FastQCFastQC Report
Mon 8 Jul 2024
AM-AL-EC2_S86_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-EC2_S86_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59900
Sequences flagged as poor quality0
Sequence length61
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGG2705745.17028380634391TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGT29034.846410684474123TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGTG11771.964941569282137TruSeq Adapter, Index 22 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC10011.671118530884808No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGTT7481.2487479131886476TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTGG7281.2153589315525877TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTGT3730.6227045075125208TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTATCTGGGGGGGGGGGGGGGG3390.5659432387312188TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGTGGG1900.31719532554257096TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTTT1700.2838063439065108TruSeq Adapter, Index 22 (97% over 37bp)
CTTGGCGGTGCTTCACATCCACCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGATAGA1500.25041736227045075No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGG1420.23706176961602674TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATTTGGGGGGGGGGGGGGG1360.2270450751252087TruSeq Adapter, Index 22 (97% over 37bp)
TGAAGAAGTAAACCATTATCTCGGCAATAATGAGCCAAGCTAGTATTTGCGGTGAATCCCC1180.1969949916527546No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGTTG1100.18363939899833057TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGG1090.18196994991652754TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGG1070.17863105175292154TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCT1050.17529215358931552TruSeq Adapter, Index 22 (97% over 37bp)
GGACATCAATGATACTGAAGCTATGAGTATACAGACTATGAAGACCTCACCTTTGACTCAT1040.17362270450751252No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGG1030.17195325542570952TruSeq Adapter, Index 22 (97% over 37bp)
CGGAATTTCCACAATACATGCAAACCCCTCTGATATCATTTGAGAATACAAACTCTTGTTG1020.17028380634390652No Hit
TTACAAAGGACGATGCTACCACATCGAGCCCGTTCCAGGAGAAGAAACTCAATTTATTGCG1010.1686143572621035No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATGTGGGGGGGGGGGGGGG1010.1686143572621035TruSeq Adapter, Index 22 (97% over 37bp)
TGAAGAAGTAAACCATTATCCCGGCAATAATGAGCCAAGCTAGTATTTGCGGTGAATCCCC1000.1669449081803005No Hit
GGTTAGCCTTTCTCAGACCGCAACCTTAATACCACCTTCTTTGATCCAGCTGGAGGGGGAG980.1636060100166945No Hit
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGG970.16193656093489148TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACCGCAACTAATCTGGGGGGGGGGGGGGGG880.14691151919866444Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCGGGGGGGGGGGGGGGG860.14357262103505844TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTG850.1419031719532554TruSeq Adapter, Index 22 (97% over 37bp)
AGGGCTAGTGTGAGGGCTAGGTTTGGTGCTATAATGAATATTAATATGGAGGATGTGAGGG850.1419031719532554No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGGGG820.13689482470784642TruSeq Adapter, Index 22 (97% over 37bp)
ACGCTTACTAATTGGAAGCCTCTTCGCAGGATACATCATTTCCAACAATATTCCTCCAACA790.1318864774624374No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGTGGGG790.1318864774624374TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGG770.12854757929883137TruSeq Adapter, Index 22 (97% over 37bp)
AGACTCAATGGAAGCTATTCTAACGAATGAATCTCATTTGGTCCAATACTGTAGTTATAGA770.12854757929883137No Hit
AGTAGGGGAAGGGAGCCTACTAGGGTGTAGAATAGGAAGTATGTGCCTGCGTTCAGGCGTT710.11853088480801337No Hit
CATCATCTGTTAGACTGGTCCAAGACAATTTACTAATTGGCCGCCCTGATATGTAGATCGG710.11853088480801337No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGGGGGGGTGTG700.11686143572621034TruSeq Adapter, Index 22 (97% over 37bp)
CCTACGGCCTACTCCAACCCATCGCCGATGCCCTAAAACTATTCACCAAAGAACCCCTACG700.11686143572621034No Hit
AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGGGGGG630.10517529215358931TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTGG620.1035058430717863TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)