FastQCFastQC Report
Mon 8 Jul 2024
AM-AL-EC2.merged.rmdup.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-EC2.merged.rmdup.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865
Sequences flagged as poor quality0
Sequence length31-91
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTC60.32171581769436997No Hit
TGAAGAAGTAAACCATTATCTCGGCAATAATGAGCCAAGCTAGTATTTGC40.21447721179624668No Hit
ACGCTTACTAATTGGAAGCCTCTTCGCAGGATACATCATTTCCAACAATA40.21447721179624668No Hit
GCTTATTTAGCTGACCTTACTTTAGGATGGGGTGTGATAGGTGGCACGGA30.16085790884718498No Hit
TTTGAAGTCCTTGAGAGAGGATTATGATGCGACTGTGAGTGCGTTCGTAG30.16085790884718498No Hit
TGAAGAAGTAAACCATTATCCCGGCAATAATGAGCCAAGCTAGTATTTGC30.16085790884718498No Hit
CCCCTAATAAAATTAATTAACCACTCATTCATCGACCTCCCCACCCCATC30.16085790884718498No Hit
TGAAGAAGTAAACCATTATCCAGGCAATAATGAGCCAAGCTAGTATTTGC20.10723860589812334No Hit
CATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCT20.10723860589812334No Hit
CCGTCCTCCTGTTTGGCCGGCTTGGGCAGGAATTCACGCCGACGCTCGAT20.10723860589812334No Hit
GGACATCAATGATACTGAAGCTATGAGTATACAGACTATGAAGACCTCAC20.10723860589812334No Hit
CTCTGCCCTTATCCTTCCATCTACCTGGTGGGTTAGCCTGTGGCTTCCTT20.10723860589812334No Hit
TTTGCATATGCAATCTTATGATCCATCCCTAATAAACTAGGAGGTGTACT20.10723860589812334No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)