##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-AB_S54_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117550
Sequences flagged as poor quality	0
Sequence length	61
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.34050191407911	40.0	40.0	40.0	40.0	40.0
2	39.12835389196087	40.0	40.0	40.0	40.0	40.0
3	38.99014887282008	40.0	40.0	40.0	40.0	40.0
4	38.51627392598894	40.0	40.0	40.0	40.0	40.0
5	39.32801361122927	40.0	40.0	40.0	40.0	40.0
6	39.0914164185453	40.0	40.0	40.0	40.0	40.0
7	39.18193109315185	40.0	40.0	40.0	40.0	40.0
8	39.066950233943004	40.0	40.0	40.0	40.0	40.0
9	39.22582730752871	40.0	40.0	40.0	40.0	40.0
10	39.19812845597618	40.0	40.0	40.0	40.0	40.0
11	39.32845597618035	40.0	40.0	40.0	40.0	40.0
12	39.349145044661846	40.0	40.0	40.0	40.0	40.0
13	39.134529987239475	40.0	40.0	40.0	40.0	40.0
14	39.281837515950656	40.0	40.0	40.0	40.0	40.0
15	39.484678860059546	40.0	40.0	40.0	40.0	40.0
16	39.00821777966823	40.0	40.0	40.0	40.0	40.0
17	39.4743343258188	40.0	40.0	40.0	40.0	40.0
18	39.382220331773716	40.0	40.0	40.0	40.0	40.0
19	38.52770735857082	40.0	40.0	40.0	40.0	40.0
20	38.271918332624416	40.0	40.0	40.0	24.0	40.0
21	39.40192258613356	40.0	40.0	40.0	40.0	40.0
22	38.69260740110591	40.0	40.0	40.0	40.0	40.0
23	38.49048064653339	40.0	40.0	40.0	40.0	40.0
24	39.28694172692471	40.0	40.0	40.0	40.0	40.0
25	39.36166737558486	40.0	40.0	40.0	40.0	40.0
26	39.309740535942154	40.0	40.0	40.0	40.0	40.0
27	39.24529136537644	40.0	40.0	40.0	40.0	40.0
28	39.17383241173969	40.0	40.0	40.0	40.0	40.0
29	39.235491280306256	40.0	40.0	40.0	40.0	40.0
30	39.14106337728626	40.0	40.0	40.0	40.0	40.0
31	39.168285835814544	40.0	40.0	40.0	40.0	40.0
32	39.31504891535517	40.0	40.0	40.0	40.0	40.0
33	39.10404083368779	40.0	40.0	40.0	40.0	40.0
34	39.19537218205019	40.0	40.0	40.0	40.0	40.0
35	39.12486601446193	40.0	40.0	40.0	40.0	40.0
36	39.11118672905147	40.0	40.0	40.0	40.0	40.0
37	39.07535516801361	40.0	40.0	40.0	40.0	40.0
38	39.09226712037431	40.0	40.0	40.0	40.0	40.0
39	37.67242875372182	40.0	40.0	40.0	40.0	40.0
40	38.859481071884304	40.0	40.0	40.0	40.0	40.0
41	38.823615482773285	40.0	40.0	40.0	40.0	40.0
42	38.93999149298171	40.0	40.0	40.0	40.0	40.0
43	38.90882177796682	40.0	40.0	40.0	40.0	40.0
44	39.06946831135687	40.0	40.0	40.0	40.0	40.0
45	37.9771671629094	40.0	40.0	40.0	24.0	40.0
46	38.16735006380264	40.0	40.0	40.0	40.0	40.0
47	38.663955763504894	40.0	40.0	40.0	40.0	40.0
48	38.91831561037856	40.0	40.0	40.0	40.0	40.0
49	38.79986388770736	40.0	40.0	40.0	40.0	40.0
50	38.7331348362399	40.0	40.0	40.0	40.0	40.0
51	38.59896214376861	40.0	40.0	40.0	40.0	40.0
52	38.5357039557635	40.0	40.0	40.0	40.0	40.0
53	38.34579327945555	40.0	40.0	40.0	40.0	40.0
54	38.0742492556359	40.0	40.0	40.0	40.0	40.0
55	37.96753721820502	40.0	40.0	40.0	24.0	40.0
56	37.79361973628243	40.0	40.0	40.0	24.0	40.0
57	37.31569544874522	40.0	40.0	40.0	24.0	40.0
58	37.09737133134836	40.0	40.0	40.0	24.0	40.0
59	36.31282007656316	40.0	40.0	40.0	24.0	40.0
60	35.94273075287112	40.0	40.0	40.0	24.0	40.0
61	34.929749042960445	40.0	24.0	40.0	24.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.23850111523326234
1101	2	-0.27885046190087337
1101	3	0.004202340597203147
1101	4	-0.44307937580550316
1101	5	0.36773290303145245
1101	6	0.1862357679527591
1101	7	0.014761178551317755
1101	8	-0.10795045082934251
1101	9	-0.11299542493554071
1101	10	0.10501584262707553
1101	11	0.16621487021054548
1101	12	-0.22232079179074304
1101	13	0.06449999352835079
1101	14	-0.190248211845514
1101	15	-0.2057355096530955
1101	16	-0.13691483257358072
1101	17	-0.010118122190910128
1101	18	0.05966482841139964
1101	19	0.18052179751904873
1101	20	0.26245208965604405
1101	21	-0.24064777175208008
1101	22	-1.567556319059804E-4
1101	23	0.2941511635298468
1101	24	-0.30438399739965405
1101	25	-0.3357359534939093
1101	26	-0.29396504379415234
1101	27	-0.12496770974509985
1101	28	-0.09187827510334046
1101	29	0.1829212782512002
1101	30	-2.3478110456665036E-4
1101	31	-0.32065038012825653
1101	32	-0.23401177374312
1101	33	-0.15667038240918174
1101	34	-0.0850904652839759
1101	35	-0.2726697288211355
1101	36	0.04695603897774703
1101	37	0.03576296799774781
1101	38	-0.13803167603342814
1101	39	1.203015411887641
1101	40	0.027631654502606295
1101	41	-0.11657358000551454
1101	42	-0.16818074727033405
1101	43	-0.3869013109204431
1101	44	-0.1306292115976433
1101	45	-0.5696364808652845
1101	46	-0.3055311593950165
1101	47	-0.4694307536936648
1101	48	-4.246781058938609E-4
1101	49	-0.13227562438626705
1101	50	-0.20299306449125964
1101	51	-0.24134456939373194
1101	52	0.02899493470950887
1101	53	-0.2524712817887149
1101	54	-0.9709305288227483
1101	55	0.10525355646600332
1101	56	-0.5614076429011519
1101	57	-0.7261251010596865
1101	58	-0.4631246061069305
1101	59	-0.43568474584438377
1101	60	0.11853754334745048
1101	61	-0.38541340936131974
1102	1	0.1823845930279191
1102	2	0.056627451898904724
1102	3	0.20641840707919812
1102	4	0.1373274987663322
1102	5	0.05840695658639561
1102	6	-0.31348126656902764
1102	7	0.13357689356040225
1102	8	-0.051744571104563875
1102	9	-0.04210512618357143
1102	10	-0.039475266299739076
1102	11	0.09443099626422224
1102	12	-0.05039692439729748
1102	13	-0.050026560547841825
1102	14	-0.018651988690002952
1102	15	0.23703099914862236
1102	16	-0.03966406558820523
1102	17	-0.06006897555522528
1102	18	0.16385569607290762
1102	19	-0.059879715601617534
1102	20	-0.8021533968956476
1102	21	0.5081086694357779
1102	22	-0.5299574883635643
1102	23	-0.05410487554917154
1102	24	0.22991439714357398
1102	25	-0.3228684706952336
1102	26	0.27160848255142866
1102	27	-0.09774345215716096
1102	28	0.1556420537323291
1102	29	0.2972095456088937
1102	30	-0.3249600067323044
1102	31	0.0760970061643036
1102	32	0.34657381586757907
1102	33	0.27528386654086745
1102	34	0.21979739102913243
1102	35	-0.04245497291609013
1102	36	0.34174649559466985
1102	37	-0.09213742815392578
1102	38	-0.016982023038465854
1102	39	1.6212681745242818
1102	40	-0.07456356189152302
1102	41	0.18563355072453191
1102	42	0.32403025561889365
1102	43	0.031619524274503874
1102	44	0.1240397187928437
1102	45	-0.3621483207366083
1102	46	-0.15889547000175952
1102	47	-0.38108595281200053
1102	48	-0.16549780234262812
1102	49	0.12397195551424289
1102	50	0.10603502355568395
1102	51	0.16511300067745793
1102	52	-0.050116255830509715
1102	53	-0.28797600284432434
1102	54	-0.0950779091581353
1102	55	-0.1971620751427352
1102	56	0.11696485881153507
1102	57	0.477818544131388
1102	58	-0.15570495340982404
1102	59	-0.5165235150934819
1102	60	-0.08641882039722049
1102	61	0.06962486544284019
1103	1	-0.05548319165426818
1103	2	-0.10140204574278044
1103	3	0.1285266649215302
1103	4	-0.7243929474416504
1103	5	0.04160677690532566
1103	6	-0.1746476961565122
1103	7	-0.011335886255423588
1103	8	0.31872784036507795
1103	9	0.2710789720420834
1103	10	-0.22331894377222739
1103	11	-0.12858315361973638
1103	12	0.18360771736357862
1103	13	0.016665061822450866
1103	14	-5.940415461793691E-4
1103	15	0.03890267692057847
1103	16	-0.6189258758749503
1103	17	-0.09956886325455372
1103	18	0.02409377348550379
1103	19	-0.24836515394854786
1103	20	-1.075298564223644
1103	21	-0.1913597740769859
1103	22	0.24099407261569894
1103	23	-0.2315099814861341
1103	24	0.14420652861022631
1103	25	0.10192751642762232
1103	26	-0.061939469833092176
1103	27	-0.06510139181426666
1103	28	-0.2268390423221689
1103	29	-0.2960673878342419
1103	30	0.17901543685532317
1103	31	-0.16476546295301375
1103	32	-0.1418292686573892
1103	33	-0.34638267534728584
1103	34	-0.133216010183709
1103	35	0.023697606255879577
1103	36	-0.07667081368136763
1103	37	-0.2286950383957418
1103	38	-0.23056291695176867
1103	39	1.099324624325881
1103	40	-0.34627128069065094
1103	41	-0.09146460005782586
1103	42	-0.07791628926393912
1103	43	-0.22223987529126532
1103	44	-0.158179342373586
1103	45	-0.47582654189405105
1103	46	-0.5871353442250111
1103	47	0.09366137068878544
1103	48	0.06955497897053675
1103	49	-0.3777275279026924
1103	50	0.09163059142271379
1103	51	0.03125060642724975
1103	52	0.2154523598766147
1103	53	-0.8032414067523916
1103	54	-0.28386281594858787
1103	55	0.46241717169413477
1103	56	0.07195475175156218
1103	57	0.15280544238698468
1103	58	0.037601926877670167
1103	59	0.19076105479496874
1103	60	0.9735796822605565
1103	61	0.374423847250128
1104	1	0.027172692576421298
1104	2	0.11104031788068625
1104	3	0.048821763169378585
1104	4	-0.7832707228548585
1104	5	0.01865153820205734
1104	6	0.1307789903725407
1104	7	0.07631396832356074
1104	8	-0.14579652652149377
1104	9	-0.18513156920783302
1104	10	0.03855237636680897
1104	11	0.37838996122739843
1104	12	0.1139758808026059
1104	13	-0.051916905972113625
1104	14	0.08331033527158382
1104	15	-0.04122784730443385
1104	16	0.037752850095117196
1104	17	0.13931884334968458
1104	18	-0.14709158510142828
1104	19	-1.0370719710018221
1104	20	-0.027955157825118704
1104	21	0.15813302580951927
1104	22	-0.01988412841998155
1104	23	-0.5582745413215591
1104	24	-0.039038133374596384
1104	25	-0.05938340078880344
1104	26	-0.006097801392996871
1104	27	-0.09262497839297623
1104	28	0.0674647102492969
1104	29	-0.07556657205783779
1104	30	-0.07491093045686625
1104	31	7.449293290306969E-4
1104	32	0.06130615022605923
1104	33	-0.33023739620988124
1104	34	-0.1267465485961381
1104	35	-0.04550860783222532
1104	36	-0.20543579913926635
1104	37	0.047108324021792214
1104	38	0.02486731643121942
1104	39	0.9684882761667026
1104	40	-0.32263504603079696
1104	41	0.06241211129919577
1104	42	0.009534941494379723
1104	43	-0.46606480258076033
1104	44	-0.5246686663432243
1104	45	0.007283339641027453
1104	46	0.0064661312662295245
1104	47	-0.21627691405264926
1104	48	-0.27999457057901367
1104	49	0.04798006945845401
1104	50	-0.3041699335116661
1104	51	-0.06777755559303955
1104	52	-0.15591743511522793
1104	53	0.012705248564344629
1104	54	0.33624075218647675
1104	55	0.00373802010159352
1104	56	0.11431837521755028
1104	57	-0.09992918200118339
1104	58	0.10981587310736529
1104	59	0.16528045608602326
1104	60	-0.17974371988174198
1104	61	0.7402321752631806
1201	1	-0.015706458399272094
1201	2	0.13232298363442396
1201	3	0.004202340597203147
1201	4	-0.4109381883347183
1201	5	-0.05980332885260253
1201	6	-0.22423641531044325
1201	7	-0.2768470495926749
1201	8	0.03265263472465563
1201	9	0.09411069942163408
1201	10	-8.747604584797841E-4
1201	11	-0.01354202553513062
1201	12	-0.17779063751304136
1201	13	-0.009950684358507544
1201	14	0.18025202190857925
1201	15	0.07967823508743521
1201	16	-0.3779153000817601
1201	17	-0.1791223297645459
1201	18	-0.435776966820022
1201	19	-0.4210443548886218
1201	20	-0.47106824694984795
1201	21	-0.05457951555759877
1201	22	-0.40653824230791713
1201	23	-0.48027613894794285
1201	24	-0.032761744010223026
1201	25	-0.06434745419517185
1201	26	-0.13173970485071607
1201	27	-0.01545391825375475
1201	28	-0.23283199179338254
1201	29	0.08930276492721134
1201	30	-0.07456858194608174
1201	31	0.10150950766978184
1201	32	-0.16820999721203123
1201	33	0.03792989809572589
1201	34	0.04639621073285838
1201	35	-0.03540932676409625
1201	36	-0.07120665386938185
1201	37	0.12353762905431154
1201	38	0.2085087634242626
1201	39	1.3205937195080182
1201	40	-0.15902098691861966
1201	41	-0.2399957398933168
1201	42	0.024198869372959564
1201	43	0.0963852715947553
1201	44	-0.3925506702231729
1201	45	-0.011431712281833484
1201	46	-0.4261482701944601
1201	47	0.0759175399014751
1201	48	0.0750778931891034
1201	49	-0.26715173471819753
1201	50	-0.05619549553380665
1201	51	0.18724355589846198
1201	52	-0.23736785163924878
1201	53	-0.20782425046567
1201	54	-0.5349791496736174
1201	55	0.019115174044308958
1201	56	-0.17910282756688645
1201	57	0.11737653521895197
1201	58	0.3915972265251426
1201	59	0.41097257065865733
1201	60	-0.5642581555772779
1201	61	-0.22704501291438106
1202	1	0.11748978131870302
1202	2	0.1040033596626273
1202	3	0.11771585411071328
1202	4	0.08641786336915658
1202	5	0.149714885013438
1202	6	0.37670365519484506
1202	7	0.3346100596905188
1202	8	0.32953865117588776
1202	9	0.4116195125826252
1202	10	0.036140515687229424
1202	11	0.20655198151540333
1202	12	0.4214455552014158
1202	13	0.22207046722785861
1202	14	-0.4654589064110439
1202	15	0.12538916340706407
1202	16	-0.03514209209116359
1202	17	0.07340410971841749
1202	18	0.04571539510712341
1202	19	0.11920241361902129
1202	20	0.0814581925331126
1202	21	0.003234820517612036
1202	22	0.2734265050481355
1202	23	0.06038191040575924
1202	24	0.09015247455617015
1202	25	0.04787346237357326
1202	26	0.20833080043718155
1202	27	-0.032668959381837226
1202	28	0.1839717684886395
1202	29	0.2985272067603546
1202	30	0.2871235449634355
1202	31	0.019018320830767266
1202	32	0.38790046107234133
1202	33	0.15091462195001526
1202	34	0.05056777360007203
1202	35	0.0453192278774992
1202	36	0.053058916048364324
1202	37	0.16049415079344698
1202	38	-0.003535889924741298
1202	39	1.1966219216231835
1202	40	0.39967466525529716
1202	41	-0.3833564919497192
1202	42	0.008570197222546483
1202	43	-0.00602365907504776
1202	44	0.30668552249127856
1202	45	-0.46501573108324123
1202	46	0.2669187098290422
1202	47	-0.03606835904094652
1202	48	0.2749603843759445
1202	49	0.40065085047569227
1202	50	0.16730626709838958
1202	51	0.46368303885968487
1202	52	-0.04400709958284921
1202	53	0.21297480946382308
1202	54	0.289110157024389
1202	55	0.3326874419644028
1202	56	-0.30642362662680966
1202	57	0.1636162531977945
1202	58	0.37273706201280277
1202	59	0.08265294668685641
1202	60	0.4870931957740723
1202	61	0.752802225628507
1203	1	-0.3241227809642453
1203	2	0.1445891968965043
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2403	20	0.04578829803968887
2403	21	0.020751802740477387
2403	22	0.2532501789894539
2403	23	-0.03072952188214373
2403	24	0.06465746082586321
2403	25	-0.009360241918955126
2403	26	0.08611660763475015
2403	27	0.08318593294364263
2403	28	-0.10809355887080585
2403	29	-0.4127316456749739
2403	30	0.2930203508602389
2403	31	-0.23957396129092245
2403	32	0.017037619621262934
2403	33	-0.033158220946361894
2403	34	0.03738667806603502
2403	35	0.03306312150374424
2403	36	-0.3548034918140459
2403	37	-0.08763419145254403
2403	38	-0.13205157138159507
2403	39	1.3055972070690558
2403	40	0.20502225883818426
2403	41	-0.13071882754734787
2403	42	-0.12913756401462706
2403	43	0.2951181244196732
2403	44	0.1426155701860381
2403	45	0.16108630678350266
2403	46	0.5185157614260945
2403	47	0.05371340132904834
2403	48	0.026600794839886532
2403	49	-0.03918149523900638
2403	50	-0.19500042462006206
2403	51	0.1306869411577054
2403	52	0.17608250874205567
2403	53	0.05711413595609116
2403	54	-0.29236115385868544
2403	55	-0.04488157089873113
2403	56	0.330720469340811
2403	57	-0.32292804511120465
2403	58	0.30353631045910845
2403	59	0.6664945422931581
2403	60	0.4063300797168381
2403	61	0.27464064158456836
2404	1	0.16907615029637668
2404	2	0.48014907059094725
2404	3	-0.23492809418540617
2404	4	0.4912357247087584
2404	5	0.03802275810392075
2404	6	0.28663314990694744
2404	7	0.17967468953776233
2404	8	-0.02169519136230491
2404	9	0.09411069942163408
2404	10	0.30347306562848075
2404	11	-0.057020286404693366
2404	12	0.2896006668347866
2404	13	0.14222322868496917
2404	14	0.12590419582161871
2404	15	0.11228693073961438
2404	16	0.3720846999182399
2404	17	0.0056602789311099855
2404	18	0.0859621636147665
2404	19	-0.018870441845137975
2404	20	0.49632305739798
2404	21	0.03237700618153383
2404	22	0.2238965403007782
2404	23	0.3892890784433618
2404	24	-0.02189217879283234
2404	25	0.0660873284135235
2404	26	0.031303773410151337
2404	27	0.21280695131146388
2404	28	0.1476027908153128
2404	29	0.1979984171011253
2404	30	0.1428227224017391
2404	31	0.25368342071325856
2404	32	0.07092043757057809
2404	33	0.00532120244355383
2404	34	0.1116136020372025
2404	35	0.31241676019241993
2404	36	-0.016858827782421315
2404	37	0.27571154209779536
2404	38	0.3063348503807859
2404	39	1.614071980377588
2404	40	0.1344572739509431
2404	41	0.4556564340197298
2404	42	0.5459379998077409
2404	43	0.6181244020295367
2404	44	0.5096232428203038
2404	45	0.7494378529355572
2404	46	0.6281995558925004
2404	47	0.45635232251017044
2404	48	0.4229039801456267
2404	49	0.6132830478904978
2404	50	0.5525001566401073
2404	51	0.1655044254636806
2404	52	0.39306693096944656
2404	53	0.8573931408386812
2404	54	0.9432817198916013
2404	55	0.7147673479573484
2404	56	0.5491580419983322
2404	57	1.0304200134798194
2404	58	0.6524667917425333
2404	59	0.9979290923977899
2404	60	0.4357418444227221
2404	61	1.088172378389963
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	8.0
18	15.0
19	31.0
20	48.0
21	68.0
22	78.0
23	123.0
24	175.0
25	217.0
26	364.0
27	411.0
28	523.0
29	632.0
30	724.0
31	878.0
32	1166.0
33	1321.0
34	1559.0
35	1988.0
36	2948.0
37	4318.0
38	13036.0
39	60651.0
40	26268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.90557209698001	52.37856231390897	28.72564866014462	8.990216928966397
2	53.5983865340266	9.224668328922398	10.299461327024705	26.8774838100263
3	9.77626541897065	52.67290514674606	27.8860059549128	9.664823479370481
4	9.96852403232667	9.390046788600594	53.51850276478094	27.122926414291793
5	10.208421948107189	9.094853253934495	27.50829434283284	53.18843045512548
6	53.81029349213101	9.922586133560188	9.71331348362399	26.553806890684818
7	54.41173968524032	9.467460655040409	27.400255210548703	8.720544449170566
8	8.363249680986815	60.218630370055294	6.022118247554233	25.39600170140366
9	15.775414717141642	52.04593789876648	25.26839642705232	6.910250957039557
10	51.206295193534665	16.02381965121225	24.929817099106764	7.840068056146321
11	7.991492981709911	50.25350914504466	33.12888132709485	8.626116546150573
12	53.03360272224585	7.941301573798383	24.86771586558911	14.157379838366651
13	17.030199914929817	8.361548277328797	4.957039557635049	69.65121225010634
14	15.786473840918758	52.45087196937474	5.62569119523607	26.136962994470437
15	8.629519353466609	16.291790727350065	24.741811994895787	50.336877924287535
16	9.413015737983836	58.610803913228416	6.8056146320714594	25.170565716716293
17	15.361122926414291	9.851977881752445	6.185452998723947	68.60144619310931
18	51.59251382390472	14.889834113143344	25.357720119098254	8.15993194385368
19	14.94853253934496	8.471288813270949	49.56699276903445	27.01318587834964
20	10.49425776265419	10.516376010208422	7.766056997022544	71.22330923011484
21	12.837090599744789	11.932794555508295	67.38153977031051	7.84857507443641
22	55.65291365376436	8.970650786899192	8.715440238196512	26.66099532113994
23	10.887282007656317	55.475967673330494	6.801361122926414	26.835389196086773
24	29.65631646108039	51.80093577201191	10.700127605274352	7.842620161633347
25	11.464908549553382	10.483198638877074	25.390046788600596	52.66184602296895
26	14.341982135261592	11.370480646533391	66.98426201616333	7.303275202041684
27	32.70267971076138	10.900042535091451	6.148022118247554	50.249255635899615
28	10.80391322841344	15.094002552105486	23.606975754997872	50.495108464483195
29	11.692045937898767	56.03658017864739	23.68694172692471	8.584432156529138
30	72.80136112292641	12.109740535942153	6.169289663972777	8.919608677158655
31	9.357720119098257	15.629094002552105	66.1709910676308	8.842194810718842
32	12.913653764355592	11.43428328370906	24.432156529136538	51.219906422798815
33	26.921310080816674	52.58528285835814	7.557635048915355	12.935772011909826
34	9.385793279455552	14.219481071884305	25.09059974478945	51.30412590387069
35	51.89706507868992	9.91237771161208	25.498085920884726	12.69247128881327
36	25.79157805189281	14.843896214376862	49.55082943428329	9.813696299447045
37	10.603998298596341	27.13823904721395	7.246278179498086	55.01148447469162
38	29.58570820927265	11.709910676307954	49.32624415142492	9.378136962994471
39	52.21057809713719	27.54152794736426	9.911366052055975	10.336527903442581
40	24.98000850701829	12.586984262016163	7.006380263717567	55.426626967247984
41	10.001701403658018	69.46235644406636	10.262016163334751	10.273925988940876
42	28.189706507868994	54.23564440663547	6.586984262016163	10.98766482347937
43	8.269672479795831	71.9710761378137	8.912803062526585	10.846448319863889
44	8.428753721820502	15.151850276478093	64.91790727350065	11.501488728200766
45	9.4666099532114	25.710761378136965	8.550404083368779	56.272224585282856
46	24.71118672905147	11.068481497235219	52.524883028498515	11.695448745214803
47	50.90089323692047	25.014036580178644	8.949383241173967	15.135686941726926
48	50.04763930242449	10.467886005954913	23.64100382815823	15.843470863462356
49	51.877498936622715	12.831986388770735	7.978732454274777	27.311782220331775
50	71.97532964695874	9.031901318587835	7.836665248830285	11.15610378562314
51	66.48490004253509	10.947681837515951	11.631646108039133	10.935772011909824
52	51.395150999574646	26.468736707783926	8.095278604849	14.040833687792428
53	51.92513823904722	28.153126329221607	8.993619736282433	10.928115695448746
54	66.9136537643556	11.420672054444916	8.12079965971927	13.54487452148022
55	55.27860484900042	25.98979157805189	8.095278604849	10.636324968098682
56	66.44746916205871	11.324542747766909	10.723947256486602	11.504040833687792
57	50.85665674181199	11.572947681837515	7.516801361122926	30.053594215227562
58	51.62994470438112	26.307954062101235	10.968949383241174	11.093151850276477
59	55.079540621012335	10.746916205869843	8.22373458102935	25.949808592088473
60	52.33943002977456	24.736707783921734	7.907273500638026	15.016588685665674
61	54.777541471714166	11.899617184176945	8.720544449170566	24.602296894938323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	1.0
14	1.5
15	2.0
16	1.5
17	1.5
18	2.0
19	11.5
20	16.5
21	12.0
22	17.0
23	22.0
24	25.0
25	36.0
26	44.0
27	66.0
28	88.0
29	84.0
30	104.0
31	128.0
32	149.0
33	170.0
34	179.0
35	243.5
36	299.0
37	343.5
38	388.0
39	407.0
40	543.0
41	660.0
42	720.0
43	997.5
44	1215.0
45	1774.0
46	2333.0
47	11816.5
48	14065.0
49	6830.0
50	7068.0
51	7306.0
52	7471.5
53	6868.5
54	6100.0
55	5059.5
56	4019.0
57	3370.0
58	2363.0
59	2005.0
60	3299.5
61	25055.5
62	45517.0
63	23343.0
64	1169.0
65	771.5
66	351.0
67	328.0
68	269.0
69	210.0
70	215.5
71	162.0
72	103.0
73	98.5
74	94.0
75	70.0
76	37.0
77	28.0
78	27.0
79	26.0
80	17.5
81	7.5
82	6.0
83	8.0
84	7.5
85	5.0
86	2.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	1.0
95	2.0
96	1.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03317737133134836
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1569544874521482
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	117550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.400255210548703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9251451457667	21.62569119523607
2	13.741500822751403	7.53041259038707
3	3.4400322891117394	2.827732879625691
4	1.0928622434723214	1.1977881752445767
5	0.5433263994535689	0.7443641003828159
6	0.3011580614114068	0.4951084644831986
7	0.2514825048899376	0.48234793704806467
8	0.1955975038032848	0.4287537218205019
9	0.17386444782514204	0.4287537218205019
>10	1.1673755782545252	6.436410038281583
>50	0.09935111304293831	1.7720119098256062
>100	0.0558850010866528	2.641429179072735
>500	0.003104722282591822	0.46278179498085925
>1k	0.003104722282591822	1.2411739685240322
>5k	0.0	0.0
>10k+	0.006209444565183644	51.68524032326669
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAATCTGGGGGGGGGGGGGGG	44258	37.65036154827733	TruSeq Adapter, Index 18 (97% over 40bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC	16498	14.034878774989364	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTNCAAATCTGGGGGGGGGGGGGGG	1459	1.2411739685240322	TruSeq Adapter, Index 11 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGNGAGATAGATCGGAAGAGCACAC	544	0.46278179498085925	No Hit
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATCGGAAGAGCACACGTCT	486	0.4134410888983411	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTAGAGAGATAGATCGGAAGAGCACACG	340	0.289238621863037	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAATCTGGGGGGGGGGGGGGT	237	0.20161633347511695	TruSeq Adapter, Index 18 (97% over 40bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAAGAGATAGATCGGAAGAGCACACG	211	0.17949808592088473	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAATCTGGGGGGGGGGGGGTG	181	0.15397703105061677	TruSeq Adapter, Index 18 (97% over 40bp)
CTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACGTC	157	0.13356018715440238	No Hit
AGATCGGAAGAGCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAAT	157	0.13356018715440238	TruSeq Adapter, Index 18 (97% over 40bp)
CTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACG	150	0.12760527435133986	No Hit
AGATCGGAAGAGCACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAATC	144	0.12250106337728627	TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAATCTGGG	128	0.10888983411314335	TruSeq Adapter, Index 18 (97% over 40bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACGTC	126	0.10718843045512548	No Hit
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGGAGAGATAGATCGGAAGAGCACACG	118	0.10038281582305403	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	42.555508294342836	0.0	0.0	0.0	0.0
2	42.74351339855381	0.0	0.0	0.0	0.0
3	42.85410463632497	0.0	0.0	0.0	0.0
4	43.00212675457252	0.0	0.0	0.0	0.0
5	43.20289238621863	0.0	0.0	0.0	0.0
6	43.552530837941305	0.0	0.0	0.0	0.0
7	44.027222458528286	0.0	0.0	0.0	0.0
8	48.991067630795406	0.0	0.0	0.0	0.0
9	49.535516801361126	0.0	0.0	0.0	0.0
10	49.994895789025946	0.0	0.0	0.0	0.0
11	50.31731178222033	0.0	0.0	0.0	0.0
12	50.928966397277755	0.0	0.0	0.0	0.0
13	51.584006805614635	0.0	0.0	0.0	0.0
14	52.26371756699277	0.0	0.0	0.0	0.0
15	53.20884729902169	0.0	0.0	0.0	0.0
16	54.2764780944279	0.0	0.0	0.0	0.0
17	55.064227988090174	0.0	0.0	0.0	0.0
18	55.48192258613356	0.0	0.0	0.0	0.0
19	56.15652913653764	0.0	0.0	0.0	0.0
20	56.53764355593365	0.0	0.0	0.0	0.0
21	60.0569970225436	0.0	0.0	0.0	0.0
22	60.369204593789874	0.0	0.0	0.0	0.0
23	60.65759251382391	0.0	0.0	0.0	0.0
24	60.984262016163335	0.0	0.0	0.0	0.0
25	61.269247128881325	0.0	0.0	0.0	0.0
26	61.607826456826885	0.0	0.0	0.0	0.0
27	61.89876648234794	0.0	0.0	0.0	0.0
28	62.18034878774989	0.0	0.0	0.0	0.0
29	62.507868991918336	0.0	0.0	0.0	0.0
30	62.88472990216929	0.0	0.0	0.0	0.0
31	63.25138239047214	0.0	0.0	0.0	0.0
32	63.74053594215228	0.0	0.0	0.0	0.0
33	64.2475542322416	0.0	0.0	0.0	0.0
34	64.68821777966822	0.0	0.0	0.0	0.0
35	65.04381114419397	0.0	0.0	0.0	0.0
36	65.37388345384943	0.0	0.0	0.0	0.0
37	65.67077839217355	0.0	0.0	0.0	0.0
38	65.90982560612505	0.0	0.0	0.0	0.0
39	66.18290089323692	0.0	0.0	0.0	0.0
40	66.41599319438536	0.0	0.0	0.0	0.0
41	67.1705657167163	0.0	0.0	0.0	0.0
42	67.63504891535517	0.0	0.0	0.0	0.0
43	67.99574649085496	0.0	0.0	0.0	0.0
44	69.51765206295194	0.0	0.0	0.0	0.0
45	85.28881327094854	0.0	0.0	0.0	0.0
46	85.76775840068056	0.0	0.0	0.0	0.0
47	85.99149298170991	0.0	0.0	0.0	0.0
48	86.21267545725223	0.0	0.0	0.0	0.0
49	86.41599319438536	0.0	0.0	0.0	0.0
50	86.57337303275202	0.0	0.0	0.0	0.0
>>END_MODULE