FASTQC-Report Basic StatisticsFilenameAM-AL-EC2_S86_R1_001.fastq.gzFile typeConventional base callsEncodingSanger / Illumina 1.9Total Sequences59900Sequences flagged as poor quality0Sequence length61%GC58Per base sequence qualityPer tile sequence qualityPer sequence quality scoresPer base sequence contentPer sequence GC contentPer base N contentSequence Length DistributionSequence Duplication LevelsOverrepresented sequencesAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGG2705745.17028380634391TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGT29034.846410684474123TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGTG11771.964941569282137TruSeq Adapter, Index 22 (97% over 37bp)TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC10011.671118530884808No HitAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGTT7481.2487479131886476TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTGG7281.2153589315525877TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTGT3730.6227045075125208TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTATCTGGGGGGGGGGGGGGGG3390.5659432387312188TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGTGGG1900.31719532554257096TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTTT1700.2838063439065108TruSeq Adapter, Index 22 (97% over 37bp)CTTGGCGGTGCTTCACATCCACCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGATAGA1500.25041736227045075No HitCGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGG1420.23706176961602674TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATTTGGGGGGGGGGGGGGG1360.2270450751252087TruSeq Adapter, Index 22 (97% over 37bp)TGAAGAAGTAAACCATTATCTCGGCAATAATGAGCCAAGCTAGTATTTGCGGTGAATCCCC1180.1969949916527546No HitAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTTG1100.18363939899833057TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGG1090.18196994991652754TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGG1070.17863105175292154TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCT1050.17529215358931552TruSeq Adapter, Index 22 (97% over 37bp)GGACATCAATGATACTGAAGCTATGAGTATACAGACTATGAAGACCTCACCTTTGACTCAT1040.17362270450751252No HitGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGG1030.17195325542570952TruSeq Adapter, Index 22 (97% over 37bp)CGGAATTTCCACAATACATGCAAACCCCTCTGATATCATTTGAGAATACAAACTCTTGTTG1020.17028380634390652No HitTTACAAAGGACGATGCTACCACATCGAGCCCGTTCCAGGAGAAGAAACTCAATTTATTGCG1010.1686143572621035No HitAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATGTGGGGGGGGGGGGGGG1010.1686143572621035TruSeq Adapter, Index 22 (97% over 37bp)TGAAGAAGTAAACCATTATCCCGGCAATAATGAGCCAAGCTAGTATTTGCGGTGAATCCCC1000.1669449081803005No HitGGTTAGCCTTTCTCAGACCGCAACCTTAATACCACCTTCTTTGATCCAGCTGGAGGGGGAG980.1636060100166945No HitAGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGG970.16193656093489148TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCACACGTCTGAACTCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGGG880.14691151919866444Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCGGGGGGGGGGGGGGGG860.14357262103505844TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTG850.1419031719532554TruSeq Adapter, Index 22 (97% over 37bp)AGGGCTAGTGTGAGGGCTAGGTTTGGTGCTATAATGAATATTAATATGGAGGATGTGAGGG850.1419031719532554No HitAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGG820.13689482470784642TruSeq Adapter, Index 22 (97% over 37bp)ACGCTTACTAATTGGAAGCCTCTTCGCAGGATACATCATTTCCAACAATATTCCTCCAACA790.1318864774624374No HitAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGTGGGG790.1318864774624374TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGG770.12854757929883137TruSeq Adapter, Index 22 (97% over 37bp)AGACTCAATGGAAGCTATTCTAACGAATGAATCTCATTTGGTCCAATACTGTAGTTATAGA770.12854757929883137No HitAGTAGGGGAAGGGAGCCTACTAGGGTGTAGAATAGGAAGTATGTGCCTGCGTTCAGGCGTT710.11853088480801337No HitCATCATCTGTTAGACTGGTCCAAGACAATTTACTAATTGGCCGCCCTGATATGTAGATCGG710.11853088480801337No HitAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGTGTG700.11686143572621034TruSeq Adapter, Index 22 (97% over 37bp)CCTACGGCCTACTCCAACCCATCGCCGATGCCCTAAAACTATTCACCAAAGAACCCCTACG700.11686143572621034No HitAGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGG630.10517529215358931TruSeq Adapter, Index 22 (97% over 37bp)AGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGG620.1035058430717863TruSeq Adapter, Index 22 (97% over 37bp)Adapter Content