##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-EC05_S55_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162010
Sequences flagged as poor quality	0
Sequence length	61
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.388679711128944	40.0	40.0	40.0	40.0	40.0
2	39.14899080303685	40.0	40.0	40.0	40.0	40.0
3	38.9878896364422	40.0	40.0	40.0	40.0	40.0
4	38.80991296833529	40.0	40.0	40.0	40.0	40.0
5	39.340065428059994	40.0	40.0	40.0	40.0	40.0
6	39.037096475526205	40.0	40.0	40.0	40.0	40.0
7	39.24387383494846	40.0	40.0	40.0	40.0	40.0
8	39.08284673785569	40.0	40.0	40.0	40.0	40.0
9	39.252614036170606	40.0	40.0	40.0	40.0	40.0
10	39.2563422010987	40.0	40.0	40.0	40.0	40.0
11	39.35989136473057	40.0	40.0	40.0	40.0	40.0
12	39.358014937349544	40.0	40.0	40.0	40.0	40.0
13	39.08896981667798	40.0	40.0	40.0	40.0	40.0
14	39.33539904944139	40.0	40.0	40.0	40.0	40.0
15	39.471291895562004	40.0	40.0	40.0	40.0	40.0
16	39.09733967039072	40.0	40.0	40.0	40.0	40.0
17	39.52242454169496	40.0	40.0	40.0	40.0	40.0
18	39.43363989877168	40.0	40.0	40.0	40.0	40.0
19	38.86337880377754	40.0	40.0	40.0	40.0	40.0
20	39.14859576569348	40.0	40.0	40.0	40.0	40.0
21	39.473736189124125	40.0	40.0	40.0	40.0	40.0
22	38.595024998456886	40.0	40.0	40.0	40.0	40.0
23	38.60875254613913	40.0	40.0	40.0	40.0	40.0
24	39.342238133448554	40.0	40.0	40.0	40.0	40.0
25	39.456033578174186	40.0	40.0	40.0	40.0	40.0
26	39.3772236281711	40.0	40.0	40.0	40.0	40.0
27	39.30693167088452	40.0	40.0	40.0	40.0	40.0
28	39.27150175915067	40.0	40.0	40.0	40.0	40.0
29	39.315573112770814	40.0	40.0	40.0	40.0	40.0
30	39.24160237022406	40.0	40.0	40.0	40.0	40.0
31	39.258539596321214	40.0	40.0	40.0	40.0	40.0
32	39.32154805258935	40.0	40.0	40.0	40.0	40.0
33	39.15039812357262	40.0	40.0	40.0	40.0	40.0
34	39.01892475773101	40.0	40.0	40.0	40.0	40.0
35	39.28394543546695	40.0	40.0	40.0	40.0	40.0
36	39.12506635392877	40.0	40.0	40.0	40.0	40.0
37	39.04176285414481	40.0	40.0	40.0	40.0	40.0
38	38.50621566569965	40.0	40.0	40.0	40.0	40.0
39	37.70411702981298	40.0	40.0	40.0	40.0	40.0
40	38.94947225479908	40.0	40.0	40.0	40.0	40.0
41	38.88927843960249	40.0	40.0	40.0	40.0	40.0
42	39.02480093821369	40.0	40.0	40.0	40.0	40.0
43	38.95465711993087	40.0	40.0	40.0	40.0	40.0
44	39.04193568298253	40.0	40.0	40.0	40.0	40.0
45	37.50499351891859	40.0	40.0	40.0	24.0	40.0
46	38.22768964878711	40.0	40.0	40.0	40.0	40.0
47	38.72304178754398	40.0	40.0	40.0	40.0	40.0
48	38.88466144065181	40.0	40.0	40.0	40.0	40.0
49	38.76837232269613	40.0	40.0	40.0	40.0	40.0
50	38.68637738411209	40.0	40.0	40.0	40.0	40.0
51	38.54836121227085	40.0	40.0	40.0	40.0	40.0
52	38.411949879637056	40.0	40.0	40.0	40.0	40.0
53	38.18608727856305	40.0	40.0	40.0	40.0	40.0
54	37.86203320782668	40.0	40.0	40.0	24.0	40.0
55	37.60930806740325	40.0	40.0	40.0	24.0	40.0
56	37.29117955681748	40.0	40.0	40.0	24.0	40.0
57	36.724868835257084	40.0	40.0	40.0	24.0	40.0
58	36.10833899142028	40.0	40.0	40.0	24.0	40.0
59	34.343559039565456	40.0	24.0	40.0	24.0	40.0
60	33.77487809394482	40.0	24.0	40.0	24.0	40.0
61	31.63103512128881	40.0	24.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.15791052653040083
1101	2	-0.026905638011491817
1101	3	0.09156953096850629
1101	4	-0.4752734585595988
1101	5	-0.12913449620059936
1101	6	0.17300760330401488
1101	7	-0.11126225470918882
1101	8	0.09277003489300739
1101	9	0.06027133773999793
1101	10	-0.07215607126548207
1101	11	-0.25400680765583417
1101	12	0.002642771255750631
1101	13	0.10104023157219189
1101	14	-0.08035960794703101
1101	15	-0.06337575877664392
1101	16	0.0878844296027026
1101	17	0.23656810709611875
1101	18	-0.0586454061320012
1101	19	0.36960611981528757
1101	20	0.17037611827277033
1101	21	0.1726271855963688
1101	22	0.21920058977201506
1101	23	-0.004382108982284194
1101	24	-0.22645170230345002
1101	25	-0.03172999430277201
1101	26	-0.24239179291745927
1101	27	-0.08135089252006367
1101	28	-0.012647333966377516
1101	29	0.14848316210018453
1101	30	-0.24841419244455665
1101	31	0.08674110972097537
1101	32	0.1167574632369508
1101	33	-0.03115649112972818
1101	34	0.31668828428824725
1101	35	0.29195325411770057
1101	36	-0.04491294279938529
1101	37	-0.21873745496093733
1101	38	-0.39272561411681295
1101	39	1.2564898665911528
1101	40	0.0120278056826848
1101	41	-0.16924393265839655
1101	42	-0.09866358978542422
1101	43	-0.06180620161933348
1101	44	-0.1283169541132665
1101	45	-0.10381113137385967
1101	46	-0.2533139995367577
1101	47	0.1716144104699282
1101	48	-0.13517529574932752
1101	49	-0.11622841001292983
1101	50	-0.28092959255453565
1101	51	-0.24392272520683633
1101	52	0.010063676927238419
1101	53	-0.17417457896549138
1101	54	-0.5044809801049581
1101	55	-0.5974251135918038
1101	56	-0.9337032981539011
1101	57	-0.24053178039561374
1101	58	-0.6195178879526893
1101	59	-0.2783953751740853
1101	60	0.2851185114089603
1101	61	-1.057234425456656
1102	1	-0.14602092508250308
1102	2	-0.014304831559876163
1102	3	-0.14759377548310937
1102	4	-0.1481565230757269
1102	5	-0.3151223994264072
1102	6	-0.247859332179857
1102	7	-0.3738630611608045
1102	8	-0.4321795618811848
1102	9	-0.1136197912922583
1102	10	-0.1149988132009625
1102	11	-0.2570310012042185
1102	12	-0.15436327713134546
1102	13	-0.6918025103632885
1102	14	-0.31347452730187086
1102	15	-0.10722656522825957
1102	16	-0.32567403813923335
1102	17	-0.198663747742593
1102	18	-0.4580909706481293
1102	19	-0.12182533179761634
1102	20	-0.5859242849530375
1102	21	-0.45111273375847105
1102	22	-0.6815050553892732
1102	23	-0.0956119476919639
1102	24	-0.14101823456151408
1102	25	-0.2512360426898681
1102	26	-0.21340993807874753
1102	27	-0.1866936344555441
1102	28	-0.012143301708313459
1102	29	-0.21492409596433504
1102	30	0.09861201723285973
1102	31	-0.2723818741499926
1102	32	-0.25673043998885703
1102	33	-0.0019226201619844119
1102	34	-0.010932683453688696
1102	35	-0.22190763297907523
1102	36	-0.5101347169929369
1102	37	-0.27292092270287327
1102	38	-0.5409110979877809
1102	39	1.2161672859459927
1102	40	-0.29669195238183477
1102	41	-0.23502014233581292
1102	42	-0.27759504139832814
1102	43	-0.17017313710320536
1102	44	-0.2838109057261704
1102	45	-1.1789119378254753
1102	46	0.01306704885033838
1102	47	-0.2026295411429757
1102	48	-0.09535674736223143
1102	49	-0.28936349065809
1102	50	-0.23556668932872782
1102	51	-0.20864046714231677
1102	52	-0.3467911617824413
1102	53	-0.6739225628364593
1102	54	-0.6869406575243104
1102	55	-0.29727390391438746
1102	56	-0.026445233637772958
1102	57	-1.1122555707181974
1102	58	-0.40354006537204157
1102	59	-1.1637280364644056
1102	60	-1.030405682139424
1102	61	-0.10410942545665591
1103	1	0.05559197814330474
1103	2	0.06634032973044413
1103	3	-0.1879163561282695
1103	4	-0.18847910372088705
1103	5	-0.03286433491027907
1103	6	-0.013988364437921064
1103	7	0.1180724227101635
1103	8	0.29362688973171913
1103	9	-0.0571681783890341
1103	10	0.01403344486354996
1103	11	0.25909803105384555
1103	12	-0.13016972874424937
1103	13	-0.4014799297181284
1103	14	0.07362224689168073
1103	15	0.021805692836259993
1103	16	-0.15631919942955363
1103	17	0.035207219999342954
1103	18	-0.13551032548684105
1103	19	-0.25892210599116083
1103	20	-0.3843113817272297
1103	21	0.04082275011249692
1103	22	0.09268849299782289
1103	23	-0.36174097995002796
1103	24	-0.04424404101312973
1103	25	-0.017365074947932158
1103	26	-0.011797034852939703
1103	27	-0.09798395703619178
1103	28	-0.012143301708313459
1103	29	-0.33589183789981547
1103	30	-0.09493636986390896
1103	31	-0.046575422537088684
1103	32	0.09004375355953442
1103	33	-0.22772907177488833
1103	34	-0.05931978022788087
1103	35	-0.06061731039842755
1103	36	0.17534915397480688
1103	37	-0.11163060012222559
1103	38	-0.10542722702003715
1103	39	1.2726188988492169
1103	40	-0.17572421044634723
1103	41	-0.009213690722916112
1103	42	0.028856571504896067
1103	43	0.21692363709034623
1103	44	0.054898771693181914
1103	45	-0.3966538733093401
1103	46	0.02113156497937041
1103	47	0.11188658788928052
1103	48	0.09012712360551234
1103	49	0.12999134805158974
1103	50	-0.24363120545775985
1103	51	-0.16831788649715662
1103	52	-0.08872664565340926
1103	53	0.10027098555063674
1103	54	0.18402708441117
1103	55	-0.11179003294664369
1103	56	0.2719418631364192
1103	57	-0.14451363523432548
1103	58	0.10452445075699046
1103	59	0.1104655119226905
1103	60	1.0824975436670243
1103	61	0.5813744455110879
1104	1	0.016293463038337563
1104	2	0.14596142548057145
1104	3	0.21198123731771545
1104	4	-0.1039936966548396
1104	5	0.049188916497811874
1104	6	0.17623180965322405
1104	7	0.10475957068763364
1104	8	0.15985832854379822
1104	9	0.27232490916856733
1104	10	0.06549273825832813
1104	11	0.09767970028067197
1104	12	0.27062392204940267
1104	13	0.15138745379441332
1104	14	0.17794892379900062
1104	15	-0.009556314332201055
1104	16	-0.10767608627031677
1104	17	0.23272386106437182
1104	18	0.1694051891060937
1104	19	-0.3404633246291553
1104	20	0.2863235389076877
1104	21	-0.07873690170521996
1104	22	0.12917082786725587
1104	23	-0.28736822009339846
1104	24	-0.04155586897011432
1104	25	-0.07228058954086691
1104	26	0.0774246753365091
1104	27	-0.014138590932759598
1104	28	-0.040177095871136714
1104	29	0.0609335589255835
1104	30	0.0056780694602025505
1104	31	0.1084424986098611
1104	32	0.20281897117345693
1104	33	0.08243477871154425
1104	34	0.13960495095491154
1104	35	-0.016454483977540235
1104	36	0.12299380323236164
1104	37	0.24666433075334737
1104	38	-0.04897561411681295
1104	39	1.169188279289564
1104	40	0.0347212580636338
1104	41	0.35791380543684426
1104	42	0.10694156894473394
1104	43	-0.023859773047902877
1104	44	-0.12149651760532976
1104	45	-0.21616232185004947
1104	46	-0.0706503090605679
1104	47	-0.036346899053882
1104	48	-0.12351854971758058
1104	49	-0.11412027509229716
1104	50	0.3048838995089582
1104	51	0.15042251288840447
1104	52	0.2868493912129537
1104	53	0.4053145083360974
1104	54	0.508911877037896
1104	55	0.2129667514875635
1104	56	0.48494749549688976
1104	57	0.6956041323027975
1104	58	0.006598183475880148
1104	59	0.25421871212750347
1104	60	0.40367009871054904
1104	61	0.3879540666068344
1201	1	0.0867437770274222
1201	2	0.045193020040194654
1201	3	0.3614968421017082
1201	4	-0.04283682834090996
1201	5	-0.04740611647893189
1201	6	0.01560824942727379
1201	7	-0.06357960063364487
1201	8	0.21412005974390524
1201	9	0.08061800772011907
1201	10	-0.18240085257760796
1201	11	0.02513086630042949
1201	12	-0.11973235299872442
1201	13	0.2532208975761705
1201	14	-0.1172165910484253
1201	15	0.06090977800268149
1201	16	0.0573022973959425
1201	17	0.14475958323925653
1201	18	0.08112534436502017
1201	19	-0.12862325394216612
1201	20	-0.08063344932165961
1201	21	0.1427595166102833
1201	22	-0.42204568259379016
1201	23	-0.3780463733958044
1201	24	0.2434010561458564
1201	25	-0.08936853804034683
1201	26	-0.003155336655034091
1201	27	-0.11095452075068124
1201	28	0.05373561831990514
1201	29	0.004068400668771233
1201	30	0.23482698548784953
1201	31	0.09132299341878536
1201	32	0.05869956065245674
1201	33	0.02531716692195829
1201	34	0.15385279721071043
1201	35	0.08545785153320651
1201	36	0.1612562917930589
1201	37	0.2531965659138109
1201	38	0.21046772584342222
1201	39	1.3976429481020887
1201	40	0.23939795975822875
1201	41	0.2768762214171474
1201	42	0.3915513704531435
1201	43	0.013771581680863676
1201	44	0.05133948226844609
1201	45	0.3271965724034729
1201	46	0.2836051108012114
1201	47	0.12009539953553627
1201	48	0.4386007976900359
1201	49	0.0899252927703671
1201	50	0.31878462215719594
1201	51	0.13996146962417555
1201	52	0.17296129124135717
1201	53	0.02079622123331859
1201	54	-0.011252848892233658
1201	55	0.17691323133861658
1201	56	0.11475594637889941
1201	57	0.10199120668192307
1201	58	0.2532610633395578
1201	59	-0.28934530062139885
1201	60	-0.9440688643365647
1201	61	-0.5424792067687818
1202	1	0.18134802653040083
1202	2	-0.03471813801149182
1202	3	0.10719453096850629
1202	4	-0.3580859585595988
1202	5	0.08961550379940064
1202	6	0.18082010330401488
1202	7	0.060612745290811176
1202	8	0.07714503489300739
1202	9	0.08370883773999793
1202	10	0.02940642873451793
1202	11	-0.004006807655834166
1202	12	0.20576777125575063
1202	13	-0.3208347684278081
1202	14	-0.04129710794703101
1202	15	0.04599924122335608
1202	16	0.1425719296027026
1202	17	-0.04468189290388125
1202	18	-0.0430204061320012
1202	19	-0.10695638018471243
1202	20	-0.3218113817272297
1202	21	-0.022685314403631196
1202	22	-0.23392441022798494
1202	23	-0.027819608982284194
1202	24	0.07823579769654998
1202	25	0.06983250569722799
1202	26	0.17167070708254073
1202	27	0.32489910747993633
1202	28	0.2842276660336225
1202	29	0.07035816210018453
1202	30	0.10314830755544335
1202	31	0.12580360972097537
1202	32	-0.023867536763049202
1202	33	0.03134350887027182
1202	34	0.21512578428824725
1202	35	0.24507825411770057
1202	36	0.009774557200614709
1202	37	-0.18748745496093733
1202	38	-0.21303811411681295
1202	39	1.0143023665911528
1202	40	0.2464028056826848
1202	41	-0.27861893265839655
1202	42	-0.09085108978542422
1202	43	-0.2571187016193335
1202	44	0.3638705458867335
1202	45	-0.041311131373859666
1202	46	0.4107485004632423
1202	47	0.1638019104699282
1202	48	-0.017987795749327518
1202	49	0.17283408998707017
1202	50	-0.29655459255453565
1202	51	0.18576477479316367
1202	52	0.24443867692723842
1202	53	0.15395042103450862
1202	54	-0.04354348010495812
1202	55	-0.08961261359180384
1202	56	-0.5977657981539011
1202	57	-0.13896928039561374
1202	58	0.45860711204731075
1202	59	-0.1221453751740853
1202	60	0.7226185114089603
1202	61	0.2396405745433441
1203	1	0.2935032203673771
1203	2	0.37123675761475283
1203	3	0.552351712877055
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2403	22	-0.12939224648529546
2403	23	0.13076043487736655
2403	24	-0.19360265259584963
2403	25	-0.09302689976086498
2403	26	-0.13157003365819975
2403	27	-0.3215738457364381
2403	28	-0.026810345662283908
2403	29	-0.08442984959572186
2403	30	-0.22308829088510151
2403	31	-0.1927270920334081
2403	32	-0.1706146127864443
2403	33	-0.22444352816676627
2403	34	-0.05630148179362493
2403	35	-0.1906399476366829
2403	36	-0.12888589601575973
2403	37	-0.2554242239667843
2403	38	-0.17860524374643916
2403	39	1.0011292428094762
2403	40	-0.46151934344012346
2403	41	-0.16673114026073677
2403	42	-0.35393283929809627
2403	43	-0.1844000125355123
2403	44	-0.6018641032360748
2403	45	-0.5978108877091444
2403	46	-0.5223350765348087
2403	47	0.010019015733085723
2403	48	-0.1872890871723314
2403	49	-0.1837387657634153
2403	50	-0.5039437251081438
2403	51	-0.17831600006064008
2403	52	0.02090675684926424
2403	53	-0.09231846298108337
2403	54	-0.5460867305922861
2403	55	-0.3577656350342977
2403	56	-0.045545403417058594
2403	57	-0.2804471069063368
2403	58	-0.29095124565249364
2403	59	0.6766637135198721
2403	60	0.14092991491773432
2403	61	0.3413096778571827
2404	1	0.15342893738311147
2404	2	-0.10551513413552271
2404	3	-0.13390285275242064
2404	4	0.2581325491923394
2404	5	-0.053431976820753846
2404	6	-0.06112514863396967
2404	7	-0.08885430897275626
2404	8	-0.14154440309148697
2404	9	-0.0432897087716313
2404	10	-0.25178300924997643
2404	11	0.039355595444938274
2404	12	0.046186860403039987
2404	13	0.16172337110707247
2404	14	-0.07472734050517005
2404	15	0.0421232722311089
2404	16	0.10199537921510426
2404	17	-0.04062423786512426
2404	18	-0.0924995727986655
2404	19	-0.043305701890140824
2404	20	0.2455335795130793
2404	21	-0.10383841517882786
2404	22	0.01898256651620045
2404	23	0.37800645690919055
2404	24	0.25301777444073537
2404	25	-0.13595722298494195
2404	26	0.16731024196626265
2404	27	0.18815007647218351
2404	28	0.16358813114990056
2404	29	-0.13434145030291944
2404	30	0.08485857887327342
2404	31	0.0683908190232998
2404	32	0.18325455626020926
2404	33	0.029647772436163677
2404	34	-0.0038059017582625643
2404	35	0.21001284714095902
2404	36	-0.06102243892341619
2404	37	0.2942833783723984
2404	38	0.0866833006118668
2404	39	1.2719937425601415
2404	40	0.020324801806715698
2404	41	0.47640771462842224
2404	42	-0.0362847138164355
2404	43	0.08632848830314543
2404	44	-0.21304321380318925
2404	45	0.5828410004090827
2404	46	0.18073396557952037
2404	47	0.0638745848885307
2404	48	-0.009085179470254445
2404	49	0.15018389618862216
2404	50	-0.03601680185686007
2404	51	0.016978437583858863
2404	52	0.3942691032838255
2404	53	0.1358423783988485
2404	54	-0.11179687157782325
2404	55	0.3633912623771849
2404	56	0.6215292599856355
2404	57	0.16662664983694242
2404	58	0.7491231004194034
2404	59	1.1785450318026562
2404	60	1.2388491315640024
2404	61	1.892014605551097
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	10.0
18	18.0
19	38.0
20	52.0
21	67.0
22	97.0
23	144.0
24	243.0
25	311.0
26	446.0
27	571.0
28	774.0
29	851.0
30	1085.0
31	1270.0
32	1587.0
33	1823.0
34	2218.0
35	2815.0
36	4555.0
37	7502.0
38	27907.0
39	80099.0
40	27526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.116659465465094	56.29158693907783	23.86272452317758	9.72902907227949
2	56.8260966212211	10.866800039517882	10.721077850227227	21.586025489033787
3	10.349361150546263	57.191531386951425	22.176408863650394	10.282698598851923
4	10.614225135640613	11.063582887370455	56.87029732916072	21.451894647828208
5	11.214739830874636	10.61477686562558	21.93136226158879	56.23912104191099
6	57.617431022776366	11.595580519721004	10.249367323004753	20.537621134497872
7	57.682859082772666	11.243133139929634	21.562249243873836	9.511758533423862
8	8.600086414418863	63.946669958644534	7.06437874205296	20.38886488488365
9	15.496574285537928	56.830442565273756	20.258626010740077	7.414357138448244
10	54.49601876427381	17.098944509598173	20.53330041355472	7.871736312573298
11	9.059317326090982	54.97747052651071	27.58718597617431	8.376026171224
12	54.947225479908646	10.04135547188445	19.827171162273935	15.184247885932967
13	16.476760693784335	9.935806431701746	6.785383618295167	66.80204925621875
14	16.269366088513053	56.798345781124624	6.826739090179619	20.105549040182705
15	9.337077958150731	17.560644404666377	19.49324115795321	53.60903647922968
16	9.758656873032528	62.75847169927782	7.377322387506944	20.105549040182705
17	15.250293191778287	11.739398802543052	7.826677365594717	65.18363064008395
18	55.29782112215295	16.560706129251283	19.41485093512746	8.726621813468306
19	14.687982223319548	9.968520461699896	53.39546941546818	21.948027899512375
20	11.861613480649343	10.252453552249861	8.38405036726128	69.50188259983952
21	13.634343559039566	10.647490895623726	65.84470094438615	9.873464600950559
22	56.15887908153818	11.088821677674218	10.681439417319918	22.070859823467686
23	11.646811925189802	56.547126720572805	8.95870625270045	22.84735510153694
24	24.224430590704277	57.020554286772416	8.904388617986545	9.850626504536757
25	12.508487130424047	12.377013764582433	21.207950126535398	53.906548978458126
26	11.435096598975372	13.596691562249244	65.48855008950065	9.479661749274735
27	23.863341769026604	12.828837726066292	8.472316523671378	54.835503981235725
28	12.270847478550708	13.399790136411333	19.54015184247886	54.7892105425591
29	12.296771804209616	57.47793346089748	19.596938460588852	10.628356274304055
30	67.24955249675946	14.733041170298131	8.069872230109253	9.947534102833158
31	10.209246342818345	15.19597555706438	64.22196160730819	10.372816492809084
32	10.864761434479353	13.81396210110487	20.224677489043884	55.09659897537189
33	22.230109252515277	57.7322387506944	9.175359545707055	10.862292451083267
34	10.291340040738225	13.164619467934077	20.654280599962966	55.88975989136473
35	11.054255910129005	11.87951361027097	20.47527930374668	56.59095117585334
36	20.97463119560521	13.978766742793654	9.521634467008209	55.52496759459292
37	11.648663662736867	68.48280970310473	8.26924263934325	11.599283994815135
38	21.38263070180853	14.379976544657739	9.048824146657614	55.18856860687612
39	11.598949836867863	68.38550163132136	8.756244902120718	11.259303629690049
40	20.42651688167397	14.146657613727548	53.27202024566384	12.154805258934633
41	10.978951916548361	23.844207147706932	53.80285167582248	11.373989259922226
42	65.10770940065427	14.45898401333251	8.257514968211838	12.175791617801371
43	9.427813097956916	68.73155978026048	9.245725572495525	12.59490154928708
44	9.387074871921486	14.654033701623357	63.27140299981483	12.68748842664033
45	9.939509906795877	22.920190111721496	9.29942596136041	57.840874020122214
46	19.17350780815999	14.175050922782544	53.47262514659589	13.178816122461576
47	53.899142028269864	22.722671440034567	9.213628788346398	14.164557743349176
48	53.446700820936975	13.086229245108328	19.237084130609222	14.229985803345471
49	54.39972841182643	12.888710573421394	8.835257082896117	23.876303931856057
50	64.88550089500647	12.585025615702735	8.653786803283747	13.875686686007036
51	63.72446145299673	13.059070427751374	9.658663045491018	13.557805073760878
52	54.11209184618233	23.697919881488797	8.885253996666872	13.304734275661998
53	54.80525893463366	22.239367940250602	9.89630269736436	13.059070427751374
54	64.62502314671934	14.026294673168325	9.48892043701006	11.859761743102277
55	54.97253255971854	22.992407876057033	9.587062527004505	12.447997037219926
56	64.73304117029814	12.95845935436084	9.796926115671873	12.511573359669157
57	54.78674155916301	13.462131967162522	9.163014628726621	22.588111844947843
58	55.347200790074695	22.092463428183446	9.614221344361459	12.94611443738041
59	55.56817480402444	12.676378001357941	9.697549533979384	22.057897660638233
60	55.9656811307944	21.550521572742422	9.382136905129313	13.10166039133387
61	57.77914943522005	10.561076476760693	9.703721992469601	21.95605209554966
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	0.5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	2.0
12	2.5
13	2.0
14	3.0
15	4.0
16	6.0
17	9.5
18	11.0
19	16.5
20	31.5
21	41.0
22	61.5
23	82.0
24	96.0
25	141.5
26	173.0
27	159.0
28	145.0
29	267.0
30	474.0
31	559.0
32	585.5
33	612.0
34	814.0
35	1139.5
36	1263.0
37	1408.5
38	1554.0
39	1570.0
40	1734.5
41	1883.0
42	2041.0
43	2383.5
44	2568.0
45	3358.0
46	4148.0
47	12590.0
48	14942.5
49	8853.0
50	9171.5
51	9490.0
52	9258.0
53	8064.0
54	7102.0
55	6077.5
56	5053.0
57	4259.0
58	3260.0
59	3055.0
60	4314.5
61	36321.0
62	67068.0
63	34510.5
64	1953.0
65	1271.0
66	482.0
67	375.0
68	343.5
69	312.0
70	263.5
71	190.0
72	165.0
73	138.5
74	112.0
75	101.0
76	61.5
77	33.0
78	26.0
79	19.0
80	18.5
81	14.0
82	10.0
83	8.0
84	5.5
85	5.0
86	3.0
87	1.0
88	0.5
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.5
95	1.0
96	2.5
97	4.0
98	2.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03580025924325659
3	0.0
4	6.172458490216652E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1368434047281033
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	162010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.003333127584717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95461450816559	20.53083143015863
2	12.97711735662742	6.748966113202888
3	3.3232054690467145	2.5924325658909946
4	1.172616786935055	1.2196777976668107
5	0.6242878845423471	0.8116782914634899
6	0.42014812001519175	0.6555150916610085
7	0.2800987466767946	0.5098450712918956
8	0.17565514622104064	0.36540954262082587
9	0.14717052791492596	0.34442318375408926
>10	1.6378655526015953	9.142028269859885
>50	0.189897455374098	3.3572001728288376
>100	0.08782757311052032	4.0213567063761495
>500	0.002373718192176225	0.3950373433738658
>1k	0.002373718192176225	1.2789333991728906
>5k	0.0	0.0
>10k+	0.00474743638435245	48.02666502067773
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGGG	63170	38.9914202826986	TruSeq Adapter, Index 23 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC	14638	9.035244737979136	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACNTTGATCTGGGGGGGGGGGGGGG	2072	1.2789333991728906	TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGGT	640	0.3950373433738658	TruSeq Adapter, Index 23 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGNGAGATAGATCGGAAGAGCACAC	486	0.29998148262452934	No Hit
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATCGGAAGAGCACACGTCT	452	0.27899512375779273	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGTG	400	0.24689833960866614	TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACGTTGATCTGGGGGGGGGGGGGGG	348	0.21480155545953952	TruSeq Adapter, Index 23 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTAGAGAGATAGATCGGAAGAGCACACG	305	0.18825998395160792	No Hit
AGATCGGAAGAACACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGGG	258	0.15924942904758965	TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGAAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGGG	219	0.1351768409357447	TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCT	215	0.13270785753965805	TruSeq Adapter, Index 23 (97% over 37bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGG	203	0.12530090735139804	TruSeq Adapter, Index 23 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAAGAGATAGATCGGAAGAGCACACG	196	0.12098018640824641	No Hit
AGATCGGAAGAGCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGAT	172	0.10616628603172644	TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGG	172	0.10616628603172644	TruSeq Adapter, Index 23 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	44.964508363681254	0.0	0.0	0.0	0.0
2	45.193506573668294	0.0	0.0	0.0	0.0
3	45.33732485649034	0.0	0.0	0.0	0.0
4	45.4786741559163	0.0	0.0	0.0	0.0
5	45.69038948213073	0.0	0.0	0.0	0.0
6	46.03234368248874	0.0	0.0	0.0	0.0
7	46.57428553792976	0.0	0.0	0.0	0.0
8	51.409172273316464	0.0	0.0	0.0	0.0
9	51.97395222517129	0.0	0.0	0.0	0.0
10	52.37207579779026	0.0	0.0	0.0	0.0
11	52.626998333436205	0.0	0.0	0.0	0.0
12	53.12449848774767	0.0	0.0	0.0	0.0
13	53.623850379606196	0.0	0.0	0.0	0.0
14	54.186161348064935	0.0	0.0	0.0	0.0
15	54.9145114499105	0.0	0.0	0.0	0.0
16	55.76939695080551	0.0	0.0	0.0	0.0
17	56.30948706869946	0.0	0.0	0.0	0.0
18	56.62736868094562	0.0	0.0	0.0	0.0
19	57.179803715820015	0.0	0.0	0.0	0.0
20	57.40633294241096	0.0	0.0	0.0	0.0
21	57.665576199000064	0.0	0.0	0.0	0.0
22	57.91432627615579	0.0	0.0	0.0	0.0
23	58.17356953274489	0.0	0.0	0.0	0.0
24	58.44021973952225	0.0	0.0	0.0	0.0
25	58.696994012715265	0.0	0.0	0.0	0.0
26	58.98401333251034	0.0	0.0	0.0	0.0
27	59.24387383494846	0.0	0.0	0.0	0.0
28	59.53768285908277	0.0	0.0	0.0	0.0
29	59.809888278501326	0.0	0.0	0.0	0.0
30	60.14875624961422	0.0	0.0	0.0	0.0
31	60.553669526572435	0.0	0.0	0.0	0.0
32	61.00796247145238	0.0	0.0	0.0	0.0
33	61.54496636010123	0.0	0.0	0.0	0.0
34	62.00975248441454	0.0	0.0	0.0	0.0
35	62.41466576137275	0.0	0.0	0.0	0.0
36	62.83007221776433	0.0	0.0	0.0	0.0
37	63.2936238503796	0.0	0.0	0.0	0.0
38	63.60656749583359	0.0	0.0	0.0	0.0
39	63.960249367323	0.0	0.0	0.0	0.0
40	64.30837602617122	0.0	0.0	0.0	0.0
41	65.02808468613048	0.0	0.0	0.0	0.0
42	65.52249861119684	0.0	0.0	0.0	0.0
43	65.97370532683168	0.0	0.0	0.0	0.0
44	67.20079007468675	0.0	0.0	0.0	0.0
45	77.6155792852293	0.0	0.0	0.0	0.0
46	78.14702796123696	0.0	0.0	0.0	0.0
47	78.52231343744214	0.0	0.0	0.0	0.0
48	78.95808900685142	0.0	0.0	0.0	0.0
49	79.36979198814888	0.0	0.0	0.0	0.0
50	79.8605024381211	0.0	0.0	0.0	0.0
>>END_MODULE