##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-EC05_S55_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162010
Sequences flagged as poor quality	0
Sequence length	61
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.55322510956114	40.0	40.0	40.0	40.0	40.0
2	39.471810382075184	40.0	40.0	40.0	40.0	40.0
3	39.393617677921114	40.0	40.0	40.0	40.0	40.0
4	39.482649219184	40.0	40.0	40.0	40.0	40.0
5	38.34010246281094	40.0	40.0	40.0	40.0	40.0
6	39.41630763533115	40.0	40.0	40.0	40.0	40.0
7	38.167940250601816	40.0	40.0	40.0	40.0	40.0
8	39.31216591568422	40.0	40.0	40.0	40.0	40.0
9	38.75787914326276	40.0	40.0	40.0	40.0	40.0
10	39.33088080982655	40.0	40.0	40.0	40.0	40.0
11	39.29313005370039	40.0	40.0	40.0	40.0	40.0
12	39.313227578544534	40.0	40.0	40.0	40.0	40.0
13	38.8096537250787	40.0	40.0	40.0	40.0	40.0
14	39.34048515523733	40.0	40.0	40.0	40.0	40.0
15	39.428059996296525	40.0	40.0	40.0	40.0	40.0
16	39.281575211406704	40.0	40.0	40.0	40.0	40.0
17	39.35055860749336	40.0	40.0	40.0	40.0	40.0
18	39.36206407011913	40.0	40.0	40.0	40.0	40.0
19	39.3322387506944	40.0	40.0	40.0	40.0	40.0
20	34.03493611505463	40.0	24.0	40.0	24.0	40.0
21	35.0661810999321	40.0	24.0	40.0	24.0	40.0
22	36.496117523609655	40.0	40.0	40.0	24.0	40.0
23	38.526263810875875	40.0	40.0	40.0	40.0	40.0
24	38.91441269057466	40.0	40.0	40.0	40.0	40.0
25	34.764002222085054	40.0	24.0	40.0	24.0	40.0
26	38.67934078143325	40.0	40.0	40.0	40.0	40.0
27	38.79422257885316	40.0	40.0	40.0	40.0	40.0
28	32.77061909758657	40.0	24.0	40.0	12.0	40.0
29	38.00883896055799	40.0	40.0	40.0	40.0	40.0
30	29.766014443552866	40.0	24.0	40.0	12.0	40.0
31	34.85602123325721	40.0	24.0	40.0	24.0	40.0
32	32.823257823591135	40.0	24.0	40.0	24.0	40.0
33	36.9892105425591	40.0	40.0	40.0	24.0	40.0
34	37.82082587494599	40.0	40.0	40.0	24.0	40.0
35	28.592457255724955	24.0	12.0	40.0	12.0	40.0
36	29.2068143941732	40.0	12.0	40.0	12.0	40.0
37	34.654799086476146	40.0	24.0	40.0	24.0	40.0
38	32.96386642799827	40.0	24.0	40.0	24.0	40.0
39	25.70912906610703	24.0	12.0	40.0	12.0	40.0
40	26.30995617554472	24.0	12.0	40.0	12.0	40.0
41	28.607542744275044	24.0	12.0	40.0	12.0	40.0
42	25.68861181408555	24.0	12.0	40.0	12.0	40.0
43	26.83649157459416	24.0	12.0	40.0	12.0	40.0
44	25.338411209184617	24.0	12.0	40.0	12.0	40.0
45	31.49378433430035	40.0	24.0	40.0	12.0	40.0
46	29.606937843343005	24.0	24.0	40.0	12.0	40.0
47	24.89542620825875	24.0	12.0	40.0	12.0	40.0
48	29.046478612431333	24.0	24.0	40.0	12.0	40.0
49	26.221023393617678	24.0	12.0	40.0	12.0	40.0
50	30.418023578791434	24.0	24.0	40.0	12.0	40.0
51	25.208987099561757	24.0	12.0	40.0	12.0	40.0
52	29.41314733658416	24.0	24.0	40.0	12.0	40.0
53	27.72539966668724	24.0	24.0	40.0	12.0	40.0
54	23.377742114684278	12.0	12.0	40.0	12.0	40.0
55	27.145188568606876	24.0	24.0	40.0	12.0	40.0
56	24.73931238812419	24.0	12.0	40.0	12.0	40.0
57	23.77806308252577	24.0	12.0	40.0	12.0	40.0
58	27.523659033393	24.0	24.0	40.0	12.0	40.0
59	26.390420344423184	24.0	24.0	40.0	12.0	40.0
60	25.003592370841307	24.0	12.0	40.0	12.0	40.0
61	26.925745324362694	24.0	24.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.006985197749585836
1101	2	-0.17400421043414838
1101	3	0.31747081443529623
1101	4	0.03735642202748579
1101	5	0.007694973605232747
1101	6	0.2136356856410231
1101	7	0.18444733339237018
1101	8	0.17934593434881663
1101	9	0.23768988280907877
1101	10	0.05514729717105382
1101	11	0.1172995355552402
1101	12	0.1134177900208897
1101	13	-0.47034472524739357
1101	14	-0.12343082779759129
1101	15	0.009979371406146242
1101	16	-0.032871858734758064
1101	17	-0.1938607029172985
1101	18	0.06715283098037617
1101	19	0.025860969834361924
1101	20	-0.32403642101029106
1101	21	-0.1795630828223267
1101	22	-0.58206885309896
1101	23	-0.03400054312007228
1101	24	0.020627709893616952
1101	25	-0.18985643676875696
1101	26	-0.25626996354707643
1101	27	-0.03143255022232694
1101	28	-0.7749712375875504
1101	29	-0.43914034297760196
1101	30	-0.7718305052322201
1101	31	-0.37490849325035214
1101	32	-0.5477896190883271
1101	33	-0.15940622997977982
1101	34	0.12264681794312793
1101	35	-0.44515814456795155
1101	36	-0.32036250632178565
1101	37	-0.5181007723402544
1101	38	-1.1158069562075426
1101	39	-0.6626211016448451
1101	40	-0.8574897537281707
1101	41	-0.8813898003720908
1101	42	-0.34423590206258226
1101	43	0.24706328563623714
1101	44	-0.2809849065579577
1101	45	-0.3579480404354456
1101	46	-0.7002312315272086
1101	47	-0.25195583947199296
1101	48	0.6859138759257704
1101	49	-0.43127504474799494
1101	50	-0.4586935805347139
1101	51	-0.5316386476888724
1101	52	0.1185550836493654
1101	53	-0.47674304164108605
1101	54	-0.08322353531018223
1101	55	0.09475573881270805
1101	56	0.36834661547767666
1101	57	-0.17654377840817048
1101	58	-0.19561677412249523
1101	59	-0.036450779683878665
1101	60	0.18533756202366192
1101	61	-0.36442986621112894
1102	1	0.13097713323345772
1102	2	-0.14754251688576403
1102	3	-0.33701507266147956
1102	4	-0.2925326908757384
1102	5	-0.1168009941367032
1102	6	-0.177745362746073
1102	7	-0.23188331176891808
1102	8	-0.26395043661892714
1102	9	-0.15041495590060094
1102	10	-0.23794746089346575
1102	11	-0.20049280315443951
1102	12	-0.11364874223717436
1102	13	-0.7818366607312655
1102	14	-0.5279167148943671
1102	15	-0.15332708020675767
1102	16	-0.4202206490573417
1102	17	-0.09255021904633054
1102	18	-0.08733305611639963
1102	19	-0.27605435274628576
1102	20	-0.32983279197803483
1102	21	-0.8365691312094228
1102	22	-0.7441152240667037
1102	23	-0.17235739795878402
1102	24	-0.14141866107412682
1102	25	-0.5643524045106929
1102	26	-0.06448568935352483
1102	27	-0.5135394050610387
1102	28	-0.5403442214585183
1102	29	-0.6047149397517941
1102	30	0.06965134960648811
1102	31	-0.21286212228260837
1102	32	-0.2332734900560709
1102	33	-0.5336501815926837
1102	34	-0.4592584239923525
1102	35	-0.18129725747117575
1102	36	0.8547887033556343
1102	37	-0.7789374658886388
1102	38	-0.603962198143023
1102	39	1.1770966402906389
1102	40	0.5948791978847332
1102	41	0.2398299576924252
1102	42	0.5539495818083857
1102	43	0.5315894953136571
1102	44	0.7943679160226864
1102	45	-0.6321415888225417
1102	46	-0.4698884895917246
1102	47	0.6721873056892989
1102	48	0.3467001662483504
1102	49	0.11660801976813318
1102	50	-0.6761834998895537
1102	51	0.2899339329562878
1102	52	-0.5964146744151506
1102	53	-0.04730755777011808
1102	54	0.7239841259801416
1102	55	0.07081420655464399
1102	56	0.057610728380900866
1102	57	0.8536981570757014
1102	58	0.16073403232911687
1102	59	0.1381963977354772
1102	60	0.05353312653979003
1102	61	-0.4360024468562891
1103	1	-0.05450673773428605
1103	2	0.10245748311423597
1103	3	-0.00636991137115217
1103	4	-0.17962946506929
1103	5	-0.09260744574960711
1103	6	-0.0648421369396246
1103	7	-0.47381879563988605
1103	8	-0.20749882371570294
1103	9	-0.2229956010618892
1103	10	-0.27020552540959386
1103	11	0.11402332587782382
1103	12	-0.11364874223717436
1103	13	0.01655043604292672
1103	14	-0.07630381166855926
1103	15	0.13699550043840247
1103	16	0.11203741545879353
1103	17	0.18970784546979758
1103	18	0.049763718077151964
1103	19	0.06265532467307366
1103	20	-0.5233811790748106
1103	21	-0.10269816346748684
1103	22	0.13491703399781585
1103	23	-0.11590578505555271
1103	24	0.15696843570006536
1103	25	-0.16112659805907725
1103	26	-0.16125988290191628
1103	27	-0.2393458566739426
1103	28	-0.2500216408133582
1103	29	1.2376992562224132E-4
1103	30	-1.0271228439418962
1103	31	-0.7612492190568005
1103	32	-0.6606928448947755
1103	33	0.06312401195570061
1103	34	0.3471931889108717
1103	35	-0.6329101606969836
1103	36	0.06446612271047414
1103	37	0.3017076954016815
1103	38	-0.14428477878819024
1103	39	-0.6212904564835533
1103	40	-1.2518949956636547
1103	41	0.4172493125311334
1103	42	-0.30895364399806624
1103	43	-0.871636311137955
1103	44	-0.7540191807515058
1103	45	-0.4869802984999616
1103	46	-0.24408203797882067
1103	47	-0.6826514039881211
1103	48	0.47573242431286644
1103	49	-0.23823068990928675
1103	50	-0.6600544676314897
1103	51	-0.1616789702695165
1103	52	0.14552080945581736
1103	53	-0.3698882029314099
1103	54	-0.5421449062779224
1103	55	-0.8969277289292279
1103	56	0.18664298644541688
1103	57	0.0633755764305377
1103	58	-0.34733048379991516
1103	59	-0.35373908613549077
1103	60	0.17450086847527402
1103	61	-0.3150347049208051
1104	1	-0.12644734193295193
1104	2	-0.22447544059287594
1104	3	-0.1633079554059762
1104	4	-0.12100171289314687
1104	5	-0.05753320099794479
1104	6	0.19962278881562412
1104	7	-0.6116836189885788
1104	8	-0.13799037517499357
1104	9	-0.18344106957187023
1104	10	0.029973686059939553
1104	11	-0.10653478984158227
1104	12	0.12073425827485806
1104	13	-0.1553645665172354
1104	14	0.022774529345262806
1104	15	-0.06244126351448642
1104	16	0.026527942852546005
1104	17	-0.04877141720301381
1104	18	-0.04457831981327587
1104	19	-0.023494188895796242
1104	20	0.0381907615293926
1104	21	-0.8718993923461369
1104	22	0.06835277388516658
1104	23	0.044744496562465486
1104	24	0.004382670211079187
1104	25	-0.22730683359415593
1104	26	-0.003169765134380498
1104	27	0.03689582279354653
1104	28	0.4555595163807027
1104	29	0.42779914114937867
1104	30	0.3088986614344478
1104	31	-0.001520596424953169
1104	32	0.710146888848179
1104	33	-0.3356215077575584
1104	34	0.28075693699074833
1104	35	-1.0851631048854102
1104	36	0.2896325333607557
1104	37	0.11942402924704965
1104	38	-0.705216678429764
1104	39	0.44898604121229724
1104	40	0.46827909547817725
1104	41	0.10682944565965613
1104	42	0.31201409793741774
1104	43	0.23230634119179427
1104	44	-0.08120812084367302
1104	45	0.44760751512011154
1104	46	0.7662866256156491
1104	47	0.23452729544864326
1104	48	-0.4929055685186725
1104	49	0.5168896377916887
1104	50	-0.037934651963286825
1104	51	0.34484944754922253
1104	52	-0.21341416238238153
1104	53	0.30599505359700885
1104	54	0.2861961075469601
1104	55	0.5988480007174708
1104	56	0.743222607541167
1104	57	0.4057974914330984
1104	58	0.023381241750520587
1104	59	0.24789941872882082
1104	60	0.3942909350395354
1104	61	-0.054410024941287105
1201	1	0.08719146017503476
1201	2	0.12397354801515803
1201	3	0.04987327467386393
1201	4	0.19582747073523876
1201	5	-0.02905328683214492
1201	6	0.01593128703267155
1201	7	0.3138583671895887
1201	8	0.09896882202277624
1201	9	0.13971523072160608
1201	10	0.044663325998094194
1201	11	-0.004796016134619663
1201	12	0.08786796397715335
1201	13	0.07258519522974183
1201	14	0.10897163244097641
1201	15	0.023771617927017985
1201	16	-0.022574269271537162
1201	17	-0.15357118999483532
1201	18	-0.0140629990395027
1201	19	0.00700535353217191
1201	20	-0.6334145273721177
1201	21	-0.01664993669906778
1201	22	0.3002155653901042
1201	23	-0.05358615445207704
1201	24	0.07388628345226778
1201	25	-0.2618929675838686
1201	26	0.09762230782469317
1201	27	0.24141474103015526
1201	28	-0.9892636083628972
1201	29	0.09874173952736953
1201	30	-0.5217839098047854
1201	31	0.10761822146137945
1201	32	-0.4081275912553224
1201	33	0.06342863582538882
1201	34	0.3008743278834842
1201	35	-0.10794703621805013
1201	36	0.0700295165410374
1201	37	0.6418923936637242
1201	38	0.7108425219236736
1201	39	-0.4930758730166147
1201	40	-0.38490339686932273
1201	41	0.09240803760007665
1201	42	-0.1134332181659623
1201	43	-0.23080388633195525
1201	44	-0.5398846331981169
1201	45	0.559049971492982
1201	46	0.3892630527670278
1201	47	1.8988114318997766
1201	48	0.4783778421285554
1201	49	-1.0284731063782147
1201	50	-0.0887724821251723
1201	51	-0.33587572522366216
1201	52	0.5843074444843559
1201	53	0.03580666014817879
1201	54	-0.12803280469388056
1201	55	0.9164661016357698
1201	56	-0.041087405397075116
1201	57	1.2731331599616098
1201	58	0.34846250023138126
1201	59	0.11012290321870566
1201	60	0.9298237697771405
1201	61	0.4204235135105421
1202	1	0.022610197749585836
1202	2	-0.041191710434148376
1202	3	-0.026279185564703766
1202	4	0.02173142202748579
1202	5	-0.7579300263947673
1202	6	0.07301068564102309
1202	7	-0.5811776666076298
1202	8	0.24965843434881663
1202	9	-0.12949761719092123
1202	10	0.22702229717105382
1202	11	0.2735495355552402
1202	12	0.0431052900208897
1202	13	-0.6890947252473936
1202	14	0.07969417220240871
1202	15	-0.005645628593853758
1202	16	-0.0016218587347580637
1202	17	0.2358267970827015
1202	18	0.09840283098037617
1202	19	-0.005389030165638076
1202	20	0.035338578989708935
1202	21	-0.1873755828223267
1202	22	-0.44925635309895995
1202	23	-0.02618804312007228
1202	24	-0.534059790106383
1202	25	-0.26798143676875696
1202	26	-0.5062699635470764
1202	27	0.24981744977767306
1202	28	-0.2515337375875504
1202	29	-0.04851534297760196
1202	30	0.3375444947677799
1202	31	0.46102900674964786
1202	32	0.9600228809116729
1202	33	-0.4094062299797798
1202	34	0.06014681794312793
1202	35	1.3517168554320484
1202	36	0.9608874936782144
1202	37	-0.06497577234025442
1202	38	1.8295055437924574
1202	39	1.3764413983551549
1202	40	1.2128227462718293
1202	41	0.4154851996279092
1202	42	1.1557640979374177
1202	43	0.8251882856362371
1202	44	1.1955775934420423
1202	45	0.1342394595645544
1202	46	0.7294562684727914
1202	47	0.505856660528007
1202	48	1.2874763759257704
1202	49	1.021849955252005
1202	50	0.5647439194652861
1202	51	1.7105488523111276
1202	52	0.5404300836493654
1202	53	0.976381958358914
1202	54	1.3855264646898178
1202	55	0.540068238812708
1202	56	1.1730341154776767
1202	57	0.9015812215918295
1202	58	1.4215707258775048
1202	59	1.3776117203161213
1202	60	1.529087562023662
1202	61	1.463695133788871
1203	1	0.1269528915865621
1203	2	0.21940098340282788
1203	3	0.3043464357076644
1203	4	0.06859089021833853
1203	5	0.4242270312593064
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2403	23	-0.17135129848069397
2403	24	-0.39842991681203443
2403	25	0.24944059052169365
2403	26	-0.40138753177319586
2403	27	-0.17725065451082855
2403	28	0.8312208920420758
2403	29	-0.223969168513662
2403	30	0.22410333979701846
2403	31	0.29073782741241416
2403	32	-0.3221560908232206
2403	33	0.08835510042957395
2403	34	-0.037121091413595764
2403	35	0.0145305737556356
2403	36	-0.2831884078812408
2403	37	0.7330414060223163
2403	38	-1.1467371462660232
2403	39	-0.15780872347720276
2403	40	-0.5563658502194002
2403	41	0.05475481463765419
2403	42	-0.3546525687292501
2403	43	0.5997527349539773
2403	44	-0.1349535956417789
2403	45	0.9846445521571496
2403	46	0.08555702383341668
2403	47	-0.4793407078930443
2403	48	-1.4227301299221828
2403	49	-0.8245194404594969
2403	50	0.46526779860758793
2403	51	-0.4238779264413104
2403	52	-0.0170900186898173
2403	53	0.13278424880725836
2403	54	-0.19876629846807603
2403	55	0.40029606532342754
2403	56	0.007494398128748969
2403	57	-1.899131497706417
2403	58	-0.9973650684694739
2403	59	-1.850123416136121
2403	60	-1.516736633882772
2403	61	-0.0028923418446566984
2404	1	0.14191736829221924
2404	2	0.056070886465079184
2404	3	0.04839377955157431
2404	4	0.27360884450810374
2404	5	0.4852870278688002
2404	6	0.1778435344782352
2404	7	0.3135655504466328
2404	8	-0.04164486022482805
2404	9	0.31545151071605204
2404	10	0.09069720027182626
2404	11	0.114210872764545
2404	12	0.41217021250150765
2404	13	0.14714558483012752
2404	14	0.19276345514814608
2404	15	0.02984371249142015
2404	16	0.34509568002493296
2404	17	0.10737476219897957
2404	18	0.23412230772456155
2404	19	0.03833674502816109
2404	20	0.6385968154238171
2404	21	1.0228957350071326
2404	22	0.4491205848855344
2404	23	0.43529451501946426
2404	24	0.04406520989361695
2404	25	1.107503059355274
2404	26	0.19764234265447556
2404	27	0.14837607380868434
2404	28	1.2325990143504342
2404	29	0.1994861105107688
2404	30	1.0647126149228185
2404	31	0.8535919912457715
2404	32	0.3882568925395802
2404	33	0.23672991343106986
2404	34	0.10744575205165319
2404	35	0.5844566810134424
2404	36	0.005763947166585126
2404	37	0.2945809137062554
2404	38	-0.1380332158974653
2404	39	0.09682657277376094
2404	40	0.3012432889074894
2404	41	0.508084521333334
2404	42	-0.4554277625276981
2404	43	-0.2341019275420564
2404	44	-0.07234876314710448
2404	45	0.15949382002967027
2404	46	0.4789112103332549
2404	47	-0.18721504489834828
2404	48	-1.025205310120743
2404	49	0.3304739862597579
2404	50	0.16915283419396943
2404	51	-0.25814058567336673
2404	52	-0.17280877293978136
2404	53	0.1177337025449603
2404	54	0.767369972441756
2404	55	0.15477269617704792
2404	56	-0.34574010932852417
2404	57	-1.3911755613539079
2404	58	-0.11264681288218625
2404	59	0.3169285807812372
2404	60	0.532902969000407
2404	61	-0.07397443985453833
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	30.0
16	108.0
17	197.0
18	226.0
19	256.0
20	368.0
21	437.0
22	629.0
23	871.0
24	1347.0
25	2002.0
26	3318.0
27	6429.0
28	17945.0
29	25639.0
30	17387.0
31	9408.0
32	4951.0
33	2968.0
34	2657.0
35	3563.0
36	4422.0
37	6004.0
38	10013.0
39	26442.0
40	14390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.631108532347367	66.88167795218725	13.164863246649835	11.322350268815546
2	53.586815628664894	8.392691809147584	23.63866427998272	14.381828282204804
3	9.765446577371767	53.56582926979816	12.481328313067094	24.187395839762978
4	8.713042404789828	8.932164681192518	68.86982285044134	13.484970063576323
5	8.180359237084131	8.929695697796431	13.045491019072896	69.84445404604655
6	53.991111659774084	9.134621319671625	23.26893401641874	13.605333004135547
7	53.65779890130239	10.377754459601258	11.985679896302697	23.978766742793656
8	9.430282081353003	54.449725325597186	24.075674341090057	12.044318251959755
9	9.208690821554224	55.98049503117092	11.77951978272946	23.0312943645454
10	53.99481513486821	22.406641565335473	11.082649219184	12.515894080612307
11	9.774705265107093	65.78667983457811	11.269057465588544	13.16955743472625
12	55.36016295290415	11.502376396518734	10.233318930930189	22.904141719646937
13	10.616011357323622	22.081353002901054	11.402999814826245	55.89963582494908
14	55.79223504721931	21.176470588235293	10.800567866181101	12.230726498364298
15	10.279612369606815	11.854823776310104	55.89963582494908	21.965928029134005
16	11.012900438244552	22.21344361459169	11.311030183322018	55.462625763841736
17	55.96197765570027	20.953644836738473	11.52336275538547	11.56101475217579
18	11.679525955187952	11.719029689525339	55.67434109005617	20.92710326523054
19	56.870563545460165	21.785074995370657	11.218443305968767	10.12591815320042
20	12.415900253070799	21.725202148015555	54.426269983334365	11.432627615579285
21	13.673847293376953	55.234862045552745	10.743781248071107	20.347509412999198
22	68.72415283007221	11.140670328992037	10.334547250169743	9.800629590766002
23	67.98098882785013	12.803530646256403	10.030862292451083	9.18461823344238
24	58.7093389296957	22.224554039874082	9.885192272081971	9.180914758348251
25	25.22004814517622	55.967532868341465	9.635824949077218	9.1765940374051
26	24.04481204863897	57.78408740201222	9.882106042836861	8.288994506511942
27	14.120116042219616	66.30208011851121	10.43392383186223	9.14388000740695
28	25.63051663477563	55.27806925498426	9.975310166039133	9.116103944200974
29	25.43423245478674	56.3076353311524	10.001234491698044	8.256897722362817
30	17.418677859391394	63.534349731498054	10.410468489599408	8.636503919511142
31	25.182396148385898	12.523301030800567	54.007777297697665	8.286525523115857
32	69.20066662551694	12.917103882476392	10.302450466020616	7.57977902598605
33	14.56171646904146	22.803952915614758	54.75010339059423	7.884227224749547
34	14.967594592926362	23.651009196963148	53.677550768471086	7.703845441639405
35	14.497253255971854	23.276958212456023	12.470217887784704	49.75557064378742
36	16.113202888710575	22.73563360286402	51.43139312388124	9.719770384544164
37	26.342818344546632	12.978211221529534	53.387445219430894	7.291525214492934
38	16.584778717363125	23.074501573976917	8.938954385531757	51.4017653231282
39	39.463613357200174	14.005925560150608	29.79630886982285	16.73415221282637
40	15.949632738719831	47.59459292636257	8.6581075242269	27.797666810690696
41	25.024998456885378	17.1310412937473	8.91056107647676	48.93339917289056
42	18.55008950064811	63.37139682735634	9.889512993025122	8.189000678970434
43	24.444787358805012	14.689833960866613	50.64502191222764	10.220356768100734
44	20.895006481081417	24.013949756187888	47.71001790012962	7.381025862601074
45	14.977470526510709	22.067156348373558	54.8774766989692	8.077896426146534
46	24.763903462749212	11.99370409233998	10.991914079377816	52.25047836553299
47	23.886797111289425	22.539966668724155	11.959138324794766	41.614097895191655
48	13.45040429603111	54.44355286710697	22.396148385902105	9.709894450959817
49	14.023825689772238	10.727732855996544	12.799827171162272	62.44861428306895
50	23.92444910807975	10.70057403863959	12.522066539102525	52.85291031417814
51	22.98808715511388	12.086908215542252	14.902166532930067	50.0228380964138
52	13.325720634528732	22.59860502438121	11.867785939139559	52.2078884019505
53	13.646071230170978	19.966051478303807	10.345040429603111	56.042836861922105
54	27.655700265415717	11.054255910129005	10.332695512622678	50.957348311832604
55	13.527560027158817	20.80427134127523	53.4929942596136	12.17517437195235
56	23.736189124128142	11.335720017282885	53.528177273007834	11.399913585581137
57	49.14881797419912	13.11647429171039	17.577927288438985	20.156780445651503
58	20.774026294673167	12.659712363434355	56.576137275476825	9.990124066415653
59	10.52280723412135	13.319548176038515	21.568421702364052	54.58922288747608
60	10.553052280723412	11.175236096537251	48.30133942349238	29.97037219924696
61	19.644466390963522	12.210357385346583	12.126411949879637	56.01876427381026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	1.0
12	2.5
13	3.0
14	4.5
15	6.0
16	12.5
17	21.5
18	24.0
19	32.0
20	47.5
21	55.0
22	71.5
23	88.0
24	88.5
25	121.0
26	153.0
27	162.5
28	172.0
29	270.0
30	411.5
31	455.0
32	521.5
33	588.0
34	809.5
35	1061.5
36	1092.0
37	1178.5
38	1265.0
39	1429.5
40	1729.5
41	1865.0
42	2409.5
43	3330.0
44	3706.0
45	5596.0
46	7486.0
47	14433.0
48	35328.5
49	49277.0
50	38495.5
51	27714.0
52	21397.5
53	12096.0
54	9111.0
55	7376.0
56	5641.0
57	4454.5
58	2719.5
59	2171.0
60	1750.5
61	1173.0
62	1016.0
63	911.5
64	807.0
65	699.5
66	513.5
67	435.0
68	370.5
69	306.0
70	269.5
71	198.5
72	164.0
73	140.5
74	117.0
75	106.0
76	70.0
77	45.0
78	33.5
79	22.0
80	21.0
81	17.0
82	14.0
83	11.5
84	7.5
85	6.0
86	7.0
87	8.0
88	5.5
89	6.0
90	9.0
91	6.0
92	3.0
93	6.0
94	8.5
95	8.0
96	10.5
97	13.0
98	12.0
99	23.5
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001851737547064996
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.001851737547064996
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	162010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.63465218196407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.74456799591002	34.28615517560644
2	5.692420756646217	4.398493920128387
3	1.7622060327198363	2.042466514412691
4	0.8148006134969324	1.2591815320041972
5	0.4265720858895705	0.8240232084439232
6	0.2987602249488752	0.6925498426023086
7	0.2364519427402863	0.6394666995864453
8	0.18532719836400818	0.5728041478921054
9	0.15177658486707565	0.5277452009135238
>10	1.3452198364008179	11.256095302759089
>50	0.21248721881390592	5.565088574779335
>100	0.09266359918200409	7.56866860070366
>500	0.015976482617586912	4.096660699956793
>1k	0.017574130879345604	13.756558237145855
>5k	0.0015976482617586915	3.393617677921116
>10k+	0.0015976482617586915	9.120424665144126
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	14776	9.120424665144126	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCCACCCCCCTTGTCTC	5498	3.393617677921116	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCCACCCCCCTTGTCTC	4978	3.07264983642985	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCCACCCCCCTTGTCTC	3223	1.9893833713968272	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATTTCCACCCCCCTTGTCTC	2073	1.2795506450219123	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATTTCCACCCCCCTTGTCTC	1977	1.2202950435158324	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCCACCCCCCTTGTCTC	1807	1.1153632491821492	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCCACCCCCCTTGTCCC	1752	1.0814147274859576	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCCACCCCCCTTGTCCC	1538	0.9493241157953213	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCGACCCCCCTTGTCTC	1316	0.8122955373125116	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCCACCCCCCTTTTCTC	1220	0.7530399358064317	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCGACCCCCCTTGTCTC	1204	0.7431640022220851	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCCACCCCCCTTGTCCC	1199	0.7400777729769767	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCCACCCCCCTTTTCCC	993	0.6129251280785137	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCGACCCCCCTTGTCTC	971	0.599345719400037	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCCACCCCCCTTTTCTC	769	0.47466205789766064	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCCACCCCCCTTGTCCC	640	0.3950373433738658	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATGTCCACCCCCCTTGTCTC	590	0.3641750509227825	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCCACCCCCCTTTTCCC	575	0.35491636318745756	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATTTCCACCCCCCTTGTCCC	538	0.332078266773656	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATGTCCACCCCCCTTGTCTC	529	0.32652305413246097	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATTTCCACCCCCCTTGTCCC	523	0.322819579038331	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATTTCGACCCCCCTTGTCTC	509	0.31417813715202764	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCCACCCCCGTTGTCTC	500	0.3086229245108327	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATTTCCACCCCCCTTTTCTC	494	0.3049194494167027	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCGACCCCCCTTGTCTC	487	0.300598728473551	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCCACCCCCGTTGTCTC	459	0.2833158447009444	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATTTCGACCCCCCTTGTCTC	456	0.28146410715387943	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATGTCCACCCCCCTTGTCTC	402	0.24813283130670943	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCCACCCCCGTTGTCTC	345	0.21294981791247455	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATTTCCACCCCCCTTTTCCC	334	0.20616011357323621	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATTTCCACCCCCCTTTTCTC	331	0.20430837602617125	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCGACCCCCGTTGTCTC	327	0.20183939263008457	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATGTCCACCCCCCTTGTCTC	304	0.18764273810258625	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	300	0.1851737547064996	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCCACCCCCCTTTTCTC	297	0.18332201715943458	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCGACCCCCGTTGTCTC	291	0.17961854206530461	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCCACCCCCCTTTTCCC	272	0.16789087093389296	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGTGTA	270	0.16665637923584964	Illumina Single End PCR Primer 1 (100% over 21bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATGTCGACCCCCCTTGTCTC	268	0.16542188753780632	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCGTTTCCACCCCCCTTGTCTC	264	0.16295290414171965	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATTTCCACCCCCCTTTTCCC	242	0.149373495463243	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATGTCCACCCCCCTTGTCTC	225	0.1388803160298747	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCCACCCCCCTTTTCTC	225	0.1388803160298747	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCCACCCCCCTTTTCCC	218	0.13455959508672305	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATGTCGACCCCCCTTGTCTC	213	0.1314733658416147	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATGTCCACCCCCCTTGTCTC	207	0.12776989074748474	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCGACCCCCGTTGTCTC	199	0.1228319239553114	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCACTTCCACCCCCCTTGTCTC	195	0.12036294055922474	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	195	0.12036294055922474	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCACTTCCACCCCCCTTGTCTC	188	0.11604221961607308	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACACTTCCACCCCCCTTGTCTC	176	0.1086352694278131	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCCACCCCCGTTGTCTC	170	0.1049317943336831	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGAAATTTCCACCCCCCTTGTCTC	165	0.10184556508857479	Illumina Single End PCR Primer 1 (96% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	44.47441515955805	0.0	0.0	0.0	0.0
2	44.66144065181162	0.0	0.0	0.0	0.0
3	44.73489290784519	0.0	0.0	0.0	0.0
4	44.80032096784149	0.0	0.0	0.0	0.0
5	44.912659712363435	0.0	0.0	0.0	0.0
6	45.06758842046787	0.0	0.0	0.0	0.0
7	45.262020862909694	0.0	0.0	0.0	0.0
8	45.74223813344855	0.0	0.0	0.0	0.0
9	46.0625887290908	0.0	0.0	0.0	0.0
10	46.45762607246466	0.0	0.0	0.0	0.0
11	46.84834269489538	0.0	0.0	0.0	0.0
12	47.36312573297945	0.0	0.0	0.0	0.0
13	47.986544040491324	0.0	0.0	0.0	0.0
14	48.69390778347016	0.0	0.0	0.0	0.0
15	49.528424171347446	0.0	0.0	0.0	0.0
16	50.62527004505895	0.0	0.0	0.0	0.0
17	51.362261588790815	0.0	0.0	0.0	0.0
18	52.30788222949201	0.0	0.0	0.0	0.0
19	52.926362570211715	0.0	0.0	0.0	0.0
20	53.623233133757175	0.0	0.0	0.0	0.0
21	54.279982717116226	0.0	0.0	0.0	0.0
22	54.62749213011543	0.0	0.0	0.0	0.0
23	55.223751620270356	0.0	0.0	0.0	0.0
24	55.91691870872168	0.0	0.0	0.0	0.0
25	56.3162767730387	0.0	0.0	0.0	0.0
26	56.83414604036788	0.0	0.0	0.0	0.0
27	57.273625084871306	0.0	0.0	0.0	0.0
28	57.81062897352015	0.0	0.0	0.0	0.0
29	58.3846676131103	6.172458490216654E-4	0.0	0.0	0.0
30	59.02907227948892	6.172458490216654E-4	0.0	0.0	0.0
31	59.804950311709156	6.172458490216654E-4	0.0	0.0	0.0
32	60.59132152336276	6.172458490216654E-4	0.0	0.0	0.0
33	61.466576137275474	6.172458490216654E-4	0.0	0.0	0.0
34	62.25109561138201	6.172458490216654E-4	0.0	0.0	0.0
35	62.94179371643726	0.003703475094129992	0.0	0.0	0.0
36	63.589284612060986	0.004937966792173323	0.0	0.0	0.0
37	64.16764397259429	0.004937966792173323	0.0	0.0	0.0
38	64.52873279427196	0.006172458490216653	0.0	0.0	0.0
39	64.900314795383	0.00925868773532498	0.0	0.0	0.0
40	65.25029319177828	0.011110425282389976	0.0	0.0	0.0
41	65.9656811307944	0.011110425282389976	0.0	0.0	0.0
42	66.47058823529412	0.011110425282389976	0.0	0.0	0.0
43	66.91500524658971	0.011110425282389976	0.0	0.0	0.0
44	68.14085550274675	0.011110425282389976	0.0	0.0	0.0
45	78.54576877970496	0.011110425282389976	0.0	0.0	0.0
46	79.07598296401457	0.011110425282389976	0.0	0.0	0.0
47	79.43275106474908	0.011110425282389976	0.0	0.0	0.0
48	79.86235417566817	0.011110425282389976	0.0	0.0	0.0
49	80.26911919017344	0.011110425282389976	0.0	0.0	0.0
50	80.75242268995741	0.011110425282389976	0.0	0.0	0.0
>>END_MODULE