##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-EC1_S62_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	96008
Sequences flagged as poor quality	0
Sequence length	61
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.4176526956087	40.0	40.0	40.0	40.0	40.0
2	39.49016748604283	40.0	40.0	40.0	40.0	40.0
3	39.36880259978335	40.0	40.0	40.0	40.0	40.0
4	39.51908174318807	40.0	40.0	40.0	40.0	40.0
5	39.259311724023	40.0	40.0	40.0	40.0	40.0
6	39.39834180484959	40.0	40.0	40.0	40.0	40.0
7	39.33797183568036	40.0	40.0	40.0	40.0	40.0
8	39.043996333638866	40.0	40.0	40.0	40.0	40.0
9	39.338763436380304	40.0	40.0	40.0	40.0	40.0
10	39.44754603783018	40.0	40.0	40.0	40.0	40.0
11	39.40521623198067	40.0	40.0	40.0	40.0	40.0
12	39.47479376718607	40.0	40.0	40.0	40.0	40.0
13	37.90325806182818	40.0	40.0	40.0	24.0	40.0
14	39.408424297975166	40.0	40.0	40.0	40.0	40.0
15	39.47491875677027	40.0	40.0	40.0	40.0	40.0
16	39.20669110907424	40.0	40.0	40.0	40.0	40.0
17	39.400549954170486	40.0	40.0	40.0	40.0	40.0
18	39.36242813098909	40.0	40.0	40.0	40.0	40.0
19	39.352095658695106	40.0	40.0	40.0	40.0	40.0
20	24.506832763936337	24.0	12.0	40.0	12.0	40.0
21	25.754937088575954	24.0	12.0	40.0	12.0	40.0
22	26.90004999583368	24.0	12.0	40.0	12.0	40.0
23	36.50224981251562	40.0	40.0	40.0	24.0	40.0
24	38.42663111407383	40.0	40.0	40.0	40.0	40.0
25	25.455545371219063	24.0	12.0	40.0	12.0	40.0
26	37.55337055245396	40.0	40.0	40.0	24.0	40.0
27	38.06274477126906	40.0	40.0	40.0	40.0	40.0
28	22.006207816015333	12.0	12.0	40.0	12.0	40.0
29	33.316390300808266	40.0	24.0	40.0	24.0	40.0
30	21.326347804349638	12.0	12.0	40.0	12.0	40.0
31	21.886509457545205	12.0	12.0	40.0	12.0	40.0
32	26.86659445046246	24.0	24.0	40.0	12.0	40.0
33	27.947983501374885	24.0	24.0	40.0	12.0	40.0
34	35.98104324639613	40.0	40.0	40.0	24.0	40.0
35	19.87871844012999	12.0	12.0	24.0	12.0	40.0
36	20.78880926589451	12.0	12.0	24.0	12.0	40.0
37	23.662069827514372	24.0	12.0	40.0	12.0	40.0
38	30.270227481043246	24.0	24.0	40.0	12.0	40.0
39	20.07011915673694	12.0	12.0	24.0	12.0	40.0
40	21.502958086826098	24.0	12.0	24.0	12.0	40.0
41	21.508665944504624	12.0	12.0	24.0	12.0	40.0
42	18.698816765269562	12.0	12.0	24.0	12.0	40.0
43	26.297808515957005	24.0	24.0	40.0	12.0	40.0
44	18.139446712773935	12.0	12.0	24.0	12.0	40.0
45	25.57351470710774	24.0	24.0	24.0	12.0	40.0
46	20.74847929339222	24.0	12.0	24.0	12.0	40.0
47	17.195108740938256	12.0	12.0	12.0	12.0	40.0
48	23.034747104407966	24.0	12.0	24.0	12.0	40.0
49	17.36526122823098	12.0	12.0	24.0	12.0	40.0
50	23.374010499125074	24.0	24.0	24.0	12.0	40.0
51	16.60482459795017	12.0	12.0	12.0	12.0	40.0
52	24.07232730605783	24.0	24.0	24.0	12.0	40.0
53	16.34378385134572	12.0	12.0	12.0	12.0	40.0
54	16.745479543371385	12.0	12.0	24.0	12.0	24.0
55	22.38150987417715	24.0	12.0	24.0	12.0	24.0
56	16.19515040413299	12.0	12.0	12.0	12.0	24.0
57	20.087659361719858	24.0	12.0	24.0	12.0	24.0
58	21.92802683109741	24.0	12.0	24.0	12.0	24.0
59	18.35013748854262	12.0	12.0	24.0	12.0	24.0
60	15.770894092158986	12.0	12.0	12.0	12.0	24.0
61	20.74458378468461	24.0	12.0	24.0	12.0	24.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.1437515812900756
1101	2	0.07057525936374986
1101	3	0.17820466007039215
1101	4	-0.01515671791257489
1101	5	-0.2990180075730038
1101	6	0.05276183549836588
1101	7	0.01218441112613533
1101	8	0.47652386564298155
1101	9	0.08724803635734446
1101	10	-0.20688360124525929
1101	11	0.007311361945241401
1101	12	-0.36535133528175834
1101	13	0.4057537169151928
1101	14	-0.03328925988235909
1101	15	-0.03503953593698128
1101	16	0.14989157353866034
1101	17	-0.05446995029167567
1101	18	0.13137139380177842
1101	19	0.0849183158187401
1101	20	-1.4216255363023436
1101	21	-2.267445668580887
1101	22	-0.028987953338674544
1101	23	-0.657504638473128
1101	24	-0.15388613459590061
1101	25	-1.6191257872122229
1101	26	0.2679890011276669
1101	27	-0.015234564283424845
1101	28	-1.0168380559140608
1101	29	-0.33143420798597134
1101	30	-1.1003190388829402
1101	31	-1.1416823423191822
1101	32	0.6436954065441931
1101	33	-0.04377027548820678
1101	34	0.5952537474234489
1101	35	-0.6854941954830593
1101	36	-1.343083466581838
1101	37	0.619106278064244
1101	38	1.2104211400038913
1101	39	-0.5837175667264383
1101	40	-0.9451285267030798
1101	41	0.0926761123512918
1101	42	0.11247072883805131
1101	43	0.35133764826931113
1101	44	0.11276897286812826
1101	45	-0.04367785494688903
1101	46	-0.7999387044794588
1101	47	-0.2905088335173822
1101	48	0.4854130461692172
1101	49	-0.26322797997077174
1101	50	-0.09147910598281328
1101	51	-0.3589073605652118
1101	52	0.9033782585214674
1101	53	-0.8778299036117243
1101	54	0.15060371100597436
1101	55	0.30455200412183814
1101	56	-0.42301276311412117
1101	57	0.4908175067040119
1101	58	-0.16996960922809734
1101	59	-0.2665283118452457
1101	60	-0.4477747956694813
1101	61	-1.2829443341559426
1102	1	-0.06214605231613035
1102	2	-0.05263048226304079
1102	3	0.05530903717476576
1102	4	0.08718246337397062
1102	5	-0.2619809705359657
1102	6	0.3405520180253916
1102	7	0.09223934644423082
1102	8	0.7373780212339796
1102	9	0.17797991656291146
1102	10	-0.00654690090856036
1102	11	0.03287400592893874
1102	12	0.0752387763609903
1102	13	0.19513879577395699
1102	14	0.2300458455580099
1102	15	-0.08811414164316744
1102	16	0.13691088950534436
1102	17	0.13177849911466666
1102	18	0.09052432663892063
1102	19	0.10924048487775195
1102	20	-0.2567309766709407
1102	21	-0.6883210894563092
1102	22	0.9597591669873928
1102	23	0.17361356106612646
1102	24	0.06877911438513706
1102	25	-0.6423403893741941
1102	26	0.027514076442209046
1102	27	0.18669703238501256
1102	28	-0.22523784326121898
1102	29	-0.3816291397598519
1102	30	0.4880647995008971
1102	31	-0.19821255674413152
1102	32	0.07498195625179349
1102	33	0.5645940697182432
1102	34	0.6082344314567649
1102	35	-0.03499800551318799
1102	36	0.4591050856067156
1102	37	1.072499863036775
1102	38	-0.5844574706641943
1102	39	0.534880696608667
1102	40	-0.023012630902975673
1102	41	0.2531404043945322
1102	42	-0.12153600516868224
1102	43	0.09952123855816453
1102	44	0.8394657653543938
1102	45	-0.2522548529975701
1102	46	-0.36107639719610063
1102	47	0.15110023967064024
1102	48	0.07304373906306694
1102	49	0.12078761457113885
1102	50	0.7373353544105932
1102	51	0.8341193982510227
1102	52	-0.06817942356253326
1102	53	0.3322533854189338
1102	54	1.1304459934798352
1102	55	0.7816918233669234
1102	56	0.5446462834922947
1102	57	0.10844111116972144
1102	58	0.6584461270823745
1102	59	-0.1707902292650587
1102	60	0.41107687897166656
1102	61	-0.24205296431720313
1103	1	0.06854083439216652
1103	2	-0.1895171905592079
1103	3	0.22988530836120447
1103	4	-0.1673224429596587
1103	5	-0.10265893663765979
1103	6	0.07650206262842119
1103	7	0.2113740476039112
1103	8	0.120785692955657
1103	9	0.08377831085372378
1103	10	-0.0624791042983901
1103	11	-0.2946460654359129
1103	12	-0.1270359783223327
1103	13	-0.514317047223372
1103	14	-0.21348671465608504
1103	15	-0.5811560684941881
1103	16	-0.030305881235065613
1103	17	-0.26233390052851036
1103	18	-0.18396273848150457
1103	19	0.04095324134519274
1103	20	0.43006652555920866
1103	21	1.0506619613911496
1103	22	1.522025001064108
1103	23	-0.08865227301058809
1103	24	0.14665601001404127
1103	25	0.07487638136621655
1103	26	-0.05830215906180314
1103	27	0.18874877190329897
1103	28	0.45469079188597306
1103	29	0.0010202804007164445
1103	30	-0.10939282761774649
1103	31	1.168424374567195
1103	32	1.4678008679377896
1103	33	0.5248081642766742
1103	34	-0.03810811983672835
1103	35	-0.3656402891884767
1103	36	0.18537627204739238
1103	37	1.1364606123677277
1103	38	0.8420367309415155
1103	39	0.21235616494051257
1103	40	-0.466991221447131
1103	41	0.7950672554204026
1103	42	0.4835487405940313
1103	43	-0.8897740335203395
1103	44	0.1409376654435981
1103	45	-1.090256637118891
1103	46	-0.47588460415417444
1103	47	0.1489592940863389
1103	48	-0.4391774919806437
1103	49	-0.13416332209255444
1103	50	-0.4749750826991317
1103	51	-0.7915273457275678
1103	52	0.27321219106726247
1103	53	-0.6239464361689357
1103	54	-0.27735953729625606
1103	55	-0.12455260125395284
1103	56	-0.8047738770786257
1103	57	-0.589819370543033
1103	58	-0.4564958889747146
1103	59	-0.49616935504561255
1103	60	-0.34423088195607754
1103	61	-1.1109646503118533
1104	1	-0.00907144660994419
1104	2	0.07057525936374986
1104	3	-0.14502766316193316
1104	4	-0.19024089299675495
1104	5	-0.15086985942485143
1104	6	-0.02804624530971722
1104	7	0.01218441112613533
1104	8	0.07248346160258023
1104	9	-0.04743209832278694
1104	10	-0.03179942616108633
1104	11	-0.08696473233084845
1104	12	0.22724125731083689
1104	13	-0.13296682180534702
1104	14	-0.006353232946331389
1104	15	0.045768544871101824
1104	16	-0.3618929382458518
1104	17	-0.014065909887641226
1104	18	-0.01677675434636683
1104	19	-0.17097394007351596
1104	20	0.0867919721151651
1104	21	-0.2203076214428421
1104	22	-1.2411091654598856
1104	23	0.015896034927550318
1104	24	0.1693461886364176
1104	25	0.5626923946059605
1104	26	0.13330886644752837
1104	27	-0.31153086057972246
1104	28	-0.23569327476928237
1104	29	-0.12941400596577068
1104	30	1.135371196807295
1104	31	0.3532671526303126
1104	32	-0.16438540153661663
1104	33	-0.811447043164975
1104	34	-0.5225913704216723
1104	35	1.0653475553586915
1104	36	0.5424384189400477
1104	37	0.45749011644808135
1104	38	0.025235954818704442
1104	39	0.8438918608829873
1104	40	-0.0023675839421386513
1104	41	0.1734841931593749
1104	42	0.16634278271010672
1104	43	-0.18064888371722176
1104	44	0.058896918996072856
1104	45	-0.46792027918931467
1104	46	1.0586471541063993
1104	47	0.6387840957755486
1104	48	-0.4304118696556998
1104	49	-0.020803737546529533
1104	50	-0.010671025174733728
1104	51	0.5030455013876534
1104	52	-0.03938268423947733
1104	53	-0.022611048392867872
1104	54	0.32568788609015087
1104	55	0.3718920714619074
1104	56	0.3311959910946314
1104	57	-0.03443501854851405
1104	58	0.3014108621523732
1104	59	-1.1284811737981073
1104	60	0.1313497834550965
1104	61	-0.29304534425695294
1201	1	-0.03966435137503055
1201	2	-0.3604903637548489
1201	3	-0.13343268467954061
1201	4	-0.021043399295948007
1201	5	-0.09641805662867853
1201	6	-0.16415345426460703
1201	7	0.16813687171435987
1201	8	0.4048579794075593
1201	9	0.20168155476437022
1201	10	0.08285707260137087
1201	11	0.13543587417571246
1201	12	0.1122725860507785
1201	13	0.18241659291581414
1201	14	0.06901412718924149
1201	15	0.0403278241985916
1201	16	0.08000953014668255
1201	17	0.0889064182502537
1201	18	-0.2177712467315871
1201	19	0.21215090662749247
1201	20	0.43392778944965826
1201	21	0.4838643410072656
1201	22	0.36216418615783397
1201	23	0.8010098663989922
1201	24	0.6022402506695528
1201	25	-0.7824588975651992
1201	26	-0.2398264603301854
1201	27	0.5859650700287915
1201	28	0.2902727876206228
1201	29	-1.5671467417117526
1201	30	1.6784621504942763
1201	31	0.9917491023705409
1201	32	0.6642987913859884
1201	33	0.31607542210583617
1201	34	0.3340099630008524
1201	35	0.4406903876551631
1201	36	-0.34056378856546843
1201	37	0.48152739876350736
1201	38	1.0173201522009165
1201	39	0.19408250908688274
1201	40	0.03955272287883105
1201	41	1.7085255560153065
1201	42	0.35859644516244416
1201	43	0.9384895201893926
1201	44	0.6201384736151105
1201	45	1.5982156942314276
1201	46	-0.47871320444601295
1201	47	0.7687370469205526
1201	48	1.2731134497636596
1201	49	0.9778806783564278
1201	50	1.2821244795412987
1201	51	0.4459179343263102
1201	52	1.468767247751515
1201	53	0.8399215624841112
1201	54	1.2354477362473517
1201	55	1.1736402374484847
1201	56	0.7741687651932345
1201	57	1.05031981455069
1201	58	0.26484208386174046
1201	59	-0.16930887687746576
1201	60	0.7695641567688085
1201	61	0.7142907094367175
1202	1	-0.0053778776989830135
1202	2	0.019495903936572745
1202	3	0.14059331811122178
1202	4	0.06808371315393913
1202	5	0.28316953113627363
1202	6	0.28351980538676713
1202	7	0.4176662079122835
1202	8	0.3458976480602445
1202	9	0.10404927054988633
1202	10	-0.028286031343341733
1202	11	0.06442654949520943
1202	12	0.16206327908938079
1202	13	-0.010898873650440066
1202	14	0.09098582091446161
1202	15	0.07567744434521728
1202	16	0.18052116938564922
1202	17	-0.009921905743631498
1202	18	0.33546359789398394
1202	19	0.061405596653848704
1202	20	0.3902501886168608
1202	21	-0.6582207550081485
1202	22	0.7818855531870064
1202	23	0.7344303186792231
1202	24	0.04457562908325485
1202	25	-0.33394398029128425
1202	26	0.06817593174937997
1202	27	0.18484876623468693
1202	28	0.8758007062899189
1202	29	0.04958895564588772
1202	30	1.2129811874607626
1202	31	0.8600518333280363
1202	32	0.24533048644014244
1202	33	-0.08300318428633346
1202	34	0.6582696365262919
1202	35	0.4437469337580673
1202	36	0.5468977277471829
1202	37	2.2357633729822055
1202	38	0.11568334961392424
1202	39	0.3699889521974704
1202	40	-0.37339108540042076
1202	41	0.28262465012591775
1202	42	1.1911730249316506
1202	43	-0.056261194112142476
1202	44	0.713739660795337
1202	45	0.012311244520471831
1202	46	-0.34373790045257024
1202	47	0.29262461918128935
1202	48	0.03308598514650285
1202	49	0.26495237429653784
1202	50	-0.04756167076103068
1202	51	0.22846018332407425
1202	52	-0.13374886556217902
1202	53	0.41014460175408374
1202	54	1.1882263138459699
1202	55	-0.19516084341709572
1202	56	0.71618298477728
1202	57	1.1155261314126363
1202	58	-0.3421259552974867
1202	59	0.2409770652253478
1202	60	0.6005681657169575
1202	61	-1.7870714469787075
1203	1	-0.12802383401870543
1203	2	-0.11612394746968135
1203	3	0.08551037945664319
1203	4	-0.1881619378976609
1203	5	-0.24184674234804504
1203	6	-0.10663978699050602
1203	7	0.054531822601063595
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2403	30	-0.614686009560593
2403	31	0.007544554480531929
2403	32	0.8162951907944596
2403	33	-0.2844946715524088
2403	34	0.7928281974250808
2403	35	0.18783456134084275
2403	36	-0.04008585290461042
2403	37	0.14105717865592027
2403	38	0.03255836990810934
2403	39	0.5132063651489496
2403	40	0.859716035423034
2403	41	-0.33202396283422075
2403	42	-0.42088875597774233
2403	43	-0.07336242691620143
2403	44	-0.46282515612405817
2403	45	0.22960513678270544
2403	46	0.3729552839485102
2403	47	-0.23179875538982841
2403	48	0.3058831190664755
2403	49	0.2919679927009291
2403	50	0.05993797747310481
2403	51	-0.4568446623860076
2403	52	-0.651981160915625
2403	53	-0.4939261410671847
2403	54	-0.5047524394958067
2403	55	-0.8361756608776716
2403	56	0.4951657861323895
2403	57	-0.4259227110070789
2403	58	0.03440422621369876
2403	59	0.4404770165193064
2403	60	-0.1606315182349416
2403	61	1.2400199363811453
2404	1	-0.1385549201448626
2404	2	-0.17028998471692347
2404	3	0.05370132013296569
2404	4	-0.09804346317874035
2404	5	0.04348526740615455
2404	6	-0.18204063724623154
2404	7	0.15582878628176644
2404	8	0.3948496746440284
2404	9	0.007392676759216954
2404	10	-0.13677198129441592
2404	11	0.10873286529600534
2404	12	-0.14709156718276262
2404	13	0.16120750452331833
2404	14	-0.21300712448768166
2404	15	-0.11307606413428317
2404	16	0.1888232266182257
2404	17	-0.14051799775451457
2404	18	0.2444420792197306
2404	19	-0.13762869772505582
2404	20	0.09688215583878801
2404	21	1.1927078494504428
2404	22	-0.21818972453401386
2404	23	-0.5889520168658393
2404	24	-0.3359424967165765
2404	25	0.4319530612942799
2404	26	-0.009209565459968871
2404	27	-0.13493099266153052
2404	28	-0.060912238251908946
2404	29	0.4321718417937106
2404	30	-0.0014850397273988847
2404	31	-0.03033305090558258
2404	32	-0.1337251007798521
2404	33	0.1037715955263323
2404	34	-0.4854785487429325
2404	35	0.48332150709470056
2404	36	-0.10922288866981233
2404	37	-0.37233005742353953
2404	38	-1.771206497572301
2404	39	0.14974784841099975
2404	40	0.4331559255363544
2404	41	-0.6611428922715703
2404	42	-0.8723398636895823
2404	43	-0.7454609919376907
2404	44	-0.8202482302455394
2404	45	-0.14276086078230676
2404	46	-0.019140367411026205
2404	47	-0.1988743753234381
2404	48	-0.2564536378196536
2404	49	-0.2713261102553979
2404	50	0.1269105786448108
2404	51	-0.21305440442286638
2404	52	-0.1599885283179887
2404	53	-0.6085995507800863
2404	54	-0.32610333804834113
2404	55	-0.3446408894440438
2404	56	0.3111888456855567
2404	57	-0.9383942247585217
2404	58	-0.3510732501388105
2404	59	1.1553647885044462
2404	60	0.07371015025276328
2404	61	0.9577693406413346
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
13	1.0
14	5.0
15	17.0
16	85.0
17	128.0
18	175.0
19	249.0
20	400.0
21	562.0
22	944.0
23	1708.0
24	3916.0
25	13220.0
26	25248.0
27	15125.0
28	5217.0
29	3034.0
30	3327.0
31	4006.0
32	3693.0
33	2315.0
34	1874.0
35	1669.0
36	1895.0
37	1406.0
38	1103.0
39	2809.0
40	1877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	5.512098076181945	75.0486943660358	9.483683481412813	9.955524076369432
2	70.61911507374386	4.507957670194151	11.652153987167736	13.22077326889426
3	5.704732938921756	71.46071160736605	9.328389300891594	13.506166152820597
4	5.522456461961503	5.484959586701108	77.29772518956753	11.694858761769853
5	3.6736521956503627	5.377676860261644	9.645029580868261	81.30364136321974
6	72.74706274477127	4.981876510290809	10.914715440379968	11.356345304557953
7	71.84088825931173	6.299475043746354	7.8660111657361895	13.993625531205733
8	6.1505291225731185	72.85747021081576	12.13961336555287	8.852387301058245
9	6.075535372052329	74.14590450795767	7.774352137321889	12.004207982668111
10	72.16065327889342	9.750229147571035	7.644154653778852	10.444962919756687
11	6.551537371885677	75.41454878760103	7.6462378135155395	10.387676026997749
12	74.25631197400216	7.647279393383885	6.417173568869261	11.679235063744688
13	7.0869094242146495	10.171027414382134	7.792058995083743	74.95000416631947
14	74.61253228897591	9.808557620198318	7.294183818015165	8.2847262728106
15	7.3681359886676105	8.512832263978002	73.36055328722605	10.758478460128321
16	7.881634863761352	10.697025247896008	7.457711857345221	73.96362803099741
17	75.45517040246646	10.676193650529124	6.2411465711190734	7.627489375885343
18	8.651362386467794	9.313807182734772	73.14077993500541	8.894050495792017
19	76.6550704107991	10.355387051079076	6.303641363219732	6.685901174902091
20	20.111865677860177	11.622989750854096	61.02303974668778	7.24210482459795
21	12.37188567619365	73.22098158486793	5.724522956420299	8.682609782518123
22	77.01754020498292	11.411549037580201	5.426631114073827	6.144279643363053
23	78.62782268144322	10.243938005166237	5.1735272060661615	5.95471210732439
24	78.29243396383634	11.438630114157155	4.9579201733188905	5.311015748687609
25	18.80780768269311	70.69515040413299	5.10790767436047	5.3891342388134325
26	13.567619365052913	76.58945087909342	5.02562286476127	4.817306891092409
27	12.544787934338805	76.3186401133239	5.6641113240563286	5.4724606282809765
28	27.653945504541287	61.6417381884843	4.781893175568703	5.922423131405717
29	16.202816431964003	73.8198900091659	5.25789517540205	4.719398383468045
30	56.01408215982001	32.50041663194734	5.699525039580035	5.7859761686526126
31	21.86692775602033	7.964961253228897	65.2435213732189	4.924589617531872
32	81.14948754270478	7.362928089325889	7.27335222064828	4.214232147321057
33	13.55526159550866	10.0409345151916	71.41562594394159	4.98817794535815
34	14.385259561703192	10.49183401383218	71.78985084576284	3.333055578701775
35	32.567077743521374	9.829389217565202	53.540329972502285	4.063203066411132
36	15.509124239646695	11.114698775102076	12.046912757270228	61.32926422798101
37	17.987042746437798	8.019123406382802	67.84851262394801	6.145321223231397
38	14.214440463294725	11.182401466544455	71.22739771685693	3.3757603533038916
39	26.431130739105075	9.273185567869344	61.049079243396385	3.246604449629197
40	14.345679526706107	12.055245396216982	9.579410049162568	64.01966502791434
41	15.223731355720357	10.328305974502126	66.17052745604532	8.277435213732188
42	27.319598366802765	11.637571869010916	58.354512123989664	2.6883176401966504
43	14.764702206112117	9.620232068829033	72.56838114284524	3.0466845822136115
44	29.55587034413799	11.45217065244563	56.37030247479377	2.621656528622615
45	12.475132021623423	9.749289114335413	74.64247398627182	3.133104877769329
46	14.702941421548205	11.049079243396385	9.732522289809182	64.51545704524622
47	51.90088325972836	33.43888009332556	10.747021081576536	3.913215565369552
48	10.502249812515624	70.85972002333139	14.161319890009166	4.476710274143821
49	10.369969169235898	5.995333722189818	38.97696025331223	44.65773685526206
50	11.768810932422298	4.948545954503791	13.763436380301641	69.51920673277226
51	10.89596700274977	4.995417048579285	44.93792183984668	39.170694108824264
52	8.426381134905425	7.090034163819682	14.683151404049664	69.80043329722523
53	21.290114262501692	9.89615340548085	19.492328684366765	49.32140364765069
54	67.55166236146988	10.89805016248646	17.2089825847846	4.341304891259061
55	7.6639446712773935	7.418131822348138	79.58086826097825	5.337055245396217
56	36.73964669610866	5.330805766186151	53.361178235147065	4.5683693025581205
57	70.3430964086326	5.357886842763103	16.815265394550455	7.483751354053829
58	8.252523253512765	4.940264772360349	81.02430031143562	5.782911662691262
59	5.77347721023248	5.065202899758353	21.736730272477296	67.42458961753186
60	26.735272060661615	8.35034580451629	54.43713023914674	10.47725189567536
61	8.320226650140095	5.260033122584812	14.512483464747362	71.90725676252772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	2.0
2	2.5
3	1.0
4	1.0
5	1.0
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	0.5
18	0.0
19	0.0
20	2.0
21	4.0
22	4.5
23	5.0
24	3.5
25	5.0
26	8.0
27	7.0
28	6.0
29	5.5
30	13.0
31	21.0
32	17.0
33	13.0
34	21.0
35	39.0
36	49.0
37	89.5
38	130.0
39	291.0
40	977.0
41	1502.0
42	3045.5
43	7491.5
44	10394.0
45	13173.5
46	15953.0
47	16707.5
48	17179.5
49	16897.0
50	14008.5
51	11120.0
52	9286.5
53	5968.0
54	4483.0
55	3391.0
56	2299.0
57	1697.0
58	869.0
59	643.0
60	511.5
61	313.5
62	247.0
63	209.5
64	172.0
65	157.0
66	122.5
67	103.0
68	86.5
69	70.0
70	71.5
71	54.5
72	36.0
73	33.0
74	30.0
75	24.0
76	17.0
77	16.0
78	15.0
79	14.0
80	11.5
81	6.0
82	3.0
83	2.0
84	3.0
85	5.0
86	3.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	2.0
94	2.0
95	0.0
96	2.5
97	5.0
98	3.5
99	28.5
100	55.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010415798683443048
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0010415798683443048
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0020831597366886096
44	0.0
45	0.0010415798683443048
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0010415798683443048
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0010415798683443048
59	0.0
60	0.0
61	0.0010415798683443048
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	96008.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.73356386967753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.62667178154805	53.53720523289726
2	5.023627264642801	6.0015832013998835
3	1.703604247676507	3.0528705941171568
4	0.8666236551639959	2.070660778268478
5	0.5266002894557882	1.5727856011999002
6	0.36617900922422364	1.3123906341138238
7	0.28596836910844126	1.1957336888592618
8	0.20575772899265898	0.9832513957170237
9	0.16913982807023664	0.9092992250645779
>10	1.0566879980470452	12.37188567619365
>50	0.09067289752218871	3.7121906507791023
>100	0.06800467314164153	7.677485209565869
>500	0.008718547838671991	3.713232230647446
>1k	0.0017437095677343983	1.8894258811765687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	1814	1.8894258811765687	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCCCCGTTGTCTC	883	0.919715023748021	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCCCCGTTGTCTC	814	0.8478460128322639	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCCCCGTTGTCTC	737	0.7676443629697525	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCTCCGTTGTCTC	566	0.5895342054828765	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCCGTTGTCTC	565	0.5884926256145322	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGACCCCCGTTGTCTC	475	0.4947504374635447	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCCCCGTTGTCTC	373	0.38850929089242564	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCCCCGTTGTCGC	351	0.36559453378885093	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCCCCGTTGTCGC	341	0.3551787351054079	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCTCCGTTGTCTC	338	0.35205399550037497	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGACCTCCGTTGTCTC	283	0.29476710274143825	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCCCCGTGGTCTC	252	0.2624781268227648	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCCCCGTTGTCGC	249	0.2593533872177318	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCCGTGGTCTC	248	0.2583118073493876	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCCCCGTGGTCTC	242	0.2520623281393217	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCTCCGTGGTCTC	226	0.23539705024581284	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGATCTCCGTTGTCTC	220	0.229147571035747	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCTCCGTTGTCTC	217	0.2260228314307141	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAATCCCCGTTGTCTC	202	0.21039913340554955	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCCCCGTGGTCTC	197	0.205191234063828	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAATCTCCGTTGTCTC	185	0.19269227564369637	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGATCCCCGTTGTCTC	167	0.17394383801349886	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGACCCCCGTTGTCGC	165	0.17186067827681026	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGACCCCCGTGGTCTC	161	0.16769435880343303	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTTTCTTTTTCGATCTCCGTTGTCTC	147	0.1531122406466128	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCCGTTGTCGC	145	0.15102908090992417	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCCCCGTGGTCTC	142	0.14790434130489125	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCGGTTGTCTC	139	0.14477960169985835	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCTCCGTTGTCGC	137	0.14269644196316975	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAATCCCCGTTGTCTC	135	0.14061328222648112	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCGGTGGTCTC	133	0.13853012248979252	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCCCCATTGTCTC	129	0.1343638030164153	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCCCCGTGGTCGC	128	0.13332222314807102	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCCCCGTGGTCGC	125	0.13019748354303806	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCCCCGTGGTCGC	121	0.12603116406966086	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTGCTTTCTTTTTCGACCCCCGTTGTCTC	119	0.12394800433297225	Illumina Single End PCR Primer 1 (96% over 33bp)
GTTCAATAAGGGCTCCAGAAGATGTTGATCGTAAGTGAGAAGATAGATCGGAAGAGCGTCG	118	0.12290642446462796	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCTGTTGTCTC	118	0.12290642446462796	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCCCCATTGTCTC	115	0.11978168485959505	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTTTCTTTTTCGACCTCCGTTGTCTC	114	0.11874010499125073	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCTCCGTTGTCGC	109	0.11353220564952919	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTTTCTTTTTCGACCCCCGTTGTCTC	103	0.10728272643946338	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTTTCTTTTTCAACCCCCGTTGTCTC	101	0.10519956670277478	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCTCCGTGGTCTC	101	0.10519956670277478	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTGCTTTCTTTTTCAACCCCCGTTGTCTC	97	0.10103324722939755	Illumina Single End PCR Primer 1 (96% over 33bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	65.5632863928006	0.0	0.0	0.0	0.0
2	65.78722606449463	0.0	0.0	0.0	0.0
3	65.94346304474627	0.0	0.0	0.0	0.0
4	66.07678526789434	0.0	0.0	0.0	0.0
5	66.22781434880427	0.0	0.0	0.0	0.0
6	66.51008249312557	0.0	0.0	0.0	0.0
7	66.8704691275727	0.0	0.0	0.0	0.0
8	67.784976251979	0.0	0.0	0.0	0.0
9	68.42450629114241	0.0	0.0	0.0	0.0
10	69.16402799766686	0.0	0.0	0.0	0.0
11	69.89105074577118	0.0	0.0	0.0	0.0
12	70.8295142071494	0.0	0.0	0.0	0.0
13	72.04607949337556	0.0	0.0	0.0	0.0
14	73.40429964169653	0.0	0.0	0.0	0.0
15	75.10728272643946	0.0	0.0	0.0	0.0
16	77.00087492708941	0.0	0.0	0.0	0.0
17	78.24868760936589	0.0	0.0	0.0	0.0
18	80.07874343804683	0.0	0.0	0.0	0.0
19	81.29010082493126	0.0	0.0	0.0	0.0
20	82.49104241313223	0.0	0.0	0.0	0.0
21	83.6680276643613	0.0	0.0	0.0	0.0
22	84.17840179985001	0.0	0.0	0.0	0.0
23	85.05957836846929	0.0	0.0	0.0	0.0
24	86.13969669194233	0.0	0.0	0.0	0.0
25	86.62611449045913	0.0	0.0	0.0	0.0
26	87.24689609199234	0.0	0.0	0.0	0.0
27	87.74060494958754	0.0	0.0	0.0	0.0
28	88.26972752270645	0.0	0.0	0.0	0.0
29	88.95404549620865	0.0010415798683443046	0.0	0.0	0.0
30	89.59357553537205	0.0010415798683443046	0.0	0.0	0.0
31	90.19040079993334	0.0010415798683443046	0.0	0.0	0.0
32	90.78722606449463	0.0010415798683443046	0.0	0.0	0.0
33	91.27051912340639	0.0010415798683443046	0.0	0.0	0.0
34	91.7152737271894	0.002083159736688609	0.0	0.0	0.0
35	92.01212398966753	0.002083159736688609	0.0	0.0	0.0
36	92.23398050162487	0.002083159736688609	0.0	0.0	0.0
37	92.3923006416132	0.002083159736688609	0.0	0.0	0.0
38	92.47875177068578	0.002083159736688609	0.0	0.0	0.0
39	92.52458128489293	0.002083159736688609	0.0	0.0	0.0
40	92.57457711857346	0.002083159736688609	0.0	0.0	0.0
41	92.68915090409132	0.002083159736688609	0.0	0.0	0.0
42	92.75477043579701	0.002083159736688609	0.0	0.0	0.0
43	92.82768102658112	0.002083159736688609	0.0	0.0	0.0
44	93.01620698275144	0.002083159736688609	0.0	0.0	0.0
45	95.26081159903342	0.002083159736688609	0.0	0.0	0.0
46	95.35976168652613	0.002083159736688609	0.0	0.0	0.0
47	95.40975752020665	0.002083159736688609	0.0	0.0	0.0
48	95.43892175652029	0.002083159736688609	0.0	0.0	0.0
49	95.48787601033247	0.002083159736688609	0.0	0.0	0.0
50	95.5545371219065	0.002083159736688609	0.0	0.0	0.0
>>END_MODULE