AM-AL-AB_S54_R2_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	AM-AL-AB_S54_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117550
Sequences flagged as poor quality	0
Sequence length	61
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	16433	13.979583156103786	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTTGTTTTTCCACCCCCCTTTTCTC	473	0.40238196512122504	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTTGTTTTTCCACCCCCCTTTTCTC	450	0.38281582305401957	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTGGTGTTTCCACCCCCCTTTTCTC	405	0.3445342407486176	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTTGTTTTTCCACCCCCCTTTTCTC	400	0.340280731603573	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTATCGTGGTGTTTCCACCCCCCTTTTCTC	381	0.3241173968524032	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	349	0.2968949383241174	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTTGTTTTTCCCCCCCCCTTTTCTC	307	0.2611654615057422	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCCTTGTTTTTCCACCCCCCTTTTCTC	290	0.24670353041259038	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTTGTGTTTCCACCCCCCTTTTCTC	271	0.23054019566142064	Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGTGTA	269	0.2288387920034028	Illumina Single End PCR Primer 1 (100% over 21bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTGGTGTTTCCACCCCCCTTTTCTC	260	0.22118247554232243	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTTGTTTTTCCCCCCCCCTTTTCCC	249	0.21182475542322415	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTGGTTTTTCCACCCCCCTTTTCTC	243	0.20672054444917054	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTGGTTTTTCCACCCCCCTTTTCTC	236	0.20076563164610806	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTACCGTGGTGTTTCCACCCCCCTTTTCTC	222	0.188855806039983	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCGT	217	0.18460229689493832	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTTGTGTTTCCACCCCCCTTTTCTC	213	0.1811994895789026	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTACCGTTGTTTTTCCACCCCCCTTTTCTC	194	0.16503615482773287	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTATCGTGGTTTTTCCACCCCCCTTTTCTC	191	0.1624840493407061	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCCTTGTTTTTCCCCCCCCCTTTTCTC	188	0.1599319438536793	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTCAACAACAACAAAGGAGGAGGAGGAAAAGAGAGAGAAGATCGGAAGAGCGTCG	186	0.15823054019566143	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTTGTTTTTCCACCCCCCTTTTCCC	181	0.15397703105061677	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTATCGTTGTGTTTCCACCCCCCTTTTCTC	174	0.14802211824755424	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTTGTTTTTCCCCCCCCCTTTTCTC	171	0.14547001276052743	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCTTTGTTTTTCCACCCCCCTTTTCTC	167	0.14206720544449172	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTATCGTTGTTTTTCCACCCCCCTTTTCTC	155	0.13185878349638452	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTTGTTTTTCCCCCCCCCTTTTCCC	153	0.13015737983836664	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTGGTTTTTCCACCCCCCTTTTCTC	151	0.1284559761803488	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGAGATCGGAAGAGCGTCGT	150	0.12760527435133986	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTTGTTTTTCCCCCCCCCTTTTCCC	145	0.12335176520629519	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTACCGTGGTTTTTCCACCCCCCTTTTCTC	138	0.11739685240323267	Illumina Single End PCR Primer 1 (96% over 29bp)
ATCTCTCTCAACAACAACAACGGAGGAGGAGGAAAAGAGAGAGATCGGAAGAGCGTCGTGT	136	0.11569544874521481	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCTTGTTTTTCCACCCCCCCTTTCTC	134	0.11399404508719695	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTACCCTTGTTTTTCCACCCCCCTTTTCTC	133	0.113143343258188	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTATCGTGGTGTTTCCACCCCCCTTGTCTC	133	0.113143343258188	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTGGTGTTTCCACCCCCCTTTTTTC	130	0.11059123777116121	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCGTTGTTTTTCCACCCCCCTTTTCCC	129	0.10974053594215227	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCGTGGTGTTTCCACCCCCCTTGTCTC	128	0.10888983411314335	Illumina Single End PCR Primer 1 (96% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	128	0.10888983411314335	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCTTTGTTTTTCCACCCCCCTTTTCTC	127	0.10803913228413442	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCCTGGTTTTTCCACCCCCCTTTTCTC	125	0.10633772862611655	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTACCGTTGTGTTTCCACCCCCCTTTTCTC	123	0.10463632496809869	Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCTCCTTGTTTTTCCCCCCCCCTTTTCCC	123	0.10463632496809869	Illumina Single End PCR Primer 1 (96% over 29bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content