FastQCFastQC Report
Mon 8 Jul 2024
AM-AL-EC4_S108_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-EC4_S108_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93877
Sequences flagged as poor quality0
Sequence length61
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG11011.1728112317180992No Hit
TTGATCGAATCGATCGAGGATATCATCATCTGTTAGACTGGTCCAAGACAATTTACTAATT9360.9970493305069399No Hit
TTGCTCATGAAGGTAATGAAGTGAGCTGTGAAGCTTGTAAATGGAGTCCTGAACTAGCTGC7430.7914611672720688No Hit
TAATCGGGTTGCTCTAGAAGCTTGTGTACAAGCTCGTAATGAAGGACGTGATCTTGCTCGT7200.7669610234668769No Hit
AGATCGGAAGAGCGTCGGTGTAGGGAAAGAGTGTTCACTCGAGTGTAGATCTCGGTGGTCG5390.5741555439564536Illumina Single End PCR Primer 1 (95% over 22bp)
CGTTAGAATAGCTTCCATTGAGTCTCTGCACCTATCCCCTTCCTATCTTAGGAGAAGAAAC5000.5326118218519978No Hit
CTGGGATATTTTGAATGGGTAATCCTGAGCCAAATCCGGTTCATGAAGACAATGTTTCTTC4800.511307348977918No Hit
AGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTTCACTCGAGTGTAGATCTCGGTGGTCGC4730.50385078347199Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCGTTGTCTC3590.38241528808973446Illumina Single End PCR Primer 1 (96% over 29bp)
GATAGACTACGAGACCAAATGCGTGTAAGACTGTTGTGTTAAGTTAACTAGAGTAGCAAGA3250.3461976842037986No Hit
AAAATAGAAAACTTATTTGACCAATGAAATGCTCTTTGAGTAATTGCCTCGATTCAGAATC3110.33128455319194267No Hit
GTTACTAACTTGTTCACTTCCATTGTAGGTAATGTATTTGGATTCAAGGCCCTAAGATCGG2890.30784963303045476No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCGTTGTCGC2820.30039306752452677Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCGTGGTCGC2690.2865451601563748Illumina Single End PCR Primer 1 (96% over 29bp)
CTCCAGGAACGGGCTCGATGTCATAGCATCGCCCCTTGTAACGATCGAGACTAGTAAGAGA2490.26524068728229494No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCGTGGTCTC2370.252458003557847Illumina Single End PCR Primer 1 (96% over 29bp)
ATGACTATAGAAATAGTCCAGAGAATTTACAAAGATCAATTATTGTCGTCCCAAGAGTAAA2320.24713188533932698No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGG2230.23754487254599105No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCGAGTGTCGATCTCGGTGGTCGC1700.18108801942967925Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCCAATGTCAACCCCCGTTGTCTC1680.17895757214227126Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGGAATAGCGATAATTATAGTGGCGGACGTAAAGTACGCTCGTGTGTCTACATCTATTC1410.15019653376226338No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCCAATGTCAACCCCCGTGGTCTC1390.1480660864748554Illumina Single End PCR Primer 1 (96% over 33bp)
TACGACAAACAGACCTAAAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCAGATC1360.1448704155437434No Hit
CAGAGAGAACGAATTTGGCATTCAGATATTCCCCAGATAAATCAGATCGGAAGAGCGTCGT1350.1438051919000394No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCGACCCCCGTTGTCTC1320.14060952096892743Illumina Single End PCR Primer 1 (96% over 29bp)
TGTAGTAAAGCTCCATGGGGTCTTCTCGTCCTGGTACGGACAGACGGCATCTTAGATCGGA1310.13954429732522342No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCCAATGTCAACCCCCGTGGTCGC1300.13847907368151943Illumina Single End PCR Primer 1 (96% over 33bp)
GGCTTATGGGAAGGATGTAACCAGCATTTTCGGGGTATGGGCTCGATGGCTTATGGGAAGG1280.13634862639411147No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCCAATGTCAACCCCCGTTGTCGC1270.13528340275040746Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCGAGTGTCGATCTCGGTGGTCTC1200.1278268372444795Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATTTCAACCCCCGTTGTCTC1200.1278268372444795Illumina Single End PCR Primer 1 (96% over 29bp)
ACCGAAGTTTCATCATGATGAGATGTTTGAGGGGGCTGGGAGGTCAATGAATGCGTTGTTG1180.1256963899570715No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCGACCCCCGTGGTCGC1180.1256963899570715Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCGAATGTCGATCTCGGTGGTCGC1170.12463116631336749Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCATGGTCGC980.10439191708299157Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCCCCCCAATGTCGACCCCCGTTGTCTC980.10439191708299157Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCGACCCCCGTTGTCGC960.10226146979558358Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCCCCCCAATGTCAACCCCCATTGTCTC940.1001310225081756Illumina Single End PCR Primer 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)