AM-AL-EC1_S62_R2_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	AM-AL-EC1_S62_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	96008
Sequences flagged as poor quality	0
Sequence length	61
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	1814	1.8894258811765687	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCCCCGTTGTCTC	883	0.919715023748021	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCCCCGTTGTCTC	814	0.8478460128322639	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCCCCGTTGTCTC	737	0.7676443629697525	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCTCCGTTGTCTC	566	0.5895342054828765	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCCGTTGTCTC	565	0.5884926256145322	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGACCCCCGTTGTCTC	475	0.4947504374635447	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCCCCGTTGTCTC	373	0.38850929089242564	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCCCCGTTGTCGC	351	0.36559453378885093	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCCCCGTTGTCGC	341	0.3551787351054079	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCTCCGTTGTCTC	338	0.35205399550037497	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGACCTCCGTTGTCTC	283	0.29476710274143825	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCCCCGTGGTCTC	252	0.2624781268227648	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCCCCGTTGTCGC	249	0.2593533872177318	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCCGTGGTCTC	248	0.2583118073493876	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCCCCGTGGTCTC	242	0.2520623281393217	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCTCCGTGGTCTC	226	0.23539705024581284	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGATCTCCGTTGTCTC	220	0.229147571035747	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCTCCGTTGTCTC	217	0.2260228314307141	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAATCCCCGTTGTCTC	202	0.21039913340554955	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCCCCGTGGTCTC	197	0.205191234063828	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAATCTCCGTTGTCTC	185	0.19269227564369637	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGATCCCCGTTGTCTC	167	0.17394383801349886	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGACCCCCGTTGTCGC	165	0.17186067827681026	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCGACCCCCGTGGTCTC	161	0.16769435880343303	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTTTCTTTTTCGATCTCCGTTGTCTC	147	0.1531122406466128	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCCGTTGTCGC	145	0.15102908090992417	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCCCCGTGGTCTC	142	0.14790434130489125	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCGGTTGTCTC	139	0.14477960169985835	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCTCCGTTGTCGC	137	0.14269644196316975	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAATCCCCGTTGTCTC	135	0.14061328222648112	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCGGTGGTCTC	133	0.13853012248979252	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCCCCATTGTCTC	129	0.1343638030164153	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCTTTCTTTTTCAACCCCCGTGGTCGC	128	0.13332222314807102	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGACCCCCGTGGTCGC	125	0.13019748354303806	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCCCCGTGGTCGC	121	0.12603116406966086	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTGCTTTCTTTTTCGACCCCCGTTGTCTC	119	0.12394800433297225	Illumina Single End PCR Primer 1 (96% over 33bp)
GTTCAATAAGGGCTCCAGAAGATGTTGATCGTAAGTGAGAAGATAGATCGGAAGAGCGTCG	118	0.12290642446462796	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCGATCTCTGTTGTCTC	118	0.12290642446462796	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCCCCATTGTCTC	115	0.11978168485959505	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTTTCTTTTTCGACCTCCGTTGTCTC	114	0.11874010499125073	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCTCCGTTGTCGC	109	0.11353220564952919	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTTTCTTTTTCGACCCCCGTTGTCTC	103	0.10728272643946338	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTTTCTTTTTCAACCCCCGTTGTCTC	101	0.10519956670277478	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTTCTTTCTTTTTCAACCTCCGTGGTCTC	101	0.10519956670277478	Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTTGCTTTCTTTTTCAACCCCCGTTGTCTC	97	0.10103324722939755	Illumina Single End PCR Primer 1 (96% over 33bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content