##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-EC3_S25_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65318
Sequences flagged as poor quality	0
Sequence length	61
%GC	61
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.408616307909	40.0	40.0	40.0	40.0	40.0
2	39.11690498790532	40.0	40.0	40.0	40.0	40.0
3	38.98787470528798	40.0	40.0	40.0	40.0	40.0
4	39.47346826295968	40.0	40.0	40.0	40.0	40.0
5	39.51725404941976	40.0	40.0	40.0	40.0	40.0
6	39.100952264306926	40.0	40.0	40.0	40.0	40.0
7	39.26366392112435	40.0	40.0	40.0	40.0	40.0
8	39.1101993324964	40.0	40.0	40.0	40.0	40.0
9	39.320983496126644	40.0	40.0	40.0	40.0	40.0
10	39.37585351664166	40.0	40.0	40.0	40.0	40.0
11	39.44431856456107	40.0	40.0	40.0	40.0	40.0
12	39.480449493248415	40.0	40.0	40.0	40.0	40.0
13	39.595333598701735	40.0	40.0	40.0	40.0	40.0
14	39.44248139869561	40.0	40.0	40.0	40.0	40.0
15	39.79668697755596	40.0	40.0	40.0	40.0	40.0
16	39.571205487002054	40.0	40.0	40.0	40.0	40.0
17	39.74628739398022	40.0	40.0	40.0	40.0	40.0
18	39.478367371934226	40.0	40.0	40.0	40.0	40.0
19	39.71064637619033	40.0	40.0	40.0	40.0	40.0
20	38.114394194555864	40.0	40.0	40.0	24.0	40.0
21	39.668269083560425	40.0	40.0	40.0	40.0	40.0
22	39.526439878747055	40.0	40.0	40.0	40.0	40.0
23	39.57683946232279	40.0	40.0	40.0	40.0	40.0
24	39.543280565847084	40.0	40.0	40.0	40.0	40.0
25	39.883768639578676	40.0	40.0	40.0	40.0	40.0
26	39.75106402523041	40.0	40.0	40.0	40.0	40.0
27	39.75920879390061	40.0	40.0	40.0	40.0	40.0
28	39.7375302366882	40.0	40.0	40.0	40.0	40.0
29	39.56998070975841	40.0	40.0	40.0	40.0	40.0
30	39.61407269052941	40.0	40.0	40.0	40.0	40.0
31	39.74347040631985	40.0	40.0	40.0	40.0	40.0
32	39.851556998070976	40.0	40.0	40.0	40.0	40.0
33	39.61113322514468	40.0	40.0	40.0	40.0	40.0
34	39.80495422395052	40.0	40.0	40.0	40.0	40.0
35	39.823570838053826	40.0	40.0	40.0	40.0	40.0
36	39.559325147738754	40.0	40.0	40.0	40.0	40.0
37	39.51217122385866	40.0	40.0	40.0	40.0	40.0
38	39.571266725864234	40.0	40.0	40.0	40.0	40.0
39	38.42567133102667	40.0	40.0	40.0	40.0	40.0
40	39.58865856272391	40.0	40.0	40.0	40.0	40.0
41	39.58498423099299	40.0	40.0	40.0	40.0	40.0
42	38.26792002204599	40.0	40.0	40.0	40.0	40.0
43	39.363054594445636	40.0	40.0	40.0	40.0	40.0
44	39.379160415199486	40.0	40.0	40.0	40.0	40.0
45	34.78967512783613	40.0	24.0	40.0	24.0	40.0
46	37.52509262377905	40.0	40.0	40.0	24.0	40.0
47	34.207109831899324	40.0	24.0	40.0	24.0	40.0
48	38.69781683456321	40.0	40.0	40.0	40.0	40.0
49	38.86426406197373	40.0	40.0	40.0	40.0	40.0
50	38.670688018616616	40.0	40.0	40.0	40.0	40.0
51	38.13778743990937	40.0	40.0	40.0	40.0	40.0
52	38.0403564101779	40.0	40.0	40.0	40.0	40.0
53	36.955571205487004	40.0	40.0	40.0	24.0	40.0
54	35.76741480143299	40.0	40.0	40.0	24.0	40.0
55	35.793441317860314	40.0	40.0	40.0	24.0	40.0
56	35.56183594108821	40.0	40.0	40.0	24.0	40.0
57	33.91604151994856	40.0	24.0	40.0	24.0	40.0
58	30.220827337028076	24.0	24.0	40.0	12.0	40.0
59	25.602253590128296	24.0	24.0	40.0	12.0	40.0
60	24.617042775345233	24.0	12.0	24.0	12.0	40.0
61	22.0637496555314	24.0	12.0	24.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.06711190091220232
1101	2	0.10600296697413114
1101	3	0.4792407251055266
1101	4	0.10070970464121842
1101	5	0.2023204517731898
1101	6	0.11712642279615437
1101	7	0.011444155773219222
1101	8	0.5013978659386282
1101	9	-0.4399252408834542
1101	10	0.1872365347520173
1101	11	-0.12712725670858305
1101	12	0.19216352074295884
1101	13	0.20702643984805746
1101	14	0.022216296931460988
1101	15	0.1574743385979147
1101	16	0.4114702553514107
1101	17	0.15627968189002672
1101	18	0.41163479247469326
1101	19	0.23172072682483957
1101	20	0.5197667306025409
1101	21	0.18315887603223047
1101	22	0.24488973754517218
1101	23	0.010072681365450364
1101	24	0.16890041537359224
1101	25	-0.017363253349522267
1101	26	0.2224564746208486
1101	27	0.20199823716546206
1101	28	0.07004326706704944
1101	29	0.09555432029942068
1101	30	0.12266173184298879
1101	31	0.17519486684403773
1101	32	-0.0036144610471069427
1101	33	0.05840142780948554
1101	34	0.11602064811925317
1101	35	0.03168375865057982
1101	36	0.07811794908061387
1101	37	0.0960805496286028
1101	38	-0.0760388680793298
1101	39	1.3779107458385553
1101	40	0.2859444577687924
1101	41	0.23011135715073294
1101	42	-0.07605798982025647
1101	43	0.35180356471870056
1101	44	-0.058404582492805446
1101	45	1.0915889679885566
1101	46	-0.2907758289859288
1101	47	-1.174461074694591
1101	48	0.20112011893777293
1101	49	0.009153325170437654
1101	50	-0.10883877835514966
1101	51	-0.19689470403847054
1101	52	0.6338911245190957
1101	53	-0.35959312776346053
1101	54	-1.8050341846490525
1101	55	-1.1558130683317387
1101	56	-2.029205713982897
1101	57	-1.731095456014259
1101	58	-1.5877681276186557
1101	59	-1.1435382221351524
1101	60	-0.23144754605214146
1101	61	-0.030363643559862652
1102	1	0.1871012970573389
1102	2	0.10458164717717722
1102	3	-0.07720597540208729
1102	4	0.07370462849908677
1102	5	0.1974473553264886
1102	6	0.16524825020732692
1102	7	-0.021652290927285378
1102	8	0.15012883040563452
1102	9	-0.11149884494436435
1102	10	0.07779491038653674
1102	11	0.30921792095639233
1102	12	-0.11819180920628014
1102	13	-0.18241518451742422
1102	14	-0.20935730712945144
1102	15	-0.006180483737111331
1102	16	-0.05675309490239755
1102	17	-0.1707253942521021
1102	18	0.0031068736929640295
1102	19	-0.09437064373354076
1102	20	-1.6938373303111618
1102	21	0.09950405369720272
1102	22	-0.023638181236556477
1102	23	0.24763613314210176
1102	24	0.04341818187105417
1102	25	-0.26315005537998104
1102	26	-0.24454860152128077
1102	27	-0.0834839963370797
1102	28	0.0663884447320271
1102	29	0.0897675182689639
1102	30	0.09778863539628446
1102	31	0.09275831862068884
1102	32	-0.14818298896589255
1102	33	0.1347466054744615
1102	34	-0.04763417421577287
1102	35	-0.07197106368444395
1102	36	-0.07010540117319408
1102	37	-0.09275193768104373
1102	38	0.3827428578089922
1102	39	1.2524285123360173
1102	40	-0.12227889248501356
1102	41	-0.34176681543809906
1102	42	-1.2605249948964001
1102	43	-0.200988313453891
1102	44	-0.5566786941679354
1102	45	-1.186177529473369
1102	46	-0.8222479102042044
1102	47	-0.6704001609890113
1102	48	0.02223687020680387
1102	49	-0.2512527662001176
1102	50	0.07258254144180398
1102	51	0.34026265636762076
1102	52	0.43135305345310115
1102	53	-0.3301515033979783
1102	54	-0.6602626110449918
1102	55	-1.8056607840677756
1102	56	-0.09925647540421778
1102	57	-2.6011462174355806
1102	58	-1.9019813255881992
1102	59	-3.327903704368655
1102	60	-2.0733764800622936
1102	61	-1.766404252696919
1103	1	0.26666800339401675
1103	2	0.42581157941432224
1103	3	-0.01425209786098236
1103	4	-0.1674242666506487
1103	5	-0.3006460554038455
1103	6	0.11502115963826043
1103	7	-0.12463239925070013
1103	8	-0.687118880472859
1103	9	-0.2585855279647902
1103	10	0.037380075421879155
1103	11	-0.07870620407700102
1103	12	-0.44219054624268495
1103	13	-0.4142175792906997
1103	14	0.141163665352515
1103	15	-0.6811859484834244
1103	16	-0.36977376378735016
1103	17	-0.22046672959322677
1103	18	-0.2704704706832075
1103	19	-0.26244195260100156
1103	20	-1.2015729823161223
1103	21	-0.11617126750844164
1103	22	-0.20152174570841908
1103	23	-0.03011870619435797
1103	24	0.02612529575637268
1103	25	-0.030473301196408897
1103	26	-0.14117988901551826
1103	27	0.07147192137598779
1103	28	0.003823171373269929
1103	29	-0.2517184069733034
1103	30	-0.17063970356371527
1103	31	-0.10844149679233084
1103	32	-0.14983455674089186
1103	33	-0.13834498367376824
1103	34	-0.1867544714979701
1103	35	-0.05798131311975396
1103	36	0.05706531750166732
1103	37	-0.1546371537206781
1103	38	-0.05278527956258472
1103	39	1.4073844300490848
1103	40	-0.14821822165704646
1103	41	-0.12405132227510762
1103	42	5.927278830952787E-4
1103	43	-0.2285792104009161
1103	44	-0.2187873576124204
1103	45	-0.1420952425377564
1103	46	0.2551571853681338
1103	47	0.9025724181283721
1103	48	0.0945172481243759
1103	49	-0.36453088535587597
1103	50	0.25852964269748213
1103	51	-0.01249279016286664
1103	52	0.5559006938970867
1103	53	-0.23232040049073532
1103	54	0.010468207695438991
1103	55	1.29423477855822
1103	56	-0.3670047570450947
1103	57	0.595220333459423
1103	58	-0.6418351132645874
1103	59	1.3395239788217879
1103	60	1.2450117840913997
1103	61	0.22800956218176438
1104	1	-0.13998318804091525
1104	2	-0.6191455478773591
1104	3	0.20735953698671494
1104	4	-0.2711714834775947
1104	5	-0.4479765779297793
1104	6	-0.3696062504711719
1104	7	-0.2772687155139053
1104	8	-0.2075130251504831
1104	9	0.36304505614624816
1104	10	-0.3224664355450102
1104	11	0.15683313933102028
1104	12	0.176123916782565
1104	13	-0.08762702549847745
1104	14	-0.4278827129695273
1104	15	-0.1585652653624834
1104	16	-0.10258915058918205
1104	17	-0.020552001278289822
1104	18	0.03698132712815294
1104	19	0.2325128060327586
1104	20	-0.9321144575162705
1104	21	-0.11228666852223057
1104	22	-0.12837758918750097
1104	23	-0.04537286318900868
1104	24	-0.24456493116105804
1104	25	-0.11498701572575953
1104	26	0.06404063303669005
1104	27	-0.03483344600285676
1104	28	-0.30381811907156475
1104	29	0.2379305579231854
1104	30	-0.09218975330552581
1104	31	-0.2794585985025009
1104	32	-0.20005010461146355
1104	33	-0.2948658989231845
1104	34	-0.25942489643520616
1104	35	-0.1847518849137728
1104	36	-0.05653551626592446
1104	37	-0.01877093551991038
1104	38	0.008515587366211719
1104	39	0.8654354983138077
1104	40	-0.2275208887658593
1104	41	0.07387373338835346
1104	42	0.09998161414014106
1104	43	0.13695207957018596
1104	44	0.28535779374481507
1104	45	0.26228203729549193
1104	46	0.2725905076477346
1104	47	-0.09624325291241576
1104	48	-0.2436323563097531
1104	49	0.07945035487340846
1104	50	-0.5700268971670326
1104	51	-0.23590460502856558
1104	52	-0.2641286774611018
1104	53	-0.42493966241692505
1104	54	0.8500153203014378
1104	55	0.6198304960246972
1104	56	-0.0034631397254685226
1104	57	-0.08951129759842047
1104	58	-0.18875822662855768
1104	59	-0.9708649548084196
1104	60	-0.4530317044679819
1104	61	-0.980858693064814
1201	1	0.032888099087799105
1201	2	0.00600296697412972
1201	3	0.2192407251055286
1201	4	0.18070970464121672
1201	5	0.12232045177319151
1201	6	0.11712642279615437
1201	7	0.09144415577322462
1201	8	-0.09860213406137319
1201	9	0.12007475911654808
1201	10	-0.09276346524797674
1201	11	0.1528727432914181
1201	12	-0.0878364792570352
1201	13	0.1470264398480623
1201	14	-0.23778370306853702
1201	15	-0.0025256614020889856
1201	16	0.051470255351411254
1201	17	-0.023720318109972993
1201	18	0.11163479247468899
1201	19	0.07172072682483588
1201	20	0.27976673060253887
1201	21	0.18315887603223047
1201	22	0.3248897375451705
1201	23	0.25007268136545235
1201	24	-0.13109958462640492
1201	25	0.042636746650480006
1201	26	0.06245647462085202
1201	27	-0.2580017628345388
1201	28	0.15004326706705484
1201	29	0.09555432029942068
1201	30	-0.05733826815701093
1201	31	0.09519486684403944
1201	32	0.07638553895289135
1201	33	0.05840142780948554
1201	34	0.19602064811925146
1201	35	0.011683758650583798
1201	36	0.31811794908061586
1201	37	0.11608054962859882
1201	38	-0.0760388680793298
1201	39	1.4579107458385607
1201	40	0.18594445776879098
1201	41	-0.009888642849269047
1201	42	0.023942010179744955
1201	43	0.09180356471869544
1201	44	0.18159541750719654
1201	45	0.33158896798855864
1201	46	0.08922417101406666
1201	47	1.1455389253054094
1201	48	-0.03887988106222906
1201	49	0.40915332517043623
1201	50	-0.008838778355148236
1201	51	0.44310529596153003
1201	52	-0.08610887548091029
1201	53	0.5204068722365349
1201	54	0.43496581535094236
1201	55	0.264186931668263
1201	56	0.5107942860171022
1201	57	0.28890454398573695
1201	58	0.37223187238134514
1201	59	0.7964617778648488
1201	60	-2.1514475460521396
1201	61	-0.1903636435598628
1202	1	-0.3912144650147624
1202	2	0.024977325948491114
1202	3	-0.4371695313047326
1202	4	-0.3003159363844219
1202	5	-0.134089804637064
1202	6	-0.13005306438333264
1202	7	-0.0921455878165176
1202	8	-0.16526880072804317
1202	9	-0.26300216396037257
1202	10	-0.35943013191464956
1202	11	0.08107787149654655
1202	12	-0.3098877613083175
1202	13	-0.33246073963911726
1202	14	0.008370143085308257
1202	15	-0.008679507555932275
1202	16	0.08121384509500018
1202	17	-0.11397672836638151
1202	18	-0.5565703357304415
1202	19	-0.5293049142008002
1202	20	-0.3151050642692539
1202	21	-0.0465847137113613
1202	22	-0.3987000060445709
1202	23	-0.287363216070446
1202	24	-0.0013559948828145707
1202	25	-0.1850555610418283
1202	26	-0.2493383971740215
1202	27	0.03584439101161507
1202	28	-0.3873926303688435
1202	29	-0.01675337200826732
1202	30	0.053430962612218025
1202	31	-0.13454872289955233
1202	32	-0.007717025149673873
1202	33	-0.1959575465494865
1202	34	0.03191808401668794
1202	35	0.007581194548016867
1202	36	-0.34239487143220515
1202	37	0.12684978039783346
1202	38	-0.5411670732075393
1202	39	1.043551771479585
1202	40	0.015688047512384173
1202	41	-0.1652732582338814
1202	42	-1.2293913231535853
1202	43	-0.16870925579411988
1202	44	-0.4666097106979379
1202	45	-0.6376418012422107
1202	46	0.024608786398687243
1202	47	-1.4744610746945952
1202	48	-0.7086234708058186
1202	49	-0.08059026457315355
1202	50	-0.8016592911756604
1202	51	-0.14715111429487848
1202	52	-0.4866216959937262
1202	53	0.16861200044166935
1202	54	-0.4040085436234122
1202	55	-0.36965922217788716
1202	56	-0.14715443193161093
1202	57	-0.3310954560142605
1202	58	0.44043700058647417
1202	59	-1.0471279657248935
1202	60	1.460347325742731
1202	61	0.8004055872093687
1203	1	0.1489249654472431
1203	2	-0.18468827726550074
1203	3	0.11601491865391012
1203	4	-0.01228568706385147
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2403	28	-0.06554358739304433
2403	29	-0.2943987313437688
2403	30	-0.2909532916311903
2403	31	0.02984275416797999
2403	32	-0.1440839446151827
2403	33	-0.1661995111576502
2403	34	-0.08566949272582036
2403	35	-0.05366835402547565
2403	36	-0.028924304440515414
2403	37	-0.38654855835262225
2403	38	-0.2308745488305064
2403	39	1.0169717787024055
2403	40	-0.23133253753637462
2403	41	-0.5451938071685163
2403	42	0.1828152496163611
2403	43	-0.2308255432625259
2403	44	-0.5402825167651102
2403	45	-0.3022138489128494
2403	46	0.017674875239421795
2403	47	0.523660991033104
2403	48	-0.5216967824706771
2403	49	-0.31807672177792057
2403	50	0.16562131554156423
2403	51	-0.4467538589680444
2403	52	-0.33840934496447517
2403	53	-0.1333020479512541
2403	54	-0.7530154053063356
2403	55	-0.1538412373458229
2403	56	0.7022496850781366
2403	57	0.9045852951594497
2403	58	-0.015467658135086992
2403	59	2.014771637019777
2403	60	2.2242332051215676
2403	61	0.22315748320070128
2404	1	-0.0011859749862779267
2404	2	-0.07844147747031371
2404	3	-0.5733518674870695
2404	4	0.04070970464121615
2404	5	0.09639452584726627
2404	6	-0.6169476512779184
2404	7	0.11070341503248216
2404	8	-0.04304657850581606
2404	9	-0.3399252408834528
2404	10	-0.3112819837664986
2404	11	0.05287274329141667
2404	12	0.0721635207429614
2404	13	0.002581995403616588
2404	14	0.13629037100553631
2404	15	0.015252116375691571
2404	16	-0.43445567057451484
2404	17	-0.055572169961827456
2404	18	-0.0024392815993863337
2404	19	0.16357257867669261
2404	20	0.32421117504698316
2404	21	-0.3049892721159182
2404	22	0.026371219026650294
2404	23	-0.33066805937529153
2404	24	0.05704856352173948
2404	25	0.04856267257640212
2404	26	-0.14791389574952518
2404	27	-0.01429805913083726
2404	28	-0.06032710330332236
2404	29	0.17925802400312563
2404	30	-0.003264194082937877
2404	31	-0.11517550352633776
2404	32	0.008237390804744393
2404	33	-0.547524498116438
2404	34	-0.02620157410296997
2404	35	-0.2727606857938625
2404	36	-0.1700301990675328
2404	37	-0.05947500592695576
2404	38	-0.11011294215340683
2404	39	1.3216144495422597
2404	40	1.853184286915166E-5
2404	41	0.1167780238173961
2404	42	0.03727534351308037
2404	43	-0.4037519908368594
2404	44	0.18011393602571246
2404	45	0.46121859761818484
2404	46	0.3314463932362912
2404	47	0.5959092956757814
2404	48	0.09593493375258788
2404	49	-0.1315874155703014
2404	50	0.051901962385592526
2404	51	0.36310529596153174
2404	52	0.13759482822279523
2404	53	0.04040687223653805
2404	54	0.05866951905464646
2404	55	-0.5565538090724758
2404	56	0.5893128045356235
2404	57	1.3281638032449976
2404	58	1.193713353862826
2404	59	2.77794325934633
2404	60	0.6292931946886
2404	61	1.265932652736435
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	19.0
19	15.0
20	11.0
21	16.0
22	27.0
23	18.0
24	61.0
25	74.0
26	115.0
27	152.0
28	254.0
29	289.0
30	383.0
31	491.0
32	722.0
33	865.0
34	1165.0
35	1665.0
36	3001.0
37	8950.0
38	31102.0
39	15371.0
40	546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.778988946385376	94.46400685875257	1.8631923818855447	1.8938118129765151
2	94.60568837971543	1.8838737345116479	2.0018072935014017	1.5086305922715229
3	2.32864338533613	94.27407872376257	1.462100218932284	1.9351776719690128
4	1.7606172877307937	2.140298233258826	94.41654674056156	1.6825377384488198
5	1.6702899660124317	1.7866438041581187	1.9872010778039744	94.55586515202548
6	94.55892709513456	1.7912367188217642	1.5968033313941026	2.0530328546495604
7	95.21265194892679	1.8846259836492236	1.4391132612756055	1.4636088061483818
8	2.431182828623044	95.5004746011819	0.8037600661379712	1.2645825040570746
9	2.9853945313696073	95.05036896414465	1.0043173397838268	0.9599191647019198
10	93.98328179062433	4.208640803453871	0.9384855629382407	0.8695918429835574
11	2.330138706022842	95.05036896414465	1.938209988058422	0.6812823417740899
12	94.23742306867938	2.5766251263051534	0.9492023638200802	2.2367494411953825
13	3.3206773018157323	2.536819865886892	0.7700786919379038	93.37242414035947
14	2.8965981812057935	94.58801555467099	0.7348663461832879	1.7805199179399247
15	1.899935699194709	3.2043234636700455	1.339600110229952	93.55614072690528
16	2.0438470253222696	95.11620074099024	0.5848311338375333	2.2551210998499647
17	2.3913775682047826	3.0129520193514803	1.0242199699929575	93.57145044245078
18	94.03992773814262	3.026730763342417	0.8313175541198444	2.102023944395113
19	3.078783796197067	2.7557487981873297	92.71410637190361	1.4513610337119935
20	2.39443951131388	2.039254110658624	1.906059585412903	93.6602467926146
21	2.31176704736826	2.85832389234208	92.91007073088582	1.9198383294038397
22	94.33081233350684	2.5215101503414066	1.1987507272114883	1.9489267889402615
23	2.1923512661134756	95.47904099941823	0.6154505649285037	1.71315716953979
24	2.0499709115404636	94.65078538840748	1.108423405493126	2.190820294558927
25	1.541688355430356	3.1262439143880707	0.8052910376925198	94.52677669248905
26	2.18775835144983	2.0515018830950122	92.94375210508589	2.8169876603692705
27	2.14642211947702	1.5080069812302888	1.6243608193759762	94.72121007991672
28	1.2263082151933618	2.7281913102054562	1.6121130469395877	94.4333874276616
29	1.9519887320493585	93.69392816681466	1.278361248048011	3.0757218530879697
30	93.62962736152363	2.715943537769068	1.6794757953397226	1.9749533053675863
31	2.060687712422303	2.8445451483511435	92.9973361094951	2.0974310297314678
32	2.219908754095349	2.2551210998499647	1.9810771915857803	93.54389295446892
33	1.0563703726384763	93.40304357145044	1.7192810557579838	3.821305000153097
34	0.9706359655837594	3.0068281331332862	1.333476224011758	94.68905967727119
35	1.4069628586300866	1.6641660797942375	1.431458403502863	95.49741265807282
36	92.5686640742215	3.008359104687835	1.1283260357022566	3.2946507853884075
37	1.8662543249946415	93.84396337916041	0.9813527664655991	3.308429529379344
38	2.0974310297314678	94.19914877981567	1.1834410116660032	2.5199791787868584
39	1.5544532558213011	3.2431834697165964	1.7613977441471043	93.440965530315
40	0.9492023638200802	94.21292752380661	1.1466976943568388	3.691172418016473
41	0.8435653265562325	94.1685293487247	1.6626351082396889	3.3252702164793777
42	1.5753697296304234	3.3390489604703144	0.8680608714290088	94.21752043847025
43	0.9966624820110842	94.45788297253436	1.2370250160752014	3.308429529379344
44	1.064025230411219	3.4079426804249975	92.35892097124835	3.169111117915429
45	1.1222021494840626	2.523041121895955	2.0499709115404636	94.30478581707952
46	1.5860865305122631	2.2903334456045807	93.06316788634066	3.0604121375424844
47	92.32370862549374	3.169111117915429	1.405431887075538	3.1017483695152945
48	93.62197250375088	2.6439878747052883	0.8190697816834563	2.914969839860375
49	94.03839676658808	3.259438439633792	0.4837870112373312	2.2183777825408004
50	94.96463455708994	2.679200220459904	0.4746011819100401	1.8815640405401266
51	94.04452065280627	2.5781560978597016	1.615174990048685	1.7621482592853424
52	94.13178603141553	2.5383508374414405	0.8481582412198782	2.481704889923145
53	94.05829939679721	2.9119078967512784	1.5156618390030314	1.5141308674484828
54	94.26344958510671	2.163262806577054	1.6228298478214276	1.9504577604948101
55	94.64772344529838	2.0469089684313664	1.4161486879573777	1.8892188983128695
56	95.84035028629168	1.6243608193759762	1.3640956551027281	1.1711932392296152
57	95.26164303867233	2.27043081539545	1.4957592087939005	0.9721669371383079
58	95.35656327505436	1.8922808414219665	1.855537524112802	0.8956183594108821
59	95.21112097737225	0.8971493309654306	2.2535901282954165	1.6381395633669127
60	95.21724486359044	1.6136440184941363	2.5628463823142167	0.6062647356012125
61	94.90492666646254	1.2814231911571083	3.091031568633455	0.7226185737468998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	0.5
28	0.0
29	1.0
30	1.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	4.5
43	9.5
44	10.0
45	32.5
46	55.0
47	139.0
48	303.5
49	384.0
50	513.5
51	643.0
52	759.0
53	877.5
54	880.0
55	849.0
56	818.0
57	830.0
58	1134.5
59	1427.0
60	3967.5
61	29245.5
62	51983.0
63	26277.5
64	572.0
65	298.0
66	17.0
67	10.0
68	10.0
69	10.0
70	12.5
71	13.0
72	11.0
73	8.0
74	5.0
75	5.0
76	3.0
77	1.0
78	3.0
79	5.0
80	2.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.041336231972809945
3	0.0015309715545485164
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.086438653969809
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	65318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.028965981812058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.93397663788726	4.2162956612266145
2	14.169629253428138	1.7085642548761444
3	6.2722194007110215	1.1344499219204507
4	2.5393600812595225	0.6123886218194067
5	1.4220416455053326	0.42867203527358466
6	0.8633824276282377	0.3123181971278974
7	0.5332656170644997	0.22505281851863193
8	0.5840528186896902	0.28169876603692706
9	0.25393600812595224	0.13778743990936648
>10	2.717115286947689	3.1415536299335556
>50	0.35551041137633316	1.6136440184941363
>100	0.25393600812595224	2.8889433234330504
>500	0.025393600812595223	0.9492023638200802
>1k	0.050787201625190445	4.129030282617349
>5k	0.0	0.0
>10k+	0.025393600812595223	78.22039866499281
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG	51092	78.22039866499281	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAANAACATCTGGGGGGGGGGGGGGG	1672	2.5597844392051194	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGT	1025	1.5692458434122294	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGTG	620	0.9492023638200802	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGTGG	472	0.7226185737468998	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGAACATCTGGGGGGGGGGGGGGG	246	0.37661900241893503	TruSeq Adapter, Index 2 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGTGGG	238	0.3643712299825469	TruSeq Adapter, Index 22 (97% over 38bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGG	205	0.31384916868244583	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG	160	0.24495544872776262	TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGTGGGG	130	0.19902630209130714	TruSeq Adapter, Index 22 (97% over 38bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGG	114	0.1745307572185309	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGAAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG	113	0.17299978566398236	TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAACACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG	105	0.16075201322759422	TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTAGGGGGGGGGGGGGG	104	0.1592210416730457	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAAAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG	100	0.15309715545485167	TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAGCTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG	100	0.15309715545485167	TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAATCACCGAACAACATCTGGGGGGGGGGGGGGG	97	0.14850424079120608	TruSeq Adapter, Index 2 (97% over 36bp)
TTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATGTGGGGGG	94	0.14391132612756055	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG	86	0.1316635536911724	TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACATCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG	81	0.12400869591842985	TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGTGT	80	0.12247772436388131	TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACCGAACAACATCTGGGGGGGGGGGGGGGG	77	0.11788480970023577	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	90.9152147953091	0.0	0.0	0.0	0.0
2	91.63324045439236	0.0	0.0	0.0	0.0
3	92.01751431458403	0.0	0.0	0.0	0.0
4	92.13386815272972	0.0	0.0	0.0	0.0
5	92.22266450289354	0.0	0.0	0.0	0.0
6	92.38035457301203	0.0	0.0	0.0	0.0
7	92.5778499035488	0.0	0.0	0.0	0.0
8	93.36476928258674	0.0	0.0	0.0	0.0
9	93.83783949294222	0.0	0.0	0.0	0.0
10	94.41807771211612	0.0	0.0	0.0	0.0
11	94.83297100339875	0.0	0.0	0.0	0.0
12	95.24633332312686	0.0	0.0	0.0	0.0
13	95.60458066689121	0.0	0.0	0.0	0.0
14	95.94292538044643	0.0	0.0	0.0	0.0
15	96.31801341131082	0.0	0.0	0.0	0.0
16	96.55225205915674	0.0	0.0	0.0	0.0
17	96.75587127591169	0.0	0.0	0.0	0.0
18	96.81711013809364	0.0	0.0	0.0	0.0
19	96.89672065893016	0.0	0.0	0.0	0.0
20	96.96255243577575	0.0	0.0	0.0	0.0
21	97.72191432683181	0.0	0.0	0.0	0.0
22	97.74640987170459	0.0	0.0	0.0	0.0
23	97.78315318901375	0.0	0.0	0.0	0.0
24	97.81377262010471	0.0	0.0	0.0	0.0
25	97.82448942098655	0.0	0.0	0.0	0.0
26	97.83826816497749	0.0	0.0	0.0	0.0
27	97.83979913653204	0.0	0.0	0.0	0.0
28	97.85051593741389	0.0	0.0	0.0	0.0
29	97.86888759606846	0.0	0.0	0.0	0.0
30	97.88113536850486	0.0	0.0	0.0	0.0
31	97.88419731161395	0.0	0.0	0.0	0.0
32	97.88725925472305	0.0	0.0	0.0	0.0
33	97.89491411249578	0.0	0.0	0.0	0.0
34	97.89491411249578	0.0	0.0	0.0	0.0
35	97.89491411249578	0.0	0.0	0.0	0.0
36	97.89491411249578	0.0	0.0	0.0	0.0
37	97.89491411249578	0.0	0.0	0.0	0.0
38	97.89491411249578	0.0	0.0	0.0	0.0
39	97.89491411249578	0.0	0.0	0.0	0.0
40	97.89491411249578	0.0	0.0	0.0	0.0
41	97.89491411249578	0.0	0.0	0.0	0.0
42	97.89644508405034	0.0	0.0	0.0	0.0
43	97.90103799871399	0.0	0.0	0.0	0.0
44	97.92247160047766	0.0	0.0	0.0	0.0
45	97.99902017820509	0.0	0.0	0.0	0.0
46	98.00208212131419	0.0	0.0	0.0	0.0
47	98.00361309286873	0.0	0.0	0.0	0.0
48	98.00361309286873	0.0	0.0	0.0	0.0
49	98.00361309286873	0.0	0.0	0.0	0.0
50	98.00361309286873	0.0	0.0	0.0	0.0
>>END_MODULE