AM-AL-EC05_S55_R2_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	AM-AL-EC05_S55_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162010
Sequences flagged as poor quality	0
Sequence length	61
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	14776	9.120424665144126	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCCACCCCCCTTGTCTC	5498	3.393617677921116	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCCACCCCCCTTGTCTC	4978	3.07264983642985	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCCACCCCCCTTGTCTC	3223	1.9893833713968272	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATTTCCACCCCCCTTGTCTC	2073	1.2795506450219123	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATTTCCACCCCCCTTGTCTC	1977	1.2202950435158324	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCCACCCCCCTTGTCTC	1807	1.1153632491821492	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCCACCCCCCTTGTCCC	1752	1.0814147274859576	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCCACCCCCCTTGTCCC	1538	0.9493241157953213	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCGACCCCCCTTGTCTC	1316	0.8122955373125116	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCCACCCCCCTTTTCTC	1220	0.7530399358064317	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCGACCCCCCTTGTCTC	1204	0.7431640022220851	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCCACCCCCCTTGTCCC	1199	0.7400777729769767	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCCACCCCCCTTTTCCC	993	0.6129251280785137	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCGACCCCCCTTGTCTC	971	0.599345719400037	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCCACCCCCCTTTTCTC	769	0.47466205789766064	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCCACCCCCCTTGTCCC	640	0.3950373433738658	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATGTCCACCCCCCTTGTCTC	590	0.3641750509227825	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCCACCCCCCTTTTCCC	575	0.35491636318745756	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATTTCCACCCCCCTTGTCCC	538	0.332078266773656	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATGTCCACCCCCCTTGTCTC	529	0.32652305413246097	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATTTCCACCCCCCTTGTCCC	523	0.322819579038331	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATTTCGACCCCCCTTGTCTC	509	0.31417813715202764	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCCACCCCCGTTGTCTC	500	0.3086229245108327	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATTTCCACCCCCCTTTTCTC	494	0.3049194494167027	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCGACCCCCCTTGTCTC	487	0.300598728473551	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCCACCCCCGTTGTCTC	459	0.2833158447009444	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATTTCGACCCCCCTTGTCTC	456	0.28146410715387943	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATGTCCACCCCCCTTGTCTC	402	0.24813283130670943	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCCACCCCCGTTGTCTC	345	0.21294981791247455	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATTTCCACCCCCCTTTTCCC	334	0.20616011357323621	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATTTCCACCCCCCTTTTCTC	331	0.20430837602617125	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCGACCCCCGTTGTCTC	327	0.20183939263008457	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATGTCCACCCCCCTTGTCTC	304	0.18764273810258625	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	300	0.1851737547064996	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCCACCCCCCTTTTCTC	297	0.18332201715943458	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATTTCGACCCCCGTTGTCTC	291	0.17961854206530461	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATTTCCACCCCCCTTTTCCC	272	0.16789087093389296	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGTGTA	270	0.16665637923584964	Illumina Single End PCR Primer 1 (100% over 21bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGACATGTCGACCCCCCTTGTCTC	268	0.16542188753780632	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCGTTTCCACCCCCCTTGTCTC	264	0.16295290414171965	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACATTTCCACCCCCCTTTTCCC	242	0.149373495463243	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATGTCCACCCCCCTTGTCTC	225	0.1388803160298747	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCCACCCCCCTTTTCTC	225	0.1388803160298747	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCCACCCCCCTTTTCCC	218	0.13455959508672305	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTACATGTCGACCCCCCTTGTCTC	213	0.1314733658416147	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCATGTCCACCCCCCTTGTCTC	207	0.12776989074748474	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCATTTCGACCCCCGTTGTCTC	199	0.1228319239553114	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCTCCACTTCCACCCCCCTTGTCTC	195	0.12036294055922474	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	195	0.12036294055922474	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCCCACTTCCACCCCCCTTGTCTC	188	0.11604221961607308	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCCACACTTCCACCCCCCTTGTCTC	176	0.1086352694278131	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGCCATTTCCACCCCCGTTGTCTC	170	0.1049317943336831	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTCTTCGAAATTTCCACCCCCCTTGTCTC	165	0.10184556508857479	Illumina Single End PCR Primer 1 (96% over 29bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content