##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-9C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 457337 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.27809033601043 40.0 40.0 40.0 40.0 40.0 2 39.17548766008436 40.0 40.0 40.0 40.0 40.0 3 39.186879697028665 40.0 40.0 40.0 40.0 40.0 4 39.107712693265576 40.0 40.0 40.0 40.0 40.0 5 39.23174376881818 40.0 40.0 40.0 40.0 40.0 6 39.300104736769605 40.0 40.0 40.0 40.0 40.0 7 39.3159005284943 40.0 40.0 40.0 40.0 40.0 8 39.349381309625066 40.0 40.0 40.0 40.0 40.0 9 39.370153737834464 40.0 40.0 40.0 40.0 40.0 10-11 39.36384329280159 40.0 40.0 40.0 40.0 40.0 12-13 39.3618928711213 40.0 40.0 40.0 40.0 40.0 14-15 39.366427820185116 40.0 40.0 40.0 40.0 40.0 16-17 39.4952780990823 40.0 40.0 40.0 40.0 40.0 18-19 39.50635089660359 40.0 40.0 40.0 40.0 40.0 20-21 39.51378086618839 40.0 40.0 40.0 40.0 40.0 22-23 39.51360594047715 40.0 40.0 40.0 40.0 40.0 24-25 39.515600093585256 40.0 40.0 40.0 40.0 40.0 26-27 39.502882994378325 40.0 40.0 40.0 40.0 40.0 28-29 39.50177221611197 40.0 40.0 40.0 40.0 40.0 30-31 39.487892825657646 40.0 40.0 40.0 40.0 40.0 32-33 39.4900208070957 40.0 40.0 40.0 40.0 40.0 34-35 39.48250409316754 40.0 40.0 40.0 40.0 40.0 36-37 39.47855518584546 40.0 40.0 40.0 40.0 40.0 38-39 39.4626418065847 40.0 40.0 40.0 40.0 40.0 40-41 39.451773488732236 40.0 40.0 40.0 40.0 40.0 42-43 39.44161781160902 40.0 40.0 40.0 40.0 40.0 44-45 39.421082778049275 40.0 40.0 40.0 40.0 40.0 46-47 39.42553060840767 40.0 40.0 40.0 40.0 40.0 48-49 39.41981207103479 40.0 40.0 40.0 40.0 40.0 50-51 39.412276226637815 40.0 40.0 40.0 40.0 40.0 52-53 39.41881359564738 40.0 40.0 40.0 40.0 40.0 54-55 39.37782735636209 40.0 40.0 40.0 40.0 40.0 56-57 39.385576641673225 40.0 40.0 40.0 40.0 40.0 58-59 39.367398884039446 40.0 40.0 40.0 40.0 40.0 60-61 39.32817437657311 40.0 40.0 40.0 40.0 40.0 62-63 39.38044119358466 40.0 40.0 40.0 40.0 40.0 64-65 39.37259144696444 40.0 40.0 40.0 40.0 40.0 66-67 39.36335216827428 40.0 40.0 40.0 40.0 40.0 68-69 39.36129372871357 40.0 40.0 40.0 40.0 40.0 70-71 39.38428715213577 40.0 40.0 40.0 40.0 40.0 72-73 39.381704053414026 40.0 40.0 40.0 40.0 40.0 74-75 39.36551029348985 40.0 40.0 40.0 40.0 40.0 76-77 39.36603949408793 40.0 40.0 40.0 40.0 40.0 78-79 39.36274753077669 40.0 40.0 40.0 40.0 40.0 80-81 39.328957784604384 40.0 40.0 40.0 40.0 40.0 82-83 39.347669078534224 40.0 40.0 40.0 40.0 40.0 84-85 39.32993322896618 40.0 40.0 40.0 40.0 40.0 86-87 39.31420095468876 40.0 40.0 40.0 40.0 40.0 88-89 39.376907117506995 40.0 40.0 40.0 40.0 40.0 90-91 39.550757504878355 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08571645775292325 1101 2 0.1319916678623727 1101 3 -0.00575705843677099 1101 4 0.010609730966322672 1101 5 0.13908068504437665 1101 6 0.172578411354543 1101 7 0.06039334761678816 1101 8 0.03137292160825211 1101 9 0.07063448507715009 1101 10-11 0.05684486257960941 1101 12-13 0.07814715586651033 1101 14-15 -0.09507723149458513 1101 16-17 0.06011784608790549 1101 18-19 -0.08108601689313133 1101 20-21 -0.09962343461280199 1101 22-23 0.027562984096825005 1101 24-25 0.04160903109502101 1101 26-27 -0.0034678038215005813 1101 28-29 -0.041216379635585554 1101 30-31 0.04178633073858862 1101 32-33 0.09653889768700452 1101 34-35 -0.007175282173740527 1101 36-37 -0.0580868464986537 1101 38-39 0.14720157407906953 1101 40-41 0.10590277951019544 1101 42-43 0.21503311123848334 1101 44-45 0.09119526914025045 1101 46-47 0.03265413091536118 1101 48-49 0.14180520449682632 1101 50-51 0.15665475663173822 1101 52-53 -0.00770368250162079 1101 54-55 0.15011461796765246 1101 56-57 0.005231789937269582 1101 58-59 0.21333316039992667 1101 60-61 -0.2904191692202005 1101 62-63 -0.07952039496722563 1101 64-65 0.3727050049456295 1101 66-67 0.28090116022053735 1101 68-69 0.1547183068028346 1101 70-71 -0.21182133758983213 1101 72-73 0.3441411207081728 1101 74-75 0.37188382668639974 1101 76-77 0.1582307848856459 1101 78-79 -0.16472035780780914 1101 80-81 0.19313498933374973 1101 82-83 0.03378394832379428 1101 84-85 -0.47349489745462847 1101 86-87 -0.48066058448209503 1101 88-89 0.2221332195503649 1101 90-91 -2.2458793657323 1102 1 0.47184978132700905 1102 2 0.33345555558218365 1102 3 0.1294768891194451 1102 4 0.09394519913591637 1102 5 0.11601347429943587 1102 6 -0.20869480498976145 1102 7 0.12011264714651304 1102 8 0.2062269903011824 1102 9 -0.044331936998283084 1102 10-11 0.004889657543841963 1102 12-13 -0.06339431790245698 1102 14-15 -0.1329461769464757 1102 16-17 0.011450898842760182 1102 18-19 0.048636393397998745 1102 20-21 0.03823819140048812 1102 22-23 -0.049192308772205706 1102 24-25 -0.01502329520555179 1102 26-27 -0.028551519900481992 1102 28-29 -0.08838833154687364 1102 30-31 -0.05571774069913715 1102 32-33 0.016926130639213 1102 34-35 -0.056204363355384146 1102 36-37 -0.09383019460874209 1102 38-39 -0.10102424400929522 1102 40-41 -0.08416296312926619 1102 42-43 0.00929423055205092 1102 44-45 -0.10849320372407334 1102 46-47 -0.1290760838977505 1102 48-49 -0.061203044624939196 1102 50-51 0.20232843280361124 1102 52-53 0.13288255216999545 1102 54-55 -0.2951052344008289 1102 56-57 -0.022717766723594934 1102 58-59 -0.19292697495741606 1102 60-61 -0.43027121388468004 1102 62-63 -0.3184399797734514 1102 64-65 -0.3388926014740008 1102 66-67 -0.40197897296480534 1102 68-69 -0.2812247427001324 1102 70-71 -0.3924938510214062 1102 72-73 -0.29129161780636537 1102 74-75 -0.016223491405263246 1102 76-77 -0.6918302185566034 1102 78-79 -0.043447774921176574 1102 80-81 -0.1821614894110013 1102 82-83 -0.2662339311684292 1102 84-85 -0.19657060474086308 1102 86-87 0.5206621668406584 1102 88-89 0.028193825610969725 1102 90-91 0.5541206342676972 1103 1 0.2584017480852765 1103 2 0.17971165675883327 1103 3 0.23609824224205767 1103 4 -0.021767183763529374 1103 5 0.09191587317368288 1103 6 0.09972404438253335 1103 7 -0.0738080807022854 1103 8 -0.14885772921063278 1103 9 0.16001798622535546 1103 10-11 0.027534039151142053 1103 12-13 -0.0062018990707812804 1103 14-15 -0.005554936089936291 1103 16-17 0.08994599356311284 1103 18-19 -0.07174894873253379 1103 20-21 0.0339723460418071 1103 22-23 0.0038670192243941415 1103 24-25 -0.06184316059245987 1103 26-27 -0.052424323366253134 1103 28-29 -0.031084398915368183 1103 30-31 -0.007864160088374206 1103 32-33 0.081511840529636 1103 34-35 0.06796917222424526 1103 36-37 -0.015521899683044182 1103 38-39 -0.02470722409106685 1103 40-41 0.022092100320058705 1103 42-43 0.04747319533244365 1103 44-45 -0.06535438244939229 1103 46-47 -0.011605668270142644 1103 48-49 -0.021154015639744728 1103 50-51 0.004273407181663913 1103 52-53 0.12107379081896141 1103 54-55 0.0149406868563986 1103 56-57 -0.06774042602812358 1103 58-59 -0.22373104858796466 1103 60-61 -0.08338443639587467 1103 62-63 0.18720430886111217 1103 64-65 0.1053789344132241 1103 66-67 0.18438594680311837 1103 68-69 0.1532674629606845 1103 70-71 -0.08124856550562498 1103 72-73 -0.1633200609816825 1103 74-75 -0.04586641952343484 1103 76-77 -0.11262573484727767 1103 78-79 -0.31648045440151407 1103 80-81 -0.7900519886058532 1103 82-83 -0.18642721306384402 1103 84-85 0.0795516975274495 1103 86-87 0.816958463136956 1103 88-89 0.7554665528836964 1103 90-91 0.5541206342676972 1104 1 0.2859820109596569 1104 2 0.06824142764154573 1104 3 0.3175749354834565 1104 4 0.2620222294376475 1104 5 0.10139658853675115 1104 6 -0.07770898010931404 1104 7 0.0024398543691788177 1104 8 0.021680229562619502 1104 9 8.568858877850971E-4 1104 10-11 -0.009555668526800787 1104 12-13 -0.056909361542949455 1104 14-15 -0.07605698306257125 1104 16-17 -0.12319772546295837 1104 18-19 -0.018254730532433427 1104 20-21 -0.049343031391217096 1104 22-23 0.14047550877405968 1104 24-25 -0.03986513861443797 1104 26-27 -0.09520246026203694 1104 28-29 0.021375559426942914 1104 30-31 -0.0024055663944864136 1104 32-33 -0.042204291565020924 1104 34-35 -0.13535115820982213 1104 36-37 0.10947573515213804 1104 38-39 -0.127487181488128 1104 40-41 -0.07177252494235375 1104 42-43 -0.14245983011817742 1104 44-45 -0.11128107225627559 1104 46-47 0.026404985741891096 1104 48-49 0.02856107445202838 1104 50-51 0.002384815431284437 1104 52-53 -0.0023710293740606403 1104 54-55 0.06416220499286851 1104 56-57 -0.13763630651309455 1104 58-59 -0.05593513543131223 1104 60-61 -0.05267260449207356 1104 62-63 -0.027028517852684786 1104 64-65 0.28670836215543005 1104 66-67 -0.11848224093866122 1104 68-69 -0.12474380918101957 1104 70-71 0.031472432246268056 1104 72-73 0.32216190712193793 1104 74-75 -0.3970455392757799 1104 76-77 -0.1588026810242198 1104 78-79 -0.14622495775832078 1104 80-81 -0.3772385540532923 1104 82-83 -0.44977789746715047 1104 84-85 0.36185863789890504 1104 86-87 0.816958463136956 1104 88-89 -0.815962018544873 1104 90-91 0.5541206342676972 1201 1 0.35719657936928684 1201 2 0.37805439076433345 1201 3 0.13792418606190893 1201 4 0.13636764146537672 1201 5 0.14625913636451315 1201 6 0.08663688399612113 1201 7 0.2798709022442054 1201 8 0.08828853710696904 1201 9 0.07848273815700679 1201 10-11 0.10613841459795736 1201 12-13 0.07498225488144072 1201 14-15 -0.015095537071474041 1201 16-17 0.09609777307600353 1201 18-19 0.13066953001227688 1201 20-21 -0.029550247373400396 1201 22-23 0.07739833910929406 1201 24-25 0.14536342776494848 1201 26-27 0.060678865229796486 1201 28-29 0.04689411310706504 1201 30-31 0.01385279190586175 1201 32-33 0.12026831006279082 1201 34-35 0.04144484520897862 1201 36-37 0.11159924254304343 1201 38-39 0.13457525918302338 1201 40-41 0.08767349600097418 1201 42-43 0.0827530363533171 1201 44-45 0.08249582823102486 1201 46-47 0.13628439460529762 1201 48-49 -0.03336365143703546 1201 50-51 0.1036053965780468 1201 52-53 -0.018545057807834553 1201 54-55 -0.03340277101364819 1201 56-57 -0.1459334400150425 1201 58-59 -0.09618470736027973 1201 60-61 0.1770584452192594 1201 62-63 -0.1202019955969007 1201 64-65 0.15448500372326635 1201 66-67 0.13480920455297962 1201 68-69 -0.3484991601836498 1201 70-71 -0.22682191013076647 1201 72-73 -0.27428374470078154 1201 74-75 -0.2334617954334206 1201 76-77 -0.3731443058813255 1201 78-79 -0.056272869223334965 1201 80-81 -0.5303568947008159 1201 82-83 -0.6628502609154552 1201 84-85 0.6042828803231473 1201 86-87 0.15029179647029167 1201 88-89 -0.6195334471163036 1201 90-91 -0.5887365085894416 1202 1 0.09383216354304835 1202 2 0.05698182541066643 1202 3 0.04372457244020467 1202 4 0.31177493910827536 1202 5 0.284286202342912 1202 6 -0.02589376386181641 1202 7 0.2102058230106607 1202 8 -0.14231008928405942 1202 9 -0.016821741913965127 1202 10-11 0.11613095674942997 1202 12-13 0.013805149664030125 1202 14-15 -0.0336914142948217 1202 16-17 0.079222562867038 1202 18-19 0.13077189263919564 1202 20-21 -0.059024184711226724 1202 22-23 0.05523926758706921 1202 24-25 0.07525640195169814 1202 26-27 0.07091350311794287 1202 28-29 -0.012445339236641928 1202 30-31 0.12355465669295995 1202 32-33 -4.571539161517535E-4 1202 34-35 -0.025716057417362492 1202 36-37 -0.19723268401565264 1202 38-39 0.14165849311050493 1202 40-41 -0.014778826921407529 1202 42-43 0.04959451892739963 1202 44-45 0.033371658805364746 1202 46-47 -0.029049871911965397 1202 48-49 0.10343933054374332 1202 50-51 0.11254149941233038 1202 52-53 -0.043655138444989916 1202 54-55 0.024831460411540718 1202 56-57 0.11981717135066106 1202 58-59 0.36873086870063077 1202 60-61 0.05628978564057263 1202 62-63 -0.03400781963682675 1202 64-65 -0.07119035288613418 1202 66-67 -0.04220667694963254 1202 68-69 -0.1657415416073178 1202 70-71 -0.1593269900091414 1202 72-73 0.05153802020150522 1202 74-75 0.1462383984475082 1202 76-77 -0.17734044956198858 1202 78-79 -0.2634031818330982 1202 80-81 -0.6620408692903865 1202 82-83 -0.3753359787167909 1202 84-85 -0.2909552149149519 1202 86-87 0.10728104378211611 1202 88-89 -0.24453344711630365 1202 90-91 -1.4458793657323028 1203 1 0.11123708257056109 1203 2 0.18413896957030573 1203 3 0.21811330954606944 1203 4 0.328236672494846 1203 5 0.03241790878581696 1203 6 0.004193858084228452 1203 7 -1.7487815354400027E-4 1203 8 -0.03633375066784339 1203 9 -0.08973627986303967 1203 10-11 -0.02746276917969226 1203 12-13 0.06698824400461234 1203 14-15 0.05555281105110055 1203 16-17 0.023624913285651417 1203 18-19 -0.036898218751026945 1203 20-21 -0.03684989029267882 1203 22-23 0.0015877243781545758 1203 24-25 0.0067276772330018275 1203 26-27 0.08721489494555357 1203 28-29 -0.0649964991977825 1203 30-31 -0.07857317011301035 1203 32-33 0.05076657706053567 1203 34-35 0.032388596845130735 1203 36-37 -0.029257334963396886 1203 38-39 -0.0443401129988672 1203 40-41 -0.12251526763039777 1203 42-43 -0.08274783202331548 1203 44-45 0.027174131324002815 1203 46-47 0.044494825165443785 1203 48-49 -0.1263564642941688 1203 50-51 -0.054812976055828244 1203 52-53 -0.03898462321216556 1203 54-55 -0.16471558281496357 1203 56-57 -0.23003059381353808 1203 58-59 -0.02296656949327769 1203 60-61 0.15684793470285285 1203 62-63 0.14296859114767813 1203 64-65 -0.40436200538552214 1203 66-67 -0.22653079369310092 1203 68-69 -0.435034664216289 1203 70-71 -0.4733513908877498 1203 72-73 -0.6343735550906047 1203 74-75 -0.3820421040137276 1203 76-77 -0.6242552023591017 1203 78-79 0.07279187311195301 1203 80-81 -0.09158665307345615 1203 82-83 -0.22824295546097773 1203 84-85 -0.011101735061465945 1203 86-87 0.058337773481781596 1203 88-89 0.08879988621702495 1203 90-91 0.5541206342676972 1204 1 0.2378201248780556 1204 2 0.009390710068281294 1204 3 -0.009521951472052592 1204 4 -0.05799019943618333 1204 5 -0.1843587568500027 1204 6 -0.028071165260698194 1204 7 0.045552510458357176 1204 8 -0.16719204433869095 1204 9 -0.1690924276111403 1204 10-11 -0.07366535333832758 1204 12-13 -0.1463428767780286 1204 14-15 -0.17286237408659133 1204 16-17 -0.1658287626913264 1204 18-19 -0.11909950555040183 1204 20-21 -0.03292914989276596 1204 22-23 -0.036589334991113276 1204 24-25 -0.11286923625877421 1204 26-27 -0.03200940489529103 1204 28-29 -0.1515333796521574 1204 30-31 -0.11614529800849027 1204 32-33 -0.264765364872126 1204 34-35 -0.12055548030618723 1204 36-37 -0.10952913928964847 1204 38-39 -0.14146269089209795 1204 40-41 -0.010701217928463791 1204 42-43 -0.07120349923977187 1204 44-45 -0.16164694951572756 1204 46-47 -0.32798489646681617 1204 48-49 0.004687455314503097 1204 50-51 -0.2877401024531281 1204 52-53 -0.1304599622559195 1204 54-55 -0.23235219559943943 1204 56-57 -0.3503702242382971 1204 58-59 -0.19121115932649957 1204 60-61 0.06506055671480482 1204 62-63 0.17596083240078997 1204 64-65 0.046611853471745235 1204 66-67 -0.12730270470891014 1204 68-69 -0.2534033290537039 1204 70-71 0.27063103825357615 1204 72-73 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0.27457144781753584 2401 56-57 0.2606915847553992 2401 58-59 0.32395302818513017 2401 60-61 0.0642825743216946 2401 62-63 0.16903254329858441 2401 64-65 0.28106284132964277 2401 66-67 0.20039312529978304 2401 68-69 0.12101627926563907 2401 70-71 0.04692195164127355 2401 72-73 0.2634103642634358 2401 74-75 0.25941554154788093 2401 76-77 -0.026113751165482313 2401 78-79 0.07933513954343852 2401 80-81 0.06353355628404245 2401 82-83 0.17236032605156737 2401 84-85 0.2638573484082585 2401 86-87 0.36534555991114814 2401 88-89 0.3554665528836978 2401 90-91 0.5541206342676972 2402 1 0.26845333852496367 2402 2 0.2606824801105745 2402 3 0.29281393093049246 2402 4 0.1948798335404618 2402 5 0.2896917777444017 2402 6 0.20832563738098742 2402 7 0.20934744279632156 2402 8 0.16033923464902244 2402 9 0.07591292055206367 2402 10-11 0.15650627609831247 2402 12-13 0.10010936035197915 2402 14-15 0.1324125570086494 2402 16-17 0.11964598110724722 2402 18-19 0.03222466415281389 2402 20-21 -0.0019604000314572545 2402 22-23 0.07020172270247826 2402 24-25 0.12953970072165788 2402 26-27 0.12204077463433549 2402 28-29 0.0992550860685597 2402 30-31 0.027134879168826842 2402 32-33 0.02112598355506634 2402 34-35 0.07620687626577904 2402 36-37 0.14981687247476572 2402 38-39 0.12565206204165946 2402 40-41 0.1263160701204029 2402 42-43 0.14894285430403897 2402 44-45 0.1624089647066711 2402 46-47 0.19580190132231223 2402 48-49 0.18762152739522975 2402 50-51 0.12005383744015319 2402 52-53 0.20824305025924872 2402 54-55 0.17974248288135186 2402 56-57 0.18519630943617216 2402 58-59 0.26802964661836626 2402 60-61 0.38558179840983087 2402 62-63 0.06370559653647234 2402 64-65 0.035368080173455496 2402 66-67 0.3270198128613089 2402 68-69 0.3035731082645299 2402 70-71 0.3821649122198991 2402 72-73 0.29170873300591893 2402 74-75 0.19291844087162247 2402 76-77 -0.14178876884901825 2402 78-79 0.13317536861542578 2402 80-81 0.5929707857212634 2402 82-83 0.3217955986072454 2402 84-85 0.6042828803231473 2402 86-87 0.39590583155801085 2402 88-89 -0.2748364774193348 2402 90-91 0.5541206342676972 2403 1 -0.2331666534688921 2403 2 -0.0666476396479041 2403 3 -0.06621757655858573 2403 4 -0.07292339893777466 2403 5 0.0553996537827004 2403 6 0.046979021642250984 2403 7 0.05285037838027051 2403 8 0.1232274489466505 2403 9 0.10813137136435813 2403 10-11 0.10633343525366712 2403 12-13 0.07641515312644032 2403 14-15 0.1110388788553962 2403 16-17 0.14777275329926454 2403 18-19 0.1042538448580217 2403 20-21 0.07426574834896371 2403 22-23 0.0689987170648152 2403 24-25 -0.021003840993657263 2403 26-27 0.04483366585093762 2403 28-29 0.05485333639381196 2403 30-31 0.03861034369163008 2403 32-33 0.04131015949204908 2403 34-35 0.11829238857139757 2403 36-37 0.03977496145570569 2403 38-39 0.05864334315216979 2403 40-41 0.11864149616766184 2403 42-43 0.019185348447997796 2403 44-45 0.04307516806883882 2403 46-47 -0.006551411169851917 2403 48-49 -0.006792306504145529 2403 50-51 0.14483291213834093 2403 52-53 0.03669228241403033 2403 54-55 -0.022287569237349203 2403 56-57 -0.13533091598133495 2403 58-59 -0.22066799426662698 2403 60-61 -0.12843260892784514 2403 62-63 -0.004614121939439997 2403 64-65 0.14689601600295532 2403 66-67 -0.03232529860156319 2403 68-69 0.22867258230164111 2403 70-71 0.17507062944748242 2403 72-73 0.189696391517586 2403 74-75 0.23953063951023523 2403 76-77 0.39913685418803624 2403 78-79 0.08764576921585387 2403 80-81 0.4799098463114575 2403 82-83 0.3530599664233378 2403 84-85 0.6042828803231473 2403 86-87 0.816958463136956 2403 88-89 0.7554665528836964 2403 90-91 0.5541206342676972 2404 1 -0.24025505251892554 2404 2 0.0023647225357663615 2404 3 -0.11897803172600874 2404 4 -0.21639884757809114 2404 5 -0.10708796044167457 2404 6 -0.05891051296714522 2404 7 -0.11130931555345569 2404 8 -0.16647387692114535 2404 9 0.1467740083156457 2404 10-11 -0.017443223653870632 2404 12-13 0.12700269107016737 2404 14-15 0.07385157054001468 2404 16-17 0.09153297647914371 2404 18-19 0.039830609142946116 2404 20-21 0.02097693172135706 2404 22-23 0.03342696607002438 2404 24-25 0.005957338446748395 2404 26-27 -0.06711506883394946 2404 28-29 -0.00870092903139863 2404 30-31 0.028088332398951366 2404 32-33 0.034757139642017876 2404 34-35 -0.03616299586753513 2404 36-37 -0.050363258439823255 2404 38-39 -0.08292382042771607 2404 40-41 0.016183152022385627 2404 42-43 0.0386254818307421 2404 44-45 0.007248117470012971 2404 46-47 0.031245148209649187 2404 48-49 0.06321151930574587 2404 50-51 -0.1292707219870266 2404 52-53 -0.059114383191811726 2404 54-55 0.07818866809103042 2404 56-57 0.18026346565621054 2404 58-59 0.15097238531102875 2404 60-61 0.005491578810890019 2404 62-63 0.08371704170872363 2404 64-65 0.059059569224260144 2404 66-67 -0.06461104770892234 2404 68-69 -0.03473073463355547 2404 70-71 -0.08328063989912238 2404 72-73 0.09650944204201295 2404 74-75 0.20383810844876393 2404 76-77 0.20918599188436104 2404 78-79 0.07545375562196455 2404 80-81 0.188313922976171 2404 82-83 0.280945971027613 2404 84-85 0.31856859460886255 2404 86-87 0.816958463136956 2404 88-89 0.3038536496578885 2404 90-91 0.08353239897358122 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 6.0 31 236.0 32 1651.0 33 3150.0 34 4263.0 35 5254.0 36 7738.0 37 11886.0 38 32096.0 39 177717.0 40 213339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.401653055200235 11.680715449287721 32.5157707151213 29.40186078039074 2 26.5297509583152 17.846638954064282 29.9158344503655 25.707775637255008 3 26.777030454632918 19.343108786537687 22.80598946944916 31.07387128938024 4 26.347091969379257 20.55508301318284 21.82132650539974 31.27649851203817 5 27.252988496447916 20.824249951348786 21.32869197112851 30.59406958107479 6 27.70232891718798 22.940195085899457 23.639897930847493 25.717578066065066 7 26.217209628785774 22.59843397757015 23.095222997483255 28.089133396160822 8 25.396152071667068 23.677725615902496 23.25877853748986 27.667343774940576 9 24.978735593227753 23.858336412754706 23.8937588692802 27.269169124737335 10-11 24.77614975390139 22.355177910381187 23.91114211183438 28.957530223883044 12-13 25.209856189199648 22.175988384932772 23.741136186225912 28.873019239641668 14-15 25.73572660860591 21.935684189120934 23.354659692961647 28.973929509311514 16-17 25.74009975138683 21.563529738464197 23.20105305278165 29.495317457367324 18-19 25.793452093314123 21.988052573922513 23.071826683605305 29.14666864915806 20-21 25.99756853261381 22.007403730728107 22.991142199297236 29.00388553736085 22-23 25.555225140323216 21.617428723239097 23.240520666379496 29.586825470058187 24-25 25.821221549972996 22.076171400958156 23.265666237369818 28.83694081169903 26-27 26.001285703977594 22.238305669560958 23.17536083894371 28.585047787517738 28-29 26.03714547478118 21.842426919317703 23.280206937116393 28.840220668784728 30-31 26.249633746889582 22.059698163705303 23.408930890771295 28.28173719863382 32-33 26.129743419224656 21.996916293539705 23.56268305236835 28.310657234867293 34-35 26.036063081340455 21.808528666953958 23.798583236090195 28.35682501561539 36-37 26.141571889322694 22.12561275383202 23.847867299192323 27.88494805765296 38-39 26.24786489652261 22.137703341524304 23.75716709857958 27.857264663373506 40-41 26.093031237708193 21.78742442673665 23.94273365255966 28.1768106829955 42-43 26.07146123847299 22.066006436001693 24.220154497301703 27.642377828223612 44-45 26.189357798165137 22.076880733944954 24.18605504587156 27.547706422018347 46-47 26.15525616350472 21.90470371388711 24.274585280909175 27.665454841699 48-49 26.217328242015213 22.23057113386569 24.236586904200795 27.3155137199183 50-51 26.268064969945005 22.09464125847295 24.189794091316024 27.44749968026602 52-53 26.274957051494894 21.939684418715444 24.18686569749816 27.598492832291498 54-55 26.418539509679444 22.262398722496368 24.199924082775954 27.119137685048234 56-57 26.466579272743523 22.037048071623293 24.207123597848916 27.289249057784264 58-59 26.68118137872788 21.737691142205982 24.081265411271396 27.49986206779474 60-61 26.610279765777488 22.302605385564046 23.834471105759427 27.252643742899036 62-63 26.77041197249802 22.162604887507104 23.809046234078828 27.257936905916047 64-65 27.00793050875687 22.0612916864182 23.63201926895827 27.298758535866657 66-67 27.08310259534094 21.96108834496253 23.726510872374202 27.229298187322332 68-69 27.100437876299942 22.13242337164751 23.461446360153257 27.30569239189929 70-71 27.185587020355346 22.132085801025234 23.230975961777734 27.451351216841687 72-73 26.9167703596888 22.49305159842309 23.117535556046562 27.472642485841543 74-75 27.270603753881666 22.238863392783532 23.027563274615957 27.462969578718845 76-77 27.66113520033906 21.88895771525446 22.356795879111925 28.09311120529456 78-79 27.539073990547724 22.732531916145355 22.14421587276685 27.584178220540075 80-81 27.931390683931102 22.18512023446321 22.14428135585077 27.739207725754916 82-83 28.28298209282495 22.137288342063588 21.680472651967353 27.89925691314411 84-85 28.262003128635033 22.49007260037704 20.821467249608922 28.426457021379004 86-87 29.480878947071115 21.60085861153477 19.883635541998533 29.034626899395583 88-89 27.539440907832823 21.072054617584648 19.946489528554295 31.44201494602823 90-91 25.86015538290788 24.30632630410655 20.288568257491676 29.544950055493896 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.0 9 3.5 10 4.5 11 8.0 12 12.0 13 16.0 14 22.0 15 34.5 16 52.5 17 77.5 18 111.5 19 157.5 20 221.5 21 299.5 22 376.5 23 485.5 24 629.5 25 789.5 26 1030.5 27 1362.5 28 1712.0 29 2094.5 30 2544.5 31 3026.0 32 3603.0 33 4266.0 34 5150.0 35 6186.5 36 7330.5 37 8513.0 38 9846.0 39 11488.0 40 13149.0 41 14890.5 42 16728.5 43 18651.5 44 20729.0 45 22774.5 46 24751.0 47 26717.5 48 28505.0 49 30429.0 50 32203.0 51 33396.0 52 34178.0 53 34866.5 54 35447.0 55 35618.0 56 35652.0 57 35520.0 58 35200.0 59 34552.0 60 33764.0 61 32937.0 62 31865.0 63 30484.0 64 28639.0 65 26603.5 66 24259.0 67 21988.0 68 19783.5 69 17422.0 70 15165.5 71 12894.0 72 10714.5 73 8624.5 74 6766.5 75 5477.5 76 4387.0 77 3293.0 78 2498.5 79 1835.5 80 1255.0 81 876.5 82 635.5 83 434.5 84 292.5 85 203.5 86 129.5 87 78.0 88 49.0 89 34.5 90 22.5 91 15.0 92 10.5 93 6.0 94 5.0 95 4.5 96 3.5 97 2.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.373142780925226E-4 2 0.005247771337110271 3 2.186571390462613E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 12017.0 32-33 25506.0 34-35 27107.0 36-37 27813.0 38-39 28515.0 40-41 28699.0 42-43 28251.0 44-45 27296.0 46-47 26345.0 48-49 24484.0 50-51 22789.0 52-53 20898.0 54-55 18924.0 56-57 17046.0 58-59 15104.0 60-61 13615.0 62-63 12169.0 64-65 10593.0 66-67 9497.0 68-69 8534.0 70-71 7396.0 72-73 6820.0 74-75 5899.0 76-77 5302.0 78-79 4740.0 80-81 4524.0 82-83 4073.0 84-85 3640.0 86-87 3494.0 88-89 3216.0 90-91 3031.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.8898965122784 #Duplication Level Percentage of deduplicated Percentage of total 1 99.89326313824411 99.78327717152999 2 0.10394668277102796 0.20766446769585228 3 0.002092370600754477 0.006270200483240955 4 6.978083840978456E-4 0.0027881602909173605 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013119428342775678 0.0 0.0 0.0 0.0 2 0.0013119428342775678 0.0 0.0 0.0 0.0 3 0.0013119428342775678 0.0 0.0 0.0 0.0 4 0.0013119428342775678 0.0 0.0 0.0 0.0 5 0.001530599973323829 0.0 0.0 0.0 0.0 6 0.0017492571123700904 0.0 0.0 0.0 0.0 7 0.0021865713904626128 0.0 0.0 0.0 0.0 8 0.008964942700896712 0.0 0.0 0.0 2.186571390462613E-4 9 0.009402256978989236 0.0 0.0 0.0 2.186571390462613E-4 10-11 0.009402256978989236 0.0 0.0 0.0 2.186571390462613E-4 12-13 0.009402256978989236 0.0 0.0 0.0 2.186571390462613E-4 14-15 0.009402256978989236 0.0 0.0 0.0 2.186571390462613E-4 16-17 0.009402256978989236 0.0 0.0 0.0 2.186571390462613E-4 18-19 0.009402256978989236 0.0 0.0 0.0 2.186571390462613E-4 20-21 0.009402256978989236 0.0 0.0 0.0 2.186571390462613E-4 22-23 0.009511585548512367 0.0 0.0 0.0 2.186571390462613E-4 24-25 0.009839571257081759 0.0 0.0 0.0 4.373142780925226E-4 26-27 0.01016755696565115 0.0 0.0 0.0 4.373142780925226E-4 28-29 0.010276885535174281 0.0 0.0 0.0 5.466428476156532E-4 30-31 0.010495542674220542 0.0 0.0 0.0 6.559714171387839E-4 32-33 0.010714199813266803 0.0 0.0 0.0 6.559714171387839E-4 34-35 0.010932856952313065 0.0 0.0 0.0 7.652999866619146E-4 36-37 0.011370171230405587 0.0 0.0 0.0 0.0010932856952313064 38-39 0.01180748550849811 0.0 0.0 0.0 0.0010932856952313064 40-41 0.01202614264754437 0.0 0.0 0.0 0.0010932856952313064 42-43 0.012463456925636893 0.0 0.0 0.0 0.0010932856952313064 44-45 0.012682114064683154 0.0 0.0 0.0 0.0010932856952313064 46-47 0.013010099773252546 0.0 0.0 0.0 0.0010932856952313064 48-49 0.013119428342775678 0.0 0.0 0.0 0.0010932856952313064 50-51 0.01333808548182194 0.0 0.0 0.0 0.0010932856952313064 52-53 0.013994056898960723 0.0 0.0 0.0 0.0010932856952313064 54-55 0.014322042607530115 0.0 0.0 0.0 0.0010932856952313064 56-57 0.014650028316099507 0.0 0.0 0.0 0.0010932856952313064 58-59 0.014650028316099507 0.0 0.0 0.0 0.0010932856952313064 60-61 0.014650028316099507 0.0 0.0 0.0 0.0010932856952313064 62-63 0.014650028316099507 0.0 0.0 0.0 0.0010932856952313064 64-65 0.014650028316099507 0.0 0.0 0.0 0.0010932856952313064 66-67 0.014650028316099507 0.0 0.0 0.0 0.0010932856952313064 68-69 0.014650028316099507 0.0 0.0 0.0 0.0010932856952313064 70-71 0.014650028316099507 0.0 0.0 0.0 0.0010932856952313064 72-73 0.014868685455145768 0.0 0.0 0.0 0.0010932856952313064 74-75 0.014868685455145768 0.0 0.0 0.0 0.0010932856952313064 76-77 0.014868685455145768 0.0 0.0 0.0 0.0010932856952313064 78-79 0.014868685455145768 0.0 0.0 0.0 0.0010932856952313064 80 0.014868685455145768 0.0 0.0 0.0 0.0010932856952313064 >>END_MODULE