##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-EC4_S108_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 93877 Sequences flagged as poor quality 0 Sequence length 61 %GC 57 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.1015477699543 40.0 40.0 40.0 40.0 40.0 2 38.953694728208184 40.0 40.0 40.0 40.0 40.0 3 38.78097936661802 40.0 40.0 40.0 40.0 40.0 4 39.06464842293639 40.0 40.0 40.0 40.0 40.0 5 38.64831641403113 40.0 40.0 40.0 40.0 40.0 6 38.83826709417642 40.0 40.0 40.0 40.0 40.0 7 39.04104306699192 40.0 40.0 40.0 40.0 40.0 8 38.849558464799685 40.0 40.0 40.0 40.0 40.0 9 39.1339305687229 40.0 40.0 40.0 40.0 40.0 10 39.126303567433986 40.0 40.0 40.0 40.0 40.0 11 39.20410750237012 40.0 40.0 40.0 40.0 40.0 12 39.187063924070856 40.0 40.0 40.0 40.0 40.0 13 38.619427548813874 40.0 40.0 40.0 40.0 40.0 14 39.22409109792601 40.0 40.0 40.0 40.0 40.0 15 39.416853968490685 40.0 40.0 40.0 40.0 40.0 16 39.173855150888926 40.0 40.0 40.0 40.0 40.0 17 39.28591667820659 40.0 40.0 40.0 40.0 40.0 18 39.27598879384727 40.0 40.0 40.0 40.0 40.0 19 39.35553969555908 40.0 40.0 40.0 40.0 40.0 20 37.84339081990264 40.0 40.0 40.0 24.0 40.0 21 39.41693918638218 40.0 40.0 40.0 40.0 40.0 22 39.25003994588664 40.0 40.0 40.0 40.0 40.0 23 39.25259648263153 40.0 40.0 40.0 40.0 40.0 24 39.28152795679453 40.0 40.0 40.0 40.0 40.0 25 39.55891219361505 40.0 40.0 40.0 40.0 40.0 26 39.409951319279486 40.0 40.0 40.0 40.0 40.0 27 39.36734237353132 40.0 40.0 40.0 40.0 40.0 28 39.276031402793016 40.0 40.0 40.0 40.0 40.0 29 39.236745954813216 40.0 40.0 40.0 40.0 40.0 30 39.233166803370366 40.0 40.0 40.0 40.0 40.0 31 39.35076749363529 40.0 40.0 40.0 40.0 40.0 32 39.42584445604355 40.0 40.0 40.0 40.0 40.0 33 39.199889216741056 40.0 40.0 40.0 40.0 40.0 34 39.377398084727886 40.0 40.0 40.0 40.0 40.0 35 39.1977161605079 40.0 40.0 40.0 40.0 40.0 36 39.1687846863449 40.0 40.0 40.0 40.0 40.0 37 39.22455979632924 40.0 40.0 40.0 40.0 40.0 38 39.35553969555908 40.0 40.0 40.0 40.0 40.0 39 39.344802241230546 40.0 40.0 40.0 40.0 40.0 40 39.17313079881121 40.0 40.0 40.0 40.0 40.0 41 35.81209454925062 40.0 40.0 40.0 24.0 40.0 42 39.15284894063509 40.0 40.0 40.0 40.0 40.0 43 39.049564856141544 40.0 40.0 40.0 40.0 40.0 44 38.89855875241007 40.0 40.0 40.0 40.0 40.0 45 28.521277842282988 24.0 24.0 40.0 12.0 40.0 46 32.60696443218254 40.0 24.0 40.0 24.0 40.0 47 38.054390319247524 40.0 40.0 40.0 40.0 40.0 48 38.59087955516261 40.0 40.0 40.0 40.0 40.0 49 38.68496010737454 40.0 40.0 40.0 40.0 40.0 50 38.61955537565112 40.0 40.0 40.0 40.0 40.0 51 38.534167048371806 40.0 40.0 40.0 40.0 40.0 52 38.34498332924998 40.0 40.0 40.0 40.0 40.0 53 38.054859017650756 40.0 40.0 40.0 40.0 40.0 54 37.64888098256229 40.0 40.0 40.0 24.0 40.0 55 37.328802582102114 40.0 40.0 40.0 24.0 40.0 56 36.94983861861798 40.0 40.0 40.0 24.0 40.0 57 36.388380540494474 40.0 40.0 40.0 24.0 40.0 58 35.57143922366501 40.0 40.0 40.0 24.0 40.0 59 34.20910340125909 40.0 24.0 40.0 24.0 40.0 60 33.01605292031062 40.0 24.0 40.0 12.0 40.0 61 32.06855779370879 40.0 24.0 40.0 12.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.032451806396139204 1101 2 0.2656984087454006 1101 3 0.47382403704123277 1101 4 0.19374548253096435 1101 5 0.21352069913015725 1101 6 -0.6324326622231808 1101 7 -0.044322937955072916 1101 8 -0.14134340479016316 1101 9 0.2928243297840112 1101 10 0.11100466841386947 1101 11 0.38091394461794437 1101 12 0.041825272366743604 1101 13 -0.043272685100831154 1101 14 0.02890060960083929 1101 15 -0.17228374965461057 1101 16 -0.2315496052758803 1101 17 0.11544144715409033 1101 18 -0.0023826151327241973 1101 19 -0.07193899953578153 1101 20 -0.030868733273919702 1101 21 0.18107931899211138 1101 22 0.16743425461418582 1101 23 0.10806469655625506 1101 24 0.1470052595549305 1101 25 0.06094076975676899 1101 26 -0.16664766279910026 1101 27 -0.024572233949982092 1101 28 0.1481394228655475 1101 29 -0.39581858075831633 1101 30 -0.11516153191239908 1101 31 -0.011598520055287054 1101 32 0.062224285723509354 1101 33 -0.49622141602549164 1101 34 0.027212195987324606 1101 35 0.003981251025663823 1101 36 0.23216720377337197 1101 37 0.024525590844234557 1101 38 0.050784932443406205 1101 39 -0.2235212901215604 1101 40 -0.04614104046535061 1101 41 0.4440694255011479 1101 42 -0.28162595610349683 1101 43 -0.009977190473627218 1101 44 -0.05377138830144901 1101 45 -0.3983779984297726 1101 46 1.001788798163986 1101 47 -0.1439117416459723 1101 48 -0.02157289486846281 1101 49 -0.3227940223140564 1101 50 -0.2494091600057189 1101 51 0.09686127466147809 1101 52 0.2237964585110248 1101 53 -0.15377820895597694 1101 54 0.506603232857934 1101 55 0.46865568178045436 1101 56 -0.10484381125680642 1101 57 -0.09198804043575848 1101 58 -0.6737685793513322 1101 59 -0.7767086568811337 1101 60 -0.30804717240373236 1101 61 0.5981719059418751 1102 1 0.2929315247964439 1102 2 0.20179145080697936 1102 3 -0.15539906650136004 1102 4 -0.25372214182835506 1102 5 -0.005860685322396364 1102 6 0.04183687910704492 1102 7 0.2690518583965016 1102 8 0.20727892102353707 1102 9 0.08400293391525082 1102 10 0.09587819828032451 1102 11 -0.14475467628084004 1102 12 0.04006527426944473 1102 13 0.14975629540838042 1102 14 0.054444365769747094 1102 15 0.0759711170968842 1102 16 -0.07262653384136541 1102 17 0.020401549899929705 1102 18 -0.11449925068230726 1102 19 0.010210100843572434 1102 20 0.24488015780836037 1102 21 0.04765243621036319 1102 22 -0.4302040182093023 1102 23 -0.18323877230486119 1102 24 -0.4639994853048037 1102 25 -0.3091345058375268 1102 26 0.19282005669525404 1102 27 -0.4418820530745009 1102 28 -0.46315072709104044 1102 29 -0.35010619774467955 1102 30 -0.23098843372115851 1102 31 -0.20676803879094763 1102 32 -0.3377233900087333 1102 33 -0.0070846475246995055 1102 34 -0.36108143828982264 1102 35 0.11314870057436366 1102 36 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2304 15 -0.28211422019676746 2304 16 -0.34703105774608645 2304 17 -0.25651625440692527 2304 18 0.18566639734419255 2304 19 -0.25277042123331483 2304 20 -2.018671564359913 2304 21 -0.190766481936663 2304 22 0.1151207090426496 2304 23 -0.19289205384938413 2304 24 -0.1621202370041246 2304 25 -0.07865526896085129 2304 26 0.13061636674266452 2304 27 -0.36379900058120995 2304 28 -0.3112686227899104 2304 29 -0.41299532780693937 2304 30 -0.22504795277068013 2304 31 -0.24429588479539888 2304 32 -0.16213648191482832 2304 33 -0.43971159674501337 2304 34 -0.005966341471406622 2304 35 -0.34900929337091213 2304 36 -0.48410874318572894 2304 37 -0.3311505687260663 2304 38 -0.07532708008946543 2304 39 -0.23230548975229226 2304 40 -0.28443343681739464 2304 41 0.08621120360219692 2304 42 0.11103336242165085 2304 43 -0.33303431574638864 2304 44 -0.6502017581330364 2304 45 0.39136071359395075 2304 46 -1.7319995977404474 2304 47 -0.5470337692854272 2304 48 -0.33399946007855874 2304 49 -0.7146140860974128 2304 50 -0.49368824018252155 2304 51 -0.7155373153905558 2304 52 -0.9055606723567706 2304 53 0.39945851683152256 2304 54 0.32498850673473356 2304 55 0.04365708053835249 2304 56 -1.289983407295523 2304 57 -0.07811236203816918 2304 58 -1.1389395075670734 2304 59 -0.5024961575805165 2304 60 -0.3048580043849327 2304 61 -0.18552417194096193 2401 1 -0.0017227939271933224 2401 2 0.055037370484221526 2401 3 -0.07746031718905755 2401 4 0.18094684771867975 2401 5 0.6370881501809933 2401 6 0.1807970844985718 2401 7 0.031705443578971426 2401 8 0.45920446659478387 2401 9 0.1586407479625649 2401 10 -0.15228794879467955 2401 11 -0.06232298730229502 2401 12 -0.10205832741769427 2401 13 0.6270165191384365 2401 14 0.04389971145102578 2401 15 0.027419860907627935 2401 16 0.16224417952742698 2401 17 0.2930954798467411 2401 18 0.16754732918198556 2401 19 -0.005790265927011262 2401 20 0.20458123978158937 2401 21 -0.1340365423335541 2401 22 0.03078076081141745 2401 23 -0.041252709587091374 2401 24 0.11462695885437313 2401 25 -0.030984863438845878 2401 26 -0.598029017209015 2401 27 -0.26500693845869705 2401 28 0.06362352561389173 2401 29 0.04041278263309778 2401 30 -0.004599290130471445 2401 31 0.01260661736163371 2401 32 -0.09203294438247411 2401 33 -0.01607052685717747 2401 34 0.021643667156716617 2401 35 0.17687508971795296 2401 36 -0.08721486915639076 2401 37 0.2870547807131061 2401 38 -0.031755035223199 2401 39 -0.03450566231484231 2401 40 0.24813774523610022 2401 41 1.0853483908325643 2401 42 0.3256580388668624 2401 43 0.06407526147535947 2401 44 0.2650268872509116 2401 45 0.34520023041880066 2401 46 -0.0609469661615023 2401 47 0.1451577751494355 2401 48 -0.1817130062390433 2401 49 -8.522224218339147E-4 2401 50 0.138322113570716 2401 51 -0.06476078389788853 2401 52 0.08947815421786487 2401 53 -0.4344500250148968 2401 54 -0.17522719645767637 2401 55 0.10081842652627415 2401 56 -0.007843631626840875 2401 57 0.278229311818599 2401 58 0.7443650653406024 2401 59 -0.28959710667456307 2401 60 0.8388003217582991 2401 61 0.6397562422091667 2402 1 0.23067525417318535 2402 2 0.1283207444821315 2402 3 -0.3775648832399412 2402 4 0.3216753245398749 2402 5 0.3937978051130244 2402 6 0.3335506571719762 2402 7 -0.027461513548949767 2402 8 0.6133522532800413 2402 9 0.04745218051746036 2402 10 1.1707037576513812E-4 2402 11 0.0772726906342669 2402 12 -0.028513538292564533 2402 13 0.5954724271203133 2402 14 0.29050131479714025 2402 15 0.24866419691321084 2402 16 -0.08061047366184226 2402 17 -0.1464304873892246 2402 18 0.12432669481461289 2402 19 0.0030107100227922956 2402 20 0.7614825991402512 2402 21 0.09975571977867759 2402 22 -0.013746949192068314 2402 23 0.139210866571851 2402 24 0.17693089750896007 2402 25 0.2176209225493011 2402 26 0.28119719401441046 2402 27 0.497453849272226 2402 28 0.4698626333169216 2402 29 0.32709803881991917 2402 30 0.400942710566639 2402 31 0.4984030621158979 2402 32 0.3144675784477826 2402 33 0.45552236265136514 2402 34 0.25622017465062186 2402 35 0.42452235357296075 2402 36 0.40982242606842334 2402 37 0.23111194348759057 2402 38 0.043009691162296804 2402 39 0.17297081032370443 2402 40 0.36359609309249663 2402 41 0.3617513186819892 2402 42 0.3353303985740794 2402 43 0.38936281811530193 2402 44 0.15340262792710746 2402 45 0.4175250822835608 2402 46 0.1024374883522654 2402 47 0.37110758344500283 2402 48 0.03317022833746819 2402 49 0.38787207175732163 2402 50 0.5274820996285783 2402 51 0.5212447929581572 2402 52 0.4238281019348378 2402 53 1.0334830527125405 2402 54 0.2713569792132944 2402 55 0.42819730418399615 2402 56 1.0852201536642028 2402 57 1.064217460999501 2402 58 0.9987219841276413 2402 59 1.378841373632163 2402 60 0.14883866264362666 2402 61 1.8762497939693645 2403 1 -0.6434969312572747 2403 2 -0.6465136926039676 2403 3 -0.19213441966378753 2403 4 -0.3803114304270139 2403 5 0.43353290445077164 2403 6 0.21434535915872743 2403 7 -0.014216480436367362 2403 8 -0.14161463413717712 2403 9 -0.20420344862161244 2403 10 -0.11908822763748361 2403 11 -0.002197508041227536 2403 12 -0.29341420054421974 2403 13 -0.05353419539624582 2403 14 -0.23930000970617016 2403 15 0.1427039320125445 2403 16 -0.1733257054499191 2403 17 -0.09345035493889498 2403 18 -0.2995143647880383 2403 19 -0.06321445554011973 2403 20 0.44360180443825925 2403 21 -0.29759527359880167 2403 22 -0.30513767766888833 2403 23 0.04649563478376706 2403 24 -0.15419493030560716 2403 25 0.32358118744996744 2403 26 -0.02343856757499907 2403 27 0.1928180876828165 2403 28 0.032776540601688 2403 29 -0.07025295455756009 2403 30 0.1492870814275662 2403 31 -0.004908196162247691 2403 32 0.07605698242129222 2403 33 0.21711176662487475 2403 34 0.004564545511549056 2403 35 0.15962169132130555 2403 36 -0.34514446134879506 2403 37 0.12515167858693133 2403 38 -0.274871103539688 2403 39 0.1332357109859572 2403 40 -0.35163569498697456 2403 41 0.8650625769601348 2403 42 -0.009040462353070211 2403 43 0.2701575201020532 2403 44 -0.3101735310132909 2403 45 -0.2712166395707456 2403 46 -1.2220658229060106 2403 47 -0.09246857549538845 2403 48 0.006680162112303378 2403 49 -0.08894912029565916 2403 50 -0.3996702182522185 2403 51 0.16362889891842514 2403 52 -0.15895335501880226 2403 53 0.19904596661982055 2403 54 0.0859265156371336 2403 55 -0.04862388786898464 2403 56 -0.29226329004441 2403 57 0.097330043780957 2403 58 0.07156966624684458 2403 59 -0.3165228647784275 2403 60 -0.36771762874710845 2403 61 -1.0641475570240146 2404 1 -0.17382805708508897 2404 2 -0.39741876986665403 2404 3 0.2649958231618186 2404 4 -0.3757198189479851 2404 5 -0.10606974455584606 2404 6 -0.16499238918564174 2404 7 -0.1407506967719101 2404 8 -0.017111322878903934 2404 9 -0.06521890116024309 2404 10 -0.04544584353152459 2404 11 0.07206297760998126 2404 12 0.312678514687569 2404 13 0.36350774720861523 2404 14 0.1803909395212031 2404 15 0.299525124065525 2404 16 0.0732968111063741 2404 17 0.4481831991449923 2404 18 0.06719645198900537 2404 19 0.1985956989852653 2404 20 -0.28752402337630656 2404 21 0.0833318787190791 2404 22 0.3411316380043985 2404 23 0.04208062374624433 2404 24 0.36006555534560647 2404 25 0.06954145235062725 2404 26 -0.054520245279192636 2404 27 0.19008078083955127 2404 28 0.37485159578933036 2404 29 0.43093909842257005 2404 30 0.26522527127303874 2404 31 0.12401012613356244 2404 32 0.2863881082491062 2404 33 0.20006982402001938 2404 34 0.21392436891110833 2404 35 0.2888049142793605 2404 36 0.5134868852295753 2404 37 0.16108986843240558 2404 38 0.025437947232937574 2404 39 0.4765469692641062 2404 40 0.5472605522536469 2404 41 0.33517295223607135 2404 42 0.18513172307739012 2404 43 0.4789875421771086 2404 44 0.44029004514565173 2404 45 0.7041475988398531 2404 46 0.9480004022595523 2404 47 0.813403389184522 2404 48 0.2509185727083292 2404 49 0.5284460231922026 2404 50 0.2551642188338761 2404 51 0.4750637775056177 2404 52 -0.5573639510452892 2404 53 0.3876552381429974 2404 54 0.5372289438932043 2404 55 0.26660790021048797 2404 56 0.7128581227591226 2404 57 0.3540187855028094 2404 58 -0.2368630048348308 2404 59 -0.14380763299035237 2404 60 -5.88365581859258 2404 61 -5.64410340691364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 2.0 17 15.0 18 30.0 19 34.0 20 34.0 21 54.0 22 54.0 23 88.0 24 145.0 25 186.0 26 278.0 27 341.0 28 417.0 29 520.0 30 671.0 31 828.0 32 1051.0 33 1231.0 34 1479.0 35 1988.0 36 3296.0 37 7186.0 38 28198.0 39 37999.0 40 7752.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.515397807769741 71.57557229140258 11.66845979313357 8.24057010769411 2 72.40600462385869 9.228540075217609 8.77041582766005 9.595039473263656 3 8.94603404563564 73.12461384408888 10.709035515691244 7.220316594584229 4 8.00835135336664 8.678377025256452 73.93504266220693 9.378228959169977 5 9.626426068153009 7.91248122543328 11.477784760910554 70.98330794550316 6 71.38063636460474 9.433620588642585 9.268510923868465 9.917232122884199 7 73.70921524974169 8.00089478786071 10.644779871534029 7.645110090863577 8 7.4405871512724095 81.37456458983564 4.214024734493007 6.970823524398948 9 16.56635810688454 71.13670015019653 9.08848812808249 3.208453614836435 10 70.11195500495329 17.25875347529214 6.959106064318203 5.670185455436369 11 8.132982519680008 69.66988719281613 17.21294885861287 4.984181428890996 12 69.45471201678792 6.967627853467835 8.010481800654047 15.567178329090192 13 17.25129690978621 8.420592903480086 4.855289368002812 69.4728208187309 14 16.13174686025331 71.01526465481427 5.914121669844584 6.938866815087828 15 7.844306912236224 19.260308701811944 6.270971590485422 66.6244127954664 16 8.26933114607412 78.23002439362145 5.543423841835594 7.957220618468848 17 16.261704144785195 9.548664742162618 4.581526891570886 69.6081042214813 18 70.05017203361847 16.778337611981637 7.5811966722413375 5.590293682158569 19 14.658542561010684 7.783589164545096 68.53222834134026 9.025639933103955 20 10.868476836711867 10.45303961566731 4.389786635704166 74.28869691191665 21 13.61462339018077 8.271461593361526 69.75084418973763 8.36307082672007 22 70.84589409546534 10.265560254375405 9.95984106863236 8.928704581526892 23 10.667149568051812 73.13399448214153 5.794816621749736 10.404039328056927 24 13.226881983872513 72.82507962546737 5.810794976405297 8.137243414254822 25 11.457545511680177 12.056201199441823 7.88052451612216 68.60572877275584 26 10.247451452432438 10.742780446754796 69.48240783152423 9.527360269288538 27 11.567263546981689 12.632487190685685 5.883230184177168 69.91701907815546 28 9.447468496010737 13.650840994066705 6.376428731212118 70.52526177871043 29 12.8231622229087 71.53828946387294 7.600370697828009 8.038177615390351 30 71.81524761123598 12.736879107768676 5.300552851071083 10.147320429924262 31 9.245076003706979 14.631911969918084 68.5940113126751 7.529000713699842 32 9.936406148470873 11.878308850943256 7.737784547865824 70.44750045272005 33 9.989667330656072 71.15906984671432 7.755893349808793 11.095369472820819 34 8.679442248900157 12.408790225507845 8.558006753517901 70.3537607720741 35 11.349957923666073 70.30582570810742 7.427804467547962 10.916411900678549 36 9.426164023136657 73.03279823598965 7.263760026417547 10.27727771445615 37 8.307679197247463 14.058821649605335 7.070954546907123 70.56254460624008 38 8.609137488415692 12.064722988591454 8.147895650691863 71.178243872301 39 8.697551050843124 13.565623102570385 66.9162840738413 10.820541772745187 40 9.28022838394921 70.71487158728975 8.370527392225997 11.63437263653504 41 8.219265634820031 12.772031488010906 7.575870554022818 71.43283232314624 42 68.50240207931655 12.263919809964102 7.072019770550828 12.161658340168518 43 8.742290443878693 73.56008393962313 6.921823236788565 10.77580237970962 44 7.914611672720688 11.165674233305282 68.26485720677056 12.654856887203469 45 8.217135187532621 11.84209124705732 9.380359406457387 70.56041415895267 46 8.026460155309607 12.266050257251509 68.3575316637728 11.349957923666073 47 67.08245896225912 11.71426440981284 6.95271472245598 14.250561905472054 48 68.25846586490833 9.994993448874592 9.0107268020921 12.735813884124973 49 68.06033426717939 11.99441822810699 6.162318778827615 13.782928725886002 50 70.32286928640669 9.807514087582687 7.443782822203522 12.42583380380711 51 67.85687655123192 12.206397733204085 9.022444262172844 10.914281453391139 52 68.33303151996762 11.54063295588909 6.460581399064734 13.66575412507856 53 68.76657754295515 11.32226210892977 8.68583359076238 11.225326757352706 54 69.91062773629324 11.505480575646857 8.210743845670398 10.37314784238951 55 68.76551231931144 14.24417056360983 6.852583699947804 10.137733417130928 56 70.67971920704753 9.368641946376641 6.42223334789139 13.529405498684449 57 70.47626149110006 10.856759376631125 7.318086432246449 11.34889270002237 58 71.2623965401536 11.195500495328995 7.30743419580941 10.23466876870799 59 72.7260138266029 9.8373403496064 7.038997837596003 10.3976479861947 60 73.35130010545714 8.97877009278098 6.978280089904876 10.691649711857004 61 74.34195809410186 7.407565218317585 8.447223494572684 9.80325319300787 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 1.0 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 1.0 22 5.0 23 9.0 24 12.0 25 14.0 26 13.0 27 22.0 28 31.0 29 202.0 30 256.0 31 139.0 32 167.0 33 195.0 34 122.0 35 162.0 36 275.0 37 192.5 38 110.0 39 406.5 40 1269.0 41 1835.0 42 1189.0 43 1043.5 44 1544.0 45 1133.0 46 722.0 47 1952.5 48 3500.5 49 3818.0 50 4834.0 51 5850.0 52 6042.5 53 5105.5 54 3976.0 55 3044.5 56 2113.0 57 1757.5 58 1344.0 59 1286.0 60 2702.5 61 27940.5 62 51762.0 63 27331.5 64 2901.0 65 1581.0 66 189.5 67 118.0 68 102.0 69 86.0 70 78.5 71 61.0 72 51.0 73 48.5 74 46.0 75 31.0 76 13.5 77 11.0 78 7.5 79 4.0 80 2.5 81 1.0 82 1.0 83 3.0 84 2.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 1.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.017043578299263933 3 0.003195670931111987 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 61 93877.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.304068089095304 #Duplication Level Percentage of deduplicated Percentage of total 1 55.717964780399534 8.527115267850485 2 19.71880002784158 6.035557165226839 3 11.282800863089024 5.180182579332531 4 5.818890512981137 3.5621078645461615 5 2.7563165587805387 2.1091428145339113 6 1.3781582793902694 1.2654856887203467 7 0.7238811164474143 0.7754828126165088 8 0.3967425349759866 0.485741981529022 9 0.2366534419155008 0.32595843497342264 >10 1.5382473724507553 4.716810294321292 >50 0.18097027911185357 1.923793900529416 >100 0.20185146516322125 6.4978642265943725 >500 0.03480197675227953 4.208698616274487 >1k 0.006960395350455906 1.1738764553618033 >5k 0.0 0.0 >10k+ 0.006960395350455906 53.212181897589396 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGGG 49954 53.212181897589396 TruSeq Adapter, Index 1 (97% over 36bp) TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC 1102 1.1738764553618033 No Hit AAATTCTGTGACATATCAGGGCGGCCAATTAGTAAATTGTCTTGGACCAGTCTAACAGATG 922 0.9821361994950839 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTACGATCTGGGGGGGGGGGGGGG 844 0.8990487552861723 TruSeq Adapter, Index 7 (97% over 35bp) AAATTTGATCTCCTTCCATACTTCACAAGCAGCAGCTAGTTCAGGACTCCATTTACAAGCT 758 0.8074395219276286 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATTTGGGGGGGGGGGGGGG 723 0.7701566943979888 TruSeq Adapter, Index 1 (97% over 36bp) TCACGAGCAAGATCACGTCCTTCATTACGAGCTTGTACACAAGCTTCTAGAGCAACCCGAT 704 0.7499174451676129 No Hit CCTTCCTATCTTAGGAGAAGAAACATTGTCTTCATGAACCGGATTTGGCTCAGGATTACCC 489 0.5208943617712539 No Hit TGAAGACAATGTTTCTTCTCCTAAGATAGGAAGGGGATAGGTGCAGAGACTCAATGGAAGC 475 0.5059812307593979 No Hit TGTAATGTATCTATTTCCTATTAGTTCTCGGATAGAAAAAGAAAAATGATTCTGAATCGAG 324 0.3451324605600946 No Hit TGCTACTCTAGTTAACTTAACACAACAGTCTTACACGCATTTGGTCTCGTAGTCTATCAGA 322 0.3430020132726866 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGGT 317 0.3376758950541666 TruSeq Adapter, Index 1 (97% over 36bp) CGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGGG 292 0.31104530396156677 TruSeq Adapter, Index 1 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATGTGGGGGGGGGGGGGGG 285 0.3035887384556388 TruSeq Adapter, Index 1 (97% over 36bp) TAGGGCCTTGAATCCAAATACATTACCTACAATGGAAGTGAACAAGTTAGTAACAGATCGG 285 0.3035887384556388 No Hit CTTACTAGTCTCGATCGTTACAAGGGGCGATGCTATGACATCGAGCCCGTTCCTGGAGAGA 244 0.25991456906377497 No Hit ATTCAACAGGAACAGGAAGAATCTCGTATTTACTCTTGGGACGACAATAATTGATCTTTGT 227 0.241805767120807 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCGGGGGGGGGGGGGGGG 222 0.23647964890228707 TruSeq Adapter, Index 1 (97% over 36bp) GGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGA 221 0.23541442525858305 No Hit GGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGGG 195 0.20771861052227913 TruSeq Adapter, Index 1 (97% over 36bp) TCGACCCCGCCGGAGGAGGAGACCCCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA 167 0.17789234849856728 Illumina Multiplexing PCR Primer 2.01 (100% over 33bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTAACAACCTACGATCTGGGGGGGGGGGGGGG 167 0.17789234849856728 Illumina Multiplexing PCR Primer 2.01 (97% over 34bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGTG 162 0.1725662302800473 TruSeq Adapter, Index 1 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCTACGATCTGGGGGGGGGGGGGGG 156 0.16617488841782332 TruSeq Adapter, Index 9 (97% over 37bp) AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGG 145 0.15445742833707937 TruSeq Adapter, Index 1 (97% over 36bp) CCATATGTTTACCGTAGGAATAGATGTAGACACACGAGCGTACTTTACGTCCGCCACTATA 142 0.1512617574059674 No Hit GGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTTTAGGTCTGTTTGTCGTAAGATC 140 0.1491313101185594 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAATCACAACCTACGATCTGGGGGGGGGGGGGGG 137 0.1459356391874474 Illumina Multiplexing PCR Primer 2.01 (97% over 34bp) AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCT 135 0.1438051919000394 TruSeq Adapter, Index 1 (97% over 36bp) GATTTATCTGGGGAATATCTGAATGCCAAATTCGTTCTCTCTGAGATCGGAAGAGCACACG 132 0.14060952096892743 No Hit AAGATGCCGTCTGTCCGTACCAGGACGAGAAGACCCCATGGAGCTTTACTACAAGATCGGA 129 0.13741385003781545 No Hit AGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGG 127 0.13528340275040746 TruSeq Adapter, Index 1 (97% over 36bp) GTTACATCCTTCCCATAAGCCATCGAGCCCATACCCCGAAAATGCTGGTTACATCCTTCCC 124 0.13208773181929548 No Hit AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGG 122 0.12995728453188748 TruSeq Adapter, Index 1 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTAAGATCTGGGGGGGGGGGGGGG 109 0.11610937716373554 TruSeq Adapter, Index 1 (97% over 36bp) TAATGACCAACATCCGAAAATCACACCCACTAATAAAAATTATCAACAACGCATTCATTGA 108 0.11504415352003154 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 61.20881579087529 0.0 0.0 0.0 0.0 2 61.549687356860574 0.0 0.0 0.0 0.0 3 61.7691234274636 0.0 0.0 0.0 0.0 4 62.006668300009586 0.0 0.0 0.0 0.0 5 62.36245299700672 0.0 0.0 0.0 0.0 6 62.96750002663059 0.0 0.0 0.0 0.0 7 63.70037389349894 0.0 0.0 0.0 0.0 8 69.83606208123396 0.0 0.0 0.0 0.0 9 70.61580578842528 0.0 0.0 0.0 0.0 10 71.2080701343247 0.0 0.0 0.0 0.0 11 71.74494285075151 0.0 0.0 0.0 0.0 12 72.4874037304132 0.0 0.0 0.0 0.0 13 73.42373531322902 0.0 0.0 0.0 0.0 14 74.37391480341297 0.0 0.0 0.0 0.0 15 75.64366138670815 0.0 0.0 0.0 0.0 16 77.0742567402026 0.0 0.0 0.0 0.0 17 77.95732714083321 0.0 0.0 0.0 0.0 18 78.36424257272814 0.0 0.0 0.0 0.0 19 79.09605121595278 0.0 0.0 0.0 0.0 20 79.46355337303066 0.0 0.0 0.0 0.0 21 79.76714211148631 0.0 0.0 0.0 0.0 22 80.09842666467824 0.0 0.0 0.0 0.0 23 80.46166792718131 0.0 0.0 0.0 0.0 24 80.7247781671762 0.0 0.0 0.0 0.0 25 81.03156257656295 0.0 0.0 0.0 0.0 26 81.33302086773118 0.0 0.0 0.0 0.0 27 81.58973976586384 0.0 0.0 0.0 0.0 28 81.8709588078017 0.0 0.0 0.0 0.0 29 82.28426558155884 0.0 0.0 0.0 0.0 30 82.57933253086486 0.0 0.0 0.0 0.0 31 82.87652992745826 0.0 0.0 0.0 0.0 32 83.28877147757171 0.0 0.0 0.0 0.0 33 83.67118676566145 0.0 0.0 0.0 0.0 34 83.87997059982743 0.0 0.0 0.0 0.0 35 84.1111241305112 0.0 0.0 0.0 0.0 36 84.2943425972283 0.0 0.0 0.0 0.0 37 84.4562565910713 0.0 0.0 0.0 0.0 38 84.6181705849143 0.0 0.0 0.0 0.0 39 84.68741012175506 0.0 0.0 0.0 0.0 40 84.7790193551136 0.0 0.0 0.0 0.0 41 84.88873739041512 0.0 0.0 0.0 0.0 42 85.04958616061442 0.0 0.0 0.0 0.0 43 85.19765224708928 0.0 0.0 0.0 0.0 44 85.50337143283232 0.0 0.0 0.0 0.0 45 86.83809665839343 0.0 0.0 0.0 0.0 46 87.15979419879204 0.0 0.0 0.0 0.0 47 87.23542507749502 0.0 0.0 0.0 0.0 48 87.415447873281 0.0 0.0 0.0 0.0 49 87.48575263376546 0.0 0.0 0.0 0.0 50 87.53475292137584 0.0 0.0 0.0 0.0 >>END_MODULE