AM-AL-EC3_S25_R2_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	AM-AL-EC3_S25_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65318
Sequences flagged as poor quality	0
Sequence length	61
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCCCCCTTGTCTC	8613	13.186257999326372	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCCCCCTTGTCCC	2724	4.170366514590159	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCGACCCCCCTTGTCTC	2655	4.064729477326312	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCCTTTCCACCCCCCTTGTCTC	2153	3.296181756942956	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCTTTTCCACCCCCCTTGTCTC	2134	3.267093297406534	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCCACCCCCCTTGTCTC	2120	3.245659695642855	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCCCCGTTGTCTC	1342	2.054563826204109	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCCCCCTTTTCTC	1314	2.0116966226767508	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCCCCCTTTTCCC	1235	1.8907498698674179	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCGACCCCCGTTGTCTC	1009	1.5447502985394532	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCATTTCCACCCCCCTTGTCTC	751	1.149759637465936	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCCTTTCCACCCCCCTTGTCTC	678	1.0379987139838942	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCGACCCCCCTTGTCTC	654	1.0012553966747297	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCCACCCCCCTTGTCCC	610	0.933892648274595	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCTTTTCCACCCCCCTTGTCCC	594	0.9093971034018189	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCTCCCTTGTCTC	571	0.8741847576472028	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCCACCCCCGTTGTCTC	567	0.8680608714290088	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCTTTTCGACCCCCCTTGTCTC	554	0.8481582412198782	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCTTTTCCACCCCCCTTTTCTC	470	0.7195566306378027	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCCTTTCCACCCCCCTTGTCCC	445	0.6812823417740899	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCCTTTCGACCCCCCTTGTCTC	443	0.6782203986649928	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCGACCCCCGTTGTCTC	404	0.6185125080376007	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCCTTTCCACCCCCCTTTTCTC	390	0.5970789062739215	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCTTTTCCACCCCCCTTTTCCC	360	0.5511497596374659	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCGTTTCCACCCCCCTTGTCTC	348	0.5327781009828838	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCCCCGTTGTCCC	344	0.5266542147646897	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCGACCTCCCTTGTCTC	341	0.5220613001010441	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCCCTTTTCCACCCCCCTTGTCTC	314	0.48072506812823423	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCGACCTCCGTTGTCTC	246	0.37661900241893503	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCTTTTCCACCCCCGTTGTCTC	239	0.36590220153709546	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCGACCCCCCTTGTCCC	236	0.3613092868734499	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCATCCCCCTTGTCTC	218	0.3337517988915766	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCCCCTTTCCACCCCCCTTGTCTC	213	0.326096941118834	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCCTTTCCACCCCCCTTTTCTC	191	0.29241556691876663	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCCACCCCCCTTTTCTC	183	0.2801677944823785	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCATTTCCACCCCCCTTGTCCC	174	0.2663890504914419	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCCTTTCCACCCCCCTTTTCCC	172	0.2633271073823448	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCCACCCCCCTTTTCCC	168	0.2572032211641508	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCCTTTCGACCCCCCTTGTCTC	167	0.2556722496096022	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCTCCGTTGTCTC	164	0.2510793349459567	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCATTTCGACCCCCCTTGTCTC	163	0.2495483633914082	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCCTTTCCACCCCCGTTGTCTC	156	0.23883156250956855	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCCCTTTTCCACCCCCCTTGTCTC	150	0.22964573318227746	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCCACCTCCCTTGTCTC	149	0.22811476162772895	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCATTTCCACCCCCGTTGTCTC	148	0.22658379007318044	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCTTTTCGACCCCCGTTGTCTC	148	0.22658379007318044	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCCCCCTGGTCTC	145	0.22199087540953488	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCGATCCCCCTTGTCTC	141	0.21586698919134084	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCTCCCTTGTCCC	141	0.21586698919134084	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTCCCACCTCTTTTCCACCCCCCTTGTCTC	139	0.21280504608224377	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCCTTTCCACCCCCCTTGTCCC	136	0.20821213141859826	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCCCGTTTCCACCCCCCTTGTCTC	134	0.20515018830950119	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCTTTTCCACCTCCCTTGTCTC	134	0.20515018830950119	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCAACTCTTTTCCACCCCCCTTGTCTC	131	0.20055727364585565	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCGACCCCCCTTTTCTC	127	0.19443338742766159	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCCCTTTTCCACCCCCCTTGTCCC	124	0.18984047276401603	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCGACCCCCCTGGTCTC	117	0.17912367188217643	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCATTTCCACCCCCCTTGTCTC	116	0.17759270032762792	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCCCCGTTTTCTC	107	0.16381395633669127	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCCACCCCCGTTGTCCC	106	0.16228298478214276	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCCCCTTTCCACCCCCCTTGTCTC	99	0.15156618390030313	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCTCCCTTTTCTC	99	0.15156618390030313	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCCCATTTCCACCCCCCTTGTCTC	97	0.14850424079120608	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTGTCCACCCCCCTTGTCTC	95	0.14544229768210906	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCGTTTCGACCCCCCTTGTCTC	95	0.14544229768210906	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCCTTTCCACCCCCCTTTTCCC	91	0.139318411463915	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCTTTTCCATCCCCCTTGTCTC	90	0.13778743990936648	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCCTTTCGACCCCCGTTGTCTC	89	0.13625646835481794	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCGACCCCCGTGGTCTC	88	0.13472549680026946	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCGATCCCCGTTGTCTC	87	0.13319452524572095	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCGACCCCCGTTGTCCC	87	0.13319452524572095	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCGACCTCCCTTGTCTC	83	0.12707063902752685	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCATTTCCACCCCCCTTTTCTC	79	0.1209467528093328	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCCCTTTTCGACCCCCCTTGTCTC	78	0.11941578125478428	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCACCCCCGTGGTCTC	78	0.11941578125478428	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCGACCCCCCTTGTCCC	75	0.11482286659113873	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCGTTTCGACCTCCGTTGTCTC	74	0.11329189503659022	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCCCCCCCCTTGTCTC	73	0.11176092348204171	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCACCTCTTTTCCATCCCCGTTGTCTC	72	0.11022995192749319	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTCCCACCTCTTTTCGACCCCCCTTGTCTC	72	0.11022995192749319	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCTCTTTTCGACCTCCCTTGTCTC	71	0.10869898037294468	Illumina Single End PCR Primer 1 (96% over 29bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content