##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-EC2_S86_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	59900
Sequences flagged as poor quality	0
Sequence length	61
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.518864774624376	40.0	40.0	40.0	40.0	40.0
2	39.441402337228716	40.0	40.0	40.0	40.0	40.0
3	39.17348914858097	40.0	40.0	40.0	40.0	40.0
4	39.49776293823039	40.0	40.0	40.0	40.0	40.0
5	39.24467445742905	40.0	40.0	40.0	40.0	40.0
6	39.32707846410685	40.0	40.0	40.0	40.0	40.0
7	39.232587646076794	40.0	40.0	40.0	40.0	40.0
8	38.95732888146912	40.0	40.0	40.0	40.0	40.0
9	39.24494156928214	40.0	40.0	40.0	40.0	40.0
10	39.36006677796327	40.0	40.0	40.0	40.0	40.0
11	39.36387312186978	40.0	40.0	40.0	40.0	40.0
12	39.385108514190314	40.0	40.0	40.0	40.0	40.0
13	38.44647746243739	40.0	40.0	40.0	40.0	40.0
14	39.34938230383973	40.0	40.0	40.0	40.0	40.0
15	39.43312186978297	40.0	40.0	40.0	40.0	40.0
16	39.15465776293823	40.0	40.0	40.0	40.0	40.0
17	39.31278797996661	40.0	40.0	40.0	40.0	40.0
18	39.291151919866444	40.0	40.0	40.0	40.0	40.0
19	39.28100166944908	40.0	40.0	40.0	40.0	40.0
20	25.285275459098496	24.0	12.0	40.0	12.0	40.0
21	26.545041736227045	24.0	12.0	40.0	12.0	40.0
22	27.132220367278798	24.0	12.0	40.0	12.0	40.0
23	36.446010016694494	40.0	40.0	40.0	24.0	40.0
24	38.385308848080136	40.0	40.0	40.0	40.0	40.0
25	26.210016694490818	24.0	12.0	40.0	12.0	40.0
26	37.75926544240401	40.0	40.0	40.0	24.0	40.0
27	38.1367612687813	40.0	40.0	40.0	40.0	40.0
28	22.881803005008347	12.0	12.0	40.0	12.0	40.0
29	33.98063439065108	40.0	24.0	40.0	24.0	40.0
30	21.9779632721202	12.0	12.0	40.0	12.0	40.0
31	23.36247078464107	24.0	12.0	40.0	12.0	40.0
32	26.582170283806345	24.0	12.0	40.0	12.0	40.0
33	31.883739565943237	40.0	24.0	40.0	24.0	40.0
34	36.63405676126878	40.0	40.0	40.0	24.0	40.0
35	21.71806343906511	12.0	12.0	40.0	12.0	40.0
36	25.52874791318865	24.0	12.0	40.0	12.0	40.0
37	21.070116861435725	12.0	12.0	40.0	12.0	40.0
38	32.02597662771286	40.0	24.0	40.0	12.0	40.0
39	24.763606010016694	24.0	12.0	40.0	12.0	40.0
40	19.417228714524207	12.0	12.0	24.0	12.0	40.0
41	26.31846410684474	24.0	24.0	40.0	12.0	40.0
42	19.53662771285476	12.0	12.0	24.0	12.0	40.0
43	26.897495826377295	24.0	24.0	40.0	12.0	40.0
44	19.110183639398997	12.0	12.0	24.0	12.0	40.0
45	26.394858096828045	24.0	24.0	40.0	12.0	40.0
46	21.11585976627713	12.0	12.0	24.0	12.0	40.0
47	18.33756260434057	12.0	12.0	24.0	12.0	40.0
48	24.306777963272122	24.0	24.0	24.0	12.0	40.0
49	18.62524207011686	12.0	12.0	24.0	12.0	40.0
50	24.617896494156927	24.0	24.0	24.0	12.0	40.0
51	17.887078464106846	12.0	12.0	24.0	12.0	40.0
52	24.766677796327212	24.0	24.0	24.0	12.0	40.0
53	18.197929883138563	12.0	12.0	24.0	12.0	40.0
54	17.724540901502504	12.0	12.0	24.0	12.0	40.0
55	23.015959933222035	24.0	12.0	24.0	12.0	40.0
56	17.718764607679464	12.0	12.0	24.0	12.0	40.0
57	20.882070116861435	24.0	12.0	24.0	12.0	40.0
58	22.749515859766277	24.0	12.0	24.0	12.0	40.0
59	19.259632721202003	12.0	12.0	24.0	12.0	40.0
60	17.14951585976628	12.0	12.0	24.0	12.0	40.0
61	21.340801335559267	24.0	12.0	24.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.30430906612122044
1101	2	0.32022765677820786
1101	3	0.5622741585417614
1101	4	-0.04138718509496897
1101	5	0.3830040019445988
1101	6	0.14893042532902
1101	7	0.3435530626334895
1101	8	0.65006475322771
1101	9	-0.27423085817851955
1101	10	0.2857362865076851
1101	11	0.23544617775892362
1101	12	0.05987233436920292
1101	13	0.6284175753346872
1101	14	0.012103023060880957
1101	15	-0.11773386725334944
1101	16	0.6466094959896935
1101	17	0.24225165521708192
1101	18	0.1961440789031741
1101	19	0.36656711658226726
1101	20	-0.7562285078086255
1101	21	-1.0695375615130196
1101	22	-0.014144220682556607
1101	23	0.8164414996981719
1101	24	-0.057430530195546226
1101	25	-0.4498681593595677
1101	26	0.7080025195301829
1101	27	0.26868469871323697
1101	28	0.20787346915068028
1101	29	0.7748667390533086
1101	30	-0.8117030618303289
1101	31	-0.21720596627719857
1101	32	0.683868696128652
1101	33	1.152004783758123
1101	34	0.6646247526830038
1101	35	-0.3094550757781782
1101	36	1.230024929855908
1101	37	-0.6449062363505931
1101	38	1.1703989495437384
1101	39	-0.056713981969700455
1101	40	-0.018180714089957917
1101	41	1.0305400718480549
1101	42	-0.570133371436043
1101	43	0.7577828073890132
1101	44	-0.5703701484048835
1101	45	0.47771946237343244
1101	46	-0.21403483573483584
1101	47	-0.39992755976369665
1101	48	0.7379953477262475
1101	49	-0.4397903170204316
1101	50	0.14727380810617063
1101	51	0.09737028108167678
1101	52	0.3226074550894431
1101	53	0.1336492613013469
1101	54	-0.021578547637282952
1101	55	0.8005506812024024
1101	56	-0.11959920128926882
1101	57	0.02079450810220962
1101	58	-0.1606374491134659
1101	59	-0.4654647846884359
1101	60	0.23351797839590205
1101	61	-0.9874272002828981
1102	1	0.07228719520715998
1102	2	0.0658594171490634
1102	3	-0.14650986618188
1102	4	-0.13410084252988952
1102	5	-0.14807884602877408
1102	6	0.10304905126507435
1102	7	-0.056660891960589765
1102	8	0.5371442730185123
1102	9	0.12348695948462307
1102	10	4.6349440023618627E-4
1102	11	0.3715450723268532
1102	12	0.12037393902866
1102	13	0.24615203391070395
1102	14	-0.2668699948875357
1102	15	0.1842987013904107
1102	16	-0.12172522408638287
1102	17	0.043155019058758626
1102	18	0.03639131532300155
1102	19	0.060017728730137776
1102	20	-2.1894865608218197
1102	21	-2.7898513615843363
1102	22	-1.541423431427834
1102	23	-1.0050252779079116
1102	24	-0.3164344526964413
1102	25	-1.1783835522039752
1102	26	-0.5129650306897133
1102	27	0.1671751301883333
1102	28	-1.0555284088951993
1102	29	-0.24914372094906412
1102	30	-0.8136048804443767
1102	31	-1.1879655050861864
1102	32	-0.3038883465434026
1102	33	-0.722712915041356
1102	34	0.3117185361134105
1102	35	-1.1423327650685628
1102	36	-0.6527754980532805
1102	37	-0.8619512856790159
1102	38	-0.6319307782584715
1102	39	-0.5428663651861498
1102	40	-0.4036555744251551
1102	41	1.0856928116554982
1102	42	-0.7821861469025855
1102	43	0.28197156288645786
1102	44	-0.8070277021906911
1102	45	-1.1709195486808888
1102	46	-1.6493135710254556
1102	47	-0.9176976774387242
1102	48	0.0891186093451708
1102	49	-0.8542679112198854
1102	50	0.4698697905143483
1102	51	-0.260765936676556
1102	52	0.26008516815256044
1102	53	-0.36058585102481544
1102	54	0.12355398534738526
1102	55	-0.3306748031670246
1102	56	-0.7406618424398701
1102	57	0.01675314354735491
1102	58	0.19333854042653442
1102	59	-0.22963927654627625
1102	60	-0.5993597108944826
1102	61	-0.836232620465946
1103	1	0.07661607291635875
1103	2	0.15536188870424184
1103	3	0.21126602664914884
1103	4	0.1341725787459609
1103	5	0.07421487417363437
1103	6	0.0515705055339879
1103	7	0.2870753974892324
1103	8	0.720244100448383
1103	9	0.39035642042234997
1103	10	-0.14870729152506357
1103	11	-0.21893724654763957
1103	12	0.06889539329757355
1103	13	-0.2992865574490935
1103	14	0.16905969009886235
1103	15	-0.19114369236196183
1103	16	0.04195315199949334
1103	17	-0.0027076853198408912
1103	18	0.07851012546661451
1103	19	0.22498306845928795
1103	20	-1.0554375977050299
1103	21	-0.29477984735461504
1103	22	0.10694300222041875
1103	23	0.12890668851403575
1103	24	-0.07425129437066147
1103	25	-1.8348520069701273
1103	26	-0.29277725099449015
1103	27	-0.10449119523796924
1103	28	-0.9681318778472026
1103	29	-2.158295787403187
1103	30	-0.4899920225076144
1103	31	-1.2891676461257013
1103	32	-0.6829576378359228
1103	33	-0.4543224970706632
1103	34	-0.11169250252475393
1103	35	-0.8101791484082348
1103	36	-1.3231665595057933
1103	37	-1.4008380621275798
1103	38	-0.18629036760007267
1103	39	-2.0130468325879463
1103	40	-1.1333639601632548
1103	41	0.4129618163061153
1103	42	-0.5527556283147348
1103	43	-1.5787438719025104
1103	44	-1.15263593875801
1103	45	-0.31965159700052226
1103	46	-0.33111181022519887
1103	47	0.01312802673513147
1103	48	-1.3280623552925768
1103	49	-1.1829116270349118
1103	50	-0.2815999804646232
1103	51	-0.7052363803865198
1103	52	0.28629242779747344
1103	53	-1.0941551276927513
1103	54	-0.3182255343811846
1103	55	-0.3469373437502554
1103	56	-0.43518347220382836
1103	57	-0.3479255542794313
1103	58	0.008015775794632418
1103	59	-0.4832881109667042
1103	60	-0.18343646072629838
1103	61	-0.280732313349624
1104	1	0.005134076976624158
1104	2	6.445043683029894E-4
1104	3	0.1263210538956514
1104	4	0.08149601073655788
1104	5	-0.15412148654866797
1104	6	0.004770633752812614
1104	7	-0.1909671284216543
1104	8	0.3018233289984451
1104	9	-0.09142582127013554
1104	10	0.3812638592389064
1104	11	0.10908925200990183
1104	12	0.06291184804701544
1104	13	-0.2925550690422085
1104	14	-0.0017918966091201582
1104	15	0.3785752947093073
1104	16	0.20457736398795134
1104	17	0.1567110559986702
1104	18	0.2169864584081651
1104	19	0.03265482826268595
1104	20	-0.5677786597843095
1104	21	-1.1038406444483186
1104	22	-1.134856335315643
1104	23	-0.4532067851476782
1104	24	-0.17597156363019906
1104	25	-0.042139110293131665
1104	26	0.22298297979939719
1104	27	-0.1568472161803811
1104	28	-1.7869159359730702
1104	29	-1.1738957446635823
1104	30	-0.8056240344747039
1104	31	0.4597371513954016
1104	32	0.9891227126288697
1104	33	-1.3760021637017132
1104	34	-0.06702961119020756
1104	35	-0.4609965434290686
1104	36	-1.3231665595057933
1104	37	-0.8541984638799036
1104	38	-1.790521588884399
1104	39	-0.7731707774538492
1104	40	0.1859009185631031
1104	41	-0.20697621126093324
1104	42	0.2913516481036851
1104	43	-0.8106062503617473
1104	44	0.06619085897679255
1104	45	-1.3494624742309966
1104	46	-0.5726974497165997
1104	47	-0.24491236219530776
1104	48	-0.9098552818872996
1104	49	0.09169036035690326
1104	50	-0.6951056968873175
1104	51	0.04569854855888167
1104	52	-0.901013908349551
1104	53	0.10629363820104132
1104	54	-0.5951782002642894
1104	55	-0.555506635341235
1104	56	-0.01451887128492757
1104	57	-0.5020018444813772
1104	58	-0.5318923340461623
1104	59	-0.709060095153049
1104	60	-0.22414593823458162
1104	61	0.019086043312412926
1201	1	-0.44167928523977196
1201	2	-0.7609562253090374
1201	3	-0.43701265947796486
1201	4	-0.3352181609656455
1201	5	-1.1536654382075469
1201	6	-0.09806826717068162
1201	7	-0.23543184168126174
1201	8	-0.06620768228875562
1201	9	-0.7703677891187297
1201	10	-0.24534656146568778
1201	11	-0.29884598912565963
1201	12	-0.7064417034294408
1201	13	-0.401892658826732
1201	14	-0.6914510004741246
1201	15	-0.3743605164866821
1201	16	-0.054686117941130874
1201	17	-0.5378505675334253
1201	18	-0.4775751651239304
1201	19	-0.2751778019961222
1201	20	-0.7816653317715421
1201	21	-0.37644354035528593
1201	22	-0.6252223141093971
1201	23	-0.04413142315930685
1201	24	-0.003585290685265363
1201	25	-1.3571545019246471
1201	26	-0.40123318711430045
1201	27	-0.23505950668317155
1201	28	-0.8991038278896113
1201	29	-2.550819698602698
1201	30	0.39309903017014847
1201	31	0.6891294669808516
1201	32	-0.549698402409323
1201	33	-1.4601430826391208
1201	34	0.5351822232642434
1201	35	-0.8518299717724744
1201	36	0.7326993622819167
1201	37	-1.2641859225505243
1201	38	-0.31908161624729914
1201	39	-0.9897937394878262
1201	40	-0.7388511051514115
1201	41	-0.6573239481210393
1201	42	0.6926741882929477
1201	43	-1.8632798337615881
1201	44	-0.18132937816834627
1201	45	-0.23237206264736798
1201	46	-1.6493135710254556
1201	47	-0.6495412528577198
1201	48	-0.17903781523583362
1201	49	-0.541418749208713
1201	50	-1.943538030714702
1201	51	0.2755469124854564
1201	52	-0.5220377368753724
1201	53	-0.4946640633153194
1201	54	0.7045595719395621
1201	55	-0.5094457528876966
1201	56	-0.4948517865739497
1201	57	-0.2290569123185655
1201	58	-1.0357117389030748
1201	59	-1.5257286620211374
1201	60	-0.37589602374364617
1201	61	-1.1714281511922024
1202	1	-0.15174079418395792
1202	2	0.16720809950332693
1202	3	0.2753624886513677
1202	4	0.08541272893899077
1202	5	-0.024045213615252692
1202	6	0.15236906706553555
1202	7	0.3437679777420257
1202	8	0.7949175363783638
1202	9	0.3812602228444746
1202	10	0.020101212361971932
1202	11	0.2558631130694806
1202	12	0.10908789422305887
1202	13	0.5948908184036767
1202	14	0.34844516791365976
1202	15	-0.08442202543086808
1202	16	-0.1730767279518517
1202	17	0.48489081274986034
1202	18	0.04011571010885007
1202	19	0.22106958810601895
1202	20	0.2749341829285328
1202	21	-0.5513772348420538
1202	22	0.6647726071704412
1202	23	0.2959171068333575
1202	24	0.5080111203524851
1202	25	-0.4453549420803924
1202	26	-0.6042691331670014
1202	27	0.10577904644588187
1202	28	0.38603809412381906
1202	29	-0.9193090615054658
1202	30	0.6200613912107222
1202	31	-7.864519853448826E-4
1202	32	0.10703654482841429
1202	33	0.6804810356386284
1202	34	0.6532342519307974
1202	35	0.7000011889972342
1202	36	0.8788536425144073
1202	37	1.1668281285752578
1202	38	-0.17518454497592728
1202	39	1.0870642256382936
1202	40	0.6787889828797375
1202	41	1.0851285094152168
1202	42	0.49584556688331816
1202	43	0.1807357409802428
1202	44	0.883960391032037
1202	45	0.25721656101946877
1202	46	-0.4962183732375216
1202	47	0.645419528136582
1202	48	0.6939377504771613
1202	49	1.137041834279806
1202	50	0.2564506832404909
1202	51	1.058685561545893
1202	52	0.4347931417432136
1202	53	-0.03656350465610103
1202	54	0.485836023607078
1202	55	0.9559343046711319
1202	56	0.8185052474534764
1202	57	0.5860212435417118
1202	58	0.37493100134860313
1202	59	0.2583693008477752
1202	60	0.45509545529252904
1202	61	-0.13593354027860016
1203	1	-0.10301827487320736
1203	2	-0.1726611815424235
1203	3	0.0044177471183957095
1203	4	-0.08689861984294822
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2403	32	0.9412806249741337
2403	33	1.7896050627954452
2403	34	1.2500217995380183
2403	35	-0.9539150222960266
2403	36	-0.42699169611781684
2403	37	0.23289867005563636
2403	38	1.483037014884978
2403	39	0.35184316254838066
2403	40	-0.0017872714670055245
2403	41	1.011937583765274
2403	42	-0.09763017413340691
2403	43	0.6546552640800947
2403	44	0.06362589856639644
2403	45	0.3784286822315899
2403	46	2.168130028815799
2403	47	-0.178557951578604
2403	48	1.2427041025222039
2403	49	0.20443709839637947
2403	50	0.3395770286619175
2403	51	-0.07295875042628808
2403	52	-3.7824424784460575E-4
2403	53	0.49895562102695834
2403	54	-1.0501798730645575
2403	55	0.48698249145702377
2403	56	0.6843770805384253
2403	57	0.6549068206240101
2403	58	0.5223063946256339
2403	59	1.1757397264149247
2403	60	-0.06121519216566185
2403	61	0.6175024590590397
2404	1	-0.23976388220705047
2404	2	0.021052667633611577
2404	3	0.08550472736504133
2404	4	0.14272050053247654
2404	5	0.39689892161459994
2404	6	0.004770633752812614
2404	7	0.2171961368844677
2404	8	0.5059049616515026
2404	9	-0.4179564335150374
2404	10	0.013916920463394433
2404	11	-0.15621687043907428
2404	12	0.10372817457762551
2404	13	0.1156081962639135
2404	14	-0.12424087620095037
2404	15	0.2153099885868599
2404	16	-0.6117491666242927
2404	17	0.013853913141531393
2404	18	-0.17076864363264832
2404	19	0.05306299152799454
2404	20	-0.5065541699883944
2404	21	0.569628743306783
2404	22	-1.1960808251115616
2404	23	-0.9021863769844174
2404	24	-0.033114420773053155
2404	25	-0.42989421233394864
2404	26	0.018901347146332625
2404	27	0.006418089942066274
2404	28	-0.46038532372817187
2404	29	1.0710022145200924
2404	30	-0.47909342222980555
2404	31	-0.31577305268623235
2404	32	-0.8272038179833743
2404	33	-0.45763481676293694
2404	34	-0.8425398152718415
2404	35	-0.420180216898455
2404	36	-0.9150032941996713
2404	37	-0.20113723939010697
2404	38	-0.586439956231338
2404	39	1.4717271817298219
2404	40	0.08386010223657436
2404	41	-1.3498333541180756
2404	42	-0.361709576386108
2404	43	-0.5453001279127676
2404	44	-0.7297275083701464
2404	45	0.4668640563812474
2404	46	-0.36861581706353874
2404	47	0.20406722964142787
2404	48	0.04932839158209035
2404	49	-0.3572892314798324
2404	50	0.4273432827045198
2404	51	0.1477393648854104
2404	52	0.42551670389534735
2404	53	-0.3835022801663044
2404	54	-0.3298720778153097
2404	55	0.4240852013934564
2404	56	0.3732362307558894
2404	57	0.4979981555186228
2404	58	1.161985216974248
2404	59	1.250123578316341
2404	60	-0.36700308109172397
2404	61	0.9170452269858842
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
12	1.0
13	0.0
14	2.0
15	20.0
16	54.0
17	109.0
18	122.0
19	187.0
20	251.0
21	361.0
22	607.0
23	913.0
24	1845.0
25	5384.0
26	13443.0
27	11839.0
28	4690.0
29	1859.0
30	1773.0
31	2063.0
32	2034.0
33	1506.0
34	1118.0
35	1089.0
36	1306.0
37	981.0
38	1051.0
39	3087.0
40	2205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.01669449081803	73.8914858096828	10.689482470784641	9.402337228714524
2	70.37061769616027	5.818030050083473	11.245409015025041	12.56594323873122
3	6.656093489148581	69.81803005008346	10.378964941569283	13.146911519198664
4	6.151919866444073	5.838063439065109	75.74290484140234	12.26711185308848
5	4.814691151919867	6.210350584307179	10.412353923205341	78.56260434056762
6	71.48080133555926	5.9849749582637735	11.312186978297161	11.2220367278798
7	70.95158597662771	7.5893155258764615	8.203672787979967	13.255425709515858
8	7.253756260434057	72.12520868113522	11.699499165275459	8.921535893155259
9	6.57762938230384	73.38397328881469	8.697829716193656	11.340567612687813
10	71.40734557595994	10.297161936560935	8.181969949916528	10.113522537562604
11	7.731218697829716	74.45409015025042	7.834724540901503	9.979966611018364
12	72.63939899833055	9.015025041736227	7.143572621035059	11.202003338898162
13	8.27212020033389	10.08347245409015	7.953255425709516	73.69115191986644
14	73.8914858096828	9.784641068447412	7.616026711185309	8.707846410684473
15	8.078464106844741	9.180300500834724	72.2838063439065	10.457429048414022
16	8.303839732888147	10.853088480801336	7.919866444073456	72.92320534223707
17	74.42237061769616	10.702838063439065	7.484140233722872	7.3906510851419025
18	9.195325542570952	10.130217028380635	71.88146911519199	8.792988313856426
19	74.64774624373956	10.570951585976628	7.292153589315525	7.48914858096828
20	19.163606010016697	11.3889816360601	61.98497495826377	7.462437395659433
21	12.452420701168615	71.43739565943238	7.093489148580969	9.016694490818029
22	74.81135225375625	12.203672787979967	6.527545909849749	6.457429048414023
23	77.01168614357262	10.796327212020033	5.786310517529215	6.405676126878131
24	77.04173622704508	12.003338898163605	5.166944908180301	5.787979966611019
25	17.98330550918197	69.86477462437396	6.629382303839733	5.522537562604341
26	14.445742904841403	74.65776293823039	5.415692821368948	5.480801335559265
27	12.504173622704506	75.07679465776293	6.2938230383973295	6.125208681135225
28	26.741235392320533	61.55926544240401	5.347245409015025	6.352253756260434
29	16.92153589315526	72.18697829716194	6.365609348914858	4.525876460767947
30	53.5008347245409	34.31051752921535	6.59432387312187	5.594323873121869
31	19.128547579298832	9.253756260434058	65.84974958263773	5.767946577629382
32	78.68781302170284	8.035058430717863	8.734557595993323	4.542570951585977
33	13.143572621035057	10.128547579298832	71.52921535893155	5.198664440734558
34	14.061769616026712	11.210350584307179	69.96994991652754	4.757929883138564
35	13.878130217028382	10.76126878130217	15.642737896494157	59.7178631051753
36	14.564273789649414	10.779632721202004	67.21535893155259	7.440734557595993
37	27.46076794657763	8.445742904841403	59.70283806343907	4.3906510851419025
38	14.430717863105174	11.118530884808013	70.60267111853088	3.8480801335559263
39	16.402337228714526	9.67779632721202	7.6427378964941575	66.2771285475793
40	56.694490818030054	13.86143572621035	23.292153589315525	6.151919866444073
41	15.637729549248746	10.88313856427379	69.69949916527545	3.7796327212020033
42	55.751252086811355	12.345575959933223	28.065108514190317	3.8380634390651083
43	14.195325542570952	9.94991652754591	71.98163606010016	3.8731218697829717
44	37.44407345575959	12.35559265442404	45.48080133555927	4.719532554257095
45	13.22704507512521	10.64941569282137	72.0016694490818	4.121869782971619
46	14.929883138564273	13.853088480801334	9.87813021702838	61.33889816360602
47	45.4440734557596	37.87479131886477	11.121869782971618	5.559265442404007
48	10.929883138564273	68.35726210350585	14.253756260434056	6.459098497495826
49	11.011686143572621	6.450751252086811	33.5542570951586	48.98330550918197
50	11.252086811352253	5.547579298831386	14.268781302170282	68.93155258764607
51	11.687813021702837	6.168614357262103	36.489148580968276	45.65442404006678
52	11.479131886477463	7.799666110183639	13.580968280467445	67.14023372287146
53	18.71452420701169	9.736227045075125	18.080133555926544	53.469115191986646
54	64.63272120200334	13.757929883138564	16.185308848080133	5.424040066777963
55	11.662771285475792	8.412353923205343	74.97328881469116	4.951585976627713
56	34.4546653533448	6.666221472812568	53.52343110903354	5.355682064809096
57	68.91485809682804	7.268781302170284	14.652754590984976	9.163606010016695
58	10.823038397328881	5.839732888146912	76.87145242070117	6.465776293823039
59	7.621035058430718	5.873121869782972	21.70450751252087	64.80133555926544
60	25.3414137366857	10.075461618084077	51.228755551103546	13.35436909412668
61	10.347418153892384	7.026828494632631	13.532780179969617	69.09297317150536
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	1.0
17	0.5
18	1.0
19	1.0
20	2.0
21	3.0
22	5.0
23	7.0
24	10.0
25	11.0
26	9.0
27	21.0
28	33.0
29	24.5
30	17.0
31	18.0
32	13.5
33	9.0
34	99.5
35	140.0
36	90.0
37	200.0
38	310.0
39	237.0
40	353.5
41	543.0
42	1110.0
43	2630.5
44	3584.0
45	5068.0
46	6552.0
47	8138.5
48	10878.5
49	12032.0
50	10662.0
51	9292.0
52	8137.0
53	5665.5
54	4349.0
55	3170.5
56	1992.0
57	1421.0
58	620.5
59	391.0
60	310.0
61	213.0
62	197.0
63	168.5
64	140.0
65	125.0
66	121.5
67	133.0
68	92.0
69	51.0
70	61.0
71	56.0
72	41.0
73	29.0
74	17.0
75	13.5
76	11.0
77	12.0
78	7.0
79	2.0
80	1.5
81	2.0
82	3.0
83	3.5
84	2.5
85	1.0
86	1.0
87	1.0
88	1.5
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	1.5
97	3.0
98	2.5
99	18.0
100	34.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.001669449081803005
57	0.0
58	0.0
59	0.0
60	0.00333889816360601
61	0.001669449081803005
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	59900.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.59098497495826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.48294483936738	53.6126878130217
2	5.527084366561966	6.697829716193657
3	2.0609467129553094	3.746243739565943
4	0.9340386840800132	2.263772954924875
5	0.5235025073014823	1.5859766277128546
6	0.43257838761227746	1.5726210350584306
7	0.24797487187964953	1.0517529215358932
8	0.2231773846916846	1.0818030050083474
9	0.20113517385793797	1.0968280467445741
>10	1.1792582796054445	13.869782971619365
>50	0.12674271229404308	5.6928213689482465
>100	0.057860803438584886	6.073455759599333
>500	0.0027552763542183284	1.6544240400667778
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	991	1.6544240400667778	No Hit
AGATCGGAAGAGCCTCGTGTAGGGAAAGAGTGTTCTGTCGTGTGTAGATCTCGGTGGTCGC	363	0.6060100166944908	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTTGTCTC	307	0.5125208681135226	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTTGTCTC	284	0.47412353923205347	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTTTTTCAACCCCCGTTGTCTC	242	0.4040066777963272	Illumina Single End PCR Primer 1 (96% over 29bp)
TATCGTAATGCACGACTACTTAACAGGGGGATTCACCGCAAATACTAGCTTGGCTCATTAT	222	0.3706176961602671	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCAACCCCCGTTGTCTC	218	0.3639398998330551	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCCCCGTTGTCTC	190	0.31719532554257096	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTTGTCGC	155	0.2587646076794658	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTTTTTCAACCCCCGTTGTCTC	154	0.25709515859766274	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTTTTTCAACCCCCGTTGTCTC	150	0.25041736227045075	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCGACCCCCGTTGTCTC	147	0.24540901502504175	Illumina Single End PCR Primer 1 (96% over 29bp)
TGCTGAAGATGGCGGTATATAGGCTGAATTGGCAAGGGTTGGTAAGGTCTATCGGGGTTTA	144	0.2404006677796327	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCGACCCCCGTTGTCTC	136	0.2270450751252087	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTTTTTCGACCCCCGTTGTCTC	126	0.21035058430717862	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTTTTTCGACCCCCGTTGTCTC	124	0.2070116861435726	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTGTTTCGACCCCCGTTGTCTC	122	0.20367278797996663	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCAACCCCCGTTGTCTC	121	0.2020033388981636	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTGTTTCAACCCCCGTTGTCTC	116	0.19365609348914858	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTTGTCGC	111	0.18530884808013354	Illumina Single End PCR Primer 1 (96% over 33bp)
AAGATCTGATGTGGGGATTATATATGAGTCAAAGGTGAGGTCTTCATAGTCTGTATACTCA	104	0.17362270450751252	No Hit
CTTCTTCAAAAAGGTCTAATGGGTAAGCTACATACGCAATAAATTGAGTTTCTTCTCCTGG	102	0.17028380634390652	No Hit
TTCGACTAATGATTCTAACAAAAATCCGTTTTTGGGGTGCAACAAGAGTTTGTATTCTCAA	100	0.1669449081803005	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTTTTTCGACCCCCGTTGTCTC	93	0.15525876460767948	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTTTTTCAACCCCCGTTGTCGC	93	0.15525876460767948	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGATCTCCGTTGTCTC	93	0.15525876460767948	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCAACCCCCGTTGTCTC	92	0.15358931552587646	Illumina Single End PCR Primer 1 (96% over 29bp)
AATCAAAATAGGTGTTGGTACAGGATAGGGTCTCCCCCTCCAGCTGGATCAAAGAAGGTGG	91	0.15191986644407346	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTGTTTCAACCCCCGTTGTCTC	90	0.15025041736227046	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCTCCGTTGTCTC	89	0.14858096828046743	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCGACCCCCGTTGTCTC	89	0.14858096828046743	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTGGTCTC	88	0.14691151919866444	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCAACCCCCGTGGTCTC	87	0.14524207011686144	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTGGTCTC	87	0.14524207011686144	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCCCCGTTGTCGC	86	0.14357262103505844	Illumina Single End PCR Primer 1 (96% over 33bp)
ACCTTAGTTGAACGAAAAGTGCTAGGCTAGATGCAACTCCGTAAAGGACCAAATGTCGTAG	86	0.14357262103505844	No Hit
GGGCATAGTTATTTGGGGAATTGTTGTTGGAGGAATATTGTTGGAAATGATGTATCCTGCG	85	0.1419031719532554	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCCCCGTGGTCTC	85	0.1419031719532554	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTGTTTCGACCCCCGTTGTCTC	84	0.14023372287145242	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCAACCCCCGTTGTCGC	84	0.14023372287145242	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGATCCCCGTTGTCTC	82	0.13689482470784642	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTTTTTCGACCTCCGTTGTCTC	80	0.1335559265442404	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTGGTCGC	77	0.12854757929883137	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCTCCGTTGTCTC	77	0.12854757929883137	Illumina Single End PCR Primer 1 (96% over 33bp)
GAAACCACACTTATCCCCACCTTGGCTATCATCACCCGATGAGGCAACCAGCCAGAACGCC	76	0.1268781302170284	No Hit
TCTATAACTACAGTATTGGACCAAATGAGATTCATTCGTTAGAATAGCTTCCATTGAGTCT	74	0.12353923205342236	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCGACCTCCGTTGTCTC	72	0.12020033388981635	Illumina Single End PCR Primer 1 (96% over 33bp)
TGGCTGGTCGTAGGGGTTCTTTGGTGAATAGTTTTAGGGCATCGGCGATGGGTTGGAGTAG	69	0.11519198664440736	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCGACCCCCGTTGTCTC	69	0.11519198664440736	Illumina Single End PCR Primer 1 (96% over 29bp)
ACATATCAGGGCGGCCAATTAGTAAATTGTCTTGGACCAGTCTAACAGATGATGAGATCGG	69	0.11519198664440736	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTGTTTCGACCTCCGTTGTCTC	68	0.11352253756260434	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCAACCCCCGTGGTCGC	67	0.11185308848080133	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCAACCCCCGTTGTCGC	67	0.11185308848080133	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCGACCCCCGTGGTCTC	65	0.10851419031719532	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTGGTCGC	65	0.10851419031719532	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCAACCCCCGTTGTCGC	65	0.10851419031719532	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCATTGTCTC	62	0.1035058430717863	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCGACCCCCGTTGTCGC	61	0.10183639398998331	Illumina Single End PCR Primer 1 (96% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	63.75626043405676	0.0	0.0	0.0	0.0
2	64.01669449081803	0.0	0.0	0.0	0.0
3	64.10183639398998	0.0	0.0	0.0	0.0
4	64.21869782971619	0.0	0.0	0.0	0.0
5	64.34390651085143	0.0	0.0	0.0	0.0
6	64.69782971619365	0.0	0.0	0.0	0.0
7	65.03338898163607	0.0	0.0	0.0	0.0
8	65.96494156928213	0.0	0.0	0.0	0.0
9	66.6110183639399	0.0	0.0	0.0	0.0
10	67.2253756260434	0.0	0.0	0.0	0.0
11	67.95659432387312	0.0	0.0	0.0	0.0
12	68.75792988313856	0.0	0.0	0.0	0.0
13	69.86811352253756	0.0	0.0	0.0	0.0
14	70.98998330550918	0.0	0.0	0.0	0.0
15	72.52253756260434	0.0	0.0	0.0	0.0
16	74.28881469115191	0.0	0.0	0.0	0.0
17	75.38564273789649	0.0	0.0	0.0	0.0
18	76.87479131886478	0.0	0.0	0.0	0.0
19	77.99332220367279	0.0	0.0	0.0	0.0
20	78.96828046744574	0.0	0.0	0.0	0.0
21	79.97328881469114	0.0	0.0	0.0	0.0
22	80.49248747913188	0.0	0.0	0.0	0.0
23	81.37061769616027	0.0	0.0	0.0	0.0
24	82.32554257095158	0.0	0.0	0.0	0.0
25	82.69449081803005	0.0	0.0	0.0	0.0
26	83.23205342237061	0.0	0.0	0.0	0.0
27	83.66944908180301	0.0	0.0	0.0	0.0
28	84.19365609348915	0.0	0.0	0.0	0.0
29	84.7762938230384	0.0	0.0	0.0	0.0
30	85.42237061769616	0.0	0.0	0.0	0.0
31	86.12854757929883	0.001669449081803005	0.0	0.0	0.0
32	86.75125208681135	0.001669449081803005	0.0	0.0	0.0
33	87.26377295492487	0.005008347245409015	0.0	0.0	0.0
34	87.67946577629382	0.00667779632721202	0.0	0.0	0.0
35	88.08848080133556	0.00667779632721202	0.0	0.0	0.0
36	88.39565943238732	0.00667779632721202	0.0	0.0	0.0
37	88.59766277128547	0.00667779632721202	0.0	0.0	0.0
38	88.75792988313856	0.008347245409015025	0.0	0.0	0.0
39	88.86310517529215	0.008347245409015025	0.0	0.0	0.0
40	88.95826377295492	0.008347245409015025	0.0	0.0	0.0
41	89.09515859766277	0.008347245409015025	0.0	0.0	0.0
42	89.24707846410685	0.008347245409015025	0.0	0.0	0.0
43	89.35726210350585	0.008347245409015025	0.0	0.0	0.0
44	89.54924874791318	0.008347245409015025	0.0	0.0	0.0
45	91.52253756260434	0.008347245409015025	0.0	0.0	0.0
46	91.61936560934892	0.008347245409015025	0.0	0.0	0.0
47	91.68447412353923	0.008347245409015025	0.0	0.0	0.0
48	91.88313856427379	0.008347245409015025	0.0	0.0	0.0
49	92.01168614357262	0.008347245409015025	0.0	0.0	0.0
50	92.0567612687813	0.008347245409015025	0.0	0.0	0.0
>>END_MODULE