FastQCFastQC Report
Sat 10 Aug 2024
AM-AL-EC06_S56_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-EC06_S56_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88553
Sequences flagged as poor quality0
Sequence length61
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGGGG2795531.56866509322101TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC1097912.398224791932515No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGGGT20272.2890246519033797TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGTGG14121.5945253125247028TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGGTG9971.1258794168464084TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGNAGTATCTGGGGGGGGGGGGGGG9151.0332795049292514TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGTGT4030.4550946890562714TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATCGGAAGAGCACACGTCT3610.407665465879191Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGNGAGATAGATCGGAAGAGCACAC3530.3986313281311757No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTAGGGGGGGGGGGGGG3500.3952435264756699TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGTGGG3310.3737874493241336TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTAGAGAGATAGATCGGAAGAGCACACG2510.28344607184398046No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGGTT2140.24166318475940962TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAAGAGATAGATCGGAAGAGCACACG1620.18294128939731008No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGTTG1350.1524510744977584TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGGGAGATAGATCGGAAGAGCACAC1040.11744379072419907No Hit
CTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACGTC1010.11405598906869333No Hit
AGATCGGAAGAGCACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATC960.10840965297618375TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCT950.10728038575768184TruSeq Adapter, Index 9 (97% over 36bp)
CTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACG930.10502185132067801No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGGAGTATCTGGGGGGGGGGGGGG910.1027633168836742TruSeq Adapter, Index 9 (97% over 36bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACGTC890.10050478244667035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)