##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-EC2_S86_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	59900
Sequences flagged as poor quality	0
Sequence length	61
%GC	58
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.136727879799665	40.0	40.0	40.0	40.0	40.0
2	38.96400667779633	40.0	40.0	40.0	40.0	40.0
3	38.68153589315526	40.0	40.0	40.0	40.0	40.0
4	39.02677796327212	40.0	40.0	40.0	40.0	40.0
5	38.978497495826375	40.0	40.0	40.0	40.0	40.0
6	38.84500834724541	40.0	40.0	40.0	40.0	40.0
7	39.05742904841402	40.0	40.0	40.0	40.0	40.0
8	38.794457429048414	40.0	40.0	40.0	40.0	40.0
9	39.100767946577626	40.0	40.0	40.0	40.0	40.0
10	39.16500834724541	40.0	40.0	40.0	40.0	40.0
11	39.243405676126876	40.0	40.0	40.0	40.0	40.0
12	39.276327212020036	40.0	40.0	40.0	40.0	40.0
13	38.995993322203674	40.0	40.0	40.0	40.0	40.0
14	39.20854757929883	40.0	40.0	40.0	40.0	40.0
15	39.412353923205345	40.0	40.0	40.0	40.0	40.0
16	39.33048414023372	40.0	40.0	40.0	40.0	40.0
17	39.372754590984975	40.0	40.0	40.0	40.0	40.0
18	39.33669449081803	40.0	40.0	40.0	40.0	40.0
19	39.32767946577629	40.0	40.0	40.0	40.0	40.0
20	33.90110183639399	40.0	24.0	40.0	24.0	40.0
21	39.454090150250416	40.0	40.0	40.0	40.0	40.0
22	39.21969949916527	40.0	40.0	40.0	40.0	40.0
23	39.25375626043406	40.0	40.0	40.0	40.0	40.0
24	39.28787979966611	40.0	40.0	40.0	40.0	40.0
25	39.54878130217028	40.0	40.0	40.0	40.0	40.0
26	39.44874791318865	40.0	40.0	40.0	40.0	40.0
27	39.30450751252087	40.0	40.0	40.0	40.0	40.0
28	39.30524207011686	40.0	40.0	40.0	40.0	40.0
29	39.2134223706177	40.0	40.0	40.0	40.0	40.0
30	39.196460767946576	40.0	40.0	40.0	40.0	40.0
31	39.298497495826375	40.0	40.0	40.0	40.0	40.0
32	39.36774624373957	40.0	40.0	40.0	40.0	40.0
33	39.11278797996661	40.0	40.0	40.0	40.0	40.0
34	39.28420701168614	40.0	40.0	40.0	40.0	40.0
35	39.21669449081803	40.0	40.0	40.0	40.0	40.0
36	39.170150250417365	40.0	40.0	40.0	40.0	40.0
37	39.07813021702838	40.0	40.0	40.0	40.0	40.0
38	39.117662771285474	40.0	40.0	40.0	40.0	40.0
39	39.086410684474124	40.0	40.0	40.0	40.0	40.0
40	37.088881469115194	40.0	40.0	40.0	24.0	40.0
41	39.18998330550918	40.0	40.0	40.0	40.0	40.0
42	39.10824707846411	40.0	40.0	40.0	40.0	40.0
43	38.90964941569282	40.0	40.0	40.0	40.0	40.0
44	38.72741235392321	40.0	40.0	40.0	40.0	40.0
45	28.799532554257095	24.0	24.0	40.0	12.0	40.0
46	32.66357262103506	40.0	24.0	40.0	24.0	40.0
47	36.38797996661102	40.0	40.0	40.0	24.0	40.0
48	38.323405676126875	40.0	40.0	40.0	40.0	40.0
49	38.1065776293823	40.0	40.0	40.0	40.0	40.0
50	37.67091819699499	40.0	40.0	40.0	24.0	40.0
51	36.74023372287145	40.0	40.0	40.0	24.0	40.0
52	36.341235392320534	40.0	40.0	40.0	24.0	40.0
53	34.45422370617696	40.0	24.0	40.0	24.0	40.0
54	30.946577629382304	40.0	24.0	40.0	12.0	40.0
55	30.544106844741236	40.0	24.0	40.0	12.0	40.0
56	29.933288814691153	24.0	24.0	40.0	12.0	40.0
57	27.960467445742903	24.0	24.0	40.0	12.0	40.0
58	26.042337228714523	24.0	12.0	40.0	12.0	40.0
59	23.886410684474125	24.0	12.0	40.0	12.0	40.0
60	23.064774624373957	24.0	12.0	40.0	12.0	40.0
61	22.5612020033389	12.0	12.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5326885042731462
1101	2	0.6492119367111897
1101	3	0.9631306147687511
1101	4	0.26347629690270225
1101	5	0.6235691651992497
1101	6	0.0039514850468265195
1101	7	0.12977369066243227
1101	8	0.3454147238321923
1101	9	0.09278412511325485
1101	10	0.21989102327745513
1101	11	0.3080259034798587
1101	12	0.16253099065563248
1101	13	0.14557503320841647
1101	14	0.2658105711198431
1101	15	0.21155422672133994
1101	16	-0.13637423754130396
1101	17	0.21763233186855757
1101	18	0.2813228841002271
1101	19	0.14731906248597681
1101	20	0.579223185087784
1101	21	0.1533832076600774
1101	22	-0.08281443977374181
1101	23	0.07995857400022288
1101	24	0.2761870670470188
1101	25	0.09645491450670107
1101	26	0.22047215377272522
1101	27	0.25950568660619666
1101	28	0.1204983701110649
1101	29	0.40491878641324064
1101	30	-0.10017525331331711
1101	31	-0.20423484085493016
1101	32	-0.02688313950917376
1101	33	0.1523289266183383
1101	34	0.17721076348743026
1101	35	0.13386285802698694
1101	36	0.012313394758258767
1101	37	0.23621976984142634
1101	38	0.3435630608961162
1101	39	-0.28776928224133513
1101	40	0.8022635948695935
1101	41	0.14319773999002194
1101	42	0.22052841549312063
1101	43	-0.21496373945565495
1101	44	0.29673149921436703
1101	45	-0.587526379472024
1101	46	0.5388651491133203
1101	47	1.251075941191388
1101	48	1.0037544799882596
1101	49	0.5579432024559026
1101	50	0.6932740694731407
1101	51	0.9690829764517872
1101	52	0.4887130720590278
1101	53	0.8882980028387948
1101	54	0.6325876524771026
1101	55	-1.0492103102208716
1101	56	-0.4239607145780191
1101	57	-0.5840132034324625
1101	58	0.22392364755093652
1101	59	-0.9524930483003651
1101	60	-0.4766292775691028
1101	61	0.07591262129313847
1102	1	0.24741571216569724
1102	2	0.237824797759572
1102	3	-0.19685987613817701
1102	4	0.04833306618832722
1102	5	-0.4725915384736368
1102	6	0.09725946079863945
1102	7	-0.11199500064863344
1102	8	0.3930790528468151
1102	9	0.1372391352166673
1102	10	-0.08356790932685243
1102	11	0.03761106929466962
1102	12	-0.31328025384692637
1102	13	0.2815550641129718
1102	14	0.2624823885452585
1102	15	-0.05458151558223534
1102	16	-0.042947398126109704
1102	17	0.014256603828620484
1102	18	0.25992742469217234
1102	19	0.2120997590270477
1102	20	0.92143167195335
1102	21	-0.02443683275356534
1102	22	0.34259861145650206
1102	23	0.20428996589372161
1102	24	0.4559089260747129
1102	25	0.079338546980253
1102	26	-0.13076997150957226
1102	27	0.1934174897679739
1102	28	0.24482976200456363
1102	29	0.049397569249329365
1102	30	0.10177411076608678
1102	31	-0.02463184549061026
1102	32	0.29428647893846716
1102	33	-0.2192388851016247
1102	34	-0.29753070804472515
1102	35	-0.07914082674658118
1102	36	-0.22731575061200004
1102	37	-0.2726355730802368
1102	38	0.22636348880530477
1102	39	0.1907758265189159
1102	40	0.21424505213811074
1102	41	-0.18260756132936962
1102	42	-0.28083851663573256
1102	43	0.5678723475525516
1102	44	0.4082256154630315
1102	45	-0.24805175686415382
1102	46	0.3209880541412531
1102	47	-0.7915961139613472
1102	48	-0.21697534290488818
1102	49	-0.02555852106721801
1102	50	-0.2840229708216455
1102	51	-0.48937417319756094
1102	52	-0.31765802029804036
1102	53	-0.3973313802587981
1102	54	-0.8636681421264853
1102	55	-0.38274174965983576
1102	56	-3.034919944341482
1102	57	-1.5472843314486262
1102	58	-2.0431630040596644
1102	59	-0.7730089165994265
1102	60	-1.349682885081286
1102	61	-1.3972836226150989
1103	1	0.6218051356644807
1103	2	0.15294369565070554
1103	3	0.11084143129988888
1103	4	0.4214355260438367
1103	5	0.10736107786495097
1103	6	0.5053439324123161
1103	7	0.686975428436746
1103	8	0.0024270887647972472
1103	9	0.588495387227546
1103	10	0.5031705153126538
1103	11	0.3157122129373633
1103	12	0.48569017523845304
1103	13	0.1589425279171195
1103	14	0.043464575798466853
1103	15	0.44827027885457227
1103	16	-0.18650234269892962
1103	17	0.45568513347265593
1103	18	0.3089490398315391
1103	19	0.07123573843562525
1103	20	-0.3583951729382875
1103	21	-0.09124194550913245
1103	22	0.4125626349728364
1103	23	0.5507171793249412
1103	24	0.5341797149249317
1103	25	0.038974687259290874
1103	26	0.37208182292723535
1103	27	-0.11077391682479032
1103	28	0.1305239911425886
1103	29	0.4112683463998721
1103	30	0.29048977954844446
1103	31	0.10266055849897526
1103	32	0.1310760896319607
1103	33	0.14274888874376757
1103	34	-0.21200612411421815
1103	35	-0.2952337221222834
1103	36	0.14999859025787288
1103	37	0.24858470244697628
1103	38	0.059058215179284446
1103	39	0.5260883539400183
1103	40	-0.45907092668549154
1103	41	-0.1599102025297512
1103	42	0.3143236700325005
1103	43	0.11989200299728253
1103	44	0.5830186775590249
1103	45	-0.6516903540737822
1103	46	-0.5030196676406078
1103	47	0.30454592002618597
1103	48	0.7847503583440556
1103	49	0.5377805646036151
1103	50	0.5518014171393943
1103	51	0.7964195031310837
1103	52	0.5800576192351414
1103	53	1.3387825745219857
1103	54	1.6863918186796205
1103	55	-0.12651898795285277
1103	56	-0.04811132168507015
1103	57	-1.0300419435460846
1103	58	-0.645520487460761
1103	59	-0.9527158398788451
1103	60	-1.306862094768615
1103	61	-1.383595009318423
1104	1	0.240460974963554
1104	2	0.2371407252478832
1104	3	-0.2040426375108808
1104	4	0.048105042017766664
1104	5	-0.19406001413205587
1104	6	-0.02644365173128449
1104	7	-0.20457281389683857
1104	8	-0.29418579722729277
1104	9	0.37415624843066553
1104	10	0.003651335913147591
1104	11	0.14432638111772178
1104	12	0.24720315739466514
1104	13	0.42521029156708323
1104	14	0.47032642001259006
1104	15	-0.32296596433380387
1104	16	0.3660139517769778
1104	17	0.38046342175132253
1104	18	0.07420173776935712
1104	19	0.3917828054299619
1104	20	-0.8437034323677111
1104	21	0.027894714390427566
1104	22	-0.2308821775071337
1104	23	0.0651952218508498
1104	24	0.2323312268385962
1104	25	0.13075799744200367
1104	26	0.3763557838204932
1104	27	0.37891515252022145
1104	28	-0.17997926775259288
1104	29	0.25815369739891025
1104	30	0.24155292732064026
1104	31	0.05586068671779998
1104	32	0.32917417703446006
1104	33	-0.06304232826659728
1104	34	0.40387164060423686
1104	35	-0.430184037326903
1104	36	-0.09928017884138995
1104	37	-0.24185231005435526
1104	38	-0.123653613007491
1104	39	0.3305546430734694
1104	40	0.5517208245699834
1104	41	0.29430499140121924
1104	42	0.022091593955991584
1104	43	0.532756625285991
1104	44	0.27198117355219154
1104	45	0.04165295183496909
1104	46	-0.5019511774172933
1104	47	-0.24262792333314565
1104	48	0.19654649040944605
1104	49	0.34908519116628156
1104	50	0.04672608857865157
1104	51	0.3299166716319917
1104	52	0.760098221863629
1104	53	0.5079245768726608
1104	54	0.4662828326768427
1104	55	0.6138378877817345
1104	56	0.15137580300773834
1104	57	-0.5662103376052805
1104	58	0.3250960313980933
1104	59	-0.49048696927301094
1104	60	-0.21913904743449564
1104	61	-0.6487942827450723
1201	1	0.18037660602044525
1201	2	0.13726613854169045
1201	3	0.09364291715791495
1201	4	-0.3985943081133456
1201	5	0.577687791135304
1201	6	0.20899130437405944
1201	7	-0.3354586877994663
1201	8	-0.09854105888502573
1201	9	-0.28734187036992154
1201	10	0.07285667167873555
1201	11	-0.18585261785617035
1201	12	-0.38031935999217836
1201	13	0.03574500824704785
1201	14	0.15075054496983853
1201	15	0.10184306542335264
1201	16	0.09113081416439428
1201	17	-0.3209389268976395
1201	18	-0.1646535808944236
1201	19	0.10036791545162771
1201	20	0.429811560221502
1201	21	0.3331050666877715
1201	22	0.09678855559057808
1201	23	0.09255812231830163
1201	24	-0.3709067163833879
1201	25	-0.25585698374599986
1201	26	-0.06373086536432027
1201	27	0.4168811769188068
1201	28	-0.11271213743678032
1201	29	0.049397569249329365
1201	30	0.034735004620834786
1201	31	0.06475362936972573
1201	32	0.18255463536304717
1201	33	-0.2192388851016247
1201	34	-0.051720652178801174
1201	35	-0.07914082674658118
1201	36	0.06318704268409192
1201	37	0.040213588930932076
1201	38	0.29340259495055676
1201	39	0.2578149326641679
1201	40	-0.18798958473340122
1201	41	-0.0038366116086976376
1201	42	0.32251343867153537
1201	43	-0.08017234518488436
1201	44	0.7657675149043754
1201	45	-1.633526617199351
1201	46	-0.03655384530009087
1201	47	-0.009473208933414412
1201	48	0.40872298111746375
1201	49	0.13086605993836997
1201	50	-0.6639112389780664
1201	51	-0.17652501118639208
1201	52	0.7996604154561524
1201	53	-0.866605123275555
1201	54	0.25365029362770386
1201	55	1.2485431665412818
1201	56	1.2332364802395226
1201	57	0.8437771210653402
1201	58	0.7724794540408944
1201	59	1.685091642059792
1201	60	0.37098750598016395
1201	61	0.7703141427480311
1202	1	0.22664938972339854
1202	2	0.6898308922237604
1202	3	0.23607280260456065
1202	4	-0.10402853868724549
1202	5	-0.4841033041753491
1202	6	0.3299777046900729
1202	7	0.8574762481522242
1202	8	0.38732316999595895
1202	9	0.380340540329243
1202	10	0.23514999598323527
1202	11	0.3933472015671171
1202	12	0.27235261111555076
1202	13	0.1077499741067669
1202	14	0.21079230333561583
1202	15	0.21176914182987616
1202	16	0.14688387550404514
1202	17	0.10093342793560822
1202	18	-2.1590807686067137E-4
1202	19	-0.1714000434671732
1202	20	1.490248330155481
1202	21	0.11426865790723184
1202	22	-0.18017098393879394
1202	23	0.07179179987600293
1202	24	-0.14676586654421442
1202	25	-0.007563998022845908
1202	26	-0.1674496171277653
1202	27	0.018370934833150443
1202	28	0.011321494976741064
1202	29	-0.019108722720652338
1202	30	-0.07631967626625169
1202	31	-0.13997522340382318
1202	32	0.15106657035542526
1202	33	-0.11416580796150555
1202	34	0.043533565980446554
1202	35	-0.013568906426058902
1202	36	0.050998114294046104
1202	37	0.17496896390976957
1202	38	-0.15375050024723436
1202	39	0.15409618090072286
1202	40	0.8003293588928031
1202	41	0.4008809551200443
1202	42	0.2527656817729351
1202	43	0.2759023684317299
1202	44	0.07923740937985002
1202	45	-0.3696024594204417
1202	46	0.6499762602244346
1202	47	0.039169644178706164
1202	48	-0.19203318388451152
1202	49	0.6479926329308654
1202	50	0.13062631533602342
1202	51	0.23880143765961037
1202	52	0.7891643937869404
1202	53	0.9226844202039359
1202	54	0.45205896131011514
1202	55	0.24092508629750498
1202	56	-0.5150847205956453
1202	57	-0.8195601623836772
1202	58	1.556182405341609
1202	59	1.5383730595870198
1202	60	0.9278409566883639
1202	61	1.8609975127610099
1203	1	0.2955739292361699
1203	2	-0.1871744553698207
1203	3	-0.3010632639432771
1203	4	0.002923332970510728
1203	5	-0.29872005996857354
1203	6	-0.6444726815250235
1203	7	-0.28096700793808793
1203	8	0.2126351129549633
1203	9	-0.01894644719303784
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2403	35	-0.08146094153120487
2403	36	-0.11790402182120374
2403	37	-0.06944236246877011
2403	38	0.45959608031246546
2403	39	0.09613956556705716
2403	40	-0.07100695506645849
2403	41	-0.050849463823489316
2403	42	0.3560940473963825
2403	43	-0.16357436612697285
2403	44	0.4034632745893276
2403	45	0.48583718290444367
2403	46	-1.0950146323074463
2403	47	0.9668066701763891
2403	48	0.08781447301232248
2403	49	0.5420148220350214
2403	50	0.5604988107822919
2403	51	0.9051369236672286
2403	52	1.1021301165887323
2403	53	-0.9007962885226775
2403	54	-0.9528093943779119
2403	55	-0.0750212624357296
2403	56	-0.8664673998471741
2403	57	0.5925941678034405
2403	58	-1.185331090270246
2403	59	-1.6224151503815207
2403	60	-1.7063700053914488
2403	61	-1.4143792052386601
2404	1	-0.8819880046282833
2404	2	-0.3546960094459948
2404	3	-0.877512025265986
2404	4	-0.4008745498189654
2404	5	-0.35732532025450325
2404	6	-0.6386885496904711
2404	7	-0.3270217934886688
2404	8	-0.6615327360028047
2404	9	0.04762563618577076
2404	10	-0.11879764367868972
2404	11	-0.059755251535335674
2404	12	-0.5487152099522703
2404	13	-0.08499379006557461
2404	14	0.08257131797177664
2404	15	0.0035646479110909013
2404	16	-0.3278635992434289
2404	17	-0.1501488231466368
2404	18	-0.5176349969245209
2404	19	-0.13882943946799742
2404	20	-0.43554016706158905
2404	21	-0.19659508152793848
2404	22	0.17728108779898832
2404	23	-0.3837843699858823
2404	24	-0.13501571193691575
2404	25	-0.0529154719457523
2404	26	0.2130904776980458
2404	27	-0.09047260258181922
2404	28	0.02410236490046458
2404	29	0.17652104433769011
2404	30	-0.2278348277814004
2404	31	0.36198313569739327
2404	32	0.02305172805486677
2404	33	-0.06304232826659728
2404	34	-0.04510795123250233
2404	35	0.059611881040446235
2404	36	-0.0380556890454713
2404	37	-0.38470945291150116
2404	38	-0.02161279668095517
2404	39	0.3101464798081608
2404	40	-0.6319526448177655
2404	41	0.2126723383399991
2404	42	0.3078058796702763
2404	43	0.0021443803880316636
2404	44	0.27198117355219154
2404	45	-0.28487766040992923
2404	46	0.9062120878888322
2404	47	-0.5283422090474303
2404	48	0.07409751081760874
2404	49	-0.8549964414867759
2404	50	-0.07572289101318574
2404	51	-1.0578384304088289
2404	52	0.2703023034962797
2404	53	-0.28799379047427465
2404	54	-0.6765743101802997
2404	55	-0.7126927244631638
2404	56	-1.8282160337269566
2404	57	-1.9947817661767075
2404	58	-3.0626590706427237
2404	59	-1.8578339080485193
2404	60	-2.6273023127406177
2404	61	-2.7916514256022147
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	9.0
18	19.0
19	15.0
20	27.0
21	28.0
22	54.0
23	83.0
24	106.0
25	152.0
26	223.0
27	282.0
28	393.0
29	444.0
30	580.0
31	765.0
32	1064.0
33	1304.0
34	2109.0
35	5079.0
36	15670.0
37	12900.0
38	4627.0
39	10924.0
40	3040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.584450491660963	73.26666555368203	10.310689660929231	7.838194293727775
2	76.11120387376857	6.852563032225747	8.024711971948573	9.011521122057104
3	8.432387312186979	74.13188647746244	10.267111853088482	7.168614357262104
4	7.819699499165275	7.858096828046744	74.55926544240401	9.762938230383973
5	8.881469115191987	7.6661101836393994	9.560934891485811	73.8914858096828
6	75.44908180300501	7.83973288814691	8.140233722871452	8.570951585976626
7	75.89816360601002	8.808013355592655	8.879799666110182	6.4140233722871445
8	7.248747913188648	83.08848080133556	3.5676126878130217	6.095158597662771
9	15.56093489148581	73.64273789649415	7.10016694490818	3.6961602671118534
10	72.91819699499166	16.514190317195325	6.696160267111853	3.8714524207011687
11	6.956594323873122	72.23873121869782	16.118530884808013	4.6861435726210345
12	73.18030050083473	6.173622704507512	6.632721202003339	14.013355592654426
13	16.93322203672788	6.582637729549249	3.5058430717863103	72.97829716193655
14	16.3906510851419	73.67111853088481	3.1652754590984973	6.772954924874791
15	7.764607679465777	17.465776293823037	4.771285475792989	69.9983305509182
16	7.432387312186978	81.75125208681135	3.9465776293823036	6.869782971619366
17	14.580968280467447	9.397328881469114	4.188647746243739	71.8330550918197
18	73.47245409015025	14.834724540901503	6.5959933222036735	5.096828046744574
19	13.245409015025041	7.590984974958263	70.0	9.163606010016695
20	9.98330550918197	7.367278797996661	4.165275459098497	78.48414023372288
21	11.797996661101836	9.10517529215359	72.47579298831386	6.621035058430717
22	73.98497495826378	8.767946577629381	7.472454090150251	9.774624373956595
23	9.455759599332222	76.06176961602671	4.425709515859767	10.056761268781301
24	13.28881469115192	74.18697829716193	5.64440734557596	6.879799666110184
25	11.095158597662772	9.936560934891487	6.954924874791319	72.01335559265442
26	9.903171953255425	11.175292153589316	72.26043405676127	6.66110183639399
27	12.17195325542571	10.59432387312187	4.409015025041736	72.82470784641069
28	9.168614357262102	11.52754590984975	6.759599332220367	72.54424040066777
29	11.202003338898162	74.80968280467447	5.976627712854758	8.011686143572621
30	75.26377295492487	11.402337228714524	4.996661101836394	8.337228714524207
31	7.749582637729549	12.704507512520868	71.29382303839733	8.252086811352253
32	8.87312186978297	10.050083472454089	6.943238731218698	74.13355592654423
33	9.415692821368948	74.48747913188647	6.258764607679466	9.83806343906511
34	7.48914858096828	9.50584307178631	8.881469115191987	74.12353923205343
35	8.545909849749583	8.595993322203672	7.527545909849749	75.33055091819699
36	73.65609348914857	9.622704507512521	6.786310517529214	9.934891485809683
37	8.333889816360601	12.554257095158597	5.530884808013355	73.58096828046745
38	8.53923205342237	75.92821368948248	6.831385642737898	8.701168614357261
39	7.330550918196995	75.72954924874792	6.465776293823039	10.474123539232053
40	8.901502504173623	9.874791318864775	6.3572621035058425	74.86644407345577
41	6.871452420701169	11.829716193656093	71.56260434056762	9.736227045075125
42	7.889816360601001	74.36727879799666	5.801335559265442	11.941569282136895
43	6.963272120200334	74.87979966611019	7.063439065108514	11.093489148580968
44	6.37228714524207	9.424040066777964	71.3405676126878	12.863105175292153
45	6.702838063439065	11.721202003338899	7.818030050083473	73.75792988313856
46	9.340567612687813	10.086811352253756	68.53923205342237	12.03338898163606
47	70.2787979966611	11.190317195325543	5.76126878130217	12.769616026711187
48	70.73956594323873	9.135225375626044	8.475792988313856	11.64941569282137
49	71.2804674457429	8.74958263772955	5.833055091819699	14.136894824707847
50	72.87646076794658	9.624373956594324	4.816360601001669	12.68280467445743
51	73.23873121869782	9.636060100166945	6.156928213689483	10.968280467445743
52	71.19031719532555	11.63939899833055	5.587646076794658	11.582637729549248
53	71.21869782971619	11.87145242070117	7.131886477462437	9.7779632721202
54	72.91819699499166	9.363939899833056	6.82804674457429	10.889816360601001
55	71.1686143572621	12.253756260434056	6.362270450751252	10.215358931552586
56	73.59098497495826	10.342237061769616	7.3973288814691145	8.669449081803005
57	71.6043405676127	9.520868113522537	6.813021702838064	12.061769616026712
58	71.9415692821369	10.717863105175292	8.868113522537563	8.47245409015025
59	71.47078464106845	8.19365609348915	9.858096828046744	10.47746243739566
60	70.23038397328881	9.370617696160268	11.39398998330551	9.00500834724541
61	68.55926544240401	7.205342237061769	15.637729549248746	8.597662771285476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.5
18	1.0
19	1.0
20	2.0
21	3.0
22	5.5
23	8.0
24	8.0
25	10.5
26	13.0
27	30.0
28	47.0
29	27.5
30	9.0
31	10.0
32	25.5
33	41.0
34	64.5
35	70.0
36	52.0
37	126.5
38	201.0
39	173.5
40	136.5
41	127.0
42	158.0
43	363.5
44	538.0
45	526.5
46	515.0
47	1230.5
48	2107.0
49	2268.0
50	2699.0
51	3130.0
52	3360.0
53	3320.0
54	3050.0
55	2631.0
56	2212.0
57	2029.5
58	2316.5
59	2786.0
60	4772.5
61	17459.0
62	28159.0
63	14872.0
64	1585.0
65	899.5
66	163.5
67	113.0
68	95.0
69	77.0
70	65.0
71	48.5
72	44.0
73	30.0
74	16.0
75	18.5
76	16.5
77	12.0
78	10.0
79	8.0
80	4.5
81	3.5
82	6.0
83	5.0
84	2.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001669449081803005
2	0.01669449081803005
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	59900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.9449081803005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.46861924686192	15.903171953255427
2	17.01027006466337	7.465776293823039
3	5.195891974134652	3.4207011686143574
4	1.909471281856219	1.6761268781302172
5	0.7911753518448078	0.8681135225375626
6	0.36515785469760365	0.4808013355592654
7	0.2586534804108026	0.39732888146911516
8	0.19779383796120195	0.34724540901502504
9	0.14454165081780143	0.2854757929883139
>10	1.3313046785850133	6.171953255425709
>50	0.15214910612400154	2.5926544240400666
>100	0.1293267402054013	4.273789649415693
>500	0.015214910612400151	2.4641068447412353
>1k	0.022822365918600228	8.48247078464107
>5k	0.0	0.0
>10k+	0.007607455306200076	45.17028380634391
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGG	27057	45.17028380634391	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGT	2903	4.846410684474123	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGTG	1177	1.964941569282137	TruSeq Adapter, Index 22 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC	1001	1.671118530884808	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGTT	748	1.2487479131886476	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTGG	728	1.2153589315525877	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTGT	373	0.6227045075125208	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTATCTGGGGGGGGGGGGGGGG	339	0.5659432387312188	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGTGGG	190	0.31719532554257096	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTTT	170	0.2838063439065108	TruSeq Adapter, Index 22 (97% over 37bp)
CTTGGCGGTGCTTCACATCCACCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGATAGA	150	0.25041736227045075	No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGG	142	0.23706176961602674	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATTTGGGGGGGGGGGGGGG	136	0.2270450751252087	TruSeq Adapter, Index 22 (97% over 37bp)
TGAAGAAGTAAACCATTATCTCGGCAATAATGAGCCAAGCTAGTATTTGCGGTGAATCCCC	118	0.1969949916527546	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTTG	110	0.18363939899833057	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGG	109	0.18196994991652754	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGG	107	0.17863105175292154	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCT	105	0.17529215358931552	TruSeq Adapter, Index 22 (97% over 37bp)
GGACATCAATGATACTGAAGCTATGAGTATACAGACTATGAAGACCTCACCTTTGACTCAT	104	0.17362270450751252	No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGG	103	0.17195325542570952	TruSeq Adapter, Index 22 (97% over 37bp)
CGGAATTTCCACAATACATGCAAACCCCTCTGATATCATTTGAGAATACAAACTCTTGTTG	102	0.17028380634390652	No Hit
TTACAAAGGACGATGCTACCACATCGAGCCCGTTCCAGGAGAAGAAACTCAATTTATTGCG	101	0.1686143572621035	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATGTGGGGGGGGGGGGGGG	101	0.1686143572621035	TruSeq Adapter, Index 22 (97% over 37bp)
TGAAGAAGTAAACCATTATCCCGGCAATAATGAGCCAAGCTAGTATTTGCGGTGAATCCCC	100	0.1669449081803005	No Hit
GGTTAGCCTTTCTCAGACCGCAACCTTAATACCACCTTCTTTGATCCAGCTGGAGGGGGAG	98	0.1636060100166945	No Hit
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGG	97	0.16193656093489148	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGGG	88	0.14691151919866444	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCGGGGGGGGGGGGGGGG	86	0.14357262103505844	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTG	85	0.1419031719532554	TruSeq Adapter, Index 22 (97% over 37bp)
AGGGCTAGTGTGAGGGCTAGGTTTGGTGCTATAATGAATATTAATATGGAGGATGTGAGGG	85	0.1419031719532554	No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGG	82	0.13689482470784642	TruSeq Adapter, Index 22 (97% over 37bp)
ACGCTTACTAATTGGAAGCCTCTTCGCAGGATACATCATTTCCAACAATATTCCTCCAACA	79	0.1318864774624374	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGTGGGG	79	0.1318864774624374	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGG	77	0.12854757929883137	TruSeq Adapter, Index 22 (97% over 37bp)
AGACTCAATGGAAGCTATTCTAACGAATGAATCTCATTTGGTCCAATACTGTAGTTATAGA	77	0.12854757929883137	No Hit
AGTAGGGGAAGGGAGCCTACTAGGGTGTAGAATAGGAAGTATGTGCCTGCGTTCAGGCGTT	71	0.11853088480801337	No Hit
CATCATCTGTTAGACTGGTCCAAGACAATTTACTAATTGGCCGCCCTGATATGTAGATCGG	71	0.11853088480801337	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGTGTG	70	0.11686143572621034	TruSeq Adapter, Index 22 (97% over 37bp)
CCTACGGCCTACTCCAACCCATCGCCGATGCCCTAAAACTATTCACCAAAGAACCCCTACG	70	0.11686143572621034	No Hit
AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGG	63	0.10517529215358931	TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGG	62	0.1035058430717863	TruSeq Adapter, Index 22 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	64.54424040066777	0.0	0.0	0.0	0.0
2	64.85308848080133	0.0	0.0	0.0	0.0
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50	91.44741235392321	0.0	0.0	0.0	0.0
>>END_MODULE