AM-AL-EC2_S86_R2_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	AM-AL-EC2_S86_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	59900
Sequences flagged as poor quality	0
Sequence length	61
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	991	1.6544240400667778	No Hit
AGATCGGAAGAGCCTCGTGTAGGGAAAGAGTGTTCTGTCGTGTGTAGATCTCGGTGGTCGC	363	0.6060100166944908	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTTGTCTC	307	0.5125208681135226	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTTGTCTC	284	0.47412353923205347	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTTTTTCAACCCCCGTTGTCTC	242	0.4040066777963272	Illumina Single End PCR Primer 1 (96% over 29bp)
TATCGTAATGCACGACTACTTAACAGGGGGATTCACCGCAAATACTAGCTTGGCTCATTAT	222	0.3706176961602671	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCAACCCCCGTTGTCTC	218	0.3639398998330551	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCCCCGTTGTCTC	190	0.31719532554257096	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTTGTCGC	155	0.2587646076794658	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTTTTTCAACCCCCGTTGTCTC	154	0.25709515859766274	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTTTTTCAACCCCCGTTGTCTC	150	0.25041736227045075	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCGACCCCCGTTGTCTC	147	0.24540901502504175	Illumina Single End PCR Primer 1 (96% over 29bp)
TGCTGAAGATGGCGGTATATAGGCTGAATTGGCAAGGGTTGGTAAGGTCTATCGGGGTTTA	144	0.2404006677796327	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCGACCCCCGTTGTCTC	136	0.2270450751252087	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTTTTTCGACCCCCGTTGTCTC	126	0.21035058430717862	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTTTTTCGACCCCCGTTGTCTC	124	0.2070116861435726	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTGTTTCGACCCCCGTTGTCTC	122	0.20367278797996663	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCAACCCCCGTTGTCTC	121	0.2020033388981636	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTGTTTCAACCCCCGTTGTCTC	116	0.19365609348914858	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTTGTCGC	111	0.18530884808013354	Illumina Single End PCR Primer 1 (96% over 33bp)
AAGATCTGATGTGGGGATTATATATGAGTCAAAGGTGAGGTCTTCATAGTCTGTATACTCA	104	0.17362270450751252	No Hit
CTTCTTCAAAAAGGTCTAATGGGTAAGCTACATACGCAATAAATTGAGTTTCTTCTCCTGG	102	0.17028380634390652	No Hit
TTCGACTAATGATTCTAACAAAAATCCGTTTTTGGGGTGCAACAAGAGTTTGTATTCTCAA	100	0.1669449081803005	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTTTTTCGACCCCCGTTGTCTC	93	0.15525876460767948	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTTTTTCAACCCCCGTTGTCGC	93	0.15525876460767948	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGATCTCCGTTGTCTC	93	0.15525876460767948	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCAACCCCCGTTGTCTC	92	0.15358931552587646	Illumina Single End PCR Primer 1 (96% over 29bp)
AATCAAAATAGGTGTTGGTACAGGATAGGGTCTCCCCCTCCAGCTGGATCAAAGAAGGTGG	91	0.15191986644407346	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTGTTTCAACCCCCGTTGTCTC	90	0.15025041736227046	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCTCCGTTGTCTC	89	0.14858096828046743	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCGACCCCCGTTGTCTC	89	0.14858096828046743	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTGGTCTC	88	0.14691151919866444	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCAACCCCCGTGGTCTC	87	0.14524207011686144	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTGGTCTC	87	0.14524207011686144	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCCCCGTTGTCGC	86	0.14357262103505844	Illumina Single End PCR Primer 1 (96% over 33bp)
ACCTTAGTTGAACGAAAAGTGCTAGGCTAGATGCAACTCCGTAAAGGACCAAATGTCGTAG	86	0.14357262103505844	No Hit
GGGCATAGTTATTTGGGGAATTGTTGTTGGAGGAATATTGTTGGAAATGATGTATCCTGCG	85	0.1419031719532554	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGACCCCCGTGGTCTC	85	0.1419031719532554	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCTTGTTTCGACCCCCGTTGTCTC	84	0.14023372287145242	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCAACCCCCGTTGTCGC	84	0.14023372287145242	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCGATCCCCGTTGTCTC	82	0.13689482470784642	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTTTTTCGACCTCCGTTGTCTC	80	0.1335559265442404	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCGTGGTCGC	77	0.12854757929883137	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCTCCGTTGTCTC	77	0.12854757929883137	Illumina Single End PCR Primer 1 (96% over 33bp)
GAAACCACACTTATCCCCACCTTGGCTATCATCACCCGATGAGGCAACCAGCCAGAACGCC	76	0.1268781302170284	No Hit
TCTATAACTACAGTATTGGACCAAATGAGATTCATTCGTTAGAATAGCTTCCATTGAGTCT	74	0.12353923205342236	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTTTTTCGACCTCCGTTGTCTC	72	0.12020033388981635	Illumina Single End PCR Primer 1 (96% over 33bp)
TGGCTGGTCGTAGGGGTTCTTTGGTGAATAGTTTTAGGGCATCGGCGATGGGTTGGAGTAG	69	0.11519198664440736	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCGACCCCCGTTGTCTC	69	0.11519198664440736	Illumina Single End PCR Primer 1 (96% over 29bp)
ACATATCAGGGCGGCCAATTAGTAAATTGTCTTGGACCAGTCTAACAGATGATGAGATCGG	69	0.11519198664440736	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCTTGTTTCGACCTCCGTTGTCTC	68	0.11352253756260434	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCAACCCCCGTGGTCGC	67	0.11185308848080133	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCAACCCCCGTTGTCGC	67	0.11185308848080133	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTGTCGACCCCCGTGGTCTC	65	0.10851419031719532	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTCTTTCGTGTTTCAACCCCCGTGGTCGC	65	0.10851419031719532	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTGTCAACCCCCGTTGTCGC	65	0.10851419031719532	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCAACCCCCATTGTCTC	62	0.1035058430717863	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTTCGTGTTTCGACCCCCGTTGTCGC	61	0.10183639398998331	Illumina Single End PCR Primer 1 (96% over 29bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content