FastQCFastQC Report
Sat 10 Aug 2024
AM-AL-EC3_S25_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-EC3_S25_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65318
Sequences flagged as poor quality0
Sequence length61
%GC61

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG5109278.22039866499281TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAANAACATCTGGGGGGGGGGGGGGG16722.5597844392051194TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGT10251.5692458434122294TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGTG6200.9492023638200802TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGTGG4720.7226185737468998TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAGAACATCTGGGGGGGGGGGGGGG2460.37661900241893503TruSeq Adapter, Index 2 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGTGGG2380.3643712299825469TruSeq Adapter, Index 22 (97% over 38bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGG2050.31384916868244583TruSeq Adapter, Index 22 (97% over 38bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG1600.24495544872776262TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGTGGGG1300.19902630209130714TruSeq Adapter, Index 22 (97% over 38bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGG1140.1745307572185309TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGAAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG1130.17299978566398236TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAACACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG1050.16075201322759422TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTAGGGGGGGGGGGGGG1040.1592210416730457TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAAAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG1000.15309715545485167TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAGCTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG1000.15309715545485167TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAATCACCGAACAACATCTGGGGGGGGGGGGGGG970.14850424079120608TruSeq Adapter, Index 2 (97% over 36bp)
TTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATGTGGGGGG940.14391132612756055TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG860.1316635536911724TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACATCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGGGG810.12400869591842985TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACAACATCTGGGGGGGGGGGGTGT800.12247772436388131TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACCGAACAACATCTGGGGGGGGGGGGGGGG770.11788480970023577Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)