AM-AL-EC06_S56_R2_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	AM-AL-EC06_S56_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88553
Sequences flagged as poor quality	0
Sequence length	61
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	11062	12.491953971068174	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCCACCCCCCTTGTCTC	3889	4.391720212753944	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCCACCCCCCTTGTCTC	1623	1.8328006956286065	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCCACCCCCCTTGTCCC	1288	1.4544961774304654	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTTTCCACCCCCCTTGTCTC	1225	1.3833523426648449	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCGACCCCCCTTGTCTC	928	1.0479599787697762	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCCACCCCCCTTGTCTC	892	1.0073063589037075	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCCACCCCCCTTTTCTC	779	0.8796991632129911	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCCACCCCCCTTTTCCC	631	0.7125676148747078	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTGTCCACCCCCCTTGTCTC	623	0.7035334771266926	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCGACCCCCCTTGTCTC	541	0.6109335652095356	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCCACCCCCCTTGTCCC	439	0.49574830892234034	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTACCCAAGGTTTCCACCCCCCTTGTCTC	384	0.433638611904735	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTTTCCACCCCCCTTTTCTC	329	0.37152891488712975	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTGTCGACCCCCCTTGTCTC	323	0.36475331157611823	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCACGGTTTCCACCCCCCTTGTCTC	320	0.3613655099206125	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCGACCCCCCTTGTCTC	313	0.3534606393910991	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCCACCCCCGTTGTCTC	307	0.3466850360800876	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTTTCCACCCCCCTTGTCCC	306	0.34555576886158573	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCCAGGTTTCCACCCCCCTTGTCTC	305	0.3444265016430838	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCCACCCCCCTTGTCCC	287	0.3240996917100494	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAAGTTTCCACCCCCCTTGTCTC	278	0.31393628674353213	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	253	0.28570460628098426	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTTTCGACCCCCCTTGTCTC	241	0.2721533996589613	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCCACCCCCCTTTTCTC	229	0.2586021930369384	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTTTCCACCCCCCTTTTCCC	226	0.2552143913814326	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCCGGTTTCCACCCCCCTTGTCTC	222	0.25069732250742494	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAGGTGTCCACCCCCCTTGTCTC	203	0.22924124535588855	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTCCAAGGTTTCCACCCCCCTTGTCTC	201	0.22698271091888475	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGTGTA	196	0.22133637482637517	Illumina Single End PCR Primer 1 (100% over 21bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTGTCCACCCCCCTTGTCCC	192	0.2168193059523675	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTTTCGACCCCCGTTGTCTC	183	0.2066559009858503	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	165	0.18632909105281584	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCCACCCCCGTTGTCTC	161	0.18181202217880818	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAAGTTTCCACCCCCCTTGTCTC	135	0.1524510744977584	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTACCCAAGGTTTCCACCCCCCTTGTCCC	131	0.14793400562375075	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCCAAGTTTCCACCCCCCTTGTCTC	118	0.13325353178322588	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCCACCCCCCTTTTCTC	118	0.13325353178322588	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCCACCCCCGTTGTCTC	115	0.12986573012772012	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTACCCCAGGTTTCCACCCCCCTTGTCTC	114	0.1287364629092182	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCGACCCCCGTTGTCTC	113	0.1276071956907163	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCCACCCCCCTTTTCCC	109	0.12309012681670864	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTACCCAAGGTTTCGACCCCCCTTGTCTC	106	0.1197023251612029	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTGTCGACCCCCGTTGTCTC	105	0.118573057942701	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGATCCAAGGTTTCGACCCCCGTTGTCTC	100	0.1129267218501914	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTGACCCAAGGTTTCCACCCCCCTTGTCTC	100	0.1129267218501914	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAGGGTTTCCACCCCCCTTGTCTC	99	0.1117974546316895	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTATCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	99	0.1117974546316895	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCACGGTTTCCACCCCCCTTGTCCC	96	0.10840965297618375	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGATCGGAAGAGCGTCGTGT	95	0.10728038575768184	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTATCCAAGGTGTCCACCCCCCTTTTCCC	95	0.10728038575768184	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAGATCGGAAGAGCGTCGT	94	0.10615111853917994	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTCCAAGGTTTCCACCCCCCTTTTCCC	93	0.10502185132067801	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTACCCAAAGTTTCCACCCCCCTTGTCTC	91	0.1027633168836742	Illumina Single End PCR Primer 1 (96% over 29bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content