##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-EC1_S62_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96008 Sequences flagged as poor quality 0 Sequence length 61 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.13846762769769 40.0 40.0 40.0 40.0 40.0 2 38.9333805516207 40.0 40.0 40.0 40.0 40.0 3 38.65540371635697 40.0 40.0 40.0 40.0 40.0 4 39.014707107741025 40.0 40.0 40.0 40.0 40.0 5 38.7627697691859 40.0 40.0 40.0 40.0 40.0 6 38.83355553703858 40.0 40.0 40.0 40.0 40.0 7 38.987042746437794 40.0 40.0 40.0 40.0 40.0 8 38.7468544287976 40.0 40.0 40.0 40.0 40.0 9 39.09111740688276 40.0 40.0 40.0 40.0 40.0 10 39.128197650195816 40.0 40.0 40.0 40.0 40.0 11 39.2403133072244 40.0 40.0 40.0 40.0 40.0 12 39.25922839763353 40.0 40.0 40.0 40.0 40.0 13 37.96550287476044 40.0 40.0 40.0 24.0 40.0 14 39.2404382968086 40.0 40.0 40.0 40.0 40.0 15 39.45754520456629 40.0 40.0 40.0 40.0 40.0 16 39.31697358553454 40.0 40.0 40.0 40.0 40.0 17 39.29530872427298 40.0 40.0 40.0 40.0 40.0 18 39.295725356220316 40.0 40.0 40.0 40.0 40.0 19 39.35334555453712 40.0 40.0 40.0 40.0 40.0 20 39.32126489459212 40.0 40.0 40.0 40.0 40.0 21 39.478085159570036 40.0 40.0 40.0 40.0 40.0 22 39.24027164402966 40.0 40.0 40.0 40.0 40.0 23 39.264186317806846 40.0 40.0 40.0 40.0 40.0 24 39.33668027664361 40.0 40.0 40.0 40.0 40.0 25 39.57432713940505 40.0 40.0 40.0 40.0 40.0 26 39.4462128155987 40.0 40.0 40.0 40.0 40.0 27 39.3828014332139 40.0 40.0 40.0 40.0 40.0 28 39.32497291892342 40.0 40.0 40.0 40.0 40.0 29 39.27210232480627 40.0 40.0 40.0 40.0 40.0 30 39.25535372052329 40.0 40.0 40.0 40.0 40.0 31 39.39613365552871 40.0 40.0 40.0 40.0 40.0 32 39.47308557620198 40.0 40.0 40.0 40.0 40.0 33 39.28693442213149 40.0 40.0 40.0 40.0 40.0 34 39.266977751854014 40.0 40.0 40.0 40.0 40.0 35 39.28351804016332 40.0 40.0 40.0 40.0 40.0 36 39.2658528455962 40.0 40.0 40.0 40.0 40.0 37 39.31984834597117 40.0 40.0 40.0 40.0 40.0 38 39.29072577285226 40.0 40.0 40.0 40.0 40.0 39 39.292808932588954 40.0 40.0 40.0 40.0 40.0 40 39.31809849179235 40.0 40.0 40.0 40.0 40.0 41 38.57849345887843 40.0 40.0 40.0 40.0 40.0 42 35.56103658028498 40.0 40.0 40.0 24.0 40.0 43 38.82563953003916 40.0 40.0 40.0 40.0 40.0 44 38.78547621031581 40.0 40.0 40.0 40.0 40.0 45 28.578660111657364 24.0 24.0 40.0 12.0 40.0 46 32.34767936005333 40.0 24.0 40.0 24.0 40.0 47 33.62344804599617 40.0 24.0 40.0 24.0 40.0 48 38.18002666444463 40.0 40.0 40.0 40.0 40.0 49 34.650445796183654 40.0 24.0 40.0 24.0 40.0 50 34.74243813015582 40.0 24.0 40.0 24.0 40.0 51 30.179568369302558 24.0 24.0 40.0 12.0 40.0 52 33.042829764186315 40.0 24.0 40.0 24.0 40.0 53 27.459753353887177 24.0 24.0 40.0 12.0 40.0 54 23.470794100491627 24.0 12.0 40.0 12.0 40.0 55 26.634572118990086 24.0 24.0 40.0 12.0 40.0 56 26.411382384801268 24.0 24.0 40.0 12.0 40.0 57 23.82693108907591 24.0 12.0 40.0 12.0 40.0 58 21.835680359970002 12.0 12.0 40.0 12.0 40.0 59 20.42613115573702 12.0 12.0 24.0 12.0 40.0 60 20.113157236896924 12.0 12.0 24.0 12.0 40.0 61 19.991959003416383 12.0 12.0 24.0 12.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2274652496136511 1101 2 0.21947608796416063 1101 3 0.41367817533212303 1101 4 0.05606776714527939 1101 5 -0.15295628735403 1101 6 0.017427382497423594 1101 7 0.18183809834403064 1101 8 0.23267279456761258 1101 9 -0.05028674015370882 1101 10 0.1648001655039053 1101 11 0.19832225031128559 1101 12 0.09782355189517489 1101 13 -0.16155792793086476 1101 14 0.13605243161892844 1101 15 -0.06931828410014163 1101 16 0.23194050207787598 1101 17 0.2260963822057107 1101 18 -0.09046603948321774 1101 19 -0.04705648347405145 1101 20 0.24134710689642702 1101 21 0.29638492193598154 1101 22 -0.032682559688240076 1101 23 0.3403448333608097 1101 24 0.07205860134817499 1101 25 0.024589453461608457 1101 26 0.021036527143998285 1101 27 0.014614254404882843 1101 28 -0.0780474409747427 1101 29 0.4349731557588328 1101 30 0.4209398182429922 1101 31 0.08041050674127348 1101 32 -0.07606232062917684 1101 33 -0.13552127205809938 1101 34 -0.059730400015197915 1101 35 0.1270301041345192 1101 36 0.22607773942745268 1101 37 0.026788505472957524 1101 38 -0.06370098323342432 1101 39 -0.12776514585542742 1101 40 0.11941261058583308 1101 41 -0.06489641260182566 1101 42 0.4132882412458656 1101 43 -0.15019994546667448 1101 44 0.4375854024509991 1101 45 -0.4174762391311049 1101 46 0.2235033070783814 1101 47 -0.7932502085708037 1101 48 0.1859928529980337 1101 49 -0.9225234079582805 1101 50 -0.8769544961748394 1101 51 -1.2931879274193285 1101 52 -0.8090084419306649 1101 53 -0.3412160283059187 1101 54 -1.727419020006682 1101 55 -1.194681464674538 1101 56 -0.5597597456516681 1101 57 -0.9948071596129218 1101 58 -0.8321726917086032 1101 59 -0.9814104274103634 1101 60 -1.406248243317016 1101 61 -0.9254257266131773 1102 1 0.4657250405050206 1102 2 0.19922976508625823 1102 3 0.5666553151513654 1102 4 -0.05535346269700625 1102 5 -0.21466991485712583 1102 6 0.3583364734065171 1102 7 0.0682460373835525 1102 8 0.3073228034281499 1102 9 0.3664685318647187 1102 10 0.17937574586895266 1102 11 0.24958044630632514 1102 12 0.15156269773958542 1102 13 0.15152908252456143 1102 14 0.2142466545499957 1102 15 0.1019115581823371 1102 16 0.09681734063892122 1102 17 0.03865176055055741 1102 18 -0.033625706327093496 1102 19 0.24507536128937346 1102 20 -0.23588131770041798 1102 21 0.3671362953189359 1102 22 0.19459016758448655 1102 23 0.006922894675710722 1102 24 -0.033647583305373985 1102 25 -0.05945272268582613 1102 26 0.27059350038518204 1102 27 0.18460362176266898 1102 28 -0.06223138568500275 1102 29 -0.006192890670369877 1102 30 -0.007378452180539341 1102 31 0.1298079903492848 1102 32 0.27875781050674675 1102 33 0.3295239166712989 1102 34 0.24617957694741222 1102 35 0.38974497211254544 1102 36 0.20937563062008024 1102 37 0.040743848375669245 1102 38 0.24189887499801443 1102 39 -0.03021065356409025 1102 40 -0.06648141741345626 1102 41 0.20512839689666862 1102 42 0.8759853881535449 1102 43 0.42972208182376903 1102 44 0.16454801099255434 1102 45 0.8159545600891676 1102 46 1.1694540425028066 1102 47 -0.04962979389775768 1102 48 0.4848144018195839 1102 49 -0.042406448893956394 1102 50 -0.9748634098732225 1102 51 0.1437693648011198 1102 52 -0.1710942119111749 1102 53 -1.0456728774996122 1102 54 -0.5312024685269741 1102 55 0.5766281224245233 1102 56 -0.7561092051590244 1102 57 -0.1639547761289606 1102 58 -0.6702907140371508 1102 59 -0.41637409921613866 1102 60 0.43838227236613037 1102 61 -0.4159449539745097 1103 1 0.331648323288249 1103 2 0.010425126370826376 1103 3 0.570089748692844 1103 4 -0.044782543874525516 1103 5 0.21775649013841303 1103 6 0.01003138866075659 1103 7 0.366372709997286 1103 8 0.3346644626609745 1103 9 0.18729814827863578 1103 10 0.05203408663612663 1103 11 0.24908981294325372 1103 12 0.2834538663390518 1103 13 -0.3525297934790004 1103 14 0.22731534322082325 1103 15 0.04878934587189576 1103 16 0.24266925856934307 1103 17 -0.07513057664836964 1103 18 -0.0549013530710738 1103 19 0.31438847458107233 1103 20 -0.14114447559515497 1103 21 -0.07117771003343165 1103 22 0.07086135402516192 1103 23 0.12083904097365661 1103 24 -0.16219352442936952 1103 25 -0.032021857386986596 1103 26 -0.07762237829456353 1103 27 -0.15165864407140361 1103 28 -0.05558553376707209 1103 29 -0.0821072528469955 1103 30 0.06666258260982261 1103 31 0.03681066653125953 1103 32 0.12358385867801758 1103 33 -0.19496314844912632 1103 34 0.1794980426030719 1103 35 0.12328645293324314 1103 36 -0.21065113119972523 1103 37 0.07691690814373686 1103 38 -0.014211740523840888 1103 39 -0.32307416828309243 1103 40 0.08891554958029957 1103 41 0.020248825085779742 1103 42 -0.034808545833968196 1103 43 -0.5378961162136946 1103 44 0.012407065408254425 1103 45 0.6682739178054931 1103 46 0.7430490469631081 1103 47 1.029451383622309 1103 48 0.3110855882602621 1103 49 0.7437666108741112 1103 50 0.39739350359688785 1103 51 -0.64896034081886 1103 52 1.7014749227899841 1103 53 1.8361736880668467 1103 54 1.4709830800903347 1103 55 0.5365745987849166 1103 56 1.3363082792299146 1103 57 1.0443413880102028 1103 58 1.0455879657130716 1103 59 0.19459824690339644 1103 60 -0.12660434672396548 1103 61 0.03062061248400738 1104 1 0.18706120920960956 1104 2 0.27334814183621603 1104 3 0.588762350416296 1104 4 0.1368758479533554 1104 5 -0.4492525836503276 1104 6 0.1790435441135898 1104 7 -0.23567031916438452 1104 8 -0.023219461324643476 1104 9 0.31334962348265094 1104 10 0.28601228671602286 1104 11 0.03670608869512648 1104 12 -0.009920555848928814 1104 13 -0.3635781299510725 1104 14 -0.03903174346524452 1104 15 -0.001978216760072371 1104 16 0.24540851554588983 1104 17 -0.016327860218531498 1104 18 -0.30595425497143225 1104 19 -0.1952046316222038 1104 20 -0.08188521633589829 1104 21 8.862563968392578E-5 1104 22 0.03465750765182918 1104 23 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-0.3197818496444782 2304 17 -1.0198502637409348 2304 18 -0.22856497758215255 2304 19 -0.06788010429767155 2304 20 -0.5411444755951607 2304 21 -0.026588401037344056 2304 22 -0.38233290933859365 2304 23 -0.3748584818425087 2304 24 -0.23729130800172982 2304 25 -0.11754988867772909 2304 26 0.034763540871011855 2304 27 0.016789856580487594 2304 28 0.1318981689708636 2304 29 -0.11105119026551336 2304 30 0.09221668951986572 2304 31 -0.07322844689768004 2304 32 0.13766469309783957 2304 33 -0.12169065822748593 2304 34 -0.2228487631335625 2304 35 0.03306481016922902 2304 36 -0.0025676892179760102 2304 37 0.3866952653797213 2304 38 -0.10182582135826124 2304 39 -0.1504535685438455 2304 40 0.3254214165946365 2304 41 -0.023818971524384835 2304 42 -0.5232048952472681 2304 43 0.034985239718508865 2304 44 0.043697808563401 2304 45 -0.9963936180484829 2304 46 -0.6710317874567089 2304 47 -0.7186581339787352 2304 48 0.33481440181958533 2304 49 -0.8541473395822123 2304 50 -0.5355921548124982 2304 51 -0.6244492586806594 2304 52 -0.04376627668850119 2304 53 -0.3523530394429315 2304 54 -0.1224980150856787 2304 55 0.5065876365945634 2304 56 -0.15287033876226275 2304 57 -0.7692179340236969 2304 58 0.10582264628673599 2304 59 0.21884857284863912 2304 60 -0.19987683694560943 2304 61 -0.46533766652511943 2401 1 0.14993556682080822 2401 2 0.19922976508625823 2401 3 0.224550051993468 2401 4 0.19464653730299375 2401 5 0.48269850619550425 2401 6 0.687283841827572 2401 7 0.22614077422565515 2401 8 0.228375435007095 2401 9 -0.06774199445106888 2401 10 0.24516521955316506 2401 11 0.31536991999053754 2401 12 0.5068258556343253 2401 13 0.6515290825245614 2401 14 0.2668782334973656 2401 15 0.07559576870865214 2401 16 0.33365944590207874 2401 17 0.13075702370845477 2401 18 0.32163745156763923 2401 19 0.24507536128937346 2401 20 0.19832920861536962 2401 21 -0.05391633626001635 2401 22 0.20774806232132903 2401 23 0.2042913157283408 2401 24 0.3610892587998862 2401 25 -0.019979038475298694 2401 26 0.2311198161746546 2401 27 0.32934046386793625 2401 28 0.0956633511570999 2401 29 0.1911755303822602 2401 30 0.25577944255630314 2401 31 0.24822904298086002 2401 32 0.370863073664637 2401 33 0.11899760088182632 2401 34 -0.030136212526272743 2401 35 0.4292186563230729 2401 36 0.20937563062008024 2401 37 0.2644280589019843 2401 38 0.2682146644716923 2401 39 0.4303156622253823 2401 40 0.4072027931128588 2401 41 0.6524968179492987 2401 42 0.9549327565745926 2401 43 0.6139326081395637 2401 44 0.46717958993992426 2401 45 -0.18404543991083244 2401 46 -0.22528279960246067 2401 47 1.4635281008390848 2401 48 -0.06781717712778601 2401 49 0.2602251300534135 2401 50 0.8014523796004553 2401 51 0.6700851542748048 2401 52 0.2631163144046198 2401 53 0.4411692277635453 2401 54 0.3898501630519746 2401 55 0.45820706979294457 2401 56 0.08599605799886945 2401 57 -0.4007968813921181 2401 58 -0.18344860877399327 2401 59 -0.24532146763719354 2401 60 -0.5353019381601847 2401 61 -0.8238396908166159 2402 1 -0.012502286770825322 2402 2 0.16849943763897812 2402 3 0.15730335218205482 2402 4 0.0324658412632175 2402 5 0.20273965140380312 2402 6 0.03993256461173189 2402 7 0.2593998175995651 2402 8 0.22473236968907884 2402 9 0.21538847014690532 2402 10 0.0030085248848976676 2402 11 0.41043249499482215 2402 12 0.18315021702640166 2402 13 0.7559779081717437 2402 14 -0.04183940751069315 2402 15 0.10589750023616773 2402 16 -0.0020168784593082023 2402 17 0.39648649396389857 2402 18 0.025306232640843973 2402 19 0.37922588417639247 2402 20 0.25494672961657017 2402 21 0.35552134589325135 2402 22 0.09524163338253544 2402 23 0.11810067626323217 2402 24 0.15519177894389458 2402 25 0.270523275942665 2402 26 0.36475602395795903 2402 27 0.18807524871278503 2402 28 0.3482349266757012 2402 29 0.6000988974318062 2402 30 0.27292327934697624 2402 31 0.5113869377177025 2402 32 0.4240089677157286 2402 33 -0.03233961838784438 2402 34 0.3934879945712737 2402 35 0.3665150732611835 2402 36 0.23967736214759583 2402 37 0.17682305290164635 2402 38 0.37604939788503344 2402 39 0.2894361833980241 2402 40 0.370172105645004 2402 41 0.3338357516026491 2402 42 0.8593558664665863 2402 43 0.1359624384155751 2402 44 0.6823347416628778 2402 45 1.0239521599520174 2402 46 0.8282916903683955 2402 47 -0.23456893327715278 2402 48 0.510058701493854 2402 49 1.1042590748502619 2402 50 0.6485722150196267 2402 51 1.1099531467315167 2402 52 0.9793858155189668 2402 53 0.5719767024763875 2402 54 0.32586079223078457 2402 55 0.2593814226131066 2402 56 -1.044125663242344 2402 57 -0.36856646822565153 2402 58 -0.6031725930016627 2402 59 0.31301043704307574 2402 60 -0.5315731536582113 2402 61 -0.47646269784558726 2403 1 -0.7835510623735402 2403 2 -0.3565103132649554 2403 3 -0.33987679181507957 2403 4 -0.2594498687607114 2403 5 -0.024029478815570826 2403 6 -0.2508868275643579 2403 7 -0.4868553082134497 2403 8 -0.1314865987179985 2403 9 -0.1426014732451435 2403 10 -0.12129705263215129 2403 11 -0.06659226703041554 2403 12 -0.25352161498396697 2403 13 0.40161424689179626 2403 14 -0.12781602642428425 2403 15 0.21207166719272408 2403 16 0.2352094373856417 2403 17 0.00982362224362987 2403 18 -0.04823139530682852 2403 19 0.08317789918751117 2403 20 0.10222122019771973 2403 21 -0.14832103733308344 2403 22 -0.3151185702795942 2403 23 0.16081490625806083 2403 24 0.17117271987977034 2403 25 0.08996815961701543 2403 26 0.042480742762975865 2403 27 0.22918716800692351 2403 28 -0.03842794504456748 2403 29 -0.38304521565759586 2403 30 -0.1516468902660435 2403 31 -0.03230238267065033 2403 32 0.10072170081504339 2403 33 0.010796272317698197 2403 34 -0.0837897638803895 2403 35 0.0060794977470237654 2403 36 -0.06838795309649726 2403 37 -0.10501496613633776 2403 38 -0.08828337674786013 2403 39 0.38258103439366664 2403 40 0.26821454579553716 2403 41 -0.09954712686419498 2403 42 0.313389578240411 2403 43 0.1676713238609011 2403 44 -0.23372315235270236 2403 45 0.5994241564100662 2403 46 -0.707227953749964 2403 47 0.1964526452097175 2403 48 0.14258139211084853 2403 49 0.01814541620569088 2403 50 0.030501945262187746 2403 51 0.7123692626038789 2403 52 0.590488142031937 2403 53 -0.49001796351562277 2403 54 0.19759160403306808 2403 55 -0.3025236404778795 2403 56 -0.8446801470769252 2403 57 -1.2190561217259628 2403 58 -0.5462062309848612 2403 59 -0.1708452284956472 2403 60 -0.011328168785290416 2403 61 -0.17527045218605863 2404 1 -0.6186377958463112 2404 2 -0.4889154371476181 2404 3 -0.31619288251321365 2404 4 -0.39327019987757694 2404 5 -0.10640286459481985 2404 6 -0.1778371600339952 2404 7 0.11410828141807627 2404 8 -0.12299535531276007 2404 9 0.005620672666886151 2404 10 0.25760387245551897 2404 11 0.14419036338197344 2404 12 0.22860619071640542 2404 13 1.042162015338917 2404 14 0.09599483529123631 2404 15 0.05964552332025619 2404 16 0.14784120254449107 2404 17 0.39983808649403585 2404 18 0.4708166697094569 2404 19 0.13820448635283356 2404 20 0.09293912569101792 2404 21 0.061712365720012485 2404 22 0.15439724153947054 2404 23 0.1643945480857596 2404 24 0.08405422749171265 2404 25 -0.049679495405406726 2404 26 0.17451125296599201 2404 27 0.012535589946800485 2404 28 0.24869262853059837 2404 29 0.17700223540849436 2404 30 0.09805393908701632 2404 31 0.022640957010310103 2404 32 -0.012619749148015558 2404 33 -0.1652721571144511 2404 34 -0.0018742392651489581 2404 35 -0.2468263597540954 2404 36 0.16770191256287603 2404 37 0.04120924015092697 2404 38 0.01927237305182672 2404 39 -0.11711199050773757 2404 40 -0.11538986215876434 2404 41 -0.06433344097776228 2404 42 0.6876286442036346 2404 43 0.3533132139950368 2404 44 0.05814252783127216 2404 45 0.7076875098268545 2404 46 0.9795741981975041 2404 47 0.0945993480890408 2404 48 -0.34878688435404825 2404 49 0.013525180823428684 2404 50 0.4235965664340995 2404 51 -0.8247955361973567 2404 52 -1.2853690469086345 2404 53 -0.779511090395193 2404 54 -0.5206676910688586 2404 55 -1.8604170628860217 2404 56 -1.4066422902834113 2404 57 -0.991082885792185 2404 58 -1.428879307707824 2404 59 -0.18545516200171974 2404 60 -0.8611184891840473 2404 61 0.6107939189312255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 3.0 17 13.0 18 15.0 19 24.0 20 46.0 21 55.0 22 85.0 23 125.0 24 184.0 25 256.0 26 404.0 27 499.0 28 738.0 29 907.0 30 1169.0 31 1532.0 32 2373.0 33 4349.0 34 14080.0 35 28893.0 36 12419.0 37 2829.0 38 5277.0 39 12290.0 40 7443.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.258772797816825 74.83620985969773 10.1294697261658 6.775547616319644 2 77.04031669965622 6.5100531305344305 8.178976976768414 8.27065319304094 3 8.661778185151238 75.21144071327389 9.326306141154904 6.800474960419965 4 7.762894758770103 6.872343971335722 76.60403299725023 8.760728272643945 5 8.539913340554953 6.365094575452046 9.49920839930006 75.59578368469295 6 77.20398300141656 6.92025664527956 7.60249145904508 8.273268894258813 7 77.62686442796434 7.532705607866011 9.042996416965252 5.7974335472044 8 6.033872177318557 85.90429964169653 2.7331055745354553 5.328722606449462 9 16.462169819181735 74.872927256062 6.0953253895508706 2.5695775352053993 10 74.17819348387634 16.484042996416964 5.9807516040329975 3.3570119156736937 11 5.987001083243063 72.85538705107908 17.344387967669363 3.813223898008499 12 74.6614865427881 5.18290142488126 6.0567869344221315 14.098825097908508 13 17.68290142488126 6.0432463961336556 2.2435630364136325 74.03028914257145 14 16.987126072827262 74.77710190817432 2.2591867344387966 5.9765852845596195 15 7.196275310390801 17.77768519290059 4.730855762019832 70.29518373468878 16 7.383759686692776 84.04299641696525 2.7310224147987667 5.842221481543205 17 15.27789350887426 8.583659695025414 3.3080576618615116 72.83038913423881 18 75.28539288392633 14.414423798016832 5.844304641279893 4.455878676776935 19 14.219648362636446 7.384801266561119 70.66598616781936 7.729564202983084 20 9.693983834680443 7.729564202983084 3.9746687776018663 78.60178318473461 21 12.576035330389136 8.345137905174568 73.8105157903508 5.268310974085493 22 75.94367136071995 8.626364469627532 6.632780601616532 8.797183568035997 23 9.789809182568119 76.89775852012333 4.236105324556287 9.07632697275227 24 13.599908340971586 75.57703524706274 4.517331889009249 6.30572452295642 25 11.22718940088326 9.99291725689526 6.900466627781018 71.87942671444047 26 9.526289475877011 11.349054245479543 73.30847429380884 5.816181984834597 27 12.641654862094825 9.791892342304807 3.6757353553870513 73.89071744021332 28 8.913840513290559 11.500083326389468 5.863053078910091 73.72302308140988 29 11.039705024581286 75.94992083993 5.715148737605199 7.2952253978835095 30 77.34980418298476 10.743896341971503 3.786142821431547 8.120156653612199 31 7.5806182818098495 12.336471960669943 72.22731439046746 7.855595367052745 32 8.070160819931672 9.853345554537121 6.826514457128573 75.24997916840263 33 10.052287309390884 75.38225981168236 5.43704691275727 9.128405966169485 34 6.873385551204065 9.95958670110824 7.926422798100158 75.24060494958754 35 8.271185734522122 74.2948504291309 7.7056078660111655 9.728355970335805 36 9.713773852178985 75.77493542204816 6.144279643363053 8.3670110824098 37 74.60524122989752 11.666736105324556 4.910007499375052 8.818015165402883 38 75.07707691025747 10.478293475543705 6.123448045996167 8.32118156820265 39 7.666027831014083 77.51124906257812 5.387051079076743 9.435672027331057 40 74.27193567202734 10.422048162653113 5.280809932505624 10.025206232813932 41 6.711940671610699 77.60394967086076 5.666194483793017 10.017915173735522 42 7.844137988500958 11.619865011249063 4.753770519123406 75.78222648112657 43 5.458920089992501 77.40709107574368 6.642154820431631 10.49183401383218 44 6.18698441796517 9.825222898091827 72.90017498541789 11.087617698525124 45 7.550412465627865 11.080326639446712 6.75568702608116 74.61357386884427 46 9.469002583118073 10.703274727106074 70.07436880259978 9.753353887176068 47 72.58665944504624 12.97391884009666 4.279851679026748 10.159570035830349 48 73.00120823264727 10.743896341971503 6.977543538038497 9.277351887342721 49 73.63032247312724 9.974168819265062 4.981876510290809 11.41363219731689 50 76.32593117240229 11.293850512457295 3.9955003749687523 8.384717940171653 51 75.7686859428381 10.819931672360637 5.199566702774769 8.211815682026497 52 75.37913507207733 13.282226481126571 3.8330139155070415 7.50562453128906 53 74.4969169235897 13.277018581784853 5.321431547371053 6.904632947254395 54 76.10615782018165 11.303224731272394 5.311015748687609 7.279601699858346 55 75.77701858178484 13.159320056661944 4.092367302724774 6.971294058828431 56 76.77172735605366 10.713690525789518 5.268310974085493 7.246271144071327 57 74.83959670027498 8.934672110657445 5.94012998916757 10.28560119990001 58 71.33363886342805 12.203149737521873 9.723148070994084 6.740063328055995 59 62.2437713523873 8.018081826514457 20.328514290475795 9.40963253062245 60 65.23102241479877 9.531497375218732 18.51304057995167 6.7244396300308305 61 58.45137905174569 6.834847096075328 26.96025331222398 7.753520539955004 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.5 10 2.0 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.5 21 3.0 22 2.0 23 1.0 24 3.0 25 6.0 26 7.0 27 6.5 28 6.0 29 7.0 30 9.0 31 10.0 32 11.5 33 13.0 34 13.5 35 23.0 36 32.0 37 21.5 38 11.0 39 17.5 40 28.5 41 33.0 42 65.0 43 179.5 44 262.0 45 389.5 46 517.0 47 1945.0 48 3742.5 49 4112.0 50 5276.0 51 6440.0 52 6656.0 53 5947.0 54 5022.0 55 4618.5 56 4215.0 57 5370.0 58 8380.5 59 10236.0 60 12957.5 61 23169.0 62 30659.0 63 15974.5 64 1290.0 65 711.5 66 112.0 67 91.0 68 94.0 69 97.0 70 89.0 71 61.5 72 42.0 73 35.0 74 28.0 75 20.0 76 16.0 77 20.0 78 14.0 79 8.0 80 8.0 81 11.0 82 14.0 83 7.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0010415798683443048 2 0.018748437630197484 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 61 96008.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.311515707024416 #Duplication Level Percentage of deduplicated Percentage of total 1 75.18958056638533 18.279726689442548 2 15.642003341759136 7.605616198650113 3 4.528512060323036 3.3028497625197897 4 1.6580266483869586 1.6123656361969836 5 0.6169401482370078 0.7499375052078993 6 0.5055481770275481 0.7374385467877677 7 0.26134270168373247 0.4447546037830181 8 0.21849963583394028 0.4249645862844763 9 0.1199605843794182 0.2624781268227648 >10 0.9982434343001586 4.721481543204733 >50 0.1199605843794182 2.098783434713774 >100 0.09425474486954287 4.3350554120489955 >500 0.012852919754937662 2.2581451545704527 >1k 0.025705839509875324 16.64757103574702 >5k 0.004284306584979221 6.641113240563286 >10k+ 0.004284306584979221 29.87771852345638 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGGGG 28685 29.87771852345638 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGGGT 6376 6.641113240563286 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGTGG 3226 3.3601366552787266 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGTGT 3092 3.2205649529205895 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGTTT 2695 2.807057745187901 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGGTT 2621 2.7299808349304224 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGGTG 2533 2.6383218065161236 TruSeq Adapter, Index 7 (97% over 36bp) TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC 1816 1.8915090409132571 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGTTG 815 0.8488875927006083 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTTTT 751 0.7822264811265727 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTGGG 602 0.6270310807432714 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGAATCTGGGGGGGGGGGGGGGG 453 0.47183568035997 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTGTT 405 0.42183984667944335 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTGGT 347 0.36142821431547373 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTTGT 347 0.36142821431547373 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTGTG 250 0.2603949670860761 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTTGG 237 0.2468544287976002 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGG 210 0.21873177235230398 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGG 183 0.19060911590700777 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCT 169 0.17602699775018749 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGG 162 0.16873593867177736 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGTGGGG 141 0.14686276143654697 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGG 125 0.13019748354303806 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGG 123 0.12811432380634946 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATC 122 0.12707274393800516 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTTTG 119 0.12394800433297225 TruSeq Adapter, Index 7 (97% over 36bp) ATCTTCTCACTTACGATCAACATCTTCTGGAGCCCTTATTGAACAGATCGGAAGAGCACAC 118 0.12290642446462796 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATGTGGGGGGGGGGGGGGG 115 0.11978168485959505 TruSeq Adapter, Index 7 (97% over 36bp) CGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGGGG 114 0.11874010499125073 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGG 113 0.11769852512290642 TruSeq Adapter, Index 7 (97% over 36bp) TTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGG 104 0.10832430630780768 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACAT 104 0.10832430630780768 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTG 101 0.10519956670277478 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGAGGGGGGG 100 0.10415798683443046 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTAGGGGGGGGGGGGGG 99 0.10311640696608615 TruSeq Adapter, Index 7 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCGGGGGGGGGGGGGGGG 97 0.10103324722939755 TruSeq Adapter, Index 7 (97% over 36bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 66.44654612115657 0.0 0.0 0.0 0.0 2 66.72256478626781 0.0 0.0 0.0 0.0 3 66.96837763519707 0.0 0.0 0.0 0.0 4 67.19752520623281 0.0 0.0 0.0 0.0 5 67.5027081076577 0.0 0.0 0.0 0.0 6 68.0859928339305 0.0 0.0 0.0 0.0 7 68.91404882926423 0.0 0.0 0.0 0.0 8 76.01137405216232 0.0 0.0 0.0 0.0 9 76.96337805182901 0.0 0.0 0.0 0.0 10 77.70394133822181 0.0 0.0 0.0 0.0 11 78.19140071660695 0.0 0.0 0.0 0.0 12 79.11111574035498 0.0 0.0 0.0 0.0 13 80.1412382301475 0.0 0.0 0.0 0.0 14 81.30676610282477 0.0 0.0 0.0 0.0 15 82.81393217231897 0.0 0.0 0.0 0.0 16 84.4085909507541 0.0 0.0 0.0 0.0 17 85.36580284976252 0.0 0.0 0.0 0.0 18 85.8803433047246 0.0 0.0 0.0 0.0 19 86.88442629780852 0.0 0.0 0.0 0.0 20 87.27814348804266 0.0 0.0 0.0 0.0 21 87.62603116406966 0.0 0.0 0.0 0.0 22 87.97704357970169 0.0 0.0 0.0 0.0 23 88.2780601616532 0.0 0.0 0.0 0.0 24 88.57699358386802 0.0 0.0 0.0 0.0 25 88.8634280476627 0.0 0.0 0.0 0.0 26 89.1196566952754 0.0 0.0 0.0 0.0 27 89.35922006499459 0.0 0.0 0.0 0.0 28 89.6112823931339 0.0 0.0 0.0 0.0 29 89.88000999916673 0.0 0.0 0.0 0.0 30 90.18102658111825 0.0 0.0 0.0 0.0 31 90.51120739938338 0.0 0.0 0.0 0.0 32 90.83513873843846 0.0 0.0 0.0 0.0 33 91.13511374052162 0.0 0.0 0.0 0.0 34 91.36634447129406 0.0 0.0 0.0 0.0 35 91.53091409049246 0.0 0.0 0.0 0.0 36 91.66423631364053 0.0 0.0 0.0 0.0 37 91.78714273810516 0.0 0.0 0.0 0.0 38 91.87359386717773 0.0 0.0 0.0 0.0 39 91.92150654112157 0.0 0.0 0.0 0.0 40 91.97046079493376 0.0 0.0 0.0 0.0 41 92.08399300058329 0.0 0.0 0.0 0.0 42 92.15586201149904 0.0 0.0 0.0 0.0 43 92.2277310224148 0.0 0.0 0.0 0.0 44 92.41729855845347 0.0 0.0 0.0 0.0 45 94.65773685526206 0.0 0.0 0.0 0.0 46 94.75460378301808 0.0 0.0 0.0 0.0 47 94.80355803683027 0.0 0.0 0.0 0.0 48 94.83376385301224 0.0 0.0 0.0 0.0 49 94.88167652695608 0.0 0.0 0.0 0.0 50 94.94833763853012 0.0 0.0 0.0 0.0 >>END_MODULE