##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-EC4.merged.rmdup.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2277
Sequences flagged as poor quality	0
Sequence length	31-91
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.76328502415459	40.0	40.0	40.0	40.0	40.0
2	38.6772068511199	40.0	40.0	40.0	40.0	40.0
3	38.759771629336846	40.0	40.0	40.0	40.0	40.0
4	38.524374176548086	40.0	40.0	40.0	40.0	40.0
5	38.80193236714976	40.0	40.0	40.0	40.0	40.0
6	38.62450592885376	40.0	40.0	40.0	40.0	40.0
7	38.80017566974089	40.0	40.0	40.0	40.0	40.0
8	39.00219587176109	40.0	40.0	40.0	40.0	40.0
9	39.130434782608695	40.0	40.0	40.0	40.0	40.0
10-11	39.0540184453228	40.0	40.0	40.0	40.0	40.0
12-13	39.02239789196311	40.0	40.0	40.0	40.0	40.0
14-15	39.086517347386916	40.0	40.0	40.0	40.0	40.0
16-17	39.18577075098814	40.0	40.0	40.0	40.0	40.0
18-19	39.20333772507686	40.0	40.0	40.0	40.0	40.0
20-21	38.942468159859466	40.0	40.0	40.0	40.0	40.0
22-23	38.89855072463768	40.0	40.0	40.0	40.0	40.0
24-25	39.044356609574	40.0	40.0	40.0	40.0	40.0
26-27	38.62011418533157	40.0	40.0	40.0	40.0	40.0
28-29	38.52173913043478	40.0	40.0	40.0	40.0	40.0
30-31	38.81335090030742	40.0	40.0	40.0	40.0	40.0
32-33	38.986121473406754	40.0	40.0	40.0	40.0	40.0
34-35	39.02234448333778	40.0	40.0	40.0	40.0	40.0
36-37	39.085075448164545	40.0	40.0	40.0	40.0	40.0
38-39	39.04216881458261	40.0	40.0	40.0	40.0	40.0
40-41	38.97151084939905	40.0	40.0	40.0	40.0	40.0
42-43	39.13961108723679	40.0	40.0	40.0	40.0	40.0
44-45	39.052361024570146	40.0	40.0	40.0	40.0	40.0
46-47	39.2198824090259	40.0	40.0	40.0	40.0	40.0
48-49	39.07757061231048	40.0	40.0	40.0	40.0	40.0
50-51	38.98984673961118	40.0	40.0	40.0	40.0	40.0
52-53	38.823243754270685	40.0	40.0	40.0	40.0	40.0
54-55	38.93292767561711	40.0	40.0	40.0	40.0	40.0
56-57	39.00590646433976	40.0	40.0	40.0	40.0	40.0
58-59	39.02826120437394	40.0	40.0	40.0	40.0	40.0
60-61	38.95294627070328	40.0	40.0	40.0	40.0	40.0
62-63	39.00138962581167	40.0	40.0	40.0	40.0	40.0
64-65	38.86007609089778	40.0	40.0	40.0	40.0	40.0
66-67	39.28928571428571	40.0	40.0	40.0	40.0	40.0
68-69	38.89905459448693	40.0	40.0	40.0	40.0	40.0
70-71	38.71661676646707	40.0	40.0	40.0	40.0	40.0
72-73	38.97057196592543	40.0	40.0	40.0	40.0	40.0
74-75	38.750090546903294	40.0	40.0	40.0	40.0	40.0
76-77	38.807017543859644	40.0	40.0	40.0	40.0	40.0
78-79	39.356255054059716	40.0	40.0	40.0	40.0	40.0
80-81	38.43773584905661	40.0	40.0	40.0	40.0	40.0
82-83	38.76144018583042	40.0	40.0	40.0	40.0	40.0
84-85	39.28817092235539	40.0	40.0	40.0	40.0	40.0
86-87	38.43595505617978	NaN	NaN	NaN	NaN	NaN
88-89	39.09362706530291	NaN	NaN	NaN	NaN	NaN
90-91	36.578947368421055	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3081709956709986
1101	2	1.2932043650793688
1101	3	1.479556727994236
1101	4	1.3621031746031775
1101	5	1.078111471861483
1101	6	-2.4073795995671006
1101	7	1.0272997835497861
1101	8	1.3436688311688343
1101	9	1.025892857142857
1101	10-11	1.1962481962482059
1101	12-13	-0.9300696699134221
1101	14-15	1.5933159722222285
1101	16-17	1.1651718073593074
1101	18-19	-0.992194264069262
1101	20-21	-1.1002886002886
1101	22-23	0.7763483044733093
1101	24-25	0.8550065386002856
1101	26-27	1.085838293650795
1101	28-29	1.1767451298701275
1101	30-31	0.9506482233044764
1101	32-33	0.5239087301587304
1101	34-35	0.5626893939393938
1101	36-37	0.5154356060606062
1101	38-39	1.195328282828278
1101	40-41	1.1048611111111128
1101	42-43	0.9343750000000028
1101	44-45	0.6916666666666629
1101	46-47	0.7427083333333258
1101	48-49	0.5145833333333272
1101	50-51	1.1458333333333286
1101	52-53	1.124999999999993
1101	54-55	0.7916666666666643
1101	56-57	1.8104166666666615
1101	58-59	-2.0854166666666742
1101	60-61	6.53125
1101	62-63	-6.302083333333329
1101	64-65	0.125
1101	66-67	-1.5
1101	68-69	0.03125
1101	70-71	1.1770833333333357
1101	72-73	-4.625
1101	74-75	1.0625
1101	76-77	-8.0
1101	78-79	-16.8125
1101	80-81	-13.875
1101	82-83	-13.5
1101	84-85	-10.8125
1101	86-87	-8.0625
1101	88-89	-3.75
1102	1	1.3081709956709986
1102	2	1.2932043650793688
1102	3	1.479556727994236
1102	4	1.3621031746031775
1102	5	1.078111471861483
1102	6	1.5926204004328994
1102	7	1.0272997835497861
1102	8	1.3436688311688343
1102	9	1.025892857142857
1102	10-11	-2.803751803751794
1102	12-13	1.069930330086578
1102	14-15	1.5933159722222285
1102	16-17	1.1651718073593074
1102	18-19	-0.992194264069262
1102	20-21	-1.1002886002886
1102	22-23	0.7763483044733093
1102	24-25	0.8550065386002856
1102	26-27	1.085838293650795
1102	28-29	1.1767451298701275
1102	30-31	0.9506482233044764
1102	32-33	0.5239087301587304
1102	34-35	0.5626893939393938
1102	36-37	-2.151231060606065
1102	38-39	1.195328282828278
1102	40-41	1.1048611111111128
1102	42-43	0.9343750000000028
1102	44-45	0.6916666666666629
1102	46-47	0.7427083333333258
1102	48-49	0.5145833333333272
1102	50-51	1.1458333333333286
1102	52-53	-2.875000000000007
1102	54-55	0.7916666666666643
1102	56-57	1.8104166666666615
1102	58-59	1.9145833333333258
1102	60-61	6.53125
1102	62-63	7.697916666666671
1102	64-65	3.125
1102	66-67	4.5
1102	68-69	14.03125
1102	70-71	7.177083333333336
1102	72-73	3.375
1102	74-75	3.0625
1102	76-77	6.0
1102	78-79	23.1875
1102	80-81	12.125
1102	82-83	26.5
1102	84-85	-10.8125
1102	86-87	-8.0625
1102	88-89	-3.75
1103	1	1.3081709956709986
1103	2	1.2932043650793688
1103	3	1.479556727994236
1103	4	1.3621031746031775
1103	5	1.078111471861483
1103	6	1.5926204004328994
1103	7	-2.972700216450214
1103	8	1.3436688311688343
1103	9	1.025892857142857
1103	10-11	1.1962481962482059
1103	12-13	-2.430069669913422
1103	14-15	1.5933159722222285
1103	16-17	-2.8348281926406926
1103	18-19	1.007805735930738
1103	20-21	-1.1002886002886
1103	22-23	0.7763483044733093
1103	24-25	-3.1449934613997144
1103	26-27	-2.414161706349205
1103	28-29	1.1767451298701275
1103	30-31	0.9506482233044764
1103	32-33	-1.4760912698412696
1103	34-35	-2.1039772727272776
1103	36-37	0.5154356060606062
1103	38-39	-1.4713383838383933
1103	40-41	-6.228472222222223
1103	42-43	0.9343750000000028
1103	44-45	-1.9750000000000085
1103	46-47	-3.9239583333333385
1103	48-49	-4.152083333333337
1103	50-51	-3.5208333333333357
1103	52-53	-1.5416666666666785
1103	54-55	-7.875
1103	56-57	-9.189583333333339
1103	58-59	1.9145833333333258
1103	60-61	-14.46875
1103	62-63	-20.30208333333333
1103	64-65	-10.875
1103	66-67	-15.5
1103	68-69	-7.96875
1103	70-71	7.177083333333336
1103	72-73	-22.625
1103	74-75	-22.9375
1103	76-77	-20.0
1103	78-79	-16.8125
1103	80-81	-13.875
1103	82-83	-13.5
1103	84-85	-10.8125
1103	86-87	-8.0625
1103	88-89	-3.75
1104	1	1.3081709956709986
1104	2	1.2932043650793688
1104	3	1.479556727994236
1104	4	1.3621031746031775
1104	5	1.078111471861483
1104	6	1.5926204004328994
1104	7	1.0272997835497861
1104	8	-2.6563311688311657
1104	9	1.025892857142857
1104	10-11	1.1962481962482059
1104	12-13	1.069930330086578
1104	14-15	1.5933159722222285
1104	16-17	1.1651718073593074
1104	18-19	1.007805735930738
1104	20-21	0.8997113997113999
1104	22-23	0.7763483044733093
1104	24-25	0.8550065386002856
1104	26-27	1.085838293650795
1104	28-29	1.1767451298701275
1104	30-31	0.9506482233044764
1104	32-33	0.5239087301587304
1104	34-35	0.5626893939393938
1104	36-37	0.5154356060606062
1104	38-39	1.195328282828278
1104	40-41	1.1048611111111128
1104	42-43	0.9343750000000028
1104	44-45	0.6916666666666629
1104	46-47	0.7427083333333258
1104	48-49	0.5145833333333272
1104	50-51	1.1458333333333286
1104	52-53	1.124999999999993
1104	54-55	0.7916666666666643
1104	56-57	1.8104166666666615
1104	58-59	1.9145833333333258
1104	60-61	6.53125
1104	62-63	7.697916666666671
1104	64-65	11.125
1104	66-67	12.5
1104	68-69	14.03125
1104	70-71	15.177083333333336
1104	72-73	17.375
1104	74-75	17.0625
1104	76-77	-20.0
1104	78-79	-16.8125
1104	80-81	-13.875
1104	82-83	-13.5
1104	84-85	-10.8125
1104	86-87	-8.0625
1104	88-89	-3.75
1201	1	-8.025162337662334
1201	2	-4.040128968253967
1201	3	1.479556727994236
1201	4	1.3621031746031775
1201	5	1.078111471861483
1201	6	1.5926204004328994
1201	7	1.0272997835497861
1201	8	1.3436688311688343
1201	9	1.025892857142857
1201	10-11	1.1962481962482059
1201	12-13	1.069930330086578
1201	14-15	1.5933159722222285
1201	16-17	1.1651718073593074
1201	18-19	-1.6588609307359334
1201	20-21	-1.7669552669552715
1201	22-23	0.7763483044733093
1201	24-25	-1.8116601280663858
1201	26-27	1.085838293650795
1201	28-29	1.1767451298701275
1201	30-31	0.9506482233044764
1201	32-33	0.5239087301587304
1201	34-35	0.5626893939393938
1201	36-37	0.5154356060606062
1201	38-39	1.195328282828278
1201	40-41	1.1048611111111128
1201	42-43	-7.065624999999997
1201	44-45	0.6916666666666629
1201	46-47	0.7427083333333258
1201	48-49	0.5145833333333272
1201	50-51	1.1458333333333286
1201	52-53	1.124999999999993
1201	54-55	0.7916666666666643
1201	56-57	1.8104166666666615
1201	58-59	1.9145833333333258
1201	60-61	-33.46875
1201	62-63	-32.30208333333333
1201	64-65	-28.875
1201	66-67	-27.5
1201	68-69	-25.96875
1201	70-71	-24.822916666666664
1201	72-73	-22.625
1201	74-75	-22.9375
1201	76-77	-20.0
1201	78-79	-16.8125
1201	80-81	-13.875
1201	82-83	-13.5
1201	84-85	-10.8125
1201	86-87	-8.0625
1201	88-89	-3.75
1202	1	-1.3584956709956657
1202	2	-3.3734623015872955
1202	3	-3.8537766053390996
1202	4	-1.3045634920634868
1202	5	1.078111471861483
1202	6	-3.7407129329004363
1202	7	-1.6393668831168782
1202	8	1.3436688311688343
1202	9	-1.6407738095238074
1202	10-11	1.1962481962482059
1202	12-13	-0.2634030032467507
1202	14-15	1.5933159722222285
1202	16-17	-2.501494859307357
1202	18-19	1.007805735930738
1202	20-21	0.8997113997113999
1202	22-23	-0.5569850288600193
1202	24-25	-1.478326794733043
1202	26-27	1.085838293650795
1202	28-29	-0.15658820346320113
1202	30-31	0.9506482233044764
1202	32-33	0.5239087301587304
1202	34-35	0.5626893939393938
1202	36-37	-2.9845643939393938
1202	38-39	-0.8046717171717219
1202	40-41	-2.395138888888887
1202	42-43	-5.232291666666669
1202	44-45	0.6916666666666629
1202	46-47	0.7427083333333258
1202	48-49	-2.1520833333333442
1202	50-51	1.1458333333333286
1202	52-53	-1.5416666666666785
1202	54-55	-3.2083333333333357
1202	56-57	1.8104166666666615
1202	58-59	-5.085416666666674
1202	60-61	2.53125
1202	62-63	0.6979166666666714
1202	64-65	11.125
1202	66-67	12.5
1202	68-69	14.03125
1202	70-71	15.177083333333336
1202	72-73	17.375
1202	74-75	17.0625
1202	76-77	20.0
1202	78-79	23.1875
1202	80-81	26.125
1202	82-83	26.5
1202	84-85	29.1875
1202	86-87	-8.0625
1202	88-89	-3.75
1203	1	1.3081709956709986
1203	2	1.2932043650793688
1203	3	1.479556727994236
1203	4	1.3621031746031775
1203	5	1.078111471861483
1203	6	1.5926204004328994
1203	7	1.0272997835497861
1203	8	1.3436688311688343
1203	9	1.025892857142857
1203	10-11	1.1962481962482059
1203	12-13	1.069930330086578
1203	14-15	-3.740017361111107
1203	16-17	1.1651718073593074
1203	18-19	-6.325527597402598
1203	20-21	0.8997113997113999
1203	22-23	-1.8903183621933621
1203	24-25	0.8550065386002856
1203	26-27	1.085838293650795
1203	28-29	1.1767451298701275
1203	30-31	0.9506482233044764
1203	32-33	0.5239087301587304
1203	34-35	0.5626893939393938
1203	36-37	0.5154356060606062
1203	38-39	1.195328282828278
1203	40-41	1.1048611111111128
1203	42-43	0.9343750000000028
1203	44-45	0.6916666666666629
1203	46-47	0.7427083333333258
1203	48-49	0.5145833333333272
1203	50-51	-2.8541666666666714
1203	52-53	1.124999999999993
1203	54-55	0.7916666666666643
1203	56-57	1.8104166666666615
1203	58-59	1.9145833333333258
1203	60-61	6.53125
1203	62-63	7.697916666666671
1203	64-65	-28.875
1203	66-67	-27.5
1203	68-69	-25.96875
1203	70-71	-24.822916666666664
1203	72-73	-22.625
1203	74-75	-22.9375
1203	76-77	-20.0
1203	78-79	-16.8125
1203	80-81	-13.875
1203	82-83	-13.5
1203	84-85	-10.8125
1203	86-87	-8.0625
1203	88-89	-3.75
1204	1	1.3081709956709986
1204	2	1.2932043650793688
1204	3	-4.9204432720057625
1204	4	-1.8378968253968253
1204	5	-5.3218885281385155
1204	6	-4.807379599567099
1204	7	-5.3727002164502125
1204	8	-1.8563311688311686
1204	9	-2.174107142857146
1204	10-11	-0.40375180375179553
1204	12-13	-2.130069669913425
1204	14-15	-4.006684027777773
1204	16-17	-0.43482819264069406
1204	18-19	1.007805735930738
1204	20-21	-0.7002886002886015
1204	22-23	-0.8236516955266922
1204	24-25	-2.344993461399717
1204	26-27	1.085838293650795
1204	28-29	-2.0232548701298754
1204	30-31	0.9506482233044764
1204	32-33	0.5239087301587304
1204	34-35	0.5626893939393938
1204	36-37	0.5154356060606062
1204	38-39	1.195328282828278
1204	40-41	-1.5618055555555586
1204	42-43	0.9343750000000028
1204	44-45	-1.9750000000000085
1204	46-47	0.7427083333333258
1204	48-49	0.5145833333333272
1204	50-51	1.1458333333333286
1204	52-53	1.124999999999993
1204	54-55	0.7916666666666643
1204	56-57	1.8104166666666615
1204	58-59	1.9145833333333258
1204	60-61	6.53125
1204	62-63	-0.3020833333333286
1204	64-65	3.125
1204	66-67	-1.5
1204	68-69	-7.96875
1204	70-71	-6.822916666666664
1204	72-73	9.375
1204	74-75	-4.9375
1204	76-77	6.0
1204	78-79	-16.8125
1204	80-81	-13.875
1204	82-83	-13.5
1204	84-85	-10.8125
1204	86-87	-8.0625
1204	88-89	-3.75
1301	1	1.3081709956709986
1301	2	1.2932043650793688
1301	3	-3.8537766053390996
1301	4	1.3621031746031775
1301	5	1.078111471861483
1301	6	-3.7407129329004363
1301	7	1.0272997835497861
1301	8	-3.9896645021645014
1301	9	-4.307440476190479
1301	10-11	-3.4704184704184584
1301	12-13	1.069930330086578
1301	14-15	-3.0733506944444358
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2401	86-87	-8.0625
2401	88-89	-3.75
2402	1	-0.14637445887445466
2402	2	1.2932043650793688
2402	3	1.479556727994236
2402	4	1.3621031746031775
2402	5	1.078111471861483
2402	6	1.5926204004328994
2402	7	1.0272997835497861
2402	8	1.3436688311688343
2402	9	1.025892857142857
2402	10-11	-0.25829725829724737
2402	12-13	-0.38461512445887536
2402	14-15	0.8660432449495019
2402	16-17	1.1651718073593074
2402	18-19	1.007805735930738
2402	20-21	0.8997113997113999
2402	22-23	0.7763483044733093
2402	24-25	0.8550065386002856
2402	26-27	1.085838293650795
2402	28-29	1.1767451298701275
2402	30-31	0.9506482233044764
2402	32-33	0.5239087301587304
2402	34-35	0.5626893939393938
2402	36-37	0.5154356060606062
2402	38-39	-0.5824494949495005
2402	40-41	0.21597222222222712
2402	42-43	0.9343750000000028
2402	44-45	0.6916666666666629
2402	46-47	0.7427083333333258
2402	48-49	-0.4854166666666728
2402	50-51	1.1458333333333286
2402	52-53	1.124999999999993
2402	54-55	0.7916666666666643
2402	56-57	1.8104166666666615
2402	58-59	0.31458333333332433
2402	60-61	6.53125
2402	62-63	7.697916666666671
2402	64-65	11.125
2402	66-67	12.5
2402	68-69	14.03125
2402	70-71	15.177083333333336
2402	72-73	9.375
2402	74-75	17.0625
2402	76-77	12.0
2402	78-79	23.1875
2402	80-81	26.125
2402	82-83	26.5
2402	84-85	29.1875
2402	86-87	31.9375
2402	88-89	-3.75
2403	1	0.3081709956709986
2403	2	-1.7067956349206312
2403	3	-0.2704432720057639
2403	4	-0.6378968253968225
2403	5	0.07811147186148304
2403	6	-0.15737959956710057
2403	7	0.027299783549786127
2403	8	1.3436688311688343
2403	9	0.02589285714285694
2403	10-11	0.1962481962482059
2403	12-13	-1.305069669913422
2403	14-15	-0.28168402777777146
2403	16-17	0.6651718073593074
2403	18-19	0.507805735930738
2403	20-21	-0.1002886002886001
2403	22-23	-0.7236516955266907
2403	24-25	-0.5199934613997144
2403	26-27	-0.4141617063492049
2403	28-29	0.6767451298701275
2403	30-31	-0.4243517766955236
2403	32-33	0.5239087301587304
2403	34-35	0.5626893939393938
2403	36-37	-0.2118371212121204
2403	38-39	-0.0773989898989953
2403	40-41	0.3048611111111157
2403	42-43	0.9343750000000028
2403	44-45	0.6916666666666629
2403	46-47	0.7427083333333258
2403	48-49	0.5145833333333272
2403	50-51	-1.5208333333333357
2403	52-53	-0.2083333333333357
2403	54-55	0.7916666666666643
2403	56-57	0.21041666666666003
2403	58-59	1.9145833333333258
2403	60-61	4.53125
2403	62-63	0.3645833333333357
2403	64-65	0.125
2403	66-67	8.5
2403	68-69	3.03125
2403	70-71	7.177083333333336
2403	72-73	9.375
2403	74-75	17.0625
2403	76-77	20.0
2403	78-79	-16.8125
2403	80-81	-13.875
2403	82-83	-13.5
2403	84-85	-10.8125
2403	86-87	-8.0625
2403	88-89	-3.75
2404	1	-0.46960678210678
2404	2	1.2932043650793688
2404	3	1.479556727994236
2404	4	0.4732142857142918
2404	5	1.078111471861483
2404	6	0.7037315115440137
2404	7	1.0272997835497861
2404	8	0.45477994227994856
2404	9	1.025892857142857
2404	10-11	1.1962481962482059
2404	12-13	1.069930330086578
2404	14-15	1.5933159722222285
2404	16-17	0.7207273629148645
2404	18-19	0.5633612914862951
2404	20-21	-0.4336219336219287
2404	22-23	-0.11254058441557646
2404	24-25	0.41056209415584277
2404	26-27	0.6413938492063522
2404	28-29	-0.04547709235209396
2404	30-31	0.5062037788600335
2404	32-33	0.5239087301587304
2404	34-35	-0.7100378787878796
2404	36-37	0.5154356060606062
2404	38-39	1.195328282828278
2404	40-41	1.1048611111111128
2404	42-43	0.9343750000000028
2404	44-45	0.6916666666666629
2404	46-47	0.7427083333333258
2404	48-49	0.5145833333333272
2404	50-51	1.1458333333333286
2404	52-53	1.124999999999993
2404	54-55	0.7916666666666643
2404	56-57	1.8104166666666615
2404	58-59	1.9145833333333258
2404	60-61	6.53125
2404	62-63	7.697916666666671
2404	64-65	-28.875
2404	66-67	-27.5
2404	68-69	-25.96875
2404	70-71	-24.822916666666664
2404	72-73	-22.625
2404	74-75	-22.9375
2404	76-77	-20.0
2404	78-79	-16.8125
2404	80-81	-13.875
2404	82-83	-13.5
2404	84-85	-10.8125
2404	86-87	-8.0625
2404	88-89	-3.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
31	5.0
32	26.0
33	39.0
34	56.0
35	80.0
36	94.0
37	108.0
38	228.0
39	786.0
40	855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.806763285024154	22.968818620992533	32.54281949934124	24.681598594642075
2	20.948616600790515	22.837066315327185	30.566534914361	25.6477821695213
3	21.783047870004392	23.232323232323232	31.44488361879666	23.539745278875714
4	20.59727711901625	23.49582784365393	27.84365393061045	28.063241106719367
5	23.10057092665788	21.73913043478261	30.566534914361	24.593763724198507
6	22.573561703996486	22.74923144488362	27.09705753184014	27.580149319279755
7	22.090469916556874	23.10057092665788	29.20509442248573	25.60386473429952
8	18.48924022837066	44.883618796662276	14.624505928853754	22.002635046113305
9	41.80939833113746	19.894598155467722	20.860781730346947	17.43522178304787
10-11	18.73078612209047	28.634167764602548	32.54281949934124	20.092226613965742
12-13	31.68642951251647	16.007905138339922	18.028107158541943	34.27755819060167
14-15	30.21519543258674	32.8941589811155	15.98594642072903	20.90469916556873
16-17	29.97364953886693	30.698287220026348	17.14975845410628	22.17830478700044
18-19	30.303030303030305	23.737373737373737	19.521299956082565	26.438296003513393
20-21	29.885814668423365	14.295125164690381	23.144488361879667	32.674571805006586
22-23	26.306543697848046	16.425120772946862	27.77777777777778	29.490557751427314
24-25	31.620553359683797	18.533157663592444	30.171277997364953	19.675010979358806
26-27	30.588493631971893	25.71365832235398	24.967061923583664	18.73078612209047
28-29	37.417654808959156	26.438296003513393	18.1379007465964	18.00614844093105
30-31	28.985507246376812	37.17610891523935	16.97408871321915	16.86429512516469
32-33	27.056962025316455	33.64978902953587	19.435654008438817	19.85759493670886
34-35	27.728321793603694	24.56313880646225	24.92581602373887	22.782723376195186
36-37	28.3704572098476	25.43962485345838	22.00078155529504	24.189136381398985
38-39	26.107325383304943	25.340715502555366	20.61328790459966	27.938671209540033
40-41	27.076784912438256	23.30489447687472	22.272114952851368	27.346205657835654
42-43	27.010406811731315	25.402081362346262	24.17218543046358	23.415326395458848
44-45	25.384615384615383	23.435897435897434	22.82051282051282	28.358974358974358
46-47	25.53551296505073	24.182638105975197	22.71702367531003	27.56482525366404
48-49	27.065868263473053	22.994011976047904	22.395209580838323	27.54491017964072
50-51	23.354430379746834	23.544303797468356	28.354430379746837	24.746835443037973
52-53	24.555403556771545	21.3406292749658	22.503419972640216	31.600547195622436
54-55	24.00897531787584	28.421839940164546	23.036649214659686	24.532535527299927
56-57	25.919999999999998	23.200000000000003	23.68	27.200000000000003
58-59	27.68701633705933	26.225279449699052	20.46431642304385	25.623387790197764
60-61	24.501424501424502	21.367521367521366	26.21082621082621	27.92022792022792
62-63	22.46153846153846	30.153846153846153	24.41025641025641	22.974358974358974
64-65	25.813953488372093	25.465116279069765	24.651162790697676	24.069767441860463
66-67	21.656050955414013	24.076433121019107	26.24203821656051	28.02547770700637
68-69	23.09820193637621	24.343015214384508	22.959889349930844	29.59889349930844
70-71	26.911314984709477	18.34862385321101	27.981651376146786	26.758409785932724
72-73	25.8364312267658	20.817843866171003	27.137546468401485	26.20817843866171
74-75	24.137931034482758	22.718052738336713	26.16632860040568	26.97768762677485
76-77	28.99543378995434	24.885844748858446	20.091324200913242	26.027397260273972
78-79	21.584699453551913	26.775956284153008	30.87431693989071	20.76502732240437
80-81	22.839506172839506	19.753086419753085	26.851851851851855	30.555555555555557
82-83	32.31939163498099	30.798479087452474	15.96958174904943	20.912547528517113
84-85	25.116279069767444	22.790697674418606	25.581395348837212	26.51162790697674
86-87	14.02439024390244	40.853658536585364	13.414634146341465	31.70731707317073
88-89	26.38888888888889	30.555555555555557	25.0	18.055555555555554
90-91	24.137931034482758	20.689655172413794	37.93103448275862	17.24137931034483
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.5
17	3.5
18	3.0
19	2.0
20	1.0
21	0.5
22	1.0
23	1.0
24	2.0
25	3.5
26	4.0
27	7.0
28	9.5
29	9.5
30	13.5
31	16.0
32	13.0
33	11.5
34	16.5
35	17.0
36	17.0
37	28.0
38	36.0
39	49.5
40	73.5
41	111.5
42	148.0
43	173.0
44	226.0
45	280.5
46	309.5
47	328.5
48	325.0
49	318.5
50	316.5
51	294.0
52	255.5
53	212.5
54	182.0
55	169.5
56	148.5
57	116.0
58	97.0
59	94.0
60	96.5
61	94.5
62	85.0
63	71.0
64	58.5
65	63.5
66	68.0
67	58.5
68	49.5
69	41.5
70	40.5
71	36.0
72	30.0
73	29.5
74	26.0
75	19.5
76	16.0
77	15.0
78	11.5
79	8.5
80	5.0
81	3.0
82	2.0
83	1.5
84	2.0
85	2.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-31	265.0
32-33	416.0
34-35	277.0
36-37	131.0
38-39	61.0
40-41	53.0
42-43	76.0
44-45	99.0
46-47	52.0
48-49	49.0
50-51	53.0
52-53	62.0
54-55	52.0
56-57	27.0
58-59	69.0
60-61	34.0
62-63	60.0
64-65	41.0
66-67	26.0
68-69	40.0
70-71	58.0
72-73	25.0
74-75	23.0
76-77	35.0
78-79	28.0
80-81	25.0
82-83	26.0
84-85	25.0
86-87	48.0
88-89	22.0
90-91	19.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.63855950812473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.3766696349065	98.02371541501977
2	0.3561887800534283	0.7026789635485288
3	0.08904719501335707	0.2635046113306983
4	0.08904719501335707	0.3513394817742644
5	0.04452359750667854	0.21958717610891526
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.04452359750667854	0.4391743522178305
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAATTCTGTGACATATCAGGGCGGCCAATTAGTAAATTGTCTTGGACCAG	10	0.4391743522178305	No Hit
CCTTCCTATCTTAGGAGAAGAAACATTGTCTTCATGAACCGGATTTGGCT	5	0.21958717610891526	No Hit
CCATATGTTTACCGTAGGAATAGATGTAGACACACGAGCGTACTTTACGT	4	0.1756697408871322	No Hit
ATTCAACAGGAACAGGAAGAATCTCGTATTTACTCTTGGGACGACAATAA	4	0.1756697408871322	No Hit
CGGTGGCTTGTGGATGCGCCGTCACTCAACGGATAAAAGGTACCCCGGGG	3	0.13175230566534915	No Hit
TTGAAACCACTCATTTGTTCCCCCTAAATAAGACATCTCAGTGACTAATG	3	0.13175230566534915	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.04391743522178305	0.0	0.0	0.0	0.0
4	0.1756697408871322	0.0	0.0	0.0	0.0
5	0.5270092226613966	0.0	0.0	0.0	0.0
6	1.1418533157663593	0.0	0.0	0.0	0.0
7	2.1519543258673695	0.0	0.0	0.0	0.0
8	15.458937198067632	0.0	0.0	0.0	0.0
9	15.985946420729029	0.0	0.0	0.0	0.0
10-11	16.095740008783487	0.0	0.0	0.0	0.0
12-13	16.11769872639438	0.0	0.0	0.0	0.0
14-15	16.11769872639438	0.0	0.0	0.0	0.0
16-17	16.11769872639438	0.0	0.0	0.0	0.0
18-19	16.11769872639438	0.0	0.0	0.0	0.0
20-21	16.11769872639438	0.0	0.0	0.0	0.0
22-23	16.11769872639438	0.0	0.0	0.0	0.0
24-25	16.11769872639438	0.0	0.0	0.0	0.0
26-27	16.11769872639438	0.0	0.0	0.0	0.0
28-29	16.22749231444884	0.0	0.0	0.0	0.0
30-31	16.337285902503293	0.0	0.0	0.0	0.0
32-33	16.40316205533597	0.0	0.0	0.0	0.0
34-35	16.55687307861221	0.0	0.0	0.0	0.0
36-37	16.644707949055775	0.0	0.0	0.0	0.0
38-39	16.798418972332016	0.0	0.0	0.0	0.0
40-41	16.908212560386474	0.0	0.0	0.0	0.0
42-43	16.974088713219146	0.0	0.0	0.0	0.0
44-45	17.03996486605182	0.0	0.0	0.0	0.0
46-47	17.03996486605182	0.0	0.0	0.0	0.0
48-49	17.03996486605182	0.0	0.0	0.0	0.0
50-51	17.03996486605182	0.0	0.0	0.0	0.0
52-53	17.03996486605182	0.0	0.0	0.0	0.0
54-55	17.03996486605182	0.0	0.0	0.0	0.0
56-57	17.03996486605182	0.0	0.0	0.0	0.0
58-59	17.03996486605182	0.0	0.0	0.0	0.0
60-61	17.03996486605182	0.0	0.0	0.0	0.0
62-63	17.03996486605182	0.0	0.0	0.0	0.0
64-65	17.03996486605182	0.0	0.0	0.0	0.0
66-67	17.03996486605182	0.0	0.0	0.0	0.0
68-69	17.03996486605182	0.0	0.0	0.0	0.0
70-71	17.03996486605182	0.0	0.0	0.0	0.0
72-73	17.03996486605182	0.0	0.0	0.0	0.0
74-75	17.03996486605182	0.0	0.0	0.0	0.0
76-77	17.03996486605182	0.0	0.0	0.0	0.0
78-79	17.03996486605182	0.0	0.0	0.0	0.0
80	17.03996486605182	0.0	0.0	0.0	0.0
>>END_MODULE