FastQCFastQC Report
Sat 10 Aug 2024
AM-AL-AB_S54_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-AB_S54_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117550
Sequences flagged as poor quality0
Sequence length61
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAATCTGGGGGGGGGGGGGGG4425837.65036154827733TruSeq Adapter, Index 18 (97% over 40bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACAC1649814.034878774989364No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTNCAAATCTGGGGGGGGGGGGGGG14591.2411739685240322TruSeq Adapter, Index 11 (97% over 37bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGNGAGATAGATCGGAAGAGCACAC5440.46278179498085925No Hit
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATCGGAAGAGCACACGTCT4860.4134410888983411Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTAGAGAGATAGATCGGAAGAGCACACG3400.289238621863037No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAATCTGGGGGGGGGGGGGGT2370.20161633347511695TruSeq Adapter, Index 18 (97% over 40bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAAGAGATAGATCGGAAGAGCACACG2110.17949808592088473No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAATCTGGGGGGGGGGGGGTG1810.15397703105061677TruSeq Adapter, Index 18 (97% over 40bp)
CTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACGTC1570.13356018715440238No Hit
AGATCGGAAGAGCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAAT1570.13356018715440238TruSeq Adapter, Index 18 (97% over 40bp)
CTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACG1500.12760527435133986No Hit
AGATCGGAAGAGCACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAATC1440.12250106337728627TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAATCTGGG1280.10888983411314335TruSeq Adapter, Index 18 (97% over 40bp)
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGAGAGAGATAGATCGGAAGAGCACACGTC1260.10718843045512548No Hit
TCTCTCTCTTTTCCTCCTCCTCCGTTGTTGTTGTTGGAGAGATAGATCGGAAGAGCACACG1180.10038281582305403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)