##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-EC06.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8082 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.890373669883694 40.0 40.0 40.0 40.0 40.0 2 38.78074733976739 40.0 40.0 40.0 40.0 40.0 3 38.91512001979708 40.0 40.0 40.0 40.0 40.0 4 38.76713684731502 40.0 40.0 40.0 40.0 40.0 5 38.95421925266023 40.0 40.0 40.0 40.0 40.0 6 39.11210096510764 40.0 40.0 40.0 40.0 40.0 7 39.16307844592922 40.0 40.0 40.0 40.0 40.0 8 39.00222717149221 40.0 40.0 40.0 40.0 40.0 9 39.29967829745112 40.0 40.0 40.0 40.0 40.0 10-11 39.25018559762435 40.0 40.0 40.0 40.0 40.0 12-13 39.235337787676315 40.0 40.0 40.0 40.0 40.0 14-15 39.3192279138827 40.0 40.0 40.0 40.0 40.0 16-17 39.34867607027964 40.0 40.0 40.0 40.0 40.0 18-19 39.323682256867116 40.0 40.0 40.0 40.0 40.0 20-21 39.16530561742143 40.0 40.0 40.0 40.0 40.0 22-23 39.15367483296214 40.0 40.0 40.0 40.0 40.0 24-25 39.16975996040584 40.0 40.0 40.0 40.0 40.0 26-27 38.81069042316258 40.0 40.0 40.0 40.0 40.0 28-29 38.60950259836674 40.0 40.0 40.0 40.0 40.0 30-31 38.83257740584749 40.0 40.0 40.0 40.0 40.0 32-33 38.96500111024191 40.0 40.0 40.0 40.0 40.0 34-35 39.01634450758067 40.0 40.0 40.0 40.0 40.0 36-37 39.01314504496774 40.0 40.0 40.0 40.0 40.0 38-39 38.866927684256844 40.0 40.0 40.0 40.0 40.0 40-41 38.96102407509038 40.0 40.0 40.0 40.0 40.0 42-43 38.82641992135133 40.0 40.0 40.0 40.0 40.0 44-45 38.59335352472674 40.0 40.0 40.0 40.0 40.0 46-47 38.62233585263929 40.0 40.0 40.0 40.0 40.0 48-49 38.537095300923575 40.0 40.0 40.0 40.0 40.0 50-51 38.48122640621291 40.0 40.0 40.0 40.0 40.0 52-53 38.248321170140144 40.0 40.0 40.0 40.0 40.0 54-55 38.198471774866746 40.0 40.0 40.0 32.0 40.0 56-57 38.115472864546 40.0 40.0 40.0 40.0 40.0 58-59 37.974264003721814 40.0 40.0 40.0 24.0 40.0 60-61 37.92738774370849 40.0 40.0 40.0 24.0 40.0 62-63 38.966004606103354 40.0 40.0 40.0 40.0 40.0 64-65 38.958253050738605 40.0 40.0 40.0 40.0 40.0 66-67 39.17509157509158 40.0 40.0 40.0 40.0 40.0 68-69 38.8486013986014 40.0 40.0 40.0 40.0 40.0 70-71 38.959829741952646 40.0 40.0 40.0 40.0 40.0 72-73 39.06658267106029 40.0 40.0 40.0 40.0 40.0 74-75 39.04292973035395 40.0 40.0 40.0 40.0 40.0 76-77 38.61549578482537 40.0 40.0 40.0 40.0 40.0 78-79 38.79706729510343 40.0 40.0 40.0 40.0 40.0 80-81 38.281179138321995 40.0 40.0 40.0 40.0 40.0 82-83 38.843205574912886 40.0 40.0 40.0 40.0 40.0 84-85 38.43171521035599 40.0 40.0 40.0 24.0 40.0 86-87 38.51151315789474 NaN NaN NaN NaN NaN 88-89 38.87276245766812 NaN NaN NaN NaN NaN 90-91 40.0 NaN NaN NaN NaN NaN >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1413970334288308 1101 2 0.9014741067280028 1101 3 0.75876753907135 1101 4 0.22545330373706918 1101 5 0.7125578380091682 1101 6 1.0561309162666106 1101 7 -0.8747911224431135 1101 8 1.1292501890942361 1101 9 0.7544506824844177 1101 10-11 0.6899495420111421 1101 12-13 0.7408701823240236 1101 14-15 0.585260673438988 1101 16-17 0.6610945688650318 1101 18-19 0.5923542377149147 1101 20-21 0.779657337532889 1101 22-23 0.0086606924596353 1101 24-25 -0.9439567424435822 1101 26-27 -1.7230622755172433 1101 28-29 -0.6991058404473947 1101 30-31 0.1651003073610653 1101 32-33 -0.27625621713228554 1101 34-35 0.3727552712968816 1101 36-37 0.15579031311181524 1101 38-39 0.7946404290154305 1101 40-41 -1.0991992382617397 1101 42-43 0.7820937742812717 1101 44-45 1.4896735209235246 1101 46-47 -3.168898809523803 1101 48-49 -2.883010912698417 1101 50-51 1.3966765873015845 1101 52-53 -0.5212797619047649 1101 54-55 -0.24166666666666003 1101 56-57 0.5656250000000043 1101 58-59 2.589583333333337 1101 60-61 5.287500000000001 1101 62-63 4.768749999999997 1101 64-65 9.975000000000001 1101 66-67 10.774999999999999 1101 68-69 13.933333333333334 1101 70-71 15.73333333333333 1101 72-73 15.625 1101 74-75 19.0625 1101 76-77 -17.25 1101 78-79 -16.0 1101 80-81 -15.4375 1101 82-83 -13.1875 1101 84-85 -10.75 1101 86-87 -8.75 1101 88-89 -5.3125 1101 90-91 -2.5 1102 1 0.941397033428828 1102 2 0.9014741067280028 1102 3 0.75876753907135 1102 4 1.0254533037370663 1102 5 0.7125578380091682 1102 6 0.21402565310871324 1102 7 0.7252088775568879 1102 8 1.1292501890942361 1102 9 0.7544506824844177 1102 10-11 0.6899495420111421 1102 12-13 0.7408701823240236 1102 14-15 -0.25684458971890933 1102 16-17 0.6610945688650318 1102 18-19 0.5923542377149147 1102 20-21 0.779657337532889 1102 22-23 0.38760806088068733 1102 24-25 0.856043257556415 1102 26-27 1.1769377244827552 1102 28-29 -0.07805320886844669 1102 30-31 0.42299504420316936 1102 32-33 0.41263267175660445 1102 34-35 -1.211860113318508 1102 36-37 -0.11693695961545814 1102 38-39 0.7946404290154305 1102 40-41 0.6508007617382603 1102 42-43 0.7820937742812717 1102 44-45 1.4896735209235246 1102 46-47 -1.1688988095238102 1102 48-49 1.5169890873015817 1102 50-51 1.3966765873015845 1102 52-53 1.4787202380952351 1102 54-55 3.25833333333334 1102 56-57 4.065625000000004 1102 58-59 4.589583333333337 1102 60-61 5.287500000000001 1102 62-63 7.435416666666669 1102 64-65 1.9750000000000014 1102 66-67 10.774999999999999 1102 68-69 13.933333333333334 1102 70-71 15.73333333333333 1102 72-73 15.625 1102 74-75 19.0625 1102 76-77 22.75 1102 78-79 24.0 1102 80-81 24.5625 1102 82-83 26.8125 1102 84-85 29.25 1102 86-87 31.25 1102 88-89 34.6875 1102 90-91 -2.5 1103 1 0.2457448595157814 1103 2 -0.48983024109808326 1103 3 0.0631153651583034 1103 4 -0.3658510440890197 1103 5 0.016905664096121598 1103 6 -0.16126038808121734 1103 7 0.7252088775568879 1103 8 -0.2620541587318499 1103 9 0.7544506824844177 1103 10-11 -0.005702631901904454 1103 12-13 -0.30260807854553917 1103 14-15 0.585260673438988 1103 16-17 0.6610945688650318 1103 18-19 -0.10329793619813188 1103 20-21 0.43183125057636573 1103 22-23 -0.495687133627321 1103 24-25 0.5082171705998917 1103 26-27 0.829111637526232 1103 28-29 0.5443724204221709 1103 30-31 0.30857856823062946 1103 32-33 0.024913373510990766 1103 34-35 -1.027244728703124 1103 36-37 -0.8947147373932367 1103 38-39 -0.7609151265401266 1103 40-41 -0.9047547938172968 1103 42-43 -0.5512395590520569 1103 44-45 -0.5103264790764754 1103 46-47 -3.168898809523803 1103 48-49 -3.1496775793650826 1103 50-51 -1.269990079365087 1103 52-53 -3.187946428571429 1103 54-55 3.25833333333334 1103 56-57 -0.60104166666666 1103 58-59 -0.7437499999999986 1103 60-61 2.62083333333333 1103 62-63 7.435416666666669 1103 64-65 9.975000000000001 1103 66-67 10.774999999999999 1103 68-69 13.933333333333334 1103 70-71 15.73333333333333 1103 72-73 15.625 1103 74-75 -20.9375 1103 76-77 -17.25 1103 78-79 -16.0 1103 80-81 -15.4375 1103 82-83 -13.1875 1103 84-85 -10.75 1103 86-87 -8.75 1103 88-89 -5.3125 1103 90-91 -2.5 1104 1 -0.275994270919 1104 2 -0.3159171976198252 1104 3 0.75876753907135 1104 4 -0.3658510440890197 1104 5 0.7125578380091682 1104 6 -0.8569125619942568 1104 7 0.7252088775568879 1104 8 1.1292501890942361 1104 9 0.7544506824844177 1104 10-11 -0.005702631901904454 1104 12-13 0.3930440953675003 1104 14-15 0.23743458648246474 1104 16-17 -0.7302097789610613 1104 18-19 -0.36416750141552257 1104 20-21 0.43183125057636573 1104 22-23 -1.4522088727577582 1104 24-25 0.856043257556415 1104 26-27 0.4812855505697087 1104 28-29 0.8052419856395616 1104 30-31 0.9172742204045434 1104 32-33 0.10386074193204564 1104 34-35 0.11313988668149477 1104 36-37 -0.7169369596154596 1104 38-39 0.12797376234875912 1104 40-41 0.6508007617382603 1104 42-43 0.7820937742812717 1104 44-45 -2.176993145743147 1104 46-47 -2.9022321428571445 1104 48-49 -1.9830109126984183 1104 50-51 1.3966765873015845 1104 52-53 1.4787202380952351 1104 54-55 -2.0749999999999957 1104 56-57 1.3989583333333329 1104 58-59 -2.0770833333333343 1104 60-61 -5.712499999999999 1104 62-63 0.43541666666666856 1104 64-65 1.9750000000000014 1104 66-67 10.774999999999999 1104 68-69 13.933333333333334 1104 70-71 -0.26666666666666927 1104 72-73 -0.375 1104 74-75 19.0625 1104 76-77 -17.25 1104 78-79 -16.0 1104 80-81 -15.4375 1104 82-83 -13.1875 1104 84-85 -10.75 1104 86-87 -8.75 1104 88-89 -5.3125 1104 90-91 -2.5 1201 1 0.941397033428828 1201 2 -0.7827364195877848 1201 3 0.75876753907135 1201 4 1.0254533037370663 1201 5 0.7125578380091682 1201 6 1.0561309162666106 1201 7 -0.11689638560100946 1201 8 0.2871449259363388 1201 9 -0.08765458067347964 1201 10-11 0.6899495420111421 1201 12-13 -0.10123508083386668 1201 14-15 -0.5726340634031217 1201 16-17 0.2400419372860867 1201 18-19 0.17130160613596956 1201 20-21 -0.37823739930922073 1201 22-23 0.8086606924596325 1201 24-25 -0.8281672687593726 1201 26-27 -1.3493780649909297 1201 28-29 -1.0254216299210768 1201 30-31 -0.7348996926389333 1201 32-33 0.07096600508993589 1201 34-35 0.26086715940876815 1201 36-37 0.8830630403845419 1201 38-39 0.7946404290154305 1201 40-41 0.6508007617382603 1201 42-43 -2.551239559052057 1201 44-45 1.4896735209235246 1201 46-47 -1.1688988095238102 1201 48-49 -0.08301091269841976 1201 50-51 -2.1033234126984155 1201 52-53 -2.021279761904765 1201 54-55 3.25833333333334 1201 56-57 2.0656250000000043 1201 58-59 4.589583333333337 1201 60-61 0.6208333333333371 1201 62-63 2.102083333333333 1201 64-65 9.975000000000001 1201 66-67 6.774999999999999 1201 68-69 -4.066666666666666 1201 70-71 -12.26666666666667 1201 72-73 15.625 1201 74-75 11.0625 1201 76-77 22.75 1201 78-79 16.0 1201 80-81 2.5625 1201 82-83 12.8125 1201 84-85 21.25 1201 86-87 31.25 1201 88-89 26.6875 1201 90-91 37.5 1202 1 -0.8363807443489506 1202 2 -1.1725999673460734 1202 3 -0.42641764611383337 1202 4 -0.159731881448117 1202 5 -2.5467014212500914 1202 6 -2.4994246392889465 1202 7 0.7252088775568879 1202 8 -0.5003794405353901 1202 9 -0.43073450270076563 1202 10-11 -0.19893934687774362 1202 12-13 -0.4443150028611669 1202 14-15 -1.1184430302647144 1202 16-17 -0.15372024594977773 1202 18-19 -0.29653465117397104 1202 20-21 -2.0351574772819205 1202 22-23 -0.3024504186514818 1202 24-25 0.26345066496381975 1202 26-27 -1.41565486810984 1202 28-29 0.09348675214520341 1202 30-31 -2.068233025972262 1202 32-33 -0.7510036918797596 1202 34-35 -0.011860113318505228 1202 36-37 -3.0290248717033705 1202 38-39 -3.051513417138416 1202 40-41 -5.34919923826174 1202 42-43 -3.8179062257187297 1202 44-45 -2.1992153679653654 1202 46-47 -3.002232142857139 1202 48-49 -3.375868055555557 1202 50-51 -1.3461805555555628 1202 52-53 1.4787202380952351 1202 54-55 3.25833333333334 1202 56-57 4.065625000000004 1202 58-59 4.589583333333337 1202 60-61 5.287500000000001 1202 62-63 7.435416666666669 1202 64-65 9.975000000000001 1202 66-67 -29.225 1202 68-69 -26.066666666666666 1202 70-71 -24.26666666666667 1202 72-73 -24.375 1202 74-75 -20.9375 1202 76-77 -17.25 1202 78-79 -16.0 1202 80-81 -15.4375 1202 82-83 -13.1875 1202 84-85 -10.75 1202 86-87 -8.75 1202 88-89 -5.3125 1202 90-91 -2.5 1203 1 -1.558602966571172 1203 2 0.23480744006133847 1203 3 0.09210087240468567 1203 4 -0.30788002959626937 1203 5 0.04589117134250387 1203 6 -0.2772024170667251 1203 7 -0.6081244557764478 1203 8 -0.8707498109057639 1203 9 0.7544506824844177 1203 10-11 -0.5600504579888579 1203 12-13 -0.2591298176759764 1203 14-15 0.2519273401056594 1203 16-17 -0.005572097801639586 1203 18-19 0.5923542377149147 1203 20-21 0.4463240041995604 1203 22-23 0.47532735912630386 1203 24-25 0.18937659088975067 1203 26-27 -0.48972894218391616 1203 28-29 0.2508941595526082 1203 30-31 0.5984336406944024 1203 32-33 0.9459660050899359 1203 34-35 0.04696341609326282 1203 36-37 0.008063040384541864 1203 38-39 -0.20535957098456947 1203 40-41 0.6508007617382603 1203 42-43 0.7820937742812717 1203 44-45 1.4896735209235246 1203 46-47 0.4977678571428612 1203 48-49 -1.4830109126984183 1203 50-51 -0.6033234126984155 1203 52-53 1.4787202380952351 1203 54-55 3.25833333333334 1203 56-57 -0.60104166666666 1203 58-59 4.589583333333337 1203 60-61 5.287500000000001 1203 62-63 7.435416666666669 1203 64-65 9.975000000000001 1203 66-67 10.774999999999999 1203 68-69 13.933333333333334 1203 70-71 15.73333333333333 1203 72-73 15.625 1203 74-75 19.0625 1203 76-77 22.75 1203 78-79 24.0 1203 80-81 24.5625 1203 82-83 -13.1875 1203 84-85 -10.75 1203 86-87 -8.75 1203 88-89 -5.3125 1203 90-91 -2.5 1204 1 0.941397033428828 1204 2 0.9014741067280028 1204 3 0.75876753907135 1204 4 0.26354854183230714 1204 5 0.7125578380091682 1204 6 -1.229583369447674 1204 7 -0.7986006462526376 1204 8 -0.20408314423909957 1204 9 0.7544506824844177 1204 10-11 -0.6433837913221865 1204 12-13 0.7408701823240236 1204 14-15 0.585260673438988 1204 16-17 -0.3865244787540192 1204 18-19 0.2114018567625351 1204 20-21 0.779657337532889 1204 22-23 0.42770831150725286 1204 24-25 -0.5725281710150156 1204 26-27 0.7959853435303756 1204 28-29 0.3580370166954694 1204 30-31 0.12224316450392791 1204 32-33 0.4459660050899359 1204 34-35 -0.6499553514137446 1204 36-37 -0.34770619038468453 1204 38-39 0.7946404290154305 1204 40-41 0.6508007617382603 1204 42-43 0.7820937742812717 1204 44-45 0.6007846320346388 1204 46-47 1.4977678571428612 1204 48-49 -0.8163442460317469 1204 50-51 1.3966765873015845 1204 52-53 -0.1212797619047663 1204 54-55 3.25833333333334 1204 56-57 4.065625000000004 1204 58-59 4.589583333333337 1204 60-61 5.287500000000001 1204 62-63 7.435416666666669 1204 64-65 9.975000000000001 1204 66-67 10.774999999999999 1204 68-69 13.933333333333334 1204 70-71 15.73333333333333 1204 72-73 15.625 1204 74-75 19.0625 1204 76-77 18.75 1204 78-79 24.0 1204 80-81 20.5625 1204 82-83 26.8125 1204 84-85 29.25 1204 86-87 -8.75 1204 88-89 -5.3125 1204 90-91 -2.5 1301 1 0.1413970334288308 1301 2 0.9014741067280028 1301 3 -0.041232460928647185 1301 4 1.0254533037370663 1301 5 -0.08744216199082899 1301 6 0.25613091626661344 1301 7 0.7252088775568879 1301 8 -0.4707498109057653 1301 9 -0.045549317515579446 1301 10-11 -0.11005045798885504 1301 12-13 -0.35912981767597074 1301 14-15 0.585260673438988 1301 16-17 -0.538905431134971 1301 18-19 -0.20764576228508247 1301 20-21 -0.4203426624671138 1301 22-23 0.4086606924596339 1301 24-25 0.056043257556417814 1301 26-27 1.1769377244827552 1301 28-29 0.700894159552611 1301 30-31 0.8651003073610681 1301 32-33 0.4459660050899359 1301 34-35 -0.24262934408773162 1301 36-37 -0.7169369596154596 1301 38-39 -0.5386929043178981 1301 40-41 0.6508007617382603 1301 42-43 0.7820937742812717 1301 44-45 1.4896735209235246 1301 46-47 1.4977678571428612 1301 48-49 1.5169890873015817 1301 50-51 1.3966765873015845 1301 52-53 1.4787202380952351 1301 54-55 3.25833333333334 1301 56-57 4.065625000000004 1301 58-59 4.589583333333337 1301 60-61 5.287500000000001 1301 62-63 7.435416666666669 1301 64-65 9.975000000000001 1301 66-67 10.774999999999999 1301 68-69 13.933333333333334 1301 70-71 15.73333333333333 1301 72-73 15.625 1301 74-75 19.0625 1301 76-77 22.75 1301 78-79 24.0 1301 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2404 2 -0.012811607557708271 2404 3 0.30162468192849445 2404 4 0.5683104465942108 2404 5 0.25541498086631265 2404 6 0.5989880591237551 2404 7 0.7252088775568879 2404 8 1.1292501890942361 2404 9 -0.15983503180129333 2404 10-11 0.6899495420111421 2404 12-13 0.7408701823240236 2404 14-15 0.585260673438988 2404 16-17 0.43252314029360406 2404 18-19 -0.32193147657079635 2404 20-21 0.3225144803900335 2404 22-23 -0.33419645039750634 2404 24-25 0.6274718289849872 2404 26-27 0.49122343876847196 2404 28-29 1.2723227309811804 2404 30-31 1.2651003073610667 2404 32-33 0.17983697283187183 2404 34-35 0.6403137997249715 2404 36-37 -0.35904222277335407 2404 38-39 0.7946404290154305 2404 40-41 0.1508007617382603 2404 42-43 0.2820937742812717 2404 44-45 0.6896735209235274 2404 46-47 0.6088789682539755 2404 48-49 1.5169890873015817 2404 50-51 1.3966765873015845 2404 52-53 -0.1212797619047663 2404 54-55 3.25833333333334 2404 56-57 2.0656250000000043 2404 58-59 2.589583333333337 2404 60-61 5.287500000000001 2404 62-63 7.435416666666669 2404 64-65 9.975000000000001 2404 66-67 10.774999999999999 2404 68-69 13.933333333333334 2404 70-71 7.733333333333331 2404 72-73 15.625 2404 74-75 19.0625 2404 76-77 22.75 2404 78-79 24.0 2404 80-81 24.5625 2404 82-83 26.8125 2404 84-85 -10.75 2404 86-87 -8.75 2404 88-89 -5.3125 2404 90-91 -2.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 31 14.0 32 59.0 33 118.0 34 155.0 35 193.0 36 327.0 37 489.0 38 846.0 39 2426.0 40 3455.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.223459539717894 24.536005939123978 36.16679039841623 22.073744122741896 2 27.249102833807697 20.876129191931692 26.29625046405148 25.57851751020913 3 27.703538728037614 19.53724325661965 27.85201682751794 24.907201187824796 4 26.651818856718634 19.561989606533036 24.164810690423163 29.621380846325167 5 27.270477604553328 20.997277901509527 27.703538728037614 24.02870576589953 6 28.594407324919572 23.447166542934916 23.76886909180896 24.189557040336553 7 27.047760455332835 25.105171987131897 25.538233110616183 22.30883444691908 8 18.720613709477853 46.38703291264539 14.117792625587725 20.774560752289037 9 45.756000989853995 18.46077703538728 17.73075971294234 18.05246226181638 10-11 17.718386537985648 28.76763177431329 33.2900272209849 20.223954466716158 12-13 36.42044048502846 14.068299925760948 15.800544419698095 33.7107151695125 14-15 33.65503588220737 31.848552338530066 13.666171739668398 20.830240039594162 16-17 31.836179163573377 31.53922296461272 13.288789903489235 23.335807968324673 18-19 32.98688443454591 23.113090819104183 16.567681267013114 27.332343479336796 20-21 33.06731007176442 11.643157634248947 22.302647859440732 32.98688443454591 22-23 29.318238059886166 13.33828260331601 27.963375402128186 29.380103934669638 24-25 34.7253155159614 15.874783469438258 29.63375402128186 19.766146993318486 26-27 34.552091066567684 24.239049740163328 23.50284583024004 17.70601336302895 28-29 38.63523880227667 26.243504083147734 17.600841375897055 17.520415738678544 30-31 31.790619972775648 36.16507857938374 14.86202202697686 17.182279420863754 32-33 29.92203466807963 32.336689122700776 18.492165619559458 19.24911058966013 34-35 27.40339547963754 24.966149359441726 24.174565149463596 23.45589001145714 36-37 27.815866797257588 23.88414719462712 20.791940674408842 27.50804533370645 38-39 27.724920714404938 23.935903855783675 20.44733767317643 27.891837756634953 40-41 27.582920320243996 23.770491803278688 20.815859702630576 27.83072817384674 42-43 23.895582329317268 25.301204819277107 24.67648371262829 26.126729138777332 44-45 22.2729715206878 21.97743148844707 26.92101020956475 28.828586781300373 46-47 22.318840579710145 23.18840579710145 25.539452495974235 28.95330112721417 48-49 22.238372093023255 24.055232558139537 24.927325581395348 28.77906976744186 50-51 21.164021164021165 23.402523402523403 26.21082621082621 29.22262922262922 52-53 20.96483318304779 24.436429215509467 25.924256086564473 28.67448151487827 54-55 22.087745839636913 22.844175491679273 26.27332324760464 28.79475542107917 56-57 22.108843537414966 23.015873015873016 25.45351473922903 29.421768707482993 58-59 23.49936143039591 22.094508301404854 26.69220945083014 27.713920817369093 60-61 22.494744218640506 22.214435879467416 26.20882971268395 29.081990189208128 62-63 23.42857142857143 20.816326530612244 26.448979591836736 29.306122448979593 64-65 24.06367041198502 20.880149812734082 28.651685393258425 26.40449438202247 66-67 24.093816631130064 21.85501066098081 26.226012793176974 27.825159914712156 68-69 27.020506634499398 21.23039806996381 24.96984318455971 26.77925211097708 70-71 21.603260869565215 19.021739130434785 28.532608695652172 30.842391304347828 72-73 25.194401244167963 20.839813374805598 28.615863141524105 25.34992223950233 74-75 25.996204933586338 23.14990512333966 25.23719165085389 25.616698292220114 76-77 22.595078299776286 23.2662192393736 26.62192393736018 27.516778523489933 78-79 24.55242966751918 21.994884910485936 26.08695652173913 27.365728900255753 80-81 22.32704402515723 21.069182389937108 28.30188679245283 28.30188679245283 82-83 27.34375 23.828125 19.53125 29.296875 84-85 20.207253886010363 22.797927461139896 29.015544041450774 27.979274611398964 86-87 17.857142857142858 24.285714285714285 25.71428571428571 32.142857142857146 88-89 20.652173913043477 10.869565217391305 34.78260869565217 33.69565217391305 90-91 6.896551724137931 31.03448275862069 31.03448275862069 31.03448275862069 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 1.0 14 1.0 15 3.0 16 6.5 17 8.0 18 6.5 19 5.5 20 8.0 21 8.5 22 6.0 23 9.0 24 15.5 25 19.5 26 19.0 27 17.0 28 18.0 29 19.0 30 19.5 31 22.0 32 23.5 33 25.0 34 27.5 35 31.5 36 38.5 37 53.5 38 69.0 39 88.5 40 154.0 41 269.5 42 413.0 43 558.0 44 741.0 45 889.5 46 975.0 47 1091.5 48 1165.5 49 1191.0 50 1190.0 51 1109.0 52 990.0 53 858.5 54 743.5 55 679.5 56 593.5 57 524.0 58 490.0 59 458.0 60 430.0 61 402.5 62 383.5 63 366.5 64 360.0 65 368.5 66 373.5 67 364.0 68 347.5 69 332.0 70 313.5 71 286.5 72 250.0 73 200.5 74 158.0 75 136.5 76 126.5 77 107.0 78 84.0 79 67.5 80 53.0 81 44.5 82 36.5 83 25.5 84 16.5 85 12.5 86 9.5 87 5.0 88 2.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.012373174956693888 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.21652231845436376 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 990.0 32-33 1889.0 34-35 1420.0 36-37 682.0 38-39 384.0 40-41 379.0 42-43 372.0 44-45 364.0 46-47 186.0 48-49 158.0 50-51 119.0 52-53 120.0 54-55 108.0 56-57 106.0 58-59 72.0 60-61 91.0 62-63 93.0 64-65 66.0 66-67 54.0 68-69 51.0 70-71 43.0 72-73 59.0 74-75 41.0 76-77 34.0 78-79 30.0 80-81 38.0 82-83 30.0 84-85 27.0 86-87 23.0 88-89 36.0 90-91 17.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.77728285077951 #Duplication Level Percentage of deduplicated Percentage of total 1 99.81398809523809 99.5916852264291 2 0.1488095238095238 0.2969561989606533 3 0.03720238095238095 0.11135857461024498 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.07423904974016332 0.0 0.0 0.0 0.0 2 0.07423904974016332 0.0 0.0 0.0 0.0 3 0.07423904974016332 0.0 0.0 0.0 0.0 4 0.0989853996535511 0.0 0.0 0.0 0.0 5 0.2845830240039594 0.0 0.0 0.0 0.0 6 0.7423904974016332 0.0 0.0 0.0 0.0 7 1.880722593417471 0.0 0.0 0.0 0.0 8 15.961395694135115 0.0 0.0 0.0 0.0 9 16.815144766146993 0.0 0.0 0.0 0.0 10-11 16.864637465973768 0.0 0.0 0.0 0.0 12-13 16.87701064093046 0.0 0.0 0.0 0.0 14-15 16.88319722840881 0.0 0.0 0.0 0.0 16-17 16.90175699084385 0.0 0.0 0.0 0.0 18-19 16.90175699084385 0.0 0.0 0.0 0.0 20-21 16.90175699084385 0.0 0.0 0.0 0.0 22-23 16.90175699084385 0.0 0.0 0.0 0.0 24-25 16.914130165800543 0.0 0.0 0.0 0.0 26-27 16.94506310319228 0.0 0.0 0.0 0.0 28-29 16.988369215540708 0.0 0.0 0.0 0.0 30-31 17.019302152932443 0.0 0.0 0.0 0.0 32-33 17.050235090324176 0.0 0.0 0.0 0.0 34-35 17.050235090324176 0.0 0.0 0.0 0.0 36-37 17.062608265280872 0.0 0.0 0.0 0.0 38-39 17.08116802771591 0.0 0.0 0.0 0.0 40-41 17.099727790150954 0.0 0.0 0.0 0.0 42-43 17.112100965107647 0.0 0.0 0.0 0.0 44-45 17.130660727542686 0.0 0.0 0.0 0.0 46-47 17.136847315021033 0.0 0.0 0.0 0.0 48-49 17.136847315021033 0.0 0.0 0.0 0.0 50-51 17.136847315021033 0.0 0.0 0.0 0.0 52-53 17.136847315021033 0.0 0.0 0.0 0.0 54-55 17.136847315021033 0.0 0.0 0.0 0.0 56-57 17.136847315021033 0.0 0.0 0.0 0.0 58-59 17.136847315021033 0.0 0.0 0.0 0.0 60-61 17.136847315021033 0.0 0.0 0.0 0.0 62-63 17.136847315021033 0.0 0.0 0.0 0.0 64-65 17.136847315021033 0.0 0.0 0.0 0.0 66-67 17.136847315021033 0.0 0.0 0.0 0.0 68-69 17.136847315021033 0.0 0.0 0.0 0.0 70-71 17.136847315021033 0.0 0.0 0.0 0.0 72-73 17.136847315021033 0.0 0.0 0.0 0.0 74-75 17.136847315021033 0.0 0.0 0.0 0.0 76-77 17.136847315021033 0.0 0.0 0.0 0.0 78-79 17.136847315021033 0.0 0.0 0.0 0.0 80 17.136847315021033 0.0 0.0 0.0 0.0 >>END_MODULE