102	36	-0.9552547864435894
1102	37	-0.23901988213056313
1102	38	0.043142826189733086
1102	39	-0.22682074420298193
1102	40	0.14781770382687043
1102	41	0.018264488145913305
1102	42	-0.16202627387958302
1102	43	-0.3673075227030118
1102	44	-0.20423919175165395
1102	45	-0.6208688876313353
1102	46	-0.40351751774889166
1102	47	-0.91095774341089
1102	48	0.09977312611131595
1102	49	-0.5883834485084449
1102	50	-0.5559075324115526
1102	51	-0.20233194906590768
1102	52	0.08367015142193424
1102	53	-0.20282874180066202
1102	54	0.25573961304721493
1102	55	0.2153890022866456
1102	56	-0.3269375371015961
1102	57	-0.6944758048262756
1102	58	-0.3320932251170454
1102	59	-0.5607079017789651
1102	60	-0.07233900851194264
1102	61	-1.0085950849308176
1103	1	-0.26831598229347975
1103	2	-0.17325013387481647
1103	3	-0.3014115059328617
1103	4	-0.22039310142006485
1103	5	-0.36232563704041354
1103	6	-0.04209105570071614
1103	7	-0.08983529322522799
1103	8	-0.04183789233512414
1103	9	0.10303324038233797
1103	10	-0.09405642116648494
1103	11	-0.26609822259973726
1103	12	0.0868011672055573
1103	13	-0.8583491887142642
1103	14	0.14563107077797355
1103	15	-0.04598033917260125
1103	16	-0.02505751284144253
1103	17	-0.05852307198510687
1103	18	-0.05350004718540191
1103	19	-0.15959517528131784
1103	20	0.02168771577046158
1103	21	0.5059245267872541
1103	22	0.6133728420237681
1103	23	-0.14749826956516188
1103	24	0.3200146752225024
1103	25	0.5174464532427585
1103	26	-0.7096058280683906
1103	27	0.051433366546625336
1103	28	-0.13176796174677818
1103	29	-0.3359569599918615
1103	30	1.0395583932675585
1103	31	1.1281718316267089
1103	32	0.5626577749111128
1103	33	0.8064673398409248
1103	34	0.45129722215995827
1103	35	1.0361847656573246
1103	36	-1.3950961897837324
1103	37	0.36826114634474294
1103	38	0.1575246596632418
1103	39	0.19442159629924305
1103	40	1.6234875344168032
1103	41	-0.2843187150260178
1103	42	0.9865019010573377
1103	43	1.2793102833765246
1103	44	0.2017682574854014
1103	45	0.8033291178955189
1103	46	1.009674840775677
1103	47	0.6841882376055679
1103	48	-0.1400923070257747
1103	49	1.4153651998154757
1103	50	0.3449214955831579
1103	51	0.8900626201999806
1103	52	-0.44642716925570625
1103	53	0.7444739134918983
1103	54	1.4936586326314973
1103	55	-0.4053006528857672
1103	56	0.8590290614806477
1103	57	-0.09901743644948624
1103	58	-0.4599317448815512
1103	59	0.3078250791173467
1103	60	0.8566048819121832
1103	61	0.34798307198375866
1104	1	-0.100789149683294
1104	2	0.19962038450837838
1104	3	0.19057296540762536
1104	4	0.07355528862332505
1104	5	0.18702352943595457
1104	6	-0.07981656380759006
1104	7	0.06335036560098928
1104	8	0.5229485885965914
1104	9	-0.026859429176916194
1104	10	0.06744163775108092
1104	11	-0.2564156466691614
1104	12	0.3084956179934082
1104	13	-0.5727131987622158
1104	14	-0.1265771959495865
1104	15	0.24413155852094093
1104	16	0.10109000942391333
1104	17	-0.2794867623253694
1104	18	-0.11607141051035796
1104	19	0.41907575415462617
1104	20	-0.47431593803177563
1104	21	-0.368887074034852
1104	22	0.41040412770543
1104	23	-0.8058220877884992
1104	24	0.0629696877824486
1104	25	-0.986823928122849
1104	26	0.5710518563344422
1104	27	0.19393165382682298
1104	28	-0.9692194346858045
1104	29	-0.3494760282722922
1104	30	-0.19487869282515646
1104	31	-0.4771261816183241
1104	32	0.4873894488092638
1104	33	-0.06916636648471552
1104	34	-0.26914123410859503
1104	35	-0.18793032911316843
1104	36	0.15146695248619224
1104	37	0.5707731353833339
1104	38	0.02279070213869261
1104	39	-0.37648500338104895
1104	40	-0.26370132148636927
1104	41	-0.017317116487539863
1104	42	-0.05154331474535567
1104	43	-0.090500175632382
1104	44	-0.46678620700420836
1104	45	-0.802699655797106
1104	46	-0.3997793725150274
1104	47	0.5756703111383388
1104	48	0.4447900862146916
1104	49	-0.30548430920485004
1104	50	-0.49563570925812783
1104	51	0.0359863470782642
1104	52	-0.26282360679852346
1104	53	-0.30352791340921925
1104	54	-0.60855648497526
1104	55	-0.19520702420341962
1104	56	-0.16915088827957092
1104	57	-0.6036988705668662
1104	58	0.2461655376030336
1104	59	-0.9336455762834319
1104	60	0.04345119385554952
1104	61	0.551134476413992
1201	1	0.04495111297244847
1201	2	0.015177686113496236
1201	3	0.08245757647508611
1201	4	0.049624432186014644
1201	5	-0.342687998232698
1201	6	0.17345540835539452
1201	7	0.2907608493814351
1201	8	0.548226397553826
1201	9	-0.304915327706496
1201	10	0.12172382430400575
1201	11	0.03407711346104492
1201	12	0.15085727474500743
1201	13	0.03072153016358925
1201	14	-0.03601708819338256
1201	15	0.3173159787701252
1201	16	0.07055908563897617
1201	17	0.30711106010139133
1201	18	0.13562911704604375
1201	19	-0.09016209521118412
1201	20	0.549098586606231
1201	21	1.1757082789789557
1201	22	0.47029861642470294
1201	23	0.8383579548649962
1201	24	0.12901525967603789
1201	25	-0.10160673202901194
1201	26	-0.348647324269443
1201	27	0.15967416140343005
1201	28	-0.35339274257670183
1201	29	-1.105565376122783
1201	30	0.6802363593692533
1201	31	0.8027481028131511
1201	32	-0.1700131777481495
1201	33	0.26573092838563994
1201	34	0.5948390105877763
1201	35	0.48352550206877964
1201	36	-0.472127165821135
1201	37	-0.4775600108732547
1201	38	-0.014070898489887185
1201	39	0.1633286681870274
1201	40	0.49081658175753873
1201	41	1.10527801203418
1201	42	0.2182961734126856
1201	43	0.9164815282625582
1201	44	1.3617916356268402
1201	45	0.604147352845839
1201	46	-0.02995110896131692
1201	47	1.1056961277283044
1201	48	0.7545891657971353
1201	49	0.9807655504875967
1201	50	0.3818589590548136
1201	51	0.4285781082186837
1201	52	0.3844319774421301
1201	53	0.5675013828080893
1201	54	1.1822617886805915
1201	55	0.28318561245613694
1201	56	0.5085907213286909
1201	57	1.3003981100145694
1201	58	-0.4141105876635507
1201	59	-0.23641805355360646
1201	60	1.2645534500010172
1201	61	0.012039179523210208
1202	1	-0.14157110730828748
1202	2	-0.10205686453010543
1202	3	0.265764699778714
1202	4	0.08452454285058764
1202	5	0.19148405605118768
1202	6	0.24903348512165735
1202	7	0.10554330613489071
1202	8	0.47092428327810154
1202	9	0.24300243104474362
1202	10	0.15587840523475194
1202	11	0.10816413075966835
1202	12	0.18501185567575362
1202	13	0.7459641185496295
1202	14	0.06481580830612188
1202	15	0.3273340345683664
1202	16	0.19776509647245888
1202	17	-0.028377320118771365
1202	18	0.12780105484090853
1202	19	0.3245854148359939
1202	20	0.7279325315069229
1202	21	-0.015322066410991653
1202	22	0.8042959371772227
1202	23	0.30530419518184715
1202	24	0.3940044844416022
1202	25	-0.07542000012849215
1202	26	-0.05360393210915504
1202	27	0.11186706722205741
1202	28	1.1291397287378757
1202	29	-0.19508718869194297
1202	30	1.8079432729926452
1202	31	0.22645361856828927
1202	32	1.1838688188736697
1202	33	0.1717475863156288
1202	34	-0.40482317125158573
1202	35	1.091831169259649
1202	36	-0.29799937736519766
1202	37	-0.889139019551692
1202	38	0.47462384203081953
1202	39	-0.6338755953111388
1202	40	1.1051796780357144
1202	41	0.4867679066116146
1202	42	0.2094430078235412
1202	43	0.7851285082846893
1202	44	0.7613606262494734
1202	45	-1.078979212475378
1202	46	0.20507713962625118
1202	47	0.0631193905857792
1202	48	-0.5305177128795506
1202	49	0.04889997881772601
1202	50	-0.15618053072327598
1202	51	0.22742486691167585
1202	52	-0.32745572714171445
1202	53	-0.08041055149210408
1202	54	0.37485308695072916
1202	55	-0.4306591076789914
1202	56	-0.19896359167730893
1202	57	0.3166716262356637
1202	58	-0.42864841747309157
1202	59	-0.30011425018707527
1202	60	0.07608586307108567
1202	61	-1.2404041776903725
1203	1	0.12321456822073884
1203	2	-0.10162278965218974
1203	3	0.3117766368378554
1203	4	0.22993962695259285
1203	5	0.1366459298077416
1203	6	0.08567663939910375
1203	7	0.02147747144507406
1203	8	0.6245867900605191
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2403	31	-1.4440135988368645
2403	32	-0.3338026041708666
2403	33	0.4076548255682617
2403	34	0.5652958519841178
2403	35	-0.2806455609012488
2403	36	1.5024603299696366
2403	37	1.060839360548897
2403	38	0.07577083458902223
2403	39	0.8288130098639854
2403	40	-0.25045628837378686
2403	41	-1.540495924434559
2403	42	-0.47538437434800684
2403	43	-1.2957981888774128
2403	44	-0.9965875315075188
2403	45	-0.7762095895719412
2403	46	-1.2474614917203262
2403	47	-0.642872735219278
2403	48	-0.9724284568316683
2403	49	-0.8750207330459077
2403	50	-0.005569484092564636
2403	51	-0.6527553747760422
2403	52	-0.19659844123561143
2403	53	-1.3366404961906753
2403	54	-1.1648478757037388
2403	55	-0.7514984149318984
2403	56	-1.3876939346371877
2403	57	-1.001049863944349
2403	58	-0.07171525709895477
2403	59	-0.07271842396555073
2403	60	-1.0823766207139869
2403	61	0.4981543439636624
2404	1	-0.14555736160382082
2404	2	-0.0026754212311388414
2404	3	-0.013135643863897428
2404	4	0.04397471467189007
2404	5	0.15629505261475884
2404	6	-0.07151634305704135
2404	7	0.08533712056787834
2404	8	0.21195521111314974
2404	9	0.10073438980762717
2404	10	-0.08302193840786032
2404	11	0.0842466049864683
2404	12	-0.08055515463352947
2404	13	0.7034898917455052
2404	14	-0.011610308532368663
2404	15	-0.03816424721857459
2404	16	0.09632179750338565
2404	17	-0.13944261221499232
2404	18	0.0651206424697719
2404	19	0.05695654885661128
2404	20	-0.7010709049191917
2404	21	-0.07016742723573444
2404	22	-0.34800646832105997
2404	23	0.04468563848082141
2404	24	-0.5356175086855401
2404	25	-0.9133581444583889
2404	26	0.49361256273620313
2404	27	-0.015693070234988227
2404	28	-0.780963364045629
2404	29	0.6304798216173353
2404	30	-1.2271082733991072
2404	31	-0.6851615017066237
2404	32	0.04173823468122961
2404	33	-0.216980936021141
2404	34	0.28026273940133706
2404	35	-0.8773784527334776
2404	36	0.6334095573427057
2404	37	0.47814620381601003
2404	38	0.04672006196209111
2404	39	1.27993883767855
2404	40	-0.5374320058130806
2404	41	-0.6047784851409617
2404	42	-0.9109693633104783
2404	43	-1.1942529350142799
2404	44	-0.13018576550310357
2404	45	-0.5617283533688493
2404	46	-0.8082561937070807
2404	47	-0.6601795784863853
2404	48	0.11278125619261559
2404	49	-0.9726807771960182
2404	50	0.0042318404107462015
2404	51	-0.4175235866965714
2404	52	-0.39907084741662757
2404	53	-0.49690539685292734
2404	54	-1.1187551604719488
2404	55	1.0420556689533136
2404	56	-0.15016634081820257
2404	57	-0.7735001950701772
2404	58	0.6835165309805156
2404	59	0.7574802515311383
2404	60	-0.30109626751310614
2404	61	1.060626750144678
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
15	36.0
16	85.0
17	161.0
18	176.0
19	271.0
20	375.0
21	477.0
22	732.0
23	1106.0
24	2232.0
25	5620.0
26	18116.0
27	20572.0
28	8131.0
29	2569.0
30	2461.0
31	3014.0
32	3156.0
33	2392.0
34	1953.0
35	2061.0
36	2263.0
37	1670.0
38	1884.0
39	6159.0
40	6205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.23595206391478	69.85352862849534	12.239680426098536	11.670838881491346
2	66.97061047967021	7.411826112892402	13.26309958775845	12.354463819678942
3	9.021475137415313	68.10047296433594	9.89070688993992	12.987345008308834
4	6.938866815087828	8.876508622985396	72.1944672283946	11.990157333532176
5	5.791620950818625	6.776952821244821	11.494828339209818	75.93659788872674
6	69.2342107225412	7.618479499770977	10.530800941657702	12.616508836030125
7	70.05123725726217	8.965987409056533	8.461071401940838	12.521703931740468
8	6.866431607315956	71.75559508718855	12.405594554576734	8.972378750918756
9	7.481065649733161	71.69594256314113	9.859710046124185	10.963281741001524
10	68.11253022572089	10.412561117206558	10.36782172417099	11.107086932901563
11	8.747616562097212	71.40407128476623	8.954269948975787	10.894042204160764
12	71.6884859976352	8.523919596919374	7.344717023339049	12.442877382106374
13	9.42722924678036	10.446648273805085	9.852253480618256	70.2738689987963
14	69.61023466876871	9.572099662324105	10.897237875091875	9.920427793815312
15	7.7409802187969365	10.481800654047317	69.45151634585682	12.325702781298933
16	9.04587918233433	11.766460368354336	8.930835028814299	70.25682542049704
17	72.17529320280794	11.340370910872737	7.933785698307359	8.550550188011972
18	12.592008692224931	11.097499920108227	68.01239920321271	8.298092184454125
19	72.0613142729316	11.779243052078783	8.996878894723947	7.162563780265667
20	20.43525038081745	13.176816472618427	58.55960458898346	7.828328557580664
21	13.418622239739234	68.26485720677056	8.530310938781598	9.786209614708607
22	71.00674286566465	13.900103326693438	7.573740106735409	7.5194137009065045
23	72.74092695761475	10.874868178574092	10.310299647410973	6.073905216400184
24	73.21708192635043	12.829553564770924	6.220906079231335	7.732458429647304
25	20.22646654665147	66.10245321005145	7.142324531035291	6.528755712261789
26	14.098234924422382	70.52952267328526	8.223526529394846	7.148715872897515
27	12.81250998647166	71.47118037431959	8.473854085665286	7.242455553543467
28	21.691149056744464	65.99166995110623	5.842751685716417	6.474429306432886
29	17.12773096711655	68.61744623283658	8.101025810368887	6.153796989677983
30	45.635246119922876	39.291839321665584	7.293586288441259	7.77932826997028
31	18.957785187000013	11.698286055157281	62.288952565591146	7.054976192251564
32	73.87006401994098	9.904449439159752	10.26662547801911	5.9588610628801515
33	16.165113182423436	10.18375499334221	67.49613848202397	6.154993342210386
34	14.509411250892123	10.914281453391139	68.6749683095966	5.901338986120136
35	12.767770593436092	11.329718674435698	21.539887299338496	54.36262343278971
36	15.346677034843465	12.480160209636013	7.107172150793059	65.06599060472746
37	16.045463745113288	10.349712922228022	7.552435633861329	66.05238769879736
38	14.728847321495147	11.35421881824089	12.979750098533188	60.93718376173077
39	16.47048797895118	11.34889270002237	7.6110229342650495	64.56959638676139
40	36.692693631027836	19.273091385536393	15.420177466259041	28.614037517176733
41	17.851017821191558	63.161370729784714	6.923953684075972	12.06365776494775
42	31.402793016393794	54.143187362186694	8.499419453114182	5.954600168305336
43	16.93705593489353	13.045793964442835	62.01519008915922	8.001960011504416
44	62.57443250210382	11.536372061314273	19.583071465854257	6.306123970727655
45	15.352003153061986	12.520638708096765	65.40047082885052	6.726887309990732
46	15.100610373147843	22.26636982434462	10.851433258412603	51.781586544094935
47	46.22964091310971	35.40590346943341	8.644289868657925	9.72016574879896
48	13.345121808323658	67.08139373861542	10.665019120764404	8.908465332296515
49	11.17538028889173	10.813200392006477	33.322680983424945	44.68873833567685
50	10.608562267648093	9.62109994993449	10.98884710845042	68.781490673967
51	12.758183580642758	9.862905717055295	33.400087348338786	43.978823353963165
52	10.328408449353942	11.127326182131938	10.911085782460027	67.6331795860541
53	28.404188459367045	16.017767930376984	12.714509411250893	42.86353419900508
54	63.43726365350405	15.189023935575275	11.337175239941626	10.036537170979047
55	12.601595705018267	12.408790225507845	66.36343300275894	8.626181066714956
56	46.72972857812434	11.984958881929355	32.047594699390686	9.237717840555625
57	68.54893689547914	9.62972431718437	11.746346243981423	10.074992543355064
58	11.975244202520319	10.993108003025236	66.49871640550934	10.532931388945109
59	8.518593478700854	11.160348115086762	18.361259946525774	61.95979845968661
60	35.59589245387533	12.616643231496868	38.097064212365254	13.69040010226256
61	10.751416762537817	9.12799863649921	10.754612467510333	69.36597213345263
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	3.0
16	1.5
17	3.0
18	6.0
19	6.0
20	8.5
21	11.0
22	9.0
23	7.0
24	9.0
25	11.5
26	12.0
27	15.5
28	19.0
29	64.5
30	343.5
31	577.0
32	333.5
33	90.0
34	85.0
35	540.0
36	1000.0
37	544.5
38	89.0
39	398.5
40	681.5
41	655.0
42	592.5
43	1209.0
44	1888.0
45	2016.5
46	2145.0
47	2615.0
48	5065.5
49	7046.0
50	8853.0
51	10660.0
52	13312.0
53	17109.5
54	18255.0
55	16564.5
56	14874.0
57	11910.0
58	6474.5
59	4003.0
60	2787.0
61	1063.0
62	555.0
63	440.0
64	325.0
65	250.0
66	144.0
67	113.0
68	97.5
69	82.0
70	76.0
71	61.5
72	53.0
73	45.0
74	37.0
75	27.0
76	14.0
77	11.0
78	8.0
79	5.0
80	4.0
81	3.0
82	3.0
83	3.5
84	3.5
85	3.0
86	2.0
87	1.0
88	1.5
89	1.5
90	1.0
91	0.5
92	0.0
93	1.5
94	2.5
95	2.0
96	2.5
97	3.0
98	4.5
99	29.0
100	52.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0021304472874079917
2	0.0
3	0.0010652236437039958
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0021304472874079917
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0010652236437039958
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0010652236437039958
57	0.0010652236437039958
58	0.0
59	0.0
60	0.0010652236437039958
61	0.0010652236437039958
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	93877.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.72258380647016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.88845756163042	51.023147309777684
2	6.407023781450107	7.524739819125026
3	2.374517024325648	4.183133248825591
4	1.1754675567326356	2.761059684480757
5	0.694759373809567	2.039903277693152
6	0.4553122789195857	1.6042268074182175
7	0.3265187657590654	1.3421817910670346
8	0.24488907431929907	1.1504415352003152
9	0.179585321167486	0.9491142665402602
>10	1.1065358172946107	12.795466408172395
>50	0.0852576777259782	3.5503904044654173
>100	0.05260580115007166	6.773757150313708
>500	0.0072559725724236765	3.1296270652023397
>1k	0.0018139931431059191	1.1728112317180992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	1101	1.1728112317180992	No Hit
TTGATCGAATCGATCGAGGATATCATCATCTGTTAGACTGGTCCAAGACAATTTACTAATT	936	0.9970493305069399	No Hit
TTGCTCATGAAGGTAATGAAGTGAGCTGTGAAGCTTGTAAATGGAGTCCTGAACTAGCTGC	743	0.7914611672720688	No Hit
TAATCGGGTTGCTCTAGAAGCTTGTGTACAAGCTCGTAATGAAGGACGTGATCTTGCTCGT	720	0.7669610234668769	No Hit
AGATCGGAAGAGCGTCGGTGTAGGGAAAGAGTGTTCACTCGAGTGTAGATCTCGGTGGTCG	539	0.5741555439564536	Illumina Single End PCR Primer 1 (95% over 22bp)
CGTTAGAATAGCTTCCATTGAGTCTCTGCACCTATCCCCTTCCTATCTTAGGAGAAGAAAC	500	0.5326118218519978	No Hit
CTGGGATATTTTGAATGGGTAATCCTGAGCCAAATCCGGTTCATGAAGACAATGTTTCTTC	480	0.511307348977918	No Hit
AGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTTCACTCGAGTGTAGATCTCGGTGGTCGC	473	0.50385078347199	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCGTTGTCTC	359	0.38241528808973446	Illumina Single End PCR Primer 1 (96% over 29bp)
GATAGACTACGAGACCAAATGCGTGTAAGACTGTTGTGTTAAGTTAACTAGAGTAGCAAGA	325	0.3461976842037986	No Hit
AAAATAGAAAACTTATTTGACCAATGAAATGCTCTTTGAGTAATTGCCTCGATTCAGAATC	311	0.33128455319194267	No Hit
GTTACTAACTTGTTCACTTCCATTGTAGGTAATGTATTTGGATTCAAGGCCCTAAGATCGG	289	0.30784963303045476	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCGTTGTCGC	282	0.30039306752452677	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCGTGGTCGC	269	0.2865451601563748	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCCAGGAACGGGCTCGATGTCATAGCATCGCCCCTTGTAACGATCGAGACTAGTAAGAGA	249	0.26524068728229494	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCGTGGTCTC	237	0.252458003557847	Illumina Single End PCR Primer 1 (96% over 29bp)
ATGACTATAGAAATAGTCCAGAGAATTTACAAAGATCAATTATTGTCGTCCCAAGAGTAAA	232	0.24713188533932698	No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGG	223	0.23754487254599105	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCGAGTGTCGATCTCGGTGGTCGC	170	0.18108801942967925	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCCAATGTCAACCCCCGTTGTCTC	168	0.17895757214227126	Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGGAATAGCGATAATTATAGTGGCGGACGTAAAGTACGCTCGTGTGTCTACATCTATTC	141	0.15019653376226338	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCCAATGTCAACCCCCGTGGTCTC	139	0.1480660864748554	Illumina Single End PCR Primer 1 (96% over 33bp)
TACGACAAACAGACCTAAAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCAGATC	136	0.1448704155437434	No Hit
CAGAGAGAACGAATTTGGCATTCAGATATTCCCCAGATAAATCAGATCGGAAGAGCGTCGT	135	0.1438051919000394	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCGACCCCCGTTGTCTC	132	0.14060952096892743	Illumina Single End PCR Primer 1 (96% over 29bp)
TGTAGTAAAGCTCCATGGGGTCTTCTCGTCCTGGTACGGACAGACGGCATCTTAGATCGGA	131	0.13954429732522342	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCCAATGTCAACCCCCGTGGTCGC	130	0.13847907368151943	Illumina Single End PCR Primer 1 (96% over 33bp)
GGCTTATGGGAAGGATGTAACCAGCATTTTCGGGGTATGGGCTCGATGGCTTATGGGAAGG	128	0.13634862639411147	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCCAATGTCAACCCCCGTTGTCGC	127	0.13528340275040746	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCGAGTGTCGATCTCGGTGGTCTC	120	0.1278268372444795	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATTTCAACCCCCGTTGTCTC	120	0.1278268372444795	Illumina Single End PCR Primer 1 (96% over 29bp)
ACCGAAGTTTCATCATGATGAGATGTTTGAGGGGGCTGGGAGGTCAATGAATGCGTTGTTG	118	0.1256963899570715	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCGACCCCCGTGGTCGC	118	0.1256963899570715	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCGAATGTCGATCTCGGTGGTCGC	117	0.12463116631336749	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCATGGTCGC	98	0.10439191708299157	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCCAATGTCGACCCCCGTTGTCTC	98	0.10439191708299157	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCGACCCCCGTTGTCGC	96	0.10226146979558358	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCATTGTCTC	94	0.1001310225081756	Illumina Single End PCR Primer 1 (96% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	60.541985789916595	0.0	0.0	0.0	0.0
2	60.83066139736037	0.0	0.0	0.0	0.0
3	60.951031669098924	0.0	0.0	0.0	0.0
4	61.04264090245747	0.0	0.0	0.0	0.0
5	61.21946802731233	0.0	0.0	0.0	0.0
6	61.47938259637611	0.0	0.0	0.0	0.0
7	61.795754018556195	0.0	0.0	0.0	0.0
8	62.60319354048382	0.0	0.0	0.0	0.0
9	63.154979387922495	0.0	0.0	0.0	0.0
10	63.73765672102858	0.0	0.0	0.0	0.0
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>>END_MODULE