##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-AB_S54_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117550
Sequences flagged as poor quality	0
Sequence length	61
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.41662271373883	40.0	40.0	40.0	40.0	40.0
2	39.4362228838792	40.0	40.0	40.0	40.0	40.0
3	39.36350489153552	40.0	40.0	40.0	40.0	40.0
4	39.31896214376861	40.0	40.0	40.0	40.0	40.0
5	37.554198213526156	40.0	40.0	40.0	24.0	40.0
6	39.31807741386644	40.0	40.0	40.0	40.0	40.0
7	37.70014461931093	40.0	40.0	40.0	24.0	40.0
8	39.24273925988941	40.0	40.0	40.0	40.0	40.0
9	38.44648234793705	40.0	40.0	40.0	40.0	40.0
10	39.2091875797533	40.0	40.0	40.0	40.0	40.0
11	39.188260314759674	40.0	40.0	40.0	40.0	40.0
12	39.2877243726074	40.0	40.0	40.0	40.0	40.0
13	39.11608677158656	40.0	40.0	40.0	40.0	40.0
14	39.23909825606125	40.0	40.0	40.0	40.0	40.0
15	39.35169715014887	40.0	40.0	40.0	40.0	40.0
16	39.22824330072309	40.0	40.0	40.0	40.0	40.0
17	39.2284134410889	40.0	40.0	40.0	40.0	40.0
18	39.075661420672056	40.0	40.0	40.0	40.0	40.0
19	39.226541897065076	40.0	40.0	40.0	40.0	40.0
20	34.31264993619736	40.0	24.0	40.0	24.0	40.0
21	34.62002552105487	40.0	24.0	40.0	24.0	40.0
22	36.381760952786045	40.0	40.0	40.0	24.0	40.0
23	38.41122926414292	40.0	40.0	40.0	40.0	40.0
24	38.79418119948958	40.0	40.0	40.0	40.0	40.0
25	35.226269672479795	40.0	40.0	40.0	24.0	40.0
26	38.6060059549128	40.0	40.0	40.0	40.0	40.0
27	38.65265844321565	40.0	40.0	40.0	40.0	40.0
28	33.541573798383666	40.0	24.0	40.0	12.0	40.0
29	37.932250106337726	40.0	40.0	40.0	24.0	40.0
30	30.3354487452148	40.0	24.0	40.0	12.0	40.0
31	32.30288387920034	40.0	24.0	40.0	12.0	40.0
32	35.18778392173543	40.0	24.0	40.0	24.0	40.0
33	35.85185878349638	40.0	40.0	40.0	24.0	40.0
34	27.203606975754997	40.0	12.0	40.0	12.0	40.0
35	26.97834113143343	40.0	12.0	40.0	12.0	40.0
36	33.34022968949383	40.0	24.0	40.0	24.0	40.0
37	26.29345810293492	24.0	12.0	40.0	12.0	40.0
38	27.88869417269247	24.0	12.0	40.0	12.0	40.0
39	26.25636750319013	24.0	12.0	40.0	12.0	40.0
40	27.7218885580604	24.0	12.0	40.0	12.0	40.0
41	27.46960442364951	24.0	12.0	40.0	12.0	40.0
42	24.949689493832413	24.0	12.0	40.0	12.0	40.0
43	27.468005104210974	24.0	12.0	40.0	12.0	40.0
44	26.641497235219056	24.0	12.0	40.0	12.0	40.0
45	28.521378136962994	24.0	24.0	40.0	12.0	40.0
46	25.172062951935345	24.0	12.0	40.0	12.0	40.0
47	26.20022118247554	24.0	12.0	40.0	12.0	40.0
48	24.46087622288388	24.0	12.0	40.0	12.0	40.0
49	27.53146746065504	24.0	24.0	40.0	12.0	40.0
50	27.717294768183752	24.0	24.0	40.0	12.0	40.0
51	26.944210974053593	24.0	24.0	40.0	12.0	40.0
52	27.40107188430455	24.0	24.0	40.0	12.0	40.0
53	25.81798383666525	24.0	12.0	40.0	12.0	40.0
54	25.925274351339855	24.0	12.0	40.0	12.0	40.0
55	23.164576775840068	12.0	12.0	40.0	12.0	40.0
56	24.845971926839642	24.0	12.0	40.0	12.0	40.0
57	22.666780093577202	12.0	12.0	40.0	12.0	40.0
58	25.805563589961718	24.0	12.0	40.0	12.0	40.0
59	22.52107188430455	12.0	12.0	40.0	12.0	40.0
60	23.410871969374735	24.0	12.0	40.0	12.0	40.0
61	24.780331773713314	24.0	12.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.014486416726938955
1101	2	-0.20154685850251752
1101	3	0.10808160680314671
1101	4	0.013927246644740876
1101	5	0.23331989747710224
1101	6	-0.05515145163230528
1101	7	0.6426238508274835
1101	8	0.2914195828745534
1101	9	0.3596363463910137
1101	10	0.0656066006383611
1101	11	0.159718055546044
1101	12	-0.2233835999195577
1101	13	0.08724442118808895
1101	14	0.029950936699094655
1101	15	0.3185310482108008
1101	16	-0.05258610563858923
1101	17	0.07560892176520184
1101	18	0.06478300105283807
1101	19	0.0034847519786467274
1101	20	0.09819102488137332
1101	21	-0.16384575447610672
1101	22	0.2967690680578201
1101	23	-0.040284973707386484
1101	24	-0.08939852569251627
1101	25	0.08181640834899895
1101	26	-0.12461819493970694
1101	27	0.33361635665822575
1101	28	-0.25885130015970503
1101	29	-0.12704969931246524
1101	30	-0.3277738382860349
1101	31	0.1798852992684914
1101	32	-0.05788428321105954
1101	33	0.06237833827714212
1101	34	0.33292353419825105
1101	35	-0.01044610574158611
1101	36	-0.6120834848883092
1101	37	-0.3025429399227164
1101	38	-0.19204405896312338
1101	39	0.2331914499784986
1101	40	-0.2918185288633879
1101	41	-0.06909281591648408
1101	42	-0.27486815816095955
1101	43	-0.1487525383418351
1101	44	0.23102303993893614
1101	45	-0.4828091067848135
1101	46	-0.017645519237568408
1101	47	0.26310683612681274
1101	48	0.4141769666969246
1101	49	-0.8029638462255626
1101	50	-0.2928745241662938
1101	51	0.31654259686272823
1101	52	0.6245700003753711
1101	53	-0.8634326274097432
1101	54	-0.15953238834999794
1101	55	0.3387189939446493
1101	56	0.46717104358816997
1101	57	0.8989134635726188
1101	58	-0.2920530909857213
1101	59	-0.024397743624074764
1101	60	-0.04578555251953986
1101	61	0.42589300637138194
1102	1	-0.07522853278606334
1102	2	0.20765100883134835
1102	3	0.2702952904179554
1102	4	-0.1279427083786402
1102	5	-0.500960625562243
1102	6	0.02098115492653818
1102	7	-0.20692787228335163
1102	8	0.11047060601815417
1102	9	0.25645819728309505
1102	10	0.05309654791742702
1102	11	0.08498538345835271
1102	12	-0.07558626277363345
1102	13	-0.23813609291550364
1102	14	-0.4700937420606266
1102	15	-0.2251201000333225
1102	16	-0.24664085201216324
1102	17	0.19606285796393053
1102	18	-0.2283394485346264
1102	19	0.19009303839927583
1102	20	0.0959868727353026
1102	21	-1.0537870763716768
1102	22	-0.21215179096579817
1102	23	0.32965515675459045
1102	24	0.24768782345709894
1102	25	0.2612760932148106
1102	26	0.1176002544094743
1102	27	-0.01949477431011104
1102	28	0.17286466207185214
1102	29	0.41389093750878914
1102	30	0.6776173987198923
1102	31	1.028603018864601
1102	32	-0.06455631132889295
1102	33	-0.024983529890732825
1102	34	1.8091693269483784
1102	35	1.361430104308159
1102	36	7.303833987108987E-4
1102	37	1.581798644088245
1102	38	0.9156615377961792
1102	39	2.0876280508184593
1102	40	1.0892317198483745
1102	41	1.146080090883622
1102	42	1.3231123618998062
1102	43	1.7709746233654862
1102	44	1.1296618270595395
1102	45	0.5405132057877893
1102	46	1.2411636428867574
1102	47	1.6951802284361115
1102	48	2.05260665722205
1102	49	-0.508232961288261
1102	50	0.8453913442398644
1102	51	1.183965823860614
1102	52	0.9270154178239132
1102	53	1.2168997825625567
1102	54	1.2238411558670812
1102	55	1.7149141507670933
1102	56	0.8565315416074135
1102	57	1.5666226346326972
1102	58	0.876207118110873
1102	59	0.8451998496800712
1102	60	0.570721759904135
1102	61	0.9429751855034247
1103	1	-0.04270625982057652
1103	2	-0.10831814591897171
1103	3	-0.0567541967423395
1103	4	-0.2706993154011741
1103	5	-0.22224534518491623
1103	6	-0.026903849191157292
1103	7	0.3199269603886563
1103	8	-0.21930404397810577
1103	9	-0.03606257834017157
1103	10	-0.34938904016372874
1103	11	-0.18965712608720509
1103	12	-0.05564166443568297
1103	13	-0.0038918763998196937
1103	14	-0.42828794734624154
1103	15	-0.32139920462267213
1103	16	-0.2629319353392603
1103	17	-0.07992725957743119
1103	18	-0.1995006374243502
1103	19	-0.18454198443519232
1103	20	-0.6560083357696058
1103	21	0.2060467186421704
1103	22	0.17110792304183775
1103	23	0.38308032707727335
1103	24	-0.07480974336179713
1103	25	0.1539466030598362
1103	26	0.07265004103820871
1103	27	-0.24499451227521973
1103	28	-1.3753871908891426
1103	29	0.012154020536421228
1103	30	-0.3273088571758933
1103	31	-0.4115706728326387
1103	32	0.174838762415213
1103	33	0.08324436559477988
1103	34	0.16925018342812592
1103	35	0.4933609270977044
1103	36	-0.5210634325779324
1103	37	0.08775057940306041
1103	38	-0.1056156951154037
1103	39	-0.1631468237791296
1103	40	0.12712945137491616
1103	41	-0.687924550877252
1103	42	-0.378617068361482
1103	43	-0.23866826051562384
1103	44	0.3982999943126657
1103	45	-0.828057581065547
1103	46	-0.23758158433492582
1103	47	-0.5118423065642652
1103	48	-0.13153648869394985
1103	49	-0.9393776794135889
1103	50	-1.030508932768445
1103	51	-0.681423110780397
1103	52	-0.48836228675046556
1103	53	-0.7499191138962296
1103	54	-0.4635137891055905
1103	55	-0.7114989659507316
1103	56	0.33476767247512385
1103	57	-0.8647017645587383
1103	58	-0.14279415463001044
1103	59	-0.334017040340143
1103	60	-0.6862970317406969
1103	61	-0.17230519182681547
1104	1	-0.1240285269696173
1104	2	-0.036785195645862245
1104	3	0.06727219185098221
1104	4	0.06091734062493259
1104	5	-1.224643018448731
1104	6	-0.005367352064105546
1104	7	-1.658451417989724
1104	8	0.1873640414301363
1104	9	-0.8927554917259997
1104	10	-0.06308699008215513
1104	11	-0.22424604571549622
1104	12	0.17742920170604748
1104	13	0.023574960515830412
1104	14	-0.15123609234163382
1104	15	-0.13426719071900806
1104	16	-0.062492125450631875
1104	17	-0.23156235924403035
1104	18	0.16985454452925808
1104	19	0.13751235336717826
1104	20	-0.0652159685992828
1104	21	0.030718634652671994
1104	22	0.11101002987307851
1104	23	-0.22113333540410451
1104	24	-0.3526277188175655
1104	25	-0.13366068927186348
1104	26	0.07892087880510701
1104	27	-0.33465897989244553
1104	28	-0.05141755195887043
1104	29	-0.010971467155997061
1104	30	0.7463096432142606
1104	31	-0.190786108740987
1104	32	0.552394270679379
1104	33	-0.30177372336031283
1104	34	0.4970078623669103
1104	35	0.5332843150525903
1104	36	-0.2959375192630418
1104	37	1.125701916566996
1104	38	1.0155590229097093
1104	39	1.1358245885781102
1104	40	1.273671268782877
1104	41	0.7612348447388371
1104	42	1.3228797040014335
1104	43	0.5066534391367838
1104	44	0.9068337248936409
1104	45	1.3829092913186507
1104	46	1.5681643190487726
1104	47	0.7573071578607902
1104	48	1.155943116895891
1104	49	1.525458810619753
1104	50	1.313848022612131
1104	51	1.8010908992926673
1104	52	0.4120562420145184
1104	53	1.464651362091569
1104	54	0.5337196648207758
1104	55	1.32195496041658
1104	56	1.5444718648564795
1104	57	1.5981768620994181
1104	58	1.6436001136540206
1104	59	1.18168133520075
1104	60	2.29660713226583
1104	61	1.4127696467913289
1201	1	0.3016533171476965
1201	2	0.17070653093179544
1201	3	0.14618352358668574
1201	4	0.2024499207915369
1201	5	-0.12841455787586398
1201	6	0.08860731414609546
1201	7	0.4502442341841899
1201	8	0.23181228974692658
1201	9	-0.12084518703581892
1201	10	0.05719145337328513
1201	11	0.17456144030527554
1201	12	-0.09259818617481841
1201	13	-0.009062264178908208
1201	14	-0.18498594969641857
1201	15	0.042584344143485
1201	16	-0.05165108927580064
1201	17	0.11067203536968861
1201	18	-0.02100475023281234
1201	19	-0.2659168375491703
1201	20	-1.603188124253741
1201	21	-0.5178663248360849
1201	22	0.0440808960148118
1201	23	-0.26503953191215146
1201	24	0.2986332648638239
1201	25	-0.39270439576507243
1201	26	-0.010546198679769248
1201	27	-0.07989462978403594
1201	28	-0.7975376021699887
1201	29	-0.4993030887467782
1201	30	-1.3920562132275975
1201	31	-0.6942381228913952
1201	32	-0.5029520718973615
1201	33	0.0065111106006554564
1201	34	-0.9309848341981954
1201	35	-0.7690950070973592
1201	36	-1.1899235970902708
1201	37	-0.9090179282350093
1201	38	-0.8660739794867318
1201	39	-0.4333583396428189
1201	40	-0.5798035686015837
1201	41	-0.33522184817454814
1201	42	-0.679145858020707
1201	43	-1.1447787187999943
1201	44	-1.019561345287805
1201	45	-0.27488484311020045
1201	46	-0.3838213023137733
1201	47	-2.099562635588942
1201	48	-0.10709465555646602
1201	49	-1.0337960107884392
1201	50	-0.15659588929018398
1201	51	-0.10327975002834222
1201	52	-0.504696011779842
1201	53	-0.76560654045322
1201	54	-0.19471237899983151
1201	55	-0.034001903671061484
1201	56	0.33135991689345445
1201	57	-0.6474867234306529
1201	58	-0.5027824037486965
1201	59	-0.24482785115095496
1201	60	0.024925059916178327
1201	61	-0.4183098921793409
1202	1	-0.15081436792868885
1202	2	0.09708725948643604
1202	3	0.14865120866306825
1202	4	0.042814198112338886
1202	5	-0.5681912911308586
1202	6	0.027150204862898875
1202	7	-0.004397363935666476
1202	8	0.007722983048921606
1202	9	0.2882617459841512
1202	10	0.4289893382146488
1202	11	0.17791044148036406
1202	12	0.36597995718593523
1202	13	-0.14443241694036146
1202	14	0.40414448508619216
1202	15	0.3704926872692198
1202	16	-0.025094097501423107
1202	17	0.04980247015230077
1202	18	0.36266152473780977
1202	19	0.5830255831323754
1202	20	-0.25600833576960014
1202	21	0.42226293485838795
1202	22	-0.1964596445257314
1202	23	-0.11421697022002775
1202	24	0.02248755393550539
1202	25	-0.5703777212644852
1202	26	0.20237977076794067
1202	27	0.12257305529234941
1202	28	-0.7807925962945461
1202	29	0.585126993509391
1202	30	-0.6516331815002161
1202	31	0.3451860839241192
1202	32	-0.05218826461181436
1202	33	-0.02486374251332535
1202	34	-0.27399305981511546
1202	35	0.12579335953013882
1202	36	-0.36971208122658084
1202	37	-0.06360077194828762
1202	38	1.688978899479192
1202	39	-0.5307143913466952
1202	40	0.2676699919154579
1202	41	-0.49332995628265763
1202	42	-0.05429274403715567
1202	43	-0.41164123348859505
1202	44	0.19289458890726152
1202	45	-0.09292244593041232
1202	46	-0.5619059086592486
1202	47	-0.836166630888588
1202	48	-0.14234729950475966
1202	49	-1.0042425442784513
1202	50	-0.7061846084441221
1202	51	-0.3246663540236412
1202	52	-0.07755147593965361
1202	53	-0.7391083030854197
1202	54	-1.4689191945109954
1202	55	-0.12771518216694844
1202	56	0.5834163211237708
1202	57	-0.38902608888306034
1202	58	0.28963827780242113
1202	59	-0.13942244574554863
1202	60	0.3407299952863312
1202	61	-0.7128457323673558
1203	1	0.014125991425082418
1203	2	-0.04103745740923159
1203	3	0.010526491767400614
1203	4	0.05531608547455846
1203	5	-0.045769427926870776
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2403	27	0.061119087956029716
2403	28	0.38584998211273813
2403	29	0.13095731581029924
2403	30	0.8375415547333418
2403	31	0.10191393476961252
2403	32	0.2466410459822015
2403	33	0.4481525893265328
2403	34	0.05634369405105133
2403	35	-0.00619103127778331
2403	36	0.49573090186346036
2403	37	0.04930571742878698
2403	38	0.2037353671768365
2403	39	-0.5190942150206368
2403	40	0.16002437838749017
2403	41	0.6714626438040625
2403	42	0.4053112449785985
2403	43	-0.017017732982743894
2403	44	0.03344958240343132
2403	45	0.5753244105517403
2403	46	-0.16369807515729917
2403	47	0.5057035815698612
2403	48	-0.3400637325153113
2403	49	0.6474990368748941
2403	50	0.7470311741834266
2403	51	0.10597391191074124
2403	52	0.3566153111683086
2403	53	-0.22706899104610656
2403	54	-0.3079999865329661
2403	55	0.9392020023972947
2403	56	0.22382678506364684
2403	57	1.413719972686529
2403	58	-0.2792878675943129
2403	59	0.228434180934606
2403	60	-0.791803607835508
2403	61	0.5364099404177303
2404	1	0.0516533171476965
2404	2	-0.03581520819864181
2404	3	0.1896617844562556
2404	4	0.039406442530669494
2404	5	0.3172376160371755
2404	6	0.2299116619721815
2404	7	0.5045920602711433
2404	8	0.11224707235562192
2404	9	0.020459160790267106
2404	10	0.035452322938503755
2404	11	0.03325709247918951
2404	12	-0.0056416644356858114
2404	13	0.12137251842978714
2404	14	0.10849231117314417
2404	15	0.053453909360875684
2404	16	-0.1712163066671053
2404	17	0.14328073102186067
2404	18	0.3811691628106644
2404	19	-0.015916837549170282
2404	20	0.2337683974853917
2404	21	0.3625684577726105
2404	22	-0.1189625822460556
2404	23	0.6697430767834973
2404	24	0.3203723952986053
2404	25	-0.023139178373767777
2404	26	0.47858423610284007
2404	27	0.34401841369422925
2404	28	0.8002884847865346
2404	29	0.5985229982097451
2404	30	0.6840307432941444
2404	31	0.5883705727607804
2404	32	0.7796566237548106
2404	33	1.4956415453832648
2404	34	-0.9418543994155861
2404	35	-0.3125732679669255
2404	36	0.2557285768227757
2404	37	-0.03945271084370816
2404	38	0.0360999335567449
2404	39	-0.19422790486020958
2404	40	-0.9493687859928848
2404	41	-0.06348271773976677
2404	42	-0.4617545536728791
2404	43	0.1269604116347871
2404	44	0.31739517645132764
2404	45	-0.003145712675415524
2404	46	0.23574391507753134
2404	47	0.8352199731067103
2404	48	-0.6505729164260288
2404	49	0.9879431196463422
2404	50	0.10427367592720671
2404	51	0.005415902145571749
2404	52	0.495303988220158
2404	53	-0.11343262740974325
2404	54	0.7835484905653836
2404	55	0.42251983545937577
2404	56	-0.17950964832393623
2404	57	-0.5822693321263053
2404	58	0.2146089005991314
2404	59	0.40734606189252176
2404	60	0.090142451220526
2404	61	0.15777706434239747
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	5.0
15	27.0
16	99.0
17	181.0
18	231.0
19	285.0
20	385.0
21	452.0
22	634.0
23	998.0
24	1497.0
25	2414.0
26	4743.0
27	12527.0
28	21032.0
29	10709.0
30	4883.0
31	3847.0
32	4201.0
33	3258.0
34	2229.0
35	2673.0
36	3005.0
37	3757.0
38	7437.0
39	19225.0
40	6815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.302672996103652	69.26905211576745	13.145491977608767	10.282782910520137
2	49.363675031901316	7.643555933645257	28.52743513398554	14.465333900467886
3	8.11229264142918	49.924287537218206	12.092726499361973	29.870693321990643
4	8.313908974904297	7.167162909400256	70.99191833262441	13.527009783071033
5	6.586984262016163	8.55720969800085	12.06295193534666	72.79285410463633
6	51.17907273500638	8.531688643130583	27.470012760527435	12.819225861335601
7	50.40238196512122	8.515525308379413	11.228413441088898	29.85367928541046
8	8.659293917481921	50.67035304125904	28.23649510846448	12.433857932794554
9	7.636750319013186	52.90769885155253	10.170991067630796	29.284559761803486
10	51.56444066354743	27.327094853253936	8.99617184176946	12.112292641429178
11	9.777966822628668	67.6376010208422	9.008081667375585	13.576350489153551
12	52.010208421948114	10.991918332624415	8.578477243726073	28.4193960017014
13	9.527860484900042	27.598468736707783	9.846873670778391	53.026797107613774
14	52.31986388770736	26.546150574223738	9.020842194810719	12.113143343258189
15	9.703955763504892	11.29051467460655	51.6290940025521	27.376435559336453
16	10.272224585282858	27.314334325818802	9.910676307954063	52.502764780944275
17	52.5903870693322	26.569970225435984	9.087196937473415	11.7524457677584
18	11.118672905146747	11.239472564866015	51.4759676733305	26.16588685665674
19	53.32199064227988	27.16546150574224	9.931093151850275	9.581454700127605
20	12.2951935346661	27.68694172692471	49.3908974904296	10.626967247979584
21	12.954487452148022	54.49680986814121	7.906422798809018	24.642279880901743
22	74.16758826031476	10.07145895363675	7.424925563589961	8.336027222458528
23	69.75074436410038	15.192683964270524	7.467460655040409	7.589111016588686
24	55.45299872394725	26.853253934495957	10.529136537643556	7.164610803913228
25	30.30965546575925	51.50914504466184	7.373883453849425	10.807316035729476
26	31.75074436410038	53.798383666524884	7.234368353891961	7.2165036154827735
27	16.976605699702255	67.69034453424075	7.726924712888132	7.6061250531688644
28	31.002126754572522	54.406635474266274	6.712037430880477	7.879200340280732
29	30.053594215227562	55.714164185452994	7.348362398979158	6.88387920034028
30	19.99149298170991	65.15525308379412	7.542322415993194	7.310931518502765
31	31.28030625265844	13.224159931943854	48.21437686091025	7.281156954487453
32	74.5138239047214	11.328796256911952	7.578902594640578	6.578477243726074
33	13.796799632493684	27.58934571965733	49.005095747305376	9.608758900543602
34	17.793279455550827	42.69247128881327	7.601020842194811	31.913228413441093
35	13.430880476393025	27.487026797107617	31.35516801361123	27.726924712888135
36	16.1999149298171	26.783496384517225	6.643981284559762	50.37260740110592
37	57.079540621012335	12.729051467460655	12.058698426201616	18.132709485325393
38	16.913653764355594	26.974053594215224	46.17694598043386	9.935346660995322
39	54.05444491705658	12.63037005529562	27.934495959166313	5.380689068481497
40	58.30710336027223	27.50829434283284	8.322415993194385	5.862186303700553
41	32.01616333475117	16.177796682262866	45.97703105061676	5.829008932369205
42	35.118672905146745	28.263717566992767	31.79242875372182	4.825180774138665
43	34.06380263717567	13.484474691620587	46.92556358996172	5.5261590812420245
44	18.75457252233092	28.727350063802636	46.9902169289664	5.527860484900042
45	15.742237345810295	28.90854955338154	48.508719693747345	6.840493407060825
46	30.302849851127178	13.239472564866015	14.352190557209699	42.10548702679711
47	19.373883453849423	27.13143343258188	9.597618034878774	43.89706507868992
48	14.68396427052318	41.03700552956189	24.809868141216505	19.469162058698426
49	14.752871118672905	12.081667375584857	7.325393449595917	65.84006805614632
50	31.897065078689916	10.417694598043385	10.641429179072736	47.04381114419396
51	29.690344534240747	11.150999574649086	12.256061250531689	46.90259464057848
52	16.373458102934922	25.883453849425774	10.797958315610378	46.945129732028924
53	13.783921735431731	23.10591237771161	11.27520204168439	51.83496384517227
54	27.948957890259464	8.568268821777966	9.031901318587835	54.45087196937474
55	14.32752020416844	23.053168864313058	42.313058273075285	20.306252658443217
56	26.1616333475117	7.998298596341982	51.956614206720545	13.883453849425775
57	26.39897915780519	9.158655891110167	40.94938324117397	23.492981709910676
58	25.638451722671203	9.134836239897917	57.207996597192675	8.018715440238196
59	11.105061675882604	9.049766056997024	39.27350063802637	40.571671629094006
60	12.78179498085921	7.897065078689919	44.49340706082518	34.82773287962569
61	24.440663547426627	8.348787749893662	17.320289238621864	49.89025946405785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	3.5
13	5.0
14	3.0
15	1.0
16	2.0
17	7.5
18	12.0
19	15.5
20	20.0
21	21.0
22	27.0
23	33.0
24	33.0
25	41.0
26	49.0
27	69.5
28	90.0
29	111.5
30	136.5
31	140.0
32	143.5
33	147.0
34	175.5
35	235.0
36	266.0
37	305.0
38	344.0
39	407.5
40	605.5
41	740.0
42	1060.5
43	1978.0
44	2575.0
45	3918.0
46	5261.0
47	8659.5
48	22448.5
49	32839.0
50	25321.0
51	17803.0
52	16061.0
53	12318.5
54	10318.0
55	8458.5
56	6599.0
57	5357.0
58	3319.5
59	2524.0
60	2047.5
61	1314.0
62	1057.0
63	878.5
64	700.0
65	598.0
66	438.0
67	380.0
68	303.0
69	226.0
70	167.0
71	106.5
72	105.0
73	93.0
74	81.0
75	65.5
76	40.0
77	30.0
78	25.5
79	21.0
80	17.0
81	13.5
82	14.0
83	11.0
84	7.0
85	6.0
86	6.5
87	7.0
88	4.0
89	3.0
90	5.0
91	6.5
92	8.0
93	6.5
94	4.0
95	3.0
96	7.0
97	11.0
98	10.0
99	69.5
100	130.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0034028073160357296
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	8.507018290089324E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	117550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.618885580604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.88273545805345	42.32496809868142
2	5.073602972702586	4.831986388770735
3	1.6078319279691295	2.2968949383241175
4	0.9093182792625412	1.7320289238621864
5	0.5216521366299843	1.2420246703530413
6	0.380520222952694	1.0871969374734156
7	0.28940974703444333	0.9646958740961293
8	0.23581534943547233	0.8983411314334326
9	0.17686151207660428	0.7579753296469588
>10	1.6507074460483064	15.628243300723096
>50	0.17864799199656997	5.746490854955338
>100	0.09111047591825068	8.50957039557635
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0017864799199656994	13.979583156103786
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	16433	13.979583156103786	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTTGTTTTTCCACCCCCCTTTTCTC	473	0.40238196512122504	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTTGTTTTTCCACCCCCCTTTTCTC	450	0.38281582305401957	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTGGTGTTTCCACCCCCCTTTTCTC	405	0.3445342407486176	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTTGTTTTTCCACCCCCCTTTTCTC	400	0.340280731603573	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTATCGTGGTGTTTCCACCCCCCTTTTCTC	381	0.3241173968524032	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	349	0.2968949383241174	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTTGTTTTTCCCCCCCCCTTTTCTC	307	0.2611654615057422	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCCTTGTTTTTCCACCCCCCTTTTCTC	290	0.24670353041259038	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTTGTGTTTCCACCCCCCTTTTCTC	271	0.23054019566142064	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGTGTA	269	0.2288387920034028	Illumina Single End PCR Primer 1 (100% over 21bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTGGTGTTTCCACCCCCCTTTTCTC	260	0.22118247554232243	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTTGTTTTTCCCCCCCCCTTTTCCC	249	0.21182475542322415	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTGGTTTTTCCACCCCCCTTTTCTC	243	0.20672054444917054	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTGGTTTTTCCACCCCCCTTTTCTC	236	0.20076563164610806	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTACCGTGGTGTTTCCACCCCCCTTTTCTC	222	0.188855806039983	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	217	0.18460229689493832	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTTGTGTTTCCACCCCCCTTTTCTC	213	0.1811994895789026	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTACCGTTGTTTTTCCACCCCCCTTTTCTC	194	0.16503615482773287	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTATCGTGGTTTTTCCACCCCCCTTTTCTC	191	0.1624840493407061	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCCTTGTTTTTCCCCCCCCCTTTTCTC	188	0.1599319438536793	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTCAACAACAACAAAGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	186	0.15823054019566143	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTTGTTTTTCCACCCCCCTTTTCCC	181	0.15397703105061677	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTATCGTTGTGTTTCCACCCCCCTTTTCTC	174	0.14802211824755424	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTTGTTTTTCCCCCCCCCTTTTCTC	171	0.14547001276052743	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCTTTGTTTTTCCACCCCCCTTTTCTC	167	0.14206720544449172	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTATCGTTGTTTTTCCACCCCCCTTTTCTC	155	0.13185878349638452	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTTGTTTTTCCCCCCCCCTTTTCCC	153	0.13015737983836664	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTGGTTTTTCCACCCCCCTTTTCTC	151	0.1284559761803488	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAGATCGGAAGAGCGTCGT	150	0.12760527435133986	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTTGTTTTTCCCCCCCCCTTTTCCC	145	0.12335176520629519	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTACCGTGGTTTTTCCACCCCCCTTTTCTC	138	0.11739685240323267	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGATCGGAAGAGCGTCGTGT	136	0.11569544874521481	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTTGTTTTTCCACCCCCCCTTTCTC	134	0.11399404508719695	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTACCCTTGTTTTTCCACCCCCCTTTTCTC	133	0.113143343258188	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTATCGTGGTGTTTCCACCCCCCTTGTCTC	133	0.113143343258188	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTGGTGTTTCCACCCCCCTTTTTTC	130	0.11059123777116121	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTTGTTTTTCCACCCCCCTTTTCCC	129	0.10974053594215227	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTGGTGTTTCCACCCCCCTTGTCTC	128	0.10888983411314335	Illumina Single End PCR Primer 1 (96% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	128	0.10888983411314335	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCTTTGTTTTTCCACCCCCCTTTTCTC	127	0.10803913228413442	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCCTGGTTTTTCCACCCCCCTTTTCTC	125	0.10633772862611655	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTACCGTTGTGTTTCCACCCCCCTTTTCTC	123	0.10463632496809869	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCCTTGTTTTTCCCCCCCCCTTTTCCC	123	0.10463632496809869	Illumina Single End PCR Primer 1 (96% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	41.79327945555083	0.0	0.0	0.0	0.0
2	41.93619736282433	0.0	0.0	0.0	0.0
3	41.99744789451297	0.0	0.0	0.0	0.0
4	42.05444491705657	0.0	0.0	0.0	0.0
5	42.123351765206294	0.0	0.0	0.0	0.0
6	42.30114844746916	0.0	0.0	0.0	0.0
7	42.501063377286265	0.0	0.0	0.0	0.0
8	42.92471288813271	0.0	0.0	0.0	0.0
9	43.25053168864313	0.0	0.0	0.0	0.0
10	43.702254359846876	0.0	0.0	0.0	0.0
11	44.16418545299872	0.0	0.0	0.0	0.0
12	44.78009357720119	0.0	0.0	0.0	0.0
13	45.54572522330923	0.0	0.0	0.0	0.0
14	46.3623989791578	0.0	0.0	0.0	0.0
15	47.43343258188005	0.0	0.0	0.0	0.0
16	48.699276903445345	0.0	0.0	0.0	0.0
17	49.709910676307956	0.0	0.0	0.0	0.0
18	50.85580603998299	0.0	0.0	0.0	0.0
19	51.6376010208422	0.0	0.0	0.0	0.0
20	52.571671629094006	0.0	0.0	0.0	0.0
21	56.53083794130158	0.0	0.0	0.0	0.0
22	56.94938324117397	0.0	0.0	0.0	0.0
23	57.62994470438112	0.0	0.0	0.0	0.0
24	58.36324968098681	0.0	0.0	0.0	0.0
25	58.75882603147597	0.0	0.0	0.0	0.0
26	59.32199064227988	0.0	0.0	0.0	0.0
27	59.76945980433858	0.0	0.0	0.0	0.0
28	60.28668651637601	0.0	0.0	0.0	0.0
29	60.93917481922586	0.0	0.0	0.0	0.0
30	61.60187154402382	0.0	0.0	0.0	0.0
31	62.30370055295619	0.0	0.0	0.0	0.0
32	63.09910676307954	0.0	0.0	0.0	0.0
33	63.90642279880902	0.0	0.0	0.0	0.0
34	64.61420672054444	0.0	0.0	0.0	0.0
35	65.19608677158656	0.0	0.0	0.0	0.0
36	65.68353891960868	0.0	0.0	0.0	0.0
37	66.01786473840919	0.0	0.0	0.0	0.0
38	66.25435984687367	0.0	0.0	0.0	0.0
39	66.52828583581454	0.0	0.0	0.0	0.0
40	66.74521480221182	0.0	0.0	0.0	0.0
41	67.4955338153977	0.0	0.0	0.0	0.0
42	67.95746490854955	0.0	0.0	0.0	0.0
43	68.3139089749043	0.0	0.0	0.0	0.0
44	69.83666524883029	0.0	0.0	0.0	0.0
45	85.49128030625266	0.0	0.0	0.0	0.0
46	85.95831561037856	0.0	0.0	0.0	0.0
47	86.17439387494683	0.0	0.0	0.0	0.0
48	86.4006805614632	0.0	0.0	0.0	0.0
49	86.59549128030625	0.0	0.0	0.0	0.0
50	86.75627392598894	0.0	0.0	0.0	0.0
>>END_MODULE