FastQCFastQC Report
Mon 8 Jul 2024
AM-AL-EC1_S62_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-EC1_S62_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96008
Sequences flagged as poor quality0
Sequence length61
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGGGG2868529.87771852345638TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGGGT63766.641113240563286TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGTGG32263.3601366552787266TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGTGT30923.2205649529205895TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGTTT26952.807057745187901TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGGTT26212.7299808349304224TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGGTG25332.6383218065161236TruSeq Adapter, Index 7 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC18161.8915090409132571No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGTTG8150.8488875927006083TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTTTT7510.7822264811265727TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTGGG6020.6270310807432714TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGAATCTGGGGGGGGGGGGGGGG4530.47183568035997TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTGTT4050.42183984667944335TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTGGT3470.36142821431547373TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTTGT3470.36142821431547373TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTGTG2500.2603949670860761TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTTGG2370.2468544287976002TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGG2100.21873177235230398TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGG1830.19060911590700777TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCT1690.17602699775018749TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGG1620.16873593867177736TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGTGGGG1410.14686276143654697TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGG1250.13019748354303806TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGG1230.12811432380634946TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATC1220.12707274393800516TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGTTTG1190.12394800433297225TruSeq Adapter, Index 7 (97% over 36bp)
ATCTTCTCACTTACGATCAACATCTTCTGGAGCCCTTATTGAACAGATCGGAAGAGCACAC1180.12290642446462796No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATGTGGGGGGGGGGGGGGG1150.11978168485959505TruSeq Adapter, Index 7 (97% over 36bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGGGGGGGGG1140.11874010499125073TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGG1130.11769852512290642TruSeq Adapter, Index 7 (97% over 36bp)
TTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGG1040.10832430630780768TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACAT1040.10832430630780768TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTG1010.10519956670277478TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTGGGGGGGAGGGGGGG1000.10415798683443046TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCTAGGGGGGGGGGGGGG990.10311640696608615TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGAGACATCGGGGGGGGGGGGGGGG970.10103324722939755TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)