FastQCFastQC Report
Sat 10 Aug 2024
AM-AL-EC05_S55_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-EC05_S55_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162010
Sequences flagged as poor quality0
Sequence length61
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGGG6317038.9914202826986TruSeq Adapter, Index 23 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC146389.035244737979136No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACNTTGATCTGGGGGGGGGGGGGGG20721.2789333991728906TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGGT6400.3950373433738658TruSeq Adapter, Index 23 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGNGAGATAGATCGGAAGAGCACAC4860.29998148262452934No Hit
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATCGGAAGAGCACACGTCT4520.27899512375779273Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGTG4000.24689833960866614TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACGTTGATCTGGGGGGGGGGGGGGG3480.21480155545953952TruSeq Adapter, Index 23 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTAGAGAGATAGATCGGAAGAGCACACG3050.18825998395160792No Hit
AGATCGGAAGAACACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGGG2580.15924942904758965TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGAAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGGG2190.1351768409357447TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCT2150.13270785753965805TruSeq Adapter, Index 23 (97% over 37bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGGGGGGGGGGGGG2030.12530090735139804TruSeq Adapter, Index 23 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAAGAGATAGATCGGAAGAGCACACG1960.12098018640824641No Hit
AGATCGGAAGAGCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGAT1720.10616628603172644TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACATTGATCTGGG1720.10616628603172644TruSeq Adapter, Index 23 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)