##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-EC06_S56_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88553
Sequences flagged as poor quality	0
Sequence length	61
%GC	55
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.31173421566746	40.0	40.0	40.0	40.0	40.0
2	39.13242916671372	40.0	40.0	40.0	40.0	40.0
3	38.99725588065904	40.0	40.0	40.0	40.0	40.0
4	38.82556209275801	40.0	40.0	40.0	40.0	40.0
5	39.281334342145385	40.0	40.0	40.0	40.0	40.0
6	39.088048965026594	40.0	40.0	40.0	40.0	40.0
7	39.217959865843056	40.0	40.0	40.0	40.0	40.0
8	39.06948381195442	40.0	40.0	40.0	40.0	40.0
9	39.26082684945739	40.0	40.0	40.0	40.0	40.0
10	39.259968606371324	40.0	40.0	40.0	40.0	40.0
11	39.35884724402335	40.0	40.0	40.0	40.0	40.0
12	39.383736293519135	40.0	40.0	40.0	40.0	40.0
13	39.00394114259257	40.0	40.0	40.0	40.0	40.0
14	39.37470215577112	40.0	40.0	40.0	40.0	40.0
15	39.45777105236412	40.0	40.0	40.0	40.0	40.0
16	39.1064334353438	40.0	40.0	40.0	40.0	40.0
17	38.92389868214516	40.0	40.0	40.0	40.0	40.0
18	39.3852720969363	40.0	40.0	40.0	40.0	40.0
19	38.619628922792	40.0	40.0	40.0	40.0	40.0
20	38.88993032421262	40.0	40.0	40.0	40.0	40.0
21	39.38793716757196	40.0	40.0	40.0	40.0	40.0
22	38.2172484274954	40.0	40.0	40.0	24.0	40.0
23	38.11787291226723	40.0	40.0	40.0	24.0	40.0
24	39.21850191410793	40.0	40.0	40.0	40.0	40.0
25	39.28616760584057	40.0	40.0	40.0	40.0	40.0
26	39.213307284902825	40.0	40.0	40.0	40.0	40.0
27	39.15250753785868	40.0	40.0	40.0	40.0	40.0
28	38.98578252571906	40.0	40.0	40.0	40.0	40.0
29	39.133942384786515	40.0	40.0	40.0	40.0	40.0
30	39.07585287906677	40.0	40.0	40.0	40.0	40.0
31	39.101238806138696	40.0	40.0	40.0	40.0	40.0
32	39.02083498018136	40.0	40.0	40.0	40.0	40.0
33	38.91574537282757	40.0	40.0	40.0	40.0	40.0
34	38.92091741668831	40.0	40.0	40.0	40.0	40.0
35	39.046717784829426	40.0	40.0	40.0	40.0	40.0
36	38.921549806330674	40.0	40.0	40.0	40.0	40.0
37	38.85447133355166	40.0	40.0	40.0	40.0	40.0
38	38.91649068919178	40.0	40.0	40.0	40.0	40.0
39	37.52807922938805	40.0	40.0	40.0	40.0	40.0
40	38.93234560093955	40.0	40.0	40.0	40.0	40.0
41	38.69501880229919	40.0	40.0	40.0	40.0	40.0
42	38.92493760798618	40.0	40.0	40.0	40.0	40.0
43	38.88591013291475	40.0	40.0	40.0	40.0	40.0
44	38.99029959459307	40.0	40.0	40.0	40.0	40.0
45	37.8669497363161	40.0	40.0	40.0	24.0	40.0
46	37.87512563097806	40.0	40.0	40.0	24.0	40.0
47	33.247478910934696	40.0	24.0	40.0	24.0	40.0
48	38.48745948753854	40.0	40.0	40.0	40.0	40.0
49	37.83496888868813	40.0	40.0	40.0	24.0	40.0
50	37.942972005465656	40.0	40.0	40.0	24.0	40.0
51	37.164681038474136	40.0	40.0	40.0	24.0	40.0
52	37.325533861077545	40.0	40.0	40.0	24.0	40.0
53	36.0120605738936	40.0	40.0	40.0	24.0	40.0
54	34.32150237710749	40.0	24.0	40.0	24.0	40.0
55	34.567818142807134	40.0	24.0	40.0	24.0	40.0
56	34.119634569128095	40.0	24.0	40.0	24.0	40.0
57	31.35058100798392	40.0	24.0	40.0	12.0	40.0
58	29.572527187108285	40.0	24.0	40.0	12.0	40.0
59	27.500999401488375	24.0	12.0	40.0	12.0	40.0
60	27.419375966935057	24.0	12.0	40.0	12.0	40.0
61	26.037107720799973	24.0	12.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.16937729253048417
1101	2	0.409462969609784
1101	3	0.38018853228659566
1101	4	-0.8761332931928365
1101	5	0.10359470421261108
1101	6	0.2976959411513036
1101	7	-0.16378708857806146
1101	8	0.36646101125391084
1101	9	0.18600051268480655
1101	10	0.07075186643847076
1101	11	0.11137892430445362
1101	12	0.2979351971758959
1101	13	0.07474099903547682
1101	14	0.036039435699876776
1101	15	0.04984927655149107
1101	16	0.1479252563923481
1101	17	0.3910113046225945
1101	18	0.08579522323547195
1101	19	0.4388134619987696
1101	20	-0.02434515196954834
1101	21	0.24519596886635497
1101	22	-0.14951284910089413
1101	23	0.5165489056265997
1101	24	-0.012221034069888503
1101	25	0.09426091523844349
1101	26	-0.27871323837896966
1101	27	-0.7858703513747543
1101	28	-0.7195189803486315
1101	29	-0.01187872568397097
1101	30	-0.32391578559315093
1101	31	-0.3297484061322322
1101	32	0.09621742379083997
1101	33	-0.2845664682019162
1101	34	-0.45380819311237985
1101	35	-0.36307634206462325
1101	36	-0.6370637126584384
1101	37	-0.18896740128077738
1101	38	-0.816896345054559
1101	39	1.0787003957278642
1101	40	-0.31311435729575265
1101	41	-0.5858162932029316
1101	42	-0.7824242281209521
1101	43	0.015818617134073065
1101	44	-0.7668250540996127
1101	45	-0.1595396769829165
1101	46	-0.6646264469480698
1101	47	0.14570621414588203
1101	48	-0.5043813064065858
1101	49	-0.5409472558994253
1101	50	-0.6436551816216465
1101	51	-0.7288426772736543
1101	52	-0.2526023150642871
1101	53	-0.06323601887623198
1101	54	-0.8960253471439827
1101	55	-0.6852062957344245
1101	56	-0.9429262124866469
1101	57	0.29339995960648224
1101	58	0.8415547958846012
1101	59	0.9565736050510907
1101	60	1.232196421254649
1101	61	1.0599678708944538
1102	1	0.23820584758399832
1102	2	0.3329571783613048
1102	3	-0.042485971558690494
1102	4	-0.899005059357016
1102	5	-0.16398896566623478
1102	6	-0.22835468062485376
1102	7	0.09601271682532087
1102	8	-0.01724085607384751
1102	9	-0.1842128771615137
1102	10	-0.12416463028937841
1102	11	-0.20338775968931344
1102	12	0.04463971688213775
1102	13	-0.06254291208984597
1102	14	0.09137951327194571
1102	15	0.27451506353484234
1102	16	-0.08223188703602347
1102	17	-0.214477386115405
1102	18	-0.24395273416653396
1102	19	-0.539820896298913
1102	20	-0.03730048804856523
1102	21	-0.3092710896592479
1102	22	-0.5416916647272672
1102	23	-0.4382007178422853
1102	24	-0.13073836782973558
1102	25	0.0963934808481568
1102	26	0.13015290314341854
1102	27	0.11961700650974194
1102	28	-0.31075946710673463
1102	29	-0.09819431873713569
1102	30	0.19205846367711388
1102	31	0.41249105432866173
1102	32	-0.017821522188604888
1102	33	0.6352090792676677
1102	34	0.4157454342484428
1102	35	-0.029169881723682067
1102	36	-0.18559957308202257
1102	37	0.03479204531844715
1102	38	-0.2530993119864675
1102	39	1.064198949581808
1102	40	0.42160780939091325
1102	41	0.37282526250368164
1102	42	0.23390322732841895
1102	43	0.1549685231679021
1102	44	0.03224727986016518
1102	45	-0.14498491669662172
1102	46	0.03837380629409637
1102	47	-0.041319789826026465
1102	48	-0.33244320412340755
1102	49	0.20625041960406065
1102	50	0.0758191409555593
1102	51	0.06164608010702466
1102	52	0.7537553961596686
1102	53	-0.20862367931847103
1102	54	-0.5263984128403365
1102	55	-0.28929549029105317
1102	56	-1.2783787828946345
1102	57	-0.19964920935935737
1102	58	-0.35598742225020175
1102	59	-0.5267857189256127
1102	60	-0.27957933947148916
1102	61	-0.8934089134631797
1103	1	0.3603885284855366
1103	2	0.28692592352608415
1103	3	0.35820514195928865
1103	4	0.3811537936008449
1103	5	-0.049800508293493806
1103	6	0.14283516929004492
1103	7	0.21432722505164037
1103	8	0.2695712538854096
1103	9	0.14659325002400436
1103	10	-0.03818804562796885
1103	11	0.20142940468223713
1103	12	0.0917798478842542
1103	13	0.12049901890195258
1103	14	0.459423249410996
1103	15	-0.0639758333654612
1103	16	-0.028593225939523848
1103	17	-0.10776739591477735
1103	18	-0.05652687414280422
1103	19	-0.47292729683530155
1103	20	-0.058704376851494544
1103	21	-0.09285972238914297
1103	22	-0.6401495532207448
1103	23	-0.6404287853032216
1103	24	-0.08875580039459408
1103	25	0.3751597916429361
1103	26	0.4081455794031541
1103	27	0.32437228011848873
1103	28	0.46416445883065194
1103	29	0.21837693300354033
1103	30	0.208407940184415
1103	31	-0.015793024272603873
1103	32	0.22551232690108236
1103	33	0.3236406641869465
1103	34	0.3037231535738272
1103	35	-0.07517534874106246
1103	36	0.61908349463679
1103	37	0.3042747416926801
1103	38	0.3605991768474084
1103	39	1.501921369510633
1103	40	0.3530637895858675
1103	41	0.7776424268752322
1103	42	0.3931172146408173
1103	43	0.22490508513602947
1103	44	0.37728828249051816
1103	45	0.0021603718690599294
1103	46	0.5387606235616147
1103	47	0.8804399708630299
1103	48	0.4601195892130221
1103	49	0.4170400425861658
1103	50	0.735110491721457
1103	51	0.27197152236809785
1103	52	0.15140354717313187
1103	53	-0.2877922800714785
1103	54	0.506352115809932
1103	55	1.184684601513581
1103	56	0.5745790798110235
1103	57	0.21914495227868613
1103	58	0.22960234514367883
1103	59	0.4525840268065018
1103	60	0.7479104743404648
1103	61	-0.035293487190550366
1104	1	0.1325707826821798
1104	2	0.08251553879039619
1104	3	-0.0397721283983401
1104	4	-0.591111872127442
1104	5	-0.3463285032888024
1104	6	-0.29789051103526276
1104	7	-0.21336540345425448
1104	8	-0.34423335460171955
1104	9	-0.050466493485537
1104	10	0.3270501985592915
1104	11	-0.313324009977336
1104	12	-0.004977528823076227
1104	13	-0.05998268269329543
1104	14	0.014214199259811267
1104	15	-0.05949246353958415
1104	16	-0.35243849754832013
1104	17	0.04477144257964483
1104	18	-0.21000409236822293
1104	19	-0.4740742053954108
1104	20	0.3384387781896905
1104	21	0.11316676077215249
1104	22	0.23994637004064856
1104	23	0.0398965196701937
1104	24	-0.025154507515850355
1104	25	-0.42819752250574084
1104	26	-0.020798222077402784
1104	27	-0.06057193841972719
1104	28	-0.3239702507929536
1104	29	-0.0650137457577955
1104	30	0.12697743241671589
1104	31	0.1487362218956818
1104	32	0.13000612316842108
1104	33	-0.3762863507228644
1104	34	0.3231163346811172
1104	35	-0.11125083617719866
1104	36	-0.15518404785095896
1104	37	0.2384838961082778
1104	38	-0.1378889891415369
1104	39	0.7753026644746583
1104	40	-0.33289179377353406
1104	41	-0.19754264246059705
1104	42	-0.24967290109268703
1104	43	-0.14293976941511488
1104	44	-0.03559879914851649
1104	45	-0.7738796656661293
1104	46	-0.28513678025446154
1104	47	-0.21454491079686022
1104	48	-0.13658565528703548
1104	49	0.27138265545239193
1104	50	0.0973250678866151
1104	51	-0.22745502335184398
1104	52	0.05537602136768527
1104	53	0.43486437721139026
1104	54	0.6441896718520539
1104	55	-0.5105505147412401
1104	56	0.369282447551619
1104	57	-0.8134898094771295
1104	58	-0.1711900644069395
1104	59	-0.02108920851827989
1104	60	-1.0516933208842225
1104	61	-0.3074236091799136
1201	1	-0.21193851553962162
1201	2	-0.001589252847992384
1201	3	0.26955324149442106
1201	4	-0.18264166715933783
1201	5	0.21960064018886527
1201	6	0.021588245582087495
1201	7	0.17637261744977906
1201	8	0.22620237228846918
1201	9	0.07604361917905322
1201	10	0.15634299074064018
1201	11	-0.043634855640426906
1201	12	0.2802403346220146
1201	13	0.36939155376659016
1201	14	-0.20954038198085811
1201	15	-0.18041006907779433
1201	16	-0.08459541352497268
1201	17	-0.2935480904731591
1201	18	0.02146054724084223
1201	19	0.5978410925549085
1201	20	-0.18410771291930672
1201	21	-0.3004615818367782
1201	22	0.1686554782258014
1201	23	0.2040338890199962
1201	24	0.012201268240900731
1201	25	-0.10660121464636774
1201	26	-0.24145792073357342
1201	27	-0.0281571155610294
1201	28	-0.11297527319013057
1201	29	-0.06349345255839722
1201	30	-0.03118735650686233
1201	31	-0.1122655427303556
1201	32	-0.5441896145810077
1201	33	-0.05300686110700781
1201	34	-0.19307750270180435
1201	35	0.0952375580131104
1201	36	0.02985735299063208
1201	37	-0.3198980775580722
1201	38	-0.18208608429560513
1201	39	1.233996841208743
1201	40	0.08448298564821499
1201	41	0.5325781665525682
1201	42	0.15472508994844247
1201	43	0.35484766101789944
1201	44	0.18598393466443497
1201	45	0.381651758251337
1201	46	0.2509837572777798
1201	47	-0.34132650438991163
1201	48	0.09922267917747263
1201	49	-0.4255631841023799
1201	50	-0.09064832698239655
1201	51	0.49696468616355816
1201	52	-0.5020829171418839
1201	53	0.282237799228497
1201	54	-0.07007665093877335
1201	55	-0.23412863337840406
1201	56	-0.1805207287752495
1201	57	-0.6755774978170237
1201	58	0.08926873836216487
1201	59	-0.7917692682440851
1201	60	-1.1328661184951905
1201	61	-1.0915691229575728
1202	1	-0.08641998215276203
1202	2	-0.13320975331634344
1202	3	0.08900995232006181
1202	4	0.11565888148243886
1202	5	0.037772726365773224
1202	6	0.15856134875350847
1202	7	-0.11438076138067998
1202	8	0.05711365289866421
1202	9	0.1028066326943744
1202	10	-0.036029550407498334
1202	11	0.026591291823777397
1202	12	-0.06671537259061466
1202	13	-0.016102892908101296
1202	14	0.12146456917664494
1202	15	0.27552462916922593
1202	16	-0.220390944101716
1202	17	-0.7204716820695722
1202	18	-0.3260304202420983
1202	19	-0.43376602640668693
1202	20	-0.5011958970488379
1202	21	0.16184271362688918
1202	22	-0.3635538085398906
1202	23	0.024079386142972226
1202	24	0.24118561187799514
1202	25	-0.029095527505994312
1202	26	-0.17958183348614654
1202	27	-0.1615975009484245
1202	28	0.30351074313531257
1202	29	-0.08072883326226332
1202	30	-0.23100001541225623
1202	31	-0.0682136224841372
1202	32	0.3969585186988027
1202	33	0.2110905562621852
1202	34	0.29015546933084835
1202	35	0.016714876359152697
1202	36	-0.09630349272059391
1202	37	-0.7271776172342399
1202	38	-0.4469863920702579
1202	39	0.17568023475885752
1202	40	-0.2855424392146304
1202	41	-0.9716637710896094
1202	42	-0.34477979430152317
1202	43	-0.21049423647976084
1202	44	-0.17713663271145208
1202	45	-0.9312346327562864
1202	46	-0.03971609552564814
1202	47	-1.2089834025566475
1202	48	-0.9641788998403271
1202	49	-0.9924573363839215
1202	50	-1.1085527830241446
1202	51	-0.8646303894419489
1202	52	-0.7992594192160141
1202	53	-0.8172403220079545
1202	54	-1.1143694383066318
1202	55	-1.5168263451407498
1202	56	-1.3942769177221237
1202	57	-0.6787890594388593
1202	58	-1.0408262703358133
1202	59	-1.302889300359702
1202	60	-0.7303503952247503
1202	61	-0.9535790252725711
1203	1	-0.4593030492131547
1203	2	-0.1225588358735763
1203	3	-0.5264717366832414
1203	4	0.23978497623016892
1203	5	-0.3490423464491528
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2403	25	0.26250009361738336
2403	26	0.0880930621077951
2403	27	0.3850701541779742
2403	28	0.383823096410822
2403	29	0.13020847367919686
2403	30	0.4296370468854178
2403	31	0.3659673150699234
2403	32	0.1417876387225263
2403	33	0.3508587624548909
2403	34	0.3721976271384406
2403	35	0.12984409275770048
2403	36	0.4523410818710474
2403	37	0.28129842497299506
2403	38	0.5132528151850337
2403	39	1.2754405332737022
2403	40	0.25262446221122303
2403	41	0.20949618640661782
2403	42	0.4738625562557246
2403	43	0.5787908600186213
2403	44	0.49817905661565476
2403	45	0.09775194890283956
2403	46	0.6372305523602861
2403	47	0.28032887658438455
2403	48	0.3055470496988022
2403	49	0.4424402344762868
2403	50	0.5637728156117774
2403	51	0.9562112840203767
2403	52	0.3506157877427398
2403	53	0.09179667109754064
2403	54	-0.22969797761025745
2403	55	0.8083274839051384
2403	56	1.1045492863453887
2403	57	-0.3484458167967084
2403	58	-0.08681435939603688
2403	59	0.11026215638283077
2403	60	0.2963627290493491
2403	61	0.549769382071684
2404	1	0.36719124957397753
2404	2	0.15279400989106762
2404	3	0.018956398983831946
2404	4	0.06615823015808076
2404	5	0.06614152069201396
2404	6	-0.0023881374239422826
2404	7	-0.010458341348837052
2404	8	-0.0016502782056875276
2404	9	0.07501679335437927
2404	10	-0.3040857090411606
2404	11	-0.369703396264228
2404	12	-0.037767623278128326
2404	13	0.1355833134893487
2404	14	0.05835847567541208
2404	15	-0.1210595364118987
2404	16	0.11337660547130213
2404	17	0.16996108678264932
2404	18	0.14755475851025324
2404	19	0.1431626173912619
2404	20	0.3278676432609018
2404	21	-0.040212576574298
2404	22	0.48229942637109247
2404	23	0.4826117352528314
2404	24	0.003524590022443874
2404	25	-0.25749326958155194
2404	26	-0.09939656789054396
2404	27	-0.16246593342779647
2404	28	-0.3246554169457454
2404	29	0.03795693891895269
2404	30	-0.28050066673661433
2404	31	-0.1666359701466078
2404	32	-0.04304727085106208
2404	33	0.06779919716535687
2404	34	-0.04824372013217726
2404	35	-0.042049054745199044
2404	36	-0.26941103360928764
2404	37	0.016490062603651268
2404	38	-0.09932392282723868
2404	39	1.4679077640684568
2404	40	-0.11040855587405929
2404	41	0.016033435452577294
2404	42	0.12012105965708031
2404	43	-0.21165214645225916
2404	44	-0.3473493986689036
2404	45	0.0917788279471452
2404	46	0.5748446223785351
2404	47	0.0380856378254677
2404	48	0.130335501664959
2404	49	0.5380101697674746
2404	50	0.7632086875215194
2404	51	0.5795728237582196
2404	52	1.1709243989921205
2404	53	1.209102129866416
2404	54	0.9718948546445958
2404	55	0.7442823536579155
2404	56	1.0723608011952308
2404	57	1.5128450410098324
2404	58	0.7507499346142517
2404	59	2.040575745233003
2404	60	1.3104659313058598
2404	61	1.1182113930224062
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	1.0
17	6.0
18	22.0
19	19.0
20	41.0
21	53.0
22	74.0
23	114.0
24	164.0
25	244.0
26	364.0
27	425.0
28	527.0
29	675.0
30	831.0
31	890.0
32	1191.0
33	1417.0
34	2010.0
35	3300.0
36	9406.0
37	20236.0
38	14504.0
39	20839.0
40	11198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.405666662902442	54.698316262577215	27.41296172913397	8.483055345386378
2	56.245622754896864	8.511623703770644	10.333687278313716	24.909066263018776
3	11.813264372748524	53.33529072984541	25.581290300723857	9.270154596682213
4	11.035199259200706	8.509028491411923	54.893679491378045	25.56209275800933
5	11.694691314805823	8.721330728490283	25.472880647747676	54.11109730895621
6	57.39387711314128	9.378564249658398	9.2035278307906	24.024030806409723
7	56.75019479859519	10.165663500954231	25.28090522060235	7.803236479848226
8	7.918421736135421	65.39812315788285	4.897631926642801	21.785823179338927
9	19.61650085259675	52.8564814292006	22.283830022698268	5.243187695504387
10	52.56061341795309	19.012342890698225	22.33577631474936	6.091267376599325
11	8.428850518898287	50.92430521834381	34.00901155240364	6.6378327103542505
12	54.50295303377638	6.806093525911036	21.85244994523054	16.83850349508204
13	20.951294704866015	7.587546441114361	3.8530597495285313	67.60809910449109
14	19.520513139024086	54.12351924835974	3.9648572041602206	22.39111040845595
15	9.17303761589105	20.34261967409348	20.510880489650265	49.97346222036521
16	10.027892900296997	63.12039117816449	4.268630085937235	22.583085835601278
17	17.90679028378485	10.342958454259032	4.960870890878908	66.78938037107721
18	55.11953293507843	15.853782480548373	21.807279256490464	7.219405327882737
19	16.779781599719943	8.270753108308018	49.54321141011598	25.40625388185606
20	12.907524307476878	8.11265569771775	6.165799013020451	72.81402098178492
21	15.40320486036611	8.75520874504534	67.65778686210518	8.183799532483372
22	55.62318611453028	9.10302304834393	10.117105010558648	25.156685826567138
23	13.081431459126172	54.38663851027069	7.01500796133389	25.51692206926925
24	30.335505290616922	54.437455535103275	7.695956094090545	7.531083080189266
25	13.979198897835197	11.602091402888666	23.85915779250844	50.559551906767695
26	13.114180208462727	12.723453750861067	67.13154833828328	7.030817702392918
27	30.21354443101871	12.586812417422335	5.505177690196832	51.69446546136213
28	13.041907106478606	13.850462434925975	21.235870043928497	51.87176041466692
29	13.66865041274717	56.60790712906395	21.155692071414858	8.567750386774023
30	71.43857350964959	14.593520264700235	5.278194979277947	8.68971124637223
31	11.105213826747823	15.234944044809323	65.12258195656838	8.53726017187447
32	11.431572052894877	13.499260329971882	21.967635201517734	53.1015324156155
33	27.04060890776041	56.733896467612254	7.12349805763845	9.101996566988888
34	10.99680417377164	12.950436461779951	22.954614750488407	53.098144613959995
35	54.73445281356928	12.238998114123746	22.94445134552189	10.082097726785088
36	26.0759093424277	57.12398224791932	7.099703002721534	9.700405406931441
37	12.07638363465947	28.025024561562002	49.54772847898998	10.350863324788545
38	68.72381511637099	14.462525267354016	6.792542319289013	10.021117296985986
39	55.631021587427334	27.81900579004392	5.883243822593986	10.666728799934761
40	25.112644405045565	57.589240341942116	6.276467200433639	11.021648052578682
41	54.49956523212087	27.933553916863346	6.746242363330435	10.820638487685342
42	24.481384029902998	14.427517983580454	49.132158142581275	11.958939843935271
43	9.42825200727248	71.46793445733064	7.58077083780335	11.523042697593532
44	9.074791367881382	14.3360473388818	64.25417546554041	12.334985827696409
45	10.267297550619404	26.323218863279617	7.244249206689779	56.1652343794112
46	23.34308267365307	13.474416451164839	50.208349801813604	12.974151073368493
47	52.37993066299278	26.966901177825708	7.619165923232414	13.034002235949094
48	52.80792293880501	12.20737863200569	21.56900387338656	13.415694555802741
49	54.366311700337654	11.66984743599878	7.712895102368074	26.250945761295498
50	69.5538265219699	11.325420934355696	7.712895102368074	11.407857441306337
51	68.1354668955315	12.592458753514842	8.834257450340473	10.437816900613193
52	55.18841823540704	26.438404119566812	8.26623603943401	10.106941605592132
53	55.65480559664834	25.47062211331067	9.005906067552765	9.868666222488228
54	68.38277641638341	12.79120978397118	9.15045227152101	9.675561528124401
55	55.684166544329386	26.027350852032114	8.553069912933498	9.735412690705001
56	67.88815737467957	11.95329350784276	9.210303434101611	10.948245683376056
57	54.13594118776326	12.113649452870034	9.270154596682213	24.480254762684496
58	54.26919471954649	25.077637121272005	9.68685420030942	10.966313958872089
59	51.79497024380879	11.698079116461328	12.320305353855883	24.186645285873993
60	53.48096620103215	23.807211500457353	11.191038135353969	11.520784163156527
61	54.848508802637966	9.208044899664609	12.746039095231104	23.19740720246632
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	3.0
17	6.0
18	6.0
19	10.5
20	25.5
21	36.0
22	43.0
23	50.0
24	76.5
25	90.0
26	77.0
27	75.5
28	74.0
29	67.5
30	96.0
31	131.0
32	92.5
33	54.0
34	49.0
35	52.0
36	60.0
37	106.5
38	153.0
39	150.5
40	168.5
41	189.0
42	189.0
43	219.5
44	250.0
45	787.5
46	1325.0
47	7824.0
48	9849.0
49	5375.0
50	6015.0
51	6655.0
52	6631.5
53	5819.0
54	5030.0
55	4098.5
56	3167.0
57	2748.5
58	2726.5
59	3123.0
60	5625.5
61	18582.0
62	29036.0
63	14927.5
64	819.0
65	607.5
66	324.5
67	253.0
68	200.5
69	148.0
70	120.0
71	66.0
72	40.0
73	32.0
74	24.0
75	22.5
76	12.5
77	4.0
78	4.0
79	4.0
80	3.0
81	1.0
82	0.0
83	0.5
84	0.5
85	0.0
86	0.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.030490214899551678
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.001129267218501914
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.067089765451199
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	88553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.940194008108136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.54080045255267	26.044289860309643
2	12.738650827322868	8.137499576524792
3	2.9168434450572764	2.7949363657922373
4	0.8591429783623251	1.0976477363838606
5	0.3853768915287795	0.6154506340835432
6	0.2969876962240136	0.5691506781249647
7	0.1591005515485787	0.355719173828103
8	0.1414227124876255	0.3613655099206125
9	0.07778249186819403	0.223594909263379
>10	0.7424692405600339	4.823100290221675
>50	0.08131805968038468	1.8452226350321277
>100	0.03889124593409701	3.1224238591577924
>500	0.007071135624381275	2.15915892177566
>1k	0.007071135624381275	3.8835499644280826
>5k	0.0	0.0
>10k+	0.007071135624381275	43.96688988515353
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGGGG	27955	31.56866509322101	TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC	10979	12.398224791932515	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGGGT	2027	2.2890246519033797	TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGTGG	1412	1.5945253125247028	TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGGTG	997	1.1258794168464084	TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGNAGTATCTGGGGGGGGGGGGGGG	915	1.0332795049292514	TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGTGT	403	0.4550946890562714	TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATCGGAAGAGCACACGTCT	361	0.407665465879191	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGNGAGATAGATCGGAAGAGCACAC	353	0.3986313281311757	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTAGGGGGGGGGGGGGG	350	0.3952435264756699	TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGTGGG	331	0.3737874493241336	TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTAGAGAGATAGATCGGAAGAGCACACG	251	0.28344607184398046	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGGTT	214	0.24166318475940962	TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAAGAGATAGATCGGAAGAGCACACG	162	0.18294128939731008	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGTTG	135	0.1524510744977584	TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGGGAGATAGATCGGAAGAGCACAC	104	0.11744379072419907	No Hit
CTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACGTC	101	0.11405598906869333	No Hit
AGATCGGAAGAGCACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATC	96	0.10840965297618375	TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCT	95	0.10728038575768184	TruSeq Adapter, Index 9 (97% over 36bp)
CTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACG	93	0.10502185132067801	No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGGG	91	0.1027633168836742	TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACGTC	89	0.10050478244667035	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	44.18257992388739	0.0	0.0	0.0	0.0
2	44.411821169243275	0.0	0.0	0.0	0.0
3	44.56540151095954	0.0	0.0	0.0	0.0
4	44.734791593734826	0.0	0.0	0.0	0.0
5	45.12325951689948	0.0	0.0	0.0	0.0
6	45.63707610131785	0.0	0.0	0.0	0.0
7	46.408365611554665	0.0	0.0	0.0	0.0
8	53.32173952322338	0.0	0.0	0.0	0.0
9	53.97784377717299	0.0	0.0	0.0	0.0
10	54.50069449933938	0.0	0.0	0.0	0.0
11	54.95127211952164	0.0	0.0	0.0	0.0
12	55.697717750951405	0.0	0.0	0.0	0.0
13	56.53676329429833	0.0	0.0	0.0	0.0
14	57.48986482671395	0.0	0.0	0.0	0.0
15	58.750127042562085	0.0	0.0	0.0	0.0
16	60.056689214368795	0.0	0.0	0.0	0.0
17	61.03350535837295	0.0	0.0	0.0	0.0
18	61.5913633643129	0.0	0.0	0.0	0.0
19	62.48687226858492	0.0	0.0	0.0	0.0
20	62.97358643975924	0.0	0.0	0.0	0.0
21	63.45691280927806	0.0	0.0	0.0	0.0
22	63.901844093367814	0.0	0.0	0.0	0.0
23	64.45179722877825	0.0	0.0	0.0	0.0
24	64.92044312445654	0.0	0.0	0.0	0.0
25	65.40376949397536	0.0	0.0	0.0	0.0
26	65.93678362110826	0.0	0.0	0.0	0.0
27	66.48334895486319	0.0	0.0	0.0	0.0
28	67.09315325285422	0.0	0.0	0.0	0.0
29	67.69505268031574	0.0	0.0	0.0	0.0
30	68.440369044527	0.0	0.0	0.0	0.0
31	69.27150971734441	0.0	0.0	0.0	0.0
32	70.0868406491028	0.0	0.0	0.0	0.0
33	71.02074463880388	0.0	0.0	0.0	0.0
34	71.8609194493693	0.0	0.0	0.0	0.0
35	72.60623581358057	0.0	0.0	0.0	0.0
36	73.25443519700066	0.0	0.0	0.0	0.0
37	73.85520535724369	0.0	0.0	0.0	0.0
38	74.25157815093786	0.0	0.0	0.0	0.0
39	74.62084853138798	0.0	0.0	0.0	0.0
40	74.95962869693855	0.0	0.0	0.0	0.0
41	75.70720359558682	0.0	0.0	0.0	0.0
42	76.2198909127867	0.0	0.0	0.0	0.0
43	76.61287590482536	0.0	0.0	0.0	0.0
44	77.99622824749021	0.0	0.0	0.0	0.0
45	92.05334658340203	0.0	0.0	0.0	0.0
46	92.5084412724583	0.0	0.0	0.0	0.0
47	92.65072894198954	0.0	0.0	0.0	0.0
48	92.80882635257981	0.0	0.0	0.0	0.0
49	92.94998475489255	0.0	0.0	0.0	0.0
50	93.07533341614626	0.0	0.0	0.0	0.0
>>END_MODULE