##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-EC05.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16691 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.18662752381523 40.0 40.0 40.0 40.0 40.0 2 38.90264214247199 40.0 40.0 40.0 40.0 40.0 3 38.99826253669642 40.0 40.0 40.0 40.0 40.0 4 38.80558384758253 40.0 40.0 40.0 40.0 40.0 5 38.90096459169612 40.0 40.0 40.0 40.0 40.0 6 39.03540830387634 40.0 40.0 40.0 40.0 40.0 7 39.18566892337188 40.0 40.0 40.0 40.0 40.0 8 39.06368701695524 40.0 40.0 40.0 40.0 40.0 9 39.24677970163561 40.0 40.0 40.0 40.0 40.0 10-11 39.20244443113055 40.0 40.0 40.0 40.0 40.0 12-13 39.20124618057636 40.0 40.0 40.0 40.0 40.0 14-15 39.20771673356899 40.0 40.0 40.0 40.0 40.0 16-17 39.31615840872326 40.0 40.0 40.0 40.0 40.0 18-19 39.32131088610628 40.0 40.0 40.0 40.0 40.0 20-21 39.235755796537056 40.0 40.0 40.0 40.0 40.0 22-23 39.29590797435744 40.0 40.0 40.0 40.0 40.0 24-25 39.30153975196214 40.0 40.0 40.0 40.0 40.0 26-27 39.011203642681686 40.0 40.0 40.0 40.0 40.0 28-29 38.99826253669642 40.0 40.0 40.0 40.0 40.0 30-31 39.09461996669901 40.0 40.0 40.0 40.0 40.0 32-33 39.14411998026705 40.0 40.0 40.0 40.0 40.0 34-35 39.14982727916407 40.0 40.0 40.0 40.0 40.0 36-37 39.11943841120286 40.0 40.0 40.0 40.0 40.0 38-39 39.06974830153355 40.0 40.0 40.0 40.0 40.0 40-41 39.12786086751126 40.0 40.0 40.0 40.0 40.0 42-43 39.05801723936744 40.0 40.0 40.0 40.0 40.0 44-45 38.9860894407925 40.0 40.0 40.0 40.0 40.0 46-47 38.91017554367453 40.0 40.0 40.0 40.0 40.0 48-49 38.93765776549239 40.0 40.0 40.0 40.0 40.0 50-51 38.905201170233 40.0 40.0 40.0 40.0 40.0 52-53 38.83622169810894 40.0 40.0 40.0 40.0 40.0 54-55 38.69904513377641 40.0 40.0 40.0 40.0 40.0 56-57 38.714012585116436 40.0 40.0 40.0 40.0 40.0 58-59 38.573713262239686 40.0 40.0 40.0 40.0 40.0 60-61 38.45108408216001 40.0 40.0 40.0 40.0 40.0 62-63 39.13039148285319 40.0 40.0 40.0 40.0 40.0 64-65 39.06061703678337 40.0 40.0 40.0 40.0 40.0 66-67 39.021647220074506 40.0 40.0 40.0 40.0 40.0 68-69 39.0423585017226 40.0 40.0 40.0 40.0 40.0 70-71 39.058984576834305 40.0 40.0 40.0 40.0 40.0 72-73 38.97966709110085 40.0 40.0 40.0 40.0 40.0 74-75 39.05322435484642 40.0 40.0 40.0 40.0 40.0 76-77 39.05916066406101 40.0 40.0 40.0 40.0 40.0 78-79 39.0032422417786 40.0 40.0 40.0 40.0 40.0 80-81 38.969763567696205 40.0 40.0 40.0 40.0 40.0 82-83 38.9357693696756 40.0 40.0 40.0 40.0 40.0 84-85 38.80738023656688 40.0 40.0 40.0 40.0 40.0 86-87 38.890777522954764 40.0 40.0 40.0 40.0 40.0 88-89 39.11578729567556 40.0 40.0 40.0 40.0 40.0 90-91 39.33505154639175 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.120643055507756 1101 2 -0.7810062873869228 1101 3 -0.32616976100447204 1101 4 -1.692351253944139 1101 5 -0.10038601932602376 1101 6 0.22354639055688352 1101 7 -2.9060592244742764 1101 8 -1.3031525018501071 1101 9 -0.31691761163330057 1101 10-11 -0.565611039354394 1101 12-13 -0.0757245955198087 1101 14-15 -0.6441728333269126 1101 16-17 0.1393668866887623 1101 18-19 -0.4615591365155822 1101 20-21 -0.3919230842263275 1101 22-23 -0.5191193815625397 1101 24-25 -0.6912195779191492 1101 26-27 -0.29235088254502983 1101 28-29 -0.12538848604164343 1101 30-31 0.0681778889183633 1101 32-33 -0.15530358755622586 1101 34-35 -0.16274240622789904 1101 36-37 0.3028053490546654 1101 38-39 0.20564743870004776 1101 40-41 -1.3949785990850145 1101 42-43 -0.2517722699131184 1101 44-45 0.8915978269665246 1101 46-47 0.5716074910453486 1101 48-49 -1.2269384628190068 1101 50-51 0.32151823588451833 1101 52-53 0.5502303055401256 1101 54-55 -0.24468361692511564 1101 56-57 1.553899578247659 1101 58-59 0.2936768280988318 1101 60-61 1.2835943209722984 1101 62-63 0.7919123564344162 1101 64-65 -1.4848955050881116 1101 66-67 1.1087187574851995 1101 68-69 0.03456335331335225 1101 70-71 -0.08032054750805173 1101 72-73 1.292190795315797 1101 74-75 1.006218954656454 1101 76-77 0.6805059523809547 1101 78-79 0.8839285714285694 1101 80-81 2.4791666666666714 1101 82-83 2.514880952380956 1101 84-85 4.591666666666669 1101 86-87 11.489583333333332 1101 88-89 10.416666666666664 1101 90-91 21.75 1102 1 -0.7303991530687313 1102 2 -5.184825088733191E-4 1102 3 -0.618852687833737 1102 4 -1.1069854002856019 1102 5 -0.39306894615528876 1102 6 0.6137902929959083 1102 7 0.6061358974769462 1102 8 0.5505060347352568 1102 9 -0.024234684804035567 1102 10-11 0.5563401801577967 1102 12-13 0.31451930691921604 1102 14-15 0.4289978983804019 1102 16-17 0.529610789127787 1102 18-19 0.27014818055759093 1102 20-21 -0.1480206452019317 1102 22-23 0.21258793551063349 1102 24-25 0.04048773915401682 1102 26-27 0.0978930198939949 1102 28-29 -0.07660799823676001 1102 30-31 -1.541578208642612 1102 32-33 0.7677733355206939 1102 34-35 -0.06828741993231091 1102 36-37 -0.10620653155964987 1102 38-39 -1.105508475278441 1102 40-41 -0.683566448017686 1102 42-43 -0.39817031631116606 1102 44-45 -1.5299406345719362 1102 46-47 -1.5236306041927534 1102 48-49 -1.3534785893591348 1102 50-51 -2.0416396588523256 1102 52-53 -2.0811422434794906 1102 54-55 -1.3505659698662882 1102 56-57 -3.071100421752341 1102 58-59 -2.49362475920276 1102 60-61 0.712165749543729 1102 62-63 0.22048378500584676 1102 64-65 0.598437828245217 1102 66-67 -0.29128124251479903 1102 68-69 1.177420496170491 1102 70-71 0.17364770646020133 1102 72-73 -1.0411425380175316 1102 74-75 -2.493781045343546 1102 76-77 -2.8194940476190453 1102 78-79 -1.1160714285714306 1102 80-81 2.4791666666666714 1102 82-83 2.514880952380956 1102 84-85 4.591666666666669 1102 86-87 11.489583333333332 1102 88-89 13.083333333333336 1102 90-91 21.75 1103 1 0.030576456687370523 1103 2 0.47021322480819805 1103 3 0.03480584875162407 1103 4 0.834478014348548 1103 5 1.0703456879910505 1103 6 0.6040341954349344 1103 7 0.2061358974769476 1103 8 0.1407499371742844 1103 9 -0.04374687992598325 1103 10-11 0.7514621313773091 1103 12-13 0.15476320935824361 1103 14-15 -0.7307581991805705 1103 16-17 0.7247327403472994 1103 18-19 0.06039208299661425 1103 20-21 0.1788086230907524 1103 22-23 0.4028318379496554 1103 24-25 0.22585359281255535 1103 26-27 0.07960033696716806 1103 28-29 -0.2497787299440759 1103 30-31 -0.25133430620358865 1103 32-33 -0.260798093050731 1103 34-35 -0.3329933022852529 1103 36-37 0.8643817037344661 1103 38-39 -0.46101922796660944 1103 40-41 0.08782563371921981 1103 42-43 0.6183132001723521 1103 44-45 -0.12289492665666302 1103 46-47 -1.248268285352168 1103 48-49 -0.4010976369781787 1103 50-51 0.07389918826546449 1103 52-53 0.5502303055401256 1103 54-55 0.8847281477807698 1103 56-57 1.553899578247659 1103 58-59 0.6825657169877175 1103 60-61 0.750260987638967 1103 62-63 -0.9003953358732772 1103 64-65 -0.016946787139396235 1103 66-67 1.1087187574851995 1103 68-69 0.2885316072816053 1103 70-71 1.062536595349087 1103 72-73 1.292190795315797 1103 74-75 -0.13663818820068485 1103 76-77 -0.6528273809523739 1103 78-79 0.8839285714285694 1103 80-81 2.4791666666666714 1103 82-83 2.514880952380956 1103 84-85 4.591666666666669 1103 86-87 11.489583333333332 1103 88-89 13.083333333333336 1103 90-91 -18.25 1104 1 0.19421282032373455 1104 2 -0.8297867751917991 1104 3 -2.4924668785210997 1104 4 -0.038249258378726836 1104 5 -0.3841997665544028 1104 6 -0.0868748954741605 1104 7 -0.4847731934321473 1104 8 -0.4228864264620853 1104 9 0.02898039280128728 1104 10-11 -0.8394469595317844 1104 12-13 -0.02250951791449296 1104 14-15 0.09196907354669293 1104 16-17 -1.3207218051072473 1104 18-19 -0.06688064427611806 1104 20-21 -0.31210046781834677 1104 22-23 -0.6244408893230684 1104 24-25 -0.3741464071874461 1104 26-27 -0.0340360266691917 1104 28-29 0.16840308823773142 1104 30-31 0.3370377868196712 1104 32-33 -0.02086145311409382 1104 34-35 0.03929277689738342 1104 36-37 0.8643817037344661 1104 38-39 -0.157988924936312 1104 40-41 0.9026404485340365 1104 42-43 0.9260055078646587 1104 44-45 0.510645446014145 1104 46-47 -1.2780165691050271 1104 48-49 -0.6550658909464318 1104 50-51 -0.41181509744881595 1104 52-53 0.24546840077821486 1104 54-55 0.7838878116463164 1104 56-57 -1.3691773448292608 1104 58-59 -0.04820351378150889 1104 60-61 1.2835943209722984 1104 62-63 0.7919123564344162 1104 64-65 1.2651044949118884 1104 66-67 1.1087187574851995 1104 68-69 1.177420496170491 1104 70-71 1.062536595349087 1104 72-73 1.292190795315797 1104 74-75 1.006218954656454 1104 76-77 -1.3194940476190453 1104 78-79 0.8839285714285694 1104 80-81 2.4791666666666714 1104 82-83 2.514880952380956 1104 84-85 4.591666666666669 1104 86-87 11.489583333333332 1104 88-89 13.083333333333336 1104 90-91 21.75 1201 1 0.8305764566873677 1201 2 0.8146576692526466 1201 3 -0.09852748458170879 1201 4 0.8789224587929922 1201 5 0.35923457687994187 1201 6 -0.06263247123173699 1201 7 0.6061358974769462 1201 8 0.9407499371742816 1201 9 0.04514200896290532 1201 10-11 -0.2263156464004652 1201 12-13 0.038096542691576474 1201 14-15 -0.42520264362502047 1201 16-17 0.5469549625695223 1201 18-19 -0.006274583670048628 1201 20-21 -0.06563582135369472 1201 22-23 0.6028318379496582 1201 24-25 0.6258535928125539 1201 26-27 0.2962670036338366 1201 28-29 0.4946657145003641 1201 30-31 0.4931101382408585 1201 32-33 0.20055836104536695 1201 34-35 -0.39392265268828197 1201 36-37 0.05261699785211249 1201 38-39 0.14504137809399253 1201 40-41 0.6267783795685204 1201 42-43 0.38754396940311864 1201 44-45 -0.011735506366811421 1201 46-47 1.143036062473918 1201 48-49 1.1227118868313397 1201 50-51 0.7004656043055704 1201 52-53 -0.22871706288093208 1201 54-55 0.9342637514959407 1201 56-57 1.053899578247659 1201 58-59 0.6825657169877175 1201 60-61 -0.24973901236103302 1201 62-63 -1.1474815829595215 1201 64-65 -2.007622777815385 1201 66-67 -1.3357256869592433 1201 68-69 -0.15591283716283755 1201 70-71 1.062536595349087 1201 72-73 -2.3744758713508674 1201 74-75 -4.127114378676879 1201 76-77 -3.719494047619051 1201 78-79 -4.716071428571432 1201 80-81 -3.12083333333333 1201 82-83 0.9148809523809547 1201 84-85 2.991666666666667 1201 86-87 4.822916666666668 1201 88-89 13.083333333333336 1201 90-91 21.75 1202 1 -0.44601928799348656 1202 2 -0.787233583702438 1202 3 0.5539547849218422 1202 4 0.213201418603866 1202 5 0.049069092246369905 1202 6 0.06786398266897464 1202 7 0.2657103655620503 1202 8 -0.42095219048529486 1202 9 -0.17991709269194445 1202 10-11 -0.057048506920565956 1202 12-13 0.5345504434007964 1202 14-15 0.010731162521551596 1202 16-17 -0.46675662135482554 1202 18-19 -0.5310972787055093 1202 20-21 -0.07225520669648944 1202 22-23 -0.03546603439077245 1202 24-25 -0.18265704548532113 1202 26-27 -0.23422945026687358 1202 28-29 -0.42637447462493583 1202 30-31 -0.42793005088443437 1202 32-33 -0.6159475947118622 1202 34-35 0.4336733643814199 1202 36-37 -0.8320129831725538 1202 38-39 0.072314105366722 1202 40-41 0.6169261628197518 1202 42-43 0.6060055078646585 1202 44-45 0.558264493633196 1202 46-47 -0.5712496518117973 1202 48-49 1.1227118868313397 1202 50-51 -0.2118150974488131 1202 52-53 0.8796420702460068 1202 54-55 0.8553163830748858 1202 56-57 -0.01752899318091039 1202 58-59 -0.6635881291661292 1202 60-61 -1.0240979867200082 1202 62-63 -1.7686937041716462 1202 64-65 -7.234895505088112 1202 66-67 -4.3912812425148005 1202 68-69 -0.822579503829509 1202 70-71 -1.73746340465091 1202 72-73 -3.1078092046842087 1202 74-75 -0.5937810453435475 1202 76-77 0.6805059523809547 1202 78-79 0.8839285714285694 1202 80-81 2.4791666666666714 1202 82-83 2.514880952380956 1202 84-85 4.591666666666669 1202 86-87 11.489583333333332 1202 88-89 13.083333333333336 1202 90-91 21.75 1203 1 0.1194653455762591 1203 2 0.5479910025859809 1203 3 -0.09852748458170879 1203 4 -0.18774420787367774 1203 5 0.7147901324354962 1203 6 -0.06263247123173699 1203 7 0.2505803419213919 1203 8 0.9407499371742816 1203 9 -0.2215246577037604 1203 10-11 0.26257324248842195 1203 12-13 -0.006347901752867813 1203 14-15 0.46368624526387237 1203 16-17 -0.3419339263193635 1203 18-19 -0.050719028114492914 1203 20-21 -0.11008026579813901 1203 22-23 -0.9527237176058989 1203 24-25 -0.21859085163188752 1203 26-27 0.16293367030050376 1203 28-29 0.4946657145003641 1203 30-31 0.004221249351964218 1203 32-33 0.2561454285439524 1203 34-35 0.09683125911826096 1203 36-37 -0.959147708030244 1203 38-39 -0.5394506005156359 1203 40-41 -0.6961498740466041 1203 42-43 -0.21831122265896852 1203 44-45 -0.3760944807257829 1203 46-47 -1.1033407491202851 1203 48-49 -0.20930392344534 1203 50-51 -1.9160757490778906 1203 52-53 -1.281348641828302 1203 54-55 -2.5394204590303744 1203 56-57 -1.9127670884190096 1203 58-59 -0.4994855650635657 1203 60-61 1.2835943209722984 1203 62-63 -5.874754310232255 1203 64-65 -3.6237843939769974 1203 66-67 -3.780170131403686 1203 68-69 -2.6003572816072875 1203 70-71 -1.2707967379842415 1203 72-73 -0.04114253801753165 1203 74-75 1.006218954656454 1203 76-77 0.6805059523809547 1203 78-79 0.8839285714285694 1203 80-81 0.1458333333333428 1203 82-83 -0.1517857142857153 1203 84-85 1.9249999999999972 1203 86-87 11.489583333333332 1203 88-89 9.083333333333336 1203 90-91 21.75 1204 1 0.8305764566873677 1204 2 -0.31815886821505757 1204 3 -0.1605429884576779 1204 4 0.4902919678369173 1204 5 -0.697096172474069 1204 6 -1.3215471999139012 1204 7 -0.04502689322072939 1204 8 0.19656389066265234 1204 9 -0.36002594969342994 1204 10-11 0.23983422440056756 1204 12-13 -0.22546934878129576 1204 14-15 0.12156738221477781 1204 16-17 0.3526397170914848 1204 18-19 -0.5489102425847818 1204 20-21 -0.47700533039762405 1204 22-23 0.2307388146938436 1204 24-25 -0.3043789653269826 1204 26-27 0.27146080208344614 1204 28-29 0.4781282468001038 1204 30-31 0.15099127519176392 1204 32-33 -0.04352319849727593 1204 34-35 -0.29110381921929473 1204 36-37 -1.0969086188461787 1204 38-39 0.08659981965243446 1204 40-41 -0.9306928847992921 1204 42-43 -1.7406611588020127 1204 44-45 -0.9179259825572856 1204 46-47 -0.43791631847846446 1204 48-49 -0.9825512710634001 1204 50-51 0.6770737914400726 1204 52-53 -0.832776230407589 1204 54-55 0.4141399124866538 1204 56-57 1.053899578247659 1204 58-59 -0.3174342830122825 1204 60-61 -0.7164056790277016 1204 62-63 0.7919123564344162 1204 64-65 0.4651044949118912 1204 66-67 0.10871875748519955 1204 68-69 -0.822579503829509 1204 70-71 0.06253659534908707 1204 72-73 -0.04114253801753165 1204 74-75 -0.5937810453435475 1204 76-77 0.6805059523809547 1204 78-79 -1.782738095238102 1204 80-81 -1.5208333333333286 1204 82-83 2.514880952380956 1204 84-85 4.591666666666669 1204 86-87 -28.510416666666668 1204 88-89 -26.916666666666664 1204 90-91 -18.25 1301 1 0.14972539285758302 1301 2 -0.7021272007237158 1301 3 -0.3822154278441161 1301 4 0.2983078015825882 1301 5 -2.1636968652004356 1301 6 -0.61298708116081 1301 7 0.2657103655620503 1301 8 -1.4422287862299754 1301 9 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54-55 0.26272379048229055 2402 56-57 0.7191417434898213 2402 58-59 0.30256571698772206 2402 60-61 -0.28640567902770186 2402 62-63 0.7919123564344162 2402 64-65 -0.21584788604049265 2402 66-67 1.1087187574851995 2402 68-69 0.677420496170491 2402 70-71 1.062536595349087 2402 72-73 0.14933365245865815 2402 74-75 0.39083433927184075 2402 76-77 0.6805059523809547 2402 78-79 0.08392857142857224 2402 80-81 2.4791666666666714 2402 82-83 2.514880952380956 2402 84-85 4.591666666666669 2402 86-87 11.489583333333332 2402 88-89 13.083333333333336 2402 90-91 21.75 2403 1 -0.04613587207975911 2403 2 0.2935008960410741 2403 3 -0.6282078498785069 2403 4 -1.2860699308569323 2403 5 -0.18992828461168898 2403 6 0.5656780310513696 2403 7 0.1677797330933828 2403 8 0.06403760840715478 2403 9 -0.12045920869311288 2403 10-11 0.20351692589785841 2403 12-13 -0.11715459886094237 2403 14-15 0.2986938556139478 2403 16-17 0.3959656170596233 2403 18-19 0.030255096695249506 2403 20-21 0.06647985596745798 2403 22-23 0.3836537557578765 2403 24-25 0.40667551062077223 2403 26-27 -0.07450925207393055 2403 28-29 -0.410052702546821 2403 30-31 -0.16503293634057314 2403 32-33 0.21420190694926333 2403 34-35 -0.26740190443578626 2403 36-37 -0.2647391753864099 2403 38-39 -0.047685894633282544 2403 40-41 -0.150692884799291 2403 42-43 -0.33574610025013385 2403 44-45 -0.6193465132461071 2403 46-47 0.7219834308949729 2403 48-49 0.5116007757202254 2403 50-51 -0.6112548733591794 2403 52-53 -0.36513971343521234 2403 54-55 -1.0584767203733847 2403 56-57 -0.5446225892400278 2403 58-59 -1.2618787274567254 2403 60-61 0.40359432097230297 2403 62-63 0.030007594529656956 2403 64-65 0.8651044949118898 2403 66-67 -0.37900054076041556 2403 68-69 0.10049741924741085 2403 70-71 -1.0586755258630376 2403 72-73 -0.9078092046842059 2403 74-75 0.11733006576756821 2403 76-77 -0.4623511904761841 2403 78-79 0.8839285714285694 2403 80-81 -0.1874999999999929 2403 82-83 2.514880952380956 2403 84-85 4.591666666666669 2403 86-87 3.489583333333332 2403 88-89 13.083333333333336 2403 90-91 21.75 2404 1 -0.0045883784774645164 2404 2 -0.192424137829164 2404 3 -0.127831513885738 2404 4 0.5751373550078895 2404 5 0.5428731605185249 2404 6 0.8282100196107578 2404 7 -0.2729849816439298 2404 8 0.06162905805340557 2404 9 0.2287805926014883 2404 10-11 0.2239896039047835 2404 12-13 0.0014665060615399739 2404 14-15 0.05001103158866016 2404 16-17 -0.3961463805318246 2404 18-19 0.17687559948013387 2404 20-21 -0.10031225603012928 2404 22-23 -0.254311019193203 2404 24-25 0.29618326314222543 2404 26-27 0.12630363367046726 2404 28-29 0.1908806107152614 2404 30-31 0.14536899049971197 2404 32-33 -0.011679769513811777 2404 34-35 -0.09426781208916424 2404 36-37 -0.17784051848775562 2404 38-39 0.03898077203338346 2404 40-41 -0.28528711685832064 2404 42-43 -0.2798768450765152 2404 44-45 0.256421389067107 2404 46-47 0.18135023871912637 2404 48-49 -0.08805734393789066 2404 50-51 0.29454396791646076 2404 52-53 -0.2450874202123856 2404 54-55 0.02198304974155718 2404 56-57 -0.08246405811598123 2404 58-59 -0.027111702367122348 2404 60-61 -0.57847464454494 2404 62-63 0.5061980707201315 2404 64-65 0.5839450746220294 2404 66-67 0.72776637653282 2404 68-69 0.07742049617049673 2404 70-71 1.062536595349087 2404 72-73 1.292190795315797 2404 74-75 -0.5493366008991032 2404 76-77 0.6805059523809547 2404 78-79 0.8839285714285694 2404 80-81 2.4791666666666714 2404 82-83 2.514880952380956 2404 84-85 4.591666666666669 2404 86-87 11.489583333333332 2404 88-89 13.083333333333336 2404 90-91 21.75 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 1.0 31 28.0 32 135.0 33 230.0 34 278.0 35 421.0 36 585.0 37 848.0 38 1608.0 39 5722.0 40 6835.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.777664609669884 24.25259121682344 31.80156970822599 23.168174465280693 2 21.891292622999938 26.247977467489665 26.82926829268293 25.03146161682747 3 21.784195075190222 26.25366964232221 27.55976274639027 24.402372536097296 4 23.240069498532144 25.606614343059135 25.39692049607573 25.75639566233299 5 24.060871128152897 24.55215385537116 27.026541249775327 24.360433766700616 6 24.39039003055539 26.67305733628902 25.1512791324666 23.785273500688994 7 24.18668743634294 25.942124498232584 25.540710562578635 24.330477502845845 8 19.681265352585225 40.00958600443353 18.087592115511352 22.221556527469893 9 35.084776226709 23.38385956503505 20.657839554250792 20.873524654005152 10-11 19.01324066862381 29.458989874782816 28.784973938050445 22.742795518542927 12-13 29.52489365526332 20.795638367982743 19.08813132826074 30.5913366484932 14-15 27.493858965909773 31.687735905577856 17.98274519201965 22.835659936492718 16-17 27.19429632736205 30.702174824755858 17.96477143370679 24.138757414175306 18-19 27.529806482535495 26.732969863998562 19.291833922473188 26.44538973099275 20-21 27.526810856150018 18.99227128392547 23.069318794559944 30.41159906536457 22-23 25.71445689293631 19.657300341501408 25.348990473908096 29.279252291654185 24-25 28.7520220478102 21.631418129530886 26.78689113893715 22.829668683721767 26-27 27.940207297345875 27.197291953747527 23.246060751303098 21.6164399976035 28-29 32.16404050086873 26.93966808459649 19.21694326283626 21.679348151698523 30-31 25.726613531491584 34.28417330856355 18.703182117816265 21.286031042128602 32-33 25.25185796862097 31.08505367464905 20.94467382328654 22.718414533443436 34-35 23.326502732240435 26.791135397692774 24.39663023679417 25.485731633272618 36-37 23.841624712006144 26.636231760389112 23.5770970219302 25.945046505674547 38-39 24.453770013228116 26.05938968206906 22.907448798065957 26.579391506636867 40-41 23.36358833981655 26.754070018729294 23.522066945204823 26.36027469624934 42-43 23.054519442031577 26.95825456031884 23.473506719125236 26.513719278524345 44-45 22.584899439498844 26.024837894274093 24.89834047697549 26.491922189251564 46-47 22.801186943620177 25.833827893175076 24.373887240356083 26.991097922848667 48-49 22.954371654346165 26.12796329339791 23.821055314810096 27.09660973744583 50-51 22.943221320973347 25.642423829323153 24.286006407197874 27.128348442505622 52-53 23.062825306062607 25.430686899787407 24.660948610805658 26.84553918334433 54-55 22.277738123562536 26.306606392259496 24.561820921563964 26.853834562614004 56-57 23.92241379310345 25.586206896551722 24.85344827586207 25.637931034482758 58-59 23.02428049123465 25.780444361113712 25.09609074716415 26.099184400487484 60-61 24.218106995884774 26.049382716049386 24.022633744855966 25.709876543209877 62-63 22.951197053407 25.633057090239408 25.011510128913443 26.404235727440145 64-65 23.04973821989529 26.439790575916227 23.79581151832461 26.71465968586387 66-67 22.85925925925926 27.45185185185185 24.962962962962962 24.725925925925925 68-69 24.97113640112156 25.630875804057396 23.008411677387432 26.389576117433617 70-71 23.251522421110906 27.016054622624104 24.617088023620596 25.1153349326444 72-73 24.17162276975361 25.679694137638066 23.980458793542905 26.168224299065418 74-75 24.47827154431623 24.257304198379572 25.58310827399951 25.681315983304685 76-77 24.124742571344512 24.59546925566343 25.684024713150926 25.59576345984113 78-79 23.816107846526098 25.786380919460765 24.230902177670238 26.1666090563429 80-81 26.45107794361526 24.834162520729684 24.461028192371476 24.253731343283583 82-83 24.845679012345677 24.17695473251029 26.337448559670783 24.63991769547325 84-85 24.013840830449826 25.259515570934255 26.920415224913498 23.806228373702425 86-87 25.227963525835868 22.796352583586625 25.329280648429588 26.646403242147926 88-89 23.40764331210191 20.85987261146497 25.318471337579616 30.4140127388535 90-91 18.596491228070175 26.666666666666668 27.017543859649123 27.719298245614034 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 1.5 14 2.5 15 3.0 16 4.5 17 6.5 18 11.0 19 18.0 20 18.5 21 16.0 22 18.5 23 21.5 24 30.0 25 46.0 26 58.0 27 60.0 28 67.5 29 91.0 30 121.0 31 146.5 32 173.5 33 225.0 34 258.0 35 278.5 36 331.5 37 398.5 38 460.5 39 552.0 40 685.5 41 824.5 42 1020.0 43 1255.0 44 1513.5 45 1749.0 46 1900.5 47 2073.0 48 2152.0 49 2084.0 50 1951.5 51 1772.5 52 1609.0 53 1375.5 54 1166.0 55 1059.0 56 925.5 57 825.0 58 756.0 59 676.5 60 612.5 61 551.5 62 515.5 63 487.5 64 462.5 65 436.5 66 401.5 67 364.0 68 326.0 69 285.0 70 245.0 71 214.0 72 190.5 73 169.0 74 143.5 75 120.0 76 88.0 77 67.5 78 63.5 79 53.5 80 35.5 81 22.5 82 17.5 83 13.5 84 9.5 85 7.0 86 6.0 87 4.5 88 4.5 89 3.5 90 1.5 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.023965011083817624 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.1139055950428285 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 1013.0 32-33 2075.0 34-35 1616.0 36-37 874.0 38-39 563.0 40-41 588.0 42-43 677.0 44-45 710.0 46-47 597.0 48-49 514.0 50-51 510.0 52-53 525.0 54-55 507.0 56-57 479.0 58-59 468.0 60-61 473.0 62-63 558.0 64-65 474.0 66-67 356.0 68-69 325.0 70-71 352.0 72-73 315.0 74-75 353.0 76-77 273.0 78-79 221.0 80-81 245.0 82-83 236.0 84-85 256.0 86-87 180.0 88-89 170.0 90-91 188.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.13725960098256 #Duplication Level Percentage of deduplicated Percentage of total 1 99.3896174533148 98.53214307111617 2 0.45325436635039584 0.8986879156431611 3 0.10273765637275639 0.3055538913186747 4 0.024173566205354446 0.0958600443352705 5 0.018130174654015833 0.0898687915643161 6 0.0060433915513386114 0.03594751662572644 7 0.0060433915513386114 0.04193876939668085 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.047930022167635256 0.0 0.0 0.0 0.0 2 0.05392127493858966 0.0 0.0 0.0 0.0 3 0.06590378048049847 0.0 0.0 0.0 0.0 4 0.13779881373195135 0.0 0.0 0.0 0.0 5 0.22766760529626745 0.0 0.0 0.0 0.0 6 0.5571865076987598 0.0 0.0 0.0 0.0 7 1.545743214906237 0.0 0.0 0.0 0.0 8 10.376849799293032 0.0 0.0 0.0 0.0 9 10.898088790366065 0.0 0.0 0.0 0.0 10-11 10.928045054220837 0.0 0.0 0.0 0.0 12-13 10.934036306991793 0.0 0.0 0.0 0.0 14-15 10.93703193337727 0.0 0.0 0.0 0.0 16-17 10.940027559762747 0.0 0.0 0.0 0.0 18-19 10.940027559762747 0.0 0.0 0.0 0.0 20-21 10.946018812533701 0.0 0.0 0.0 0.0 22-23 10.952010065304655 0.0 0.0 0.0 0.0 24-25 10.952010065304655 0.0 0.0 0.0 0.0 26-27 10.952010065304655 0.0 0.0 0.0 0.0 28-29 10.984961955544904 0.0 0.0 0.0 0.0 30-31 11.005931340243245 0.0 0.0 0.0 0.0 32-33 11.0119225930142 0.0 0.0 0.0 0.0 34-35 11.0119225930142 0.0 0.0 0.0 0.0 36-37 11.020909472170631 0.0 0.0 0.0 0.0 38-39 11.035887604098017 0.0 0.0 0.0 0.0 40-41 11.047870109639925 0.0 0.0 0.0 0.0 42-43 11.077826373494698 0.0 0.0 0.0 0.0 44-45 11.080821999880175 0.0 0.0 0.0 0.0 46-47 11.083817626265652 0.0 0.0 0.0 0.0 48-49 11.083817626265652 0.0 0.0 0.0 0.0 50-51 11.083817626265652 0.0 0.0 0.0 0.0 52-53 11.083817626265652 0.0 0.0 0.0 0.0 54-55 11.089808879036607 0.0 0.0 0.0 0.0 56-57 11.089808879036607 0.0 0.0 0.0 0.0 58-59 11.089808879036607 0.0 0.0 0.0 0.0 60-61 11.089808879036607 0.0 0.0 0.0 0.0 62-63 11.089808879036607 0.0 0.0 0.0 0.0 64-65 11.089808879036607 0.0 0.0 0.0 0.0 66-67 11.089808879036607 0.0 0.0 0.0 0.0 68-69 11.089808879036607 0.0 0.0 0.0 0.0 70-71 11.092804505422084 0.0 0.0 0.0 0.0 72-73 11.095800131807561 0.0 0.0 0.0 0.0 74-75 11.095800131807561 0.0 0.0 0.0 0.0 76-77 11.095800131807561 0.0 0.0 0.0 0.0 78-79 11.095800131807561 0.0 0.0 0.0 0.0 80 11.095800131807561 0.0 0.0 0.0 0.0 >>END_MODULE