FASTQC-Report Basic StatisticsFilenameAM-AL-EC2_S86_R2_001.fastq.gzFile typeConventional base callsEncodingSanger / Illumina 1.9Total Sequences59900Sequences flagged as poor quality0Sequence length61%GC49Per base sequence qualityPer tile sequence qualityPer sequence quality scoresPer base sequence contentPer sequence GC contentPer base N contentSequence Length DistributionSequence Duplication LevelsOverrepresented sequencesATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG9911.6544240400667778No HitAGATCGGAAGAGCCTCGTGTAGGGAAAGAGTGTTCTGTCGTGTGTAGATCTCGGTGGTCGC3630.6060100166944908Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTTGTCTC3070.5125208681135226Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTTGTCTC2840.47412353923205347Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTTTTTCAACCCCCGTTGTCTC2420.4040066777963272Illumina Single End PCR Primer 1 (96% over 29bp)TATCGTAATGCACGACTACTTAACAGGGGGATTCACCGCAAATACTAGCTTGGCTCATTAT2220.3706176961602671No HitAGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCAACCCCCGTTGTCTC2180.3639398998330551Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCCCCGTTGTCTC1900.31719532554257096Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTTGTCGC1550.2587646076794658Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTTTTTCAACCCCCGTTGTCTC1540.25709515859766274Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTTTTTCAACCCCCGTTGTCTC1500.25041736227045075Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCGACCCCCGTTGTCTC1470.24540901502504175Illumina Single End PCR Primer 1 (96% over 29bp)TGCTGAAGATGGCGGTATATAGGCTGAATTGGCAAGGGTTGGTAAGGTCTATCGGGGTTTA1440.2404006677796327No HitAGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCGACCCCCGTTGTCTC1360.2270450751252087Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTTTTTCGACCCCCGTTGTCTC1260.21035058430717862Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTTTTTCGACCCCCGTTGTCTC1240.2070116861435726Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTGTTTCGACCCCCGTTGTCTC1220.20367278797996663Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCAACCCCCGTTGTCTC1210.2020033388981636Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTGTTTCAACCCCCGTTGTCTC1160.19365609348914858Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTTGTCGC1110.18530884808013354Illumina Single End PCR Primer 1 (96% over 33bp)AAGATCTGATGTGGGGATTATATATGAGTCAAAGGTGAGGTCTTCATAGTCTGTATACTCA1040.17362270450751252No HitCTTCTTCAAAAAGGTCTAATGGGTAAGCTACATACGCAATAAATTGAGTTTCTTCTCCTGG1020.17028380634390652No HitTTCGACTAATGATTCTAACAAAAATCCGTTTTTGGGGTGCAACAAGAGTTTGTATTCTCAA1000.1669449081803005No HitAGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTTTTTCGACCCCCGTTGTCTC930.15525876460767948Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTTTTTCAACCCCCGTTGTCGC930.15525876460767948Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGATCTCCGTTGTCTC930.15525876460767948Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCAACCCCCGTTGTCTC920.15358931552587646Illumina Single End PCR Primer 1 (96% over 29bp)AATCAAAATAGGTGTTGGTACAGGATAGGGTCTCCCCCTCCAGCTGGATCAAAGAAGGTGG910.15191986644407346No HitAGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTGTTTCAACCCCCGTTGTCTC900.15025041736227046Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCTCCGTTGTCTC890.14858096828046743Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCGACCCCCGTTGTCTC890.14858096828046743Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTGGTCTC880.14691151919866444Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCAACCCCCGTGGTCTC870.14524207011686144Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTGGTCTC870.14524207011686144Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCCCCGTTGTCGC860.14357262103505844Illumina Single End PCR Primer 1 (96% over 33bp)ACCTTAGTTGAACGAAAAGTGCTAGGCTAGATGCAACTCCGTAAAGGACCAAATGTCGTAG860.14357262103505844No HitGGGCATAGTTATTTGGGGAATTGTTGTTGGAGGAATATTGTTGGAAATGATGTATCCTGCG850.1419031719532554No HitAGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCCCCGTGGTCTC850.1419031719532554Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTGTTTCGACCCCCGTTGTCTC840.14023372287145242Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCAACCCCCGTTGTCGC840.14023372287145242Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGATCCCCGTTGTCTC820.13689482470784642Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTTTTTCGACCTCCGTTGTCTC800.1335559265442404Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTGGTCGC770.12854757929883137Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCTCCGTTGTCTC770.12854757929883137Illumina Single End PCR Primer 1 (96% over 33bp)GAAACCACACTTATCCCCACCTTGGCTATCATCACCCGATGAGGCAACCAGCCAGAACGCC760.1268781302170284No HitTCTATAACTACAGTATTGGACCAAATGAGATTCATTCGTTAGAATAGCTTCCATTGAGTCT740.12353923205342236No HitAGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCGACCTCCGTTGTCTC720.12020033388981635Illumina Single End PCR Primer 1 (96% over 33bp)TGGCTGGTCGTAGGGGTTCTTTGGTGAATAGTTTTAGGGCATCGGCGATGGGTTGGAGTAG690.11519198664440736No HitAGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCGACCCCCGTTGTCTC690.11519198664440736Illumina Single End PCR Primer 1 (96% over 29bp)ACATATCAGGGCGGCCAATTAGTAAATTGTCTTGGACCAGTCTAACAGATGATGAGATCGG690.11519198664440736No HitAGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTGTTTCGACCTCCGTTGTCTC680.11352253756260434Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCAACCCCCGTGGTCGC670.11185308848080133Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCAACCCCCGTTGTCGC670.11185308848080133Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCGACCCCCGTGGTCTC650.10851419031719532Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTGGTCGC650.10851419031719532Illumina Single End PCR Primer 1 (96% over 33bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCAACCCCCGTTGTCGC650.10851419031719532Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCATTGTCTC620.1035058430717863Illumina Single End PCR Primer 1 (96% over 29bp)AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCGACCCCCGTTGTCGC610.10183639398998331Illumina Single End PCR Primer 1 (96% over 29bp)Adapter Content