##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-EC06_S56_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88553
Sequences flagged as poor quality	0
Sequence length	61
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.51229207367339	40.0	40.0	40.0	40.0	40.0
2	39.484647612164466	40.0	40.0	40.0	40.0	40.0
3	39.432678734769006	40.0	40.0	40.0	40.0	40.0
4	39.467437579754495	40.0	40.0	40.0	40.0	40.0
5	37.66946348514449	40.0	40.0	40.0	24.0	40.0
6	39.409077049902315	40.0	40.0	40.0	40.0	40.0
7	37.79350219642474	40.0	40.0	40.0	24.0	40.0
8	39.27198400957619	40.0	40.0	40.0	40.0	40.0
9	38.38961977572753	40.0	40.0	40.0	40.0	40.0
10	39.23828667577609	40.0	40.0	40.0	40.0	40.0
11	39.25450295303378	40.0	40.0	40.0	40.0	40.0
12	39.25730353573566	40.0	40.0	40.0	40.0	40.0
13	39.04373651937258	40.0	40.0	40.0	40.0	40.0
14	39.26435016317911	40.0	40.0	40.0	40.0	40.0
15	39.320949036170425	40.0	40.0	40.0	40.0	40.0
16	39.21886327961786	40.0	40.0	40.0	40.0	40.0
17	39.27948234390704	40.0	40.0	40.0	40.0	40.0
18	39.17188576332818	40.0	40.0	40.0	40.0	40.0
19	39.27722380947004	40.0	40.0	40.0	40.0	40.0
20	34.15721658215983	40.0	24.0	40.0	24.0	40.0
21	34.99174505663275	40.0	24.0	40.0	24.0	40.0
22	36.58726412430973	40.0	40.0	40.0	24.0	40.0
23	38.47955461700902	40.0	40.0	40.0	40.0	40.0
24	38.776461554097544	40.0	40.0	40.0	40.0	40.0
25	34.76313620091922	40.0	24.0	40.0	24.0	40.0
26	38.451503619301434	40.0	40.0	40.0	40.0	40.0
27	38.64596343432746	40.0	40.0	40.0	40.0	40.0
28	33.11612254807855	40.0	24.0	40.0	12.0	40.0
29	37.667792169661105	40.0	40.0	40.0	24.0	40.0
30	29.690603367474846	40.0	12.0	40.0	12.0	40.0
31	34.92471175454248	40.0	24.0	40.0	24.0	40.0
32	32.85264192065768	40.0	24.0	40.0	12.0	40.0
33	37.17197610470566	40.0	40.0	40.0	24.0	40.0
34	26.772983411064562	24.0	12.0	40.0	12.0	40.0
35	27.388795410658023	24.0	12.0	40.0	12.0	40.0
36	27.211229433220783	24.0	12.0	40.0	12.0	40.0
37	32.316059309114316	40.0	24.0	40.0	12.0	40.0
38	29.557914469300872	40.0	24.0	40.0	12.0	40.0
39	26.653597280724536	24.0	12.0	40.0	12.0	40.0
40	25.862387496753357	24.0	12.0	40.0	12.0	40.0
41	25.82977425948302	24.0	12.0	40.0	12.0	40.0
42	28.407936490011632	24.0	24.0	40.0	12.0	40.0
43	30.516820435219586	40.0	24.0	40.0	12.0	40.0
44	24.66644834167109	24.0	12.0	40.0	12.0	40.0
45	32.25516922069269	40.0	24.0	40.0	12.0	40.0
46	29.559427687373663	24.0	24.0	40.0	12.0	40.0
47	23.85816403735616	24.0	12.0	40.0	12.0	40.0
48	28.48445563673732	24.0	24.0	40.0	12.0	40.0
49	24.621503506374715	24.0	12.0	40.0	12.0	40.0
50	30.037920793197294	40.0	24.0	40.0	12.0	40.0
51	23.572640113830136	24.0	12.0	40.0	12.0	40.0
52	28.292999672512508	24.0	24.0	40.0	12.0	40.0
53	26.009259991191715	24.0	12.0	40.0	12.0	40.0
54	21.543956726480186	12.0	12.0	40.0	12.0	40.0
55	25.43821214413967	24.0	12.0	40.0	12.0	40.0
56	22.99802378236762	24.0	12.0	40.0	12.0	40.0
57	21.944169028717265	12.0	12.0	40.0	12.0	40.0
58	25.696181947534246	24.0	24.0	40.0	12.0	40.0
59	24.881167210597045	24.0	12.0	40.0	12.0	40.0
60	23.0659604982327	24.0	12.0	24.0	12.0	40.0
61	25.00890991835398	24.0	24.0	24.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.27819311654329937
1101	2	-0.12996564588939208
1101	3	-0.19699701299674643
1101	4	0.2008941617921991
1101	5	0.5019769969341468
1101	6	0.07660348355789637
1101	7	0.348544692400111
1101	8	0.36995144795084656
1101	9	0.860901264888092
1101	10	0.41690989422825453
1101	11	0.3056391250261967
1101	12	0.01681941087774419
1101	13	0.17545587041328758
1101	14	0.179932832254174
1101	15	0.006507533599261706
1101	16	0.15496473554209445
1101	17	0.466803921469527
1101	18	0.29983457695644944
1101	19	0.19948993267661308
1101	20	0.7246156448696581
1101	21	0.5455310835589273
1101	22	0.6189699649351823
1101	23	0.23213865716957827
1101	24	0.06026068833013909
1101	25	0.686663747371604
1101	26	0.5499897606682396
1101	27	0.1715398465377831
1101	28	0.6623884910478779
1101	29	0.1758146307639592
1101	30	1.2662417463023665
1101	31	1.2435561991820308
1101	32	1.3694280251985163
1101	33	0.8816319559643446
1101	34	2.293989437253174
1101	35	1.8559923725721639
1101	36	1.511900208801265
1101	37	0.9083062302014184
1101	38	1.824159677516814
1101	39	1.82452058040613
1101	40	2.2999407757400334
1101	41	2.2991894378177236
1101	42	1.923637491757379
1101	43	1.323052559702539
1101	44	2.379241611080136
1101	45	1.3866965611640296
1101	46	0.5313471571858983
1101	47	1.5912414307575986
1101	48	2.1820457714813912
1101	49	1.7406441272797473
1101	50	0.6908671911942186
1101	51	1.1969863239898508
1101	52	0.829674776315656
1101	53	0.8751885028506834
1101	54	0.9148577127562554
1101	55	0.7402979257485534
1101	56	1.0179326105818376
1101	57	1.2410446127475687
1101	58	0.841568714392114
1101	59	1.4588184757433176
1101	60	0.978821984846487
1101	61	1.0592109769666145
1102	1	0.16143528389920903
1102	2	-0.02520336031220438
1102	3	0.45316892726475544
1102	4	0.14688693772620098
1102	5	0.040862997973768245
1102	6	0.18664386901912877
1102	7	0.530985680311133
1102	8	0.17359546943644943
1102	9	0.2569053700768933
1102	10	0.47603527575757454
1102	11	0.029845077549957466
1102	12	0.08186266197401437
1102	13	-0.11387996868463546
1102	14	-0.01871565429066635
1102	15	0.39298173621965304
1102	16	-0.09614486500171182
1102	17	0.4922347663653639
1102	18	0.06973074766832354
1102	19	0.15294668824461155
1102	20	-0.491586522350147
1102	21	-1.2017332346198657
1102	22	-0.4364367553121937
1102	23	0.2236617088709636
1102	24	0.271331369551568
1102	25	-1.1160472766597493
1102	26	0.5415661265098706
1102	27	0.4859866456900832
1102	28	0.3645223895110945
1102	29	0.22486363978737955
1102	30	-0.6092430791604677
1102	31	0.14672439196596088
1102	32	-0.7497024211741241
1102	33	0.48268224452712616
1102	34	0.021101010420167654
1102	35	-1.4178157231800306
1102	36	0.7717933139500772
1102	37	0.4260265175646367
1102	38	0.225215297493623
1102	39	0.02202281293575581
1102	40	0.3639977152418119
1102	41	0.01334567490570393
1102	42	-0.6636977342279309
1102	43	-0.2637895104855268
1102	44	0.1651586809349901
1102	45	0.002448223898781521
1102	46	0.04101700937244601
1102	47	0.19872940175470788
1102	48	0.4676229636921967
1102	49	0.7821091998536218
1102	50	-0.7793235401424994
1102	51	0.6861302321829008
1102	52	0.2222667765289117
1102	53	0.6127763045753944
1102	54	0.9669989419137579
1102	55	0.3788280549020584
1102	56	0.49198861708616093
1102	57	0.9112433412053242
1102	58	0.7521609012048636
1102	59	0.8951280709557103
1102	60	1.3032385282242025
1102	61	0.29015450396356357
1103	1	0.1689653267069886
1103	2	0.09491629516254818
1103	3	0.08878706125825886
1103	4	0.13966634873244033
1103	5	-0.1900926985551905
1103	6	0.09272463646458817
1103	7	-0.4575129529345219
1103	8	-0.15504448105094326
1103	9	0.0732445314570569
1103	10	0.31171530051388174
1103	11	0.01643541227583256
1103	12	-0.1476489167561894
1103	13	-0.3068759973606916
1103	14	0.2134778574943681
1103	15	-0.0169414160831991
1103	16	-0.025950424855238907
1103	17	-0.33746248465325124
1103	18	-0.1178498392624121
1103	19	-0.23366427673553147
1103	20	-0.6386802352801553
1103	21	-0.11462198597372009
1103	22	0.5658841482929446
1103	23	0.07027576838925143
1103	24	0.09592263263888867
1103	25	0.3608210507423877
1103	26	0.031018909015415375
1103	27	-0.10919618993836622
1103	28	-0.1174519258223441
1103	29	0.3901119764731291
1103	30	0.030449530050940865
1103	31	0.0691546359187214
1103	32	0.34891019422636305
1103	33	0.17848914534717863
1103	34	-0.26194607813519966
1103	35	-0.05070034848303706
1103	36	0.17449587725917226
1103	37	-0.43219205997933585
1103	38	-1.0639692917064387
1103	39	0.026767771417368635
1103	40	0.14052048588495936
1103	41	-0.870815447380128
1103	42	0.6919162737147175
1103	43	-0.22278688012810832
1103	44	-0.5160523149905352
1103	45	0.04798951019512998
1103	46	-0.9293270001174712
1103	47	-0.5116734039598754
1103	48	0.8912136594475193
1103	49	-0.07616095332601702
1103	50	-0.46806457887580066
1103	51	0.04896482911930633
1103	52	0.20127549281134094
1103	53	-0.35246171698322115
1103	54	0.09805263215049109
1103	55	-0.06771379872628813
1103	56	-0.15549191311137278
1103	57	0.4703232318649775
1103	58	0.8565499878003564
1103	59	0.7555128251978047
1103	60	0.2667229781700513
1103	61	1.0082420793929288
1104	1	-0.08731660426997223
1104	2	-0.27595222741069136
1104	3	-0.08043408356500947
1104	4	-0.10201856420677302
1104	5	0.07307068378241866
1104	6	-0.10527348677594972
1104	7	-0.2362638652481479
1104	8	0.11020418957777167
1104	9	-0.11724655393550165
1104	10	0.1997353192814728
1104	11	-0.17958168708815236
1104	12	0.03147734744983666
1104	13	-0.19718983324850115
1104	14	0.11672048078703767
1104	15	-0.22818111997226254
1104	16	-0.7680002632453977
1104	17	0.029652518996002186
1104	18	0.00403526135275456
1104	19	0.24782878968089506
1104	20	-0.9792078132178048
1104	21	-1.2994827929802923
1104	22	0.6728594376266983
1104	23	0.2531555515192636
1104	24	-0.1554588708539697
1104	25	-0.5096825463799135
1104	26	0.04175814371460973
1104	27	-0.38653953265549745
1104	28	-1.4333731019249392
1104	29	0.1736590518562977
1104	30	-0.9735125177021615
1104	31	-0.2102739950895156
1104	32	-0.14707562581325817
1104	33	0.039608945159507414
1104	34	-0.23671408981281417
1104	35	-1.5128002337921806
1104	36	-0.45307163394925354
1104	37	-0.6751281458932112
1104	38	-0.14560832830325054
1104	39	-0.17946724057303953
1104	40	-0.5058146199434184
1104	41	0.33270868983184343
1104	42	-0.552583778417489
1104	43	-0.6134656414668633
1104	44	-0.718325279227841
1104	45	-0.8740207077174951
1104	46	-0.5168569761366584
1104	47	-0.9668918379083671
1104	48	-1.7519935139441998
1104	49	-0.6701553230476307
1104	50	-1.155062806380755
1104	51	-1.3423395187067797
1104	52	-1.2549855852827072
1104	53	-0.3506892219357809
1104	54	0.05801509696125251
1104	55	-0.3792558694175625
1104	56	-0.6217623750027279
1104	57	-0.050686047667920064
1104	58	-0.5583181177152312
1104	59	-1.0844412984597902
1104	60	-0.2897362023116514
1104	61	-0.7036753431907421
1201	1	-0.14856270173162045
1201	2	-0.044487587801626205
1201	3	-0.021631314459540363
1201	4	-0.19415919132772075
1201	5	0.23112688033461382
1201	6	-0.3950522298192496
1201	7	0.4998838203769367
1201	8	-0.11566794290492055
1201	9	-0.06539369659872563
1201	10	-0.29501152475273074
1201	11	-0.21107347479006933
1201	12	-0.20004158834492358
1201	13	-0.10200861972874975
1201	14	-0.06598618406975731
1201	15	-0.13397723779498705
1201	16	-0.13691569693617822
1201	17	-0.31979773212385965
1201	18	-0.08148123111365635
1201	19	-0.045750686360911175
1201	20	-1.1569192289908514
1201	21	-0.48574585799997294
1201	22	-0.48907893620254583
1201	23	-0.15471739540620888
1201	24	-0.35554031513251516
1201	25	-0.6014431002010099
1201	26	-0.5743406753433504
1201	27	-0.03350573327990247
1201	28	-1.2720048380454756
1201	29	-0.38363699809619334
1201	30	-1.2032939755547467
1201	31	-0.7035005459243209
1201	32	-1.3327414327903213
1201	33	0.15716018718475055
1201	34	-1.2150770598141705
1201	35	-0.94296176494462
1201	36	-0.7932372373156156
1201	37	-1.4229495114422832
1201	38	-2.045757642813907
1201	39	-1.7493328575134512
1201	40	-0.7038328091656254
1201	41	-0.2677528277465484
1201	42	-0.6407607839828486
1201	43	-0.851663008101859
1201	44	-0.8433150986930258
1201	45	-0.8208647419240904
1201	46	-0.5092012139539506
1201	47	1.1940963526712451
1201	48	0.6023976900737189
1201	49	-1.5923217408567787
1201	50	-1.2966672586679806
1201	51	-1.25932065078225
1201	52	-0.762410588676218
1201	53	-0.581556603502225
1201	54	-1.3765987214180058
1201	55	-0.8588540776434321
1201	56	-1.1406710216703004
1201	57	-0.15374020811314892
1201	58	-1.4176638486776305
1201	59	-0.9075443254721414
1201	60	-0.6207257502959074
1201	61	-0.7671475842658069
1202	1	-0.5606060578135157
1202	2	-0.4770849382632889
1202	3	-0.08543433868006645
1202	4	-0.3623379682580037
1202	5	-0.4898151777411286
1202	6	-0.19497194077870716
1202	7	-0.2003436652379449
1202	8	-0.43441543387240245
1202	9	-0.5894135012287265
1202	10	-0.18967230355642783
1202	11	0.11757633117808552
1202	12	-8.712417639031855E-4
1202	13	-0.29454173895797453
1202	14	0.054982637019499236
1202	15	0.010810665322907198
1202	16	-0.19215455683180238
1202	17	-0.15954080719411223
1202	18	-0.2254662438260837
1202	19	-0.2139294632911941
1202	20	-0.7237866182588846
1202	21	0.040789669900469505
1202	22	-0.25249389672709555
1202	23	-0.35340600774396336
1202	24	-0.2645460691804189
1202	25	-0.6108101549313716
1202	26	-0.05069555290256034
1202	27	-0.27694210421526577
1202	28	-0.9312046239413689
1202	29	-0.3918800062589085
1202	30	-1.0663743984103604
1202	31	-0.4026307479850715
1202	32	-0.33779964234471294
1202	33	-0.7854331488248789
1202	34	-0.5703025382030376
1202	35	-0.28967660503561277
1202	36	0.006441606522198384
1202	37	-0.8244214771856235
1202	38	-1.8940648822090616
1202	39	-0.4271329378024902
1202	40	-0.06885355050109965
1202	41	0.03606090167938447
1202	42	-0.9886264336550035
1202	43	-0.5129503090519449
1202	44	0.11977870566934712
1202	45	-0.763913049163488
1202	46	-0.2201071419614422
1202	47	0.2838862630151482
1202	48	0.8187498913315778
1202	49	0.22386988231998828
1202	50	0.33705413836132436
1202	51	0.5441853039882574
1202	52	-0.0346171991405555
1202	53	0.30526261234147967
1202	54	0.518650843683023
1202	55	-0.0670970858061537
1202	56	0.1960344513659642
1202	57	0.7167000434591806
1202	58	0.27498970411241075
1202	59	0.7040172963664766
1202	60	0.9472656855397688
1202	61	0.14391892182277743
1203	1	-0.09479247410790492
1203	2	-0.01096848486736235
1203	3	0.23964579559216048
1203	4	0.21806131495039693
1203	5	-0.20112988458850367
1203	6	0.015825363681052806
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2403	34	0.3115023892687283
2403	35	-0.2163823158617184
2403	36	0.022751192110455065
2403	37	0.35718328719028847
2403	38	0.8181697781314625
2403	39	-0.061501682705745964
2403	40	0.06997561894560889
2403	41	-0.3962913853693202
2403	42	0.9607644280347678
2403	43	0.49725553768737285
2403	44	-0.49262152167639073
2403	45	-0.27867816641952103
2403	46	-0.04895533925800777
2403	47	-0.6511472210582916
2403	48	-1.2114475535000118
2403	49	0.43179238707695333
2403	50	1.5095650731971162
2403	51	0.2534792966242314
2403	52	0.4917870314670978
2403	53	0.09600568694547817
2403	54	0.28893280182175474
2403	55	0.17497670271288612
2403	56	0.09911597458241062
2403	57	-1.4900363096186382
2403	58	0.3640993490082529
2403	59	-1.9597828370755863
2403	60	-1.185632725511212
2403	61	0.12489107196981308
2404	1	0.08319188689628021
2404	2	-0.027236913947177754
2404	3	0.05004112810238581
2404	4	-0.13480865866182512
2404	5	0.5528827524947957
2404	6	-0.06847027628311508
2404	7	0.10563404499508522
2404	8	0.27914658668049697
2404	9	-0.024063957155782134
2404	10	0.18867477995782878
2404	11	0.06257560748434798
2404	12	0.13865690992230384
2404	13	-0.17614544426989198
2404	14	-0.2426002259129092
2404	15	-0.195586787275154
2404	16	0.17606081442308152
2404	17	0.03846179810332728
2404	18	-0.060174595251758944
2404	19	0.0952324993662117
2404	20	0.05519731574025144
2404	21	0.1285013503173147
2404	22	-0.061149273771533785
2404	23	-0.03324390034781288
2404	24	0.2287235791044253
2404	25	0.19075302353150647
2404	26	0.2448609675779707
2404	27	-0.23815212013653309
2404	28	1.078348133331751
2404	29	0.33036633908773894
2404	30	0.37088135495481467
2404	31	0.8340171026445375
2404	32	0.35926216110006237
2404	33	0.520399822661588
2404	34	0.020810502758028093
2404	35	0.8398645731377847
2404	36	0.6432920913970008
2404	37	-0.9213964373824695
2404	38	0.22085768256152605
2404	39	-0.789558759194879
2404	40	-0.27148779568853953
2404	41	-0.11157261981431787
2404	42	0.617973653188244
2404	43	0.1667428448053414
2404	44	-0.5231508065610768
2404	45	1.2627188802040052
2404	46	1.0842784420594
2404	47	-0.5489404847052164
2404	48	-0.6370324215935916
2404	49	-0.8590654194602969
2404	50	1.1412226142623254
2404	51	-0.648461967686373
2404	52	0.7197611479429575
2404	53	-0.07887402103527563
2404	54	-0.9365525142558973
2404	55	0.23338055205750408
2404	56	-0.47729019764257785
2404	57	-1.6778577796700738
2404	58	-0.3679398081180132
2404	59	0.07671956876479769
2404	60	0.25223022454686017
2404	61	-0.45848374137015924
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	12.0
16	70.0
17	154.0
18	170.0
19	200.0
20	211.0
21	286.0
22	365.0
23	543.0
24	921.0
25	1301.0
26	2131.0
27	4428.0
28	9424.0
29	11857.0
30	11684.0
31	7193.0
32	3630.0
33	1907.0
34	1980.0
35	2701.0
36	3252.0
37	4281.0
38	5710.0
39	11179.0
40	2963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.2805501914151165	67.10144436539396	12.169258393468171	13.448747049722758
2	50.87461746072973	5.954626043160593	27.645590776145358	15.525165719964315
3	7.041060619748849	49.969509440780556	11.796458578010661	31.192971361459932
4	6.937088523257258	6.789154517633508	71.65765135003896	14.616105609070274
5	5.694894582905153	7.190044380201687	12.760719569071629	74.35434146782153
6	50.871229659074224	7.036464038485427	26.781701353991394	15.310604948448953
7	50.30546678260477	8.35996521856967	11.082628482377785	30.251939516447777
8	7.3605637301954765	51.93274084446603	26.617957607308618	14.08873781802988
9	7.62255372488792	54.063668085779135	10.128397682743667	28.185380506589276
10	51.224690298465326	25.559834223572324	9.03865481688932	14.17682066107303
11	8.696486849683241	67.3167481621176	8.982191455964225	15.004573532234932
12	52.521089065305524	10.74949465291972	8.882815940736057	27.8466003410387
13	8.98444999040123	25.4265806917891	9.505042178130612	56.083927139679055
14	53.218976206339704	24.241979379580588	9.52762752250065	13.011416891579056
15	8.961864646031191	10.993416372116133	53.110566553363526	26.934152428489156
16	9.706051743023952	25.09344686233103	9.95223199665737	55.24826939798765
17	53.85249511591928	23.990152789854662	9.567151875148216	12.59020021907784
18	10.556389958555894	10.754011721793729	53.41433943514053	25.275258884509842
19	54.65992117714815	24.83258613485709	9.883346696328752	10.624145991666008
20	11.979266653868304	25.137488283852612	51.45280227660272	11.430442785676375
21	13.596377310763048	53.504680812620684	8.441272458301807	24.457669418314456
22	71.45438325070862	10.676092283717097	8.081036215599697	9.788488249974591
23	70.51144512325952	12.983185211116508	7.946653416597969	8.558716249026007
24	57.8128352512055	25.67614874707802	7.954558287127483	8.556457714589003
25	29.87363499824964	53.63567580996691	8.072002077851682	8.41868711393177
26	27.897417365871284	55.90663218637426	7.885672986798867	8.310277460955586
27	14.729032330920466	68.27662529784423	8.458211466579337	8.536130904655968
28	30.219190767111222	53.54194663083125	7.676758551376012	8.562104050681512
29	29.408376904226845	55.41540094632593	7.808882815940736	7.3673393335064885
30	17.884204939414815	66.44834167108962	7.896965658983886	7.770487730511672
31	30.969024200196493	12.135105530021569	49.765677052160854	7.130193217621085
32	73.0376158910483	12.35982970650345	8.06974354341468	6.532810859033574
33	16.263890143644414	26.641973077965492	50.77129822025477	6.322838558135333
34	30.390839384323513	27.184849750996577	36.48775309701534	5.936557767664563
35	17.395232233803483	65.41167436450486	8.319311598703601	8.873781802988042
36	19.153501293010965	26.407913904667264	43.46436597292017	10.9742188294016
37	32.36931555113887	13.35132632434813	6.640091244791255	47.639266879721745
38	18.669256482067034	28.76163158370223	5.746905772879212	46.822206161351524
39	32.770205413707046	46.28979255361196	4.84342710015471	16.096574932526284
40	21.666120854177723	62.96229376757422	5.175431662394272	10.196153715853782
41	68.7938296839181	20.74915587275417	5.942204103757072	4.514810339570652
42	59.87487719218999	28.450758302937224	7.301841834833376	4.3725226700394115
43	31.666497272758072	14.93941344535917	49.051958758229716	4.342130523653036
44	25.931363138459453	28.09390986189062	40.993529298837984	4.981197700811943
45	17.685453908958475	25.70099262588506	50.21851320678012	6.395040258376341
46	31.631489198315094	11.406985804790459	8.180596492416798	48.78092850447764
47	25.8997436563414	24.505098641491536	9.194493693042585	40.40066400912448
48	15.878626359355414	51.762221494472236	23.553126376294422	8.806025769877927
49	17.520580895057197	10.64898987047305	9.577315280114734	62.25311395435502
50	29.951554436326266	11.331067270448205	10.216480525786817	48.50089776743871
51	28.77372872742877	13.43940916739128	11.45189886282791	46.33496324235204
52	14.995708736109856	27.462959616948236	8.706748577107236	48.83458306983467
53	16.872572048062157	23.63808835486494	6.801653265877676	52.68768633119522
54	33.610759779564546	11.121149155298582	7.079456138765923	48.188634926370945
55	16.83756436895835	24.639759689222153	48.74762851206071	9.775047429758786
56	29.145257642315904	12.24238591577925	50.12930109651846	8.483055345386378
57	47.7933869364893	14.03920860059626	17.300569157105432	20.86683530580902
58	25.31732767187641	14.135197398138946	52.98807480350528	7.559400126479357
59	11.337970909747945	14.347502032703948	23.92831330743518	50.38621375011293
60	11.056905060360696	11.41827873203013	45.02490090456347	32.499915303045704
61	23.17960068660222	13.166275182943355	11.529948504833317	52.124175625621106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.5
13	1.0
14	2.5
15	4.0
16	7.0
17	14.0
18	18.0
19	31.0
20	49.5
21	55.0
22	56.0
23	57.0
24	62.0
25	62.5
26	58.0
27	62.0
28	66.0
29	59.5
30	89.0
31	125.0
32	85.5
33	46.0
34	57.0
35	81.0
36	94.0
37	98.5
38	103.0
39	130.5
40	157.5
41	157.0
42	238.5
43	502.5
44	685.0
45	1202.5
46	1720.0
47	3996.0
48	15916.0
49	25560.0
50	21756.5
51	17953.0
52	15561.0
53	10588.0
54	8007.0
55	6419.0
56	4831.0
57	3970.0
58	2477.5
59	1846.0
60	1534.0
61	1010.0
62	798.0
63	708.0
64	618.0
65	520.5
66	364.5
67	306.0
68	244.0
69	182.0
70	160.5
71	103.5
72	68.0
73	49.0
74	30.0
75	26.0
76	16.0
77	10.0
78	7.5
79	5.0
80	3.0
81	1.5
82	2.0
83	2.0
84	2.5
85	3.0
86	2.5
87	2.0
88	1.0
89	1.5
90	3.0
91	2.5
92	2.0
93	4.5
94	4.0
95	1.0
96	2.0
97	3.0
98	5.0
99	23.5
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002258534437003828
2	0.0
3	0.001129267218501914
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.001129267218501914
34	0.0
35	0.0
36	0.0
37	0.0
38	0.001129267218501914
39	0.0
40	0.0
41	0.0
42	0.0
43	0.002258534437003828
44	0.0
45	0.0
46	0.002258534437003828
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.001129267218501914
53	0.001129267218501914
54	0.001129267218501914
55	0.001129267218501914
56	0.0
57	0.001129267218501914
58	0.001129267218501914
59	0.001129267218501914
60	0.002258534437003828
61	0.001129267218501914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	88553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.036768940634424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.7782218251916	44.567660045396536
2	3.770746156377827	3.622689236954141
3	1.0343692698293292	1.4906327284225267
4	0.47722036767125864	0.9169649814235542
5	0.28445154920306553	0.6832066671936581
6	0.22097888946353847	0.6369067112350796
7	0.15515539047439936	0.5217214549478844
8	0.12224364097982982	0.4697751628967963
9	0.1034369269829329	0.447189818526758
>10	0.8368987728619117	7.99408263977505
>50	0.11284028398138135	3.971632807471232
>100	0.07757769523719968	8.161214188113334
>500	0.014105035497672669	4.9620001580974105
>1k	0.009403356998448445	9.062369428477862
>5k	0.0	0.0
>10k+	0.0023508392496121113	12.491953971068174
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	11062	12.491953971068174	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCCACCCCCCTTGTCTC	3889	4.391720212753944	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCCACCCCCCTTGTCTC	1623	1.8328006956286065	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCCACCCCCCTTGTCCC	1288	1.4544961774304654	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTTTCCACCCCCCTTGTCTC	1225	1.3833523426648449	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCGACCCCCCTTGTCTC	928	1.0479599787697762	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCCACCCCCCTTGTCTC	892	1.0073063589037075	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCCACCCCCCTTTTCTC	779	0.8796991632129911	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCCACCCCCCTTTTCCC	631	0.7125676148747078	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTGTCCACCCCCCTTGTCTC	623	0.7035334771266926	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCGACCCCCCTTGTCTC	541	0.6109335652095356	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCCACCCCCCTTGTCCC	439	0.49574830892234034	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTACCCAAGGTTTCCACCCCCCTTGTCTC	384	0.433638611904735	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTTTCCACCCCCCTTTTCTC	329	0.37152891488712975	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTGTCGACCCCCCTTGTCTC	323	0.36475331157611823	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCACGGTTTCCACCCCCCTTGTCTC	320	0.3613655099206125	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCGACCCCCCTTGTCTC	313	0.3534606393910991	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCCACCCCCGTTGTCTC	307	0.3466850360800876	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTTTCCACCCCCCTTGTCCC	306	0.34555576886158573	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCCAGGTTTCCACCCCCCTTGTCTC	305	0.3444265016430838	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCCACCCCCCTTGTCCC	287	0.3240996917100494	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAAGTTTCCACCCCCCTTGTCTC	278	0.31393628674353213	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	253	0.28570460628098426	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTTTCGACCCCCCTTGTCTC	241	0.2721533996589613	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCCACCCCCCTTTTCTC	229	0.2586021930369384	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTTTCCACCCCCCTTTTCCC	226	0.2552143913814326	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCCGGTTTCCACCCCCCTTGTCTC	222	0.25069732250742494	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTGTCCACCCCCCTTGTCTC	203	0.22924124535588855	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTCCAAGGTTTCCACCCCCCTTGTCTC	201	0.22698271091888475	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGTGTA	196	0.22133637482637517	Illumina Single End PCR Primer 1 (100% over 21bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTGTCCACCCCCCTTGTCCC	192	0.2168193059523675	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCGACCCCCGTTGTCTC	183	0.2066559009858503	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	165	0.18632909105281584	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCCACCCCCGTTGTCTC	161	0.18181202217880818	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAAGTTTCCACCCCCCTTGTCTC	135	0.1524510744977584	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTACCCAAGGTTTCCACCCCCCTTGTCCC	131	0.14793400562375075	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAAGTTTCCACCCCCCTTGTCTC	118	0.13325353178322588	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCCACCCCCCTTTTCTC	118	0.13325353178322588	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCCACCCCCGTTGTCTC	115	0.12986573012772012	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTACCCCAGGTTTCCACCCCCCTTGTCTC	114	0.1287364629092182	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCGACCCCCGTTGTCTC	113	0.1276071956907163	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCCACCCCCCTTTTCCC	109	0.12309012681670864	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTACCCAAGGTTTCGACCCCCCTTGTCTC	106	0.1197023251612029	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTGTCGACCCCCGTTGTCTC	105	0.118573057942701	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCGACCCCCGTTGTCTC	100	0.1129267218501914	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGACCCAAGGTTTCCACCCCCCTTGTCTC	100	0.1129267218501914	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAGGGTTTCCACCCCCCTTGTCTC	99	0.1117974546316895	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTATCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	99	0.1117974546316895	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCACGGTTTCCACCCCCCTTGTCCC	96	0.10840965297618375	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGATCGGAAGAGCGTCGTGT	95	0.10728038575768184	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCCACCCCCCTTTTCCC	95	0.10728038575768184	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAGATCGGAAGAGCGTCGT	94	0.10615111853917994	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTCCAAGGTTTCCACCCCCCTTTTCCC	93	0.10502185132067801	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTACCCAAAGTTTCCACCCCCCTTGTCTC	91	0.1027633168836742	Illumina Single End PCR Primer 1 (96% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	43.32433683782593	0.0	0.0	0.0	0.0
2	43.49824398947523	0.0	0.0	0.0	0.0
3	43.56600002258534	0.0	0.0	0.0	0.0
4	43.63601459013246	0.0	0.0	0.0	0.0
5	43.75797544973067	0.0	0.0	0.0	0.0
6	43.94204600634648	0.0	0.0	0.0	0.0
7	44.17241651892087	0.0	0.0	0.0	0.0
8	44.69187943943175	0.0	0.0	0.0	0.0
9	45.03291813941933	0.0	0.0	0.0	0.0
10	45.5444761894007	0.0	0.0	0.0	0.0
11	46.14863415129922	0.0	0.0	0.0	0.0
12	46.90976025656951	0.0	0.0	0.0	0.0
13	47.88657640057367	0.0	0.0	0.0	0.0
14	48.97293146477251	0.0	0.0	0.0	0.0
15	50.3811276862444	0.0	0.0	0.0	0.0
16	51.916931103407	0.0	0.0	0.0	0.0
17	53.13653969938907	0.0	0.0	0.0	0.0
18	54.501823766557884	0.0	0.0	0.0	0.0
19	55.5159057287726	0.0	0.0	0.0	0.0
20	56.673404627737064	0.0	0.0	0.0	0.0
21	57.773310898557924	0.0	0.0	0.0	0.0
22	58.51975652998769	0.0	0.0	0.0	0.0
23	59.56207017266496	0.0	0.0	0.0	0.0
24	60.57050579878717	0.0	0.0	0.001129267218501914	0.0
25	61.253712465980826	0.0	0.0	0.001129267218501914	0.0
26	62.148092103034344	0.0	0.0	0.001129267218501914	0.0
27	62.96794010366673	0.001129267218501914	0.0	0.001129267218501914	0.0
28	63.95943672151141	0.001129267218501914	0.0	0.001129267218501914	0.0
29	65.05030885458426	0.002258534437003828	0.0	0.001129267218501914	0.0
30	66.39865391347554	0.002258534437003828	0.0	0.001129267218501914	0.0
31	67.72102582634129	0.0033878016555057423	0.0	0.001129267218501914	0.0
32	69.07840502298059	0.006775603311011485	0.0	0.001129267218501914	0.0
33	70.51144512325952	0.006775603311011485	0.0	0.001129267218501914	0.0
34	71.83155850168825	0.009034137748015312	0.0	0.001129267218501914	0.0
35	72.96647205628268	0.01355120662202297	0.0	0.001129267218501914	0.0
36	73.91279798538729	0.01693900827752871	0.0	0.001129267218501914	0.0
37	74.66714848734657	0.01919754271453254	0.0	0.001129267218501914	0.0
38	75.1041749009068	0.03049021489955168	0.0	0.001129267218501914	0.0
39	75.48699648797896	0.035007283773559335	0.0	0.001129267218501914	0.0
40	75.82464738631103	0.03726581821056316	0.0	0.001129267218501914	0.0
41	76.5756100866148	0.03726581821056316	0.0	0.001129267218501914	0.0
42	77.09281447268867	0.03839508542906508	0.0	0.001129267218501914	0.0
43	77.49257506803835	0.03839508542906508	0.0	0.001129267218501914	0.0
44	78.87253960904769	0.03839508542906508	0.0	0.001129267218501914	0.0
45	92.88787505787495	0.03839508542906508	0.0	0.001129267218501914	0.0
46	93.32151366977968	0.03839508542906508	0.0	0.001129267218501914	0.0
47	93.4558964687814	0.03839508542906508	0.0	0.001129267218501914	0.0
48	93.61060607771617	0.03839508542906508	0.0	0.001129267218501914	0.0
49	93.75176448002891	0.03839508542906508	0.0	0.001129267218501914	0.0
50	93.86243266744209	0.03839508542906508	0.0	0.001129267218501914	0.0
>>END_MODULE