FastQCFastQC Report
Mon 8 Jul 2024
AM-AL-EC4_S108_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-EC4_S108_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93877
Sequences flagged as poor quality0
Sequence length61
%GC57

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGGG4995453.212181897589396TruSeq Adapter, Index 1 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC11021.1738764553618033No Hit
AAATTCTGTGACATATCAGGGCGGCCAATTAGTAAATTGTCTTGGACCAGTCTAACAGATG9220.9821361994950839No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTACGATCTGGGGGGGGGGGGGGG8440.8990487552861723TruSeq Adapter, Index 7 (97% over 35bp)
AAATTTGATCTCCTTCCATACTTCACAAGCAGCAGCTAGTTCAGGACTCCATTTACAAGCT7580.8074395219276286No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATTTGGGGGGGGGGGGGGG7230.7701566943979888TruSeq Adapter, Index 1 (97% over 36bp)
TCACGAGCAAGATCACGTCCTTCATTACGAGCTTGTACACAAGCTTCTAGAGCAACCCGAT7040.7499174451676129No Hit
CCTTCCTATCTTAGGAGAAGAAACATTGTCTTCATGAACCGGATTTGGCTCAGGATTACCC4890.5208943617712539No Hit
TGAAGACAATGTTTCTTCTCCTAAGATAGGAAGGGGATAGGTGCAGAGACTCAATGGAAGC4750.5059812307593979No Hit
TGTAATGTATCTATTTCCTATTAGTTCTCGGATAGAAAAAGAAAAATGATTCTGAATCGAG3240.3451324605600946No Hit
TGCTACTCTAGTTAACTTAACACAACAGTCTTACACGCATTTGGTCTCGTAGTCTATCAGA3220.3430020132726866No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGGT3170.3376758950541666TruSeq Adapter, Index 1 (97% over 36bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGGG2920.31104530396156677TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATGTGGGGGGGGGGGGGGG2850.3035887384556388TruSeq Adapter, Index 1 (97% over 36bp)
TAGGGCCTTGAATCCAAATACATTACCTACAATGGAAGTGAACAAGTTAGTAACAGATCGG2850.3035887384556388No Hit
CTTACTAGTCTCGATCGTTACAAGGGGCGATGCTATGACATCGAGCCCGTTCCTGGAGAGA2440.25991456906377497No Hit
ATTCAACAGGAACAGGAAGAATCTCGTATTTACTCTTGGGACGACAATAATTGATCTTTGT2270.241805767120807No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCGGGGGGGGGGGGGGGG2220.23647964890228707TruSeq Adapter, Index 1 (97% over 36bp)
GGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGA2210.23541442525858305No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGGG1950.20771861052227913TruSeq Adapter, Index 1 (97% over 36bp)
TCGACCCCGCCGGAGGAGGAGACCCCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1670.17789234849856728Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTAACAACCTACGATCTGGGGGGGGGGGGGGG1670.17789234849856728Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGTG1620.1725662302800473TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCTACGATCTGGGGGGGGGGGGGGG1560.16617488841782332TruSeq Adapter, Index 9 (97% over 37bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGGGGGGGGG1450.15445742833707937TruSeq Adapter, Index 1 (97% over 36bp)
CCATATGTTTACCGTAGGAATAGATGTAGACACACGAGCGTACTTTACGTCCGCCACTATA1420.1512617574059674No Hit
GGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTTTAGGTCTGTTTGTCGTAAGATC1400.1491313101185594No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAATCACAACCTACGATCTGGGGGGGGGGGGGGG1370.1459356391874474Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCT1350.1438051919000394TruSeq Adapter, Index 1 (97% over 36bp)
GATTTATCTGGGGAATATCTGAATGCCAAATTCGTTCTCTCTGAGATCGGAAGAGCACACG1320.14060952096892743No Hit
AAGATGCCGTCTGTCCGTACCAGGACGAGAAGACCCCATGGAGCTTTACTACAAGATCGGA1290.13741385003781545No Hit
AGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGGGG1270.13528340275040746TruSeq Adapter, Index 1 (97% over 36bp)
GTTACATCCTTCCCATAAGCCATCGAGCCCATACCCCGAAAATGCTGGTTACATCCTTCCC1240.13208773181929548No Hit
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGG1220.12995728453188748TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTAAGATCTGGGGGGGGGGGGGGG1090.11610937716373554TruSeq Adapter, Index 1 (97% over 36bp)
TAATGACCAACATCCGAAAATCACACCCACTAATAAAAATTATCAACAACGCATTCATTGA1080.11504415352003154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)