##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-AB.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9525 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.04377952755905 40.0 40.0 40.0 40.0 40.0 2 38.82141732283465 40.0 40.0 40.0 40.0 40.0 3 38.916115485564305 40.0 40.0 40.0 40.0 40.0 4 38.57511811023622 40.0 40.0 40.0 40.0 40.0 5 38.92199475065617 40.0 40.0 40.0 40.0 40.0 6 39.09711286089239 40.0 40.0 40.0 40.0 40.0 7 39.18362204724409 40.0 40.0 40.0 40.0 40.0 8 39.040419947506564 40.0 40.0 40.0 40.0 40.0 9 39.20755905511811 40.0 40.0 40.0 40.0 40.0 10-11 39.140577427821526 40.0 40.0 40.0 40.0 40.0 12-13 39.114960629921256 40.0 40.0 40.0 40.0 40.0 14-15 39.25816272965879 40.0 40.0 40.0 40.0 40.0 16-17 39.2854593175853 40.0 40.0 40.0 40.0 40.0 18-19 39.26194225721785 40.0 40.0 40.0 40.0 40.0 20-21 39.20167979002625 40.0 40.0 40.0 40.0 40.0 22-23 39.20377952755905 40.0 40.0 40.0 40.0 40.0 24-25 39.26719160104987 40.0 40.0 40.0 40.0 40.0 26-27 38.98897637795275 40.0 40.0 40.0 40.0 40.0 28-29 38.991916010498684 40.0 40.0 40.0 40.0 40.0 30-31 39.08058414248313 40.0 40.0 40.0 40.0 40.0 32-33 39.12772607755849 40.0 40.0 40.0 40.0 40.0 34-35 39.159430802340864 40.0 40.0 40.0 40.0 40.0 36-37 39.12326229682323 40.0 40.0 40.0 40.0 40.0 38-39 38.899087211767544 40.0 40.0 40.0 40.0 40.0 40-41 39.04339188283366 40.0 40.0 40.0 40.0 40.0 42-43 39.03959932506164 40.0 40.0 40.0 40.0 40.0 44-45 38.83005856377394 40.0 40.0 40.0 40.0 40.0 46-47 38.67966987532765 40.0 40.0 40.0 40.0 40.0 48-49 38.78478759127147 40.0 40.0 40.0 40.0 40.0 50-51 38.82519686331124 40.0 40.0 40.0 40.0 40.0 52-53 38.61406586557966 40.0 40.0 40.0 40.0 40.0 54-55 38.57123676524126 40.0 40.0 40.0 40.0 40.0 56-57 38.5041447698914 40.0 40.0 40.0 40.0 40.0 58-59 38.4301400155008 40.0 40.0 40.0 40.0 40.0 60-61 38.394313629102435 40.0 40.0 40.0 40.0 40.0 62-63 39.15406819493293 40.0 40.0 40.0 40.0 40.0 64-65 39.082600523227555 40.0 40.0 40.0 40.0 40.0 66-67 39.14364212367021 40.0 40.0 40.0 40.0 40.0 68-69 39.07488026198915 40.0 40.0 40.0 40.0 40.0 70-71 39.0758691643518 40.0 40.0 40.0 40.0 40.0 72-73 39.12363875983252 40.0 40.0 40.0 40.0 40.0 74-75 39.12677387094993 40.0 40.0 40.0 40.0 40.0 76-77 39.09926171232465 40.0 40.0 40.0 40.0 40.0 78-79 39.106860985436526 40.0 40.0 40.0 40.0 40.0 80-81 39.097803302780676 40.0 40.0 40.0 40.0 40.0 82-83 38.92167606342147 40.0 40.0 40.0 40.0 40.0 84-85 38.740786433953815 40.0 40.0 40.0 40.0 40.0 86-87 38.949863684672614 40.0 40.0 40.0 40.0 40.0 88-89 38.713364928909954 40.0 40.0 40.0 40.0 40.0 90-91 38.29904761904761 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.4430152305237556 1101 2 -0.7611609639298251 1101 3 -0.31551933989640446 1101 4 -1.8133304030789716 1101 5 -1.1438970178278893 1101 6 -2.957349282137102 1101 7 -0.9118255271471725 1101 8 -0.4196765329003611 1101 9 -0.5532661741672626 1101 10-11 0.3250817843367102 1101 12-13 0.8704996279865043 1101 14-15 -0.2867718047408303 1101 16-17 0.0333885696340559 1101 18-19 -0.7320573965764581 1101 20-21 -0.5350140084662698 1101 22-23 -0.375833483102312 1101 24-25 0.18899273920678894 1101 26-27 -0.019343560836702522 1101 28-29 0.10855036415284758 1101 30-31 0.6343237343844805 1101 32-33 -1.0097006759332388 1101 34-35 0.7544535233913763 1101 36-37 0.8774264115522783 1101 38-39 0.28394404464257406 1101 40-41 0.1564467907482694 1101 42-43 0.08762061162344992 1101 44-45 0.8977969142701454 1101 46-47 -0.16915637763668911 1101 48-49 -1.0752258099442713 1101 50-51 -0.13977923879239285 1101 52-53 0.25399982929008047 1101 54-55 0.036245004995002716 1101 56-57 -3.1361228844684774 1101 58-59 0.10033540805598307 1101 60-61 -0.1484421624127492 1101 62-63 -2.2624343487395038 1101 64-65 -3.512952672327671 1101 66-67 -8.592931547619045 1101 68-69 -4.387028769841272 1101 70-71 -0.9247519841269849 1101 72-73 -1.0619791666666671 1101 74-75 -2.5416666666666643 1101 76-77 0.5107142857142861 1101 78-79 4.438095238095229 1101 80-81 4.041666666666664 1101 82-83 9.0625 1101 84-85 -27.947916666666664 1101 86-87 -21.875 1101 88-89 -19.625 1101 90-91 -8.75 1102 1 0.24962546211693848 1102 2 -1.8087800115488761 1102 3 0.19530317092610971 1102 4 -0.06441265416122377 1102 5 0.09419822026735147 1102 6 -2.3859207107085325 1102 7 -0.2191848345064784 1102 8 0.8530507398269123 1102 9 0.1393745184734314 1102 10-11 0.30776576702069036 1102 12-13 0.20383296131983286 1102 14-15 -1.0962956142646405 1102 16-17 -0.37786684162135487 1102 18-19 -0.4203690848881436 1102 20-21 0.5558950824428237 1102 22-23 0.3341232268544019 1102 24-25 -0.9711804209663768 1102 26-27 -0.35700589849903963 1102 28-29 0.060931316533796576 1102 30-31 -1.0972779972172546 1102 32-33 -0.22874829498086058 1102 34-35 -0.9297570029244184 1102 36-37 -2.1752051673950916 1102 38-39 -1.0820954885796255 1102 40-41 -1.366280481979004 1102 42-43 -0.39849049948766435 1102 44-45 -0.5688697523965232 1102 46-47 -0.8358230443033605 1102 48-49 -0.19643793115639596 1102 50-51 1.1935540945409358 1102 52-53 1.587333162623409 1102 54-55 1.6362450049950041 1102 56-57 0.463877115531524 1102 58-59 0.8114465191670988 1102 60-61 1.4515578375872522 1102 62-63 -0.20529149159663973 1102 64-65 0.8870473276723274 1102 66-67 1.007068452380956 1102 68-69 1.1129712301587276 1102 70-71 2.575248015873015 1102 72-73 2.938020833333333 1102 74-75 2.7916666666666714 1102 76-77 1.8440476190476147 1102 78-79 4.438095238095229 1102 80-81 4.041666666666664 1102 82-83 9.0625 1102 84-85 12.052083333333336 1102 86-87 14.125 1102 88-89 12.375 1102 90-91 -8.75 1103 1 1.0115302240216977 1103 2 -0.5944942972631608 1103 3 0.9572079328308689 1103 4 0.29273020298163743 1103 5 0.8561029821721107 1103 6 1.042650717862898 1103 7 0.5427199273982808 1103 8 0.18638407316024797 1103 9 0.2346126137115263 1103 10-11 -0.31128185202693004 1103 12-13 -0.29616703868016003 1103 14-15 -0.5367718047408303 1103 16-17 -0.24691446066896816 1103 18-19 0.05582139130233088 1103 20-21 -0.02743825089050489 1103 22-23 0.3817422744734529 1103 24-25 -0.030704230490179896 1103 26-27 0.40489886340572667 1103 28-29 -2.974782969180481 1103 30-31 -1.168706568645824 1103 32-33 0.8712517050191408 1103 34-35 0.7544535233913763 1103 36-37 0.2620417961676651 1103 38-39 0.8993286600271873 1103 40-41 -0.38900775470627735 1103 42-43 0.9765095005123356 1103 44-45 0.8977969142701454 1103 46-47 1.1641769556966395 1103 48-49 1.2581075233890573 1103 50-51 0.19355409454093575 1103 52-53 1.587333162623409 1103 54-55 0.6362450049950041 1103 56-57 0.26387711553152116 1103 58-59 1.7003354080559845 1103 60-61 1.4515578375872522 1103 62-63 0.9375656512604991 1103 64-65 0.8870473276723274 1103 66-67 1.007068452380956 1103 68-69 1.1129712301587276 1103 70-71 2.575248015873015 1103 72-73 2.938020833333333 1103 74-75 2.7916666666666714 1103 76-77 4.510714285714286 1103 78-79 4.438095238095229 1103 80-81 4.041666666666664 1103 82-83 9.0625 1103 84-85 12.052083333333336 1103 86-87 18.125 1103 88-89 -19.625 1103 90-91 -8.75 1104 1 1.0115302240216977 1104 2 0.20180199903313678 1104 3 0.3646153402382737 1104 4 0.2742116844631184 1104 5 0.8561029821721107 1104 6 -0.14253446732228525 1104 7 0.5427199273982808 1104 8 -0.3321344453582711 1104 9 0.3086866877855954 1104 10-11 0.39242185167677235 1104 12-13 0.5742033316902067 1104 14-15 0.4169318989628721 1104 16-17 0.3734559097013985 1104 18-19 0.7224880579689952 1104 20-21 -0.03669751014977152 1104 22-23 0.41877931151048386 1104 24-25 0.5526291028431487 1104 26-27 0.7752692337760934 1104 28-29 0.5159577715602524 1104 30-31 0.40536750542824507 1104 32-33 0.20458503835246944 1104 34-35 -0.6205464766086237 1104 36-37 -0.1940021598762911 1104 38-39 0.28394404464257406 1104 40-41 0.21705285135432462 1104 42-43 0.1765095005123385 1104 44-45 0.8977969142701454 1104 46-47 1.1641769556966395 1104 48-49 -0.4918924766109427 1104 50-51 -2.139779238792393 1104 52-53 -0.012666837376592355 1104 54-55 -1.8637549950049959 1104 56-57 -0.7361228844684788 1104 58-59 -3.7996645919440155 1104 60-61 1.4515578375872522 1104 62-63 0.9375656512604991 1104 64-65 0.8870473276723274 1104 66-67 1.007068452380956 1104 68-69 1.1129712301587276 1104 70-71 -37.424751984126985 1104 72-73 -37.06197916666667 1104 74-75 -37.20833333333333 1104 76-77 -35.489285714285714 1104 78-79 -35.56190476190477 1104 80-81 -35.958333333333336 1104 82-83 -30.9375 1104 84-85 -27.947916666666664 1104 86-87 -21.875 1104 88-89 -19.625 1104 90-91 -8.75 1201 1 -0.321803109311638 1201 2 -0.7611609639298251 1201 3 -1.3761254005024668 1201 4 -1.7072697970183626 1201 5 -1.1438970178278893 1201 6 0.3759840511962338 1201 7 0.5427199273982808 1201 8 -1.6469492601730877 1201 9 -0.2653873862884737 1201 10-11 0.35538481463974136 1201 12-13 0.5371662946531757 1201 14-15 0.04656152859249829 1201 16-17 0.33641887266436754 1201 18-19 0.055821391302323775 1201 20-21 -0.11077158422384059 1201 22-23 0.3817422744734529 1201 24-25 0.2192957695098201 1201 26-27 0.07156553007239097 1201 28-29 -1.1414496358471524 1201 30-31 0.9979600980208403 1201 32-33 0.08656270980382885 1201 34-35 -1.2455464766086237 1201 36-37 0.3059978401237089 1201 38-39 0.23266199336051585 1201 40-41 0.883719518020996 1201 42-43 -0.4780359540331176 1201 44-45 -0.6294758130025855 1201 46-47 0.3641769556966423 1201 48-49 1.2581075233890573 1201 50-51 0.3935540945409386 1201 52-53 1.587333162623409 1201 54-55 0.6362450049950041 1201 56-57 -1.0694562178018074 1201 58-59 1.7003354080559845 1201 60-61 1.4515578375872522 1201 62-63 0.9375656512604991 1201 64-65 0.8870473276723274 1201 66-67 1.007068452380956 1201 68-69 1.1129712301587276 1201 70-71 -5.424751984126985 1201 72-73 2.938020833333333 1201 74-75 2.7916666666666714 1201 76-77 -35.489285714285714 1201 78-79 -35.56190476190477 1201 80-81 -35.958333333333336 1201 82-83 -30.9375 1201 84-85 -27.947916666666664 1201 86-87 -21.875 1201 88-89 -19.625 1201 90-91 -8.75 1202 1 1.0115302240216977 1202 2 0.5721723694035106 1202 3 -0.20945873383579539 1202 4 -1.0406031303516983 1202 5 0.18943631550544637 1202 6 0.3759840511962338 1202 7 -0.12394673926838351 1202 8 0.18638407316024797 1202 9 0.2346126137115263 1202 10-11 0.022051481306405663 1202 12-13 0.28716629465317567 1202 14-15 0.3798948619258411 1202 16-17 0.33641887266436754 1202 18-19 0.055821391302323775 1202 20-21 0.2225617491094951 1202 22-23 0.7150756078067815 1202 24-25 -0.1140375638235156 1202 26-27 -0.42843446992760903 1202 28-29 -1.3081163025138167 1202 30-31 0.4146267646875117 1202 32-33 0.06317089693833822 1202 34-35 0.18302495196280688 1202 36-37 0.8774264115522783 1202 38-39 0.8993286600271873 1202 40-41 0.883719518020996 1202 42-43 0.9765095005123356 1202 44-45 -0.2450602285869934 1202 46-47 0.021319812839500685 1202 48-49 1.2581075233890573 1202 50-51 -0.13977923879239285 1202 52-53 -1.0793335040432552 1202 54-55 1.6362450049950041 1202 56-57 1.2638771155315212 1202 58-59 0.10033540805598307 1202 60-61 1.4515578375872522 1202 62-63 0.9375656512604991 1202 64-65 0.8870473276723274 1202 66-67 1.007068452380956 1202 68-69 1.1129712301587276 1202 70-71 2.575248015873015 1202 72-73 2.938020833333333 1202 74-75 2.7916666666666714 1202 76-77 4.510714285714286 1202 78-79 4.438095238095229 1202 80-81 4.041666666666664 1202 82-83 -30.9375 1202 84-85 -27.947916666666664 1202 86-87 -21.875 1202 88-89 -19.625 1202 90-91 -8.75 1203 1 0.3448635573550334 1203 2 -0.09449429726316083 1203 3 0.2905412661642046 1203 4 0.7927302029816374 1203 5 0.8561029821721107 1203 6 0.3759840511962338 1203 7 0.5427199273982808 1203 8 -0.48028259350642344 1203 9 0.2346126137115263 1203 10-11 0.35538481463974136 1203 12-13 -0.79616703868016 1203 14-15 0.3798948619258411 1203 16-17 0.33641887266436754 1203 18-19 0.3891547246356666 1203 20-21 0.5558950824428237 1203 22-23 -0.2849243921932185 1203 24-25 0.5526291028431487 1203 26-27 -0.7617678032609376 1203 28-29 0.525217030819519 1203 30-31 -1.2520399019791597 1203 32-33 0.47125170501914226 1203 34-35 0.3100090789469334 1203 36-37 0.8774264115522783 1203 38-39 0.8993286600271873 1203 40-41 0.883719518020996 1203 42-43 -0.02349049948766435 1203 44-45 0.8977969142701454 1203 46-47 1.1641769556966395 1203 48-49 -1.0752258099442713 1203 50-51 -0.13977923879239285 1203 52-53 1.587333162623409 1203 54-55 1.6362450049950041 1203 56-57 1.2638771155315212 1203 58-59 1.7003354080559845 1203 60-61 1.4515578375872522 1203 62-63 0.9375656512604991 1203 64-65 -4.446286005661008 1203 66-67 1.007068452380956 1203 68-69 -5.887028769841272 1203 70-71 -11.424751984126985 1203 72-73 -1.0619791666666671 1203 74-75 2.7916666666666714 1203 76-77 4.510714285714286 1203 78-79 4.438095238095229 1203 80-81 4.041666666666664 1203 82-83 -30.9375 1203 84-85 -27.947916666666664 1203 86-87 -21.875 1203 88-89 -19.625 1203 90-91 -8.75 1204 1 -0.06539285290138253 1204 2 0.6234544206855617 1204 3 -0.11971514409221129 1204 4 -0.6944492841978516 1204 5 0.8561029821721107 1204 6 1.042650717862898 1204 7 0.5427199273982808 1204 8 -0.37771849094232124 1204 9 -0.1756437965448896 1204 10-11 0.38102584028076336 1204 12-13 0.5628073202941977 1204 14-15 0.1747666567976296 1204 16-17 -0.25332471707922366 1204 18-19 0.4147957502766886 1204 20-21 0.2482027747505171 1204 22-23 -0.20800131527013832 1204 24-25 0.5526291028431487 1204 26-27 -0.928434469927609 1204 28-29 0.2623965179990009 1204 30-31 0.3825754826362271 1204 32-33 -0.09303400926656735 1204 34-35 -0.08765173976651397 1204 36-37 0.2107597448856069 1204 38-39 0.8993286600271873 1204 40-41 0.883719518020996 1204 42-43 -1.0234904994876644 1204 44-45 -1.1022030857298546 1204 46-47 -2.302489710970029 1204 48-49 -1.853003587722057 1204 50-51 -0.5842236832368428 1204 52-53 -0.8571112818210338 1204 54-55 -0.14153277278277443 1204 56-57 1.2638771155315212 1204 58-59 0.3670020747226559 1204 60-61 -4.548442162412748 1204 62-63 0.9375656512604991 1204 64-65 0.8870473276723274 1204 66-67 -2.992931547619044 1204 68-69 1.1129712301587276 1204 70-71 2.575248015873015 1204 72-73 2.938020833333333 1204 74-75 2.7916666666666714 1204 76-77 4.510714285714286 1204 78-79 4.438095238095229 1204 80-81 4.041666666666664 1204 82-83 9.0625 1204 84-85 12.052083333333336 1204 86-87 18.125 1204 88-89 20.375 1204 90-91 31.25 1301 1 -1.296162083670609 1301 2 0.008069805300941368 1301 3 0.9572079328308689 1301 4 1.4593968696483017 1301 5 -0.22082009475096953 1301 6 -0.8035031282909486 1301 7 -0.6880493033709527 1301 8 -1.4546415678653943 1301 9 0.9012792803781906 1301 10-11 0.07333353258845676 1301 12-13 -0.05257729509041553 1301 14-15 0.7132281952591697 1301 16-17 -0.7917862555407709 1301 18-19 -0.5082811728002312 1301 20-21 -0.5979510714033296 1301 22-23 -0.43877054603937893 1301 24-25 -0.293524743310698 1301 26-27 -0.6976652391583826 1301 28-29 -0.04529578969330572 1301 30-31 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2403 44-45 -0.5022030857298532 2403 46-47 -0.4790978981045342 2403 48-49 -0.5065983589638847 2403 50-51 -0.8064459054590642 2403 52-53 1.587333162623409 2403 54-55 0.702911671661667 2403 56-57 0.19721044886485117 2403 58-59 1.167002074722653 2403 60-61 0.2848911709205808 2403 62-63 -1.062434348739501 2403 64-65 -0.4462860056610083 2403 66-67 -1.6595982142857082 2403 68-69 -1.7759176587301582 2403 70-71 -1.424751984126985 2403 72-73 1.1880208333333329 2403 74-75 2.7916666666666714 2403 76-77 3.3678571428571473 2403 78-79 2.1523809523809447 2403 80-81 1.375 2403 82-83 3.0625 2403 84-85 5.052083333333336 2403 86-87 18.125 2403 88-89 20.375 2403 90-91 31.25 2404 1 -0.23846977597830232 2404 2 0.23883903607017487 2404 3 -0.6261254005024668 2404 4 0.45939686964830173 2404 5 -0.06056368449455363 2404 6 0.1259840511962338 2404 7 0.2093865940649451 2404 8 -1.2302825935064234 2404 9 -0.5987207196218094 2404 10-11 0.022051481306398557 2404 12-13 0.03716629465316856 2404 14-15 0.04656152859249829 2404 16-17 0.5030855393310247 2404 18-19 0.22248805796899518 2404 20-21 -0.2357715842238406 2404 22-23 -0.07659105885988282 2404 24-25 0.2609624361764773 2404 26-27 0.07156553007239097 2404 28-29 0.6085503641528476 2404 30-31 0.8312934313541689 2404 32-33 0.3213809039855491 2404 34-35 0.5439272076019037 2404 36-37 0.08226282691501297 2404 38-39 0.30473406543259074 2404 40-41 0.4512870855885609 2404 42-43 0.7412153828652777 2404 44-45 -0.08892035328203463 2404 46-47 0.29572229413853535 2404 48-49 0.36921863450017156 2404 50-51 0.6750355760224167 2404 52-53 0.5873331626234091 2404 54-55 0.5089722777222789 2404 56-57 1.2638771155315212 2404 58-59 -1.4499913893296323 2404 60-61 0.15744019052842617 2404 62-63 0.114036239495789 2404 64-65 0.8870473276723274 2404 66-67 1.007068452380956 2404 68-69 1.1129712301587276 2404 70-71 1.6863591269841294 2404 72-73 2.938020833333333 2404 74-75 2.7916666666666714 2404 76-77 4.510714285714286 2404 78-79 2.838095238095228 2404 80-81 4.041666666666664 2404 82-83 9.0625 2404 84-85 12.052083333333336 2404 86-87 18.125 2404 88-89 20.375 2404 90-91 31.25 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 1.0 31 27.0 32 75.0 33 126.0 34 176.0 35 239.0 36 352.0 37 537.0 38 983.0 39 3208.0 40 3801.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.125984251968504 22.162729658792653 34.047244094488185 23.664041994750658 2 21.839563208735825 22.80554388912222 28.485930281394374 26.86896262074758 3 21.280839895013123 22.015748031496063 30.834645669291337 25.868766404199473 4 23.170603674540683 22.876640419947506 26.708661417322833 27.244094488188974 5 24.062992125984252 21.763779527559056 29.028871391076116 25.144356955380577 6 24.062992125984252 24.73490813648294 25.65879265091863 25.543307086614174 7 22.918635170603675 24.661417322834644 28.398950131233597 24.020997375328086 8 19.233595800524935 38.61417322834645 17.826771653543307 24.325459317585302 9 36.60892388451444 20.094488188976378 22.131233595800527 21.16535433070866 10-11 18.556430446194224 27.097112860892388 31.58530183727034 22.761154855643046 12-13 30.25196850393701 17.653543307086615 19.763779527559056 32.330708661417326 14-15 28.80839895013123 28.79790026246719 18.755905511811026 23.637795275590552 16-17 27.553805774278217 28.83989501312336 17.89501312335958 25.711286089238843 18-19 28.356955380577425 23.270341207349084 20.461942257217846 27.910761154855646 20-21 28.257217847769027 15.9002624671916 24.488188976377952 31.354330708661415 22-23 26.472440944881892 16.677165354330707 26.556430446194224 30.293963254593177 24-25 30.141732283464567 18.89238845144357 29.14435695538058 21.821522309711288 26-27 30.06824146981627 24.16272965879265 24.77690288713911 20.99212598425197 28-29 33.343832020997375 24.808398950131235 21.081364829396325 20.766404199475065 30-31 27.647398540452567 31.86853572741114 20.297159657688873 20.18690607444742 32-33 25.9391134057326 28.83508397127767 22.835440033232448 22.390362589757284 34-35 24.102277490568675 23.466536258208748 27.211121978482605 25.22006427273997 36-37 25.273342248092508 23.36191300243845 24.93510579721545 26.429638952253597 38-39 26.07387598194886 23.524987464482702 23.006852749456794 27.39428380411165 40-41 25.191175177990683 23.90788432803024 23.494770150303243 27.40617034367584 42-43 23.527211520478772 23.480456330652704 26.108097998877877 26.88423414999065 44-45 22.85246237329784 23.19033480086004 26.200470973686908 27.756731852155212 46-47 23.285238623751386 21.198668146503884 26.64816870144284 28.867924528301884 48-49 24.288917738699396 21.53900625516346 25.870411896612772 28.30166410952437 50-51 24.47570011302273 22.15245510485998 25.379881954037426 27.99196282807987 52-53 23.58553957801371 21.771267302781887 26.8781077812122 27.765085337992208 54-55 24.395540755755032 21.181410163602145 27.117417113073692 27.305631967569134 56-57 25.35847880299252 23.207605985037407 25.514339152119703 25.919576059850375 58-59 24.442581726739313 22.984073763621122 25.163453478625314 27.40989103101425 60-61 25.090317919075144 22.65173410404624 26.589595375722542 25.66835260115607 62-63 24.939807383627606 23.214285714285715 25.66211878009631 26.18378812199037 64-65 24.795454545454547 23.75 26.40909090909091 25.045454545454543 66-67 24.404460212873797 23.213380638621388 25.950329447541815 26.431829700963 68-69 24.783216783216783 22.76923076923077 26.99300699300699 25.454545454545453 70-71 26.595410248349577 21.848475322225717 26.878340144608615 24.677774284816095 72-73 24.120781527531083 23.410301953818827 25.435168738898756 27.033747779751334 74-75 26.32013201320132 23.96864686468647 24.834983498349835 24.876237623762375 76-77 23.986647591797805 24.74964234620887 25.65569861707201 25.608011444921313 78-79 26.225769669327253 23.888255416191562 25.598631698973772 24.287343215507413 80-81 25.954198473282442 23.4559333795975 26.092990978487162 24.496877168632896 82-83 24.59165154264973 23.049001814882033 27.404718693284934 24.9546279491833 84-85 26.380368098159508 23.803680981595093 27.73006134969325 22.085889570552148 86-87 25.042301184433164 19.289340101522843 28.25719120135364 27.411167512690355 88-89 23.822714681440445 22.99168975069252 25.761772853185594 27.42382271468144 90-91 21.935483870967744 23.870967741935484 31.61290322580645 22.58064516129032 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 3.0 17 5.5 18 4.0 19 3.0 20 5.5 21 7.0 22 8.5 23 10.0 24 12.0 25 18.0 26 26.5 27 32.5 28 31.0 29 30.0 30 34.5 31 44.5 32 55.5 33 65.5 34 75.5 35 83.0 36 99.0 37 129.0 38 173.5 39 221.0 40 290.0 41 407.5 42 563.0 43 718.5 44 891.5 45 1053.0 46 1136.0 47 1239.5 48 1330.5 49 1335.0 50 1251.5 51 1136.0 52 1023.0 53 844.0 54 683.0 55 592.5 56 518.5 57 480.5 58 449.0 59 415.5 60 399.0 61 371.0 62 343.5 63 322.5 64 304.5 65 283.0 66 252.0 67 222.5 68 202.5 69 184.0 70 163.5 71 142.5 72 120.0 73 100.5 74 91.0 75 77.5 76 55.0 77 32.5 78 24.0 79 20.5 80 16.5 81 14.5 82 9.5 83 6.0 84 6.0 85 4.5 86 2.5 87 1.0 88 0.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010498687664041995 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.2001668056713928 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 746.0 32-33 1357.0 34-35 939.0 36-37 416.0 38-39 296.0 40-41 330.0 42-43 432.0 44-45 426.0 46-47 286.0 48-49 254.0 50-51 256.0 52-53 260.0 54-55 259.0 56-57 232.0 58-59 215.0 60-61 251.0 62-63 311.0 64-65 232.0 66-67 191.0 68-69 195.0 70-71 187.0 72-73 197.0 74-75 171.0 76-77 165.0 78-79 156.0 80-81 177.0 82-83 154.0 84-85 111.0 86-87 112.0 88-89 106.0 90-91 105.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.01312335958006 #Duplication Level Percentage of deduplicated Percentage of total 1 99.35319690382781 98.37270341207349 2 0.4135298483723889 0.8188976377952757 3 0.1484466122362422 0.4409448818897638 4 0.06361997667267523 0.25196850393700787 5 0.010603329445445871 0.05249343832020997 6 0.010603329445445871 0.06299212598425197 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.04199475065616798 0.0 0.0 0.0 0.0 2 0.04199475065616798 0.0 0.0 0.0 0.0 3 0.04199475065616798 0.0 0.0 0.0 0.0 4 0.08398950131233596 0.0 0.0 0.0 0.0 5 0.25196850393700787 0.0 0.0 0.0 0.0 6 0.5564304461942258 0.0 0.0 0.0 0.0 7 1.2598425196850394 0.0 0.0 0.0 0.0 8 10.824146981627296 0.0 0.0 0.0 0.0 9 11.380577427821523 0.0 0.0 0.0 0.0 10-11 11.417322834645669 0.0 0.0 0.0 0.0 12-13 11.454068241469816 0.0 0.0 0.0 0.0 14-15 11.454068241469816 0.0 0.0 0.0 0.0 16-17 11.454068241469816 0.0 0.0 0.0 0.0 18-19 11.454068241469816 0.0 0.0 0.0 0.0 20-21 11.48031496062992 0.0 0.0 0.0 0.0 22-23 11.496062992125983 0.0 0.0 0.0 0.0 24-25 11.506561679790027 0.0 0.0 0.0 0.0 26-27 11.506561679790027 0.0 0.0 0.0 0.0 28-29 11.517060367454068 0.0 0.0 0.0 0.0 30-31 11.538057742782152 0.0 0.0 0.0 0.0 32-33 11.5748031496063 0.0 0.0 0.0 0.0 34-35 11.585301837270341 0.0 0.0 0.0 0.0 36-37 11.616797900262467 0.0 0.0 0.0 0.0 38-39 11.648293963254593 0.0 0.0 0.0 0.0 40-41 11.69028871391076 0.0 0.0 0.0 0.0 42-43 11.695538057742782 0.0 0.0 0.0 0.0 44-45 11.711286089238845 0.0 0.0 0.0 0.0 46-47 11.716535433070867 0.0 0.0 0.0 0.0 48-49 11.716535433070867 0.0 0.0 0.0 0.0 50-51 11.716535433070867 0.0 0.0 0.0 0.0 52-53 11.716535433070867 0.0 0.0 0.0 0.0 54-55 11.716535433070867 0.0 0.0 0.0 0.0 56-57 11.716535433070867 0.0 0.0 0.0 0.0 58-59 11.716535433070867 0.0 0.0 0.0 0.0 60-61 11.716535433070867 0.0 0.0 0.0 0.0 62-63 11.727034120734908 0.0 0.0 0.0 0.0 64-65 11.727034120734908 0.0 0.0 0.0 0.0 66-67 11.73228346456693 0.0 0.0 0.0 0.0 68-69 11.73753280839895 0.0 0.0 0.0 0.0 70-71 11.73753280839895 0.0 0.0 0.0 0.0 72-73 11.73753280839895 0.0 0.0 0.0 0.0 74-75 11.73753280839895 0.0 0.0 0.0 0.0 76-77 11.73753280839895 0.0 0.0 0.0 0.0 78-79 11.73753280839895 0.0 0.0 0.0 0.0 80 11.73753280839895 0.0 0.0 0.0 0.0 >>END_MODULE